Gotowe bibliografie tematyczne / Fura-2

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Gotowa bibliografia na temat „Fura-2”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 9 czerwca 2025

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Artykuły w czasopismach na temat "Fura-2"

Hurley, T. W., M. P. Ryan, and R. W. Brinck. "Changes of cytosolic Ca2+ interfere with measurements of cytosolic Mg2+ using mag-fura-2." American Journal of Physiology-Cell Physiology 263, no. 2 (August 1, 1992): C300—C307. http://dx.doi.org/10.1152/ajpcell.1992.263.2.c300.

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In rat pancreatic and submandibular gland acini during exposure to carbachol, changes in the fluorescence emission intensity ratio (R) of acini loaded with mag-fura-2 resemble changes in cytosolic Ca2+ concentration (Ca2+i) in acini loaded with fura-2. Furthermore, changes of R depend on the presence of extracellular Ca2+ (Ca2+o) but are much less influenced by changes in extracellular Mg2+ (Mg2+o). To evaluate interference with measurement of cytosolic Mg2+ (Mg2+i) by changes in Ca2+i, we determined the dissociation constant (Kd) and Hill coefficient (NH) of the Ca(2+)-mag-fura-2 and Mg(2+)-m

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Kass, G. E., D. L. Webb, S. C. Chow, J. Llopis, and P. O. Berggren. "Receptor-mediated Mn2+ influx in rat hepatocytes: comparison of cells loaded with Fura-2 ester and cells microinjected with Fura-2 salt." Biochemical Journal 302, no. 1 (August 15, 1994): 5–9. http://dx.doi.org/10.1042/bj3020005.

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In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. The pattern of Fura-2 quenching by Mn2+ only correlated with the movement of Mn2+ across the plasma membrane.

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Scaduto, Russell C., and Lee W. Grotyohann. "Hydrolysis of Ca2+-sensitive fluorescent probes by perfused rat heart." American Journal of Physiology-Heart and Circulatory Physiology 285, no. 5 (November 2003): H2118—H2124. http://dx.doi.org/10.1152/ajpheart.00881.2001.

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Rat hearts were loaded with the fluorescent calcium indicators fura 2, indo 1, rhod 2, or fluo 3 to determine cytosolic calcium levels in the perfused rat heart. With fura 2, however, basal tissue fluorescence increased above anticipated levels, suggesting accumulation of intermediates of fura 2-AM deesterification. To examine this process, we separated the intermediates of the deesterification process using HPLC after incubation of fura 2-AM with tissue homogenates and after loading in the rat heart. Loading of hearts with fura 2-AM resulted in tissue levels of fura 2 free acid that were only

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Carroll, S. L., M. G. Klein, and M. F. Schneider. "Calcium transients in intact rat skeletal muscle fibers in agarose gel." American Journal of Physiology-Cell Physiology 269, no. 1 (July 1, 1995): C28—C34. http://dx.doi.org/10.1152/ajpcell.1995.269.1.c28.

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Intact single fibers enzymatically dissociated from rat flexor digitorum brevis muscle were suspended in 0.5% low-melting-temperature agarose gel to minimize fiber movement during action potentials or trains of action potentials. Resting Ca2+ concentration ([Ca2+]) and changes in [Ca2+] were monitored using the fluorescent calcium indicator fura 2. The time course and waveform of [Ca2+] transients during an action potential or trains of action potentials in fibers in agarose were calculated using kinetic parameters previously determined to correct for the calcium-fura 2 kinetic delay. Half tim

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Tran, N. N. P., P. Leroy, L. Bellucci, A. Robert, A. Nicolas, J. Atkinson, and C. Capdeville-Atkinson. "Intracellular concentrations of Fura-2 and Fura-2/AM in vascular smooth muscle cells following perfusion loading of Fura-2/AM." Pharmacological Research 31 (January 1995): 206. http://dx.doi.org/10.1016/1043-6618(95)87082-2.

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Becker, P. L., and F. S. Fay. "Photobleaching of fura-2 and its effect on determination of calcium concentrations." American Journal of Physiology-Cell Physiology 253, no. 4 (October 1, 1987): C613—C618. http://dx.doi.org/10.1152/ajpcell.1987.253.4.c613.

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This study was performed to determine the effect of photobleaching on the spectral properties of the calcium-sensitive fluorescent dye fura-2. Fura-2, whether in cells or in calibrating solutions, was found to be bleached when exposed to excitation light. In contrast to the widely held belief, photobleaching altered the spectral properties of the dye. Decomposition of the excitation spectra of partially bleached fura-2 solutions revealed an intermediate that is still fluorescent and is not sensitive to calcium over the same range as fura-2, but which can bind calcium in the millimolar range. T

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Pape, P. C., D. S. Jong, W. K. Chandler, and S. M. Baylor. "Effect of fura-2 on action potential-stimulated calcium release in cut twitch fibers from frog muscle." Journal of General Physiology 102, no. 2 (August 1, 1993): 295–332. http://dx.doi.org/10.1085/jgp.102.2.295.

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Cut fibers (striation spacing, 3.6-4.2 microns) were mounted in a double Vaseline-gap chamber and studied at 14-15 degrees C. One or both of the Ca indicators fura-2 and purpurate-3,3' diacetic acid (PDAA) were introduced into the optical recording site by diffusion from the end pools. Sarcoplasmic reticulum (SR) Ca release was elicited by action potential stimulation. With resting [fura-2] = 0 mM at the optical site, the [Ca] transient measured with PDAA was used to estimate SR Ca release (Baylor, S.M., W.K. Chandler, and M.W. Marshall. 1983. Journal of Physiology. 344:625-666). With resting

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Jensen, P. E., M. J. Mulvany, C. Aalkjaer, H. Nilsson, and H. Yamaguchi. "Free cytosolic Ca2+ measured with Ca(2+)-selective electrodes and fura 2 in rat mesenteric resistance arteries." American Journal of Physiology-Heart and Circulatory Physiology 265, no. 2 (August 1, 1993): H741—H746. http://dx.doi.org/10.1152/ajpheart.1993.265.2.h741.

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Free cytosolic Ca2+ was measured with sub-micrometer-tip, double-barrelled, Ca(2+)-selective electrodes and fura 2 in rat mesenteric resistance arteries. The purpose was to establish intracellular free Ca2+ concentration ([Ca2+]i) values in resting and stimulated vessels. Isolated vessels were mounted for isometric force measurements. Measured with electrodes, mean [Ca2+]i was 115 and 708 nM under resting and norepinephrine-activated conditions, respectively. Fura 2 was calibrated intracellularly including determination of the intracellular dissociation constant (Kd) of the fura 2:Ca2+ complex

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Steinberg, S. F., J. P. Bilezikian, and Q. Al-Awqati. "Fura-2 fluorescence is localized to mitochondria in endothelial cells." American Journal of Physiology-Cell Physiology 253, no. 5 (November 1, 1987): C744—C747. http://dx.doi.org/10.1152/ajpcell.1987.253.5.c744.

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The new, highly fluorescent, calcium-sensitive dye, fura-2, can be loaded nondisruptively into intact cells by means of its permeant ester and used to measure the free calcium ion concentration in individual cells. For fura-2 to signal cytosolic calcium, it must be distributed homogeneously and exclusively throughout the cytoplasmic space. However, microscopic examination of bovine aortic endothelial cells loaded with fura-2 by exposure to its permeant ester reveals fluorescence associated with discrete intracellular structures rather than the homogeneous distribution expected for a cytosolic

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Di Virgilio, F., T. H. Steinberg, J. A. Swanson, and S. C. Silverstein. "Fura-2 secretion and sequestration in macrophages. A blocker of organic anion transport reveals that these processes occur via a membrane transport system for organic anions." Journal of Immunology 140, no. 3 (February 1, 1988): 915–20. http://dx.doi.org/10.4049/jimmunol.140.3.915.

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Abstract Fura-2, loaded into J774.2 macrophages as the acetoxymethyl ester, is sequestered into intracellular vacuoles within 90 min after the beginning of the loading at 37 degrees C. The dye is also efficiently secreted from the cells. Sequestration and secretion of fura-2 reduce the accuracy of measurements of cytosolic free Ca2+ concentration in this cell line. Fura-2 is also sequestered and secreted by J774.2 when the dye is loaded into the cytoplasm as the pentapotassium salt by reversible permeabilization of the plasma membrane. Regardless of the mechanism by which fura-2 is loaded into

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Rozprawy doktorskie na temat "Fura-2"

Silva, Orivaldo Lopes da. "Incorporação de cálcio iônico em células ósseas induzida por campo elétrico." Universidade de São Paulo, 1995. http://www.teses.usp.br/teses/disponiveis/76/76132/tde-10062014-163725/.

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Acredita-se que sinais elétricos endógenos afetem remodelamento, metabolismo, reparo e crescimento ósseos. Existe uma ampla literatura que trata do efeito de sinais elétricos externos sobre as respostas de síntese, mitogênese e proliferação em osteoblastos e células ósseas in vitro. Acredita-se que as respostas fisiológicas ao estimulo elétrico sejam devidas a mecanismos celulares que envolvem variações na concentração citosólica de cálcio. No presente estudo esse efeito celular foi observado através da estimulação direta, por campo elétrico de intensidade fisiologicamente significativa de 10m

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Hadrovic, Banina. "A study of TRPV1 and TRPV4 ion channels in the beta cells by using fura-2 based microfluorometry." Thesis, Mälardalen University, School of Sustainable Development of Society and Technology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-7350.

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The calcium ion (Ca2+) is an important ion that regulates many cellular functions including exocytosis, contraction of muscles, neural functions, fertilization and cell division. In the plasma membrane of cells there are different Ca2+ channels, including the transient receptor potential (TRP) family of cation channels. The TRP channels are activated by physical stimuli like temperature, stretch, osmolality, and also various ligands. These channels are divided into seven subfamilies, namely TRPC, TRPV, TRPM, TRPML, TRPA, TRPP, and TRPN. TRP channels can r

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Beurg, Maryline. "Couplage excitation-contraction des cellules musculaires striées : étude des variations transitoires de calcium par imagerie de fura-2." Bordeaux 1, 1995. http://www.theses.fr/1995BOR10530.

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Le couplage excitation-variation transitoire de ca2+ (e-vt) constitue l'etape cle du couplage e-c des muscles squelettiques. A l'aide de la technique d'imagerie du fura-2, nous avons suivi la dynamique et la distribution spatiale de la concentration de calcium intracellulaire pendant le developpement des myotubes de rat, de souris dysgeniques et humains en culture. Les myotubes de rat in vitro presentent temporairement un stade dit quiescent durant lequel ces cellules sont incapables de se contracter alors qu'elles possedent un couplage e-vt de ca2+ fonctionnel. Lors du couplage e-vt, la liber

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Lascombe, Marie-Laure. "Le couplage excitation-contraction dans les cellules musculaires striées normales et dysgéniques : étude par imagerie de fluorescence de fura-2." Bordeaux 1, 1992. http://www.theses.fr/1992BOR10519.

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Pour etudier les mecanismes du couplage excitation-contraction dans les cellules musculaires striees normales et dysgeniques (mdg/mdg) nous avons suivi la dynamique et la distributionspatiale des variations de la ca#2#+#i par videomicroscopie de fluorescence et traitement des images. Dans les myotubes normaux, nous avons trouve: a) l'existence, dans une etape de la myogenese, d'un couplage excitation-augmentation de la ca#2#+#i n'induisant pas de contraction; b) sous l'action de l'acetylcholine, la variation calcique est biphasique avec d'abord la liberation de calcium apparemment module par u

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Castro, Kraftchenko Joel, and kraf0005@flinders edu au. "STORE OPERATED Ca2+ CHANNELS IN LIVER CELLS: REGULATION BY BILE ACIDS AND A SUB-REGION OF THE ENDOPLASMIC RETICULUM." Flinders University. Medicine, 2008. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20080826.135311.

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Cholestasis is an important liver pathology. During cholestasis bile acids accumulate in the bile canaliculus affecting hepatocyte viability. The actions of bile acids require changes in the release of Ca2+ from intracellular stores and in Ca2+ entry. The target(s) of the Ca2+ entry pathway affected by bile acids is, however, not known. The overall objective of the work described in this thesis was to elucidate the target(s) and mechanism(s) of bile acids-induced modulation of hepatocytes calcium homeostasis. First, it was shown that a 12 h pre-incubation with cholestatic bile acids (to mimic

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Scanlon, Mary. "Cellular mechanism of neutrophil chemotaxis: the role of CA⁺², as viewed with the fluorescent dye, FURA-2, in the polarization of human polymorphonuclear leukocytes following stimulation with the chemoattractant, F-Methionyl-Leucyl-Phenylalanine: a thesis." eScholarship@UMMS, 1987. https://escholarship.umassmed.edu/gsbs_diss/320.

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The mechanism by which a cell translates a spatially oriented, extracellular signal into a change in morphology and behavior is the key to understanding many biological processes. In order to investigate this general phenomenon, I have studied the chemotactic response of human polymorphonuclear leukocytes (PMN's) to f-methionyl-leucyl-phenylalanine (fMLP). Stimulation of PMN's with fMLP produces a plethora of intracellular events, including increases in cytosolic Ca+2. PMN's are also morphologically and behaviorally polarized by stimulation with chemoattractant; the membrane components and cyt

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Westerlund, Johanna. "Pulsatile insulin release from single islets of Langerhans." Doctoral thesis, Uppsala University, Department of Medical Cell Biology, 2000. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-494.

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Insulin release from single islets of Langerhans is pulsatile. The secretory activities of the islets in the pancreas are coordinated resulting in plasma insulin oscillations. Nutrients amplitude-regulate the insulin pulses without influencing their frequency. Diabetic patients show an abnormal plasma insulin pattern, but the cause of the disturbance remains to be elucidated. Ithe present thesis the influence of the cytoplasmic calcium concentratio([Ca2+]i) and cell metabolism on pulsatile insulin release was examined in single islets of Langerhans from ob/ob-mi

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Egunlusi, Ayodeji Olatunde. "Novel tricycloundecane derivatives as potential N-methyl-Daspartate receptor and calcium channel inhibitors for neuroprotection." Thesis, University of the Western Cape, 2014. http://hdl.handle.net/11394/3904.

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>Magister Scientiae - MSc This study focused on the synthesis of a series of novel tricycloundecane derivatives and evaluation of these compounds for neuroprotection using the fluorescent ratiometric calcium assay that indicates the ability of the test compounds to inhibit NMDA receptors and VGCC. The cycloaddition reaction between p-benzoquinone and monomerised dicyclopentadiene yielded tricycloundeca- 4,9-diene-3,6-dione which was used as the base structure and further derivatised. These derivatives were conjugated with benzylamine to form a series of imines and amines. A total of 10 comp

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Liu, Li. "Roles of PMCA Isoforms in Ca²⁺-Homeostasis and Contractility of Bladder Smooth Muscle: Evidence from PMCA Gene-Ablated Mice." Cincinnati, Ohio : University of Cincinnati, 2007. http://rave.ohiolink.edu/etdc/view.cgi?acc_num=ucin1178307168.

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Thesis (Ph.D.)--University of Cincinnati, 2007. Advisor: Richard J. Paul. Title from electronic thesis title page (viewed Apr. 4, 2009). Keywords: PMCA (human gene symbols; ATP2B); SERCA2 (human gene symbols; ATP2A2); NCX; bladder smooth muscle; Ca²⁺ homeostasis; gene-altered mice. Ca²⁺ waves; Ca²⁺ sparks; Fura-PE3; Fluo-4; Indo-1; multi-photon microscopy. Includes abstract. Includes bibliographical references.

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Ahmed, Meftun. "Oscillatory Ca²⁺ signaling in glucose-stimulated murine pancreatic β-cells : Modulation by amino acids, glucagon, caffeine and ryanodine". Doctoral thesis, Uppsala University, Department of Medical Cell Biology, 2001. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-1408.

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Oscillations in cytoplasmic Ca2+ concentration ([Ca2+]i) is the key signal in glucose-stimulated β-cells governing pulsatile insulin release. The glucose response of mouse β-cells is often manifested as slow oscillations and rapid transients of [Ca2+] i. In the present study, microfluorometric technique was used to evaluate the role of amino acids, glucagon, ryanodine and caffeine on the generation and maintenance of [Ca2+] i oscillations and transients in individual murine β-cells and isolated mouse pancreatic isl

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Książki na temat "Fura-2"

Kogelnik, Hans-Joachim. Neue Methoden zur Darstellung von (5H)-Furan-2-Onen und (2H)-Pyran-2-Onen. [s.l.]: [s.n.], 1985.

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Perry, Philip J. Synthesis and biological evaluation of novel cytotoxic heterocyclic compounds: Furo (2,3-b) naphthoquinones and 2-aryl-4H-3,1-benzoxazin-4-ones. Leicester: De Montfort University, 1996.

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Moore, URS Dames &. Inventory of dioxin and furan emissions to air, land and water in Ireland for 2000 and 2010 (2000-DS-2-M1): Synthesis report. Johnstown Castle, Co. Wexford: Environmental Protection Agency, 2002.

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Markaz Dirāsāt al-Khalīj wa-al-Jazīrah al-ʻArabīyah, ред. Nadwat Duwal Majlis al-Taʻāwun al-Khalījī wa-Juhūd Taḥqīq al-Amn wa-al-Istiqrār khilāla al-ʻAqd al-Qādim, al-Furaṣ wa-al-Quyūd: 1-2 Māyū 2001, al-Kuwayt. [Kuwait]: Markaz Dirāsāt al-Khalīj wa-al-Jazīrah al-ʻArabīyah, Jāmiʻat al-Kuwayt, 2001.

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Suzuki, Hidetaku. Jugyō-zukuri o chūshin ni kamaeta kyōdō kenkyū no soshikika: Kyōshoku daigakuin de mananda koto o furu ni ikashi, kenkyū kadai "manabiyasui kankyō no sōzō, yorokobi ga kanjirareru jugyō o mezashite" no moto kenkyū o susumeta, kenkyū shunin to shite no 2-nenkan o furikaette. Fukui-shi: Fukui Daigaku Daigakuin Kyōikugaku Kenkyūka Kyōshoku Kaihatsu Senkō, 2009.

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Arnold, J. Douglas, and Zach Meston. Awesome Sega Genesis Secrets 3. Lahaina, HI: Sandwich Islands Publishing, 1993.

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Prima. Official Sega Genesis: Power Tips Book, Volume 3. Rocklin, CA: Prima Publishing, 1994.

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Stilton, Gerónimo. Ci tengo alla pelliccia, io! Milino: Piemme, 2000.

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Breisgau, Universität Freiburg im, ed. Störungen der Ca-2+-Homöostase am insuffizienten menschlichen Myokard: Vergleich der beiden Ca2+-Indikatoren Fura-2 und Aequorin ; pharmakologische Beeinflussung des sarkoplasmatischen Retikulums. 1998.

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Fury Book #2/blood Ra (Fury Book, No. 2). Berkley, 1992.

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Części książek na temat "Fura-2"

Grouselle, M., and D. Georgescauld. "Fura-2 Imaging of Intracellular Free Calcium Dynamics in Excitable Cells." In Water and Ions in Biomolecular Systems, 229–40. Basel: Birkhäuser Basel, 1990. http://dx.doi.org/10.1007/978-3-0348-7253-9_23.

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Patel, Anish, Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert. "Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2." In Methods in Molecular Biology, 37–47. Totowa, NJ: Humana Press, 2012. http://dx.doi.org/10.1007/978-1-62703-086-1_2.

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Xu, Yan-Jun, Qiming Shao, and Naranjan S. Dhalla. "Fura-2 fluorescent technique for the assessment of Ca2+ homeostasis in cardiomyocytes." In Novel Methods in Molecular and Cellular Biochemistry of Muscle, 149–57. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-6353-2_16.

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Smith, Stephen J., Luis R. Osses, and George J. Augustine. "Fura-2 Imaging of Localized Calcium Accumulation Within Squid ‘Giant’ Presynaptic Terminal." In Calcium and Ion Channel Modulation, 147–55. Boston, MA: Springer US, 1988. http://dx.doi.org/10.1007/978-1-4613-0975-8_12.

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Johnson, Martin. "Calcium Imaging of Store-Operated Calcium (Ca2+) Entry (SOCE) in HEK293 Cells Using Fura-2." In Calcium Signalling, 163–72. New York, NY: Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9018-4_15.

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Wiltink, Anneke, Arnoud van der Laarse, Nel P. M. Herrmann-Erlee, Joke M. van der Meer, and Dirk L. Ypey. "The Use of the Fluorescent Probe Fura-2 For Intracellular Free Calcium Measurements: Some Methodological Aspects." In Biotechnology Applications of Microinjection, Microscopic Imaging, and Fluorescence, 133–42. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2828-9_16.

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Hayes, Brendan A., P. V. Avdonin, and U. S. Ryan. "MAG-FURA-2 Elicited Fluorescent Response of Subcellular Magnesium in Endothelial Cells: A Sharp Contrast with Calcium." In Vascular Endothelium, 256–57. Boston, MA: Springer US, 1991. http://dx.doi.org/10.1007/978-1-4615-3736-6_35.

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De Nadai, Andrea, Nicola Vajente, Diana Pendin, and Andrea Mattarei. "Mt-fura-2, a Ratiometric Mitochondria-Targeted Ca2+ Sensor. Determination of Spectroscopic Properties and Ca2+ Imaging Assays." In Methods in Molecular Biology, 187–215. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1262-0_12.

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Hayashi, H., N. Noda, H. Miyata, S. Suzuki, A. Kobayashi, M. Hirano, T. Kawai, T. Hayashi, and N. Yamazaki. "Changes in Cell Morphology, [Ca2+]i and pHi During Metabolic Inhibition in Isolated Myocytes of Diabetic Rats Using Dual-Loading of Fura-2 and BCECF." In Developments in Cardiovascular Medicine, 183–98. Boston, MA: Springer US, 1992. http://dx.doi.org/10.1007/978-1-4615-3512-6_18.

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Winkelmann, J. "Diffusion of air (1); 2-methyl-furan (2)." In Gases in Gases, Liquids and their Mixtures, 1816. Berlin, Heidelberg: Springer Berlin Heidelberg, 2007. http://dx.doi.org/10.1007/978-3-540-49718-9_1374.

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Streszczenia konferencji na temat "Fura-2"

Pribulova, Alena, Peter Futas, Patrik Fedorko, Maria Mihalikova, and Maria Mihalikova. "THE EFFECT OF THE AMOUNT OF BINDER ON THE PROPERTIES OF BRIQUETTES PREPARED FROM FOUNDRY DUST." In 24th SGEM International Multidisciplinary Scientific GeoConference 24, 307–14. STEF92 Technology, 2024. https://doi.org/10.5593/sgem2024/4.1/s18.41.

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Briquetting is the pressing of dusty materials into pieces of the same shape and size. Briquetting can be done without a binder or with the addition of a binder to the briquetting mixture. Briquettes can have any shape, but the most common is the shape of a cylinder. The briquettes must be strong enough to eliminate the possibility of damage during transport and must be strong enough even at high temperatures. The briquettes have a regular shape, have the same weight, are more durable and easier to transport. They are ecologically safe and it is possible to use carbon filling in briquettes to

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Poll, C. T., and J. Westwick. "THE ROLE OF IONISED INTRACELLULAR FREE CALCIUM ([Ca++]i) IN THROMBIN-INDUCED DENSE GRANULE SECRETION." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644475.

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Fura 2 is one of a recently-introduced family of Ca++ indicators with improved fluorescent properties compared to quin 2 (Grynkiewicz et al 1985). This study has examined the role of [Ca++]i in thrombin-induced dense granule release using prostacyclin-washed human platelets loaded with either thedense granule marker 14C-5HT (5HT) alone or with 5HT together with quin 2 ([quin2]i = 0.8mM) or fura 2 ([fura 2]i 20-30µM). In the presence of ImM extracellular calcium concentration ([Ca++]i) the [Ca++]e in quin 2 and fura 2 loaded platelets was 93±2 (n=10 experiments) and 133±0.3nM (n=12 experiments)

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Koyama, M., F. Katabami, K. Matsuno, H. Matsumiya, K. Abe, K. Sakurada, S. Ozasa, I. Maekawa, and T. Miyazaki. "THROMBIN-INDUCED BIPHASIC Ca2+TRANSIENT DETECTED BY FURA-2 FLUORESCENCE WAS COUPLED WITH BIPHASIC Ca2+ UPTAKE IN PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644476.

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In the last congress on Thrombosis and Haemostasis in SanDiego we presented the biphasic Ca2+transient in thrombin-stimulated platelets detected by quin2. This time we tried to measure Ca2+ transient in platelets using other Ca2+indicators such as fura-2 and aequorin. In order to evaluate the contribution of increased Ca2+ influx across the plasma-membrane in elevating cytosolic free Ca concentration on platelet activation, 45 Ca2+uptake was measured simultaneouslyby silicon-oil centrifugation method with or without 5 mM EGTA treatment. Such EGTA treatment was intended to remove the entire 45C

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Lanza, F., A. Beretz, M. Kubina, and J.-P. Cazenave. "INCREASED AGGREGATION AND SECRETION RESPONSES OF HUMAN PLATELETS WHEN LOADED WITH THE CALCIUM FLUORESCENT PROBES QUIN2 AND FURA-2." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643760.

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Incubation of human platelets with the fluorescent dye esters quin2-AM (10 μM) or fura-2-AM (1 μM) makes possible the direct measurement of intracellular free calcium ([Ca2+1).Underthese conditions, basal levels of [Ca2+]i of 120 ± 16 nM (n=23) using quin2 and 137 ± 15 nM (n=5) using fura-2 can be measured. Both probes record comparable increases of [Ca2 ]i after stimulation with ADP, thrombin, PAF, or U-46619. Incorporation into human platelets of quin2 or fura-2 at the concentrations used to monitor [Ca2+]i leads to the activation of platelets. This was shown by increased aggregation and sec

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Van den Bergh, Viviane, Katrien Meuwis, Noel Boens, Frans C. De Schryver, Michel Vincent, Jacques Gallay, and Marcel Ameloot. "Photophysical study of the Ca²⁺ indicator Fura-2 and the K⁺ indicator PBFI." In OE/LASE '94, edited by Joseph R. Lakowicz. SPIE, 1994. http://dx.doi.org/10.1117/12.182705.

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Rao, Gundu H. R., and James G. White. "INFLUENCE OF CALCIUM FLUX ON STABILITY OF PLATELET MICROTUBULE COILS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643904.

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Resting human platelets have a characteristic discoid form supported by a circumferential microtubule (MT). Ultrastructural, immunocytochemical and immunofluorescence studies have shown that the circumferential MT is a stable structure which undergoes constriction following exposure of platelets to aggregating agents. However, some biochemical and morphological studies suggested that MT coils dissolved almost completely within seconds after exposure to aggregating agents, then reassembled 1-4 minutes later. One investigation suggested that disappearance of the MT coils was associated with calc

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Watts, I. S., R. J. Keery, and P. Lumlev. "EFFECT OF EXTRACELLULAR Ca2+ UPON AGONIST-INDUCED Ca2+ TRANSIENTS IN HUMAN PLATELETS: COMPARISON OF QUIN 2 AND FURA 2." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644525.

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In human platelets loaded with the Ca2+ indicator Quin 2 (Q2), elevated cytosolic Ca2+ ([Ca2+]i) induced by platelet agonists is greatly attenuated in the absence of extracellular Ca2+ ([Ca2+]o), suggesting the majority of [Ca2+]i is derived via transmembrane influx. However, the relatively weak fluorescent properties of Q2 require that high intraplatelet concentrations be used which may influence [Ca2+]i homeostasis and platelet function. This is less of a problem with the more intensely fluorescent Ca2+-indicator Fura 2 (F2). We have compared the rise in [Ca2+]i and aggregation induced by a

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Aswani, Kavita. "LED illumination for fluorescence from Fura-2 to Cy7.5 and beyond: A true lamp replacement." In Light-Emitting Devices, Materials, and Applications XXV, edited by Martin Strassburg, Jong Kyu Kim, and Michael R. Krames. SPIE, 2021. http://dx.doi.org/10.1117/12.2576651.

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Matsuno, K., F. Katabami, M. Koyama, K. Abe, K. Sakurada, T. Miyazaki, S. Ozasa, H. Saitoh, I. Maekawa, and H. Matsumiya. "PLATELET-ACTIVATING FACTOR (PAF)-INDUCED INTRACELLULAR Ca MOBILIZATION IN HUMAN PLATELETS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643483.

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PAF-induced intracellular Ca2+ mobilization and platelet aggregation were investigated in human platelets. Cytosolic free Ca2+ concentration ([Ca2+]cyt) was measured by using fluorescent 45Ca2+ probe quin2 and fura-2, and photoprotein aequorin. Ca2+ uptake was measured after stimulation by PAF. Platelet aggregation was studied by recording the change in light transmission with platelet rich plasma (PRP) or washed platelet suspension (WPS).These three Ca2+ -indicators could determine the elevation of [Ca2+ ]cyt that was stimulated by PAF in the presence of extra- cellular Ca2+ (quin2 method: 98

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Lascombe, M. L., M. Grouselle, J. P. Desmazès, D. Georgescauld, J. Koenig, and J. Chapron. "FURA-2 imaging of intracellular free Ca2+ dynamics and distribution induced by acetylcholine and caffeine in embryonic skeletal muscle cells." In The living cell in four dimensions. AIP, 1991. http://dx.doi.org/10.1063/1.40602.

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Raporty organizacyjne na temat "Fura-2"

Philosoph-Hadas, Sonia, Richard Crain, Shimon Meir, Nehemia Aharoni, and Susan Lurie. Calcium-Mediated Signal Transduction during Leaf Senescence. United States Department of Agriculture, November 1995. http://dx.doi.org/10.32747/1995.7604925.bard.

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We have examined the possibility that modulation of [Ca2+]cyt may represent a signal which induces senescence processes in leaves, through triggering of lipid hydrolysis leading to the cascade of detriorative events. Characterization of the signal transduction components operating during leaf senescence was gained by studying various Ca2+-dependent activities of parsley and chrysanthemum leaves, in relation to several senescence functions, and in response to senescence-modulating hormones (ethylene,ABA, BA and IAA). Some innovative findings regarding the control of senescence processes by [Ca2

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Rouseff, Russell L., and Michael Naim. Characterization of Unidentified Potent Flavor Changes during Processing and Storage of Orange and Grapefruit Juices. United States Department of Agriculture, September 2002. http://dx.doi.org/10.32747/2002.7585191.bard.

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Citrus juice flavor quality traditionally diminishes after thermal processing and continuously during storage. Our prior studies found that four of the five most potent off-aromas formed during orange juice storage had not been identified. The primary emphasis of this project was to characterize and identify those potent flavor degrading aroma volatiles so that methods to control them could be developed and final flavor quality improved. Our original objectives included: 1 Isolate and characterize the most important unidentified aroma impact compounds formed or lost during pasteurization and s

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Jocelyn, Sabrina, Élise Ledoux, Damien Burlet-Vienney, Isabelle Berger, Isvieysys Armas Marrero, Chun Hong Law, Yuvin Chinniah, et al. Identification en laboratoire des éléments essentiels au processus d’intégration sécuritaire de cellules cobotiques. IRSST, August 2024. http://dx.doi.org/10.70010/qkwy4060.

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Les cobots sont apparus vers 2010 en industrie et les accidents sont très peu documentés. La gestion des risques en cobotique représente un réel défi. La littérature scientifique montre l’existence de divers modèles, méthodes et outils pour gérer les risques en cobotique, en mettant l’opérateur humain au cœur de l’intégration des applications collaboratives. Cependant, un autre humain clé de la mise en œuvre de ces applications est négligé la plupart du temps. Il s’agit de l’intégrateur, celui qui doit concevoir la cellule cobotique. À notre connaissance, deux études portant sur un même projet

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