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Rozprawy doktorskie na temat „Genetic transformation”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Zainuddin. "Genetic transformation of wheat (Triticum aestivum L.)." Title page, Contents and Abstract only, 2000. http://web4.library.adelaide.edu.au/theses/09APSP/09apspz21.pdf.

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Bibliography: leaves 127-151. The successful application of genetic engineering in wheat is dependent on the availability of suitable tissue culture and transformation methods. The primary object of this project was the development of these technologies using elite Australian wheat varieties.

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2

Button, Eric A. "Regulation of T-DNA gene 7." Thesis, University of British Columbia, 1987. http://hdl.handle.net/2429/26177.

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The purpose of this study was two-fold. The first objective was to determine if Saccharomyces cerevisiae is a useful system for investigating the expression of T-DNA (it takes several months to obtain sufficient bacteria-free transformed plant tissue to investigate T-DNA transcription). A short fragment of T-DNA carrying T-DNA gene 7 was cloned into a yeast plasmid in an attempt to investigate the expression of gene 7 in yeast. The second objective was to determine the significance of a heat shock related sequence identified in the 5¹ region of T-DNA gene 7. Primer extension analysis, SI nucl

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3

Tor, Mahmut. "Genetic transformation of yam (Dioscorea)." Thesis, Imperial College London, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267504.

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4

Gartland, Kevan M. A. "Studies on plant genetic transformation." Thesis, University of Nottingham, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236507.

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Fryer, Shirley Anne. "Genetic transformation of oilseed rape." Thesis, University of Wolverhampton, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317928.

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6

Chen, Dong Fang. "Genetic transformation in the Gramineae." Thesis, Open University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293321.

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7

Soloki, Mahmod. "Genetic transformation of grape somatic embryos." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.387659.

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8

Faria, Maria José Sparça Salles de. "Red raspberry transformation using agrobacterium." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=69522.

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Regeneration and transformation protocols for 'Comet' red raspberry were optimized with the purpose of making the Agrobacterium-mediated gene transfer system efficient for this crop. Adventitious shoot regeneration from leaf discs was improved using explants 10 mm in diameter and transferring to fresh medium at the fourth week of incubation. Additions of liquid medium to solid medium during incubation decreased regeneration and attempts to release the suppressive influence of larger shoots on initials (apical dominance) did not succeed. The presence of claforan did not affect shoot regeneratio

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9

Robson, Julia. "The construction of an expression vector for the transformation of the grape chloroplast genome." Thesis, Stellenbosch : Stellenbosch University, 2003. http://hdl.handle.net/10019.1/53621.

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Thesis (MSc)--University of Stellenbosch, 2003.<br>ENGLISH ABSTRACT: The genetic information of plants is found in the nucleus, the mitochondria, and the plastids. The DNA of plastids is comprised of multiple copies of a double-stranded, circular, prokaryoticallyderived genome of -150 kb. The genome equivalents of plastid organelles in higher plant cells are an attractive target for genetic engineering as high protein expression levels are readily obtained due to the high genome copy number per organelle. The resultant proteins are contained within the plastid organelle and the correspon

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10

Cook, Marisa Anne. "Replicons derived from endogenously isolated plasmids used to classify plasmids occurring in marine sediment bacteria." Thesis, Georgia Institute of Technology, 2001. http://hdl.handle.net/1853/25736.

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11

Stummer, Belinda E. "Micropropagation and genetic transformation of 'verticordia grandis' /." Adelaide : Thesis (Ph.D.)--University of Adelaide, Departments of Plant and and Soil Sciences, 1993. http://web4.library.adelaide.edu.au/theses/09PH/09phs934.pdf.

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12

Singh, S. "Somatic embryogenesis and genetic transformation in peanut." Thesis(Ph.D.), CSIR-National Chemical Laboratory, Pune, 2008. http://dspace.ncl.res.in:8080/xmlui/handle/20.500.12252/2710.

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13

Wu, Jiang. "Transformation of Rhizoctonia solani." Thesis, Wu, Jiang (2003) Transformation of Rhizoctonia solani. PhD thesis, Murdoch University, 2003. https://researchrepository.murdoch.edu.au/id/eprint/417/.

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The aim of this study was to develop a genetic transformation system for the plant pathogenic fungus Rhizoctonia solani (teliomorph, Thanatephprus cucumeris Frank [Donk]). The availability of a transformation system would allow us to study gene exchange, epidemiology, and to use techniques such as gene disruption or gene silencing to investigate the role of fungal enzymes in pathogenesis. The approach adopted was to use Agrobacterium tumefaciens to transform the fungus as reports in the literature suggested that this was the most efficient and easiest method to use. As a preliminary test,

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14

Wu, Jiang. "Transformation of Rhizoctonia solani." Wu, Jiang (2003) Transformation of Rhizoctonia solani. PhD thesis, Murdoch University, 2003. http://researchrepository.murdoch.edu.au/417/.

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The aim of this study was to develop a genetic transformation system for the plant pathogenic fungus Rhizoctonia solani (teliomorph, Thanatephprus cucumeris Frank [Donk]). The availability of a transformation system would allow us to study gene exchange, epidemiology, and to use techniques such as gene disruption or gene silencing to investigate the role of fungal enzymes in pathogenesis. The approach adopted was to use Agrobacterium tumefaciens to transform the fungus as reports in the literature suggested that this was the most efficient and easiest method to use. As a preliminary test,

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15

Williamson, Phillip C. "A Novel Mechanism for Site-Directed Mutagenesis of Large Catabolic Plasmids Using Natural Transformation." Thesis, University of North Texas, 2001. https://digital.library.unt.edu/ark:/67531/metadc2828/.

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Natural transformation is the process by which cells take up DNA from the surrounding medium under physiological conditions, altering the genotype in a heritable fashion. This occurs without chemical or physical treatment of the cells. Certain Acinetobacter strains exhibit a strong tendency to incorporate homologous DNA into their chromosomes by natural transformation. Transformation in Acinetobacter exhibits several unique properties that indicate this system's superiority as a model for transformation studies or studies which benefit from the use of transformation as an experimental method

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16

Morris, Alison Claire. "Genetic transformation of the mosquito Aedes aegypti using a transposable genetic element." Thesis, University of Liverpool, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.279703.

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17

au, jiangwu@central murdoch edu, and Jiang Wu. "Transformation of Rhizoctonia solani." Murdoch University, 2003. http://wwwlib.murdoch.edu.au/adt/browse/view/adt-MU20050728.141653.

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The aim of this study was to develop a genetic transformation system for the plant pathogenic fungus Rhizoctonia solani (teliomorph, Thanatephprus cucumeris Frank [Donk]). The availability of a transformation system would allow us to study gene exchange, epidemiology, and to use techniques such as gene disruption or gene silencing to investigate the role of fungal enzymes in pathogenesis. The approach adopted was to use Agrobacterium tumefaciens to transform the fungus as reports in the literature suggested that this was the most efficient and easiest method to use. As a preliminary test, F

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18

Wang, Tsung-Tsan 1959. "Transformant system and gene expression of yeast Schwanniomyces occidentalis." Thesis, McGill University, 1999. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=35955.

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Schwanniomyces occidentalis (Debaryomyces occidentalis ) is able to grow rapidly with high cell mass on cheap starch as a carbon source, produce strong amylolytic enzymes extracellularly and secrete large proteins without hyper-glycosylation and measurable extracellular proteases. Schw. occidentalis thus has a high potential as, a useful alternative to Saccharomyces cerevisiae in the production of heterologous proteins. However, the molecular study of Schw. occidentalis has been very limited due to the insufficient transformation system and lack of gene expression information.<br>A new transfo

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19

Zhou, Chen, and 周辰. "Genome-informed studies on Penicillium marneffei: horizontal gene transfer survey and differentialsecretomics." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2008. http://hub.hku.hk/bib/B41633672.

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20

Iglesias, Victor Alejandro. "Genetic transformation studies in wheat using particle bombardment /." [S.l.] : [s.n.], 1994. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=10628.

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21

Jiang, Liwen. "Somatic embryogenesis and genetic transformation in douglas-fir." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/29882.

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Cell division was obtained from cultured microspores of Douglas-fir on medium supplemented with auxin, cytokinin, and sucrose, but without medium salts. Embryogenic callus was initiated from excised mature and immature zygotic embryos of Douglas-fir on media supplemented with cytokinin and auxin. Precotyledonary embryos produced most of the embryogenic calli in the cultures. Secondary embryogenic callus production, and subsequent subculturing, were required for the establishment of stable embryogenic callus lines for both mature and immature zygotic embryos. Somatic embryos at the precotyledon

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22

Travella, Silvia. "Improving and understanding the barley genetic transformation process." Thesis, University of East Anglia, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365058.

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23

Tan, Chia Lock. "Tissue culture and genetic transformation of Theobroma cacao." Thesis, University of Nottingham, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.310835.

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24

Deljou, Ali. "Tissue culture and genetic transformation in potato breeding." Thesis, University of Nottingham, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.339661.

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25

Owuama, C. I. "Genetic transformation of Saccharomyces cerevisiae with chimaeric plasmids." Thesis, University of Liverpool, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.381362.

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26

Sparrow, Penelope Amelia Claire. "Plant morphogenesis and genetic transformation of horticultural brassicas." Thesis, Open University, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252364.

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27

Tolonen, Andrew Carl. "Prochlorococcus genetic transformation and genomics of nitrogen metabolism." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/43721.

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Thesis (Ph. D.)--Joint Program in Biological Oceanography (Massachusetts Institute of Technology, Dept. of Biology; and the Woods Hole Oceanographic Institution), 2005.<br>Includes bibliographical references.<br>Prochlorococcus, a unicellular cyanobacterium, is the most abundant phytoplankton in the oligotrophic, oceanic gyres where major plant nutrients such as nitrogen (N) and phosphorus (P) are at nanomolar concentrations. Nitrogen availability controls primary productivity in many of these regions. The cellular mechanisms that Prochlorococcus uses to acquire and metabolize nitrogen are thu

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28

Mohandas, Kavitha. "Genetic transformation of the entomopathogenic deuteromycete, Metarhizium flavoviride." Thesis, University of Bath, 1998. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.245885.

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29

Korban, Martine. "Agrobacterium-mediated transformation of common bean (Phaseolus vulgaris L.)." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41644.

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Regeneration and shoot multiplication of common bean (Phaseolus vulgaris L. 'ICA Pijao') from half-cotyledonary nodes was achieved on modified Murashige and Skoog (1962) basal medium amended with 5 $ mu$M 6-benzylaminopurine. Histological studies confirmed the adventitious origin of the regenerated buds. Shoots were rooted ex vitro and developed into morphologically normal plants compared with seed-grown controls. The relative susceptibility of bean tissues to infection by a collection of wild-type Agrobacterium strains was tested. Positive transformation events were evaluated based on morphol

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30

Becker, Eric Christian. "Recognition of oriT at the termination of conjugal transfer by MobA, the R1162 DNA strand transferase." Access restricted to users with UT Austin EID Full text (PDF) from UMI/Dissertation Abstracts International, 2002. http://wwwlib.umi.com/cr/utexas/fullcit?p3077405.

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31

Van, Eeden C. (Christiaan). "The construction of gene silencing transformation vectors for the introduction of multiple-virus resistance in grapevines." Thesis, Stellenbosch : Stellenbosch University, 2004. http://hdl.handle.net/10019.1/53764.

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Thesis (MSc)--University of Stellenbosch, 2004.<br>ENGLISH ABSTRACT: Viruses are some of the most important pathogens of grapevines. There are no effective chemical treatments, and no grapevine- or other natural resistance genes have been discovered against grapevine infecting viruses. The primary method of grapevine virus control is prevention by biological indexing and molecular- and serological screening of rootstocks and scions before propagation. Due to the spread of grapevine viruses through insect vectors, and in the case of GRSPaV the absence of serological screening, these metho

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32

梁子明 and Tze-ming Leung. "Immunological studies of a phosphorylcholine-specific antibody using gene transfer techniques." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1994. http://hub.hku.hk/bib/B31233740.

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33

Tiengtum, Pimol. "Towards the genetic manipulation of flower colour in Petunia and Curcuma." Thesis, University of Nottingham, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.269714.

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34

Suso, Henri-Pierre. "Development of a system for the genetic transformation of white lupin (Lupinus albus)." Thesis, University of Reading, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.271196.

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35

Leung, Tze-ming. "Immunological studies of a phosphorylcholine-specific antibody using gene transfer techniques /." [Hong Kong] : University of Hong Kong, 1994. http://sunzi.lib.hku.hk/hkuto/record.jsp?B13762680.

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36

Husnain, Tayyab. "Transformation studies in the forage legume Onobrychis viciifolia." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258236.

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37

Limberis, Maria. "A lentiviral gene transfer vector for the treatment of cystic fibrosis airway disease." Title page, synopsis and list of contents only, 2002. http://web4.library.adelaide.edu.au/theses/09PH/09phl735.pdf.

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"16th September 2002." Accompanying CD contains 2 MPEG clips with accompanying text, and a copy in PDF format of: Recovery of airway cystic fibrosis transmembrane conductance regulator function in mice with cystic fibrosis after single-dose lentivirus-mediated gene transfer / M. Limberis ... [et al.], published in Human gene therapy vol. 13 (2002). Bibliography: leaves xxix-li. This thesis focuses on modulating the physical barriers of the airway epithelium with mild detergents, so as to enhance gene transfer by a HIV-1 based lentivirus vector in vivo. The efficiency of the gene transfer was

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38

Cheng, Wai-sheung, and 鄭偉嫦. "PTEN-PKB in endometriosis and related malignant transformation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2005. http://hub.hku.hk/bib/B45009910.

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39

Zhang, Peng. "Studies on cassava (Manihot esculenta Crantz) transformation: towards genetic improvement /." [S.l.] : [s.n.], 2000. http://e-collection.ethbib.ethz.ch/show?type=diss&nr=13962.

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40

Kridel, Robert. "The genetic basis of transformation and progression in follicular lymphoma." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/57547.

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The clonal evolution theory of cancer has been recognized for decades and follows principles of Darwinian selection, in which there is selection of the fittest clones in an ecosystem that is fundamentally heterogeneous and undergoes selective pressure. Follicular lymphoma (FL) emerges as a prototypical disease in which to study clonal evolution. It is the most common indolent lymphoma and, although the median overall survival largely surpasses 10 years, patients almost invariably experience progressive disease. Furthermore, a subset of FL patients is at risk of early lymphoma-related death due

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41

Lee, Sun K. "Genetic transformation of broccoli and promoter tagging in Brassica species." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1996. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq24027.pdf.

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42

Triggs, Heidi M. "Haploid production and genetic transformation of wheat (Triticum aestivum L.)." Thesis, University of Nottingham, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243689.

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43

Drake, Pascal M. W. "Approaches to the genetic transformation of Sitka spruce (Picea sitchensis)." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320788.

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44

Moore, Ian Robert. "Development of a strategy for genetic transformation of plant mitochondria." Thesis, University of Edinburgh, 1989. http://hdl.handle.net/1842/11183.

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45

Silva, José Rodrigo da. "Improvement of chickpea rhizobia by genetic transformation with symbiosis genes." Doctoral thesis, Universidade de Évora, 2018. http://hdl.handle.net/10174/23169.

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Rhizobia are soil bacteria able to induce the formation of nodules in leguminous plants and convert atmospheric nitrogen into assimilable forms to these plants. Some Mesorhizobium species establish symbiosis with chickpea and can increase productivity of this culture. Rhizobia symbiosis genes, such as nod and nif, are involved in nodule development and nitrogen fixation. Nevertheless, genes involved in other molecular mechanisms, namely stress response may influence the symbiotic interaction plantrhizobia. The objective of this study was to evaluate the effects of overexpressing symbiotic and

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46

Burns, Kevin Andrew. "Genetic Detection of Neurogenesis and Astrocytic Transformation of Radial Glia." University of Cincinnati / OhioLINK, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1195153606.

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47

Pavlova, Natalya Nickolayevna. "A Role for PVRL4-Driven Cell-Cell Interactions in Tumorigenesis." Thesis, Harvard University, 2012. http://dissertations.umi.com/gsas.harvard:10722.

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Deciphering genetic determinants of tumorigenesis is the greatest challenge and promise of the present-day era of biomedical research. As extensive tumor genome characterization efforts of the past decade had revealed, tumor genomes harbor multiple point mutations and gene copy number alterations. This exquisite complexity brings forth the challenge of distinguishing numerous incidental alterations from those that are functionally relevant to tumorigenesis. During the past decade, functional genetic screens have shown their utility in identifying genetic changes that functionally contribute to

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48

Davis, Ashley Stuart. "Aspects of rice transformation using direct DNA uptake." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258367.

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Kim, Changhyeon. "Development of a Genetic Transformation System of Raspberry Cultivars for Gene Function Analysis." Thesis, North Dakota State University, 2018. https://hdl.handle.net/10365/29223.

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An Agrobacterium-mediated transformation system of purple raspberry ‘Amethyst’ was established after a series of experiments that determined the effect of genotype, inoculum density, and co-cultivation time on transformation. In this study, a plant regeneration protocol was established for ‘Joan J’ and ‘Polana’ (the regeneration protocol of ‘Amethyst’ was previously developed). Agrobacterium-mediated transformation was conducted for all three cultivars. The minimum killing level of hygromycin B and kanamycin was determined. Inoculum density and co-cultivation time were optimized. Polymerase ch

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50

Canseco-Sedano, Rodolfo. "Factors affecting the efficiency of gene transfer in mice." Diss., This resource online, 1992. http://scholar.lib.vt.edu/theses/available/etd-03172010-020810/.

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