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Artykuły w czasopismach na temat „Glycolipids”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Wang, Cindy, Engy A. Mahrous, Richard E. Lee, Martha M. Vestling, and Kuni Takayama. "Novel Polyoxyethylene-Containing Glycolipids Are Synthesized inCorynebacterium matruchotiiandMycobacterium smegmatisCultured in the Presence of Tween 80." Journal of Lipids 2011 (2011): 1–12. http://dx.doi.org/10.1155/2011/676535.

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The addition of polyoxyethylene sorbitan monooleate (Tween 80) to a culture of mycobacteria greatly influences cell permeability and sensitivity to antibiotics but very little is known regarding the underlying mechanism. Here we show thatCorynebacterium matruchotii(surrogate of mycobacteria) converts Tween 80 to a structural series of polyoxyethylenic acids which are then used to form novel series-2A and series-2B glycolipids. Minor series-3 glycolipids were also synthesized. The polyoxyethylenic acids replaced corynomycolic acids in the cell wall. Correspondingly the trehalose dicorynomycolat

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2

Symington, F. W., D. L. Hedges, and S. Hakomori. "Glycolipid antigens of human polymorphonuclear neutrophils and the inducible HL-60 myeloid leukemia line." Journal of Immunology 134, no. 4 (1985): 2498–506. http://dx.doi.org/10.4049/jimmunol.134.4.2498.

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Abstract Glycolipid and cell surface carbohydrate antigens of human polymorphonuclear neutrophils (PMN) and of HL-60 myeloid leukemia cells were analyzed with a panel of defined, monoclonal anti-carbohydrate antibodies. Antigenicities of intact PMN, HL-60, and retinoic acid-induced HL-60 (r.a.-HL-60) were studied by flow cytofluorometry. These three cell populations displayed quantitative differences, some of which were induction dependent, in their expression of lactosyl, N-acetyllactosaminyl, Y-hapten (Fuc alpha 1----2Gal beta 1----4(Fuc alpha 1----3)GlcNAc beta 1----R), and sialosyl-X-hapte

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3

Sandhoff, Konrad, and Thomas Kolter. "Biosynthesis and degradation of mammalian glycosphingolipids." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 358, no. 1433 (2003): 847–61. http://dx.doi.org/10.1098/rstb.2003.1265.

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Glycolipids are a large and heterogeneous family of sphingolipids that form complex patterns on eukaryotic cell surfaces. This molecular diversity is generated by only a few enzymes and is a paradigm of naturally occurring combinatorial synthesis. We report on the biosynthetic principles leading to this large molecular diversity and focus on sialic acid–containing glycolipids of the ganglio–series. These glycolipids are particularly concentrated in the plasma membrane of neuronal cells. Their de novo synthesis starts with the formation of the membrane anchor, ceramide, at the endoplasmic retic

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4

Sun, Yingying, Yang Mu, Tianhuan Li, et al. "Extraction, Isolation and Biological Activity of Two Glycolipids from Bangia fusco-purpurea." Marine Drugs 22, no. 4 (2024): 144. http://dx.doi.org/10.3390/md22040144.

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In order to explore the extraction and activity of macroalge glycolipids, six macroalgae (Bangia fusco-purpurea, Gelidium amansii, Gloiopeltis furcata, Gracilariopsis lemaneiformis, Gracilaria sp. and Pyropia yezoensis) glycolipids were extracted with five different solvents firstly. Considering the yield and glycolipids concentration of extracts, Bangia fusco-purpurea, Gracilaria sp. and Pyropia yezoensis were selected from six species of marine macroalgae as the raw materials for the extraction of glycolipids. The effects of the volume score of methanol, solid–liquid ratio, extraction temper

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5

Godavarthy, Padmavathi, and Y. Sunila Kumari. "Glycolipids as Potential Energy Molecules during Starvation in Climbing Perch,Anabas testudineus(Bloch)." International Journal of Zoology 2014 (2014): 1–5. http://dx.doi.org/10.1155/2014/476798.

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Glycolipids are membrane lipids which act as cellular markers and also provide energy for the cells. The present study is an attempt to understand whether glycolipids can act as energy sources during fasting. To achieve this, we selected and subjectedAnabas testudineusto short-term (15 days) and long-term (60 days) laboratory starvation. We estimated glycolipids biochemically using a standard protocol in six different tissues. Results showed a selective decline in glycolipid concentration in certain tissues, and also an increase was observed in some tissues. Short-term fasting led to a decline

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6

Cheng, Janice M. H., Ashna A. Khan, Mattie S. M. Timmer, and Bridget L. Stocker. "Endogenous and Exogenous CD1-Binding Glycolipids." International Journal of Carbohydrate Chemistry 2011 (April 5, 2011): 1–13. http://dx.doi.org/10.1155/2011/749591.

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In the same way that peptide antigens are presented by major histocompatibility complex (MHC) molecules, glycolipid antigens can also activate the immune response via binding to CD1 proteins on antigen-presenting cells (APCs) and stimulate CD1-restricted T cells. In humans, there are five members of the CD1 family, termed CD1a–e, of which CD1a–d are involved in glycolipid presentation at the cell surface, while CD1e is involved in the intracellular trafficking of glycolipid antigens. Both endogenous (self-derived) and exogenous (non-self-derived) glycolipids have been shown to bind to members

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7

Colmenares, M., M. Tiemeyer, P. Kima, and D. McMahon-Pratt. "Biochemical and Biological Characterization of the Protective Leishmania pifanoi Amastigote Antigen P-8." Infection and Immunity 69, no. 11 (2001): 6776–84. http://dx.doi.org/10.1128/iai.69.11.6776-6784.2001.

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ABSTRACT The Leishmania pifanoi amastigote antigen P-8 has been previously shown to induce protective immunity in a murine model of cutaneous leishmaniasis (L. Soong, S. M. Duboise, P. Kima, and D. McMahon-Pratt, Infect. Immun. 63:3559–3566, 1995). As this antigen is of interest for further vaccine studies, the biochemical characterization of P-8 was undertaken. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western-blot analysis, and gel filtration chromatography revealed that P-8 antigen consisted of two proteoglycolipid complexes. The P-8 epitope is associated with theL. pifanoi

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8

Künemund, V., F. B. Jungalwala, G. Fischer, D. K. Chou, G. Keilhauer, and M. Schachner. "The L2/HNK-1 carbohydrate of neural cell adhesion molecules is involved in cell interactions." Journal of Cell Biology 106, no. 1 (1988): 213–23. http://dx.doi.org/10.1083/jcb.106.1.213.

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We investigated whether the L2/HNK-1 carbohydrate epitope, expressed by two unusual glycolipids and several neural adhesion molecules, including L1, neural cell adhesion molecule, J1, and the myelin-associated glycoprotein, is involved in adhesion. Monoclonal L2 antibodies, the L2/HNK-1-reactive, sulfate-3-glucuronyl residue carrying glycolipids (L2 glycolipid) and a tetrasaccharide derived from the L2 glycolipid (L2 tetrasaccharide) were added to microexplant cultures of early postnatal mouse cerebellum, and cell migration and process extension were monitored. On the substrate poly-D-lysine,

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9

WUHRER, Manfred, Sandra RICKHOFF, Roger D. DENNIS, et al. "Phosphocholine-containing, zwitterionic glycosphingolipids of adult Onchocerca volvulus as highly conserved antigenic structures of parasitic nematodes." Biochemical Journal 348, no. 2 (2000): 417–23. http://dx.doi.org/10.1042/bj3480417.

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Human Onchocerca volvulus infection sera were found to recognize zwitterionic glycolipids of O. volvulus and to cross-react with those of other parasitic nematodes (Ascaris suum, Setaria digitata and Litomosoides sigmodontis). By the use of an epitope-specific monoclonal antibody, zwitterionic glycolipids of all these nematode species were observed to contain the antigenic determinant phosphocholine. A hyperimmune serum specific for arthro-series glycolipid structures reacted with the various neutral glycolipids of all these nematodes, which demonstrated that their oligosaccharide moieties bel

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10

Anisuzzaman, Md, Feng Jin, Kamrunnahar Kabery, et al. "Lipid Class and Fatty Acid Compositions of Dried Sea Cucumber Apostichopus japonicus." Open Food Science Journal 11, no. 1 (2019): 79–86. http://dx.doi.org/10.2174/1874256401911010079.

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Introduction: Sea cucumber, Apostichopus japonicus, is becoming popular around the world due to its nutritional and medicinal properties. There are still no detailed chemical studies of the lipid class, glycolipids compositions of sea cucumber. Methods: This study was conducted to determine the lipid class and glycolipid compositions of dried sea cucumber, A. japonicus, and analyze fatty acid compositions of Monogalactosyl Diglycerides (MGDG), Steryl Glycosides (SG) and Sulfoquinovosyl Diglycerides (SQDG). Total lipids of sea cucumber were extracted by Bligh and Dyer method and Sep-Pak Silica

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11

De Libero, Gennaro, and Lucia Mori. "Self Glycosphingolipids: New Antigens Recognized by Autoreactive T Lymphocytes." Physiology 18, no. 2 (2003): 71–76. http://dx.doi.org/10.1152/nips.01418.2002.

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T cells may recognize glycolipids and lipids of bacterial and self origin associated with the CD1 antigen-presenting molecules. Understanding the mechanisms governing CD1-self glycolipid interaction will provide information on the molecular rules of glycolipid presentation and suggest new approaches to immunotherapy.

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12

Alon, R., T. Feizi, C. T. Yuen, R. C. Fuhlbrigge, and T. A. Springer. "Glycolipid ligands for selectins support leukocyte tethering and rolling under physiologic flow conditions." Journal of Immunology 154, no. 10 (1995): 5356–66. http://dx.doi.org/10.4049/jimmunol.154.10.5356.

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Abstract Selectin interactions with glycolipids have been examined previously under static conditions, whereas physiologic interactions mediated by selectins take place under flow. We find that under physiologic flow conditions, sialyl Lewis(x) (sLe(x)) glycolipid and sialyl Lewisa (sLe(a)) neoglycolipid support tethering and rolling adhesions of Chinese hamster ovary (CHO) cells expressing E-selectin and lymphoid and myeloid cells expressing L-selectin. These selectin-mediated adhesions persist at the highest shear stresses that occur in postcapillary venules in vivo and occur at lower site d

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13

Itonori, S., T. Shirai, Y. Kiso, Y. Ohashi, K. Shiota, and T. Ogawa. "Glycosphingolipid composition of rat placenta: changes associated with stage of pregnancy." Biochemical Journal 307, no. 2 (1995): 399–405. http://dx.doi.org/10.1042/bj3070399.

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The composition of glycolipids and their changes in the placenta were investigated in the normal pregnant rat. Total lipid fractions extracted from the placenta between days 12 and 20 of pregnancy (day 0 = oestrus) were subjected to glycolipid analysis using DEAE-Sephadex chromatography, silica-gel HPLC, silica-gel TLC, TLC/immunostaining, matrix-assisted secondary-ion mass spectrometry in the negative-ion mode and 1H NMR. Glycolipids identified in the rat placenta were: gangliosides GM3 (NeuAcLacCer and NeuGcLacCer) and GD3 (NeuAcNeuAcLacCer, NeuAcNeuGcLacCer and NeuGcNeuAcLacCer), and neutra

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14

Fredman, P., L. Mattsson, K. Andersson, et al. "Characterization of the binding epitope of a monoclonal antibody to sulphatide." Biochemical Journal 251, no. 1 (1988): 17–22. http://dx.doi.org/10.1042/bj2510017.

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An IgG1 monoclonal antibody, Sulph I, reacting with sulphatide (3′-sulphogalactosylceramide), was produced by immunizing Balb/c mice with that glycolipid coated on Salmonella minnesota bacterial membrane. Radioimmunodetection of the binding of the monoclonal antibody to structurally related glycolipids adsorbed to microtitre plates or chromatographed on thin-layer plates was used to determine its binding epitope. The antibody showed similar binding avidity to three sulphated glycolipids: sulphatide, sulpholactosylceramide and seminolipid. Lysosulphatide did bind the antibody, but, compared wit

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15

Galili, U., B. A. Macher, J. Buehler, and S. B. Shohet. "Human natural anti-alpha-galactosyl IgG. II. The specific recognition of alpha (1----3)-linked galactose residues." Journal of Experimental Medicine 162, no. 2 (1985): 573–82. http://dx.doi.org/10.1084/jem.162.2.573.

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A natural IgG antibody (anti-Gal) with alpha-galactosyl binding specificity has been found in large amounts (0.5 - 1.0% of serum IgG) in all individuals tested. It has been purified by affinity chromatography on a column of melibiose-Sepharose. In addition to its affinity for normal and pathological senescent human red cells, the antibody readily interacts with rabbit red blood cell (RRBC) glycolipids with alpha-galactosyl terminal residues. Two types (glycosidic linkages of 1----3 vs. 1----4) of rabbit red cells glycolipids with terminal alpha-galactosyl residues were tested for antibody bind

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16

Arino, S., R. Marchal, and J. P. Vandecasteele. "Production of new extracellular glycolipids by a strain ofCellulomonas cellulans(Oerskovia xanthineolytica) and their structural characterization." Canadian Journal of Microbiology 44, no. 3 (1998): 238–43. http://dx.doi.org/10.1139/w97-156.

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Glycolipid-producing bacteria were isolated from soil samples. One of the strains, identified as Cellulomonas cellulans (Oerskovia xanthineolytica), was found to produce significant amounts of unusual extracellular glycolipids, which were shown to be composed of at least 11 individual compounds. Hydrolysis of the glycolipid mixture and gas chromatography - mass spectrometry analysis revealed the presence of fatty acids and hydroxy fatty acids ranging from C10to C18, 16 of which were identified. The glycidic moiety consisted of glucose, rhamnose, and ribose. The same sugars were found to be pre

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17

Shu, Qin, Hanghang Lou, Tianyu Wei, Xiayu Liu, and Qihe Chen. "Contributions of Glycolipid Biosurfactants and Glycolipid-Modified Materials to Antimicrobial Strategy: A Review." Pharmaceutics 13, no. 2 (2021): 227. http://dx.doi.org/10.3390/pharmaceutics13020227.

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Glycolipid biosurfactants are natural amphiphiles and have gained particular interest recently in their biodegradability, diversity, and bioactivity. Microbial infection has caused severe morbidity and mortality and threatened public health security worldwide. Glycolipids have played an important role in combating many diseases as therapeutic agents depending on the self-assembly property, the anticancer and anti-inflammatory properties, and the antimicrobial properties, including antibacterial, antifungal, and antiviral effects. Besides, their role has been highlighted as scavengers in impedi

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18

Laffoon, J., C. Lesch, and C. A. Squier. "A transmission electron microscopic study of porcine stratum corneum treated with topical applications of glycolipid." Proceedings, annual meeting, Electron Microscopy Society of America 44 (August 1986): 266–67. http://dx.doi.org/10.1017/s0424820100142955.

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The mammalian epidermis forms a differentiated surface layer termed the stratum corneum (sc) which represents the principal barrier. It is now known that the permeability barrier is located in the intercellular region of the sc and consists largely of neutral lipids derived from glycolipids that are aligned to form a highly hydrophobic structure. It is possible that this barrier might be augmented by topical application of pure glycolipid, which could diffuse through the regions between the squarnes. We have examined this possibility by treating the backskin of pigs with glycolipids and then p

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19

Galili, Uri. "Conversion of Tumors into Autologous Vaccines by Intratumoral Injection ofα-Gal Glycolipids that Induce Anti-Gal/α-Gal Epitope Interaction". Clinical and Developmental Immunology 2011 (2011): 1–10. http://dx.doi.org/10.1155/2011/134020.

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Anti-Gal is the most abundant antibody in humans, constituting 1% of immunoglobulins. Anti-Gal binds specificallyα-gal epitopes (Galα1-3Galβ1-4GlcNAc-R). Immunogenicity of autologous tumor associated antigens (TAA) is greatly increased by manipulating tumor cells to expressα-gal epitopes and bind anti-Gal. Glycolipids withαgal epitopes (α-gal glycolipids) injected into tumors insert into the tumor cell membrane. Anti-Gal binding to the multiple α-gal epitopesde novopresented on the tumor cells results in targeting of these cells to APC via the interaction between the Fc portion of the bound an

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20

Julián, Esther, Lurdes Matas, José Alcaide, and Marina Luquin. "Comparison of Antibody Responses to a Potential Combination of Specific Glycolipids and Proteins for Test Sensitivity Improvement in Tuberculosis Serodiagnosis." Clinical Diagnostic Laboratory Immunology 11, no. 1 (2004): 70–76. http://dx.doi.org/10.1128/cdli.11.1.70-76.2004.

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ABSTRACT The humoral response to different proteinaceous antigens of Mycobacterium tuberculosis is heterogeneous among patients with active disease, and this has originated in the proposal to use a combination of several specific antigens to find an efficient serodiagnostic test for tuberculosis (TB). However, to date, comparisons of antibody responses to several antigens in the same population have been carried out without consideration of antigenic cell wall glycolipids. In the present study the presence of immunoglobulin G (IgG), IgM, and IgA antibodies to M. tuberculosis glycolipids (sulfo

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21

Dennis, R. D., S. Baumeister, C. Smuda, C. Lochnit, T. Waider, and E. Geyer. "Initiation of chemical studies on the immunoreactive glycolipids of adult Ascaris suum." Parasitology 110, no. 5 (1995): 611–23. http://dx.doi.org/10.1017/s0031182000065331.

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SUMMARYThere is a general lack of basic information concerning one class of glycoconjugate, the glycolipids, from parasitic nematodes. As the prototype, the neutral glycolipid fraction derived from adult males of Ascaris suum was investigated as to its chromatographic, differential chemical staining, antigenic and chemical properties. The thin-layer chromato-graphy-resolved neutral fraction glycolipids could be classified into components of fast and slow migrating band groups. Immunoreactivity was restricted to the latter as detected by IgG and IgM anti-neutral fraction glycolipid antibody lev

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22

Hove, Petronella R., Forgivemore Magunda, Maria Angela de Mello Marques, et al. "Identification and functional analysis of a galactosyltransferase capable of cholesterol glycolipid formation in the Lyme disease spirochete Borrelia burgdorferi." PLOS ONE 16, no. 6 (2021): e0252214. http://dx.doi.org/10.1371/journal.pone.0252214.

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Borrelia burgdorferi (Bb), the etiological agent of Lyme disease, produces a series of simple glycolipids where diacylglycerol and cholesterol serve as the precursor. The cholesterol-based glycolipids, cholesteryl 6-O-acyl-β-D-galactopyranoside (ACGal) and cholesteryl-β-D-galactopyranoside (CGal) are immunogenic and proposed to contribute to the pathogenesis of Lyme disease. Detailed studies of CGal and ACGal in Bb have been hampered by a lack of knowledge of their underlying biosynthetic processes. The genome of Bb encodes four putative glycosyltransferases, and only one of these, BB0572, was

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23

Sun, Yafei, Qin Qin, Ke Song, et al. "Does Sulfoquinovosyl Diacylglycerol Synthase OsSQD1 Affect the Composition of Lipids in Rice Phosphate-Deprived Root?" International Journal of Molecular Sciences 24, no. 1 (2022): 114. http://dx.doi.org/10.3390/ijms24010114.

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Lipids are the essential components of the cell intracellular and plasma membranes. Sulfoquinovosyldiacylglycerol (SQDG) is a glycolipid; glycolipids can replace phospholipids in maintaining phosphate (Pi) homeostasis in plants which are undergoing Pi starvation. Sulfoquinovosyl diacylglycerol synthase 1 (OsSQD1) is a critical enzyme in the first step of catalyzation in the formation of SQDG in rice. In this study, the expression pattern of different zones in roots of OsSQD1 in response to different Pi conditions is examined, and it is found that OsSQD1 is highly expressed in lateral roots und

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24

Nimrichter, Leonardo, Monica M. Burdick, Kazuhiro Aoki, et al. "E-selectin receptors on human leukocytes." Blood 112, no. 9 (2008): 3744–52. http://dx.doi.org/10.1182/blood-2008-04-149641.

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Selectins on activated vascular endothelium mediate inflammation by binding to complementary carbohydrates on circulating neutrophils. The human neutrophil receptor for E-selectin has not been established. We report here that sialylated glycosphingolipids with 5 N-acetyllactosamine (LacNAc, Galβ1-4GlcNAcβ1-3) repeats and 2 to 3 fucose residues are major functional E-selectin receptors on human neutrophils. Glycolipids were extracted from 1010 normal peripheral blood human neutrophils. Individual glycolipid species were resolved by chromatography, adsorbed as model membrane monolayers and selec

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25

Warabi, Kaoru, William T. Zimmerman, Jingkai Shen, et al. "Pachymoside A A novel glycolipid isolated from the marine sponge Pachymatisma johnstonia." Canadian Journal of Chemistry 82, no. 2 (2004): 102–12. http://dx.doi.org/10.1139/v03-183.

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Crude extracts of the North Sea marine sponge Pachymatisma johnstonia showed promising activity in a new assay for inhibitors of bacterial type III secretion. Bioassay-guided fractionation resulted in the isolation of the pachymosides, a new family of sponge glycolipids. A major part of the structural diversity in this family of glycolipids involves increasing degrees of acetylation and differing positions of acetylation on a common pachymoside glycolipid template. All of the metabolites with these variations in acetylation pattern were converted into the same peracetylpachymoside methyl ester

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Leutou, Alain S., Jennifer R. McCall, Robert York, Rajeshwar R. Govindapur, and Andrea J. Bourdelais. "Anti-Inflammatory Activity of Glycolipids and a Polyunsaturated Fatty Acid Methyl Ester Isolated from the Marine Dinoflagellate Karenia mikimotoi." Marine Drugs 18, no. 3 (2020): 138. http://dx.doi.org/10.3390/md18030138.

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A new monogalactosyldiacylglycerol (MGDG), a known monogalactosylmonoacylglycerol (MGMG) and a known polyunsaturated fatty acid methyl ester (PUFAME) were isolated from the marine dinoflagellate Karenia mikimotoi. The planar structure of the glycolipids was elucidated using mass spectroscopy (MS) and nuclear magnetic resonance (NMR) analyses and comparisons to the known glycolipid to confirm its structure. The MGDG was characterized as 3-O-β-D-galactopyranosyl-1-O-3,6,9,12,15-octadecapentaenoyl-2-O-tetradecanoylglycerol 1. The MGMG and PUFAME were characterized as (2S)-3-O-β-D-galactopyranosyl

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Karakosta, Christina, Martina Samiotaki, George Panayotou, Dimitrios Papaconstantinou, and Marilita M. Moschos. "Proteomic Changes of Glycolipid Pathways in Age-Related, Diabetic, and Post-Vitrectomy Cataracts." Journal of Clinical Medicine 13, no. 23 (2024): 7287. https://doi.org/10.3390/jcm13237287.

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Background: Alterations in glycolipid and glycosphingolipid pathways lead to compromised cell membranes and may be involved in cataract formation. However, the exact role of glycolipids in lens opacification is not completely understood. The aim of the current study is to investigate proteome complexity and the role of glycolipid and glycosphingolipid pathways in cataract formation. Methods: The anterior capsule and phacoemulsification (phaco) cassette contents were collected during cataract surgery from eleven participants with diabetic cataract (DC), twelve participants with age-related cata

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Chen, Yeng-Nan, Jung-Tung Hung, Fan-Dan Jan та ін. "Diversity-Oriented Synthesis of a Molecular Library of Immunomodulatory α-Galactosylceramides with Fluorous-Tag-Assisted Purification and Evaluation of Their Bioactivities in Regard to IL-2 Secretion". International Journal of Molecular Sciences 23, № 21 (2022): 13403. http://dx.doi.org/10.3390/ijms232113403.

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Structural variants of α-galactosylceramide (α-GalCer) that stimulate invariant natural killer T (iNKT) cells constitute an emerging class of immunomodulatory agents in development for numerous biological applications. Variations in lipid chain length and/or fatty acids in these glycoceramides selectively trigger specific pro-inflammatory responses. Studies that would link a specific function to a structurally distinct α-GalCer rely heavily on the availability of homogeneous and pure materials. To address this need, we report herein a general route to the diversification of the ceramide portio

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Aspeslagh, Sandrine, Yali Li, Esther Yu та ін. "Functional and structural characterization of potent Th1 biasing 6′-derivatised α-GalCer iNKT cell agonists, and their superior role in tumor protection. (156.4)". Journal of Immunology 186, № 1_Supplement (2011): 156.4. http://dx.doi.org/10.4049/jimmunol.186.supp.156.4.

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Abstract Invariant natural killer T cells are known to have marked immunomodulatory capacity due to their ability to produce copious amounts of effector cytokines. In this study, we report the first crystal structures of a novel class of strong Th1 biasing structural analogues of α-galactosylceramide by addition of aromatic structures on the 6-OH position of galactose. They are characterized by marked Th1 polarized cytokine patterns that are highly conserved between mice and men, and marked tumour protection in vivo. The strength of the Th1 response correlates well with enhanced lipid binding

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Moriguchi, Kota, Yumina Nakamura, Ah-Mee Park, et al. "Anti-Glycolipid Antibody Examination in Five EAE Models and Theiler’s Virus Model of Multiple Sclerosis: Detection of Anti-GM1, GM3, GM4, and Sulfatide Antibodies in Relapsing-Remitting EAE." International Journal of Molecular Sciences 24, no. 16 (2023): 12937. http://dx.doi.org/10.3390/ijms241612937.

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Anti-glycolipid antibodies have been reported to play pathogenic roles in peripheral inflammatory neuropathies, such as Guillain–Barré syndrome. On the other hand, the role in multiple sclerosis (MS), inflammatory demyelinating disease in the central nervous system (CNS), is largely unknown, although the presence of anti-glycolipid antibodies was reported to differ among MS patients with relapsing-remitting (RR), primary progressive (PP), and secondary progressive (SP) disease courses. We investigated whether the induction of anti-glycolipid antibodies could differ among experimental MS models

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Schapiro, Florencia B., Clifford Lingwood, Wendy Furuya, and Sergio Grinstein. "pH-independent retrograde targeting of glycolipids to the Golgi complex." American Journal of Physiology-Cell Physiology 274, no. 2 (1998): C319—C332. http://dx.doi.org/10.1152/ajpcell.1998.274.2.c319.

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A small fraction of the molecules internalized by endocytosis reaches the Golgi complex through a retrograde pathway that is poorly understood. In the present work, we used bacterial toxins to study the retrograde pathway in Vero cells. The recombinant B subunit of verotoxin 1B (VT1B) was labeled with fluorescein to monitor its progress within the cell by confocal microscopy. This toxin, which binds specifically to the glycolipid globotriaosyl ceramide, entered endosomes by both clathrin-dependent and -independent pathways, reaching the Golgi complex. Once internalized, the toxin-receptor comp

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Ariga, T., R. V. Tao, B. C. Lee, et al. "Glycolipid composition of human cataractous lenses. Characterization of Lewisx glycolipids." Journal of Biological Chemistry 269, no. 4 (1994): 2667–75. http://dx.doi.org/10.1016/s0021-9258(17)41996-x.

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Hollenbach, Rebecca, Delphine Muller, André Delavault, and Christoph Syldatk. "Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor." Catalysts 12, no. 5 (2022): 551. http://dx.doi.org/10.3390/catal12050551.

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Glycolipids are a class of biodegradable biosurfactants that are non-toxic and based on renewables, making them a sustainable alternative to petrochemical surfactants. Enzymatic synthesis allows a tailor-made production of these versatile compounds using sugar and fatty acid building blocks with rationalized structures for targeted applications. Therefore, glycolipids can be comprehensively designed to outcompete conventional surfactants regarding their physicochemical properties. However, enzymatic glycolipid processes are struggling with both sugars and fatty acid solubilities in reaction me

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Kostetsky, Eduard, Natalia Chopenko, Maria Barkina, Peter Velansky, and Nina Sanina. "Fatty Acid Composition and Thermotropic Behavior of Glycolipids and Other Membrane Lipids of Ulva lactuca (Chlorophyta) Inhabiting Different Climatic Zones." Marine Drugs 16, no. 12 (2018): 494. http://dx.doi.org/10.3390/md16120494.

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Increasing global temperatures are expected to increase the risk of extinction of various species due to acceleration in the pace of shifting climate zones. Nevertheless, there is no information on the physicochemical properties of membrane lipids that enable the adaptation of the algae to different climatic zones. The present work aimed to compare fatty acid composition and thermal transitions of membrane lipids from green macroalgae Ulva lactuca harvested in the Sea of Japan and the Adriatic Sea in summer. U. lactuca inhabiting the Adriatic Sea had bleached parts of thalli which were complet

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Mahon, Robert N., Roxana E. Rojas, Scott A. Fulton, Jennifer L. Franko, Clifford V. Harding, and W. Henry Boom. "Mycobacterium tuberculosis Cell Wall Glycolipids Directly Inhibit CD4+ T-Cell Activation by Interfering with Proximal T-Cell-Receptor Signaling." Infection and Immunity 77, no. 10 (2009): 4574–83. http://dx.doi.org/10.1128/iai.00222-09.

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ABSTRACT Immune evasion is required for Mycobacterium tuberculosis to survive in the face of robust adaptive CD4+ T-cell responses. We have previously shown that M. tuberculosis can indirectly inhibit CD4+ T cells by suppressing the major histocompatibility complex class II antigen-presenting cell function of macrophages. This study was undertaken to determine if M. tuberculosis could directly inhibit CD4+ T-cell activation. Murine CD4+ T cells were purified from spleens by negative immunoaffinity selection followed by flow sorting. Purified CD4+ T cells were activated for 16 to 48 h with CD3

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Madassery, J. V., B. Gillard, D. M. Marcus, and M. H. Nahm. "Subpopulations of B cells in germinal centers. III. HJ6, a monoclonal antibody, binds globoside and a subpopulation of germinal center B cells." Journal of Immunology 147, no. 3 (1991): 823–29. http://dx.doi.org/10.4049/jimmunol.147.3.823.

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Abstract To identify surface Ag uniquely expressed on human germinal center B cells, we produced a mouse mAb, HJ6. When tonsillar lymphocytes were examined, HJ6 did not label T cells and labeled only about half of PNA+ B cells that were HK23-. HJ6 did not label mononuclear cells from peripheral blood, splenocytes, and any of 29 cell lines including 23 B cell lines. This binding pattern of HJ6 was very similar to that of a mAb named 5B5. It was shown previously that 5B5 bound a glycolipid named CTH (CD77) and its Ag was expressed on HK23- PNA+ tonsillar lymphocytes and Burkitt's lymphoma cell l

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Nagahori, Noriko, Kenichi Niikura, Reiko Sadamoto, Kenji Monde, and Shin-Ichiro Nishimura. "Synthesis of Photopolymerizable Glycolipids and their Application as Scaffolds to Immobilize Proteins with a Micron-Sized Pattern." Australian Journal of Chemistry 56, no. 6 (2003): 567. http://dx.doi.org/10.1071/ch02182.

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Photopolymerizable glycolipids incorporating ceramide- or amido-type linkers and able to form stable monolayers were efficiently synthesized by chemical and enzymatic methods. Glycolipid polymer films served as platforms for the immobilization of proteins through specific carbohydrate–protein interactions at the air–water interface. Carbohydrate-binding proteins deposited on the glycolipid film were observed by atomic force microscopy, which showed varying submicron-sized protein patterns such as dendrites, dots, and networks, depending on the lipid structure, membrane preparation process, and

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Wiegandt, Herbert. "Insect glycolipids." Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism 1123, no. 2 (1992): 117–26. http://dx.doi.org/10.1016/0005-2760(92)90101-z.

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Delavault, André, Katarina Ochs, Olga Gorte, Christoph Syldatk, Erwann Durand, and Katrin Ochsenreither. "Microwave-Assisted One-Pot Lipid Extraction and Glycolipid Production from Oleaginous Yeast Saitozyma podzolica in Sugar Alcohol-Based Media." Molecules 26, no. 2 (2021): 470. http://dx.doi.org/10.3390/molecules26020470.

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Glycolipids are non-ionic surfactants occurring in numerous products of daily life. Due to their surface-activity, emulsifying properties, and foaming abilities, they can be applied in food, cosmetics, and pharmaceuticals. Enzymatic synthesis of glycolipids based on carbohydrates and free fatty acids or esters is often catalyzed using certain acyltransferases in reaction media of low water activity, e.g., organic solvents or notably Deep Eutectic Systems (DESs). Existing reports describing integrated processes for glycolipid production from renewables use many reaction steps, therefore this st

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Petrakova, D. O., M. S. Savchenko, I. S. Popova, et al. "Synthesis of Synthethic Analogs of Glycolipids Containing A (Type 2) Tetrasaccharide." Биоорганическая химия 49, no. 4 (2023): 422–33. http://dx.doi.org/10.31857/s013234232304036x.

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Glycolipids are the components of cellular membrane containing glycan and lipid parts. Transport of glycolipids from membrane and vice versa from extracellular space into the membrane is possible. This opens opportunity for modification of cellular membrane via embedding of glycolipids. In practice, synthetical analogs of glycolipids are significantly more convenient than natural glycolipids for such application, as the properties of the synthetical analogs can be varied and other bioactive components besides glycans can be introduced into their structure. This research describes synthesis of

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Gerasimenko, E. O., M. V. Slobodyanik, S. A. Sonin, and P. О. Popkova. "Glycolipids as a promising ingredient in food and pharmaceutical technologies." New Technologies 18, no. 4 (2023): 35–50. http://dx.doi.org/10.47370/2072-0920-2022-18-4-35-50.

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The relevance of the analysis of scientific publications devoted to the study of the composition, properties, methods of preparation, areas of application, as well as the qualitative and quantitative identification of glycolipids is determined by the prospects for their use as alternative natural surfactants. Glycolipids possessing comparable surfactant properties with widely used surfactants of a petrochemical nature, and distinguished by the absence of toxicity and environmental friendliness, exhibit pronounced physiological and functional properties.Currently, there are no systematic data c

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Ziegler, M., S. Teneberg, S. Witt, et al. "Islet beta-cytotoxic monoclonal antibody against glycolipids in experimental diabetes induced by low dose streptozotocin and Freund's adjuvant." Journal of Immunology 140, no. 12 (1988): 4144–50. http://dx.doi.org/10.4049/jimmunol.140.12.4144.

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Abstract Diabetes was induced in BALB/c mice by four injections of a subdiabetogenic dose (40 mg/kg) of streptozotocin in combination with CFA. The treatment increased the plasma glucose from 5.8 +/- 0.1 to 22.1 +/- 1.3 mmol/liter (n = 9). The diabetic animals had circulating islet cell surface antibodies (75%), and a monoclonal islet cell surface IgM antibody, K56aF3, generated from one of the diabetic BALB/c mice, mediated C-Dependent cytotoxicity against insulin-producing cells and inhibited glucose-stimulated insulin release from isolated rat islets. Solid phase assay on thin layer chromat

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Hartmann, Evamarie, Clifford A. Lingwood, and Joachim Reidl. "Heat-Inducible Surface Stress Protein (Hsp70) Mediates Sulfatide Recognition of the Respiratory Pathogen Haemophilus influenzae." Infection and Immunity 69, no. 5 (2001): 3438–41. http://dx.doi.org/10.1128/iai.69.5.3438-3441.2001.

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ABSTRACT The in vitro glycolipid binding specificity of clinical strains of nontypeable Haemophilus influenzae is altered to include sulfated glycolipids following a brief heat shock. We have constructed, expressed, and purified a recombinant protein of H. influenzae Hsp70, which showed significant specific binding to sulfated galactolipids in vitro. Furthermore, indirect immunofluorescence demonstrates that Hsp70 proteins are surface exposed in H. influenzae only after heat shock and are contained in the outer membrane protein fractions.

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OHKATSU, Yasukazu, Miho OZAWA, Yoshiko NUMATA, and Nobuhiro NAKAMURA. "Glycolipid Enzyme Models. X. Catalysis of Glycolipids with Amino Acid Residue." Journal of Japan Oil Chemists' Society 45, no. 6 (1996): 545–53. http://dx.doi.org/10.5650/jos1996.45.545.

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Yang, Guangli, Richard W. Franck, Hoe-Sup Byun, Robert Bittman, Pranati Samadder, and Gilbert Arthur. "ConvergentC-Glycolipid Synthesis via the Ramberg−Bäcklund Reaction: Active Antiproliferative Glycolipids." Organic Letters 1, no. 13 (1999): 2149–51. http://dx.doi.org/10.1021/ol991211f.

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Weiss, J. B., J. L. Magnani, and M. Strand. "Identification of Schistosoma mansoni glycolipids that share immunogenic carbohydrate epitopes with glycoproteins." Journal of Immunology 136, no. 11 (1986): 4275–82. http://dx.doi.org/10.4049/jimmunol.136.11.4275.

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Abstract The immunoreactivity of sera of infected hosts against glycolipids derived from Schistosoma mansoni eggs, adult male worms, and cercariae was analyzed by immunostaining of glycolipids resolved by high-performance thin-layer chromatography. Eggs contained the greatest number of immunogenic glycolipids and bound the largest proportion of serum antibodies. Virtually all of the immunogenic egg glycolipids were neutrally charged and contained oligosaccharide chains larger in size than five sugar residues. The glycolipids of each developmental stage were shown by use of five monoclonal anti

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Redman, C. A., P. Schneider, A. Mehlert, and M. A. J. Ferguson. "The glycoinositol-phospholipids of Phytomonas." Biochemical Journal 311, no. 2 (1995): 495–503. http://dx.doi.org/10.1042/bj3110495.

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The Phytomonas spp. are trypanosomatid parasites of plants. A polar glycolipid fraction of a Phytomonas sp., isolated from the plant Euphorbia characias and grown in culture, was fractionated into four major glycolipid species (Phy 1-4). The glycolipids were analysed by chemical and enzymic modifications, composition and methylation analyses, electrospray mass spectrometry and microsequencing after HNO2 deamination and NaB3H4 reduction. The water-soluble headgroup of the Phy2 glycolipid was also analysed by 1H NMR. All four glycolipids were shown to be glycoinositol-phospholipids (GIPLs) with

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Burdin, Nicolas, Laurent Brossay, Yasuhiko Koezuka та ін. "Selective Ability of Mouse CD1 to Present Glycolipids: α-Galactosylceramide Specifically Stimulates Vα14+ NK T Lymphocytes". Journal of Immunology 161, № 7 (1998): 3271–81. http://dx.doi.org/10.4049/jimmunol.161.7.3271.

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Abstract Mouse CD1 (mCD1) glycoproteins are known to present peptides, while human CD1 molecules present glycolipids. In mice, mCD1-autoreactive NK T cells play critical roles in various immune responses, through the secretion of high amounts of cytokines. This study was initiated to determine whether glycolipids are involved in the autorecognition of mCD1 by NK T cells. α-Galactosylceramide (α-GalCer) was the only glycolipid tested capable of eliciting an mCD1-restricted response by splenic T cells. Moreover, splenic T cells derived from mCD1-deficient mice were not stimulated by α-GalCer, su

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Spada, Franca M., Yasuhiko Koezuka, and Steven A. Porcelli. "CD1d-restricted Recognition of Synthetic Glycolipid Antigens by Human Natural Killer T Cells." Journal of Experimental Medicine 188, no. 8 (1998): 1529–34. http://dx.doi.org/10.1084/jem.188.8.1529.

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A conserved subset of mature circulating T cells in humans expresses an invariant Vα24-JαQ T cell receptor (TCR)-α chain rearrangement and several natural killer (NK) locus–encoded C-type lectins. These human T cells appear to be precise homologues of the subset of NK1.1+ TCR-α/β+ T cells, often referred to as NK T cells, which was initially identified in mice. Here we show that human NK T cell clones are strongly and specifically activated by the same synthetic glycolipid antigens as have been shown recently to stimulate murine NK T cells. Responses of human NK T cells to these synthetic glyc

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Buxbaum, Laurence. "Leishmania mexicana infection induces antibody responses to parasite surface glycolipids that can induce IL-10 and suppress IL-12 from macrophages (37.25)." Journal of Immunology 184, no. 1_Supplement (2010): 37.25. http://dx.doi.org/10.4049/jimmunol.184.supp.37.25.

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Abstract Chronic disease of mice caused by the protozoan parasite Leishmania mexicana requires IL-10 and FcγRIII. Immune complexes consisting of IgG bound to the surface of Leishmania amastigotes can induce IL-10 and suppress IL-12 production from macrophages. However, the targets of these antibodies and the kinetics of their production are not known. Several groups have attempted, without success, to identify surface proteins on the amastigote form of the parasite to which IgG can bind. Using ELISA and thin layer chromatography (TLC) immunoblot methods we now show that parasite glycoinositol

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