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1

Brown, Gerry, Zeayen Khan, and Ran Lifshitz. "Plant growth promoting rhizobacteria: strain identification by restriction fragment length polymorphisms." Canadian Journal of Microbiology 36, no. 4 (1990): 242–48. http://dx.doi.org/10.1139/m90-042.

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A genomic library of the Pseudomonas putida strain GR12-2 was screened to identify both genus-universal and strain-specific 8-kilobase inserts. The genus-universal clone (pAM141), in combination with the restriction enzymes EcoRI, PstI, and PvuII, was used to generate unique restriction fragment length polymorphisms for 20 related plant growth promoting rhizobacteria and seven reference strains. Strain restriction fragment length polymorphism profiles based on the genus-universal clone pAM141 allow positive identification of individual pseudomonad strains. The strain-specific clone (pAM227) cl
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2

Reddy, Gundlapalli S. N., Genki I. Matsumoto, Peter Schumann, Erko Stackebrandt, and Sisinthy Shivaji. "Psychrophilic pseudomonads from Antarctica: Pseudomonas antarctica sp. nov., Pseudomonas meridiana sp. nov. and Pseudomonas proteolytica sp. nov." International Journal of Systematic and Evolutionary Microbiology 54, no. 3 (2004): 713–19. http://dx.doi.org/10.1099/ijs.0.02827-0.

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Thirty-one bacteria that belonged to the genus Pseudomonas were isolated from cyanobacterial mat samples that were collected from various water bodies in Antarctica. All 31 isolates were psychrophilic; they could be divided into three groups, based on their protein profiles. Representative strains of each of the three groups, namely CMS 35T, CMS 38T and CMS 64T, were studied in detail. Based on 16S rRNA gene sequence analysis, it was established that the strains were related closely to the Pseudomonas fluorescens group. Phenotypic and chemotaxonomic characteristics further confirmed their affi
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3

Blanco-Romero, Esther, Daniel Garrido-Sanz, Rafael Rivilla, Miguel Redondo-Nieto, and Marta Martín. "In Silico Characterization and Phylogenetic Distribution of Extracellular Matrix Components in the Model Rhizobacteria Pseudomonas fluorescens F113 and Other Pseudomonads." Microorganisms 8, no. 11 (2020): 1740. http://dx.doi.org/10.3390/microorganisms8111740.

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Biofilms are complex structures that are crucial during host–bacteria interaction and colonization. Bacteria within biofilms are surrounded by an extracellular matrix (ECM) typically composed of proteins, polysaccharides, lipids, and DNA. Pseudomonads contain a variety of ECM components, some of which have been extensively characterized. However, neither the ECM composition of plant-associated pseudomonads nor their phylogenetic distribution within the genus has been so thoroughly studied. In this work, we use in silico methods to describe the ECM composition of Pseudomonas fluorescens F113, a
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4

Young, J. M., and D. C. Park. "Probable synonymy of the nitrogen-fixing genus Azotobacter and the genus Pseudomonas." International Journal of Systematic and Evolutionary Microbiology 57, no. 12 (2007): 2894–901. http://dx.doi.org/10.1099/ijs.0.64969-0.

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The relationships of the genus Azotobacter, Azomonas macrocytogenes and the genus Pseudomonas were revealed by comparative analysis of partial 16S rRNA and atpD, carA and recA gene sequences and as concatenated nucleotide and peptide sequences. Sequence similarities of Azotobacter species and Azomonas macrocytogenes indicated that these may be considered to be synonyms at the molecular level. In addition, these species show an intimate relationship with species of Pseudomonas, especially P. aeruginosa (the type species of the genus). In terms of the current circumscription of the genus Pseudom
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5

Rivilla, Rafael, and Jacob G. Malone. "Plant-Associated Pseudomonads." Microorganisms 11, no. 5 (2023): 1216. http://dx.doi.org/10.3390/microorganisms11051216.

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Bacteria belonging to the genus Pseudomonas (the pseudomonads) are a group of Gammaproteobacteria that are characterized by a high metabolic versatility and adaption to different ecological niches [...]
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6

Ramos, Elena, Martha-Helena Ramírez-Bahena, Angel Valverde, et al. "Pseudomonas punonensis sp. nov., isolated from straw." International Journal of Systematic and Evolutionary Microbiology 63, Pt_5 (2013): 1834–39. http://dx.doi.org/10.1099/ijs.0.042119-0.

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During a study of the ‘tunta’ (frozen-dry potato) production process in Peru, a bacterial strain, LMT03T, was isolated from the straw grass in which the potatoes are dried. This strain was classified into the genus Pseudomonas on the basis of the 16S rRNA gene sequence analysis, and is most closely related to Pseudomonas argentinensis CH01T with 99.3 % identity in this gene and 96 %, 92 % and 86 % identities in rpoB, rpoD and gyrB genes, respectively. Strain LMT03T has a single polar flagellum, like other related yellow-pigment-producing pseudomonads. The major quinone is Q-9. The major fatty
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7

Bozal, Núria, M. Jesús Montes, and Elena Mercadé. "Pseudomonas guineae sp. nov., a novel psychrotolerant bacterium from an Antarctic environment." International Journal of Systematic and Evolutionary Microbiology 57, no. 11 (2007): 2609–12. http://dx.doi.org/10.1099/ijs.0.65141-0.

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Two Gram-negative, cold-adapted, aerobic bacteria, designated strains M8T and M6, were isolated from soil collected from the South Shetland Islands. The organisms were rod-shaped, catalase- and oxidase-positive and motile by means of polar flagella. These two psychrotolerant strains grew between −4 and 30 °C. 16S rRNA gene sequence analysis placed strains M8T and M6 within the genus Pseudomonas. DNA–DNA hybridization experiments between the Antarctic isolate M8T and type strains of phylogenetically related species, namely Pseudomonas peli and Pseudomonas anguilliseptica, revealed levels of rel
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8

Götze, Sebastian, and Pierre Stallforth. "Structure, properties, and biological functions of nonribosomal lipopeptides from pseudomonads." Natural Product Reports 37, no. 1 (2020): 29–54. http://dx.doi.org/10.1039/c9np00022d.

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Bacteria of the genus Pseudomonas display a fascinating metabolic diversity. In this review, we focus our attention on the natural product class of nonribosomal lipopeptides, which help pseudomonads to colonize a wide range of ecological niches.
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9

Švec, Pavel, Marcel Kosina, Michal Zeman, et al. "Pseudomonas karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves." International Journal of Systematic and Evolutionary Microbiology 70, no. 9 (2020): 5131–40. http://dx.doi.org/10.1099/ijsem.0.004393.

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A taxonomic study of two fluorescent Pseudomonas strains (HJ/4T and SJ/9/1T) isolated from calcite moonmilk samples obtained from two caves in the Moravian Karst in the Czech Republic was carried out. Results of initial 16S rRNA gene sequence analysis assigned both strains into the genus Pseudomonas and showed Pseudomonas yamanorum 8H1T as their closest neighbour with 99.8 and 99.7 % 16S rRNA gene similarities to strains HJ/4T and SJ/9/1T, respectively. Subsequent sequence analysis of rpoD, rpoB and gyrB housekeeping genes confirmed the highest similarity of both isolates to P. yamanorum 8H1T,
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10

Faustova, M. O. "SENSITIVITY OF GRAM-NEGATIVE CAUSATIVE AGENTS OF INFECTIOUS INFLAMMATORY DISEASES OF SOFT TISSUES OF THE FACIAL REGION TO ANTIBIOTICS." Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії 22, no. 3-4 (2022): 169–72. http://dx.doi.org/10.31718/2077-1096.22.3.4.169.

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Today timely monitoring of the sensitivity of gram-negative non-fermenting bacteria taken from various sites of the human body to antibiotics is extremely important in the way of stopping and overcoming significant spread of antibiotic resistance. The aim of this study is to determine the sensitivity of gram-negative causative agents of infectious inflammatory diseases of soft tissues of the face region to antibiotics. Materials and methods. The study included 25 clinical isolates of the genus Acinetobacter and 22 clinical isolates of the genus Pseudomonas. Determination of the sensitivity of
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11

López, Jose R., Ana L. Diéguez, Alejandra Doce, et al. "Pseudomonas baetica sp. nov., a fish pathogen isolated from wedge sole, Dicologlossa cuneata (Moreau)." International Journal of Systematic and Evolutionary Microbiology 62, Pt_4 (2012): 874–82. http://dx.doi.org/10.1099/ijs.0.030601-0.

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Five Gram-negative bacterial isolates, recovered from an outbreak that occurred in March 2006 in Huelva, Spain, affecting adult diseased cultured wedge sole [Dicologlossa cuneata (Moreau)], were characterized phenotypically and genotypically in order to clarify their taxonomic position. On the basis of 16S rRNA gene sequence analysis, the isolates were included in the genus Pseudomonas , within the Pseudomonas fluorescens -related species group, their closest relatives being the Pseudomonas jessenii and Pseudomonas koreensis subgroups. The highest sequence similarities were recorded with the t
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12

Clark, Linda L., Joseph J. Dajcs, Celeste H. McLean, John G. Bartell, and David W. Stroman. "Pseudomonas otitidis sp. nov., isolated from patients with otic infections." International Journal of Systematic and Evolutionary Microbiology 56, no. 4 (2006): 709–14. http://dx.doi.org/10.1099/ijs.0.63753-0.

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A novel Pseudomonas species, for which the name Pseudomonas otitidis sp. nov. is proposed, was identified from clinical specimens of infected human ears. Forty-one pseudomonads (34 from patients with acute otitis externa, six from patients with acute otitis media with otorrhoea and one from a patient with chronic suppurative otitis media) were recovered that did not match any known species. On the basis of genetic analyses and biochemical characterization, these isolates were shown to belong to the genus Pseudomonas. 16S rRNA gene sequence analysis and DNA–DNA hybridization studies indicated t
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13

Hatayama, Kouta, Satomi Kawai, Hirofumi Shoun, Yasuichi Ueda, and Akira Nakamura. "Pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium isolated from a compost pile." International Journal of Systematic and Evolutionary Microbiology 55, no. 4 (2005): 1539–44. http://dx.doi.org/10.1099/ijs.0.63586-0.

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A nitrogen-fixing bacterium, designated strain 6H33bT, was isolated from a compost pile in Japan. The nitrogenase activity of this strain was detected based on its acetylene-reducing activity under low oxygen concentrations (2–4 %). An analysis of the genes responsible for nitrogen fixation in this strain, nifH and nifD, indicated a close relationship to those of Pseudomonas stutzeri A15 (A1501). Sequence similarity searches based on the 16S rRNA gene sequences showed that strain 6H33bT belongs within the genus Pseudomonas sensu stricto; closest similarity was with Pseudomonas indica (97·3 %).
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14

Park, Yoon-Dong, Hana Yi, Keun Sik Baik, et al. "Pseudomonas segetis sp. nov., isolated from soil." International Journal of Systematic and Evolutionary Microbiology 56, no. 11 (2006): 2593–95. http://dx.doi.org/10.1099/ijs.0.63792-0.

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A Gram-negative, aerobic bacterium, designated strain FR1439T, was isolated from the soil of Dokdo in the Republic of Korea. The cells of strain FR1439T were catalase- and oxidase-positive, motile and rod-shaped. Phylogenetic analysis of the 16S rRNA gene sequence revealed that it represents a distinct line of descent within the genus Pseudomonas. The levels of DNA–DNA relatedness between strain FR1439T and type strains of phylogenetically related species, namely Pseudomonas flavescens, Pseudomonas mendocina, Pseudomonas pseudoalcaligenes and Pseudomonas straminea, ranged from 28 to 37 %. Seve
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15

Halpern, Malka, Svetlana Fridman, Yana Aizenberg-Gershtein, and Ido Izhaki. "Transfer of Pseudomonas flectens Johnson 1956 to Phaseolibacter gen. nov., in the family Enterobacteriaceae , as Phaseolibacter flectens gen. nov., comb. nov." International Journal of Systematic and Evolutionary Microbiology 63, Pt_1 (2013): 268–73. http://dx.doi.org/10.1099/ijs.0.033654-0.

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Pseudomonas flectens Johnson 1956, a plant-pathogenic bacterium on the pods of the French bean, is no longer considered to be a member of the genus Pseudomonas sensu stricto. A polyphasic approach that included examination of phenotypic properties and phylogenetic analyses based on 16S rRNA, rpoB and atpD gene sequences supported the transfer of Pseudomonas flectens Johnson 1956 to a new genus in the family Enterobacteriaceae as Phaseolibacter flectens gen. nov., comb. nov. Two strains of Phaseolibacter flectens were studied (ATCC 12775T and LMG 2186); the strains shared 99.8 % sequence simila
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16

Liu, Yang, Qiuhua Rao, Jochen Blom, Qiu Lin, and Tuyan Luo. "Pseudomonas piscis sp. nov., isolated from the profound head ulcers of farmed Murray cod (Maccullochella peelii peelii)." International Journal of Systematic and Evolutionary Microbiology 70, no. 4 (2020): 2732–39. http://dx.doi.org/10.1099/ijsem.0.004101.

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A Gram-stain-negative, strictly aerobic, motile, rod-shaped bacterium with monopolar flagella, designated as MC042T, was isolated from the profound head ulcers of farmed Murray cod sampled from Zhejiang Province, China. Analysis of its 16S rRNA gene sequence and multilocus sequence analysis phylogeny showed that strain MC042T belonged to the genus Pseudomonas , showing the highest 16S rRNA gene sequence similarity to Pseudomonas juntendi BML3T (98.9 %), and less than 98.8 % similarity to other Pseudomonas species with validly published names. Whole-genome sequencing and phylogenetic reconstruc
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17

BENSLAMA, Ouided, and Nedjwa MANSOURI. "Analysis of antibiotic resistance genes in Pseudomonas strains associated with plants: A computational investigation." Notulae Scientia Biologicae 14, no. 1 (2022): 10938. http://dx.doi.org/10.15835/nsb14110938.

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The species of the genus Pseudomonas are an important part of the microbiota associated with plants. These species can be beneficial to the host plant by promoting its growth, and by protecting it against diseases, but they can also have a phytopathogenic effect. The genus Pseudomonas and especially the species P. aeruginosa is classified among the pathogenic species, that are multi-resistant to antibiotics due to the possession of a large number of antibiotic resistance genes (ARGs). Therefore, the risk of contamination of crops by these genes is real and likely to present a danger in terms o
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18

Doszhanov, Ye O., Ye K. Ongarbaev, Z. A. Mansurov, A. A. Zhubanova, and Martin Hofrichter. "Bioremedation of Oil and Oil Products Bacterial Species of the Genus Pseudomonas." Eurasian Chemico-Technological Journal 12, no. 2 (2010): 157. http://dx.doi.org/10.18321/ectj39.

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In the article the results of investigation of oxidation processes of crude-oil by bacteria of genus Pseudomonas: Pseudomonas mendocina H-3, Pseudomonas sp. H-7, Pseudomonas stutzeri H-10, Pseudomonas aeruginosa H-14, Pseudomonas alcaligenes H-15 and Pseudomonas sp H-16. It was shown that these microorganisms are capable to oxidize oil’s hydrocarbons and oil products. It means that the bacteria may be used for bioremediation of oil-pollutant soils.
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19

Kotsoflyak, O. I. "New representatives of Pseudomonas genus from Antarctic soils." Ukrainian Antarctic Journal, no. 4-5 (2006): 214–18. http://dx.doi.org/10.33275/1727-7485.4-5.2006.542.

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Yang, Guiqin, Luchao Han, Junlin Wen, and Shungui Zhou. "Pseudomonas guangdongensis sp. nov., isolated from an electroactive biofilm, and emended description of the genus Pseudomonas Migula 1894." International Journal of Systematic and Evolutionary Microbiology 63, Pt_12 (2013): 4599–605. http://dx.doi.org/10.1099/ijs.0.054676-0.

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A Gram-negative, straight to slightly curved rod-shaped bacterium, motile with peritrichous flagella, designated SgZ-6T, was isolated from an electroactive biofilm and was characterized by means of a polyphasic approach. Growth occurred with 0–5.0 % (w/v) NaCl (optimum 1 %), at pH 6.0–10.0 (optimum pH 7.0) and at 10–42 °C (optimum 30 °C) in trypticase soya broth. Phylogenetic analyses based on the 16S rRNA and gyrB genes identified the isolate as a member of a novel species of the genus Pseudomonas . Strain SgZ-6T exhibited the highest 16S rRNA gene sequence similarity to ‘ Pseudomonas linying
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21

Cabrera, Ma Ángeles, Sebastián L. Márquez, and José M. Pérez-Donoso. "Comparative Genomic Analysis of Antarctic Pseudomonas Isolates with 2,4,6-Trinitrotoluene Transformation Capabilities Reveals Their Unique Features for Xenobiotics Degradation." Genes 13, no. 8 (2022): 1354. http://dx.doi.org/10.3390/genes13081354.

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The nitroaromatic explosive 2,4,6-trinitrotoluene (TNT) is a highly toxic and persistent environmental pollutant. Since physicochemical methods for remediation are poorly effective, the use of microorganisms has gained interest as an alternative to restore TNT-contaminated sites. We previously demonstrated the high TNT-transforming capability of three novel Pseudomonas spp. isolated from Deception Island, Antarctica, which exceeded that of the well-characterized TNT-degrading bacterium Pseudomonas putida KT2440. In this study, a comparative genomic analysis was performed to search for the meta
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22

Hnatiuk, T. T., N. V. Zhitkevich, V. F. Petrychenko, A. V. Kalinichenko, and V. P. Patyka. "Soybean Diseases Caused by Genus Pseudomonas Phytopathenes Bacteria." Mikrobiolohichnyi Zhurnal 81, no. 3 (2019): 68–83. http://dx.doi.org/10.15407/microbiolj81.03.068.

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Ramírez-Bahena, Martha-Helena, Maria José Cuesta, José David Flores-Félix, et al. "Pseudomonas helmanticensis sp. nov., isolated from forest soil." International Journal of Systematic and Evolutionary Microbiology 64, Pt_7 (2014): 2338–45. http://dx.doi.org/10.1099/ijs.0.063560-0.

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A bacterial strain, OHA11T, was isolated during the course of a study of phosphate-solubilizing bacteria occurring in a forest soil from Salamanca, Spain. The 16S rRNA gene sequence of strain OHA11T shared 99.1 % similarity with respect to Pseudomonas baetica a390T, and 98.9 % similarity with the type strains of Pseudomonas jessenii , Pseudomonas moorei , Pseudomonas umsongensis , Pseudomonas mohnii and Pseudomonas koreensis . The analysis of housekeeping genes rpoB, rpoD and gyrB confirmed its phylogenetic affiliation to the genus Pseudomonas and showed similarities lower than 95 % in almost
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24

Asril, Muhammad, and Yuni Lisafitri. "Isolasi Bakteri Pelarut Fosfat Genus Pseudomonas dari Tanah Masam Bekas Areal Perkebunan Karet di Kawasan Institut Teknologi Sumatera." Jurnal Teknologi Lingkungan 21, no. 1 (2020): 40–48. http://dx.doi.org/10.29122/jtl.v21i1.3743.

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ABSTRACTPhosphorus (P) is a nutrient that is needed by plants. The availability of this element is greatly influenced by soil pH. As for ultisol soils classified as acid soils, most of the P in the soil is not available and is bound to Fe and Al. Pseudomonas, a phosphate solubilizing bacteria are soil microbes that can improve the availability of P in acid soils. This study aims to obtain Pseudomonas indigenous, a phosphate solubilizing bacteria from the acid soil formerly used by rubber plantations in the Institut Teknologi Sumatera. The study was conducted from April to June 2018 which inclu
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25

Tao, Yong, Yan Zhou, Xiaohong He, Xiaohong Hu, and Daping Li. "Pseudomonas chengduensis sp. nov., isolated from landfill leachate." International Journal of Systematic and Evolutionary Microbiology 64, Pt_1 (2014): 95–100. http://dx.doi.org/10.1099/ijs.0.050294-0.

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Strain MBRT was isolated from landfill leachate in a solid-waste disposal site in Chengdu, Sichuan, China. An analysis of 16S rRNA gene sequences revealed that the isolate was closely related to members of the genus Pseudomonas , sharing the highest sequence similarities with Pseudomonas toyotomiensis HT-3T (99.8 %), Pseudomonas alcaliphila AL15-21T (99.7 %) and Pseudomonas oleovorans ATCC 8062T (99.4 %). Multi-locus sequence analysis based on three housekeeping genes (gyrB, rpoB and rpoD) provided higher resolution at the species level than that based on 16S rRNA gene sequences, which was fur
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Pascual, Javier, Marina García-López, Gerald F. Bills, and Olga Genilloud. "Pseudomonas granadensis sp. nov., a new bacterial species isolated from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain." International Journal of Systematic and Evolutionary Microbiology 65, Pt_2 (2015): 625–32. http://dx.doi.org/10.1099/ijs.0.069260-0.

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During the course of screening bacterial isolates as sources of as-yet unknown bioactive compounds with pharmaceutical applications, a chemo-organotrophic, Gram-negative bacterium was isolated from a soil sample taken from the Tejeda, Almijara and Alhama Natural Park, Granada, Spain. Strain F-278,770T was oxidase- and catalase-positive, aerobic, with a respiratory type of metabolism with oxygen as the terminal electron acceptor, non-spore-forming and motile by one polar flagellum, although some cells had two polar flagella. Phylogenetic analysis of the 16S rRNA, gyrB, rpoB and rpoD genes revea
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27

Bouteiller, Mathilde, Charly Dupont, Yvann Bourigault, et al. "Pseudomonas Flagella: Generalities and Specificities." International Journal of Molecular Sciences 22, no. 7 (2021): 3337. http://dx.doi.org/10.3390/ijms22073337.

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Flagella-driven motility is an important trait for bacterial colonization and virulence. Flagella rotate and propel bacteria in liquid or semi-liquid media to ensure such bacterial fitness. Bacterial flagella are composed of three parts: a membrane complex, a flexible-hook, and a flagellin filament. The most widely studied models in terms of the flagellar apparatus are E. coli and Salmonella. However, there are many differences between these enteric bacteria and the bacteria of the Pseudomonas genus. Enteric bacteria possess peritrichous flagella, in contrast to Pseudomonads, which possess pol
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Sidorova, T. M., V. V. Allahverdyan, and A. M. Asaturova. "Role of <i>Pseudomonas</i> Bacteria and Their Metabolites in the Biocontrol of Phytopathogenic Microorganisms." Агрохимия, no. 5 (May 1, 2023): 83–93. http://dx.doi.org/10.31857/s0002188123050071.

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The current knowledge on the prospects of using bacteria of the genus Pseudomonas as producers of effective microbiological preparations for the biocontrol of phytopathogenic organisms is summarized and analyzed. Rhizospheric pseudomonads are potential objects of agricultural biotechnology due to the presence of physiological and biochemical features necessary for biocontrol and phytostimulation. These features include tolerance to reactive oxygen species, chemotaxis in relation to root exudates, biosynthesis of siderophores and antibiotic metabolites of various nature. Members of the genus Ps
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29

Dankevich, L. A. "Genetic profiling of bacteria belongs to genus Pseudomonas, what affects legumes." Faktori eksperimental'noi evolucii organizmiv 22 (September 9, 2018): 120–25. http://dx.doi.org/10.7124/feeo.v22.935.

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Aim. For the purpose of correct inter species identification and estimation of group’s heterogeneity, the genome fingerprinting of isolated by us and collection "Pseudomonas lupini" strains as well as typical representative of genus, affecting legumes, has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR) methods and method of molecular phylogenetics (UPGMA) were used. Results. The genetic heterogeneity of isolated and collections "Pseudomonas lupini" strains has been estimated. A relationship between isolated and collections "Pseudomonas lupini
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Mustafa, Sameer Midhat, and Maher Abed Suha. "Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 087–98. https://doi.org/10.5281/zenodo.7924953.

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The bacteria&nbsp;<em>Pseudomonas aeruginosa</em>&nbsp;were isolated and diagnosed and compared with&nbsp;<em>Pseudomonas fluorescens</em>&nbsp;for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and&nbsp;<em>Pseudomonas fluorescens</em>&nbsp;and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitativ
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Vanparys, Bram, Kim Heylen, Liesbeth Lebbe, and Paul De Vos. "Pseudomonas peli sp. nov. and Pseudomonas borbori sp. nov., isolated from a nitrifying inoculum." International Journal of Systematic and Evolutionary Microbiology 56, no. 8 (2006): 1875–81. http://dx.doi.org/10.1099/ijs.0.64224-0.

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Sixteen Gram-negative, rod-shaped, non-spore-forming isolates were obtained from a nitrifying inoculum. Analysis of repetitive sequence-based PCR and SDS-PAGE banding patterns, 16S rRNA gene sequence analysis and DNA–DNA hybridizations showed that the isolates belonged to various groups within the genus Pseudomonas. One group of isolates could be assigned to Pseudomonas migulae and a second to Pseudomonas veronii. Two groups could be differentiated genotypically from each other and from all other currently known Pseudomonas species. Analysis of the fatty acid composition and physiological and
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Girard, Léa, Cédric Lood, Monica Höfte, et al. "The Ever-Expanding Pseudomonas Genus: Description of 43 New Species and Partition of the Pseudomonas putida Group." Microorganisms 9, no. 8 (2021): 1766. http://dx.doi.org/10.3390/microorganisms9081766.

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The genus Pseudomonas hosts an extensive genetic diversity and is one of the largest genera among Gram-negative bacteria. Type strains of Pseudomonas are well known to represent only a small fraction of this diversity and the number of available Pseudomonas genome sequences is increasing rapidly. Consequently, new Pseudomonas species are regularly reported and the number of species within the genus is constantly evolving. In this study, whole genome sequencing enabled us to define 43 new Pseudomonas species and provide an update of the Pseudomonas evolutionary and taxonomic relationships. Phyl
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33

Feng, Zhaozhong, Jun Zhang, Xing Huang, Jing Zhang, Minggen Chen, and Shunpeng Li. "Pseudomonas zeshuii sp. nov., isolated from herbicide-contaminated soil." International Journal of Systematic and Evolutionary Microbiology 62, Pt_11 (2012): 2608–12. http://dx.doi.org/10.1099/ijs.0.037796-0.

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A Gram-stain-negative, rod-shaped, non-motile, non-spore-forming bacterium, designated strain BY-1T, was isolated from a soil sample from the city of Qiqihar in Heilongjiang Province, PR China. Strain BY-1T grew optimally at pH 7.0 and 30–35 °C in the presence of 0.5 % (w/v) NaCl. Analysis of 16S rRNA gene sequences revealed that strain BY-1T fell within the radiation of the genus Pseudomonas , and showed highest 16S rRNA gene sequence similarities to Pseudomonas luteola IAM 13000T (99.5 %) and Pseudomonas duriflava HR2T (97.3 %); the levels of sequence similarity with respect to other recogni
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34

Gibello, Alicia, Ana I. Vela, Margarita Martín, et al. "Pseudomonas composti sp. nov., isolated from compost samples." International Journal of Systematic and Evolutionary Microbiology 61, no. 12 (2011): 2962–66. http://dx.doi.org/10.1099/ijs.0.027086-0.

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Two unusual, Gram-negative, catalase- and oxidase-positive rods, designated C2T and C5, were isolated from compost samples. Comparative 16S rRNA gene sequencing studies demonstrated that both isolates were members of the genus Pseudomonas and belonged to the Pseudomonas aeruginosa group. Strain C2T was most closely related to Pseudomonas cuatrocienegasensis 1NT and Pseudomonas borbori R-20821T (97.9 and 97.8 % 16S rRNA gene sequence similarity, respectively). However, phylogenetic analysis based on rpoD gene sequences revealed that both isolates could be discriminated from members of the P. ae
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35

Mohamed, Hams M. A., Sulaiman Mohammed Alnasser, Hanan H. Abd-Elhafeez, Meshal Alotaibi, Gaber El-Saber Batiha та Waleed Younis. "Detection of β-Lactamase Resistance and Biofilm Genes in Pseudomonas Species Isolated from Chickens". Microorganisms 10, № 10 (2022): 1975. http://dx.doi.org/10.3390/microorganisms10101975.

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Bacteria of the genus Pseudomonas are pathogens in both humans and animals. The most prevalent nosocomial pathogen is P. aeruginosa, particularly strains with elevated antibiotic resistance. In this study, a total of eighteen previously identified Pseudomonas species strains, were isolated from chicken. These strains were screened for biofilm formation and antibiotic resistance. In addition, we evaluated clove oil’s effectiveness against Pseudomonas isolates as an antibiofilm agent. The results showed that Pseudomonas species isolates were resistant to most antibiotics tested, particularly tho
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Bueno-Gonzalez, Victoria, Carrie Brady, Sandra Denman, Joël Allainguillaume, and Dawn Arnold. "Pseudomonas kirkiae sp. nov., a novel species isolated from oak in the United Kingdom, and phylogenetic considerations of the genera Pseudomonas, Azotobacter and Azomonas." International Journal of Systematic and Evolutionary Microbiology 70, no. 4 (2020): 2426–34. http://dx.doi.org/10.1099/ijsem.0.004055.

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As the current episode of Acute Oak Decline (AOD) continues to affect native British oak in the United Kingdom, ongoing isolations from symptomatic and healthy oak have yielded a large Pseudomonas species population. These strains could be divided into taxa representing three potential novel species. Recently, two of these taxa were described as novel Pseudomonas species in the Pseudomonas fluorescens lineage. Here, we demonstrate using a polyphasic approach that the third taxon represents another novel Pseudomonas species. The 16S rRNA gene sequencing assigned the strains to the Pseudomonas a
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37

Widmer, Franco, Ramon J. Seidler, Patrick M. Gillevet, Lidia S. Watrud, and George D. Di Giovanni. "A Highly Selective PCR Protocol for Detecting 16S rRNA Genes of the Genus Pseudomonas (Sensu Stricto) in Environmental Samples." Applied and Environmental Microbiology 64, no. 7 (1998): 2545–53. http://dx.doi.org/10.1128/aem.64.7.2545-2553.1998.

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ABSTRACT Pseudomonas species are plant, animal, and human pathogens; exhibit plant pathogen-suppressing properties useful in biological control; or express metabolic versatilities valued in biotechnology and bioremediation. Specific detection ofPseudomonas species in the environment may help us gain a more complete understanding of the ecological significance of these microorganisms. The objective of this study was to develop a PCR protocol for selective detection ofPseudomonas (sensu stricto) in environmental samples. Extensive database searches identified a highly selective PCR primer pair f
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Bertoldi, Simone, Pedro D. M. A. S. Mattos, Carla C. C. R. de Carvalho, et al. "Evaluation of the Robustness Under Alkanol Stress and Adaptability of Members of the New Genus Halopseudomonas." Microorganisms 12, no. 11 (2024): 2116. http://dx.doi.org/10.3390/microorganisms12112116.

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Many species of the genus Pseudomonas are known to be highly tolerant to solvents and other environmental stressors. Based on phylogenomic and comparative genomic analyses, several Pseudomonas species were recently transferred to a new genus named Halopseudomonas. Because of their unique enzymatic machinery, these strains are being discussed as novel biocatalysts in biotechnology. In order to test their growth parameters and stress tolerance, five Halopseudomonas strains were assessed regarding their tolerance toward different n-alkanols (1-butanol, 1-hexanol, 1-octanol, 1-decanol), as well as
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39

Manaia, C. M. "Pseudomonas thermotolerans sp. nov., a thermotolerant species of the genus Pseudomonas sensu stricto." INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 52, no. 6 (2002): 2203–9. http://dx.doi.org/10.1099/ijs.0.02059-0.

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Manaia, Célia M., and Edward R. B. Moore. "Pseudomonas thermotolerans sp. nov., a thermotolerant species of the genus Pseudomonas sensu stricto." International Journal of Systematic and Evolutionary Microbiology 52, no. 6 (2002): 2203–9. http://dx.doi.org/10.1099/00207713-52-6-2203.

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Hauser, Elke, Peter Kämpfer, and Hans-Jürgen Busse. "Pseudomonas psychrotolerans sp. nov." International Journal of Systematic and Evolutionary Microbiology 54, no. 5 (2004): 1633–37. http://dx.doi.org/10.1099/ijs.0.03024-0.

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Three yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterial strains, C36T, C37 and C39, were isolated in the Medical Clinic for Small Animals and Ungulates at the University for Veterinary Medicine in Vienna, Austria. On the basis of 16S rRNA gene sequence similarity, strain C36T was shown to belong to the genus Pseudomonas; Pseudomonas oleovorans DSM 1045T was the nearest relative (99·5 % sequence similarity). Other Pseudomonas species shared &lt;97 % sequence similarity with strain C36T. The presence of Q-9 as the major ubiquinone, the predominance of putrescine and spermi
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42

Mulet, Magdalena, María José Martínez, Margarita Gomila, et al. "Genome-Based Species Diversity Assessment in the Pseudomonas chlororaphis Phylogenetic Subgroup and Proposal of Pseudomonas danubii sp. nov. Isolated from Freshwaters, Soil, and Rhizosphere." Diversity 15, no. 5 (2023): 617. http://dx.doi.org/10.3390/d15050617.

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The Pseudomonas chlororaphis phylogenetic subgroup of species, within the Pseudomonas fluorescens group, currently includes seven bacterial species, all of which have environmental relevance. Phylogenomic analyses help clarify the taxonomy of strains in the group and allow for precise identification. Thirteen antibiotic-resistant strains isolated in a previous study from nine different sampling sites in the Danube River were suspected to represent a novel species and are investigated taxonomically in the present study, together with four other strains isolated from the Woluwe River (Belgium) t
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43

Evseev, P. V., A. S. Gorshkova, N. N. Sykilinda, V. V. Drucker, and K. A. Miroshnikov. "Pseudomonas bacteriophage AN14 a Baikal-borne representative of Yuavirus." Limnology and Freshwater Biology, no. 5 (2020): 1055–66. http://dx.doi.org/10.31951/2658-3518-2020-a-5-1055.

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Siphoviruses with a prolate capsid infecting Pseudomonas sp. were isolated from environmental sources through the world, and were recently defined as a separate taxonomic genus Yuavirus. Besides a distinguishable morphology, the hallmarks of the genus are heavily modified genomic DNA and a lytic infection cycle while putative lysogeny genes are proposed in the genomes. Bacteriophage AN14 was isolated from Lake Baikal ecosystem and was assigned as a member of Yuavirus. We report the biological and morphological features if this phage, as well as the comprehensive reannotation of its genome. Mod
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44

Saati-Santamaría, Zaki, Ezequiel Peral-Aranega, Encarna Velázquez, Raúl Rivas, and Paula García-Fraile. "Phylogenomic Analyses of the Genus Pseudomonas Lead to the Rearrangement of Several Species and the Definition of New Genera." Biology 10, no. 8 (2021): 782. http://dx.doi.org/10.3390/biology10080782.

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Pseudomonas is a large and diverse genus broadly distributed in nature. Its species play relevant roles in the biology of earth and living beings. Because of its ubiquity, the number of new species is continuously increasing although its taxonomic organization remains quite difficult to unravel. Nowadays the use of genomics is routinely employed for the analysis of bacterial systematics. In this work, we aimed to investigate the classification of species of the genus Pseudomonas on the basis of the analyses of the type strains whose genomes are currently available. Based on these analyses, we
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45

MARSHALL, DOUGLAS L., and RONALD H. SCHMIDT. "Growth of Listeria monocytogenes at 10°C in Milk Preincubated with Selected Pseudomonads1." Journal of Food Protection 51, no. 4 (1988): 277–82. http://dx.doi.org/10.4315/0362-028x-51.4.277.

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Preliminary studies involving co-inoculation of Listeria monocytogenes with Pseudomonas fragi into whole or skim milk demonstrated that neither inhibition nor stimulation of growth occurred for either organism. Additional investigations involved preincubation of whole milk, skim milk, and 10% reconstituted nonfat dry milk (NDM) for 3 d at 10°C with P. fragi, Pseudomonas fluorescens P26, P. fluorescens T25, or P. fluorescens B52, followed by inoculation with L. monocytogenes and further incubation at 10°C. Growth curves of L. monocytogenes were constructed for each treatment combination and gen
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46

Toro, Marcia, Martha-Helena Ramírez-Bahena, Maria José Cuesta, Encarna Velázquez, and Alvaro Peix. "Pseudomonas guariconensis sp. nov., isolated from rhizospheric soil." International Journal of Systematic and Evolutionary Microbiology 63, Pt_12 (2013): 4413–20. http://dx.doi.org/10.1099/ijs.0.051193-0.

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We isolated a bacterial strain designated PCAVU11T in the course of a study of phosphate-solubilizing bacteria occurring in rhizospheric soil of Vigna unguiculata (L.) Walp. in Guárico state, Venezuela. The 16S rRNA gene sequence had 99.2 % sequence similarity with respect to the most closely related species, Pseudomonas taiwanensis , and 99.1 % with respect to Pseudomonas entomophila , Pseudomonas plecoglossicida and Pseudomonas monteilii , on the basis of which PCAVU11T was classified as representing a member of the genus Pseudomonas . Analysis of the housekeeping genes rpoB, rpoD and gyrB c
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Mühlbach, HP, H. Tantau, A. Stubbe, et al. "Grouping of Pseudomonas spp. isolated from dieback- affected sissoo (Dalbergia sissoo Roxb.) using phylogenetic analyses." Plant Tissue Culture and Biotechnology 24, no. 2 (2015): 141–53. http://dx.doi.org/10.3329/ptcb.v24i2.23549.

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Dieback of sissoo (Dalbergia sissoo Roxb.) is a devastating plant disease, whose causative biotic agents were not yet identified unequivocally. Our previous studies revealed that bacteria belonging to the genus Pseudomonas were associated with dieback?affected sissoo trees. To study the bacterial community associated with dieback-affected sissoo trees, DNA based approaches were used in the present work. Sequencing almost the complete 16S rRNA gene of 33 selected Pseudomonas isolates allowed them to be grouped into two main clusters only. The major group consisted of 19 isolates related to P. o
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48

Lin, Shih-Yao, Asif Hameed, You-Cheng Liu, et al. "Pseudomonas sagittaria sp. nov., a siderophore-producing bacterium isolated from oil-contaminated soil." International Journal of Systematic and Evolutionary Microbiology 63, Pt_7 (2013): 2410–17. http://dx.doi.org/10.1099/ijs.0.045567-0.

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An aerobic, Gram-stain-negative, rod-shaped bacterium with a single polar flagellum, designated CC-OPY-1T, was isolated from an oil-contaminated site in Taiwan. CC-OPY-1T produces siderophores, and can grow at temperatures of 25–37 °C and pH 5.0–9.0 and tolerate &lt;5 % (w/v) NaCl. The 16S rRNA gene sequence analysis of CC-OPY-1T showed high pairwise sequence similarity to Pseudomonas alcaligenes BCRC 11893T (97.1 %), Pseudomonas. alcaliphila DSM 17744T (97.1 %), Pseudomonas tuomuerensis JCM 14085T (97.1 %), Pseudomonas toyotomiensis JCM 15604T (96.9 %) and lower sequence similarity to remaini
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Wafula, Eliud Nalianya, and Sylvia Injete Murunga. "Isolation and Identification of Phosphate Solubilizing and Nitrogen-Fixing Bacteria from Lake Ol’Bolossat Sediments, Kenya." Modern Applied Science 14, no. 10 (2020): 37. http://dx.doi.org/10.5539/mas.v14n10p37.

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Phosphate solubilizing and nitrogen-fixing bacteria have a vital role in improving soil fertility and reverting adversely affected soil properties. These bacteria could contribute towards sustainable agriculture with a focus on reducing excessive use of commercial fertilizers. This study aimed at investigating autochthonous populations of phosphate solubilizing and nitrogen-fixing bacteria from Lake Ol&amp;rsquo;Bolossat sediments. The total microbial counts ranged between 4.8 x 103 to 8.5 x 105 cfu/ml. A total of 50 bacteria were isolated, 34 were obtained from Pikovskaya&amp;rsquo;s agar med
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Morozova, Vera, Igor Babkin, Alina Mogileva, et al. "The First Pseudomonas Phage vB_PseuGesM_254 Active against Proteolytic Pseudomonas gessardii Strains." Viruses 16, no. 10 (2024): 1561. http://dx.doi.org/10.3390/v16101561.

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Bacteria of the Pseudomonas genus, including the Pseudomonas gessardii subgroup, play an important role in the environmental microbial communities. Psychrotolerant isolates of P. gessardii can produce thermostable proteases and lipases. When contaminating refrigerated raw milk, these bacteria spoil it by producing enzymes resistant to pasteurization. One possible way to prevent spoilage of raw milk is to use Pseudomonas lytic phages specific to undesirable P. gessardii isolates. The first phage, Pseudomonas vB_PseuGesM_254, was isolated and characterized, which is active against several proteo
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