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Rozprawy doktorskie na temat „Direct sequencing”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 11 marca 2023

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1

Fillion, Bergeron Marianne. "Plasmid isolation and purification by electrofiltration and comparison of different direct colony sequencing methods and PCR-based sequencing methods." Thesis, University of Ottawa (Canada), 2002. http://hdl.handle.net/10393/6050.

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We have designed an electrophoresis system that can purify plasmid DNA from a culture without centrifugation. This system is based on electrofiltration where bacterial cell lysates are loaded in one chamber and the purified plasmid DNA is recovered in an adjacent chamber. These two chambers are separated by a membrane made of regenerated cellulose, which allows plasmid DNA to migrate to the recovery chamber while retaining most contaminants in the loading chamber. Unfortunately, even with the optimization of the parameters involved in the electrofiltration, the only DNA that can pass through t

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梁秀敏 and Sau-man Leung. "Direct detection of rifampin-resistant mycobacterium tuberculosis in clinical specimens by DNA sequencing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970102.

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Leung, Sau-man. "Direct detection of rifampin-resistant mycobacterium tuberculosis in clinical specimens by DNA sequencing." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B2329498X.

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Otten, Nathalie. "DNA of bovine papillomavirus type 1 and 2 in equine sarcoids : PCR detection and direct sequencing /." [S.l.] : [s.n.], 1992. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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Rocha, Ana Laís Bignotto da. "Sequenciamento direto dos genes SIX3, SHH, TGIF1, ZIC2 e array-CGH no estudo de pacientes com holoprosencefalia." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/61/61132/tde-12112013-150520/.

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Objetivos: Analisar por meio da técnica de sequenciamento direto a presença de alterações moleculares nos genes SHH, SIX3, ZIC2 e TGIF1 em indivíduos com diagnóstico clínico de HPE. Analisar por meio da técnica de CGH-array a presença de alterações moleculares em indivíduos com diagnóstico clínico de HPE previamente submetidos à análise por sequenciamento direto. Local: Laboratório de Genética e Citogenética Humana HRAC/USP, Bauru-SP. Casuística e metodologia: Foram selecionados 50 indivíduos, de ambos os sexos com idades entre 03 meses a 50 anos com diagnóstico clínico para HPE. Todos foram a

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Vander, Wiede Jamie. "Direct, hands-on or inquiry instruction a study of instructional sequencing and motivation in the science classroom." Master's thesis, University of Central Florida, 2011. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/5073.

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Currently, a debate exists between the strengths and weaknesses of direct and inquiry instruction. Inquiry instruction is related to positive effect on learner motivation whereas supporters of direct instruction point to its ability to adequately support learners' working memories (Hmelo-Silver, Duncan, & Chinn, 2007; Kirschner, Sweller, & Clark, 2006; Kuhn, 2007; Sweller, 1988). This study examined the possibility of combining the best features of both inquiry and direct instruction by sequencing them together. A two-part lesson on electrical circuits was presented in three separate sequences

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7

Koziol, Adam. "Application of Direct-sequencing Peptide Proteomics to the Characterization of Antagonistic (Endogenous and Exogenous) Proteins in Cereal Grains." Thèse, Université d'Ottawa / University of Ottawa, 2013. http://hdl.handle.net/10393/23853.

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The cereal seed plays a crucial role in society – both in the “food as medicine” paradigm, but also in food security. It is the starch and proteins present in the seed that lend it importance in these dissimilar anthropomorphic activities. This thesis investigation first characterized the post-translational processing of the potential diabetogen, wheat globulin-3. Globulin-3-like peptides were observed primarily in the embryo. These peptides varied significantly in their molecular masses and isoelectric points, as determined by two dimensional electrophoresis and immunoblotting. Five major po

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Nguyen, Thuy. "Ultra-deep sequencing applications in virology research." Electronic Thesis or Diss., Sorbonne université, 2018. http://www.theses.fr/2018SORUS282.

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Les deux virus d’ARN VIH et VHC attirent beaucoup d’attention de la santé publique parce qu’ils partagent des mêmes facteurs de risque de transmission par le sang et par des contacts sexuels. En plus, les maladies associées aux infections par le VIH et par le VHC sont des causes principales de mortalité et de morbidité globalement. Néanmoins, grâce à l’introduction des thérapies d’antirétroviraux pour le traitement de l’infection par le VIH et les antiviraux à action directe (AADs) pour le traitement de l’infection par le VHC, les patients infectés par ces virus constatent une amélioration sig

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Engström, Erik. "Direct poly(A) RNA nanopore sequencing on the freshwater duck mussel Anodonta anatina following exposure to copper : A pilot study." Thesis, Högskolan i Skövde, Institutionen för biovetenskap, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:his:diva-17508.

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Aquatic ecotoxicology is the study of toxic chemicals and its effects on aquatic biological systems with the aim of minimising threats to human health and ensure self-sustained ecosystems. Freshwater bivalves are excellent sentinels for use in ecotoxilogical research due to their filter feeding properties, stationary lifestyle and inability to regulate body temperature. This project aimed to assess the feasibility and use of nanopore sequencing, a real-time single-molecule sequencing technology in comparative expression analysis by sequencing transcriptomic RNA from the freshwater mussel Anodo

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Busson, Laurent. "Evolution of direct diagnostic techniques in Virology; analytical performances and clinical input." Doctoral thesis, Universite Libre de Bruxelles, 2020. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/313391.

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Le diagnostic virologique est un sujet d’actualité particulièrement du fait des récentes épidémiesou pandémies telles que la pandémie d’influenza A(H1N1) en 2009 ou la diffusion du virus Zika dansles Amériques et la région du Pacifique entre 2014 et 2017, associée à des cas de microcéphalie et dessyndromes de Guillain Barré. Encore plus récemment, en août 2018, le ministre de la santé de laRépublique Démocratique du Congo annonçait la 10e épidémie de virus Ebola dans le pays et endécembre 2019, le coronavirus SARS-CoV-2 est à l’origine d’une pandémie au départ de la Chine. Avecle nombre croiss

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Takeda, Haruhiko. "Evolution of multi-drug resistant HCV clones from pre-existing resistant-associated variants during direct-acting antiviral therapy determined by third-generation sequencing." Kyoto University, 2018. http://hdl.handle.net/2433/232107.

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Mayrthaler, Laura [Verfasser], and Josef [Akademischer Betreuer] Eberle. "Next Generation Sequencing as screening method for resistance associated variants in Hepatitis C Virus before treatment with direct acting antivirals / Laura Mayrthaler ; Betreuer: Josef Eberle." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1196528918/34.

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RUSSO, C. LO. "ANALISI COMPARATIVA DI METODICHE MOLECOLARI PER LA DETERMINAZIONE DELLO STATO MUTAZIONALE DI EGFR IN PAZIENTI CON CARCINOMA DEL POLMONE NON A PICCOLE CELLULE." Doctoral thesis, Università degli Studi di Milano, 2014. http://hdl.handle.net/2434/231152.

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The epidermal growth factor receptor (EGFR) is a transmembrane receptor with tyrosine-kinase activity that can be activated by different ligands. This event triggers a cascade of signals downstream with effects on cell survival and proliferation. The mutational event of EGFR is able to induce the constitutional activation that can be effectively blocked by the administration of drugs with anti-receptor activity. The evaluation of EGFR mutational profile is a fundamental survey to select patients affected by advanced non-small cell lung cancer (NSCLC), which can undergo treatment with specific

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14

Walbert, Katrin. "Ectomycorrhizal communities associated with a Pinus radiata plantation in the North Island, New Zealand." Lincoln University, 2008. http://hdl.handle.net/10182/658.

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Aboveground and belowground ectomycorrhizal (ECM) communities associated with different age classes of the exotic plantation species Pinus radiata were investigated over the course of two years in the North Island of New Zealand. ECM species were identified with a combined approach of morphological and molecular (restriction fragment length polymorphism (RFLP) and DNA sequencing) analysis. ECM species richness and diversity of a nursery in Rotorua, and stands of different ages (1, 2, 8, 15 and 26 yrs of age at time of final assessment) in Kaingaroa Forest, were assessed above- and belowground;

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Corbin, Alastair Lawrence. "IRF5 directs colonic inflammation and control of mononuclear phagocyte adaptation to the tissue environment." Thesis, University of Oxford, 2017. http://ora.ox.ac.uk/objects/uuid:fb846ef1-e2a4-476f-a8f8-b52ef776ef41.

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Macrophages are leukocytes of the innate immune system that display great phenotypic plasticity to mediate diverse functions. The ontogeny of tissue resident macrophages has been debated in recent decades. It is now recognised that tissue macrophages can be replenished from embryonically-derived precursors, and/or monocyte intermediates in a tissue specific manner. Interferon Regulatory Factor 5 (IRF5) is a transcription factor that promotes a pro-inflammatory phenotype in macrophages in vitro and in vivo. Indeed, IRF5 contributes to the pathogenesis of experimental inflammatory arthritis, lup

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16

Silva, Tiago Roque Benetoli da [UNESP]. "Adubação nitrogenada e resíduos vegetais no desenvolvimento do feijoeiro (Phaseolus vulgaris L.) em sistema de plantio direto." Universidade Estadual Paulista (UNESP), 2002. http://hdl.handle.net/11449/98869.

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Made available in DSpace on 2014-06-11T19:29:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2002-02-15Bitstream added on 2014-06-13T18:39:44Z : No. of bitstreams: 1 silva_trb_me_ilha.pdf: 139678 bytes, checksum: 6795da3822627218071676c7e9fdf22c (MD5)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>A implantação da cultura do feijão no sistema de plantio direto vem crescendo em todo território nacional, entretanto, o plantio direto exige uma adubação nitrogenada diferenciada, tornando-se necessário buscar mais informações a esse respeito. Nesse contexto, o trabalho f

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17

Lima, Filho Diogo Ferreira. "A codificação como proteção contra a interferencia internacional em sistemas de comunicações usando a tecnica de espalhamento espectral do tipo sequencia direta." [s.n.], 1991. http://repositorio.unicamp.br/jspui/handle/REPOSIP/259784.

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Orientador: Reginaldo Palazzo Junior<br>Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Engenharia Eletrica<br>Made available in DSpace on 2018-07-14T04:15:36Z (GMT). No. of bitstreams: 1 LimaFilho_DiogoFerreira_M.pdf: 10803002 bytes, checksum: 974014e1fff2744db1b68cdf6b65db94 (MD5) Previous issue date: 1991<br>Resumo: A combinação das técnicas de espalhamento espectral, entrelaçamento e correção de erros provê aos sistemas de comunicações efetivos ganhos de processamento quando estes mesmos sistemas estão sujeitos à interferência intencional. Fundamentado nesta prem

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Moura, Jaqueline Zanon de [UNESP]. "Distribuição espacial e plano de amostragem sequencial de Pseudoplusia includens (Walker) (Lepidoptera: Noctuidae), em dois sistemas de plantio de soja." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/105130.

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Made available in DSpace on 2014-06-11T19:33:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-08-17Bitstream added on 2014-06-13T19:44:31Z : No. of bitstreams: 1 moura_jz_dr_jabo.pdf: 273227 bytes, checksum: 312d1c99dce447e8d809378e074eb34d (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>O controle de pragas da soja representa até 36,50% do custo de produção. As lagartas desfolhadoras podem comprometer a produção de soja causando desfolhas de até 100%. A dificuldade de controle, associada ao aumento populacional e seriedade dos danos causados colocam

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Roxo, Sónia Catarina Martins. "Integração de informação secundária na modelação geoestatística da qualidade de solos em locais potencialmente contaminados. Aplicação à área subjacente de uma antiga refinaria." Master's thesis, Faculdade de Ciências e Tecnologia, 2011. http://hdl.handle.net/10362/10226.

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Dissertação para obtenção do Grau de Mestre em Engenharia Geológica (Georrecursos)<br>Este trabalho apresenta uma metodologia para caracterizar espacialmente a contaminação em hidrocarbonetos totais do petróleo (Total Petroleum Hydrocarbons - TPH) no local de um antigo parque de combustíveis. A metodologia proposta foi desenvolvida com algoritmos de estimação e simulação geoestatística 3D e integra informação principal e secundária. Foram utilizados dados de 131 sondagens de uma anterior campanha de prospecção, para uma área de aproximadamente 14,5ha. As variáveis secundárias são apresenta

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Quirino, Geise de Aguiar. "Análise de mutações em formas recessivas de pacientes com Asteogênese Inperfeita do Espírito Santo: comparação de metodologias." Universidade Federal do Espírito Santo, 2012. http://repositorio.ufes.br/handle/10/5758.

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Made available in DSpace on 2016-12-23T13:49:05Z (GMT). No. of bitstreams: 1 Geise de Aguiar Quirino.pdf: 839323 bytes, checksum: 5ff1a2735aa2271c126e6d624a6760bd (MD5) Previous issue date: 2012-02-17<br>Osteogenesis Imperfecta (OI) is a genetic desease characterized by patient s bone fragility and deformity, in which severity ranges from a barely detectable connective tissue disorder to lethality in the perinatal period. The diversity of clinical variability in patients is caused by the different location or type of mutations in one of the ten genes related with the disease. This wide cli

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Pereira, Pedro Miguel Martins. "Inversão geoestatística de dados sísmicos 3D com multi-distribuições locais." Master's thesis, Universidade de Aveiro, 2014. http://hdl.handle.net/10773/14410.

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Mestrado em Engenharia Geológica<br>Os modelos de subsuperfície inferidos a partir de dados de reflexão sísmica permitem avaliar espacialmente as propriedades petro-elásticas de reservatório (por exemplo, densidade, impedâncias acústica e elástica) que são essenciais para uma boa caracterização e modelação de reservatórios de hidrocarbonetos. Os modelos invertidos gerados a partir de metodologias geoestatísticas e da integração de dados de logs de poços com dados de reflexão sísmica, dentro de uma grelha de reservatório, permitem ainda avaliar e quantificar, localmente, a incerteza espacial as

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Sukal, Amit Chand. "Molecular characterisation and diagnosis of badnaviruses infecting yams in the South Pacific." Thesis, Queensland University of Technology, 2018. https://eprints.qut.edu.au/122927/1/Amit%20Chand_Sukal_Thesis.pdf.

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Yams are an important food source grown throughout the Pacific but the international movement of the crop is restricted due to a lack of understanding of the viruses infecting these plants. This study focused on identifying and characterising badnaviruses infecting yams and the subsequent development of sensitive and reliable diagnostic tests. These tests can be used in virus-screening programs to enable the safe international movement of virus-free yam germplasm thus contributing to food and nutritional security of Pacific Island Countries.

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Chen, Li-Jung, and 陳麗容. "Breast cancer of Taiwan;p53 gene;SSCP;Direct DNA Sequencing." Thesis, 1995. http://ndltd.ncl.edu.tw/handle/70259252308968030663.

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碩士<br>國立臺灣大學<br>化學研究所<br>84<br>Breast cancer is the most common malignancy cancer of women in Western countries. The mortality rate have held almost steady over the past 20 years, even though the number of new cases has grown. From 1970 to 1990, breast cancer is an important cancer of women in Taiwan and incidence of age in breast cancer trend to early onset. At molecular level, various genetic alteration have been implicated in the development of breast cancer. Loss of heterozygosity(LOH) at five different regions of

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"Direct metagenomic detection and analysis of plant viruses using an unbiased high-throughput sequencing approach." THE UNIVERSITY OF OKLAHOMA, 2009. http://pqdtopen.proquest.com/#viewpdf?dispub=3352842.

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Lin, Yuh-cheng, and 林育成. "Detection of p53 gene mutation in human breast neoplasm with single-strand conformation polymorphism and direct cyclic sequencing." Thesis, 1996. http://ndltd.ncl.edu.tw/handle/36528981266891361444.

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碩士<br>國立成功大學<br>藥理學研究所<br>85<br>p53是一種癌症抑制基因(tumor supressor gene)，在人類常見的癌症當中，可發現p53基因的等位基因缺失(allelic deletion)或點突變(point mutation)所造成的功能喪失。當DNA受到了損傷之後，p53蛋白的量在細胞內急遽增加，隨之使細胞週期停留在G1期；細胞若包含突變的p53或缺乏p53則不能誘導此種細胞週期停止。此外，突變的p53基因使細胞進行計畫性細胞自殺(apoptosis)的能力降低，因此癌細胞內的p53基因若突變則其對化學療法或放射線療法將具有抵抗性。在與其他種致癌基因比較之下，p53基因的突變或缺失是在乳房癌中最常見的基因變化。因此，若能建立p53基因與乳房癌惡化程度之間的關係，將有助於診斷乳房癌或者評估其在治療後復發的可能性。本實驗的目的在於偵測不同病期的乳房癌中其 p53基因突變的情形，藉此來推斷乳房癌的預後(prognosis)。我們首先利

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Lewin, Jörn [Verfasser]. "Method development for quantitative methylation analysis by direct bisulfite sequencing, raw data processing and analysis of the Human Epigenome Project / von Jörn Lewin." 2008. http://d-nb.info/987532677/34.

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TESTI, Silvia. "Dissecting the genetic heterogeneity of familial dementias." Doctoral thesis, 2011. http://hdl.handle.net/11562/349107.

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La demenza è una sindrome clinica caratterizzata da declino cognitivo le cui principali manifestazioni comprendono la perdita di memoria, deficit del linguaggio, deterioramento delle capacità di pianificazione e di giudizio, cambiamenti di comportamento e, non di rado, compromissione delle funzioni motorie ed infine dell'autonomia. Oggigiorno, si stima che circa 36 milioni di persone in tutto il mondo siano affette da demenza, stime che, secondo le ultime predizioni, è destinata a crescere fino a 115 milioni nel 2050. Considerando non solo la prevalenza bensì anche l'impatto sociale ed economi

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Hanzlíčková, Johana. "Zhodnocení kryptické diverzity ve skupině lakušníku niťolistého (Ranunculus trichophyllus agg.)." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-446186.

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Ranunculus trichophyllus agg. (thread-leaved water crowfoot) represents a taxonomically challenging group of aquatic plants in which the presence of several significantly different genotypes and the genome size variation have been recently revealed. The results of previous studies suggest that cryptic taxa occur in this group, being so far overlooked due to considerable morphological reduction and extensivephenotypic plasticity. In this thesis, the variation and genetic relationships of four morphologically similar homophyllous water-crowfoot species was critically assessed in the area of Cent

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"Individualizing the Informed Consent Process for Whole Genome Sequencing: A Patient Directed Approach." Doctoral diss., 2013. http://hdl.handle.net/2286/R.I.20945.

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abstract: ABSTRACT Whole genome sequencing (WGS) and whole exome sequencing (WES) are two comprehensive genomic tests which use next-generation sequencing technology to sequence most of the 3.2 billion base pairs in a human genome (WGS) or many of the estimated 22,000 protein-coding genes in the genome (WES). The promises offered from WGS/WES are: to identify suspected yet unidentified genetic diseases, to characterize the genomic mutations in a tumor to identify targeted therapeutic agents and, to predict future diseases with the hope of promoting disease prevention strategies and/or offering

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Jiang, Jiao. "Use of directed evolution to generate multiple-stress tolerant Oenococcus oeni for enhanced malolactic fermentation." Thesis, 2017. http://hdl.handle.net/2440/114505.

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This study aimed to optimise Oenococcus oeni for more efficient malolactic fermentation in wine with multiple stressors. First, a previously evolved ethanol tolerant strain, A90, was characterised for resistance to combined pH and ethanol stress in both MRSAJ and Red Fermented Chemically Defined Grape Juice Medium (RFCDGJM). A90 showed a similar viability in RFCDGJM compared to its parent, SB3, indicating the need for further improvement. With the success of the previous proof-of-concept directed evolution (DE) in O. oeni, a new DE was carried out to determine 1) if DE can be applied to furth

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Kabahuma, Rosemary I. "Genetic aspects of hearing loss in the Limpopo Province of South Africa." Thesis, 2010. http://hdl.handle.net/10539/8566.

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The aetiological diagnosis of recessive non-syndromic hearing loss poses a challenge owing to marked heterogeneity and the lack of identifying clinical features. The finding that up to 50% of recessive non-syndromal genetic hearing loss among Caucasians was due to mutations in GJB2, the gene encoding Connexin 26 (Cx26) was a breakthrough, whose value as a diagnostic tool has been limited by the significant variation in the prevalence of deafness genes and loci among population groups. The significant association of the GJB6-D13S1830 deletion among individuals with one mutant GJB2 allele

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Ntuli, Zanele Nonhlanhla. "Gender and dramatic discourse with reference to Zakes Mda's selected plays." Diss., 2019. http://hdl.handle.net/10500/26234.

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Text in English, Tswana and siSwati<br>This dissertation examines the multiplicity of social positions within which African women in the postcolonial era find themselves. It focuses on how the dramatic dialogue depicts the positions of women in Zakes Mda’sThe Nun’s Romantic Story, And the Girls in their Sunday Dresses and You Fool, How can the Sky Fall. The study is intended to explore the dramatic dialogue in these plays and to show whether there is any evidence of change in women’s positions. It seeks to demonstrate the extent to which the positions of women have changed and also how

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Correia, João Pedro Pequito. "Contributo para a modelação 3D de horizontes geológicos com o auxílio de sísmica de reflexão e dados de sondagens e poços: um caso de estudo na Bacia Cenozóica do Baixo Tejo." Master's thesis, 2017. http://hdl.handle.net/10362/22223.

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O presente trabalho tem como principal objetivo apresentar e testar uma metodologia que permita a integração de perfis sísmicos de reflexão (informação secundária ou soft) e dados de sondagens e poços (informação principal ou hard) na modelação estocástica da morfologia de horizontes geológicos. Divide-se em duas etapas principais: primeiro são geradas superfícies representativas dos horizontes, só condicionais aos dados da sísmica e, posteriormente, faz-se o condicionamento destas superfícies aos dados de sondagens e poços. Para a geração das superfícies primárias, só condicionais aos dados

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Marques, Inês Alexandra Costa. "Reservatórios turbidíticos – simulação da morfologia por estatísticas multiponto e avaliação das propriedades petrofísicas." Master's thesis, 2015. http://hdl.handle.net/10362/17046.

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Esta dissertação apresenta uma metodologia original para simular a morfologia e as propriedades petrofísicas de reservatórios de hidrocarbonetos em sistemas de canais turbidíticos. Estes sistemas são constituídos por complexos, ou seja, conjuntos de canais de arquitetura meandriforme, e são considerados importantes alvos para a indústria petrolífera. A simulação da morfologia divide-se em duas partes, primeiramente é simulada a trajetória do complexo e depois são simuladas as trajetórias dos canais propriamente ditos condicionadas à trajetória do complexo. O algoritmo de simulação utiliza as

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