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Rozprawy doktorskie na temat „Enzymes”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Ekici, Özlem Doğan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Available online, Georgia Institute of Technology, 2004:, 2003. http://etd.gatech.edu/theses/available/etd-04062004-164633/unrestricted/ekici%5Fozlem%5Fd%5F200312%5Fphd.pdf.

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Ekici, Ozlem Dogan. "Design, synthesis, and evaluation of novel irreversible inhibitors for caspases." Diss., Georgia Institute of Technology, 2003. http://hdl.handle.net/1853/5333.

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3

Müller, Roger. "Artificial enzymes: from catalytic antibodies toward de novo enzyme design /." Zürich : ETH, 2008. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=17897.

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4

Ghadge, G. D. "Microbial enzymes." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1986. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3251.

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5

Qian, Yuhui. "Study of Basic Wood Decay Mechanisms and Their Biotechnological Applications." Fogler Library, University of Maine, 2008. http://www.library.umaine.edu/theses/pdf/QianY2008.pdf.

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6

Zhao, Xueyan. "Nanoscale biocatalysts for bioelectrochemical applications." Akron, OH : University of Akron, 2006. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=akron1164149161.

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Thesis (M.S.)--University of Akron, Dept. of Chemical Engineering, 2006.<br>"December, 2006." Title from electronic thesis title page (viewed 06/27/2007) Advisor, Ping Wang; Committee members, Lu-Kwang Ju, Steven S. C. Chuang; Department Chair, Lu-Kwang Ju; Dean of the College, George K. Haritos; Dean of the Graduate School, George R. Newkome. Includes bibliographical references.

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7

Moore, Robert Goodwin Douglas C. "Towards the understanding of complex biochemical systems the significance of global protein structure and thorough parametric analysis /." Auburn, Ala, 2009. http://hdl.handle.net/10415/1766.

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8

Epstein, Todd Matthew. "Structural and kinetic studies of two enzymes catalyzing phospholipase A2 activity." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file 2.39 Mb., 186 p, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3200538.

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9

Kita, Keiko. "STUDIES ON NEW RESTRICTION ENZYMES AND MODIFICATION ENZYMES FROM BACTERIA." Kyoto University, 1989. http://hdl.handle.net/2433/78227.

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10

Ewing, Erin. "In vacuo glycation of enzymes: A novel approach for increasing enzyme stability." Thesis, University of Ottawa (Canada), 2006. http://hdl.handle.net/10393/27129.

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A novel approach for the thermostabilization of proteins was investigated. It is well established that proteins that are naturally highly glycosylated show an increased resistance to inactivation at high temperatures. However, non-enzymatic attachment of carbohydrate to proteins, otherwise known as protein glycation, under aqueous conditions is very difficult and has not been used as a general approach for increasing the thermostability of proteins. In the present study, advantage was taken of the in vacuo protein glycation procedure recently developed by Kaplan and his co-workers by which pro

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11

Banaszczyk, Mariusz G. "Artificial hydrolytic enzymes." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74289.

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The efficiencies of many rigidly held cis-aquahydroxotetraazacobalt(III) complexes in promoting the hydrolysis of phosphate esters (BDNPP, BNPP, NPP) have been compared. The phosphate diester bond in ((trpn)Co(OH)(BNPP)) $ sp{+}$ is hydrolyzed at about the same rate as BNPP bound to a real enzyme from Enterobacter aerogenes and about 10$ sp{10}$ times more rapidly than free BNPP. The dramatic increase in the activity of the Co(III) complex with change in the tetraamine ligand structure can be explained in terms of a detailed mechanism of the reaction.<br>Co(III) complexes, ((tren)Co(OH$ sb2)$(

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12

Harris, Rebecca Clare. "Polyketide synthase enzymes." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360886.

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13

Proctor, Mark Richard. "Carbohydrate modifying enzymes." Thesis, University of Newcastle Upon Tyne, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.416647.

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14

SILVA, JOSE A. A. da. "Aspectos de atividade biologica da giroxina (enzima trombina simile) isolada do veneno da cascavel brasileira, Crotalus durissus terrificus." reponame:Repositório Institucional do IPEN, 2004. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11191.

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Made available in DSpace on 2014-10-09T12:49:21Z (GMT). No. of bitstreams: 0<br>Made available in DSpace on 2014-10-09T14:02:30Z (GMT). No. of bitstreams: 1 09994.pdf: 3905779 bytes, checksum: 7cdc8d8ae9585729a6a818ed202c59c8 (MD5)<br>Dissertacao (Mestrado)<br>IPEN/D<br>Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP

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15

Reichstädter, Marek. "Imobilizace vybraných glykanohydroláz." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217152.

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The theoretical part of this thesis deals with cellulolytic enzymes, their microbial producers, the possibilities of using such enzymes in the industry and how can be enzymes - not only cellulolytic - immobilized. Experimental part examines the preparations created by immobilizing various amounts of the commercially used cellulolytic complex Cellulast 1.5L onto various synthetic carriers made of polyethylene terephthalate - commercially used Sorsilen, PET carrier and glutaraldehyde-treated PET carrier. Enzyme activity of these preparations was determined by Somogyi - Nelson method by spectroph

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16

Snider, Catherine E. "Synthesis and biochemical evaluation of irreversible inhibitors of aromatase /." The Ohio State University, 1986. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487266362338344.

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17

Silveira, Alvito J. "Synthesis of 6-guanidinobenzoxazinones as potential inhibitors of trypsin-like enzymes." Thesis, Georgia Institute of Technology, 1989. http://hdl.handle.net/1853/26914.

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18

Sun, An-Qiang. "How Do Enzymes Wear Out? Effects of Posttranslational Modifications on Structure and Stability of Proteins; The Triosephosphate Isomerase Model." Thesis, University of North Texas, 1991. https://digital.library.unt.edu/ark:/67531/metadc798116/.

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Triosephosphate isomerase (EC 5.3.1.1., TPI) undergoes specific posttranslational modifications (deamidation and oxidation) which are believed to initiate protein turnover by destabilization of the dimer. The crystal structures, amino acid sequences, and aging related changes of TPI from various species have been independently characterized by several laboratories. TPI has thus become the prototype enzyme for examining the initial steps in protein turnover. The binding of substrate enhances the specific deamidation of the mammalian enzyme, and a general mechanism of 'molecular wear and tear' [

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19

Haileselassie, Seble Sereke Berhan. "Production of enzyme-modified cheese and bioactive peptides by Lactobacillus and commercial enzymes." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp03/MQ50782.pdf.

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20

Lakay, Francisco Martin. "Fungal enzymes as animal feed additives." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52280.

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Thesis (MSc)--Stellenbosch University, 2001.<br>ENGLISH ABSTRACT: The use of fungal enzymes as ruminant feed digestibility enhancers was investigated. Currently, ruminants may not digest 38 to 80 % of fibrous forages' content. A renewed interest in the potential of feed enzymes for ruminants was prompted by the high costs of livestock production, together with the availability of newer enzyme preparations. Direct application of enzyme preparations can improve in vitro dry matter (DM) and neutral detergent fibre (NDF) degradation, indicating that direct-fed fibrolytic enzymes may be effect

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21

Karamohamed, Samer. "Pyrosequencing : enzymes and assays /." Stockholm : Department of Biotechnology, 1999. http://www.lib.kth.se/abs99/kara0923.pdf.

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22

Sule, Mohammed S. "Radiation inactivation of enzymes." Thesis, University of Salford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261991.

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23

Naylor, Neil J. "Biotransformations involving hydrolytic enzymes." Thesis, University of Warwick, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.263607.

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24

Banks, M. N. "Activators of lysosomal enzymes." Thesis, University of Manchester, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376247.

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25

Saldanha, Sanjay Adrian. "Studies on pantothenate enzymes." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620554.

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26

Mackay, Lindsey Gillian. "Porphyrin-based synthetic enzymes." Thesis, University of Cambridge, 1993. https://www.repository.cam.ac.uk/handle/1810/272641.

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27

LEE, KANG-MIN. "Enzymes en milieux heterogenes." Université Louis Pasteur (Strasbourg) (1971-2008), 1989. http://www.theses.fr/1989STR13051.

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L'emploi des enzymes en synthese organique pose quelque probleme du fait de la faible solubilite des produits organiques dans l'eau. C'est pourquoi l'alcool dehydrogenase a ete cristallisee; les microemulsions offrent des proprietes favorables a l'activite enzymatique

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28

Wirth, Petra. "Enzymes en solvants organiques." Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb37619244x.

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29

Vyas, P. R. "Studies on chitinolytic enzymes." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 1991. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/3012.

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30

Finnigan, William John Andrew. "The exploitation of thermophiles and their enzymes for the construction of multistep enzyme reactions from characterised enzyme parts." Thesis, University of Exeter, 2016. http://hdl.handle.net/10871/27323.

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Biocatalysis is a field rapidly expanding to meet a demand for green and sustainable chemical processes. As the use of enzymes for synthetic chemistry becomes more common, the construction of multistep enzyme reactions is likely to become more prominent providing excellent cost and productivity benefits. However, the design and optimisation of multistep reactions can be challenging. An enzyme toolbox of well-characterised enzyme parts is critical for the design of novel multistep reactions. Furthermore, while whole-cell biocatalysis offers an excellent platform for multistep reactions, we are

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31

Oudshoorn, Matthew Leslie. "Tests of predictions made by the Equilibrium Model for the effect of temperature on enzyme activity." The University of Waikato, 2008. http://hdl.handle.net/10289/2418.

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The Classical Model describing the effects of temperature on enzyme activity consists of two processes: the catalytic reaction defined by ΔG cat and irreversible inactivation defined by ΔG inact, this model however, does not account for the observed temperature- dependant behaviour of enzymes. The recent development of the Equilibrium Model is governed not only by ΔG cat and ΔG inact but also by two new intrinsic parameters ΔHeq and Teq, which describe the enthalpy and the temperature of the midpoint, respectively, of a active and reversibly inactive enzyme transition. Teq is central to the ph

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32

Garrett, Mark Denis. "A study on selectivity in microbial biotransformations of substituted arenes." Thesis, Queen's University Belfast, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.287620.

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33

Sha, Zhou. "A Chemical Approach to Detect and Characterize The Activities of Mitochondrial ATP-dependent Protease Lon and ClpXP." Case Western Reserve University School of Graduate Studies / OhioLINK, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=case1595239191845497.

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34

Fisher, Oriana. "Subcloning, enzymatic characterization, and in silico docking of transglutaminase 2." Waltham, Mass. : Brandeis University, 2009. http://dcoll.brandeis.edu/handle/10192/23253.

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35

Vögeli, Bastian [Verfasser], and Tobias [Akademischer Betreuer] Erb. "Control of reactive intermediates in enzymes and enzyme complexes / Bastian Vögeli ; Betreuer: Tobias Erb." Marburg : Philipps-Universität Marburg, 2019. http://d-nb.info/1185068716/34.

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36

Jordaan, Justin. "Isolation and characterization of a novel thermostable and catalytically efficient laccase from Peniophora sp. strain UD4." Thesis, Rhodes University, 2005. http://eprints.ru.ac.za/211/.

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37

Tsekoa, Tsepo L. "Structure, enzymology and genetic engineering of Bacillus sp. RAPc8 nitrile hydratase." Thesis, University of the Western Cape, 2005. http://etd.uwc.ac.za/index.php?module=etd&amp.

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Microbial nitrile hydratases are important industrial enzymes that catalyse the conversion of nitriles to the corresponding amides. A thermostable, cobalt-type Bacillus sp. RAPc8 microbial nitrile hydratase was cloned and expressed in E.coli. In this study the primary aim was to determine the molecular structure of Bacillus sp. RAPc8 microbial nitrile hydratase.

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38

Nourizad, Nader. "Recombinant Enzymes in Pyrosequencing Technology." Doctoral thesis, KTH, Biotechnology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3765.

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<p>Pyrosequencing is a DNA sequencing method based on thedetection of released pyrophosphate (PPi) during DNA synthesis.In a cascade of enzymatic reactions, visible light isgenerated, which is proportional to the number of nucleotidesincorporated into the DNA template. When dNTP(s) areincorporated into the DNA template, inorganic PPi is released.The released PPi is converted to ATP by ATP sulfurylase, whichprovides the energy to luciferase to oxidize luciferin andgenerate light. The excess of dNTP(s) and the ATP produced areremoved by the nucleotide degrading enzyme apyrase.</p><p>The commerci

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39

Basile, Lacy Jamel. "Cyanide-degrading enzymes for bioremediation." Texas A&M University, 2008. http://hdl.handle.net/1969.1/86035.

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Cyanide-containing waste is an increasingly prevalent problem in today's society. There are many applications that utilize cyanide, such as gold mining and electroplating, and these processes produce cyanide waste with varying conditions. Remediation of this waste is necessary to prevent contamination of soils and water. While there are a variety of processes being used, bioremediation is potentially a more cost effective alternative. A variety of fungal species are known to degrade cyanide through the action of cyanide hydratases, a specialized subset of nitrilases which hydrolyze cyanide to

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40

Ayixiamuguli, Nueraimaiti. "Lignin degradation using lignolytic enzymes." Thesis, University of Nottingham, 2016. http://eprints.nottingham.ac.uk/35262/.

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Lignin is the only plant biomass that contains aromatic groups in its structure and can provide a wide range of low molecular weight aromatic chemicals if its depolymerisation can be achieved successfully. Currently, lignin is mainly produced as a waste by-product by the paper and pulp industry and biorefineries. Therefore, the transformation of the phenolic-rich lignin into value added aromatic platform chemicals can be regarded of primary concern to improve the economic profitability of biorefining. Moreover, being a renewable resource, the consumption of fossil fuels will be reduced if lign

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41

Hammond, Richard Andrew. "Inducible enzymes in equine endotoxaemia." Thesis, Queen Mary, University of London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312910.

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42

Hirst, Judy. "Electron transport in redox enzymes." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364043.

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43

Kaw, Semiko. "Endothelin converting and degrading enzymes." Thesis, Oxford Brookes University, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.261235.

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44

Muroni, Maurizio. "Rational design of artificial enzymes." Thesis, Cardiff University, 2011. http://orca.cf.ac.uk/55043/.

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Despite the endeavours of many decades, the design of artificial enzymes remains challenging. The work presented here investigates two known molecules as scaffolds for the design of artificial enzymes an 18 amino acids a helical peptide with two disulfide bridges - 'Apoxaldie' able to catalyse the decarboxylation of oxaloacetate, and the 86-amino acid colicin E9 immunity protein (Im9) with four a helices. Apoxaldie was modified such that the active site lysines were substituted by 2,4-diaminobutyric acid in order to increase the proximity of the enzyme active site to the chiral environment of

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45

Whitaker, Richard George. "The electrochemistry of redox enzymes." Thesis, University of Warwick, 1989. http://wrap.warwick.ac.uk/4235/.

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The work presented in this thesis is of two types. Firstly methods for the electrochemical immobilisation of redox enzymes in organic polymers are described. The electrochemical monitoring of the immobilised enzyme reaction by detection of one of the enzyme's products is discussed, and the results obtained for such a system under a variety of experimental conditions are presented. A good understanding of the way in which such a system operates' was obtained by using a specially developed kinetic model., This model is explained fully in the theory chapter of this thesis. A variety of organic po

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46

Souter, Nicola H. "Thermophilic enzymes from Thermus ruber." Thesis, Heriot-Watt University, 1993. http://hdl.handle.net/10399/1437.

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47

Francis, Stewart. "Immunochemical detection of industrial enzymes." Thesis, Heriot-Watt University, 1996. http://hdl.handle.net/10399/701.

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48

O'Donnell, Raymond William. "Chitinolytic enzymes of Candida albicans." Thesis, University of Aberdeen, 1991. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=158392.

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It has been envisaged that lytic enzymes may be crucial in determining the morphology and growth of fungi, and may therefore represent a target for antifungal agents. The chitinolytic system of <i>Candida albicans</i> was investigated using a range of 4-methylumbelliferyl glycosides as model substrates, and its potential as a target for antibiotics has been assessed. Maximum hydrolysis was obtained with substrates having monomer and tetramer chain lengths, being attributed to <i>N</i>-acetylglucosaminidase and endochitinase respectively. Activities were investigated in cell fractions, vacuoles

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49

Doherty, Sean. "Apoplastic proteins, enzymes and radicals." Thesis, Durham University, 2000. http://etheses.dur.ac.uk/4376/.

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The soluble and readily extractable part of the plant extracellular matrix has been termed, the apoplast and contains a wide range of components such as, complex carbohydrates, structural proteins, enzymes and radicals that are known to be responsive to stress and developmental pressures. This thesis describes the development of a technique for the selective enrichment of apoplastic components for a range of subsequent analyses. Using this technique a number of apoplastic proteins were N-terminally sequenced and revealed 2 cell wall related enzymes, an antifungal protein and 3 auxin-binding/ge

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50

Ogilvie, Isla Marion. "Enzymes of mitochondrial #beta#-oxidation." Thesis, University of Newcastle Upon Tyne, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384937.

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