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Artykuły w czasopismach na temat „Electron microscopy Methodology”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 3 lutego 2022

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1

Iha, Luiz Cesar Nakao, Andre Aguillera, Flavio Faria, Cristiane Akemi Kasse, Oswaldo Laercio Mendonça Cruz, and Edna Frey Muller. "R147: Scanning Electron Microscopy: Alternatives in Methodology." Otolaryngology–Head and Neck Surgery 135, no. 2_suppl (August 2006): P156. http://dx.doi.org/10.1016/j.otohns.2006.06.901.

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Downing, Kenneth H. "Instrumentation and methodology for electron crystallography." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 420–21. http://dx.doi.org/10.1017/s0424820100086404.

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The determination of three-dimensional structures of macromolecules at atomic resolution remains one oμf the great promises of electron microscopy. Instrumental problems which had to be overcome in order to achieve single-atom resolution have long been recognized and to a large extent overcome. It is interesting to note that most of the instrumental developments were already incorporated in a microscope under construction over 20 years ago. However, the application of techniques to circumvent limitations imposed by the sensitivity of organic specimen to radiation damage are still developing. E

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Liang, Alice F., Chris Petzold, Kristen Dancel-Manning, Yan Deng, and Michael Cammer. "Methodology Development at NYULMC Microscopy Core - Correlative Light and Electron Microscopy Applications." Microscopy and Microanalysis 21, S3 (August 2015): 879–80. http://dx.doi.org/10.1017/s143192761500519x.

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Leppard, GG, A. Heissenberger, and GJ Herndl. "Ultrastructure of marine snow. I. Transmission electron microscopy methodology." Marine Ecology Progress Series 135 (1996): 289–98. http://dx.doi.org/10.3354/meps135289.

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Danev, Radostin, Haruaki Yanagisawa, and Masahide Kikkawa. "Cryo-Electron Microscopy Methodology: Current Aspects and Future Directions." Trends in Biochemical Sciences 44, no. 10 (October 2019): 837–48. http://dx.doi.org/10.1016/j.tibs.2019.04.008.

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Sicignano, A., and M. Vaez Iravani. "Methodology and practice of in situ differential scanning electron microscopy." Scanning 12, no. 2 (1990): 61–68. http://dx.doi.org/10.1002/sca.4950120203.

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Asher, Lucas, and Jessica Hata. "Platelet Electron Microscopy: Utilizing LEAN Methodology to Optimize Laboratory Workflow." Pediatric and Developmental Pathology 23, no. 5 (May 19, 2020): 356–61. http://dx.doi.org/10.1177/1093526620915361.

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Background Platelet electron microscopy (PEM) is the gold standard methodology for diagnosing storage pool disorder, defined as a paucity of delta granules, alpha granules, or both. PEM literature is limited with few published resources and without well-developed interlaboratory standardization for the preparation and examination of platelet samples. Methods Whole mount (WM) dense body (DB) counts for 300 pediatric cases were reviewed to determine whether counting fewer platelets could yield the same results. For 6 cases, DB average was determined on the day of WM preparation and on 2 consecut

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Kammers, A. D., and S. Daly. "Digital Image Correlation under Scanning Electron Microscopy: Methodology and Validation." Experimental Mechanics 53, no. 9 (July 11, 2013): 1743–61. http://dx.doi.org/10.1007/s11340-013-9782-x.

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Menteş, T. O., G. Zamborlini, A. Sala, and A. Locatelli. "Cathode lens spectromicroscopy: methodology and applications." Beilstein Journal of Nanotechnology 5 (October 27, 2014): 1873–86. http://dx.doi.org/10.3762/bjnano.5.198.

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The implementation of imaging techniques with low-energy electrons at synchrotron laboratories allowed for significant advancement in the field of spectromicroscopy. The spectroscopic photoemission and low energy electron microscope, SPELEEM, is a notable example. We summarize the multitechnique capabilities of the SPELEEM instrument, reporting on the instrumental aspects and the latest developments on the technical side. We briefly review applications, which are grouped into two main scientific fields. The first one covers different aspects of graphene physics. In particular, we highlight the

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Yang, Jinfeng, and Yoichi Yoshida. "Relativistic Ultrafast Electron Microscopy: Single-Shot Diffraction Imaging with Femtosecond Electron Pulses." Advances in Condensed Matter Physics 2019 (May 2, 2019): 1–6. http://dx.doi.org/10.1155/2019/9739241.

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We report on a single-shot diffraction imaging methodology using relativistic femtosecond electron pulses generated by a radio-frequency acceleration-based photoemission gun. The electron pulses exhibit excellent characteristics, including a root-mean-square (rms) illumination convergence of 31 ± 2 μrad, a spatial coherence length of 5.6 ± 0.4 nm, and a pulse duration of approximately 100 fs with (6.3 ± 0.6) × 106 electrons per pulse at 3.1 MeV energy. These pulses facilitate high-quality diffraction images of gold single crystals with a single shot. The rms spot width of the diffracted beams

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Chudnovsky, A., K. Sehanobish, and S. Wu. "Methodology for Durability Analysis of HDPE Pipe." Journal of Pressure Vessel Technology 122, no. 2 (December 15, 1999): 152–55. http://dx.doi.org/10.1115/1.556165.

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Toughness evaluation and durability analysis are two of the critical steps to design a toughened HDPE resin for durability in pipe applications. Durability analysis involves defect characterization, crack initiation and propagation mechanism, and long-term performance prediction. The methodology for durability analysis of high-density polyethylene (HDPE) pipe will be discussed in this paper. Various analytical techniques, such as fractography, hot-stage microscopy, energy-dispersive X-ray (EDX), microtransmittance infrared spectroscopy, scanning electron microscopy (SEM), and transmission elec

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12

Kaushik, Madhu, Wei C. Chen, Theo G. M. van de Ven, and Audrey Moores. "An improved methodology for imaging cellulose nanocrystals by transmission electron microscopy." Nordic Pulp & Paper Research Journal 29, no. 1 (January 1, 2014): 77–84. http://dx.doi.org/10.3183/npprj-2014-29-01-p077-084.

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Smith, Paul, John Gannon, and Frank Eggert. "A New Methodology for Element Imaging in the Scanning Electron Microscope." Microscopy and Microanalysis 7, S2 (August 2001): 1054–55. http://dx.doi.org/10.1017/s1431927600031342.

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The analysis of samples with a scanning electron microscope often requires element distribution images. Until recently, element mapping was strongly limited by the count rate that could be processed with conventional EDS systems using Si(Li) or Ge crystal diode detectors. The enhancement of secondary and backscattered SEM images can now be achieved through the addition of rapid color imaging. Using a new methodology for color enhancement, features as small as 1 micrometer can be colored and easily distinguished. The addition of color to SEM images enables easy visualization of element distribu

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Conforto, Egle, Nicolas Joguet, Pierre Buisson, Jean-Eudes Vendeville, Carine Chaigneau, and Thierry Maugard. "An optimized methodology to analyze biopolymer capsules by environmental scanning electron microscopy." Materials Science and Engineering: C 47 (February 2015): 357–66. http://dx.doi.org/10.1016/j.msec.2014.11.054.

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Login, G. R., W. B. Stavinoha, and A. M. Dvorak. "Ultrafast microwave energy fixation for electron microscopy." Journal of Histochemistry & Cytochemistry 34, no. 3 (March 1986): 381–87. http://dx.doi.org/10.1177/34.3.3950387.

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We demonstrate that microwave (MW) energy can be used in conjunction with chemical cross-linking agents to fix tissue blocks rapidly for electron microscopy in as brief a time as 26 msec. The optimal ultrafast MW fixation methodology involved immersing tissue blocks up to 2 mm3 in dilute aldehyde fixative and immediately irradiating the specimens in a 7.3 kW MW oven for 26-90 msec, reaching a fixation temperature range of 32-42 degrees C. Ultrastructural preservation of samples irradiated by MW energy was comparable to that of the control samples immersed in aldehyde fixative for 2 hr at 25 de

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16

Drab, Marek, Janusz Krajniak, and Krzysztof P. Grzelakowski. "The New Methodology and Chemical Contrast Observation by Use of the Energy-Selective Back-Scattered Electron Detector." Microscopy and Microanalysis 22, no. 6 (December 2016): 1369–73. http://dx.doi.org/10.1017/s1431927616012514.

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AbstractWe report on a robust method for chemical element-sensitive imaging by scanning electron microscopy (SEM). The commercial Auriga FE-SEM microscope (Carl Zeiss, Oberkochen, Germany), equipped with an energy-selective grid detector (EsB) as a part of the experimental setup, was applied for generation of chemical contrast at low accelerating voltages, which is gentle for sensitive samples. The EsB-grid detector, conceptually adapted by us as an energy retarding field analyzer (RFA), was used to detect the two-dimensional (2D) energy spectrum for the first time. The electron energy spectru

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Baumann, Frieder, Pradip Sairam Pichumani, and Christopher Torcedo. "Innovative Grounding Methodology for Epoxy Impregnated Semiconductor Cross Sections for Electron Microscopy Inspection." Microscopy and Microanalysis 26, S2 (July 30, 2020): 370–72. http://dx.doi.org/10.1017/s1431927620014415.

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Tufvesson, Bertil, and Torsten Johnsson. "IMMUNOELECTROOSMOPHORESIS FOR DETECTION OF REO-LIKE VIRUS: METHODOLOGY AND COMPARISON WITH ELECTRON MICROSCOPY." Acta Pathologica Microbiologica Scandinavica Section B Microbiology 84B, no. 4 (August 15, 2009): 225–28. http://dx.doi.org/10.1111/j.1699-0463.1976.tb01929.x.

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Yurtsever, Aycan, Renske M. van der Veen, and Ahmed H. Zewail. "Subparticle Ultrafast Spectrum Imaging in 4D Electron Microscopy." Science 335, no. 6064 (January 5, 2012): 59–64. http://dx.doi.org/10.1126/science.1213504.

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Single-particle imaging of structures has become a powerful methodology in nanoscience and molecular and cell biology. We report the development of subparticle imaging with space, time, and energy resolutions of nanometers, femtoseconds, and millielectron volts, respectively. By using scanning electron probes across optically excited nanoparticles and interfaces, we simultaneously constructed energy-time and space-time maps. Spectrum images were then obtained for the nanoscale dielectric fields, with the energy resolution set by the photon rather than the electron, as demonstrated here with tw

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Zehbe, Rolf, Astrid Haibel, Heinrich Riesemeier, Ulrich Gross, C. James Kirkpatrick, Helmut Schubert, and Christoph Brochhausen. "Going beyond histology. Synchrotron micro-computed tomography as a methodology for biological tissue characterization: from tissue morphology to individual cells." Journal of The Royal Society Interface 7, no. 42 (March 25, 2009): 49–59. http://dx.doi.org/10.1098/rsif.2008.0539.

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Current light microscopic methods such as serial sectioning, confocal microscopy or multiphoton microscopy are severely limited in their ability to analyse rather opaque biological structures in three dimensions, while electron optical methods offer either a good three-dimensional topographic visualization (scanning electron microscopy) or high-resolution imaging of very thin samples (transmission electron microscopy). However, sample preparation commonly results in a significant alteration and the destruction of the three-dimensional integrity of the specimen. Depending on the selected photon

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Takizawa, Toshihiro, and John M. Robinson. "FluoroNanogold Is a Bifunctional Immunoprobe for Correlative Fluorescence and Electron Microscopy." Journal of Histochemistry & Cytochemistry 48, no. 4 (April 2000): 481–85. http://dx.doi.org/10.1177/002215540004800405.

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We applied a fluorescent ultrasmall immunogold probe, FluoroNanogold (FNG), to immunocytochemistry on ultrathin cryosections. FNG has the properties of both a fluorescent dye-conjugated antibody for fluorescence microscopy and a gold particle-conjugated antibody for electron microscopy. Therefore, this bifunctional immunoprobe permits correlative microscopic observation of the same cell profiles labeled in a single labeling procedure by these two imaging methods. We demonstrate the utility of FNG as a secondary antibody for immunocytochemical labeling of myeloperoxidase (a marker protein for a

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Castroviejo, R., E. Berrezueta, and R. Lastra. "Microscopic digital image analysis of gold ores: a critical test of methodology, comparing reflected light and electron microscopy." Mining, Metallurgy & Exploration 19, no. 2 (May 2002): 102–9. http://dx.doi.org/10.1007/bf03403263.

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Dejgaard, Selma Yilmaz, Ayesha Murshid, Kristina M. Dee, and John F. Presley. "Confocal Microscopy-based Linescan Methodologies for Intra-Golgi Localization of Proteins." Journal of Histochemistry & Cytochemistry 55, no. 7 (March 19, 2007): 709–19. http://dx.doi.org/10.1369/jhc.6a7090.2007.

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Localization of resident Golgi proteins to earlier ( cis) or later ( trans) Golgi compartments has traditionally required quantitative immunocytochemistry and electron microscopy, which are inaccessible to many researchers. For this reason, light microscopy has often been used, initially for localization of Golgi glycotransferases and, more recently, for other Golgi proteins (e.g., Arf1, GBF1, Rab6). Quantitation of light microscopic intra-Golgi localization can be problematic. We describe here a novel quantitative light microscopic methodology using linescans crossing the Golgi ribbon. Our me

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Sakamoto, Hirotaka, and Mitsuhiro Kawata. "Ultrahigh Voltage Electron Microscopy Links Neuroanatomy and Neuroscience/Neuroendocrinology." Anatomy Research International 2012 (December 8, 2012): 1–5. http://dx.doi.org/10.1155/2012/948704.

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The three-dimensional (3D) analysis of anatomical ultrastructures is extremely important in most fields of biological research. Although it is very difficult to perform 3D image analysis on exact serial sets of ultrathin sections, 3D reconstruction from serial ultrathin sections can generally be used to obtain 3D information. However, this technique can only be applied to small areas of a specimen because of technical and physical difficulties. We used ultrahigh voltage electron microscopy (UHVEM) to overcome these difficulties and to study the chemical neuroanatomy of 3D ultrastructures. This

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Goheen, Michael P., Marilyn S. Bartlett, and James W. Smith. "Immunogold labeling of Pneumocystis carinii for Electron microscopy." Proceedings, annual meeting, Electron Microscopy Society of America 49 (August 1991): 270–71. http://dx.doi.org/10.1017/s0424820100085654.

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Studies of the biology of Pneumocystis carinii (PC) are of increasing importance because this extracellular pathogen is a frequent source of severe pneumonia in patients with acquired immunodeficiency syndrome (AIDS) and is a leading cause of mortality in these patients. Immunoelectron microscopic localization of antigenic sites on the surface of PC would improve the understanding of these sites and their role in pathenogenisis of the disease and response to chemotherapy. The purpose of this study was to develop a methodology for visualizing immunoreactive sites on PC with transmission electro

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Hayakawa, Eri H., and Hiroyuki Matsuoka. "Detailed methodology for high resolution scanning electron microscopy (SEM) of murine malaria parasitized-erythrocytes." Parasitology International 65, no. 5 (October 2016): 539–44. http://dx.doi.org/10.1016/j.parint.2016.03.006.

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Stoeck, Thorsten, William H. Fowle, and Slava S. Epstein. "Methodology of Protistan Discovery: from rRNA Detection to Quality Scanning Electron Microscope Images." Applied and Environmental Microbiology 69, no. 11 (November 2003): 6856–63. http://dx.doi.org/10.1128/aem.69.11.6856-6863.2003.

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ABSTRACT Each year, thousands of new protistan 18S rRNA sequences are detected in environmental samples. Many of these sequences are molecular signatures of new protistan species, classes, and/or kingdoms that have never been seen before. The main goal of this study was to enable visualization of these novel organisms and to conduct quality ultrastructural examination. We achieved this goal by modifying standard procedures for cell fixation, fluorescence in situ hybridization, and scanning electron microscopy (SEM) and by making these methodologies work in concert. As a result, the same indivi

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Bykov, Yury S., Nir Cohen, Natalia Gabrielli, Hetty Manenschijn, Sonja Welsch, Petr Chlanda, Wanda Kukulski, Kiran R. Patil, Maya Schuldiner, and John A. G. Briggs. "High-throughput ultrastructure screening using electron microscopy and fluorescent barcoding." Journal of Cell Biology 218, no. 8 (July 9, 2019): 2797–811. http://dx.doi.org/10.1083/jcb.201812081.

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Genetic screens using high-throughput fluorescent microscopes have generated large datasets, contributing many cell biological insights. Such approaches cannot tackle questions requiring knowledge of ultrastructure below the resolution limit of fluorescent microscopy. Electron microscopy (EM) reveals detailed cellular ultrastructure but requires time-consuming sample preparation, limiting throughput. Here we describe a robust method for screening by high-throughput EM. Our approach uses combinations of fluorophores as barcodes to uniquely mark each cell type in mixed populations and correlativ

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Kang, J., M. Jain, D. S. Wilkinson, and J. D. Embury. "Microscopic Strain Mapping Using Scanning Electron Microscopy Topography Image Correlation at Large Strain." Journal of Strain Analysis for Engineering Design 40, no. 6 (August 1, 2005): 559–70. http://dx.doi.org/10.1243/030932405x16151.

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Measuring the distribution of local strain at the microscopic level is a challenging problem, especially for materials subjected to large overall strain. In the present study, a novel microscopic strain mapping technique has been developed based on the analysis of surface topography using digital image correlation (DIC) software. The input is a series of scanning electron microscopy (SEM) images. The method uses topographic features (such as surface slip traces) found in these images as the input. A commercially available optical strain measurement system (ARAMIS®, which is a trade name of the

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Arnold, Christopher, Christoph Pobel, Fuad Osmanlic, and Carolin Körner. "Layerwise monitoring of electron beam melting via backscatter electron detection." Rapid Prototyping Journal 24, no. 8 (November 12, 2018): 1401–6. http://dx.doi.org/10.1108/rpj-02-2018-0034.

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Purpose The purpose of this study is the introduction and validation of a new technique for process monitoring during electron beam melting (EBM). Design/methodology/approach In this study, a backscatter electron detector inside the building chamber is used for image acquisition during EBM process. By systematic variation of process parameters, the ability of displaying different topographies, especially pores, is investigated. The results are evaluated in terms of porosity and compared with optical microscopy and X-ray computed tomography. Findings The method is capable of detecting major fla

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Taheri, Shima, Martin Ams, Heriberto Bustamante, Louisa Vorreiter, Michael Withford, and Simon Martin Clark. "A practical methodology to assess corrosion in concrete sewer pipes." MATEC Web of Conferences 199 (2018): 06010. http://dx.doi.org/10.1051/matecconf/201819906010.

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The combination of Neutron Tomography (NT), Scanning Electron Microscopy (SEM, with and without elemental analysis), pH profiling and Microindentation Mapping techniques was used to develop a methodology for the assessment of concrete sewer pipe deterioration. We demonstrate a viable method for health monitoring of concrete sewer pipes and the evaluation of competing mitigation strategies.

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Duan, Heng-Zhi, Hong-Yan Zeng, Hua-Miao Xiao, Chao-Rong Chen, Gao-Fei Xiao, and Quan Zhao. "Optimization of ammonia nitrogen removal by SO42− intercalated hydrotalcite using response surface methodology." RSC Advances 6, no. 54 (2016): 48329–35. http://dx.doi.org/10.1039/c6ra08321h.

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SO<sub>4</sub><sup>2−</sup> intercalated Mg–Al hydrotalcite (S-LDH) was prepared under microwave irradiation and characterized by powder X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM).

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Rzepiejewska-Malyska, K. A., W. M. Mook, M. Parlinska-Wojtan, J. Hejduk, and J. Michler. "In situ scanning electron microscopy indentation studies on multilayer nitride films: Methodology and deformation mechanisms." Journal of Materials Research 24, no. 3 (March 2009): 1208–21. http://dx.doi.org/10.1557/jmr.2009.0139.

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Systematic studies of the deformation mechanisms of multilayer transition metal nitride coatings TiN/CrN, TiN/NbN, and NbN/CrN, and corresponding reference coatings of TiN, NbN, and CrN deposited by a direct current (dc) magnetron sputtering process onto silicon 〈100〉 have been performed. Mechanical characterization was conducted using a combination of microindentation and nanoindentation in the load range 30 to 150 mN and 0.5 to 3.5 mN, respectively. For both load ranges, scanning electron microscopy (SEM) in situ indentation was used to observe the indentation process including any pileup, s

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Stanley, Shena M., Amartya Chakrabarti, Joshua J. DeMuth, Vanessa E. Tempel, and Narayan S. Hosmane. "Catalyst-Free Bottom-Up Synthesis of Few-Layer Hexagonal Boron Nitride Nanosheets." Journal of Nanomaterials 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/304295.

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A novel catalyst-free methodology has been developed to prepare few-layer hexagonal boron nitride nanosheets using a bottom-up process. Scanning electron microscopy and transmission electron microscopy (both high and low resolution) exhibit evidence of less than ten layers of nanosheets with uniform dimension. X-ray diffraction pattern and other additional characterization techniques prove crystallinity and purity of the product.

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da Silva, Marcelo A., José Claudio G. Teixeira, Ari Sauer Guimarães, Ivani S. Bott, and Hector R. M. Costa. "A Methodology for Evaluation of Life Fraction Using Cr-Mo Steel under Creep Conditions." Materials Science Forum 758 (June 2013): 57–63. http://dx.doi.org/10.4028/www.scientific.net/msf.758.57.

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The quantification methodology used to predict the residual life of Cr-Mo steel was microstructure/life fraction correlation under creep conditions. Microstructural evolution has been correlated with accelerated creep testing progress during testing at constant load and a temperature of 600°C, as well as with scanning electron microscopy (SEM) and transmission electron microscopy (TEM) analysis. Experimental results from TEM revealed six distinct stages, which were directly related to the life fraction values obtained. However, in the case of MEV, five stages were found. This difference is lik

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Wang, Haifeng, Jason Fang, Jason Arjavac, and Rudy Kellner. "Scanning Transmission Electron Microscopy for Critical Dimension Monitoring in Wafer Manufacturing." Microscopy Today 16, no. 1 (January 2008): 24–27. http://dx.doi.org/10.1017/s1551929500054298.

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Automated scanning transmission electron microscopy (STEM) metrology provides critical dimension (CD) measurements an order of magnitude more precise than comparable scanning electron microscopy (SEM) measurements. New developments in automation now also provide throughput and response time sufficient to support high volume microelectronic manufacturing processes. The newly developed methodology includes automated, focused ion beam (FIB) based sample preparation; innovative, ex-situ sample extraction; and automated metrology. Although originally developed to control the production of thin film

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Baker, T. S., and D. A. Winkelmann. "Methodology for determining the three-dimensional crystal structure of myosin Sl from electron microscopy of orthogonal thin sections." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 26–29. http://dx.doi.org/10.1017/s0424820100141895.

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Detailed knowledge of the structure of myosin is essential for understanding the mechanism of muscle contraction. The discovery of conditions for crystallizing the head of myosin (Sl = subfragment 1) has led to systematic studies of the SI structure by x-ray crystallography and electron microscopy. We describe the method used to determine the structure of the myosin Sl molecule by electron microscopy (Fig. 3). This method involved independently reconstructing the three-dimensional density of several thin sections obtained from oriented crystals and then combining these reconstructions to produ

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Cone, Kim A., Richard F. Wendlandt, Katharina Pfaff, and Omero F. Orlandini. "Texture constraints on crystal size distribution methodology: An application to the Laki fissure eruption." American Mineralogist 105, no. 5 (May 1, 2020): 585–98. http://dx.doi.org/10.2138/am-2020-7007.

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Abstract Modeling crystal size distributions often requires the extraction of 2D discrete crystal lengths to calculate 3D volumetric equivalences. These apparent lengths are obtained from digital images that exploit different physical and chemical characteristics of samples, and the choice of image type can affect the interpretation of crystal length measurements, thus affecting crystal size distribution modeling. To examine method- and texture-based effects on extracting crystal size distributions, we obtained plagioclase length measurements from two texturally opposing basaltic lava samples

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Yi, Hong, Rahmat Peijper, and Jan LM Leunissen. "Methodology Advancements in Electron Microscopy Immunolabeling of Hydrated Brain Tissue Using Subnanometer Colloidal Gold Conjugates." Microscopy and Microanalysis 8, S02 (August 2002): 126–27. http://dx.doi.org/10.1017/s1431927602101644.

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Ebrahimi, Shamsi, Coswald Stephen Sipaut@ Mohd Nasri, and Sazmal Effendi Bin Arshad. "Hydrothermal synthesis of hydroxyapatite powders using Response Surface Methodology (RSM)." PLOS ONE 16, no. 5 (May 20, 2021): e0251009. http://dx.doi.org/10.1371/journal.pone.0251009.

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Hydroxyapatite (HAp)—[Ca10 (PO4)6(OH) 2] has a similar chemical composition to bone material, making it the main mineral supplement in bone-making. Due to its high biocompatibility, hydroxyapatite is widely used in the repair of bone deficiencies and in the production of dental or orthopedic implants. In this research, hydroxyapatite nanopowder was synthesized using a hydrothermal technique. Fourier Transform Infrared Spectroscopy (FTIR) and transmission electron microscopy (TEM) were used to investigate the chemical structure and morphology of the synthesized hydroxyapatite powder. X-ray diff

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Honary, Soheila, Hamed Barabadi, Pouneh Ebrahimi, Farzaneh Naghibi, and Ahad Alizadeh. "Development and Optimization of Biometal Nanoparticles by Using Mathematical Methodology: A Microbial Approach." Journal of Nano Research 30 (March 2015): 106–15. http://dx.doi.org/10.4028/www.scientific.net/jnanor.30.106.

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This study aimed to biosynthesize and optimize the process of iron oxide nanoparticles producing by Penicilliumwaksmanii isolated from soil by employing mathematical methodology. The synthesized nanoparticles were formed with fairly well-defined dimensions with good monodispersity determined by SEM (Scanning Electron Microscopy), AFM (Atomic Force Microscopy), DLS (Dynamic Light Scattering), UV-Visible spectroscopy, zeta potential, polydispersity index (PDI) and correlogram of nanoparticles. The effects of different factors such as pH, temperature and concentration of FeCl3 on the particle siz

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Steven, Alasdair C. "Breakthroughs and breakdowns: the portrayal of biological macromolecules in computer-processed images of negatively stained specimens." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 62–65. http://dx.doi.org/10.1017/s0424820100142025.

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Negative staining - the contrast enhancement technique of applying solutions of heavy metal salts to macromolecular particles whose structures are ultimately inferred from the distribution of electron-opaque material that envelopes them - was introduced almost thirty years ago. Since then, the technique has undergone many modifications and refinements, but remains perhaps the most widely used method of preparing isolated biological macromolecules for electron microscopic observation. The advent of image processing, with its potentialities for noise reduction and three-dimensional reconstructio

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Faas, Frank G. A., M. Cristina Avramut, Bernard M. van den Berg, A. Mieke Mommaas, Abraham J. Koster, and Raimond B. G. Ravelli. "Virtual nanoscopy: Generation of ultra-large high resolution electron microscopy maps." Journal of Cell Biology 198, no. 3 (August 6, 2012): 457–69. http://dx.doi.org/10.1083/jcb.201201140.

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A key obstacle in uncovering the orchestration between molecular and cellular events is the vastly different length scales on which they occur. We describe here a methodology for ultrastructurally mapping regions of cells and tissue as large as 1 mm2 at nanometer resolution. Our approach employs standard transmission electron microscopy, rapid automated data collection, and stitching to create large virtual slides. It greatly facilitates correlative light-electron microscopy studies to relate structure and function and provides a genuine representation of ultrastructural events. The method is

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Wen, Haiming, Yaojun Lin, David N. Seidman, Julie M. Schoenung, Isabella J. van Rooyen, and Enrique J. Lavernia. "An Efficient and Cost-Effective Method for Preparing Transmission Electron Microscopy Samples from Powders." Microscopy and Microanalysis 21, no. 5 (September 9, 2015): 1184–94. http://dx.doi.org/10.1017/s1431927615014695.

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AbstractThe preparation of transmission electron microcopy (TEM) samples from powders with particle sizes larger than ~100 nm poses a challenge. The existing methods are complicated and expensive, or have a low probability of success. Herein, we report a modified methodology for preparation of TEM samples from powders, which is efficient, cost-effective, and easy to perform. This method involves mixing powders with an epoxy on a piece of weighing paper, curing the powder–epoxy mixture to form a bulk material, grinding the bulk to obtain a thin foil, punching TEM discs from the foil, dimpling t

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Paterson, Gary W., Gavin M. Macauley, Stephen McVitie, and Yoshihiko Togawa. "Parallel Mode Differential Phase Contrast in Transmission Electron Microscopy, I: Theory and Analysis." Microscopy and Microanalysis 27, no. 5 (September 20, 2021): 1113–22. http://dx.doi.org/10.1017/s1431927621012551.

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In Part I of this diptych, we outline the parallel mode of differential phase contrast (TEM-DPC), which uses real-space distortion of Fresnel images arising from electrostatic or magnetostatic fields to quantify the phase gradient of samples with some degree of structural contrast. We present an analysis methodology and the associated software tools for the TEM-DPC method and, using them together with numerical simulations, compare the technique to the widely used method of phase recovery based on the transport-of-intensity equation (TIE), thereby highlighting the relative advantages and limit

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Huh, PilHo, and Seong-Cheol Kim. "Nanostructured ZnO Arrays with Self-ZnO Layer Created Using Simple Electrostatic Layer-by-Layer Assembly." Journal of Nanomaterials 2012 (2012): 1–6. http://dx.doi.org/10.1155/2012/131672.

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Formation of unique ZnO nanoarrays utilizing photodynamic polymer, surface-relief grating structures, and unique electrostatic layer-by-layer assembly as a simple and economical methodology was demonstrated. Atomic force microscope (AFM), scanning electron microscopy (SEM), and energy-dispersive X-ray (EDAX) analysis were employed to characterize elemental composition and morphology of the resulting ZnO nanostructures with self-ZnO layer. Optical behavior of the final product was studied by UV-vis-NIR absorption and photoluminescence (PL) spectra.

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Karimi Sabet, Javad, Cyrus Ghotbi, and Farid Dorkoosh. "Application of Response Surface Methodology for Optimization of Paracetamol Particles Formation by RESS Method." Journal of Nanomaterials 2012 (2012): 1–15. http://dx.doi.org/10.1155/2012/340379.

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Ultrafine particles of paracetamol were produced by Rapid Expansion of Supercritical Solution (RESS). The experiments were conducted to investigate the effects of extraction temperature (313–353 K), extraction pressure (10–18 MPa), preexpansion temperature (363–403 K), and postexpansion temperature (273–323 K) on particles size and morphology of paracetamol particles. The characterization of the particles was determined by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Liquid Chromatography/Mass Spectrometry (LC-MS) analysis. The average particle size of the or

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Batuk, Dmitry, Maria Batuk, Artem M. Abakumov, and Joke Hadermann. "Synergy between transmission electron microscopy and powder diffraction: application to modulated structures." Acta Crystallographica Section B Structural Science, Crystal Engineering and Materials 71, no. 2 (March 31, 2015): 127–43. http://dx.doi.org/10.1107/s2052520615005466.

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The crystal structure solution of modulated compounds is often very challenging, even using the well established methodology of single-crystal X-ray crystallography. This task becomes even more difficult for materials that cannot be prepared in a single-crystal form, so that only polycrystalline powders are available. This paper illustrates that the combined application of transmission electron microscopy (TEM) and powder diffraction is a possible solution to the problem. Using examples of anion-deficient perovskites modulated by periodic crystallographic shear planes, it is demonstrated what

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GAJDARDZISKA-JOSIFOVSKA, M., M. H. MALAY, and DAVID J. SMITH. "REFLECTION ELECTRON MICROSCOPY METHODOLOGY FOR QUANTIFICATION OF CLUSTER GROWTH: INDIUM CLUSTERS ON THE InP(110) SURFACE." Surface Review and Letters 04, no. 04 (August 1997): 655–69. http://dx.doi.org/10.1142/s0218625x97000651.

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Dynamical reflection electron microscopy (REM) can provide a wealth of time-resolved data pertinent to the initial and intermediate stages of cluster growth. REM allows one to follow and quantify the size and shape evolution of individual clusters. Average cluster size and cluster density data can be obtained as a function of time due to the large field of view produced by REM image foreshortening. We describe here a methodology for extracting these data from dynamical REM experiments, based on a geometrical model for the interpretation of REM images from two- and three-dimensional clusters. T

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Lin, Wan-Hsien, Uwe Jahn, Hanno Küpers, Esperanza Luna, Ryan B. Lewis, Lutz Geelhaar, and Oliver Brandt. "Efficient methodology to correlate structural with optical properties of GaAs nanowires based on scanning electron microscopy." Nanotechnology 28, no. 41 (September 13, 2017): 415703. http://dx.doi.org/10.1088/1361-6528/aa8394.

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