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1

Wang, Jing Han, Hai Zhen Yang, and Feng Wang. "Potential of Mixotrophic Cultivation of Chlorella sorokiniana for Biodiesel Production." Advanced Materials Research 779-780 (September 2013): 1509–13. http://dx.doi.org/10.4028/www.scientific.net/amr.779-780.1509.

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Biodiesel from microalgae provides a promising alternative for biofuel production. Microalgal biodiesel can be produced under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Studies of biodiesel production from microalgae have been reported mostly focusing on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity ofChlorella sorokinianaunder photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was adopted as organic
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Wang, Jing Han, Hai Zhen Yang, and Feng Wang. "Mixotrophic Cultivation of Scenedesmus sp. as Biodiesel Feedstock." Advanced Materials Research 777 (September 2013): 268–73. http://dx.doi.org/10.4028/www.scientific.net/amr.777.268.

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Microalgae are a promising feedstock for biodiesel production. Microalgal biodiesel can be obtained under three major cultivation modes, namely, photoautotrophic, heterotrophic, and mixotrophic cultivation. Reported studies of microalgal biodiesel production are mainly based on photoautotrophic cultivation, mixotrophic cultivation has rarely been researched. This paper compared the biomass productivity, lipid content, and lipid productivity of Scenedesmus sp. under photoautotrophic, heterotrophic, and mixotrophic cultivation. Glucose was added as organic carbon source at five concentrations (0
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3

Wang, Kaixuan, Zhongjie Wang, Yi Ding, et al. "Optimization of Heterotrophic Culture Conditions for the Algae Graesiella emersonii WBG-1 to Produce Proteins." Plants 12, no. 12 (2023): 2255. http://dx.doi.org/10.3390/plants12122255.

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The aim of this study was to improve the protein content and yield of heterotrophic microalgal cultivation and establish a simple, economical, and efficient method for microalgal protein production using the novel green alga, Graesiella emersonii WBG-1, which has not been previously reported for heterotrophic cultivation. Through batch heterotrophic cultivation of this alga, we observed that glucose was the optimal carbon source, while it could not use sucrose as a carbon source. Biomass production and protein content were significantly reduced when sodium acetate was used as the carbon source
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Sedlacek, Christopher J., Susanne Nielsen, Kenneth D. Greis, et al. "Effects of Bacterial Community Members on the Proteome of the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain Is79." Applied and Environmental Microbiology 82, no. 15 (2016): 4776–88. http://dx.doi.org/10.1128/aem.01171-16.

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ABSTRACTMicroorganisms in the environment do not exist as the often-studied pure cultures but as members of complex microbial communities. Characterizing the interactions within microbial communities is essential to understand their function in both natural and engineered environments. In this study, we investigated how the presence of a nitrite-oxidizing bacterium (NOB) and heterotrophic bacteria affect the growth and proteome of the chemolithoautotrophic ammonia-oxidizing bacterium (AOB)Nitrosomonassp. strain Is79. We investigatedNitrosomonassp. Is79 in co-culture withNitrobacter winogradsky
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5

Correia, Nádia, Hugo Pereira, Peter S. C. Schulze, et al. "Heterotrophic and Photoautotrophic Media Optimization Using Response Surface Methodology for the Novel Microalga Chlorococcum amblystomatis." Applied Sciences 13, no. 4 (2023): 2089. http://dx.doi.org/10.3390/app13042089.

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The nutritional requirements of novel microalgal strains are key for their effective cultivation and metabolite content. Therefore, the optimization of heterotrophic and photoautotrophic culture media is crucial for novel Chlorococcum amblystomatis growth. Heterotrophic and photoautotrophic biomass samples were characterized to identify the differences between their heterotrophic and photoautotrophic biomass composition and their biotechnological potential. Media optimization through surface response methodology led to 44.9 and 51.2% increments in C. amblystomatis-specific growth rates under h
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6

Cupo, Adelaide, Simone Landi, Salvatore Morra, et al. "Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom Cyclotella cryptica for EPA Production." Marine Drugs 19, no. 7 (2021): 355. http://dx.doi.org/10.3390/md19070355.

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Recently, the marketable value of ω-3 fatty acid, particularly eicosapentaenoic acid (EPA), increased considering their health effects for human consumption. Microalgae are considered a valuable and “green” source of EPA alternative to fish oils, but considerable efforts are necessary for their exploitation at an industrial level. Due to the high operation costs of photoautotrophic microalgae cultivation, heterotrophic growth represents a promising economic solution. Marine diatoms are the major ecological producers of ω-3 fatty acids. Few species of diatoms are capable to grow in the dark usi
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Gao, Yifan, Yuan Li, Yan Yang, Jia Feng, Li Ji, and Shulian Xie. "Effects of Trophic Modes on the Lipid Accumulation of Parachlorella kessleri TY." Fermentation 9, no. 10 (2023): 891. http://dx.doi.org/10.3390/fermentation9100891.

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Microalgae are considered to have great potential as a source of biodiesel. Currently, algae culture has three different trophic modes, i.e., autotrophic, heterotrophic, and mixotrophic, but not all kinds of algae are suitable for heterotrophic and mixotrophic cultivation. In this study, Parachlorella kessleri TY, screened from the soil of Shanxi Province, was heterotrophically and mixotrophically treated with glucose as an organic carbon source, and the physiological and biochemical levels of its growth and lipid accumulation were measured. The results showed that the highest biomass and biom
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8

Park, Jeong-Eun, Shan Zhang, Thi Hiep Han, and Sun-Jin Hwang. "The Contribution Ratio of Autotrophic and Heterotrophic Metabolism during a Mixotrophic Culture of Chlorella sorokiniana." International Journal of Environmental Research and Public Health 18, no. 3 (2021): 1353. http://dx.doi.org/10.3390/ijerph18031353.

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The contribution ratio of autotrophic and heterotrophic metabolism in the mixotrophic culture of Chlorella sorokiniana (C. sorokiniana) was investigated. At the early stage of mixotrophic growth (day 0–1), autotrophy contributed over 70% of the total metabolism; however, heterotrophy contributed more than autotrophy after day 1 due to the rapid increase in cell density, which had a shading effect in the photo-bioreactor. Heterotrophy continued to have a higher contribution until the available organic carbon was depleted at which point autotrophy became dominant again. Overall, the increase in
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9

Nishimura, Takao, Raghunath Ramu Pachpande, and Tatsuichi Iwamura. "A heterotrophic synchronous culture of Chlorella." Cell Structure and Function 13, no. 3 (1988): 207–15. http://dx.doi.org/10.1247/csf.13.207.

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10

Korozi, Evagelina, Vasiliki Tsagou, Io Kefalogianni, et al. "Continuous Culture of Auxenochlorella protothecoides on Biodiesel Derived Glycerol under Mixotrophic and Heterotrophic Conditions: Growth Parameters and Biochemical Composition." Microorganisms 10, no. 3 (2022): 541. http://dx.doi.org/10.3390/microorganisms10030541.

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As crude glycerol comprises a potential substrate for microalga fermentation and value added products’ biosynthesis, Auxenochlorella protothecoides was grown on it under heterotrophic and mixotrophic conditions and its growth kinetics were evaluated in a continuous system under steady state conditions. Increasing initial glycerol concentration (from 30 to 50 g/L) in the heterotrophic culture led to reduced biomass yield (Yx/S) and productivity (Px), but favored lipid accumulation. Under heterotrophic conditions, the microalga was found to grow better (biomass up to 7.888 g/L) and faster (highe
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11

Puzanskiy, Roman K., Daria A. Romanyuk, Anastasia A. Kirpichnikova, Vladislav V. Yemelyanov, and Maria F. Shishova. "Plant Heterotrophic Cultures: No Food, No Growth." Plants 13, no. 2 (2024): 277. http://dx.doi.org/10.3390/plants13020277.

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Plant cells are capable of uptaking exogenous organic substances. This inherited trait allows the development of heterotrophic cell cultures in various plants. The most common of them are Nicotiana tabacum and Arabidopsis thaliana. Plant cells are widely used in academic studies and as factories for valuable substance production. The repertoire of compounds supporting the heterotrophic growth of plant cells is limited. The best growth of cultures is ensured by oligosaccharides and their cleavage products. Primarily, these are sucrose, raffinose, glucose and fructose. Other molecules such as gl
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12

Azma, Mojtaba, Rosfarizan Mohamad, Raha Abdul Rahim, and Arbakariya B. Ariff. "Improved Protocol for the Preparation of Axenic Culture and Adaptation to Heterotrophic Cultivation." Open Biotechnology Journal 4, no. 1 (2010): 36–46. http://dx.doi.org/10.2174/1874070701004010036.

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The effectiveness of various physical and chemical methods for the removal of contaminants from the microalgae, Tetraselmis suecica, culture was investigated. The information obtained was used as the basis for the development of improved protocol for the preparation of axenic culture to be adapted to heterotrophic cultivation. Repeated centrifugation and rinsing effectively removed the free bacterial contaminants from the microalgae culture while sonication helped to loosen up the tightly attached bacterial contaminants on the microalgae cells. Removal of bacterial spores was accomplished usin
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13

Xiao, Yibo, Xi He, Qi Ma, et al. "Photosynthetic Accumulation of Lutein in Auxenochlorella protothecoides after Heterotrophic Growth." Marine Drugs 16, no. 8 (2018): 283. http://dx.doi.org/10.3390/md16080283.

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In order to enhance lutein accumulation and to explain the reasons for the difference in lutein accumulation under photoautotrophic and heterotrophic conditions, different culture modes and the associated transcriptome profiles were investigated in Auxenochlorella protothecoides. The heterotrophic-photoautotrophic transition culture mode was investigated for lutein accumulation, changing from organic carbon to increase biomass in dark fermentation to irradiation under nitrogen rich conditions. This strategy increased the lutein content 10 times along with chloroplast regeneration and little bi
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14

Wu, Kangping, Yilin Fang, Biyuan Hong, et al. "Enhancement of Carbon Conversion and Value-Added Compound Production in Heterotrophic Chlorella vulgaris Using Sweet Sorghum Extract." Foods 11, no. 17 (2022): 2579. http://dx.doi.org/10.3390/foods11172579.

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High-cost carbon sources are not economical or sustainable for the heterotrophic culture of Chlorella vulgaris. In order to reduce the cost, this study used sweet sorghum extract (SE) and its enzymatic hydrolysate (HSE) as alternative carbon sources for the heterotrophic culture of Chlorella vulgaris. Under the premise of the same total carbon concentration, the value-added product production performance of Chlorella vulgaris cultured in HSE (supplemented with nitrogen sources and minerals) was much better than that in the glucose medium. The conversion rate of the total organic carbon and the
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15

Fu, Yunlei, Lanbo Yi, Shufang Yang, et al. "Light Induction of Seed Culture Accelerates Lutein Accumulation in Heterotrophic Fermentation of Chlorella protothecoides CS-41." Fermentation 9, no. 8 (2023): 768. http://dx.doi.org/10.3390/fermentation9080768.

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Microalgae are recognized as a promising and valuable source of lutein. However, the current two-stage method for lutein production has drawbacks, such as complex operations and a long cultivation time. Additionally, utilizing heterotrophic fermentation to cultivate microalgae for lutein production leads to low lutein content due to the absence of light. In this study, we proposed a novel cultivation method that involves light induction of the seed culture to enhance lutein production during the heterotrophic cultivation phase of Chlorella protothecoides CS-41. To gain comprehensive insights i
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16

Blöthe, Marco, and Eric E. Roden. "Composition and Activity of an Autotrophic Fe(II)-Oxidizing, Nitrate-Reducing Enrichment Culture." Applied and Environmental Microbiology 75, no. 21 (2009): 6937–40. http://dx.doi.org/10.1128/aem.01742-09.

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ABSTRACT 16S rRNA gene libraries from the lithoautotrophic Fe(II)-oxidizing, nitrate-reducing enrichment culture described by Straub et al. (K. L. Straub, M. Benz, B. Schink, and F. Widdel, Appl. Environ. Microbiol. 62:1458-1460, 1996) were dominated by a phylotype related (95% 16S rRNA gene homology) to the autotrophic Fe(II) oxidizer Sideroxydans lithotrophicus. The libraries also contained phylotypes related to known heterotrophic nitrate reducers Comamonas badia, Parvibaculum lavamentivorans, and Rhodanobacter thiooxidans. The three heterotrophs were isolated and found to be capable of onl
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17

Morowvat, Mohammad H., and Younes Ghasemi. "Maximizing Biomass and Lipid Production in Heterotrophic Culture of Chlorella vulgaris: Techno-Economic Assessment." Recent Patents on Food, Nutrition & Agriculture 10, no. 2 (2019): 115–23. http://dx.doi.org/10.2174/2212798410666180911100034.

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Background: Nowadays, chlorophycean microalgae have attained a broad-spectrum attention as a potential candidate for biomass and bioenergy production. Despite their appreciated benefits, one of major problems is their low biomass and lipid productivity. Here we investigated the heterotrophic culture in shake flasks and stirred tank bioreactor to improve the lipid and biomass production in a naturally isolated strain of Chlorella vulgaris. Methods: A naturally isolated C. vulgaris strain was cultivated in BG-11 medium in shake flask and bioreactor. Its biochemical composition and growth kinetic
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18

Şentürk, Tuğba. "Effect of different trophic cultures on the amount of total carbohydrate and chlorophyll of Oscillatoria sp." Aquatic Research 7, no. 3 (2024): 144–54. http://dx.doi.org/10.3153/ar24013.

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Cyanobacteria (blue-green microalgae) is a gram-negative prokaryotic autotroph found in natural waters that plays a pivotal role in biochemical cycles. The present investigation proposed to study the potential of using different concentrations of glucose as the carbon substrate to produce microalgal biomass and biochemical components, such as photosynthetic pigments and total carbohydrates (CH) by Oscillatoria sp. The cyanobacteria were collected, and the isolated colony was found to be Oscillatoria sp., and it was grown in BG-11 medium for mass cultivation. Then, the centrifuged biomass was w
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19

Khanichaidecha, W., A. Nakaruk, K. Ratananikom, R. Eamrat, and F. Kazama. "Heterotrophic nitrification and aerobic denitrification using pure-culture bacteria for wastewater treatment." Journal of Water Reuse and Desalination 9, no. 1 (2018): 10–17. http://dx.doi.org/10.2166/wrd.2018.064.

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Abstract Due to the high water demand and unsustainable water resource, wastewater reclamation and wastewater treatment prior to discharge have become current important issues. Various treatment technologies, such as biological processes, have been improved as alternatives. In this study, the biological nitrogen removal system using pure-culture Bacillus licheniformis was developed and used as an internal treatment unit in an aquarium to improve the effluent quality for water reuse. The efficiencies for NH4-N and total nitrogen (TN) removal and the quality of treated water verified the occurre
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Zhang, Han, Jiaxin Shi, Cuibai Chen, Meng Yang, Jianping Lu, and Baogang Zhang. "Heterotrophic Bioleaching of Vanadium from Low-Grade Stone Coal by Aerobic Microbial Consortium." International Journal of Environmental Research and Public Health 19, no. 20 (2022): 13375. http://dx.doi.org/10.3390/ijerph192013375.

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Bioleaching is a viable method that assists in increasing the vanadium output in an economical and environmentally friendly manner. Most bioleaching is conducted by pure cultures under autotrophic conditions, which frequently require strong acidity and produce acid wastewater. However, little is known about heterotrophic bioleaching of vanadium by mixed culture. This study investigated the bioleaching of vanadium from low-grade stone coal by heterotrophic microbial consortium. According to the results, vanadium was efficiently extracted by the employed culture, with the vanadium recovery perce
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Singh, V., and A. K. Mittal. "Characterization of biofilm of a rotating biological contactor treating synthetic wastewater." Water Science and Technology 66, no. 2 (2012): 429–37. http://dx.doi.org/10.2166/wst.2012.221.

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A four-stage rotating biological contactor (RBC) was designed and operated to treat synthetic wastewater containing 1,000 mg/l chemical oxygen demand (COD) and 112 mg/l NH4+-N. A mixed culture bacterial biofilm was developed consisting of a heterotrophic bacterium Paracoccus pantotrophus, nitrifiers and other heterotrophs. Applying the peculiar characteristics of P. pantotrophus of simultaneous heterotrophic nitrification and aerobic denitrification, high simultaneous removal of carbon and nitrogen could be achieved in the fully aerobic RBC. The microbial community structure of the RBC biofilm
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Xie, Weiying, Xiaojie Li, Huo Xu, et al. "Optimization of Heterotrophic Culture Conditions for the Microalgae Euglena gracilis to Produce Proteins." Marine Drugs 21, no. 10 (2023): 519. http://dx.doi.org/10.3390/md21100519.

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Euglena gracilis is one of the few permitted edible microalgae. Considering consumer acceptance, E. gracilis grown heterotrophically with yellow appearances have wider food industrial applications such as producing meat analogs than green cells. However, there is much room to improve the protein content of heterotrophic culture cells. In this study, the effects of nitrogen sources, temperature, initial pH, and C/N ratios on the protein production of E. gracilis were evaluated under heterotrophic cultivation. These results indicated that ammonium sulfate was the optimal nitrogen source for prot
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Mozumder, Md Salatul Islam, Laurens Goormachtigh, Linsey Garcia-Gonzalez, Heleen De Wever, and Eveline I. P. Volcke. "Modeling pure culture heterotrophic production of polyhydroxybutyrate (PHB)." Bioresource Technology 155 (March 2014): 272–80. http://dx.doi.org/10.1016/j.biortech.2013.12.103.

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Wang, Ru, Ping Zheng, Ya-Juan Xing, et al. "Anaerobic ferrous oxidation by heterotrophic denitrifying enriched culture." Journal of Industrial Microbiology & Biotechnology 41, no. 5 (2014): 803–9. http://dx.doi.org/10.1007/s10295-014-1424-5.

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Charria-Girón, Esteban, Vanessa Amazo, Daniela De Angulo, et al. "Strategy for Managing Industrial Anaerobic Sludge through the Heterotrophic Cultivation of Chlorella sorokiniana: Effect of Iron Addition on Biomass and Lipid Production." Bioengineering 8, no. 6 (2021): 82. http://dx.doi.org/10.3390/bioengineering8060082.

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Microalgae provides an alternative for the valorization of industrial by-products, in which the nutritional content varies substantially and directly affects microalgae system performance. Herein, the heterotrophic cultivation of Chlorella sorokiniana was systematically studied, allowing us to detect a nutritional deficiency other than the carbon source through assessing the oxygen transfer rate for glucose or acetate fermentation. Consequently, a mathematical model of the iron co-limiting effect on heterotrophic microalgae was developed by exploring its ability to regulate the specific growth
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Obayagbona, O. N., A. Dunkwu-Okafor, O. Odigie, and J. U. Oghene. "Biodegradation potentials of edaphic bacterial isolates cultured on Haloxyfop R Methyl ester herbicide-mineral salt medium." Nigerian Journal of Biotechnology 40, no. 2 (2024): 1–10. http://dx.doi.org/10.4314/njb.v40i2.1.

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The heterotrophic and haloxyfop-R methyl ester utilizing bacterial counts associated with top soil samples was evaluated. Three (3) bacterial isolates; Bacillus sp., Micrococcus sp. and Staphylococcus sp. were cultured and screened for their ability to utilize haloxyfop-R methyl ester as sole source of carbon and energy using the turbidimeteric procedure. The growth profiles of two axenic cultures; Bacillus sp. and Micrococcus sp. were determined using the shake flask test. Parameters which included pH, mean viable bacterial counts, optical density and dissolved CO2 were determined during grow
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Gao, Yu Qiang, Qiu Yan Zhang, Li Huang, Lin Wang, and Xu Ya Yu. "The Influence of Various pH Values on Monoraphidium sp. FXY-10 Growth and Lipid Parameters in Autotrophic and Heterotrophic Conditions." Advanced Materials Research 864-867 (December 2013): 60–66. http://dx.doi.org/10.4028/www.scientific.net/amr.864-867.60.

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The influence of pH on Monoraphidium sp. FXY-10 growth, lipid content, lipid yield, biomass yield, and fatty acid composition is studied in autotrophic and heterotrophic conditions. The results reveal that Monoraphidium sp. FXY-10 can grow better in an acidic environment. Under autotrophic and heterotrophic conditions, the culture time is 37 and 9 day, respectively. And the maximum biomass of algal cells is 32 and 367mg/l/d with the lipid content in autotrophic and heterotrophic conditions reached to 49% and 39%, respectively, with high biomass yield, lipid yield, the saturated fatty acid and
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Hršak, Dubravka, and Ana Begonja. "Possible Interactions within a Methanotrophic-Heterotrophic Groundwater Community Able To Transform Linear Alkylbenzenesulfonates." Applied and Environmental Microbiology 66, no. 10 (2000): 4433–39. http://dx.doi.org/10.1128/aem.66.10.4433-4439.2000.

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ABSTRACT The relationships and interactions within a methanotrophic-heterotrophic groundwater community were studied in a closed system (shake culture) in the presence of methane as the primary carbon and energy source and with the addition of the pure linear alkylbenzenesulfonate (LAS) congener 2-[4-(sulfophenyl)]decan as a cometabolic substrate. When cultured under different conditions, this community was shown to be a stable association, consisting of one obligate type II methanotroph and four or five heterotrophs possessing different nutritional and physiological characteristics. The resul
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Velioğlu Tosuner, Zülfiye, and Raziye Öztürk Ürek. "Cultivation of Arthrospira platensis in heterotrophic and mixotrophic conditions with different concentrations of whey." Aquatic Research 5, no. 2 (2022): 146–53. http://dx.doi.org/10.3153/ar22014.

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Wastes left over from human food production is commonly used to produce feed for animals, which is an important issue for a rational utilization of food sources globally, and a topic that attracts researcher for the establishment of best food production management. Whey as a side product from cheese production has great potentials in terms of nutritional value for both human food and animal feed production. This study aimed to investigate the possible use of whey (1, 10 and 30%, v/v) as an external carbon source for mixotrophic and heterotrophic cultivation of the cyanobacterium Arthrospira pl
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Chen, Feng. "High cell density culture of microalgae in heterotrophic growth." Trends in Biotechnology 14, no. 11 (1996): 421–26. http://dx.doi.org/10.1016/0167-7799(96)10060-3.

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Parrow, Matthew W., and JoAnn M. Burkholder. "ESTUARINE HETEROTROPHIC CRYPTOPERIDINIOPSOIDS (DINOPHYCEAE): LIFE CYCLE AND CULTURE STUDIES1." Journal of Phycology 39, no. 4 (2003): 678–96. http://dx.doi.org/10.1046/j.1529-8817.2003.02146.x.

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Jia, Shi Ru, Zhen Ding, Ning Tan, Nan Wang, Pei Pei Han, and Nan Nan Yuan. "Heterotrophic Fed-Batch Cultures of Nostoc flagelliforme and Production of Extracellular Polysaccharides." Advanced Materials Research 518-523 (May 2012): 5468–71. http://dx.doi.org/10.4028/www.scientific.net/amr.518-523.5468.

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Nostoc flagelliforme is a kind of terrestrial cyanobacterium with high economic value. Dissociated cells, which separated from a natural colony of N. flagelliforme, could be cultivated heterotrophically in the darkness on xylose and glucose under fed-batch culture conditions. Growth and extracellular polysaccharides (EPS) production in different cultures are investigated. At harvest time, the cultures contain 1.215 g•L-1 of biomass and 122.5 mg•L-1 of EPS respectively. The gravimetric EPS production rate is 17.5 mg•g-1•day-1, which is 1.65 times higher than previously reported results for hete
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Fruhen, M., E. Christan, W. Gujer, and O. Wanner. "Significance of Spatial Distribution of Microbial Species in Mixed Culture Biofilms." Water Science and Technology 23, no. 7-9 (1991): 1365–74. http://dx.doi.org/10.2166/wst.1991.0589.

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Experimental data from a biofilm reactor, in which two groups of organisms (Nitrifiers and Heterotrophs) compete for dissolved oxygen, were analyzed by a Mixed Culture Biofilm Model. The objective was to investigate to what extent and how fast relative abundance and spatial distribution of microbial species in a mixed culture biofilm changed upon variations of the bulk fluid substrate composition, and what the consequences of these changes were for substrate removal. Experimental results showed that within nine days the nitrification rate in a biofilm of constant thickness could change by a fa
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Tisserat, Brent, and Robert Silman. "Growth of Plant Tissue Cultures in Ultra-high Levels of Carbon Dioxide under Autotrophic and Heterotrophic Conditions." HortScience 31, no. 4 (1996): 587e—587. http://dx.doi.org/10.21273/hortsci.31.4.587e.

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A comparative study was undertaken to determine the influence of lighting, carbohydrate concentrations and ultra-high levels of CO2, i.e., >10,000 ppm, on sterile culture growth. Past CO2-sterile studies have confirmed that elevation of CO2 to as high as 1000 ppm resulted in beneficial growth. Within special constructed chambers, tissue cultures were given a variety of CO2 levels for 12–16 hours/day using artificial lighting and natural sunlight. Several different plants (lettuce, beans, pine) and plant culture types were grown in CO2-enriched environments, ranging from 350 to 50,000 ppm. I
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Yamamoto-Ikemoto, R., T. Komori, M. Nomuri, Y. Ide, and T. Matsukami. "Nitrogen removal from hydroponic culture wastewater by autotrophic denitrification using thiosulfate." Water Science and Technology 42, no. 3-4 (2000): 369–76. http://dx.doi.org/10.2166/wst.2000.0405.

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Using the upflow biological filter reactor, sulfur denitrification using thiosulfate of hydroponic culture wastewater was examined. Start-up periods of the reactor were one to two weeks. About 90% of nitrogen removal ratio were achieved over 80 days, at 6.3 kg/m3·days of nitrogen loading. Shock loading among 0.56-2.8 kgN/m3· day did not affect the reactor performance. However, when temperature went below 15°C, the effluent characteristics became poor. Suitable S/N and IC/N ratios were calculated as 3.3 and 0.15, respectively. The activities of sulfur denitrification, heterotrophic denitrificat
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36

Gurung, Anirudra, and Ranadhir Chakraborty. "The role ofAcidithiobacillus ferrooxidansin alleviating the inhibitory effect of thiosulfate on the growth of acidophilicAcidiphiliumspecies isolated from acid mine drainage samples from Garubathan, India." Canadian Journal of Microbiology 55, no. 9 (2009): 1040–48. http://dx.doi.org/10.1139/w09-062.

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Several acidophilic chemolithoautotrophic and heterotrophic strains were isolated from acid mine drainage samples from Garubathan, West Bengal, India. The strains, chemolithoautotrophic DK6.1 and heterotrophic DKAP1.1, used in this study were assigned to the species Acidithiobacillus ferrooxidans and Acidiphilium cryptum , respectively. Unamended filtered and subsequently autoclaved elemental sulfur spent medium of A. ferrooxidans was used as the medium to study heterotrophic growth of A. cryptum DKAP1.1. While characterizing the heterotrophic strain, an inhibitory effect of thiosulfate on A.
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37

Okabe, Satoshi, Tomonori Kindaichi, and Tsukasa Ito. "Fate of 14C-Labeled Microbial Products Derived from Nitrifying Bacteria in Autotrophic Nitrifying Biofilms." Applied and Environmental Microbiology 71, no. 7 (2005): 3987–94. http://dx.doi.org/10.1128/aem.71.7.3987-3994.2005.

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ABSTRACT The cross-feeding of microbial products derived from 14C-labeled nitrifying bacteria to heterotrophic bacteria coexisting in an autotrophic nitrifying biofilm was quantitatively analyzed by using microautoradiography combined with fluorescence in situ hybridization (MAR-FISH). After only nitrifying bacteria were labeled with [14C]bicarbonate, biofilm samples were incubated with and without NH4 + as a sole energy source for 10 days. The transfer of 14C originally incorporated into nitrifying bacterial cells to heterotrophic bacteria was monitored with time by using MAR-FISH. The MAR-FI
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38

Wang, Yuxin, Jia Wang, Shufang Yang, et al. "Selecting a preculture strategy for improving biomass and astaxanthin productivity of Chromochloris zofingiensis." Applied Microbiology and Biotechnology 108, no. 1 (2024): 1–17. http://dx.doi.org/10.1007/s00253-023-12873-x.

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Abstract Chromochloris zofingiensis is a potential source of natural astaxanthin; however, its rapid growth and astaxanthin enrichment cannot be achieved simultaneously. This study established autotrophic, mixotrophic, and heterotrophic preculture patterns to assess their ameliorative effect on the C. zofingiensis heterotrophic growth state. In comparison, mixotrophic preculture (MP) exhibited the best improving effect on heterotrophic biomass concentration of C. zofingiensis (up to 121.5 g L−1) in a 20 L fermenter, reaching the global leading level. The astaxanthin productivity achieved 111 m
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39

Dong, Kaiyi, Ying Wang, Wenjing Zhang, and Qian Li. "Prevalence and Preferred Niche of Small Eukaryotes with Mixotrophic Potentials in the Global Ocean." Microorganisms 12, no. 4 (2024): 750. http://dx.doi.org/10.3390/microorganisms12040750.

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Unicellular eukaryotes that are capable of phago-mixotrophy in the ocean compete for inorganic nutrients and light with autotrophs, and for bacterial prey with heterotrophs. In this study, we ask what the overall prevalence of eukaryotic mixotrophs in the vast open ocean is, and how the availability of inorganic nutrients, light, and prey affects their relative success. We utilized the Tara Oceans eukaryotic 18S rRNA gene and environmental context variables dataset to conduct a large-scale field analysis. We also performed isolate-based culture experiments to verify growth and nutritional reso
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40

Orphan, V. J., L. T. Taylor, D. Hafenbradl, and E. F. Delong. "Culture-Dependent and Culture-Independent Characterization of Microbial Assemblages Associated with High-Temperature Petroleum Reservoirs." Applied and Environmental Microbiology 66, no. 2 (2000): 700–711. http://dx.doi.org/10.1128/aem.66.2.700-711.2000.

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ABSTRACT Recent investigations of oil reservoirs in a variety of locales have indicated that these habitats may harbor active thermophilic prokaryotic assemblages. In this study, we used both molecular and culture-based methods to characterize prokaryotic consortia associated with high-temperature, sulfur-rich oil reservoirs in California. Enrichment cultures designed for anaerobic thermophiles, both autotrophic and heterotrophic, were successful at temperatures ranging from 60 to 90°C. Heterotrophic enrichments from all sites yielded sheathed rods (Thermotogales), pleomorphic rods resemblingT
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41

Mével, Geneviève, and Daniel Prieur. "Heterotrophic nitrification by a thermophilicBacillusspecies as influenced by different culture conditions." Canadian Journal of Microbiology 46, no. 5 (2000): 465–73. http://dx.doi.org/10.1139/w00-005.

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The nitrification activity of a thermophilic heterotrophic bacterium, Bacillus MS30 isolated from a deep-sea hydrothermal vent, was studied under various growth conditions. Nitrification was estimated from the nitrogen balance calculations in the culture media. The results showed that this isolate actively nitrified in culture conditions similar to those prevailing in hydrothermal sites. Therefore, its ecological significance was considered. In standard aerobic conditions, MS30 produced nitrite from ammonia and acetate (1.13 µmol NO2-·mg-1dry wt), but nitrate was never produced, and a low nitr
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42

Eze, C. N., I. O. Ogbonna, and J. C. Ogbonna. "Growth, lipids, proteins, and carotenoid contents of some freshwater green microalgae under simulated day/night temperature fluctuation." Nigerian Journal of Biotechnology 39, no. 1 (2022): 9–19. http://dx.doi.org/10.4314/njb.v39i1.2.

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Day/night or seasonal temperature fluctuation is a major factor during outdoor cultivation of microalgae. In the present study, we investigated the impact of simulated day/night temperatures (20oC night and 30oC day) on the growth, lipid, protein and carotenoid contents of four local oleaginous microalgae strains under mixotrophic and heterotrophic conditions. The impact of simulated day/night temperatures on the growth and biochemical compositions varied across species and culture conditions (mixotrophic or heterotrophic). The lipid productivity by Dictyosphaerium sp. under heterotrophic cond
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43

Chen, Feng, and Michael R. Johns. "Heterotrophic growth of Chlamydomonas reinhardtii on acetate in chemostat culture." Process Biochemistry 31, no. 6 (1996): 601–4. http://dx.doi.org/10.1016/s0032-9592(96)00006-4.

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44

Tsavalos, Alexander J., and John G. Day. "Development of media for the mixotrophic/heterotrophic culture ofBrachiomonas submarina." Journal of Applied Phycology 6, no. 4 (1994): 431–33. http://dx.doi.org/10.1007/bf02182162.

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45

Day, John G., and Alexander J. Tsavalos. "An investigation of the heterotrophic culture of the green algaTetraselmis." Journal of Applied Phycology 8, no. 1 (1996): 73–77. http://dx.doi.org/10.1007/bf02186225.

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46

Ohira, Yuichi, Wataru Fukuchi, Masamitsu Shimadzu, and Eiji Obata. "Effect of Organic Substrates on Heterotrophic Culture of Spirulina platensis." KAGAKU KOGAKU RONBUNSHU 35, no. 5 (2009): 553–56. http://dx.doi.org/10.1252/kakoronbunshu.35.553.

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Chen, Feng, and Michael R. Johns. "Substrate inhibition of Chlamydomonas reinhardtii by acetate in heterotrophic culture." Process Biochemistry 29, no. 4 (1994): 245–52. http://dx.doi.org/10.1016/0032-9592(94)80064-2.

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48

Parra-Riofrío, Geovanna, Jorge García-Márquez, Virginia Casas-Arrojo, Eduardo Uribe-Tapia, and Roberto Teófilo Abdala-Díaz. "Antioxidant and Cytotoxic Effects on Tumor Cells of Exopolysaccharides from Tetraselmis suecica (Kylin) Butcher Grown Under Autotrophic and Heterotrophic Conditions." Marine Drugs 18, no. 11 (2020): 534. http://dx.doi.org/10.3390/md18110534.

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Marine microalgae produce extracellular metabolites such as exopolysaccharides (EPS) with potentially beneficial biological applications to human health, especially antioxidant and antitumor properties, which can be increased with changes in crop trophic conditions. This study aimed to develop the autotrophic and heterotrophic culture of Tetraselmis suecica (Kylin) Butcher in order to increase EPS production and to characterize its antioxidant activity and cytotoxic effects on tumor cells. The adaptation of autotrophic to heterotrophic culture was carried out by progressively reducing the phot
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49

JANA, T. K., R. D. BANERJEE, SUSMITA LAHIRI, and B. B. JANA. "Management induced changes of antibiotic resistant strains of heterotrophic bacteria in shrimp farming ponds." Indian Journal of Animal Sciences 84, no. 3 (2014): 323–28. http://dx.doi.org/10.56093/ijans.v84i3.38721.

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Antibiotic resistant strains of heterotrophic bacteria and total aerobic heterotrophic bacteria were enumerated along with important water quality parameters in water and sediment samples collected from 3 shrimp farming ponds used for the monoculture of Penaeus monodon as well as from a control pond located outside the shrimp farm. The resistant strains of heterotrophic bacteria were screened against 3 antibiotics (ampicillin, chloramphenicol and oxytetracycline) at 25 and 50 μg/l. The counts of antibiotic resistant bacteria tended to rise in shrimp ponds with progressive rise in the days of c
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50

Berger, R. G., Z. Akkan, and F. Drawert. "The Essential Oil of Coleonema album (Rutaceae) and of a Photomixotrophic Cell Culture Derived thereof." Zeitschrift für Naturforschung C 45, no. 3-4 (1990): 187–95. http://dx.doi.org/10.1515/znc-1990-3-408.

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Abstract The volatile shoot oil of Coleonema album was found to be composed of monoterpenes, ses­quiterpenes and phenylpropanoids. Heterotrophic and photom ixotrophic callus cultures were established from green parts of the plant. A photom ixotrophic cell line contained a heteroge­neous population of plastids and synthesized an essential oil, which differed qualitatively and quantitatively from the green parts of the intact plant. Substitution of 2,4-D and pCPA by a lower amount of NAA and high kinetin concentrations favoured the formation of essential oil. The heterotrophic callus, when grown
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