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Artykuły w czasopismach na temat "Fluorescent recovery after photobleaching (FRAP)"

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Cadar, Adrian G., Tromondae K. Feaster, Kevin R. Bersell, et al. "Real-time visualization of titin dynamics reveals extensive reversible photobleaching in human induced pluripotent stem cell-derived cardiomyocytes." American Journal of Physiology-Cell Physiology 318, no. 1 (2020): C163—C173. http://dx.doi.org/10.1152/ajpcell.00107.2019.

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Fluorescence recovery after photobleaching (FRAP) has been useful in delineating cardiac myofilament biology, and innovations in fluorophore chemistry have expanded the array of microscopic assays used. However, one assumption in FRAP is the irreversible photobleaching of fluorescent proteins after laser excitation. Here we demonstrate reversible photobleaching regarding the photoconvertible fluorescent protein mEos3.2. We used CRISPR/Cas9 genome editing in human induced pluripotent stem cells (hiPSCs) to knock-in mEos3.2 into the COOH terminus of titin to visualize sarcomeric titin incorporat
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Combs, Christian A., and Robert S. Balaban. "Enzyme-Dependant Fluorescence Recovery After Photobleaching (ED-FRAP): Application to Imaging Dehydrogenase Activity in Living Single Cells." Microscopy and Microanalysis 7, S2 (2001): 18–19. http://dx.doi.org/10.1017/s1431927600026167.

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Fluorescent recovery from photobleaching coupled with confocal microscopy was explored as a potential high-resolution method of imaging the distribution of enzyme activity in single living cardiac myocytes without relying on steady state measurements of fluorescence. On a fundamental level, much remains to be determined regarding how local conditions within a cell affect metabolism. Many studies have suggested that energy metabolism in muscle cells cannot be accurately described assuming a homogenous system of enzymatic reactions [1,2]. The autofluorescence of NADH has been used in many studie
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Srikantha, Nishanthan, Yurema Teijeiro-Gonzalez, Andrew Simpson, et al. "Determining vitreous viscosity using fluorescence recovery after photobleaching." PLOS ONE 17, no. 2 (2022): e0261925. http://dx.doi.org/10.1371/journal.pone.0261925.

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Purpose Vitreous humor is a complex biofluid whose composition determines its structure and function. Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules. The central pig vitreous is thought to closely match human vitreous viscosity. Diffusion is inversely related to viscosity, and diffusion is of fundamental importance for all biochemical reactions. Fluorescence Recovery After Photobleaching (FRAP) may provide a novel means of measuring intravitreal diffusion that could be applied to drugs and physiological macromolecu
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Braga, José, Joana M. P. Desterro, and Maria Carmo-Fonseca. "Intracellular Macromolecular Mobility Measured by Fluorescence Recovery after Photobleaching with Confocal Laser Scanning Microscopes." Molecular Biology of the Cell 15, no. 10 (2004): 4749–60. http://dx.doi.org/10.1091/mbc.e04-06-0496.

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Fluorescence recovery after photobleaching (FRAP) is a widely used tool for estimating mobility parameters of fluorescently tagged molecules in cells. Despite the widespread use of confocal laser scanning microscopes (CLSMs) to perform photobleaching experiments, quantitative data analysis has been limited by lack of appropriate practical models. Here, we present a new approximate FRAP model for use on any standard CLSM. The main novelty of the method is that it takes into account diffusion of highly mobile molecules during the bleach phase. In fact, we show that by the time the first postblea
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Orlova, Darya, Eva Bartova, Valeri Maltsev, and Kozubek Stanislav. "A non-fitting method employing a spatial sine window transform for inhomogeneous effective diffusion measurements by FRAP." Biophysical Journal 100, no. 2 (2011): 507–16. https://doi.org/10.1016/j.bpj.2010.11.080.

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Determining averaged effective diffusion constants from experimental measurements of fluorescent proteins in an inhomogeneous medium in the presence of ligand-receptor interactions poses problems of analytical tractability. Here, we introduced a nonfitting method to evaluate the averaged effective diffusion coefficient of a region of interest (which may include a whole nucleus) by mathematical processing of the entire cellular two-dimensional spatial pattern of recovered fluorescence. Spatially and temporally resolved measurements of protein transport inside cells were obtained using the fluor
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Lorén, Niklas, Joel Hagman, Jenny K. Jonasson, et al. "Fluorescence recovery after photobleaching in material and life sciences: putting theory into practice." Quarterly Reviews of Biophysics 48, no. 3 (2015): 323–87. http://dx.doi.org/10.1017/s0033583515000013.

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AbstractFluorescence recovery after photobleaching (FRAP) is a versatile tool for determining diffusion and interaction/binding properties in biological and material sciences. An understanding of the mechanisms controlling the diffusion requires a deep understanding of structure–interaction–diffusion relationships. In cell biology, for instance, this applies to the movement of proteins and lipids in the plasma membrane, cytoplasm and nucleus. In industrial applications related to pharmaceutics, foods, textiles, hygiene products and cosmetics, the diffusion of solutes and solvent molecules cont
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Golebiewska, Urszula, Jason G. Kay, Thomas Masters, et al. "Evidence for a fence that impedes the diffusion of phosphatidylinositol 4,5-bisphosphate out of the forming phagosomes of macrophages." Molecular Biology of the Cell 22, no. 18 (2011): 3498–507. http://dx.doi.org/10.1091/mbc.e11-02-0114.

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To account for the many functions of phosphatidylinositol 4,5-bisphosphate (PIP2), several investigators have proposed that there are separate pools of PIP2 in the plasma membrane. Recent experiments show the surface concentration of PIP2 is indeed enhanced in regions where phagocytosis, exocytosis, and cell division occurs. Kinases that produce PIP2 are also concentrated in these regions. However, how is the PIP2 produced by these kinases prevented from diffusing rapidly away? First, proteins could act as “fences” around the perimeter of these regions. Second, some factor could markedly decre
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Verma, Sanjay K., Pratibha Kumari, Shagufi Naz Ansari, Mohd Ovais Ansari, Dondinath Deori, and Shaikh M. Mobin. "A novel mesoionic carbene based highly fluorescent Pd(ii) complex as an endoplasmic reticulum tracker in live cells." Dalton Transactions 47, no. 44 (2018): 15646–50. http://dx.doi.org/10.1039/c8dt02778a.

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Wagner, Stefan, Simion Chiosea, Maria Ivshina, and Jeffrey A. Nickerson. "In vitro FRAP reveals the ATP-dependent nuclear mobilization of the exon junction complex protein SRm160." Journal of Cell Biology 164, no. 6 (2004): 843–50. http://dx.doi.org/10.1083/jcb.200307002.

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We present a new in vitro system for characterizing the binding and mobility of enhanced green fluorescent protein (EGFP)–labeled nuclear proteins by fluorescence recovery after photobleaching in digitonin-permeabilized cells. This assay reveals that SRm160, a splicing coactivator and component of the exon junction complex (EJC) involved in RNA export, has an adenosine triphosphate (ATP)–dependent mobility. Endogenous SRm160, lacking the EGFP moiety, could also be released from sites at splicing speckled domains by an ATP-dependent mechanism. A second EJC protein, RNPS1, also has an ATP-depend
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Wadsworth, P., and E. D. Salmon. "Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching." Journal of Cell Biology 102, no. 3 (1986): 1032–38. http://dx.doi.org/10.1083/jcb.102.3.1032.

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One recent hypothesis for the mechanism of chromosome movement during mitosis predicts that a continual, uniform, poleward flow or "treadmilling" of microtubules occurs within the half-spindle between the chromosomes and the poles during mitosis (Margolis, R. L., and L. Wilson, 1981, Nature (Lond.), 293:705-711). We have tested this treadmilling hypothesis using fluorescent analog cytochemistry and measurements of fluorescence redistribution after photobleaching to examine microtubule behavior during metaphase of mitosis. Mitotic BSC 1 mammalian tissue culture cells or newt lung epithelial cel
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Rozprawy doktorskie na temat "Fluorescent recovery after photobleaching (FRAP)"

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Gaffield, Michael A. "FRAP measurements of synaptic vesicle mobility in motor nerve terminals /." Connect to abstract via ProQuest. Full text is not available online, 2007.

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Thesis (Ph.D. in Neuroscience) -- University of Colorado Denver, 2007.<br>Typescript. Includes bibliographical references (leaves 84-93). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;
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Rodriguez-Enriquez, Ricardo. "Analysis of Bcl-2 family protein interactions in live cells by fluorescence recovery after photobleaching." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/analysis-of-bcl2-family-protein-interactions-in-live-cells-by-fluorescence-recovery-after-photobleaching(aa5eb271-6e43-48f3-940d-f63763ea4629).html.

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The Bcl-2 family of proteins strictly regulates the intrinsic pathway of apoptosis. Direct physical interactions between Bcl-2 proteins regulate mitochondrial outerpermeabilisation (MOMP), which occurs in response to various cell stresses andapoptotic stimuli. How changes in Bcl-2 protein activity regulate apoptosiscommitment is still unclear, especially with regard to how they interact with eachother within the context of the mitochondrial membrane. Recent studies haveshown that Bcl-2 proteins exist in a dynamic equilibrium between the mitochondriaand the cytosol. In this thesis, by using FRA
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Innhausen, u. Knyphausen Adrian zu [Verfasser], and Ralph [Akademischer Betreuer] Rupp. "A novel method for Fluorescence Recovery after Photobleaching (FRAP) analysis of chromatin proteins in pluripotent embryonic cells of the South African clawed frog X. laevis / Adrian zu Innhausen u. Knyphausen ; Betreuer: Ralph Rupp." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/1221960563/34.

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Equy, Eloïse. "Polymersomes Janus : conception rationnelle, préparation et fonctionnalisation asymétrique pour le développement de systèmes auto-propulsés de délivrance ciblée de médicaments." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0465.

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Mimer les propriétés des cellules vivantes dans des protocellules artificielles suscite un intérêt considérable, notamment pour reproduire la motilité et le mouvement directionnel dans des applications de thérapies « intelligentes ». En raison de leur morphologie vésiculaire et de leur stabilité, les polymersomes présentent un grand potentiel pour la délivrance de médicaments, et l'introduction d'une asymétrie est essentielle pour permettre leur auto-propulsion. Bien que plusieurs approches, telles que la séparation de phase au sein de la membrane, aient été utilisées pour créer des polymersom
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Irrechukwu, Onyi Nonye. "Role of matrix composition and age in solute diffusion within articular cartilage." Diss., Atlanta, Ga. : Georgia Institute of Technology, 2007. http://hdl.handle.net/1853/19699.

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Thesis (Ph.D)--Biomedical Engineering, Georgia Institute of Technology, 2008.<br>Committee Chair: Levenston, Marc; Committee Member: Garcia, Andres; Committee Member: Koros, William; Committee Member: Sambanis, Athanassios; Committee Member: Temenoff, Johnna; Committee Member: Vidakovic, Brani.
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Piette, Nathalie. "Micropatterning subcellulaire pour étudier la connectivité neuronale." Electronic Thesis or Diss., Bordeaux, 2024. http://www.theses.fr/2024BORD0034.

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L'impression protéique a initialement été utilisée pour reproduire et comprendre l’influence de la matrice extracellulaire sur les cellules et certains de leurs composants. Au cours de la dernière décennie, l'impression subcellulaire s’est développée, permettant d’étudier les interactions protéiques et leur rôle dans les voies de signalisation ainsi que dans la formation de synapses, immunologiques ou neuronales.La connexion synaptique est médiée par les protéines d’adhésion synaptique présentes de chaque côté de la synapse. En raison de la complexité de l’environnement synaptique mais égaleme
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Części książek na temat "Fluorescent recovery after photobleaching (FRAP)"

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Saito, Takumi, Daiki Matsunaga, and Shinji Deguchi. "Long-Term Fluorescence Recovery After Photobleaching (FRAP)." In Methods in Molecular Biology. Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2851-5_21.

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Carnell, Michael, Alex Macmillan, and Renee Whan. "Fluorescence Recovery After Photobleaching (FRAP): Acquisition, Analysis, and Applications." In Methods in Molecular Biology. Springer New York, 2014. http://dx.doi.org/10.1007/978-1-4939-1752-5_18.

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Giakoumakis, Nickolaos Nikiforos, Maria Anna Rapsomaniki, and Zoi Lygerou. "Analysis of Protein Kinetics Using Fluorescence Recovery After Photobleaching (FRAP)." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6810-7_16.

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van Royen, Martin E., Pascal Farla, Karin A. Mattern, Bart Geverts, Jan Trapman, and Adriaan B. Houtsmuller. "Fluorescence Recovery After Photobleaching (FRAP) to Study Nuclear Protein Dynamics in Living Cells." In The Nucleus. Humana Press, 2008. http://dx.doi.org/10.1007/978-1-60327-461-6_20.

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Takeshi, Shimi, Chan-Gi Pack, and Robert D. Goldman. "Analyses of the Dynamic Properties of Nuclear Lamins by Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS)." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3530-7_5.

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Warrington, Samantha J., Helen Strutt, and David Strutt. "Use of Fluorescence Recovery After Photobleaching (FRAP) to Measure In Vivo Dynamics of Cell Junction–Associated Polarity Proteins." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2035-9_1.

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Badrinarayanan, Anjana, and Mark C. Leake. "Using Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex In Vivo." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3631-1_4.

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Badrinarayanan, Anjana, and Mark C. Leake. "Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex in Live Escherichia coli Bacteria." In Methods in Molecular Biology. Springer US, 2022. http://dx.doi.org/10.1007/978-1-0716-2221-6_4.

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Caydasi, Ayse Koca, and Gislene Pereira. "Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP)." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6502-1_13.

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Brown, Edward, Ania Majewska,, and Rakesh K. Jain. "Photobleaching and Recovery with Nonlinear Microscopy." In Handbook of Biomedical Nonlinear Optical Microscopy. Oxford University PressNew York, NY, 1998. http://dx.doi.org/10.1093/oso/9780195162608.003.0026.

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Abstract Fluorescence recovery after photobleaching (FRAP), or fluorescence photobleaching recovery (FPR), describes a family of related techniques that measure transport properties of fluorescently labeled molecules. This is done by monitoring the evolution of a fluorescence signal after a spatially localized population of fluorophores is bleached by light. The classical FRAP experiment with one-photon excitation uses a focused laser beam to bleach a disk-shaped region of fluorescently labeled molecules in a thin sample such as a cell membrane or a lamellipodium (Fig. 26.1) (Axelrod et al., 1
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Streszczenia konferencji na temat "Fluorescent recovery after photobleaching (FRAP)"

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Cao, Ziyi, Dustin Harmon, Jiayue Rong, Andreas Geiger, and Garth J. Simpson. "Fourier-transform Fluorescence Recovery after Photobleaching (FT-FRAP) diffusion imaging analysis." In Advanced Chemical Microscopy for Life Science and Translational Medicine 2022, edited by Garth J. Simpson, Ji-Xin Cheng, and Wei Min. SPIE, 2022. http://dx.doi.org/10.1117/12.2607631.

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BIRMINGHAM, J. J. "PHASE-FRAP: A NEW FREQUENCY-DOMAIN VARIANT OF FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING." In Proceedings of the Fifth Royal Society–Unilever Indo-UK Forum in Materials Science and Engineering. A CO-PUBLICATION OF IMPERIAL COLLEGE PRESS AND THE ROYAL SOCIETY, 2000. http://dx.doi.org/10.1142/9781848160163_0007.

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Docimo, Jennifer E., and John E. Novotny. "Measuring Diffusion in Non-Sectioned Articular Cartilage: A FRAP Sensitivity Study." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192375.

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Measuring the diffusion of molecules within articular cartilage is essential in characterizing its behavior. Information about this important mechanism may be useful to understanding changes in cartilage during degeneration or osteoarthritis. One method used in quantifying diffusion is fluorescence recovery after photobleaching (FRAP). The FRAP technique has been used in previous studies for cartilage [1] and various tissues [2]. In FRAP, a small region of interest (ROI) is selected within the tissue and fluorescent molecules are bleached using a higher laser power than would be used for imagi
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Geiger, Andreas C., Casey J. Smith, and Garth J. Simpson. "Multi-photon excited Fourier-transform fluorescence recovery after photobleaching (FT-FRAP) with patterned illumination." In Multiphoton Microscopy in the Biomedical Sciences XX, edited by Ammasi Periasamy, Peter T. So, and Karsten König. SPIE, 2020. http://dx.doi.org/10.1117/12.2545908.

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Oubekka, S. Daddi, R. Briandet, F. Waharte, M. P. Fontaine-Aupart, and K. Steenkeste. "Image-based Fluorescence Recovery After Photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms." In European Conference on Biomedical Optics. OSA, 2011. http://dx.doi.org/10.1364/ecbo.2011.80871i.

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Daddi Oubekka, S., R. Briandet, F. Waharte, M. P. Fontaine-Aupart, and K. Steenkeste. "Image-based fluorescence recovery after photobleaching (FRAP) to dissect vancomycin diffusion-reaction processes in Staphylococcus aureus biofilms." In European Conferences on Biomedical Optics, edited by Nirmala Ramanujam and Jürgen Popp. SPIE, 2011. http://dx.doi.org/10.1117/12.889461.

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Simpson, Garth J. "Imaging of molecular mobility by spatial Fourier transform fluorescence recovery after photobleaching (FT-FRAP) with structured illumination." In Advanced Chemical Microscopy for Life Science and Translational Medicine 2024, edited by Garth J. Simpson, Ji-Xin Cheng, and Wei Min. SPIE, 2024. http://dx.doi.org/10.1117/12.3005854.

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Teijeiro Gonzalez, Yurema, Klaus Suhling, Andrew Beavil, et al. "Fluorescence Recovery After Photobleaching (FRAP) with simultaneous Fluorescence Lifetime and time-resolved Fluorescence Anisotropy Imaging (FLIM and tr-FAIM)." In Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, edited by Thomas G. Brown and Tony Wilson. SPIE, 2019. http://dx.doi.org/10.1117/12.2508692.

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Cao, Ziyi, Dustin M. Harmon, Ruochen Yang, et al. "Diffusion mapping by Fourier-transform fluorescence recovery after photobleaching (FT-FRAP) for phase separation in drug formulations (Conference Presentation)." In Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, edited by Garth J. Simpson, Ji-Xin Cheng, and Wei Min. SPIE, 2023. http://dx.doi.org/10.1117/12.2648473.

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Albro, Michael B., Vikram Rajan, Clark T. Hung, and Gerard A. Ateshian. "Fickian Behavior and Concentration-Dependence of the Diffusion of Dextran in Agarose." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176646.

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Various studies have attempted to quantify the effects of loading on nutrient transport in cartilage and other soft tissues. The application of a dynamic mechanical stimulus has been shown to significantly enhance the mechanical properties of chondrocyte-seeded agarose [1]. While the mechanism for this enhancement is still not completely understood, dynamic loading has been shown theoretically [2] as well as experimentally [3] to increase the uptake of large molecules. Since dextran is available in a wide range of molecular weights and can be conjugated with fluorphores, it has become a popula
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