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Artykuły w czasopismach na temat "Degrees De-Acetylated"

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Romany, Aarion, Gregory F. Payne, and Jana Shen. "Effect of Acetylation on the Nanofibril Formation of Chitosan from All-Atom De Novo Self-Assembly Simulations." Molecules 29, no. 3 (2024): 561. http://dx.doi.org/10.3390/molecules29030561.

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Chitosan-based materials have broad applications, from biotechnology to pharmaceutics. Recent experiments showed that the degree and pattern of acetylation along the chitosan chain modulate its biological and physicochemical properties; however, the molecular mechanism remains unknown. Here, we report, to the best of our knowledge, the first de novo all-atom molecular dynamics (MD) simulations to investigate chitosan’s self-assembly process at different degrees and patterns of acetylation. Simulations revealed that 10 mer chitosan chains with 50% acetylation in either block or alternating patt
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Naqvi, Shoa, Stefan Cord-Landwehr, Ratna Singh, et al. "A Recombinant Fungal Chitin Deacetylase Produces Fully Defined Chitosan Oligomers with Novel Patterns of Acetylation." Applied and Environmental Microbiology 82, no. 22 (2016): 6645–55. http://dx.doi.org/10.1128/aem.01961-16.

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ABSTRACTPartially acetylated chitosan oligosaccharides (paCOS) are potent biologics with many potential applications, and their bioactivities are believed to be dependent on their structure, i.e., their degrees of polymerization and acetylation, as well as their pattern of acetylation. However, paCOS generated via chemicalN-acetylation or de-N-acetylation of GlcN or GlcNAc oligomers, respectively, typically display random patterns of acetylation, making it difficult to control and predict their bioactivities. In contrast, paCOS produced from chitin deacetylases (CDAs) acting on chitin oligomer
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De Rijke, Y. B., E. A. L. Biessen, C. J. M. Vogelezang, and T. J. C. van Berkel. "Binding characteristics of scavenger receptors on liver endothelial and Kupffer cells for modified low-density lipoproteins." Biochemical Journal 304, no. 1 (1994): 69–73. http://dx.doi.org/10.1042/bj3040069.

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Previous studies showed that both endothelial and Kupffer cells contain specific recognition sites of oxidized low-density lipoprotein (OxLDL), in addition to recognition sites which recognize OxLDL and acetylated LDL (AcLDL). We have determined the binding characteristics of the recognition sites for OxLDL on Kupffer cells and endothelial cells (OxLDL-specific binding-site) in comparison to the recognition site for AcLDL on endothelial cells, which recognizes both AcLDL and OxLDL (Ac/OxLDL binding site). The capacity of Kupffer cells to bind OxLDL (Bmax. = 779 ng of 125I-OxLDL/mg of cell prot
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WITCZAK, TERESA, KAROLINA PYCIA, AGNIESZKA WIKIERA, and MARIUSZ WITCZAK. "WYKRESY FAZOWE HYDROLIZATÓW O RÓŻNYM STOPNIU DEPOLIMERYZACJI OTRZYMANYCH NA BAZIE SKROBI MODYFIKOWANYCH CHEMICZNIE." Zywnosc Nauka Technologia Jakosc/Food Science Technology Quality 30, no. 4 (2023): 264–87. http://dx.doi.org/10.15193/zntj/2023/137/483.

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Background. One of the best ways to present the relationship between phase changes and the stability of food products related to water content and temperature change is a phase diagram. The aim of the pre-sented research was to develop phase diagrams for maltodextrins obtained from native potato starch and acetylated potato starch (E14120) and distarch phosphate (E1412). For this purpose, glass transition curves and freezing curves were determined using the DSC (differential scanning calorimetry) method. As part of the research, an attempt was also made to assess the impact of modification of
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DROUILLARD, Sophie, Sylvie ARMAND, J. Gideon DAVIES, E. Constantin VORGIAS, and Bernard HENRISSAT. "Serratia marcescens chitobiase is a retaining glycosidase utilizing substrate acetamido group participation." Biochemical Journal 328, no. 3 (1997): 945–49. http://dx.doi.org/10.1042/bj3280945.

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The stereochemistry of the reaction catalysed by Serratia marcescens chitobiase was determined by HPLC separation of the anomers of N-acetylglucosamine produced during the hydrolysis of p-nitrophenyl N-acetyl-β-D-glucosaminide (PNP-GlcNAc). In the early stages of the reaction, the β-anomer was found to prevail, whereas the α-anomer dominated at mutarotation equilibrium. This established that chitobiase hydrolyses glycosidic bonds with overall retention of the anomeric configuration. Chitobiase-catalysed hydrolysis of PNP-GlcNAc was competitively inhibited by a series of chito-oligosaccharides
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Adi-Dako, Ofosua, Kwabena Ofori-Kwakye, Samuel Frimpong Manso, Mariam EL Boakye-Gyasi, Clement Sasu, and Mike Pobee. "Physicochemical and Antimicrobial Properties of Cocoa Pod Husk Pectin Intended as a Versatile Pharmaceutical Excipient and Nutraceutical." Journal of Pharmaceutics 2016 (March 14, 2016): 1–12. http://dx.doi.org/10.1155/2016/7608693.

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The physicochemical and antimicrobial properties of cocoa pod husk (CPH) pectin intended as a versatile pharmaceutical excipient and nutraceutical were studied. Properties investigated include pH, moisture content, ash values, swelling index, viscosity, degree of esterification (DE), flow properties, SEM, FTIR, NMR, and elemental content. Antimicrobial screening and determination of MICs against test microorganisms were undertaken using agar diffusion and broth dilution methods, respectively. CPH pectin had a DE of 26.8% and exhibited good physicochemical properties. Pectin had good microbiolo
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Gustiansyah, Teguh, and Dewi Astuti Herawati. "Cellullosa Acetate Production From Mollases." Jurnal Kimia dan Rekayasa 2, no. 2 (2022): 50–57. http://dx.doi.org/10.31001/jkireka.v2i2.35.

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Previous research stated that cellulose acetate is made from wood containing cellulose. This research utilizes molasses as a producer of cellulose from nata de molasses. Nata de molasses is then acetylated to produce cellulose acetate. The aim of the study was to determine the effect of molasses content on acetyl content and degree of substitution of cellulose acetate. The manufacture of cellulose acetate goes through 3 stages, namely making nata de molasses, pollinating nata de molasses, and making cellulose acetate. Making Nata de molasses using molasses as much as 1000 ml with molasses leve
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Lewis, Amanda L., Hongzhi Cao, Silpa K. Patel, et al. "NeuA Sialic Acid O-Acetylesterase Activity Modulates O-Acetylation of Capsular Polysaccharide in Group B Streptococcus." Journal of Biological Chemistry 282, no. 38 (2007): 27562–71. http://dx.doi.org/10.1074/jbc.m700340200.

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Group B Streptococcus (GBS) is a common cause of neonatal sepsis and meningitis. A major GBS virulence determinant is its sialic acid (Sia)-capped capsular polysaccharide. Recently, we discovered the presence and genetic basis of capsular Sia O-acetylation in GBS. We now characterize a GBS Sia O-acetylesterase that modulates the degree of GBS surface O-acetylation. The GBS Sia O-acetylesterase operates cooperatively with the GBS CMP-Sia synthetase, both part of a single polypeptide encoded by the neuA gene. NeuA de-O-acetylation of free 9-O-acetyl-N-acetylneuraminic acid (Neu5,9Ac2) was enhanc
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Nampally, Malathi, Bruno Maria Moerschbacher, and Stephan Kolkenbrock. "Fusion of a Novel Genetically Engineered Chitosan Affinity Protein and Green Fluorescent Protein for Specific Detection of ChitosanIn VitroandIn Situ." Applied and Environmental Microbiology 78, no. 9 (2012): 3114–19. http://dx.doi.org/10.1128/aem.07506-11.

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ABSTRACTChitin is the second most abundant polysaccharide, present, e.g., in insect and arthropod exoskeletons and fungal cell walls. In some species or under specific conditions, chitin appears to be enzymatically de-N-acetylated to chitosan—e.g., when pathogenic fungi invade their host tissues. Here, the deacetylation of chitin is assumed to represent a pathogenicity mechanism protecting the fungus from the host's chitin-driven immune response. While highly specific chitin binding lectins are well known and easily available, this is not the case for chitosan-specific probes. This is partly d
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SR, Grobler, Olivier A, Kruijsse HW, and Perchyonok VT. "Cytotoxicity of Different Degrees of De-Acetylated Chitosan on 3T3- and Two Human Tooth Fibroblast Cell-Lines." October 17, 2016. https://doi.org/10.19070/2377-8075-1600068.

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Nowadays chitosan becomes more and more important as a drug carrier. In this study the influence of different degrees of de-acetylation of chitosan on the cytotoxicity were investigated. Materials and Methods: Chitosan (87%, 70% and 40% de-acetylated) was analysed for their cytotoxic effect on mouse Balb/c 3T3 fibroblast cells as well as two different human tooth pulp fibroblast cell-lines. The cell survival rate was determined over a 24 hour period according to the standard MTT assay. Results: The Univariate ANOVA test showed significant differences in the cell survival rates (p<0.01) amon
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