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COIMBRA, Cynthia Gisele de Oliveira. "Produção de celulose bacteriana por Gluconacetobacter xylinus e elaboração de filmes comestíveis." Universidade Federal de Pernambuco, 2015. https://repositorio.ufpe.br/handle/123456789/17462.

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Sandoval, Vargas Diego Esteban. "Evaluación del rendimiento de producción de celulosa bacteriana usando microalgas como fuente sustentable de oxígeno." Tesis, Universidad de Chile, 2017. http://repositorio.uchile.cl/handle/2250/144130.

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Seminario de Título entregado a la Universidad de Chile en cumplimiento parcial de los requisitos para optar al Título de Ingeniero en Biotecnología Molecular<br>La celulosa es un polisacárido que se encuentra presente en la naturaleza, el cual es de importancia mundial, debido a sus propiedades únicas que lo hacen fundamental en aplicaciones industriales tan diversas, como lo son en la producción del papel, en la industria biomédica, en el vestuario, cosméticos, entre otros. No sólo los organismos vegetales son capaces de producir celulosa, sino que también microorganismos como las bacte
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Sandoval, Vargas Diego Esteban. "Evaluación del rendimiento de producción de celulosa bacteriana usando microalgas como fuente sustentable de oxíge." Tesis, Universidad de Chile, 2017. http://repositorio.uchile.cl/handle/2250/145553.

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La celulosa es un polisacárido que se encuentra presente en la naturaleza, el cual es de importancia mundial, debido a sus propiedades únicas que lo hacen fundamental en aplicaciones industriales tan diversas, como lo son en la producción del papel, en la industria biomédica, en el vestuario, cosméticos, entre otros. No sólo los organismos vegetales son capaces de producir celulosa, sino que también microorganismos como las bacterias Acetobacter, entre ellas la bacteria Gluconacetobacter xylinus (G. xylinus), la cual es el organismo más estudiado por el rendimiento de biopelícula produci
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Vieira, Denise Cristina Moretti. "Produção de biofilme (membrana de biocelulose) por Gluconacetobacter xylinus em meio de resíduos de frutas e folhas de chá verde." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/9/9134/tde-19062013-185045/.

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A biomembrana, que é uma membrana de celulose bacteriana (C6H10O5)n formada na superfície do meio de cultivo durante a fermentação acética, foi obtida através do cultivo associado de Gluconacetobacter xylinus (formalmente Acetobacter xylinum) e Saccharomyces cerevisiae em meio de folhas de chá verde, resíduos de frutas (abacaxi, mamão, laranja), resíduos de vegetais (beterraba), vinho e colágeno em condições estáticas a 28 ± 2°C de 7 a 30 dias de cultivo. Foi incorporado à biomembrana, extrato hidroalcoólico de Calendula officinalis, devido as suas propriedades anti-inflamatórias, antioxidante
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Hofinger, Michael Dominik [Verfasser], Dirk [Akademischer Betreuer] Weuster-Botz, Tim C. [Akademischer Betreuer] Lüth, and Wolfgang [Akademischer Betreuer] Liebl. "Membrangestützte Herstellung bakterieller Cellulose mit Gluconacetobacter xylinus / Michael Hofinger. Gutachter: Tim C. Lüth ; Wolfgang Liebl. Betreuer: Dirk Weuster-Botz." München : Universitätsbibliothek der TU München, 2011. http://d-nb.info/101959019X/34.

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Vidotto, Francielle Lina. "Produção de celulose e perfil dos metabólitos de fermentação de glicerol associado às fontes glucose e sacarose por Gluconacetobacter xylinus." Universidade Estadual de Londrina. Centro de Ciências Exatas. Programa de Pós-Graduação em Biotecnologia, 2015. http://www.bibliotecadigital.uel.br/document/?code=vtls000207567.

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A celulose bacteriana é dotada de propriedades físicas únicas, incluindo uma rede de fibra ultrafina altamente cristalina. Grande esforço tem sido dedicado a melhorar a produção biotecnológica de celulose e reduzir os custos de produção. A produção de celulose associada ao glicerol é de interesse por este ser o principal subproduto da produção de biodiesel. Devido à sua ampla ocorrência na natureza, muitos microrganismos podem utilizar glicerol como fonte de carbono e energia, desta forma este têm atraído a atenção para o uso na bioconversão do glicerol. Para a biossíntese de celulose utilizou
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Donini, Ígor Augusto Negri [UNESP]. "Desenvolvimento de métodos de cultivo de Gluconacetobacter xylinus para obtenção de compósitos à base de celulose bacteriana e colágeno tipo I adicionado in situ." Universidade Estadual Paulista (UNESP), 2011. http://hdl.handle.net/11449/88021.

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Donini, Ígor Augusto Negri. "Desenvolvimento de métodos de cultivo de Gluconacetobacter xylinus para obtenção de compósitos à base de celulose bacteriana e colágeno tipo I adicionado in situ /." Araraquara : [s.n.], 2011. http://hdl.handle.net/11449/88021.

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Orientador: Reinaldo Marchetto<br>Coorientador: Younés Messaddeq<br>Banca: Wilton Rogério Lustri<br>Banca: Rosana Maria Nascimento de Assunção<br>Resumo: A celulose bacteriana (CB) é um polímero produzido pela bactéria Gluconacetobacter xylinus, e apresenta diversas aplicações biomédicas e tecnológicas. Recentemente foi desenvolvido o método de inserção in situ, que visa a produção de compósitos via adição de materiais no meio de cultura. Neste trabalho tem sido realizada a inserção de colágeno durante a formação da CB no intuito de melhorar as propriedades osteocondutoras do compósito, forman
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Auta, Richard. "Purification and characterisation of novel recombinant β-glucosidases from aspergillus with application in biofuel production". Thesis, University of Wolverhampton, 2015. http://hdl.handle.net/2436/606935.

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β-glucosidases are important components of the cellulase enzyme system in which they not only hydrolyse cellobiose to glucose, but also remove the feedback inhibition effects of cellobiose on exoglucanase and endoglucanase thereby increasing the rate of cellulose degradation to fermentable sugars. A total of 166 proteins were identified as β-glucosidases after manual BLASTp search on the Aspergillus comparative database from eight species. Evidence for Horizontal Gene Transfer (HGT) of bacterial origin of some β-glucosidase genes was provided by their lack of introns, absence of some fungal sp
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Mehta, Kalpa Pravin. "Functional analysis of the acsD gene for understanding cellulose biosynthesis in Gluconacetobacter xylinus." Thesis, 2012. http://hdl.handle.net/2152/ETD-UT-2012-05-5198.

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The acsD gene is a unique gene present in the cellulose biosynthesis operon in G. xylinus. With the use of homologous recombination, the acsD gene disruption mutation was created in the G. xylinus genome. Phenotypic characterization of the acsD gene mutant was investigated with the assistance of light and electron microscopy observations, carboxymethyl cellulose alterations, and lower temperature incubation. The microscopic analysis of the cellulose ribbons secreted from the acsD gene mutant shows that the polymerization and the crystallization components in mutant cells were functional. Obser
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Hsieh, Jung-Ting, and 謝榕庭. "Bacterial Cellulose Production by Gluconacetobacter xylinus." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/35502558594167527705.

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碩士<br>臺灣大學<br>化學工程學研究所<br>98<br>Bacterial cellulose (BC) is a kind of insoluble extracellular polysaccharide produced by Gluconacetobacter strains. Because of the unique properties, mainly the ultrafine reticulated structure, BC has found various applications in paper, textile, and food industries, and as a biomaterial in cosmetics and medicine. In this study, we investigated different cultural methods/conditions and medium composition to understand their influence on the BC production with Gluconacetobacter xylinus. We found that the morphology of BC depends on the cultural methods and compos
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Harripaul, Ricardo Simeon. "Characterization of a novel cellulose biosynthesis inhibitor, CBI28, in Gluconacetobacter xylinus." Thesis, 2010. http://hdl.handle.net/10155/122.

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To study the underlying mechanisms for microbial cellulose biosythesis, a novel compound, CBI28, was used as an inhibitor along with classical genetics and EMS mutagenesis. An EZ-Link Biotin Hydrazide Kit was used to create a CBI28-Biotin conjugate for further studies. Gluconacetobacter xylinus cells were exposed to 10 uM CBI28 to induce cellulose biosythesis inhibition, lysed and small hydrophobic molecules were extracted using methanol and Waters Oasis HLB SPE-Paks. Samples were separated and detected using the Ultra Performance Liquid Chromatograph-Mass Spectrometer/Photo Diode Array. Putat
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Liu, Ren-Han, and 劉人漢. "Effect of cellulose-binding domains on bacterial cellulose production by Gluconacetobacter xylinus." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/23499w.

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碩士<br>國立臺北科技大學<br>化學工程研究所<br>101<br>Bacterial cellulose can be produced by Gram-negative bacterium, such as rod-shaped Gluconacetobacter xylinus. As the organism is obligate aerobic, bacterial cellulose is always produced at the air/liquid interface. In recent years there have been more reports about minimizing the cost of culture and improving the quality of bacterial cellulose. Cellulose-binding domains (CBD) have previously been shown to modulate the elongation of cellulose. In this work, the cellulose-binding domains gene and double cellulose-binding domains (dCBD) were cloned and successf
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Yen, Pei-Yu, and 顏珮羽. "Expression of Hemoglobin by genetic engineering in Gluconacetobacter xylinus and Escherichia coli." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/3869rw.

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碩士<br>國立彰化師範大學<br>生物學系<br>107<br>Vitreoscilla hemoglobin (VHB) has been shown in many previous studies to promote not only the growth of bacteria but also the production of exogenous proteins. Bacterial cellulose produced by Gluconacetobacter xylinus has considerable industrial application value, but at present, it is relatively low in output. In this study, we try to express hemoglobin both in G. xylinus and E. coli, promote the production of bacterial cellulose and 4CL (4-Coumaroyl-CoA ligase) recombinant protein. In the G. xylinus experiment, the hemoglobin did not express after the induced
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Sessler, Tate Hopkins. "Bacterial cellulose in cyanobacteria : enhancement of cellulose production in Synechococcus elongatus with Gluconacetobacter xylinus transgenes." Thesis, 2014. http://hdl.handle.net/2152/28507.

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Bacterial cellulose produced by Gluconacetobacter xylinus possesses an abundance of desirable properties which allow for commercial applications in manufacturing, construction, medicine, as a biofuel feedstock and in electronics as an electrical insulator. Large scale production methods are currently limited by expensive media and requirements for sterile conditions. This thesis investigates the potential for using cellulose synthesized by cyanobacteria as an alternative. Cyanobacteria require fewer media components, fix atmospheric carbon dioxide, and some species fix atmospheric nitrogen, re
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