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Rozprawy doktorskie na temat „Enzymes Synthesis”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 6 września 2023

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1

Ekici, Özlem Doğan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Available online, Georgia Institute of Technology, 2004:, 2003. http://etd.gatech.edu/theses/available/etd-04062004-164633/unrestricted/ekici%5Fozlem%5Fd%5F200312%5Fphd.pdf.

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2

Ekici, Ozlem Dogan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Diss., Georgia Institute of Technology, 2003. http://hdl.handle.net/1853/5333.

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3

Naylor, Neil J. "Biotransformations involving hydrolytic enzymes." Thesis, University of Warwick, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263607.

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4

Silveira, Alvito J. "Synthesis of 6-guanidinobenzoxazinones as potential inhibitors of trypsin-like enzymes." Thesis, Georgia Institute of Technology, 1989. http://hdl.handle.net/1853/26914.

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5

Snider, Catherine E. "Synthesis and biochemical evaluation of irreversible inhibitors of aromatase /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487266362338344.

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6

Carnell, Andrew John. "Cycloalkanone monooxygenase enzymes in organic synthesis." Thesis, University of Exeter, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293971.

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7

Blum, Janna Karen. "Broadening the enyzme-catalyzed synthesis of semi-synthetic antibiotics." Diss., Georgia Institute of Technology, 2011. http://hdl.handle.net/1853/39528.

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An alpha-amino ester hydrolase (AEH) applicable to synthesis of semi-synthetic antibiotics was cloned from the genomic DNA of Xanthomonas campestris pv. campestris sp. strain ATCC 33913. AEHs catalyze the synthesis and hydrolysis of alpha-amino beta-lactam antibiotics. The enzyme was characterized for thermodynamic and kinetic parameters. The enzyme shows optimal ampicillin hydrolytic activity at 25C and pH 6.8. The AEH enzymes have been shown to have excellent synthetic capability. Additionally, we demonstrated the first fully aqueous enzymatic one-pot synthesis of ampicillin direct from the

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8

Admans, Gary David. "Asymmetric transformations catalysed by lipase enzymes." Thesis, University of Exeter, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240288.

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9

Chambers, Martina Natasha. "Synthesis of cellulosic glycolipids using engineered enzymes." Thesis, University of British Columbia, 2014. http://hdl.handle.net/2429/46032.

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Cellulose, a linear polymer of D-glucose units connected by β-1,4 glycosidic bonds, adopts a highly-ordered crystalline structure in solution. In cellulose I, the dominant form of cellulose in nature, the polymeric chains are aligned in the same direction. Previous attempts to synthesize cellulose I in vitro have resulted in the synthesis of cellulose II, which has the thermodynamically favored anti-parallel orientation of chains. The synthesis of soluble fragments or defined surfaces of cellulose I would enable more detailed study of carbohydrate binding domains and other proteins that intera

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10

Ahmed, Naveed. "The synthesis of inhibitors of proteolytic enzymes." Thesis, University of Huddersfield, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416765.

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11

Davis, Benjamin G. "Synthesis of inhibitors of sugar processing enzymes." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.711593.

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12

Dotse, Anthony Kwabla. "Design, synthesis and evaluation of novel inhibitors of cysteine proteases, metalloproteases and the proteasome, a unique high molecular weight proteolytic enzyme." Diss., Georgia Institute of Technology, 1997. http://hdl.handle.net/1853/29979.

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13

Parekh, Parag A. "Bioprocess development for asymmetric reductions by Saccharomyces cerevisiae enzymes." [Gainesville, Fla.] : University of Florida, 2006. http://purl.fcla.edu/fcla/etd/UFE0014371.

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14

Qiu, Jian. "Synthesis of inhibitors of terpene and shikimate enzymes." Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627500.

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15

Guilly, Selena. "Synthesis of novel acceptor substrates for glycosyltransferase enzymes." Thesis, University of Edinburgh, 2004. http://hdl.handle.net/1842/16997.

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Our research has been based on the biosynthetic pathway to <i>N</i>-linked glycoproteins, species which are comprised of an oligosaccharide moiety attached to the side-chain nitrogen atom of an asparagines residue in a peptide. In order to effectively study the steps involved in the biosynthesis of lipid-linked oligosaccharides and their subsequent transfer to a nascent protein in cotranslational modification processes, it is essential to have a source of pure dolichols in quantities sufficient to allow the synthesis of both biosynthetic intermediates and synthetic analogues in the pathways re

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16

Mazda, Xerxes Cyrus. "Synthesis of substrate analogues for terpene cyclase enzymes." Thesis, University of Cambridge, 1992. https://www.repository.cam.ac.uk/handle/1810/272667.

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17

Holt, Karen Elizabeth. "Asymmetric synthesis of aza-sugars using aldolase enzymes." Thesis, University of Cambridge, 1993. https://www.repository.cam.ac.uk/handle/1810/272658.

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18

Vázquez-Figueroa, Eduardo. "Development of a novel dehydrogenase and a stable cofactor regeneration system." Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/31685.

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Thesis (Ph.D)--Chemical Engineering, Georgia Institute of Technology, 2009.<br>Committee Chair: Bommarius, Andreas S.; Committee Member: Doyle, Donald F.; Committee Member: Koros, William J.; Committee Member: Moore, Jeffre C.; Committee Member: Prausnitz, Mark R. Part of the SMARTech Electronic Thesis and Dissertation Collection.

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19

Heng, Sabrina. "The Design, Synthesis and Characterization of Enzyme Inhibitors." Thesis, Boston College, 2009. http://hdl.handle.net/2345/755.

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Thesis advisor: Evan R. Kantrowitz<br>Thesis advisor: Mary F. Roberts<br>In this work, a series of inhibitors of two enzymes, aspartate transcarbamoylase (ATCase) and fructose 1,6-bisphosphatase (FBPase) were designed, synthesized and characterized. ATCase catalyzes the committed step in pyrimidine nucleotide biosynthesis, the reaction between carbamoyl phosphate (CP) and L-aspartate to form N-carbamoyl-L-aspartate (CA) and inorganic phosphate. This step is exceptionally important because once CA is formed, it is committed to the biosynthesis of pyrimidines, a necessary component for nucleic a

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20

Hinchliffe, Paul Stuart. "The synthesis of #beta#-sultam inhibitors of proteolytic enzymes." Thesis, University of Huddersfield, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.368328.

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21

Bingham, Matilda Jane. "Insights into the design and synthesis of artificial enzymes." Thesis, University College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395525.

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22

Lemoult, Stephanie Claudette. "Biocatalysis in organic synthesis using microorganisms and immobilised enzymes." Thesis, University of Exeter, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.260622.

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23

Newgas, Sophie Alice. "Biocatalysis using plant and metagenomic enzymes for organic synthesis." Thesis, University College London (University of London), 2018. http://discovery.ucl.ac.uk/10052003/.

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Biocatalysts provide an excellent alternative to traditional organic chemistry strategies, with advantages such as mild reaction conditions and high enantio- and stereoselectivities. The use of metagenomics has enabled new enzymes to be sourced with high sequence diversity. At UCL a metagenomics strategy has been developed for enzyme discovery, in which the library generated is annotated and searched for desired enzyme sequences. In this PhD, a metagenomic approach was used to retrieve 37 short chain reductase/dehydrogenases (SDRs) from an oral environment metagenome. Eight enzymes displayed a

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24

Scaffidi, Adrian. "Synthetic endeavours in carbohydrates." University of Western Australia. School of Biomedical, Biomolecular and Chemical Sciences, 2007. http://theses.library.uwa.edu.au/adt-WU2007.0114.

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The overwhelming occurrence and structural diversity of carbohydrates in Nature indicate their importance in a range of fundamental life processes. Indeed, it is this diversity that has lead to the two equally diverse groups of carbohydrate-processing enzymes, namely the glycoside hydrolases and glycosyl transferases. Thus, understanding the role of both carbohydrates and their processing enzymes in biological systems has attracted significant attention. This thesis, firstly, describes endeavours towards the synthesis of an inositol ?- amino acid, along with a series of sugar α-substituted car

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25

Chada, Sravanthi. "A new synthetic approach for preparation of efavirenz." Thesis, Nelson Mandela Metropolitan University, 2017. http://hdl.handle.net/10948/15512.

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Efavirenz, a drug that is still inaccessible to millions of people worldwide, is potent non nucleoside reverse transcriptase inhibitor (NNRTI), is one of the preferred agents used in combination therapy for first-line treatment of the human immunodeficiency virus (HIV). NNRTIs attach to and block an HIV enzyme called reverse transcriptase, by blocking reverse transcriptase; NNRTIs prevent HIV from multiplying and can reduce the amount of HIV in the body. Efavirenz can't cure HIV/AIDS, but taken in combination with other HIV medicines (called an HIV regimen) every day helps people with HIV live

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26

Oldfield, C. "The behaviour of enzymes in water-in-oil microemulsion systems." Thesis, University of Kent, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.380705.

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27

Vousden, William Alexander. "Mutational analysis of the ACV synthetase gene of Aspergillus nidulans." Thesis, University of Sheffield, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366097.

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28

Sutherland, Andrew. "The enantioselective synthesis of amino acids for the investigation of protein structure." Thesis, University of Bristol, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388029.

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29

Clough, Sarah. "Routes to the synthesis of oxygen containing heterocycles." Thesis, University of Bristol, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301998.

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30

Feng, Jun. "A kinetic investigation of recombinant xenopus laevis amidating enzymes." Diss., Georgia Institute of Technology, 1999. http://hdl.handle.net/1853/30084.

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31

Sampson, Peter B. "Synthesis of potential inhibitors targeting enzymes involved in methionine biochemistry." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape3/PQDD_0018/NQ53511.pdf.

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32

Johnson, Dean Vincent. "The synthesis of optically active chiral building blocks using enzymes." Thesis, University of Exeter, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317396.

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33

Watt, Alan. "Synthesis of novel benzotriazole substrates suitable for screening hydrolytic enzymes." Thesis, University of Edinburgh, 2002. http://hdl.handle.net/1842/11529.

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34

Lin, Jay. "Enzymes in thymidylate synthesis in Ureaplasma parvum as medical targets /." Uppsala : Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, 2009. http://epsilon.slu.se/11487069.pdf.

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35

Ndi, Cornelius Ndi. "Synthesis of Chemical Models of Hydrolase Enzymes for Intramolecular Catalysis." Digital Commons @ East Tennessee State University, 2011. https://dc.etsu.edu/etd/1356.

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Most nuclease enzymes can hydrolyze phosphoester bonds (in DNA and RNA) by using metal ions cofactors that coordinate and activate water molecules in the enzymes' active sites. However, there are some hydrolase enzymes (including nucleases) that can function without the aid of metal ions. 2,6-Di(1H-imidazol-2-yl)phenol, a model compound for hydrolase enzyme, was synthesized by the reaction between ethylenediamine and dimethyl-3-carboxysalicylate, initially resulting in the formation of diimidazoline. The diimidazoline was subsequently aromatized to the diimidazole by dehydrogenation over palla

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36

Chen, Allen Kuan-Liang Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Enhanced biocatalyst production for (R)-phenylacetylcarbinol synthesis." Awarded by:University of New South Wales. School of Biotechnology and Biomolecular Sciences, 2006. http://handle.unsw.edu.au/1959.4/32825.

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The enzymatic production of R-phenylacetylcarbinol (R-PAC), with either whole cells or partially purified pyruvate decarboxylase (PDC) as the biocatalyst, requires high PDC activity and an inexpensive source of pyruvate for an economical feasible biotransformation process. Microbial pyruvate produced by a vitamin auxotrophic strain of Candida glabrata was selected as a potential substrate for biotransformation. With an optimal thiamine concentration of 60 ??g/l, a pyruvic acid concentration of 43 g/l and yield of 0.42 g/g glucose consumed were obtained. Using microbially-produced unpurified py

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37

Rickaby, Kirstie. "Investigation of the chemo-enzymatic synthesis of cyclic peptides." Thesis, University of Aberdeen, 2018. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=238613.

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Cyclic peptides constitute an attractive class of compounds for drug development, however the numerous problems associated with their synthesis have limited their applicability. The cyclisation step itself is particularly problematic, with solution phase cyclisations being required to be conducted under very high dilution to promote cyclisation over unwanted side reactions such as oligomerisation. In addition, epimerisation, leading to the loss of chiral integrity at the terminal residues is a major concern. Attention is now turning to biochemical cyclisation strategies, such as SICLOPPS and s

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38

Simkovsky, Nadja Melitta. "Synthesis of some potential IKK inhibitors based around a pyrimidine scaffold." Thesis, University of Liverpool, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.367619.

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39

Maddrell, Samuel James. "The application of organonitrile compounds to asymmetric synthesis." Thesis, University of Exeter, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294473.

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40

Aitken, D. J. "Approaches to selective synthesis using modified enzyme systems." Thesis, University of Strathclyde, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381516.

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41

Khan, Ahmed M. "Cyclic enzymatic solid phase synthesis of DNA oligonucleotides on an epoxide-activated resin [electronic resource] /." unrestricted, 2008. http://etd.gsu.edu/theses/available/etd-05082008-163131/.

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Thesis (M.S.)--Georgia State University, 2008.<br>Title from file title page. Markus W. Germann, committee chair; W. David Wilson, Kathryn B. Grant, committee members. Electronic text (51 p. : ill. (some col.)) : digital, PDF file. Description based on contents viewed July 14, 2008. Includes bibliographical references (p. 50-51).

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42

Wharry, Thomas Scott. "The synthesis of novel phosphonate and phosphinate inhibitors of proteinase enzymes." Thesis, Queen's University Belfast, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263577.

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43

Wilks, Helen M. "Design and synthesis of new enzymes on the lactate dehydrogenase framework." Thesis, University of Bristol, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.282796.

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44

Agyei-Owusu, Kwasi. "Synthesis and evaluation of novel inhibitors of thiamin diphosphate-dependent enzymes." Thesis, University of Cambridge, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.608816.

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45

Jareño, Cerulla Júlia. "Synthesis of Mn/Fe heterodimers as model complexes for heterobimetallic enzymes." Thesis, Uppsala universitet, Institutionen för kemi - Ångström, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-225957.

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46

Mutthamsetty, Vinay. "Design and Synthesis of Amino Acid-based Inhibitors Against Key Enzymes." University of Toledo / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=toledo1513014525316672.

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47

Storey, Benjamin 1973. "AQX : a novel gene in plant ubiquinone biosynthesis." Thesis, McGill University, 2002. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80882.

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C. elegans worms with mutations in the gene CLK-1 develop slowly and have an extended lifespan. CLK-1 encodes a mitochondrial protein that is responsible for the hydroxylation of 5-demethoxyubiquinone (DMQ), the penultimate step of ubiquinone (Coenzyme-Q or UQ) biosynthesis. Structural homologues of CLK-1 are found in mammals, fruit flies, yeast and some types of bacteria. Interestingly, however, there is no structural homologue of CLK-1 in the Arabidopsis genome and no plant homologue can be found in other sequence databases. Yeast with the CLK-1 homologue COQ7 deleted fail to grow on

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48

Morgan, C. R. "Properties and synthesis of the ribulose-1,5-biphosphate carboxylase large subunit binding protein." Thesis, University of Warwick, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.373050.

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49

Mitra, Adinpunya. "4-hydroxycinnamoyl-CoA hydratase/lyase from Pseudomonas fluorescens AN103 : characterisation and effects of expression in transformed root cultures of Datura stramonium." Thesis, University of East Anglia, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.301994.

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50

Parikh, Bhargiv. "Design, Synthesis and Characterization of D-glucosamine Low Molecular Weight Gelators." ScholarWorks@UNO, 2010. http://scholarworks.uno.edu/td/1110.

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Low molecular weight gelators (LMWGs) have gained much attention over the last few decades, because of their ability to form supramolecular architectures as well as their many potential applications in biomedical research and as advanced materials. Most of the gelators were discovered through serendipity, and their structural requirements are somewhat ambiguous. This is due, in part, to the fact that the supramolecular gelation phenomenon is not yet fully understood, though many structural classes have been found to be excellent organogelators. Carbohydrates are abundant natural resources that

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