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Rozprawy doktorskie na temat „DNA extract”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Hua, Xuequn Helen. "Regulation of initiation of DNA replication in Xenopus egg extract /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1997. http://wwwlib.umi.com/cr/ucsd/fullcit?p9814545.

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Blow, J. J. "The control of eukaryotic DNA replication." Thesis, University of Cambridge, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233674.

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One of the major limitations on research into the control of eukaryotic DNA replication has been the lack of any cell-free system that initiates DNA replication in vitro. The first part of the disseration describes the establishment of a eukaryotic system, derived from the activated eggs of the South African clawed toad, Xenopus laevis, that efficiently initiates and completes DNA replication in vitro. Using a variety of biochemical techniques I show that DNA added to the extract in the form of sperm nuclei is efficiently replicated over a period of 4 - 6 hours. Replication of nuclear DNA repr

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Tendwa, Maureen Bilinga. "Investigating effects of aqueous root extract of Mondia whitei on sperm functionality." University of the Western Cape, 2016. http://hdl.handle.net/11394/5547.

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Magister Scientiae (Medical Bioscience) - MSc(MBS)<br>Introduction: Mondia whitei commonly known as "White Ginger" is a highly acclaimed medicinal plant that is extensively used across Africa. M. whitei is used as treatment for sexual dysfunction and is considered to be an aphrodisiac by traditional medicine practitioners. Yet, scientific evidence to support these claims are minimal and those that are published possess ambiguity. To date, only one study reporting the in vitro effect of the aqueous rhizome extract of M. whitei on human sperm motility is available. Therefore, the aim of the stud

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Slenn, Tamara Jeannine. "The Ubiquitin Ligase \(CRL4^{Cdt2}\) Targets Thymine DNA Glycosylase for Destruction during DNA Replication and Repair." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:10839.

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The E3 ubiquitin ligase \(CRL4^{Cdt2}\) targets proteins for destruction during DNA replication and following DNA damage (Havens and Walter, 2011). Its substrates contain "PIP degrons" that mediate substrate binding to the processivity factor PCNA at replication forks and damage sites. The resulting PCNA-PIP degron complex forms a docking site for \(CRL4^{Cdt2}\), which ubiquitylates the substrate on chromatin. Several \(CRL4^{Cdt2}\) substrates are known, including Cdt1, multiple CDK inhibitors, Drosophila E2f1, human Set8, S. pombe Spd1, and C. elegans \(Pol\eta\) (Havens and Walter, 2011).

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Kransdorf, Evan Paul. "Purification and Characterization of a Methyl-DNA Binding Protein Complex from Primary Erythroid Cells." VCU Scholars Compass, 2004. http://hdl.handle.net/10156/1685.

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Erasmus, Nicolete. "Investigations on the in vitro effects of aqueous Eurycoma longifolia Jack extract on male reproductive functions." Thesis, University of Western Cape, 2012. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_2238_1375971626.

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<p>Eurycoma longifolia (Tongkat Ali<br>TA) is a Malaysian shrub used to treat various illnesses including male infertility. Considering that TA is also used to improve male fertility and no report&nbsp<br>regarding its safety has been published, this study investigated the effects of a patented, aqueous TA extract on various sperm and testicular functions. Materials and Methods This study&nbsp<br>encompasses two parts (part 1: on spermatozoa<br>part 2: on TM3-Leydig and TM4-Sertoli cells). Part 1: Semen samples of 27 patients and 13 fertile donors were divided into two groups,&nbsp<br>washed a

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Shrestha, Prashanta. "Streamlined Extract Preparation for E. coli-Based Cell-Free Protein Synthesis and Rapid Site-Specific Incorporation of Unnatural Amino Acids in Proteins." BYU ScholarsArchive, 2012. https://scholarsarchive.byu.edu/etd/3917.

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This thesis reports the viability of E. coli cell extracts prepared using equipment that is both common to biotechnology laboratories and able to process small volume samples and expression of proteins containing unnatural amino acids (UAAs) at higher level using PCR amplified linear DNA templates (LETs) in cell-free protein synthesis (CFPS) system. E. coli-based cell extracts are a vital component of inexpensive and high-yielding CFPS reactions. However, effective preparation of E. coli cell extract is limited to high-pressure homogenizers (French press style or impinge-style) or bead mill ho

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Romanowski, Piotr. "Cell cycle regulation of DNA replication in Xenopus egg extracts." Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.624760.

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Sun, Li. "Studies of initiation of DNA replication using xenopus egg extracts /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2000. http://wwwlib.umi.com/cr/ucsd/fullcit?p9989761.

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Mason, Rebecca Mary Aglaia. "The rejoining of non-homologous DNA double-strand breaks by mammalian cell free extracts." Thesis, University of Sussex, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.336323.

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Pung, Thitiya. "The Isolation of Natural Products From Plant Extracts." Thesis, Virginia Tech, 2000. http://hdl.handle.net/10919/33973.

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Bioassay-guided fractionation of the ethyl acetate extract of Virola sp. (Myristicaceae) using the Sc7 yeast strain resulted in the isolation of the weakly cytotoxic biochanin A (an isoflavone compound). The bioassay of three mutant yeast strains (1138, 1104, and 1353) directed the isolation of DNA-damaging alkaloids from Solanum hostmannii. These alkaloids were identified as verazine and (20R) epimer verazine. In addition, three oxoaporphine alkaloids were isolated from the bark of Papualthia sp. (Annonaceae). Oxocrebanine and atherospermidine showed DNA-damaging activity whereas liriod

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Clark, Joanna Rebecca. "The detection of DNA-binding proteins in nuclear extracts of barley endosperm." Thesis, Imperial College London, 1990. http://hdl.handle.net/10044/1/47812.

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Byrne, Shaun Edward. "An investigation into the processing of ionising radiation induced clustered DNA damage sites using mammalian cell extracts." Thesis, University of Oxford, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670082.

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Dean, Kristina. "Degradability of both a physical latent fingerprint and its associated extracted DNA." [Cedar City, Utah] : Southern Utah University, 2009. http://unicorn.li.suu.edu/ScholarArchive/ForensicScience/DeanKristina.pdf.

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Sun, Wei-Hsin. "The induction of DNA replication in quiescent mammalian nuclei by Xenopus egg extracts." Thesis, King's College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314371.

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Adjerid, Nassiba. "The Dynamics of the Unreplicated DNA Checkpoint in Xenopus laevis Embryos and Extracts." Diss., Virginia Tech, 2008. http://hdl.handle.net/10919/26681.

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When unreplicated or damaged DNA is present, cell cycle checkpoint pathways cause cell cycle arrest by inhibiting cyclin-dependent kinases (Cdks). In Xenopus laevis, early embryonic development consists of twelve rapid cleavage cycles between DNA replication (S) and mitosis (M) without checkpoints or gap phases. However, checkpoints are engaged in Xenopus once the embryo reaches the midblastula transition (MBT). At this point, the embryo initiates transcription, acquires gap phases between S and M phases, and establishes a functional apoptotic program. During the cell cycle, there are two mai

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17

Iyavoo, Sasitaran. "Development and application of a PCR multiplex to assess the quality and quantity of forensic DNA extracts." Thesis, University of Central Lancashire, 2014. http://clok.uclan.ac.uk/11317/.

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Isolation of DNA from skeletonised human remains can be problematic. In addition to DNA degradation, enhanced by high temperature and humidity, there are often potent polymerase chain reaction (PCR) inhibitors present within the samples. It is therefore important to extract the maximum amount of available DNA whilst removing any amplification inhibitors that may be present. Whilst real-time PCR methods are available for quantification and detection of PCR inhibitors the information received is limited as real-time PCR targets amplicons that are much smaller than those typically targeted in for

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Gonçalves, Álvaro Luiz Martini [UNESP]. "Investigação do potencial genótóxico e antigenotóxico do extrato de Brassica oleracea in vivo." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/87927.

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Made available in DSpace on 2014-06-11T19:23:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-02-27Bitstream added on 2014-06-13T18:09:12Z : No. of bitstreams: 1 goncalves_alm_me_botib.pdf: 590776 bytes, checksum: 8b55e04bfd5d2925a1cf2b9ac4822357 (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Brassica oleracea é uma espécie botânica popularmente conhecida como “couve”. É utilizada na alimentação e também na medicina popular, devido às suas ações antiulcerogênica e quimiopreventiva do câncer, entre outras. Este estudo teve como objetivo avaliar o pote

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Gonçalves, Álvaro Luiz Martini. "Investigação do potencial genótóxico e antigenotóxico do extrato de Brassica oleracea in vivo /." Botucatu, 2012. http://hdl.handle.net/11449/87927.

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Orientador: Edson Luis Maistro<br>Banca: Mária Sérgio Mantovani<br>Banca: Wilma de Grava Kempinas<br>Resumo: Brassica oleracea é uma espécie botânica popularmente conhecida como "couve". É utilizada na alimentação e também na medicina popular, devido às suas ações antiulcerogênica e quimiopreventiva do câncer, entre outras. Este estudo teve como objetivo avaliar o potencial genotóxico e antigenotóxico do extrato de folhas de Brassica oleracea em células somáticas e germinativas de camundongos in vivo, usando o Ensaio Cometa e o teste do micronúcleo. Os animais (camundongos Swiss albinos machos

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20

Badhe, Pravin. "Characterisation of fractions from Andrographis paniculata and Silybum marianum plant extracts that protect human cells against DNA damage." Thesis, Brunel University, 2016. http://bura.brunel.ac.uk/handle/2438/14796.

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Plants have been utilized as a source of medicines since ancient times. They contain a vast range of secondary metabolites which play important roles in different diseases. The Scope of this study is to define the function of secondary metabolites from Andrographis paniculata (Kalmegh) and Silybum marianum (Milk Thistle) against DNA damage, which initiates many diseases. Sequential extraction of both plant materials was performed with different polarity solvents. Qualitative analysis was performed with Gas (GC) and High Performance Liquid Chromatography (HPLC). Primary extracts screening studi

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Pereira, Luciana Oliveira. "Apoptose induzida por extrato aquoso de Pteridium aquilinum em células de glândula submandibular humana (HSG) e de epitélio bucal (OSCC-3)." reponame:Repositório Institucional da UnB, 2006. http://repositorio.unb.br/handle/10482/5775.

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Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Biológicas, Departamento de Biologia Celular, 2006.<br>Submitted by Fernanda Weschenfelder (nandaweschenfelder@gmail.com) on 2009-11-06T13:22:40Z No. of bitstreams: 1 Dissertacao Luciana Oliveira Pereira.pdf: 3419957 bytes, checksum: 43ae3c1efe69a8a2bbcf0d6b0b0d96bd (MD5)<br>Approved for entry into archive by Gomes Neide(nagomes2005@gmail.com) on 2010-10-27T13:48:50Z (GMT) No. of bitstreams: 1 Dissertacao Luciana Oliveira Pereira.pdf: 3419957 bytes, checksum: 43ae3c1efe69a8a2bbcf0d6b0b0d96bd (MD5)<br>Made available in D

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22

Khairat, Ibrahim Abdelhay Rabab [Verfasser], and Nikolaus [Akademischer Betreuer] Blin. "Next generation sequencing of DNA extracted from mummified tissue / Rabab Khairat Ibrahim Abdelhay ; Betreuer: Nikolaus Blin." Tübingen : Universitätsbibliothek Tübingen, 2013. http://d-nb.info/1162844647/34.

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Prieto, Aline Miranda [UNESP]. "Avaliação de Reparo de DNA e Atividade Antioxidante do Extrato Etanólico e Substâncias Isoladas de Casearia sylvestris." Universidade Estadual Paulista (UNESP), 2012. http://hdl.handle.net/11449/100124.

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Made available in DSpace on 2014-06-11T19:30:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-11-28Bitstream added on 2014-06-13T18:40:38Z : No. of bitstreams: 1 prieto_am_dr_arafcf.pdf: 2994693 bytes, checksum: 4a5745efb3d1e4179f19e828a3811dc8 (MD5)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>Universidade Estadual Paulista (UNESP)<br>Trabalhos demonstraram que derivados da planta Casearia sylvestris protegem o DNA de danos. Dessa forma, este trabalho visa avaliar por quais mecanismos essa proteção ocorre. Os produtos naturais avaliados foram o extrato etanól

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Prieto, Aline Miranda. "Avaliação de Reparo de DNA e Atividade Antioxidante do Extrato Etanólico e Substâncias Isoladas de Casearia sylvestris /." Araraquara : [s.n.], 2012. http://hdl.handle.net/11449/100124.

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Orientador: Christiane Pienna Soares<br>Coorientador: Eliana Aparecida Varanda<br>Coorientador: Valéria Valente<br>Banca: Denise Crispim Tavares<br>Banca: Raquel Alves dos Santos<br>Banca: André Gonzaga dos Santos<br>Banca: Raquel Mantuaneli Scarel Caminaga<br>Resumo: Trabalhos demonstraram que derivados da planta Casearia sylvestris protegem o DNA de danos. Dessa forma, este trabalho visa avaliar por quais mecanismos essa proteção ocorre. Os produtos naturais avaliados foram o extrato etanólico das folhas de C. sylvestris e os diterpenos clerodânicos obtidos deste extrato: casearinas B, D e X

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Dravid, Amit. "A model of the checkpoint response of the cell cycle of frog-egg extracts in the presence of unreplicated DNA." Thesis, Virginia Tech, 2004. http://hdl.handle.net/10919/36191.

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The cell cycle of eukaryotes consists of alternation between growth and DNA replication (interphase), and DNA distribution and cell-division (mitosis or M-phase). This process is regulated by a complex network of biochemical reactions. A core part of this network, called the "Cell Cycle engine" is evolutionarily conserved. The dimer of CDK1 (a protein kinase) and Cyclin proteins (the regulatory components), called M-phase Promoting Factor (MPF), and its key regulatory proteins Cdc25 and Wee1, are central parts of this cell cycle engine. Maintaining the fidelity of the DNA during the cell cycle

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Silva, Vítor Renato Carvalho e. "Avaliação do potencial citotóxico, genotóxico e clastogênico do extrato aquoso das folhas de arrabidaea chica verlot (bignoniaceae)." Universidade Federal do Amazonas, 2011. http://tede.ufam.edu.br/handle/tede/2557.

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Made available in DSpace on 2015-04-11T13:54:21Z (GMT). No. of bitstreams: 1 vitor renato.pdf: 768606 bytes, checksum: adbc3adac1d4f5dee180dddd0e941803 (MD5) Previous issue date: 2011-02-28<br>CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Crajiru is a shrub of the family Bignoniaceae found throughout the Amazon. Its scientific name is Arrabidaea chica B. Verl. and is popularly known as carajuru, pariri, cipó cruz, calajouru, karajurana, krawiru. It has anti-inflammatory, healing, anti-anemic, and anti-hemorrhagic properties. The variety used was the AC1, for being

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King, Maureen McGregor. "The detection of macromolecular adducts in DNA extracted from animal and human tissue using '3'2p postlabelling and immunassay." Thesis, University of York, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.306311.

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Assumpção, João Henrique Maia. "Potencial efeito epigenético do extrato de própolis brasileira e de seus componentes sobre linhagens celulares derivadas de carcinomas mamários." Botucatu, 2017. http://hdl.handle.net/11449/152409.

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Orientador: Cláudia Aparecida Rainho<br>Resumo: A própolis é uma substância de origem natural, de aspecto resinoso, produzida pelas abelhas a partir de diferentes plantas, que determina a sua composição química. Centenas de componentes foram identificados em diferentes amostras de própolis, cujo extrato exerce uma diversidade de efeitos biológicos e farmacológicos. Alguns estudos demonstraram que este produto pode interferir em vias de sinalização de oncogenes, inibir o crescimento, reduzir a proliferação celular, induzir apoptose e apresentar efeito antiangiogênico. O câncer de mama é uma doe

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ARA?JO, Elisangela Sousa de. "Caracteriza??o molecular (RAPD) e an?lise das prote?nas de reserva em gr?os de variedades locais de arroz do Maranh?o." Universidade Federal Rural do Rio de Janeiro, 2006. https://tede.ufrrj.br/jspui/handle/jspui/1235.

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Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2016-10-04T19:57:29Z No. of bitstreams: 1 2006 - Elisangela Sousa de Araujo.pdf: 1061759 bytes, checksum: 865d0167431190cf5627ccdf21cf91fb (MD5)<br>Made available in DSpace on 2016-10-04T19:57:29Z (GMT). No. of bitstreams: 1 2006 - Elisangela Sousa de Araujo.pdf: 1061759 bytes, checksum: 865d0167431190cf5627ccdf21cf91fb (MD5) Previous issue date: 2006-02-23<br>FAPERJ<br>CAPES<br>Brazil is the country with the greatest plant genetic diversity in the world with the potential still underestimated what makes it necessary to characterize loc

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30

Chvalkovská, Eva. "Využití různých metod izolace DNA baktérií mléčného kvašení v molekulárně biologických metodách." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2019. http://www.nusl.cz/ntk/nusl-401889.

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This thesis focused on the probiotic bacteria, DNA isolated from these bacteria by three different methods and the effect of isolation on DNA identification using molecular biological methods. Probiotic bacteria are an important part of human intestinal tract. They have an important role in the function of the immune system due to adhesion to the mucosa of the intestinal flora. They create a inhostile environment for pathogens. Probiotic bacteria are commonly taken in the food like dairy products or food supplements. However, overuse of antibiotics is at risk of passing on the intrinsic resist

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31

Atnip, Allison A. "Oxidative Stabilities of Docosahexaenoic Acid Oil and Linoleic Acid in an Aqueous System." The Ohio State University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=osu1284727595.

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32

Byrne, Hilary Louisa. "Radiation Interactions In Biological Matter - The Physical Roots of Biological Response to Radiation." Thesis, The University of Sydney, 2017. http://hdl.handle.net/2123/16907.

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Radiotherapy is a widely used therapeutic tool. Development of new techniques in radiotherapy rely on understanding the initial physical interactions of incident particles in biological matter. The processes that lead to energy deposition and so biological damage need to be understood on a range of scales from bulk energy deposition at the centimetre scale down to the details of ionisation distributions at the nanoscale. Monte Carlo (MC) simulation gives insights into these processes that are otherwise difficult to obtain and that can be used to build models of the fundamental physical mechani

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33

Degenhardt, Sarah [Verfasser], and Jochen [Akademischer Betreuer] Dahm-Daphi. "In vitro DNA-Doppelstrangbruch-Reparatur in humanen Kopf-,Hals-Tumorzelllinien : zellfreie Extrakte zur Prädiktion der Strahlenempfindlichkeit / Sarah Degenhardt. Betreuer: Jochen Dahm-Daphi." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2013. http://d-nb.info/1032312904/34.

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Low, Eva Oi Wha. "p53 mutations in cultured malignant cells and oral cancers detected in extracted DNA and in situ, investigated by the polymerase chain reaction." Thesis, The University of Sydney, 2003. http://hdl.handle.net/2123/4646.

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Colombo, Natália Beatriz Rigoldi. "Ação dos compostos antioxidantes na redução do estresse oxidativo em modelo experimental de câncer de pulmão: estudo do pequi (Caryocar brasilense camb)." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-27082014-092810/.

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Introdução. Uma alimentação rica em antioxidantes pode prevenir e reparar danos oxidativos causados pelas espécies reativas de nitrogênio e oxigênio, como o dano no DNA e a peroxidação lipídica e pode reduzir o risco de câncer, aterosclerose e outras doenças degenerativas. A polpa do Caryocar Brasiliense Camb, mais conhecido como pequi, é uma fruta do cerrado brasileiro que possui altos níveis de antioxidantes como os carotenóides, vitamina C e E e compostos fenólicos. Objetivos. Verificar a atividade antioxidante do óleo e do extrato da polpa do pequi na diminuição do estresse oxidativo em um

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36

Jenčo, Michal. "Bioinformatický nástroj pro anotaci transposonů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2017. http://www.nusl.cz/ntk/nusl-363823.

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This thesis provides theoretical resources for the design of a new bioinformatics tool for transposon annotation with focus on their additional structural elements. There is a biological description of transposons, the mobile elements in DNA, their classification and structure. It further deals with the overview and classification of available transposon identification and annotation bioinformatics tools, description of function and implementation of a select few. Next we state the scheme of a new bioinformatics tool for LTR retrotransposon identification and annotation with a focus on extra O

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Syslová, Ivona. "Izolace nukleových kyselin pro diagnostické účely s využitím polymerních nosičů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216492.

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The isolation of deoxyribonucleic acid (DNA) was studied in the diploma thesis by using three different methods: phenol extraction, salting with sodium chloride and magnetic separation with reversible adsorption of nucleic acids on different magnetic carriers. There were used five different properly functionalized carriers for the isolation of DNA: magnetic silicagel, P(HEMA-co-GMA) ox. I, P(HEMA-co-GMA) ox. II, Dynal DNA Direct and Perovskit 439. The reversible imobilization of DNA on the magnetic carrier was proceeded under the conditions of high concentration of NaCl and poly(ethyleneglycol

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38

Presta, Giuseppe Ant?nio. "Efeito de um extrato de chrysobalanus icaco na marca??o de constitu?ntes sangu?neos com tecn?cio-99m, na morfologia de hem?cias, na topologia plasmidial e na a??o do cloreto estanoso no dna plasmidial." Universidade Federal do Rio Grande do Norte, 2007. http://repositorio.ufrn.br:8080/jspui/handle/123456789/13119.

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Made available in DSpace on 2014-12-17T14:13:22Z (GMT). No. of bitstreams: 1 GiuseppeAP.pdf: 289778 bytes, checksum: 0019c3f931b948d4ac6b9aa32d20ec37 (MD5) Previous issue date: 2007-08-16<br>The use of radionuclides has contributed for advances in Health Sciences, to research or to the diagnosis and/or treatment of diseases. These advances have been possible with the utilization of radiopharmaceuticals labeled with technetium-99m (99mTc). Stannous chloride (SnCl2) has the main reducing agent utilized to obtain radiopharmaceuticals labeled with technetium-99m. It has been reported

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Miquel, Fabre Françoise. "Propriétés érythroagglutinantes et mitogéniques d'une lectine (DLA) extraite de Dolichos Lablab L. Caractérisation de ses récepteurs membranaires impliqués dans la stimulation lymphocytaire." Montpellier 1, 1989. http://www.theses.fr/1989MON13504.

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Sabha, Dina Talat Tawfiq [Verfasser], Stefan [Akademischer Betreuer] Dhein, Friedrich-Wilhelm [Gutachter] Mohr, and Michael [Gutachter] Schäfer. "Pharmaceutical and chemical analysis of the components carrying the antiplatelet activity of extracts from allium ursinum and allium sativum / Dina Talat Tawfiq Sabha ; Gutachter: Friedrich-Wilhelm Mohr, Michael Schäfer ; Betreuer: Stefan Dhein." Leipzig : Universitätsbibliothek Leipzig, 2012. http://d-nb.info/1238020666/34.

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Kmeť, Miroslav. "Lokalizace metylačních míst transposonů." Master's thesis, Vysoké učení technické v Brně. Fakulta informačních technologií, 2015. http://www.nusl.cz/ntk/nusl-235006.

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This master's thesis deals with the creation of a tool for the extraction of methylation level from transposon sequences. Transposons are DNA elements with ability to move or copy themselves and their activity is regulated by DNA methylation. Sequence methylation information is stored in the bisulfite data and their processing is done with parts of two existing tools in a combination with implemented modules. Created tool takes into consideration unique challenges brought in the methylation calling process by transposable elements and it's functionality is presented on a set of experiments wit

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François, Céline. "De la recherche fondamentale (Na,K-ATPase et DHA) jusqu'aux essais cliniques en passant par les plantes, (extrait de Desmodium adscendens, infusion de plantes) dans le cadre de la réglementation européenne du complément alimentaire." Poitiers, 2011. http://www.theses.fr/2011POIT1405.

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La Na+,K+-ATPase, ou pompe à sodium, est une enzyme membranaire ubiquitaire permettant le maintien des gradients sodique et potassique indispensables à la survie cellulaire. La première partie de ces travaux a été consacrée à l'étude de l'implication de cette pompe en tant que marqueur de la physiopathologie. Dans ce but, un modèle de section médullaire totale suivie d'une injection de GPL-DHA (glycerophospholipids enriched in docosahexaenoic acid) a été mis en place chez le rat. L'évaluation de l'activité de la Na+,K+-ATPase et la caractérisation de l'expression des isoformes α1, α2 et ß1 au

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Goossens, Benoît. "Système de reproduction et variabilité génétique intra- et interpopulationnelle chez la marmotte alpine (Marmota marmota L. , Sciuridé)." Université Joseph Fourier (Grenoble ; 1971-2015), 1998. http://www.theses.fr/1998GRE10034.

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La marmotte alpine (marmota marmota, sciuride), espece hautement sociale au systeme d'appariement decrit comme etant monogame, suscite un grand interet comme modele d'etude de la socialite chez les mammiferes. Elle forme des unites familiales composees d'un couple dominant et des jeunes issus de portees successives. Apres une etude de faisabilite, l'utilisation des poils comme materiel biologique et des microsatellites comme outil genetique a permis d'etudier le systeme d'appariement de la marmotte alpine en quantifiant les paternites extra-couples dans les groupes familiaux d'une population d

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Greer, Mary Ruth. "PART 1: Screening of Thirty-one Medicinal Plant Species Against Herpes Simplex Virus, Acetone and Methanol Extracts from the Root Tissue of Kalanchoe pinnata Interferes with HSV Types 1 and 2 DNA replication and Early and Late Gene Expression Preventing the Spread of HSV in vitro. PART 2: Professional Development Curriculum: Integrating Molecular Biology and Microbiology into the Existing Secondary Biology Curricula." BYU ScholarsArchive, 2009. https://scholarsarchive.byu.edu/etd/6160.

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PART 1: Thirty-one medicinal plant species from Hawaii, Morocco, and the Sonoran Desert, USA have been shown in past studies to be highly inhibitory to pathogenic bacteria, fungi, and certain cancer cell lines. However, none were tested for antiviral activity. Acetone and methanol extracts from these species were bio-assayed for antiviral activity against herpes simplex virus types 1 and 2 (HSV 1 and HSV 2) and for cytotoxicity to the Vero C1008 cell line. Extracts from these species were tested in vitro for antiviral activity using an immunoperoxidase mini-plaque reduction assay to detect vir

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Ferhat, Fouad. "Une analyse économique de la qualité et de l'efficience des universités et des systèmes universitaires : une comparaison au niveau international." Thesis, Paris 1, 2016. http://www.theses.fr/2016PA01E040/document.

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Cette thèse propose une analyse économique de la qualité et de l'efficience des universités et des systèmes universitaires au niveau international, à travers l'utilisation des indicateurs intrants et extrants et la méthode d'enveloppement des données (Data Envelopment Analysis DEA). La thèse est composée de quatre chapitres. Le premier chapitre intitulé "les classements des universités : une mise en perspective critique" présente et évalue la pertinence des indicateurs intrants/extrants les plus utilisés par les classements des universités. Il est l'occasion d'exposer un certain nombre de crit

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Iglésias, Leonor Vital. "Comparação entre técnicas cirúrgicas para a correcção da ruptura do ligamento cruzado cranial em canídeos." Bachelor's thesis, Universidade Técnica de Lisboa. Faculdade de Medicina Veterinária, 2009. http://hdl.handle.net/10400.5/1508.

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Dissertação de Mestrado Integrado em Medicina Veterinária<br>O objectivo do presente trabalho centra-se na comparação entre técnicas cirúrgicas para a resolução da ruptura do ligamento cruzado cranial em canídeos. Foi baseado numa pesquisa bibliográfica e num estudo estatístico retrospectivo de 11 casos intervencionados no Hospital Escolar, seguidos durante o estágio curricular. Nesse estudo foram incluídos três grupos de técnicas: tibial tuberosity advancement (TTA), técnicas extra-capsulares e técnicas intracapsulares. As técnicas intra-capsulares visam essencialmente substituir o ligam

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Yang, Chih-Feng, and 楊智鋒. "Onion DNA Extract Application By LabCD." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/6m88eh.

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碩士<br>國立臺北科技大學<br>製造科技研究所<br>96<br>Micro-fluid techniques are developed rapidly in the bio-chip area. The Micro Total Analysis System provides the capabilities in sampling, transporting, mixing, separating, and sensing function. This thesis develops four kinds of bio-discs, first red black ink mixing the disc, second red black blue ink mixing the disc, third onion DNA disc is used red black blue ink mixing the simulation disc, fourth onion DNA disc uses the real liquid disc.The critical rotational speed formulae derived from NTFL laboratory, basic rotational speed and application disc two expe

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Huang, Yi-Shie, and 黃以馨. "The role of nitric oxide in areca nut extract-induced DNA damage." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/60006716118185337333.

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Shih, Shang-Lung, and 施尚龍. "Application of comet-nuclear extract assay in studying repair of UVC-induced DNA damages." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/83747096287926297427.

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碩士<br>國立清華大學<br>分子醫學研究所<br>95<br>Comet assay, also called single cell gel electrophoresis (SCGE), is widely used to investigate genotoxin, and DNA repair system. UVC induced DNA adducts were cyclobutane pyrimidine dimmers (CPD) and 6-4 pyrimidine pyrimidone photoproducts (6-4PP). These adducts do not caused DNA breaks immediately, the DNA breaks were the intermediate of repair process that means conventional comet assay can’t estimate total amounts of UVC induced DNA adducts. To overcome this problem, people who demonstrated that damaged DNA was incubate with nuclear extract (NE) or T4 endonuc

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Chien-Wei and 黃千維. "The Differential Effects of Sodium Arsenite, Cigarette Smoke Extract, and Methylglyoxal on Oxidative DNA Damage." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/11753347551063896869.

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碩士<br>中山醫學大學<br>生物醫學科學學系碩士班<br>99<br>OGG1 is the main base excision repair protein that removes 8-oxodG from DNA. Previous studies have shown that sodium arsenite (As3+), cigarette smoke extract (CSE), and methylglyoxal (MG) can significantly induced DNA damage, including 8-oxodG in different human cell lines. And, the intracellular reactive oxygen species (ROS) generation is mainly responsible for their induction of 8-oxodG. However, the suppression of OGG1 expression or activity by As3+, CSE and MG treatments are still unknown. Therefore, the purpose of this study was to determine the possib

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