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Ibrahim, Arsyik, M. Arifuddin, Wisnu Cahyo P, Wahyu Widayat, and Mahfuzun Bone. "ISOLATION, CHARACTERIZATION AND SECONDARY METABOLITE ENDOPHYTIC FUNGAL ISOLATE FROM PERONEMA CANESCENS JACK LEAF AND COPTOSAPELTA TOMENTOSA VAL. K. HEYNE ROOT." Journal of Tropical Pharmacy and Chemistry 4, no. 5 (2019): 215–25. http://dx.doi.org/10.25026/jtpc.v4i5.169.

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Has been done Isolation, Characterization and Secondary Metabolite Endophytic Fungal Isolate from Peronema canescens Jack Leave and Coptosapelta tomentosa Valeton K. Heyne Root. The aim of this research is to know the number of fungal isolates, chromatogram profile and secondary metabolite group of endophytic fungal isolates from P. canencens leaves and C. tomentosa root. Characterization of endophytic fungal isolates was done macroscopically and microscopically. Identification of secondary metabolites endophytic fungal isolates were performed by chemical reaction test and TLC (Thin Layer Chro
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Manguilimotan, Lebeth C., and Jayzon G. Bitacura. "Biosorption of Cadmium by Filamentous Fungi Isolated from Coastal Water and Sediments." Journal of Toxicology 2018 (October 22, 2018): 1–6. http://dx.doi.org/10.1155/2018/7170510.

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The use of microorganisms in decontaminating the environment encumbered with heavy metal pollutants through biosorption is considered as a good option for bioremediation. This study was conducted to isolate Cadmium (Cd) tolerant fungi from coastal waters and sediments, compare their biosorption capabilities, and identify the isolates with the highest Cd uptake. Water and sediment samples were collected near the effluent sites of industrial belt in Ibo, Lapu-lapu City, Cebu, Philippines. Potato dextrose agar (PDA) plates containing Cd (25, 50, 75, and 100 ppm) were used to isolate Cd tolerant f
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Jiotode, Vaishnavi A., Chandrakant T. Kumbhar, Ashok C. Jadhav, et al. "Studies on Cellulolytic Fungal Flora from Forests of Maharashtra, India." International Journal of Environment and Climate Change 14, no. 2 (2024): 993–1001. http://dx.doi.org/10.9734/ijecc/2024/v14i24012.

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The present investigation was conducted to isolate effective cellulolytic fungal flora from soil samples collected from various forests in Maharashtra (India). The study aimed to determine hydrolytic zones and relative enzyme activity for selecting most efficient fungal isolates. Further, the isolates were identified using morphological identification technique. Altogether 137 fungal isolates were obtained in the present study, collected from forests of Lonavala, Chandrapur, Mahabaleshwar, Gadchiroli and Bhandardara. The screening of these fungal isolates for cellulase production revealed that
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Michael, Michael, Nampiah Sukarno, Sofi Mursidawati, Edhi Sandra, and Nadiya Dwi Rahayu. "Isolasi dan Identifikasi Cendawan Endofit Akar Anggrek Epifit dan Hemiepifit." Jurnal Sumberdaya Hayati 9, no. 4 (2023): 152–63. http://dx.doi.org/10.29244/jsdh.9.4.152-163.

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Orchids form a symbiotic relationship with endophytic fungi throughout their life cycle, including their seed germination. Research on orchid endophytic fungi in Indonesia is still limited. This study aimed to isolate and identify endophytic fungi of Phalaenopsis amabilis, Dipodium brevilabium, Vanilla planifolia, and Aerides odorata orchids. Endophytic fungi were isolated from surface-sterilized orchid root pelotons. Fungal identification was based on morphological and molecular characteristics using ITS rDNA sequences, followed by BLAST and phylogenetic tree analysis. All orchids studied had
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Hossain, Sk Amir, Toma Sharma, and Rima Akter. "Screening and Characterization of Potential Fungi from Environmental Samples for Extracellular Phytase Production." Journal of Advances in Biology & Biotechnology 27, no. 10 (2024): 1131–43. http://dx.doi.org/10.9734/jabb/2024/v27i101535.

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Phytases are enzymes of significant interest due to their ability to hydrolyze phytic acid, releasing inorganic phosphate that is essential for various biological processes. This study aimed to screen and characterize potential phytase-producing fungi isolated from environmental samples for extracellular phytase production. Various environmental samples were collected from different locations in the Khulna region. A total of 15 fungal isolates were obtained, and morphological and microscopic characterization aided in the preliminary identification of these fungal species. Screening for extrace
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AJIJOLAKEWU, Kamoldeen Abiodun, Alhasan SANI, Ganiyu P. OYEYIOLA, et al. "Cellulase Production Potentials of the Microbial Profile of Some Sugarcane Bagasse Dumping Sites in Ilorin, Nigeria." Notulae Scientia Biologicae 5, no. 4 (2013): 445–49. http://dx.doi.org/10.15835/nsb549176.

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This research work investigated cellulase production potentials of the microbial profile of three sugarcane bagasse dumping sites at Zango area, Ilorin, Nigeria. The microbial isolates were screened for cellulase production with a view to select the best organism for eventual cellulase production. Pour Plate method was used for the isolation and a total of thirteen (13) different organisms including both fungal and bacterial species were isolated and screened. Six (6) fungal isolates identified as Mucor racemosus, Aspergillus niger, Aspergillus flavus, Neurospora sitophilus, Penicillium oxalic
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Kasambala Donga, Trust, Richard Meadow, Nicolai V. Meyling, and Ingeborg Klingen. "Natural Occurrence of Entomopathogenic Fungi as Endophytes of Sugarcane (Saccharum officinarum) and in Soil of Sugarcane Fields." Insects 12, no. 2 (2021): 160. http://dx.doi.org/10.3390/insects12020160.

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The natural occurrence of entomopathogenic fungal endophytes in sugarcane (Saccharum officinarum) and in soil samples from sugarcane fields was evaluated in Chikwawa District, southern Malawi. Fungi from soil were isolated by baiting using Galleria mellonella larva. Fungal endophytes were isolated from surface-sterilized plant tissue sections. Forty-seven isolates resembled the genus Beauveria, 9 isolates were Metarhizium, and 20 isolates were Isaria. There was no significant difference in the number and type of fungal isolates collected from soil and from plant tissue. There was, however, a s
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Khastini, Rida Oktorida, Nampiah Sukarno, Utut Widyastuti Suharsono†, and Yasuyuki Hashidoko†. "Isolasi dan Respons Tumbuh Cendawan Mutualistik Akar pada Beberapa Tanaman Pangan dan Kehutanan." Jurnal Ilmu Pertanian Indonesia 27, no. 1 (2022): 85–94. http://dx.doi.org/10.18343/jipi.27.1.85.

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The study aims to isolate and test the effectiveness of mutualistic root symbiont fungi isolates from the roots of rubber plants grown in marginal acidic soil plantations in increasing the growth of food crops and forestry plants. The fungal were isolated by root surface sterilization methods. We obtained 19 fungal isolates consisting of 8 genera, namely Alternaria, Aspergillus, Cladosporium, Curvularia, Fusarium, Penicillium Paecilomyces, Trichoderma, and mycelia sterilia. All isolates were subjected to a pathogenicity test on the Centrosema pubescens plant. Five out of the 19 fungal isolates
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YIN, WONG KAH, NUR ELIA NADHIRA MOHD ASMADI, NURUL FAZIHA IBRAHIM, and SUHAIZAN LOB. "IDENTIFICATION OF FUNGAL ISOLATES ASSOCIATED WITH ANTHRACNOSE DISEASE ON CHILLI AND ITS PATHOGENIC LEVEL." Universiti Malaysia Terengganu Journal of Undergraduate Research 2, no. 3 (2022): 51–56. http://dx.doi.org/10.46754/umtjur.v2i3.164.

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Many chilli producers in Malaysia are facing a huge problem of anthracnose disease that infect the chilli fruits either at pre- or post-harvest stage. Due to this infection, the chilli fruits become rotten and could be colonized by grey conidial masses which is unfit for human consumption and cannot be sold. This study was aimed to isolate the fungal species associated with anthracnose symptoms collected from several mini-markets near Gong Badak, Terengganu. Pathogenicity test was conducted for all these isolates to evaluate their pathogenic level. Based on the results, five fungal isolates we
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Dayok, OLUKEMI, C. M. Z. Whong, I. O. Abdullahi, and B. D. Kashina. "Characterization of Fungi Inducing Post-Harvest Deterioration and the Factors Predisposing Tomato Fruits to Fungal Induced Spoilage in Zaria, Nigeria." UMYU Journal of Microbiology Research (UJMR) 9, no. 1 (2024): 192–201. http://dx.doi.org/10.47430/ujmr.2491.021.

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This study was carried out to characterize fungi-inducing post-harvest deterioration of tomato fruits and the factors predisposing the tomato fruits to fungal-induced spoilage in Zaria, Nigeria. A total of two hundred and twenty-five spoilt tomatoes of three different cultivars were collected using guided random selection (45 each) from five markets (Dakachi, Danmangaji, Sabon-gari, Samaru, and Basawa) in Zaria, Nigeria. The fungal isolates were isolated using the standard mycological method. The cultural and microscopic characterization of fungi isolated revealed eleven genera belonging to Fu
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Verma, Shiv Kumar, Anand Kumar, Moti Lal, and Mira Debnath. "Biodegradation of Synthetic Dye by Endophytic Fungal Isolate in Calotropis procera Root." International Journal of Applied Sciences and Biotechnology 3, no. 3 (2015): 373–80. http://dx.doi.org/10.3126/ijasbt.v3i3.13136.

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In this study, based on colony morphology characteristics, a total of 19 fungal endophytes were isolated from root of Calotropis Procera a traditional Indian medicinal plant. All fungal isolates were screened for their dye degradation ability. The dyes used as test dyes were Rose Bengal (RB), azo dye Methyl Red (MR), Coomassie Brilliant Blue (CBB) and Methylene Blue (MB) and the concentration of each dye in the experiment was kept 100mg/L. Among the 19 fungal endophytic isolates (CPR1-CPR19), only one isolate CPR4 showed strong dye decolourization capability against all the four test dye. Dye
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Narayanan, MiniPalathingal. "Fungal isolates 2014." Journal of The Academy of Clinical Microbiologists 17, no. 1 (2015): 36. http://dx.doi.org/10.4103/0972-1282.158806.

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Muhtarima, Jannat, Farzana Haque Tumpa, Md Rashidul Islam, and Md Atiqur Rahman Khokon. "Pathogenic variability of Colletotrichum sp. from chilli anthracnose and their tolerance to carbendazim." Journal of the Bangladesh Agricultural University 16, no. 3 (2018): 417–23. http://dx.doi.org/10.3329/jbau.v16i3.39411.

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Twelve isolates of Colletotrichum capsici and Colletotrichum gloeosporides were isolated from anthracnose infected chilli fruits from different areas of Mymensingh. Isolated pure fungal isolates were grouped on the basis of their morphological characters viz. colony color and compactness, size, shape and number of conidia. The white colored isolates were identified as C. capsici and showed faster growth on PDA medium. In contrast, grey colored fungal isolates were identified as C. gloeosporides and comparatively slow growth on PDA medium. All the fungal isolates were pathogenically active and
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Slemmons, Caleb, Gregory Johnson, and Laurie B. Connell. "Application of an automated ribosomal intergenic spacer analysis database for identification of cultured Antarctic fungi." Antarctic Science 25, no. 1 (2012): 44–50. http://dx.doi.org/10.1017/s0954102012000879.

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AbstractWe utilized an automated ribosomal intergenic spacer analysis (ARISA) method as a more rapid alternative to classical morphological/nutritional identification and a less expensive alternative to sequencing for identification and grouping of isolates in culture-based fungal abundance studies. This method is well suited for the study of culturable Antarctic soil fungal communities where both abundance and diversity are relatively low. We optimized template concentration and verified the effect of primer selection from eight commonly used fungal polymerase chain reaction primers on ARISA
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Noori, Aya Fadhil, and Rafea Zeidan Mukhlif. "Molecular Profiling of Selected Aspergillus Species Parasitizing Stored Wheat Seeds in the Silos of Tikrit City, Iraq." International Journal of Environmental Sciences 11, no. 1s (2025): 88–96. https://doi.org/10.64252/ng21xk48.

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This study aimed to isolate and identify fungal species.AspergillusFrom wheat seeds stored in silos in Tikrit city, Salah al-Din Governorate, to assess the risks of fungal contamination on the safety of stored crops. Samples were collected from two local wheat seed varieties (with and without sorghum), and fungi were isolated from them using culture techniques on medium.PDA. Fungal isolates were identified based on morphological characteristics (colony color, texture, conidiophore and conidia shape), in addition to molecular identification using DNA sequencing of the ITS region of rRNA, after
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Bendangsenla, Susanta Banik, N. Tiameren Ao, et al. "Morphological Characterization of Fungal Endophytes Isolated from the Leaves and Roots of Banana Plants (Musa sp.)." Microbiology Research Journal International 35, no. 3 (2025): 72–81. https://doi.org/10.9734/mrji/2025/v35i31552.

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In recent years, fungal endophytes are being studied extensively for their crucial role in plants growth promotion as well as their role in antagonistic activity against many plant pathogens. In the present investigation, focus was made to study fungal endophytes, to isolate and morphologically characterized and identify from the leave and roots of wild and locally cultivated bananas from the Chumoukedima district, state Nagaland, India. Altogether 58 fungal endophytes were isolated and out of these, 43 isolates were from the leave samples and 15 isolates from the root samples. Out of these, 5
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Iyanyi, N. G., A. E. Ataga, I. S. Rotimi, and I. Blessing. "Molecular identification of fungi associated with avocado (Persea americana Mill.) fruits." Agro-Science 20, no. 1 (2021): 80–86. http://dx.doi.org/10.4314/as.v20i1.13.

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Avocado (Persea americana Mill.) is grown for its nutritious fruit. However, the quantity and quality of these fruits are threatened by some fungal organisms which can cause health complications when it is consumed by man. DNA extraction provides a unique tool for identification of organisms. This study was conducted to isolate and identify fungal species associated with avocado fruit using both morphological and molecular techniques. Fungal species were isolated from Persea americana purchased from Choba market, Port Harcourt, Rivers State, Nigeria using Potato Dextrose Agar (PDA) as a growth
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Elfiati, D., Delvian, M. Syafitri, and A. Hidayat. "Exploring endophytic fungi from Rhizophora spp leaves for improving plant growth." IOP Conference Series: Earth and Environmental Science 977, no. 1 (2022): 012018. http://dx.doi.org/10.1088/1755-1315/977/1/012018.

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Abstract Endophytic fungi are a group of fungi inhabiting the plant tissue for part or all of their life cycle without exposing any hazard to the host. Endophytic fungi can produce bioactive chemicals or secondary metabolites in proportion to their host plant, therefore no parts of the plant are needed to obtain these compounds. As a result, the survivability of a plant survival is maintained. The aim of this study was to obtain endophytic fungi isolated from the leaves of Rhizophora mucronata and Rhizophora apiculata. R. mucronata and R. apiculata leaves were collected at random in Lubuk Kert
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Bhardwaj, Anupama, Sonia Chaman, and Sangeeta Verma. "PRODUCTION OF ANTIBACTERIAL AGENT FROM FUNGI ISOLATED FROM PHARMACEUTICAL SOIL SAMPLE BY FERMENTATION UNDER OPTIMIZED CONDITIONS." Asian Journal of Pharmaceutical and Clinical Research 10, no. 7 (2017): 110. http://dx.doi.org/10.22159/ajpcr.2017.v10i7.18258.

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Objective: This study aimed to isolate fungi having antibacterial activity from pharmaceutical site soil sample and production of antibacterial agents by solid and submerged state fermentation under optimized conditions. Antibacterial activity of laboratory isolated and produced antibacterial agent was compared with other commercialized antibiotics to check the efficiency of laboratory produced antibacterial agent. Methods: For isolation and characterization of fungal isolates American Public Health Association standard was followed. Antibacterial activity was determined using disc diffusion a
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Ghafouri, H. K., and Y. N. Alhamiri. "Detection of Aflatoxin B1-producing Aspergillus flavus in Fish Feed and its Effects on Fish Farming and Health in Iraq." IOP Conference Series: Earth and Environmental Science 1449, no. 1 (2025): 012048. https://doi.org/10.1088/1755-1315/1449/1/012048.

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Abstract The research aims to conduct an environmental and biological survey of the level of contamination in fish feed and stored grains used in their production by dangerous fungi capable of producing aflatoxin toxins. Evaluation of the impact of aflatoxin B1 pollution levels on the breeding, production, and health of fish in Iraq. The survey results revealed contamination of all samples studied with various fungal isolates, focusing on the biological diversity of A. flavus isolates. A total of 802 fungal isolates belonging to this species were isolated and identified, which were the most pr
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Al-Shindah, Raghad S. D., Abdullah A. Hassan, and Muhammad S. Mansour. "Isolation and Identification of Entomopathogenic Fungi from of Green Peach Aphid Myzus Persicae and Evaluation of Their Activity for Insect Control." IOP Conference Series: Earth and Environmental Science 1060, no. 1 (2022): 012093. http://dx.doi.org/10.1088/1755-1315/1060/1/012093.

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Abstract Twenty-one different fungal isolates were isolated from samples of infected insects from different areas in Salah El-Din Governorate. A preliminary screening of the isolated fungi was carried out using different stages of green peach insect for the purpose of estimating its pathogenicity. The results showed that the fungal spores suspension and fungal filtrates of isolates (RA-4, RA-5, RA-9, RA-10, RA-12, RA-13, RA-14, RA-16, RA-23, RA-24) significantly superior on other isolates in killing green peach insects, the isolate (RA-5) achieved the highest mortality rate of 95.3% during the
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Iyany, N. G., and A. E. Ataga. "Isolation and Molecular Identification of some Fungi Associated with Jatropha curcas (L.)." Nigerian Journal of Biotechnology 37, no. 2 (2021): 103–12. http://dx.doi.org/10.4314/njb.v37i2.10.

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Jatropha curcas is a plant of great economic importance that experiences high incidence of fungal attack. Misidentification of the fungal species is bound to occur with the use of traditional cultural methods where organisms are identified morphologically and/or microscopically. This study was carried out to isolate and identify the fungi associated with Jatropha curcas (L.) using both traditional/ cultural techniques and molecular methods. The fungi were isolated from diseased leaves and stems of J. curcas using both Standard Blotter and Potato Dextrose Agar (PDA) methods. DNA was extracted f
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Sidhiawan, Anak Agung Putu, Sukiman Sukiman, Sarkono Sarkono, Ernin Hidayati, and Bambang Fajar Suryadi. "Eksplorasi Potensi Bacillus spp. sebagai Bakteri Pemacu Pertumbuhan Tanaman di Hutan Primer Resort Kembang Kuning." Bioscientist : Jurnal Ilmiah Biologi 11, no. 2 (2023): 1017. http://dx.doi.org/10.33394/bioscientist.v11i2.8403.

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Kembang Kuning resort is one of the Mount Rinjani National Park Resorts that have high biodiversity, so it has the potential for plant growth-promoting bacteria. Bacillus sp. is one species of bacteria that has great potential as a plant growth-promoting bacteria. The purpose of this study was to isolate and determine the ability of phosphate solubilization, nitrogen-fixing and anti-fungal activity of Bacillus spp. isolated from the Primary Forest of Kembang Kuning Resort, Gunung Rinjani National Park. This research is an exploratory study consisting of the isolation of Bacillus sp., phosphate
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MOHD ASMADI, NUR ELIA NADHIRA, WONG KAH YIN, NUR HADINA SALEH, NURUL FAZIHA IBRAHIM, and SUHAIZAN LOB. "FUNGAL SPECIES ASSOCIATED WITH BLACK SPOT DISEASE IN ROSE." Universiti Malaysia Terengganu Journal of Undergraduate Research 2, no. 3 (2020): 45–50. http://dx.doi.org/10.46754/umtjur.v2i3.162.

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Black spot disease is a significant worldwide disease on the rose plant. Due to this infection, the leaves become yellow and eventually fall off. The occurrence of this disease has become a major problem, especially in landscape purpose. Therefore, this research was conducted to isolate fungal species from black spot disease in rose and identify using morphological characteristics. Then, all the isolates were tested for pathogenicity to confirm Koch’s postulates. In this study, four fungal isolates have been successfully isolated from black spot disease in rose namely Rhizoctonia sp. (one isol
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Basha, Maha A., Yousseria M. Shetaia, Fathy M. Mehaya, and Fatma H. Abdelzaher. "Solid-state fermentation and optimization of cellulase production using local fungal isolate." Egyptian Pharmaceutical Journal 22, no. 3 (2023): 456–65. http://dx.doi.org/10.4103/epj.epj_30_23.

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Background Cellulase is the most employed industrial enzyme in biological conversion of many cellulosic wastes. In this work, economic cellulase production by fungi in solid-state fermentation (SSF) by using solid wastes of medicinal plants was studied. Optimization of growth conditions for production of cellulase was the main target of this study. Objective The current study aimed to isolate and screen fungal isolates that have the ability to produce enzymes to degrade solid wastes of medicinal plant process and optimization of growth factors that affect cellulase production. Materials and me
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Olszak-Przybyś, Hanna, Grażyna Korbecka-Glinka, and Elżbieta Patkowska. "Identification and Pathogenicity of Fusarium Isolated from Soybean in Poland." Pathogens 12, no. 9 (2023): 1162. http://dx.doi.org/10.3390/pathogens12091162.

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Fungi belonging to the Fusarium genus are commonly isolated from soybean plants and seeds but not all of them are pathogenic. The aim of this study was to compare the pathogenicity among different Fusarium isolates obtained from soybean plants with disease symptoms originating from an experimental field located in the southeast of Poland. Nineteen fungal isolates were selected for the pathogenicity assay, including eight isolates of F. oxysporum, six isolates of F. graminearum, four isolates of F. culmorum and one isolate of F. redolens. Species identification of these isolates was carried out
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Krivorutchenko, Yu, M. Kirsanova, O. Postnikova, I. Andronovskaja, and E. Sheiko. "Multidrug resistance to fluconazole, amphotericin B and antiseptic miramistin in Candida spp. isolated from patients with various pathology in region of Crimea." Immunopathology, Allergology, Infectology 2021, no. 3 (2021): 79–85. http://dx.doi.org/10.14427/jipai.2021.3.79.

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To overcome spreading of multidrug resistance to antimycotics in yeast fungi using combination therapy, it is necessary to monitor the sensitivity of clinical fungal isolates to antifungal drugs and antiseptics. The aim of this work was to study multidrug resistance to fluconazole (FLU), amphotericin B (AMB) and antiseptic miramistin (MST) in Candida fungi isolated from patients with various pathologies in Crimea in 2010-2016. Material and methods. The sensitivity of 25 clinical isolates of fungi species C. albicans, C. dubliniensis, C. parapsilosis, C. lusitaniae, C. tropicalis, C. famata, C.
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Nurbailis, Nurbailis, Martinius Martinius, and Verry Azniza. "KEANEKARAGAMAN JAMUR PADA Rizosfer TANAMAN CABAI SISTEM KONVENSIONAL DAN ORGANIK DAN POTENSINYA SEBAGAI AGEN PENGENDALI HAYATI Colletotrichum gloeosporioides." Jurnal Hama dan Penyakit Tumbuhan Tropika 14, no. 1 (2014): 16–24. http://dx.doi.org/10.23960/j.hptt.11416-24.

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ABSTRACTFungal diversity of chili rhizosphere at conventional and organic cropping system and its role as biocontrol agent of Colletotrichum gloeosporioides. The aim of this research was to study fungal diversity in chili rhizosphere at conventional and organic system; to ditermine and identify the fungi that had antagonistic activity against C. gloeosporioides. Diversities of rhizosphere fungi in chili of conventional and organic system were determined by counting the amount of propagule and isolates. Antagonistic activity was examined by dual cultures and slide culture. Identification was de
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Mukaromah, Berliana Dwi, Saiful Bahri, and Wiwin Misnati Nur Indahsari. "Exploration and Identification of Gliocladium sp as Biological Agent from the Rhizosphere of Tomato." Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) 12, no. 1 (2025): 1–12. https://doi.org/10.23960/jbekh.v12i1.390.

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One of the roles of microorganisms is as a biological agent. This study aims to explore and identify the biological agent of the fungus Gliocladium sp. found in the rhizosphere of tomato plants. Exploration was done by taking samples of tomato plant root soil in five locations using a diagonal plot. Rhizosphere microorganisms were isolated using the multilevel dilution and spread on PDA media added with chloramphenicol. Fungal isolates were identified macroscopically and microscopically. The results of exploring fungal biological agents in the rhizosphere soil of tomato plants revealed four ge
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Adhi, Satriyo Restu. "The Antagonistic Ability of Fungal Isolates from Oyster Spent Media Substrate against Fusarium oxysporum in Chili Plants." International Journal of Agriculture and Biology 32, no. 04 (2024): 308–14. https://doi.org/10.17957/ijab/15.2206.

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One of the main diseases of chili plant in Indonesia is Fusarium wilt caused by the fungus Fusarium oxysporum. This study aims to determine whether fungal isolates isolated from oyster spent media substrate (OSMS) have antagonistic ability against F. oxysporum and are not pathogenic in chili plants. The research was conducted by isolating, identifying and characterizing fungal isolates isolated from OSMS, then conducting dual culture tests between OSMS isolates and F. oxysporum in the laboratory, as well as conducting trials in a seedling house to see the effect of inoculation of OSMS fungal i
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Meadow, Richard, John D. Vandenberg, Anthony M. Shelton, and D. Wesley Watson. "Microbial Control of Cabbage Maggot-Preliminary Screenings of Fungal Isolates, 1995." Arthropod Management Tests 21, no. 1 (1996): 413. http://dx.doi.org/10.1093/amt/21.1.413.

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Abstract Adult CM were exposed to dry conidia of isolates of Beauvaria bassiana (Bb), Metarhizium anisopilae (Ma) or Paeciomyces fumosoroseus (Pf). Two B. bassiana isolates were from Cornell University (P89, L90) isolated from Musca domestica (Diptera: Muscidae), one was from Mycoteh Corp., Butte, MT (Myc 726) originally isolated from southern corn rootworm, Diabrotica undecimpuntata (Coleoptera: Chrysomelidae), re-isolated from a grasshopper, Melanoplus sp. (Orthoptera:Acrididae), and then re-isolated from silverleaf whitefly, Bemesia argentiflora (Homoptera: Aleyrodidae). The other isolates
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Kömpe, Yasemin Özdener, Vildan Akin Mutlu, İbrahim Özkoç, Sevim Demiray, and Bozkurt Serhat. "Fungal diversity and ex vitro symbiotic germination of Serapias vomeracea (Orchidaceae)." Acta botanica Croatica 81, no. 1 (2022): 108–16. http://dx.doi.org/10.37427/botcro-2022-008.

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Conservation of orchids can be possible with effective seed germination and seedling growth methods. In this context, ex vitro symbiotic seed germination and seedling growth of orchid seeds may be convenient and advantageous. In this study, both the diversity of the root endophytic fungi in Serapias vomeracea (Burm.f.) Briq. and the ex vitro effects of these fungi on seed germination, seedling development and tuber formation were revealed. The fungi were isolated monthly for two years from S. vomeracea roots and the isolates were identified based on morphological characters and internal transc
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33

Obulesu, Gundala, Hanumanthapp Ar, and Prabakar Reddy E. "PREVALENCE OF FUNGAL IN OPPORTUNISTIC INFECTION HIV TYPE-1 IN SUBURBAN, KADAPA." Asian Journal of Pharmaceutical and Clinical Research 10, no. 6 (2017): 403. http://dx.doi.org/10.22159/ajpcr.2017.v10i6.19404.

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Objective: Isolate fungal element causing in opportunistic infection of human immunodeficiency virus and correlated with CD4 cell counts.Methods: Different samples are used and media: Sabouraud dextrose agar, using mycological methods such as potassium hydroxide, lactophenol cotton blue mount, India ink preparation, and Gram-staining.Results: Both bacterial and fungal isolates were obtained from 8 cases. Pneumococci and Candida spp. were observed in 3 cases followed by Staphylococcus aureus and Aspergillus in 2 cases, Klebsiella pneumoniae and Geotrichum spp., Pneumococci and Penicillium spp.,
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Doumbou, Cyr Lézin, Vladimir Akimov, and Carole Beaulieu. "Selection and Characterization of Microorganisms Utilizing Thaxtomin A, a Phytotoxin Produced byStreptomyces scabies." Applied and Environmental Microbiology 64, no. 11 (1998): 4313–16. http://dx.doi.org/10.1128/aem.64.11.4313-4316.1998.

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ABSTRACT Thaxtomin A is the main phytotoxin produced by Streptomyces scabies, a causal agent of potato scab. Thaxtomin A is a yellow compound composed of 4-nitroindol-3-yl-containing 2,5-dioxopiperazine. A collection of nonpathogenic streptomycetes isolated from potato tubers and microorganisms recovered from a thaxtomin A solution were examined for the ability to grow in the presence of thaxtomin A as a sole carbon or nitrogen source. Three bacterial isolates and two fungal isolates grew in thaxtomin A-containing media. Growth of these organisms resulted in decreases in the optical densities
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Baral, Bikash, Prabina Rana, and Bijaya Laxmi Maharjan. "ANTIMICROBIAL POTENTIALS OF ENDOPHYTIC FUNGI INHABITING RHODODENDRON ANTHOPOGON D. DON." Ecoprint: An International Journal of Ecology 18 (December 20, 2013): 39–44. http://dx.doi.org/10.3126/eco.v18i0.9397.

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Fungal endophytes have been studied from Rhododendron anthopogon D. Don Manaslu Conservation Area in Nepal. The endophytes were isolated from different parts of Rhododendron viz., root, stem and leaf using potato dextrose agar, malt extract agar and water agar. Altogether eighteen fungal endophytes belonging to nine genera were isolated. The endophytic species isolates belong to the genera Stemphylium, Alternaria, Penicillium, Aspergillus, Trichoderma, Papulaspora, Hansfordia, Wardomyces and Geotrichum. Out of 18 fungal isolates, 8 isolates could display antimicrobial activity inhibiting at le
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36

Abdel-Maksoud, Gomaa, Mahmoud Abdel-Nasser, Mahmoud H. Sultan, et al. "Fungal Biodeterioration of a Historical Manuscript Dating Back to the 14th Century: An Insight into Various Fungal Strains and Their Enzymatic Activities." Life 12, no. 11 (2022): 1821. http://dx.doi.org/10.3390/life12111821.

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This study aims to assess the deterioration aspects of a historical manuscript dating back to the 14th century that was deposited in the Library of the Arabic Language Academy, Cairo, Egypt. The study aims at the exploration of the role of various fungal strains that had colonized this deteriorated manuscript in its biodeterioration through their efficacy in the secretion of various hydrolytic enzymes. To evaluate the deterioration, various techniques, including visual inspection, attenuated total reflectance Fourier transform infrared (ATR-FTIR), scanning electron microscopy (SEM), X-Ray diff
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37

Yadav, Poonam, S. R. Singh, and Mala Trivedi. "Characterization of fungi isolated from cattle dung with potential lignocellulolytic activities." Ecology, Environment and Conservation 29 (2023): 144–51. http://dx.doi.org/10.53550/eec.2023.v29i02s.027.

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Agricultural wastes not only serve as sources of environmental pollutions, but also serve as magnificent source of plant nutrients and natural repositories of bio-tools especially fungi of industrial interest for lignocellulolytic enzymes production. However, residues management particularly in rice–wheat system is a tedious phenomenon due to narrow gap between harvesting and sowing of these two crops. Hence, microbial intervention is one of the key options that may use successfully for their management. Thus, cellulolytic fungi were isolated from the cattle dung collected from various locatio
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Fitriana, Fitriana, Ayyub Harly Nurung, and Afdina Putri Adnan. "Production of Antibiotics from Endophyte Fungi Isolates of Bidara Plants (Ziziphus mauritina L.) Isolates Codes IFAZ-6, IFBZ-6, IFDZ-8 With Variations in Carbon Sources." Journal Microbiology Science 4, no. 2 (2024): 241–48. http://dx.doi.org/10.56711/jms.v4i2.1149.

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Research has been carried out on antibiotic production from endophytic fungal isolates coded IFAZ-6, IFBZ-6, and IFDZ-8 from bidara plants (Ziziphus mauritina L.) with variations in carbon sources. This research aims to determine antibiotic production with variations in carbon sources. The first stage was the purification of each selected endophytic fungal isolate. The endophytic fungal isolate was inoculated into the fermentation medium using a variety of carbon sources, namely glucose, fructose, and galactose for 21 days. The antibiotic activity was carried out using the agar diffusion metho
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Khiyami, Mohammad Ahmed, Moawad Ragab Omar, Kamel Ahmed Abd-Elsalam, and Aly Abd El-Hady Aly. "Bacillus-based biological control of cotton seedling disease complex." Journal of Plant Protection Research 54, no. 4 (2014): 340–48. http://dx.doi.org/10.2478/jppr-2014-0051.

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Abstract To formulate an efficient and eco-friendly strategy for the management of cotton seedling disease complex, pot experiments were conducted and the efficiency of eight Bacillus strains against seven fungi involved in the disease were determined. A greenhouse evaluation of the interaction between fungal isolates and Bacillus strains was carried out. The evaluation revealed a very highly significant Bacillus strains x fungal isolates interaction for all the following parameters: preemergence damping-off, postemergence damping-off, survival, plant height, and dry weight. This interaction i
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40

Southcott, K. A., and J. A. Johnson. "Isolation of endophytes from two species of palm, from Bermuda." Canadian Journal of Microbiology 43, no. 8 (1997): 789–92. http://dx.doi.org/10.1139/m97-113.

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This is the first report of endophytes from the indigenous Bermudian palmetto (Sabal bermudana Bailey) and the introduced Chinese palmetto (Livistona chinensis Jacquin), from Bermuda. Fronds were surface sterilized and 8-mm-diameter disks were removed and placed on 2% malt extract agar. Fungal isolates were obtained from 76 of the 375 disks from both species of palm. Idriella (two species) was the most common taxon isolated from both species of palm, making up 22 of the total 76 isolates, while Aspergillus accounted for 17 of the total 76 isolates. An unidentified isolate (BCP95-A), found in t
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41

Abdullah Al-Kubaisy, Abdullah Kamel, and Huriya Hussein AlJuboori. "Evaluation of Inhibition Efficiency of Some Bacteria Isolated from Greenhouse Soils on the Growth of the Pathogenic Fungus Sclerotinia sclerotiorum that Causes White Rot Disease on Vegetables in the Laboratory." Arab Journal for Plant Protection 40, no. 2 (2022): 140–47. http://dx.doi.org/10.22268/ajpp-40.2.140147.

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Al-Kubaisy, A.K.A. and H.H. Al-Juboor. 2022. Evaluation of Inhibition Efficiency of Some Bacteria Isolated from Greenhouse Soils on the Growth of the Pathogenic Fungus Sclerotinia sclerotiorum that Causes White Rot Disease on Vegetables in the Laboratory. Arab Journal of Plant Protection, 40(2): 140-147. https://doi.org/10.22268/AJPP-40.2.140147 This study aimed to isolate beneficial bacteria from the soil of plastic houses planted with eggplant and cucumber at different locations of Baghdad Governorate and characterize them molecularly in addition to determining their antagonistic ability to
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42

I.U., Yahaya, and Sule M.S. "Isolation and Identification of Fungi Associated with Post-Harvest Spoilage of Apple (Pyrus Malus) Traded in Kano Metropolis, Nigeria." International Journal of Research and Innovation in Applied Science IX, no. VIII (2024): 468–77. http://dx.doi.org/10.51584/ijrias.2024.908041.

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Storage of fruits at suboptimal condition provides fungi to grow and some produce mycotoxins, which can be dangerous/harmful for human consumption. This study was conducted to isolate and identify fungi associated with spoilage of apples sourced from Kano markets, Nigeria. A total of nine (9) fresh apple samples were collected randomly from different supermarkets on September of 2023 in Kano, Nigeria. Each apple was placed in a sterile plastic bag at room temperature (25–30°C) for fifteen days or until fungal growth was evident all over the samples. Growth of fungal colonies on Potato Dextrose
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Balakrishnan, ShabinaM, and Kalpana George. "Editorial on Fungal isolates." Journal of The Academy of Clinical Microbiologists 17, no. 1 (2015): 34. http://dx.doi.org/10.4103/0972-1282.158805.

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Midgley, David J., Susan M. Chambers, and John W. G. Cairney. "Spatial distribution of fungal endophyte genotypes in a Woollsia pungens (Ericaceae) root system." Australian Journal of Botany 50, no. 5 (2002): 559. http://dx.doi.org/10.1071/bt02020.

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Fungal endophytes were isolated from hair roots of Woollsia pungens Cav. (Muell.) and mapped according to the root portions from which they were isolated. A total of 119 isolates was obtained and restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer (ITS) region indicated that the isolate assemblage comprised five RFLP types. ITS sequence comparison revealed that RFLP Types I and II had 99.6–99.8% sequence identity with known ericoid mycorrhizal endophytes from Australian epacrids. The remaining three RFLP types were most similar to non-mycorrhizal ascomyc
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El-, Sayed Hussein Ziedan, Mahmoud Saad Moataza, Ahmed Hemida Khames, Abd El Aziz El Naggar Mohamed, Helmy Mostafa Mostafa, and Gad El-Rab El Samman Magdy. "In vitro: stem cutting a simple technique for determination aggressive potential of fungal isolates causing root rot disease of grapevine." GSC Biological and Pharmaceutical Sciences 11, no. 1 (2020): 141–47. https://doi.org/10.5281/zenodo.4277055.

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Pathogenic soil borne fungi causing epidemic root rot disease on grapevine and high loss of plant growth and fruit yield. Fast detection of aggressive fungal isolates is the first tool for introduce selection effective a agent for management disease progress.&nbsp;<em>In vitro</em>&nbsp;two simple techniques based on stem cutting of grapevine i.e., mycelial growth and cell wall degrading enzymes activities were evaluated for screening of aggressive fungal isolates in comparsion with classical pathogenicity test.&nbsp; Fourteen isolates of fungi associated with root rot disease of grapevine pla
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Philip Ganeesh Moorthy R, Praveena VG, and Sowmya AV. "Primary otomycosis: Assessment of risk factors and identification of fungal agents – A tertiary care hospital experience." Asian Journal of Medical Sciences 14, no. 12 (2023): 86–91. http://dx.doi.org/10.3126/ajms.v14i12.58518.

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Background: Primary otomycosis is one of the common conditions seen in general clinical practices. Although non-fatal, inappropriate management of this condition may lead to serious complications. Aims and Objectives: A cross-sectional study was conducted in our tertiary care hospital to determine the fungal agents in primary otomycosis and its associated risk factors. Materials and Methods: A total of 62 consecutive patients of more than 18 years of age with clinical diagnosis of otomycosis were included in this study. Samples were collected and processed and the fungal isolate was identified
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Gunaedi, Tri, and Arsyam Mawardi. "Sequence analysis of 18SrDNA gene from sagoplast degrading fungi." Berkala Penelitian Hayati 26, no. 2 (2021): 72–78. http://dx.doi.org/10.23869/bphjbr.26.2.20214.

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The bioplastic can be made from sago flour and known as sagoplast. It was widely known that for making bioplastic, the addition of acetic acid and glycerol are needed. Products that are air-dried are easy to grow fungi within a few weeks. This makes the basis for researchers to undestand more about the character and identity of the sagoplast degrading fungi. Characterization and identification were carried out by observed morphology and analyzing the 18SrDNA gene sequence of fungal isolates that had grown on the sagoplast. Fungal isolates morphology showed yellowish-orange color with white thr
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Gunaedi, Tri, and Arsyam Mawardi. "Sequence analysis of 18SrDNA gene from sagoplast degrading fungi." Berkala Penelitian Hayati 26, no. 2 (2021): 72–78. http://dx.doi.org/10.23869/bphjbr.26.2.20214.

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The bioplastic can be made from sago flour and known as sagoplast. It was widely known that for making bioplastic, the addition of acetic acid and glycerol are needed. Products that are air-dried are easy to grow fungi within a few weeks. This makes the basis for researchers to undestand more about the character and identity of the sagoplast degrading fungi. Characterization and identification were carried out by observed morphology and analyzing the 18SrDNA gene sequence of fungal isolates that had grown on the sagoplast. Fungal isolates morphology showed yellowish-orange color with white thr
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Gunaedi, Tri, and Arsyam Mawardi. "Sequence analysis of 18SrDNA gene from sagoplast degrading fungi." Berkala Penelitian Hayati 26, no. 2 (2021): 72–78. http://dx.doi.org/10.23869/bphjbr.26.1.20214.

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The bioplastic can be made from sago flour and known as sagoplast. It was widely known that for making bioplastic, the addition of acetic acid and glycerol are needed. Products that are air-dried are easy to grow fungi within a few weeks. This makes the basis for researchers to undestand more about the character and identity of the sagoplast degrading fungi. Characterization and identification were carried out by observed morphology and analyzing the 18SrDNA gene sequence of fungal isolates that had grown on the sagoplast. Fungal isolates morphology showed yellowish-orange color with white thr
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Asif, Siddiqui M., Amir Asad, Mohommad Arif, and Veena Pandey. "SCREENING OF PECTINASE PRODUCING THERMOPHILIC MUCOR SP. ISOLATED FROM DECOMPOSTING FRUITS AND VEGETABLES." Scientific Temper 1, no. 01 (2010): 75–78. http://dx.doi.org/10.58414/scientifictemper.2010.01.1.10.

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A thermophilic fungal strain producing pectinases was isolated afterprimary screening of 12 different isolates on modified Pectin agar medium usingdifferent compost and decomposed matter collected from the vegetable and fruitmarkets of different cities of U.P. Selection of the fungus was done based on theclearance zones and pectinase enzyme production carried out in solid statefermentation. The fungal isolate was initially identified as a Mucor sp.
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