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Artykuły w czasopismach na temat „HXK2 deletion”

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Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Petit, Thomas, Jasper A. Diderich, Arthur L. Kruckeberg, Carlos Gancedo, and Karel Van Dam. "Hexokinase Regulates Kinetics of Glucose Transport and Expression of Genes Encoding Hexose Transporters inSaccharomyces cerevisiae." Journal of Bacteriology 182, no. 23 (2000): 6815–18. http://dx.doi.org/10.1128/jb.182.23.6815-6818.2000.

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ABSTRACT Glucose transport kinetics and mRNA levels of different glucose transporters were determined in Saccharomyces cerevisiaestrains expressing different sugar kinases. During exponential growth on glucose, a hxk2 null strain exhibited high-affinity hexose transport associated with an elevated transcription of the genesHXT2 and HXT7, encoding high-affinity transporters, and a diminished expression of the HXT1 andHXT3 genes, encoding low-affinity transporters. Deletion ofHXT7 revealed that the high-affinity component is mostly due to HXT7; however, a previously unidentified very-high-affini

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Diderich, Jasper A., Léonie M. Raamsdonk, Arthur L. Kruckeberg, Jan A. Berden, and Karel Van Dam. "Physiological Properties of Saccharomyces cerevisiae from Which Hexokinase II Has Been Deleted." Applied and Environmental Microbiology 67, no. 4 (2001): 1587–93. http://dx.doi.org/10.1128/aem.67.4.1587-1593.2001.

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ABSTRACT Hexokinase II is an enzyme central to glucose metabolism and glucose repression in the yeast Saccharomyces cerevisiae. Deletion of HXK2, the gene which encodes hexokinase II, dramatically changed the physiology of S. cerevisiae. The hxk2-null mutant strain displayed fully oxidative growth at high glucose concentrations in early exponential batch cultures, resulting in an initial absence of fermentative products such as ethanol, a postponed and shortened diauxic shift, and higher biomass yields. Several intracellular changes were associated with the deletion of hexokinase II. Thehxk2 m

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3

Miskovic, Ljubisa, Susanne Alff-Tuomala, Keng Cher Soh, et al. "A design–build–test cycle using modeling and experiments reveals interdependencies between upper glycolysis and xylose uptake in recombinant S. cerevisiae and improves predictive capabilities of large-scale kinetic models." Biotechnology for Biofuels 10, no. 1 (2017): 166. https://doi.org/10.1186/s13068-017-0838-5.

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<strong>Background: </strong>Recent advancements in omics measurement technologies have led to an ever-increasing amount of available experimental data that necessitate systems-oriented methodologies for efficient and systematic integration of data into consistent large-scale kinetic models. These models can help us to uncover new insights into cellular physiology and also to assist in the rational design of bioreactor or fermentation processes. Optimization and Risk Analysis of Complex Living Entities (ORACLE) framework for the construction of large-scale kinetic models can be used as guidanc

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4

Kümmel, Anne, Jennifer Christina Ewald, Sarah-Maria Fendt, et al. "Differential glucose repression in common yeast strains in response to HXK2 deletion." FEMS Yeast Research 10, no. 3 (2010): 322–32. http://dx.doi.org/10.1111/j.1567-1364.2010.00609.x.

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Amigoni, Loredana, Enzo Martegani, and Sonia Colombo. "Lack ofHXK2Induces Localization of Active Ras in Mitochondria and Triggers Apoptosis in the YeastSaccharomyces cerevisiae." Oxidative Medicine and Cellular Longevity 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/678473.

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We recently showed that activated Ras proteins are localized to the plasma membrane and in the nucleus in wild-type cells growing exponentially on glucose, while in thehxk2Δ strain they accumulated mainly in mitochondria. An aberrant accumulation of activated Ras in these organelles was previously reported and correlated to mitochondrial dysfunction, accumulation of ROS, and cell death. Here we show that addition of acetic acid to wild-type cells results in a rapid recruitment of Ras-GTP from the nucleus and the plasma membrane to the mitochondria, providing a further proof that Ras proteins m

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6

Herwig, Christoph, and Urs von Stockar. "Quantitative analysis of the oxidative metabolism in HXK2- and REG1-deletion mutants of Saccharomyces cerevisiae." Enzyme and Microbial Technology 31, no. 5 (2002): 698–710. http://dx.doi.org/10.1016/s0141-0229(02)00164-3.

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7

Herwig, Christoph, Florentina Chetreanu, Peter Niederberger, Ian Marison, and Urs von Stockar. "Quantitative analysis of the impact of HXK2 and REG1 deletion in Saccharomyces cerevisiae on invertase expression and respiration." Enzyme and Microbial Technology 31, no. 4 (2002): 505–15. http://dx.doi.org/10.1016/s0141-0229(02)00145-x.

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8

Idnurm, Alexander, Steven S. Giles, John R. Perfect, and Joseph Heitman. "Peroxisome Function Regulates Growth on Glucose in the Basidiomycete Fungus Cryptococcus neoformans." Eukaryotic Cell 6, no. 1 (2006): 60–72. http://dx.doi.org/10.1128/ec.00214-06.

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ABSTRACT The function of the peroxisomes was examined in the pathogenic basidiomycete Cryptococcus neoformans. Recent studies reveal the glyoxylate pathway is required for virulence of diverse microbial pathogens of plants and animals. One exception is C. neoformans, in which isocitrate lyase (encoded by ICL1) was previously shown not to be required for virulence, and here this was extended to exclude also a role for malate synthase (encoded by MLS1). The role of peroxisomes, in which the glyoxylate pathway enzymes are localized in many organisms, was examined by mutation of two genes (PEX1 an

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9

Bae, Yi-Hyun, Dae-Hyuk Kweon, Yong-Cheol Park, and Jin-Ho Seo. "Deletion of the HXK2 gene in Saccharomyces cerevisiae enables mixed sugar fermentation of glucose and galactose in oxygen-limited conditions." Process Biochemistry 49, no. 4 (2014): 547–53. http://dx.doi.org/10.1016/j.procbio.2014.01.030.

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10

Van de Velde, Sam, and Johan M. Thevelein. "Cyclic AMP-Protein Kinase A and Snf1 Signaling Mechanisms Underlie the Superior Potency of Sucrose for Induction of Filamentation in Saccharomyces cerevisiae." Eukaryotic Cell 7, no. 2 (2007): 286–93. http://dx.doi.org/10.1128/ec.00276-07.

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ABSTRACT Under specific environmental conditions, the yeast Saccharomyces cerevisiae can undergo a morphological switch to a pseudohyphal growth pattern. Pseudohyphal differentiation is generally studied upon induction by nitrogen limitation in the presence of glucose. It is known to be controlled by several signaling pathways, including mitogen-activated protein kinase, cyclic AMP-protein kinase A (cAMP-PKA), and Snf1 kinase pathways. We show that the alpha-glucoside sugars maltose and maltotriose, and especially sucrose, are more potent inducers of filamentation than glucose. Sucrose even in

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11

Newcomb, Laura L., Jasper A. Diderich, Matthew G. Slattery, and Warren Heideman. "Glucose Regulation of Saccharomyces cerevisiae Cell Cycle Genes." Eukaryotic Cell 2, no. 1 (2003): 143–49. http://dx.doi.org/10.1128/ec.2.1.143-149.2003.

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ABSTRACT Nutrient-limited Saccharomyces cerevisiae cells rapidly resume proliferative growth when transferred into glucose medium. This is preceded by a rapid increase in CLN3, BCK2, and CDC28 mRNAs encoding cell cycle regulatory proteins that promote progress through Start. We have tested the ability of mutations in known glucose signaling pathways to block glucose induction of CLN3, BCK2, and CDC28. We find that loss of the Snf3 and Rgt2 glucose sensors does not block glucose induction, nor does deletion of HXK2, encoding the hexokinase isoenzyme involved in glucose repression signaling. Rap

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12

Heyland, Jan, Jianan Fu, and Lars M. Blank. "Correlation between TCA cycle flux and glucose uptake rate during respiro-fermentative growth of Saccharomyces cerevisiae." Microbiology 155, no. 12 (2009): 3827–37. http://dx.doi.org/10.1099/mic.0.030213-0.

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Glucose repression of the tricarboxylic acid (TCA) cycle in Saccharomyces cerevisiae was investigated under different environmental conditions using 13C-tracer experiments. Real-time quantification of the volatile metabolites ethanol and CO2 allowed accurate carbon balancing. In all experiments with the wild-type, a strong correlation between the rates of growth and glucose uptake was observed, indicating a constant yield of biomass. In contrast, glycerol and acetate production rates were less dependent on the rate of glucose uptake, but were affected by environmental conditions. The glycerol

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13

Wu, Meiling, Hongxing Li, Shan Wei, et al. "Simulating Extracellular Glucose Signals Enhances Xylose Metabolism in Recombinant Saccharomyces cerevisiae." Microorganisms 8, no. 1 (2020): 100. http://dx.doi.org/10.3390/microorganisms8010100.

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Efficient utilization of both glucose and xylose from lignocellulosic biomass would be economically beneficial for biofuel production. Recombinant Saccharomyces cerevisiae strains with essential genes and metabolic networks for xylose metabolism can ferment xylose; however, the efficiency of xylose fermentation is much lower than that of glucose, the preferred carbon source of yeast. Implications from our previous work suggest that activation of the glucose sensing system may benefit xylose metabolism. Here, we show that deleting cAMP phosphodiesterase genes PDE1 and PDE2 increased PKA activit

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14

van Oevelen, Chris J. C., Hetty A. A. M. van Teeffelen, and H. T. Marc Timmers. "Differential Requirement of SAGA Subunits for Mot1p and Taf1p Recruitment in Gene Activation." Molecular and Cellular Biology 25, no. 12 (2005): 4863–72. http://dx.doi.org/10.1128/mcb.25.12.4863-4872.2005.

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ABSTRACT Transcription activation in yeast (Saccharomyces cerevisiae) involves ordered recruitment of transcription factor complexes, such as TFIID, SAGA, and Mot1p. Previously, we showed that both Mot1p and Taf1p are recruited to the HXT2 and HXT4 genes, which encode hexose transporter proteins. Here, we show that SAGA also binds to the HXT2 and HXT4 promoters and plays a pivotal role in the recruitment of Mot1p and Taf1p. The deletion of either SPT3 or SPT8 reduces Mot1p binding to HXT2 and HXT4. Surprisingly, the deletion of GCN5 reduces Taf1p binding to both promoters. When GCN5 is deleted

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15

Zheng, Liyuan, Shan Wei, Meiling Wu, et al. "Improving Xylose Fermentation in Saccharomyces cerevisiae by Expressing Nuclear-Localized Hexokinase 2." Microorganisms 8, no. 6 (2020): 856. http://dx.doi.org/10.3390/microorganisms8060856.

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Understanding the relationship between xylose and the metabolic regulatory systems is a prerequisite to enhance xylose utilization in recombinant S. cerevisiae strains. Hexokinase 2 (Hxk2p) is an intracellular glucose sensor that localizes to the cytoplasm or the nucleus depending on the carbon source. Hxk2p interacts with Mig1p to regulate gene transcription in the nucleus. Here, we investigated the effect of nucleus-localized Hxk2p and Mig1p on xylose fermentation. The results show that the expression of HXK2S14A, which encodes a constitutively nucleus-localized Hxk2p, increased the xylose c

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16

Guo, Yan Bin, Jinyun Li, Lei Li, et al. "Mutations That Disrupt Either the pqq or the gdh Gene of Rahnella aquatilis Abolish the Production of an Antibacterial Substance and Result in Reduced Biological Control of Grapevine Crown Gall." Applied and Environmental Microbiology 75, no. 21 (2009): 6792–803. http://dx.doi.org/10.1128/aem.00902-09.

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ABSTRACT Rahnella aquatilis HX2, a biocontrol agent for grapevine crown gall caused by Agrobacterium vitis, produces an antibacterial substance that inhibits the growth of A. vitis in vitro. In this study, we show that MH15 and MH16, two Tn5-induced mutants of HX2, have lost their abilities to inhibit A. vitis and have reduced biocontrol activities; they grow in logarithmic phase at a rate similar to that of the wild type and have single Tn5 insertions. They are also impaired in producing pyrroloquinoline quinone (PQQ) or glucose dehydrogenase (GDH). Complementation of MH15 and MH16 with cosmi

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17

Hirai, Kenshi, Takuya Idemoto, Shiho Kato, Akihiko Ichiishi, Fumiyasu Fukumori, and Makoto Fujimura. "Deletion of the col-26 Transcription Factor Gene and a Point Mutation in the exo-1 F-Box Protein Gene Confer Sorbose Resistance in Neurospora crassa." Journal of Fungi 8, no. 11 (2022): 1169. http://dx.doi.org/10.3390/jof8111169.

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L-Sorbose induces hyperbranching of hyphae, which results in colonial growth in Neurospora crassa. The sor-4 gene, which encodes a glucose sensor that acts in carbon catabolite repression (CCR), has been identified as a sorbose resistance gene. In this study, we found that the deletion mutant of col-26, which encodes an AmyR-like transcription factor that acts in CCR, displayed sorbose resistance. In contrast, the deletion mutants of other CCR genes, such as a hexokinase (hxk-2), an AMP-activated S/T protein kinase (prk-10), and a transcription factor (cre-1), showed no sorbose resistance. Dou

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18

Dashtban, Mehdi, Xin Wen, Paramjit K. Bajwa, Chi-Yip Ho, and Hung Lee. "Deletion of hxk1 gene results in derepression of xylose utilization in Scheffersomyces stipitis." Journal of Industrial Microbiology & Biotechnology 42, no. 6 (2015): 889–96. http://dx.doi.org/10.1007/s10295-015-1614-9.

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Zhang, Qiuyu, Li Xu, Sheng Yuan, et al. "NGT1 Is Essential for N-Acetylglucosamine-Mediated Filamentous Growth Inhibition and HXK1 Functions as a Positive Regulator of Filamentous Growth in Candida tropicalis." International Journal of Molecular Sciences 21, no. 11 (2020): 4036. http://dx.doi.org/10.3390/ijms21114036.

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Candida tropicalis is a pathogenic fungus that can cause opportunistic infections in humans. The ability of Candida species to transition between yeast and filamentous growth forms is essential to their ability to undergo environmental adaptation and to maintain virulence. In other fungal species, such as Candida albicans, N-acetylglucosamine (GlcNAc) can induce filamentous growth, whereas it suppresses such growth in C. tropicalis. In the present study, we found that knocking out the GlcNA-specific transporter gene NGT1 was sufficient to enhance C. tropicalis filamentous growth on Lee’s plus

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20

Chen, Steve S. L., Sheau-Fen Lee, Chin-Kai Chuang, and V. Samuel Raj. "trans-Dominant Interference with Human Immunodeficiency Virus Type 1 Replication and Transmission in CD4+ Cells by an Envelope Double Mutant." Journal of Virology 73, no. 10 (1999): 8290–302. http://dx.doi.org/10.1128/jvi.73.10.8290-8302.1999.

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ABSTRACT We previously reported that a human immunodeficiency virus type 1 (HIV-1) envelope (Env) mutant with the whole cytoplasmic domain deleted, denoted mutant TC, is able to dominantly interfere with wild-type (wt) virus infectivity. In the present study, the feasibility of developing a dominant negative mutant-based genetic anti-HIV strategy targeting the gp41 cytoplasmic domain was investigated. Mutants TC and 427,TC, a TC derivative with a Trp-to-Ser substitution introduced into residue 427 in the CD4-binding site, and a series of mutants with deletions in the cytoplasmic domain, effect

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Kim, Daehee, Ji-Yoon Song, and Ji-Sook Hahn. "Improvement of Glucose Uptake Rate and Production of Target Chemicals by Overexpressing Hexose Transporters and Transcriptional Activator Gcr1 in Saccharomyces cerevisiae." Applied and Environmental Microbiology 81, no. 24 (2015): 8392–401. http://dx.doi.org/10.1128/aem.02056-15.

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ABSTRACTMetabolic engineering to increase the glucose uptake rate might be beneficial to improve microbial production of various fuels and chemicals. In this study, we enhanced the glucose uptake rate inSaccharomyces cerevisiaeby overexpressing hexose transporters (HXTs). Among the 5 tested HXTs (Hxt1, Hxt2, Hxt3, Hxt4, and Hxt7), overexpression of high-affinity transporter Hxt7 was the most effective in increasing the glucose uptake rate, followed by moderate-affinity transporters Hxt2 and Hxt4. Deletion ofSTD1andMTH1, encoding corepressors ofHXTgenes, exerted differential effects on the gluc

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22

Kim, Hun, Jonathon E. Smith, John B. Ridenour, Charles P. Woloshuk, and Burton H. Bluhm. "HXK1 regulates carbon catabolism, sporulation, fumonisin B1 production and pathogenesis in Fusarium verticillioides." Microbiology 157, no. 9 (2011): 2658–69. http://dx.doi.org/10.1099/mic.0.052506-0.

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In Fusarium verticillioides, a ubiquitous pathogen of maize, virulence and mycotoxigenesis are regulated in response to the types and amounts of carbohydrates present in maize kernels. In this study, we investigated the role of a putative hexokinase-encoding gene (HXK1) in growth, development and pathogenesis. A deletion mutant (Δhxk1) of HXK1 was not able to grow when supplied with fructose as the sole carbon source, and growth was impaired when glucose, sucrose or maltotriose was provided. Additionally, the Δhxk1 mutant produced unusual swollen hyphae when provided with fructose, but not glu

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23

Kang, Sang-Moo, and Casey D. Morrow. "Genetic Analysis of a Unique Human Immunodeficiency Virus Type 1 (HIV-1) with a Primer Binding Site Complementary to tRNAMet Supports a Role for U5-PBS Stem-Loop RNA Structures in Initiation of HIV-1 Reverse Transcription." Journal of Virology 73, no. 3 (1999): 1818–27. http://dx.doi.org/10.1128/jvi.73.3.1818-1827.1999.

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ABSTRACT Human immunodeficiency virus type 1 (HIV-1) exclusively uses tRNA3 Lys to initiate reverse transcription. A novel HIV-1 mutant which stably utilizes tRNAMet rather than tRNA3 Lys as a primer was previously identified [HXB2(Met-AC] (S.-M. Kang, Z. Zhang, and C. D. Morrow, J. Virol. 71:207–217, 1997). Comparison of RNA secondary structures of the unique sequence (U5)-primer binding site (PBS) viral RNA genome alone or complexed with tRNAMet of HXB2(Met-AC) revealed structural motifs in common with the U5-PBS of the wild-type virus. In the current study, mutations were constructed to alt

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Salzwedel, Karl, John T. West, and Eric Hunter. "A Conserved Tryptophan-Rich Motif in the Membrane-Proximal Region of the Human Immunodeficiency Virus Type 1 gp41 Ectodomain Is Important for Env-Mediated Fusion and Virus Infectivity." Journal of Virology 73, no. 3 (1999): 2469–80. http://dx.doi.org/10.1128/jvi.73.3.2469-2480.1999.

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ABSTRACT Mutations were introduced into the ectodomain of the human immunodeficiency virus type 1 (HIV-1) transmembrane envelope glycoprotein, gp41, within a region immediately adjacent to the membrane-spanning domain. This region, which is predicted to form an α-helix, contains highly conserved hydrophobic residues and is unusually rich in tryptophan residues. In addition, this domain overlaps the epitope of a neutralizing monoclonal antibody, 2F5, as well as the sequence corresponding to a peptide, DP-178, shown to potently neutralize virus. Site-directed mutagenesis was used to create delet

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Zekhnini, Abdelghani, Marcel Albacar, Antonio Casamayor, and Joaquín Ariño. "The ENA1 Na+-ATPase Gene Is Regulated by the SPS Sensing Pathway and the Stp1/Stp2 Transcription Factors." International Journal of Molecular Sciences 24, no. 6 (2023): 5548. http://dx.doi.org/10.3390/ijms24065548.

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The Saccharomyces cerevisiae ENA1 gene, encoding a Na+-ATPase, responds transcriptionally to the alkalinization of the medium by means of a network of signals that involves the Rim101, the Snf1 and PKA kinases, and the calcineurin/Crz1 pathways. We show here that the ENA1 promoter also contains a consensus sequence, located at nt −553/−544, for the Stp1/2 transcription factors, the downstream components of the amino acid sensing SPS pathway. Mutation of this sequence or deletion of either STP1 or STP2 decreases the activity of a reporter containing this region in response to alkalinization as

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Lascaris, Romeo, Jan Piwowarski, Hans van der Spek, Joost Teixeira de Mattos, Les Grivell, and Jolanda Blom. "Overexpression of HAP4 in glucose-derepressed yeast cells reveals respiratory control of glucose-regulated genes." Microbiology 150, no. 4 (2004): 929–34. http://dx.doi.org/10.1099/mic.0.26742-0.

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A link between control of respiration and glucose repression in yeast is reported. The HAP4 gene was overexpressed in a Δmig1 deletion background, generating a mutant in which respiratory function is stimulated and glucose repression is diminished. Although this combination does not result in derepression of genes encoding proteins involved in respiratory function, it nevertheless generates resistance against 2-deoxyglucose and hence contributes to more derepressed growth characteristics. Unexpectedly, overexpression of HAP4 in the Δmig1 deletion strain causes strong repression of several targ

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Fleck, Christian B., and Matthias Brock. "Aspergillus fumigatus Catalytic Glucokinase and Hexokinase: Expression Analysis and Importance for Germination, Growth, and Conidiation." Eukaryotic Cell 9, no. 7 (2010): 1120–35. http://dx.doi.org/10.1128/ec.00362-09.

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ABSTRACT Fungi contain several hexokinases, which are involved either in sugar phosphorylation or in carbon source sensing. Glucose and fructose phosphorylations appear to rely exclusively on glucokinase and hexokinase. Here, we characterized the catalytic glucokinase and hexokinase from the opportunistic human pathogen Aspergillus fumigatus and showed that both enzymes display different biochemical properties and play different roles during growth and development. Glucokinase efficiently activates glucose and mannose but activates fructose only to a minor extent. Hexokinase showed a high effi

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Flick, J. S., and M. Johnston. "Two systems of glucose repression of the GAL1 promoter in Saccharomyces cerevisiae." Molecular and Cellular Biology 10, no. 9 (1990): 4757–69. http://dx.doi.org/10.1128/mcb.10.9.4757-4769.1990.

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Expression of the GAL1 gene in Saccharomyces cerevisiae is strongly repressed by growth on glucose. We show that two sites within the GAL1 promoter mediate glucose repression. First, glucose inhibits transcription activation by GAL4 protein through UASG. Second, a promoter element, termed URSG, confers glucose repression independently of GAL4. We have localized the URSG sequences responsible for glucose repression to an 87-base-pair fragment located between UASG and the TATA box. Promoters deleted for small (20-base-pair) segments that span this sequence are still subject to glucose repression

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Flick, J. S., and M. Johnston. "Two systems of glucose repression of the GAL1 promoter in Saccharomyces cerevisiae." Molecular and Cellular Biology 10, no. 9 (1990): 4757–69. http://dx.doi.org/10.1128/mcb.10.9.4757.

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Expression of the GAL1 gene in Saccharomyces cerevisiae is strongly repressed by growth on glucose. We show that two sites within the GAL1 promoter mediate glucose repression. First, glucose inhibits transcription activation by GAL4 protein through UASG. Second, a promoter element, termed URSG, confers glucose repression independently of GAL4. We have localized the URSG sequences responsible for glucose repression to an 87-base-pair fragment located between UASG and the TATA box. Promoters deleted for small (20-base-pair) segments that span this sequence are still subject to glucose repression

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Lewis, D. A., and L. F. Bisson. "The HXT1 gene product of Saccharomyces cerevisiae is a new member of the family of hexose transporters." Molecular and Cellular Biology 11, no. 7 (1991): 3804–13. http://dx.doi.org/10.1128/mcb.11.7.3804-3813.1991.

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Two novel genes affecting hexose transport in the yeast Saccharomyces cerevisiae have been identified. The gene HXT1 (hexose transport), isolated from plasmid pSC7, was sequenced and found to encode a hydrophobic protein which is highly homologous to the large family of sugar transporter proteins from eucaryotes and procaryotes. Multicopy expression of the HXT1 gene restored high-affinity glucose transport to the snf3 mutant, which is deficient in a significant proportion of high-affinity glucose transport. HXT1 was unable to complement the snf3 growth defect in low copy number. The HXT1 prote

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Lewis, D. A., and L. F. Bisson. "The HXT1 gene product of Saccharomyces cerevisiae is a new member of the family of hexose transporters." Molecular and Cellular Biology 11, no. 7 (1991): 3804–13. http://dx.doi.org/10.1128/mcb.11.7.3804.

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Two novel genes affecting hexose transport in the yeast Saccharomyces cerevisiae have been identified. The gene HXT1 (hexose transport), isolated from plasmid pSC7, was sequenced and found to encode a hydrophobic protein which is highly homologous to the large family of sugar transporter proteins from eucaryotes and procaryotes. Multicopy expression of the HXT1 gene restored high-affinity glucose transport to the snf3 mutant, which is deficient in a significant proportion of high-affinity glucose transport. HXT1 was unable to complement the snf3 growth defect in low copy number. The HXT1 prote

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Karri, Srinivasu, Quinn Dickinson, Jing Jia, et al. "The role of hexokinases in epigenetic regulation: altered hexokinase expression and chromatin stability in yeast." Epigenetics & Chromatin 17, no. 1 (2024). http://dx.doi.org/10.1186/s13072-024-00551-9.

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Abstract Background Human hexokinase 2 (HK2) plays an important role in regulating Warburg effect, which metabolizes glucose to lactate acid even in the presence of ample oxygen and provides intermediate metabolites to support cancer cell proliferation and tumor growth. HK2 overexpression has been observed in various types of cancers and targeting HK2-driven Warburg effect has been suggested as a potential cancer therapeutic strategy. Given that epigenetic enzymes utilize metabolic intermediates as substrates or co-factors to carry out post-translational modification of histones and nucleic ac

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Müller, Henry, Antoine Lesur, Gunnar Dittmar, Marc Gentzel, and Karina Kettner. "Proteomic consequences of TDA1 deficiency in Saccharomyces cerevisiae: Protein kinase Tda1 is essential for Hxk1 and Hxk2 serine 15 phosphorylation." Scientific Reports 12, no. 1 (2022). http://dx.doi.org/10.1038/s41598-022-21414-x.

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AbstractHexokinase 2 (Hxk2) of Saccharomyces cerevisiae is a dual function hexokinase, acting as a glycolytic enzyme and being involved in the transcriptional regulation of glucose-repressible genes. Relief from glucose repression is accompanied by phosphorylation of Hxk2 at serine 15, which has been attributed to the protein kinase Tda1. To explore the role of Tda1 beyond Hxk2 phosphorylation, the proteomic consequences of TDA1 deficiency were investigated by difference gel electrophoresis (2D-DIGE) comparing a wild type and a Δtda1 deletion mutant. To additionally address possible consequenc

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Valiakhmetov, Airat. "Suppression of glycolysis decreases sugar-induced cell death in Saccharomyces cerevisiae." FEMS Microbiology Letters, February 14, 2025. https://doi.org/10.1093/femsle/fnaf026.

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Abstract Although 30 years have passed since the description of sugar-induced cell death (SICD), the specific molecular mechanism that triggers this process remains unclear. This paper attempts to shed light on the relationship between SICD and glucose catabolism. In yeast cells, glucose is involved not only in energy-producing processes but also in the synthesis of reserve hydrocarbons. It is known that disruption of trehalose synthesis leads to significant changes in the physiology of S. cerevisiae. The present study shows that deletion of the TPS1 gene resulted in a 44% suppression of SICD

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Nonaka, Kazuki, Kohei Nishimura, Kazuma Uesaka та ін. "Snf1 and yeast GSK3-β activates Tda1 to suppress glucose starvation signaling". EMBO Reports, 24 квітня 2025. https://doi.org/10.1038/s44319-025-00456-y.

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Abstract In budding yeast, the presence of glucose, a preferred energy source, suppresses the expression of respiration-related genes through a process known as glucose repression. Conversely, under glucose starvation conditions, Snf1 phosphorylates and activates downstream factors, relieving this repression and allowing cells to adapt. Recently, the Tda1 protein kinase has been implicated in these glucose starvation responses, although its function remains largely uncharacterized. In this study, we demonstrate that Snf1 and yeast glycogen synthase kinase 3-beta (GSK3-β) independently phosphor

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Nijland, Jeroen G., Hyun Yong Shin, Eleonora Dore, Donny Rudinatha, Paul P. de Waal, and Arnold J. M. Driessen. "D-glucose overflow metabolism in an evolutionary engineered high-performance D-xylose consuming Saccharomyces cerevisiae strain." FEMS Yeast Research, November 24, 2020. http://dx.doi.org/10.1093/femsyr/foaa062.

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Abstract Co-consumption of D-xylose and D-glucose by Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. There is a need for improved sugar conversion rates to minimize fermentation times. Previously, we have employed evolutionary engineering to enhance D-xylose transport and metabolism in the presence of D-glucose in a xylose-fermenting S. cerevisiae strain devoid of hexokinases. Re-introduction of Hxk2 in the high performance xylose-consuming strains restored D-glucose utilization during D-xylose/D-glucose co-metabolism, but at rates lower than the non-

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Jin, Chaeyeon, Sojeong Kim, Seokjun Moon, Hyunbin Jin, and Ji-Sook Hahn. "Efficient production of shinorine, a natural sunscreen material, from glucose and xylose by deleting HXK2 encoding hexokinase in Saccharomyces cerevisiae." FEMS Yeast Research 21, no. 7 (2021). http://dx.doi.org/10.1093/femsyr/foab053.

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ABSTRACT Mycosporine-like amino acids (MAAs), microbial secondary metabolites with ultraviolet (UV) absorption properties, are promising natural sunscreen materials. Due to the low efficiency of extracting MAAs from natural producers, production in heterologous hosts has recently received attention. Shinorine is a well characterized MAA with strong UV-A absorption property. Previous, we developed Saccharomyces cerevisiae strain producing shinorine by introducing four shinorine biosynthetic genes from cyanobacterium Nostoc punctiforme. Shinorine is produced from sedoheptulose 7-phosphate (S7P),

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Qin, Hong. "Estimating network changes from lifespan measurements using a parsimonious gene network model of cellular aging." BMC Bioinformatics 20, no. 1 (2019). http://dx.doi.org/10.1186/s12859-019-3177-7.

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Abstract Background Cellular aging is best studied in the budding yeast Saccharomyces cerevisiae. As an example of a pleiotropic trait, yeast lifespan is influenced by hundreds of interconnected genes. However, no quantitative methods are currently available to infer system-level changes in gene networks during cellular aging. Results We propose a parsimonious mathematical model of cellular aging based on stochastic gene interaction networks. This network model is made of only non-aging components: the strength of gene interactions declines with a constant mortality rate. Death of a cell occur

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Xu, Qiaolin, Shanshan Gao, Sasa Zhang, Kui Li, and Yanbin Guo. "Disruption of the cell division protein ftsK gene changes elemental selenium generation, selenite tolerance, and cell morphology in Rahnella aquatilis HX2." Journal of Applied Microbiology, June 13, 2024. http://dx.doi.org/10.1093/jambio/lxae142.

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Abstract Aims Some studies have indicated that the alterations in cellular morphology induced by selenite [Se(Ⅳ)] may be attributed to its inhibitory effects on cell division. However, whether the genes associated with cell division are implicated in Se(Ⅳ) metabolism remains unclear. Methods and results The ftsK gene in Rahnella aquatilis HX2 was mutated with an in-frame deletion strategy. The ftsK mutation strongly reduced the tolerance to selenite [Se(Ⅳ)] and the production of red elemental selenium [Se(0)] in R. aquatilis HX2, and this effect could not be attributed solely to the inhibition

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Jing, Peng, Zhongnan Xu, Lei Li, Bingjie Zhao, and Yanbin Guo. "Disruption of the Sensor Kinase phoQ Gene Decreases Acid Resistance in Plant Growth-promoting Rhizobacterium Rahnella aquatilis HX2." Journal of Applied Microbiology, January 18, 2023. http://dx.doi.org/10.1093/jambio/lxad009.

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Abstract Aims Rahnella aquatilis HX2, a promising plant growth-promoting rhizobacterium (PGPR) in the field, contains genes homologous to the PhoP/PhoQ two-component regulatory system. Although this system regulates stress response in numerous pathogens, PhoP/PhoQ characterization in a PGPR has not received in-depth exploration. Methods and Results The phoQ gene was mutated in strain HX2 using an in-frame deletion strategy. Compared to the wild type, the phoQ mutant exhibited increased sensitivity to acidic conditions (pH 4.0) in a chemically defined medium and in mild acidic natural soil (pH

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Agrawal, Parul, Maria L. Knudsen, Anna MacCamy, et al. "Short CDRL1 in intermediate VRC01-like mAbs is not sufficient to overcome key glycan barriers on HIV-1 Env." Journal of Virology, September 6, 2024. http://dx.doi.org/10.1128/jvi.00744-24.

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ABSTRACT VRC01-class broadly neutralizing antibodies (bnAbs) have been isolated from people with HIV-1, but they have not yet been elicited by vaccination. They are extensively somatically mutated and sometimes accumulate CDRL1 deletions. Such indels may allow VRC01-class antibodies to accommodate the glycans expressed on a conserved N276 N-linked glycosylation site in loop D of the gp120 subunit. These glycans constitute a major obstacle in the development of VRC01-class antibodies, as unmutated antibody forms are unable to accommodate them. Although immunizations of knock-in mice expressing

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Nijland, Jeroen G., Xiang Li, Hyun Yong Shin, Paul P. de Waal, and Arnold J. M. Driessen. "Efficient, D-glucose insensitive, growth on D-xylose by an evolutionary engineered Saccharomyces cerevisiae strain." FEMS Yeast Research 19, no. 8 (2019). http://dx.doi.org/10.1093/femsyr/foz083.

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ABSTRACT Optimizing D-xylose consumption in Saccharomyces cerevisiae is essential for cost-efficient cellulosic bioethanol production. An evolutionary engineering approach was used to elevate D-xylose consumption in a xylose-fermenting S. cerevisiae strain carrying the D-xylose-specific N367I mutation in the endogenous chimeric Hxt36 hexose transporter. This strain carries a quadruple hexokinase deletion that prevents glucose utilization, and allows for selection of improved growth rates on D-xylose in the presence of high D-glucose concentrations. Evolutionary engineering resulted in D-glucos

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Wang, Ling, Qing Liu, Shuailing Ge, et al. "Genomic footprints related with adaptation and fumonisins production in Fusarium proliferatum." Frontiers in Microbiology 13 (September 21, 2022). http://dx.doi.org/10.3389/fmicb.2022.1004454.

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Fusarium proliferatum is the principal etiological agent of rice spikelet rot disease (RSRD) in China, causing yield losses and fumonisins contamination in rice. The intraspecific variability and evolution pattern of the pathogen is poorly understood. Here, we performed whole-genome resequencing of 67 F. proliferatum strains collected from major rice-growing regions in China. Population structure indicated that eastern population of F. proliferatum located in Yangtze River with the high genetic diversity and recombinant mode that was predicted as the putative center of origin. Southern populat

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Van Leemputte, Frederik, Ward Vanthienen, Stefanie Wijnants, Griet Van Zeebroeck та Johan M. Thevelein. "Aberrant Intracellular pH Regulation Limiting Glyceraldehyde-3-Phosphate Dehydrogenase Activity in the Glucose-Sensitive Yeast tps1Δ Mutant". mBio 11, № 5 (2020). http://dx.doi.org/10.1128/mbio.02199-20.

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ABSTRACT Whereas the yeast Saccharomyces cerevisiae shows great preference for glucose as a carbon source, a deletion mutant in trehalose-6-phosphate synthase, tps1Δ, is highly sensitive to even a few millimolar glucose, which triggers apoptosis and cell death. Glucose addition to tps1Δ cells causes deregulation of glycolysis with hyperaccumulation of metabolites upstream and depletion downstream of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The apparent metabolic barrier at the level of GAPDH has been difficult to explain. We show that GAPDH isozyme deletion, especially Tdh3, further a

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Zheng, Caijuan, Shuxin Hou, Yu Zhou, Changyuan Yu, and Hao Li. "Regulation of the PFK1 gene on the interspecies microbial competition behavior of Saccharomyces cerevisiae." Applied Microbiology and Biotechnology 108, no. 1 (2024). http://dx.doi.org/10.1007/s00253-024-13091-9.

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Abstract Saccharomyces cerevisiae is a widely used strain for ethanol fermentation; meanwhile, efficient utilization of glucose could effectively promote ethanol production. The PFK1 gene is a key gene for intracellular glucose metabolism in S. cerevisiae. Our previous work suggested that although deletion of the PFK1 gene could confer higher oxidative tolerance to S. cerevisiae cells, the PFK1Δ strain was prone to contamination by other microorganisms. High interspecies microbial competition ability is vital for the growth and survival of microorganisms in co-cultures. The result of our previ

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