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Artykuły w czasopismach na temat „I-Motifs”

Autor: Grafiati

Data publikacji: 25 stycznia 2025

Data aktualizacji: 31 lipca 2025

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1

Shamim, Amen, Maria Razzaq, and Kyeong Kyu Kim. "MD-TSPC4: Computational Method for Predicting the Thermal Stability of I-Motif." International Journal of Molecular Sciences 22, no. 1 (2020): 61. http://dx.doi.org/10.3390/ijms22010061.

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I-Motif is a tetrameric cytosine-rich DNA structure with hemi-protonated cytosine: cytosine base pairs. Recent evidence showed that i-motif structures in human cells play regulatory roles in the genome. Therefore, characterization of novel i-motifs and investigation of their functional implication are urgently needed for comprehensive understanding of their roles in gene regulation. However, considering the complications of experimental investigation of i-motifs and the large number of putative i-motifs in the genome, development of an in silico tool for the characterization of i-motifs in the

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2

Teklasani, Juita, and Luh Putu Tejawati Ni. "MAKNA FILOSOFI MOTIF KAIN TENUN SONGKE DI DESA PONNG LENGOR KECAMATAN RAHONG UTARA KABUPATEN MANGGARAI NUSA TENGGARA TIMUR." Widyadari 23, no. 2 (2022): 389–99. https://doi.org/10.5281/zenodo.7190282.

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<em>This study aims to determine (1) the forms of Songke Manggarai woven fabric motifs; (2) Factors influencing the development of Songke Manggarai woven fabric motifs; (3) The philosophical meaning of Songke woven fabric motifs. The theory used in this research is symbol theory, consumer culture theory and semiotic theory. In an effort to obtain data, the method of determining informants, data collection methods, and data processing methods is used. This study concludes that (1) the Songke Manggarai woven motifs include the wela runu motif, the ranggong motif, the wela kaweng motif, the ntala

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Catur, Edi Widodo. "THE USE OF FRACTAL GEOMETRY IN TILING MOTIF DESIGN." International Journal of Innovative Research in Advanced Engineering VII, no. Iv (2020): 263–66. https://doi.org/10.26562/ijirae.2020.v0704.001.

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In this paper I will present the use of fractal geometry to design tile motifs. A fractal is a geometric figure that combines the several characteristics among others: its parts have the same form as the whole, fragmented, and formation by iteration. The concept of fractals has been spread over all fields of sciences, technology, and art. This paper aims to provide an algorithm to creating motifs of tile algorithm for create the tile motif consists of base, iteration, coloration and duplication. In order to help the reader better understand the algorithm, I will present some script using Matla

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4

Jiang, Guimei, Lijun Xu, Kewei Wang, et al. "Quinaldine red as a fluorescent light-up probe for i-motif structures." Analytical Methods 9, no. 10 (2017): 1585–88. http://dx.doi.org/10.1039/c7ay00301c.

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We report a new fluorescent probe for i-motif structures. When binding to i-motifs, quinaldine red exhibits a remarkable increase in its fluorescence, which benefits i-motif-involved label-free systems.

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5

Ruggiero, Emanuela, Sara Lago, Primož Šket, et al. "A dynamic i-motif with a duplex stem-loop in the long terminal repeat promoter of the HIV-1 proviral genome modulates viral transcription." Nucleic Acids Research 47, no. 21 (2019): 11057–68. http://dx.doi.org/10.1093/nar/gkz937.

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Abstract I-motifs are non-canonical nucleic acids structures characterized by intercalated H-bonds between hemi-protonated cytosines. Evidence on the involvement of i-motif structures in the regulation of cellular processes in human cells has been consistently growing in the recent years. However, i-motifs within non-human genomes have never been investigated. Here, we report the characterization of i-motifs within the long terminal repeat (LTR) promoter of the HIV-1 proviral genome. Biophysical and biochemical analysis revealed formation of a predominant i-motif with an unprecedented loop com

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6

Martins, Alexandra, Christian H. Gross, and Stewart Shuman. "Mutational Analysis of Vaccinia Virus Nucleoside Triphosphate Phosphohydrolase I, a DNA-Dependent ATPase of the DExH Box Family." Journal of Virology 73, no. 2 (1999): 1302–8. http://dx.doi.org/10.1128/jvi.73.2.1302-1308.1999.

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ABSTRACT Vaccinia virus nucleoside triphosphate phosphohydrolase I (NPH-I) is a DNA-dependent ATPase that serves as a transcription termination factor during viral mRNA synthesis. NPH-I is a member of the DExH box family of nucleic acid-dependent nucleoside triphosphatases (NTPases), which is defined by the presence of several conserved sequence motifs. We have assessed the contributions of individual amino acids (underlined) in motifs I (GxGKT), II (DExHN), III (SAT), and VI (QxxGRxxR) to ATP hydrolysis by performing alanine scanning mutagenesis. Significant decrements in ATPase activity resu

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7

Nufer, Oliver, Svend Guldbrandsen, Martin Degen, et al. "Role of cytoplasmic C-terminal amino acids of membrane proteins in ER export." Journal of Cell Science 115, no. 3 (2002): 619–28. http://dx.doi.org/10.1242/jcs.115.3.619.

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Export of membrane proteins from the ER is believed to be selective and require transport signals, but the identity of such signals has remained elusive. The recycling type I membrane protein ERGIC-53 carries a C-terminal diphenylalanine motif that is required for efficient ER export. Here we show that this motif can be functionally substituted by a single phenylalanine or tyrosine at position -2, two leucines or isoleucines at position -1 and -2 or a single valine at position -1. These motifs are common among mammalian type I membrane proteins. A single C-terminal valine, but none of the othe

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8

Saha, Puja, Deepanjan Panda, Diana Müller, Arunabha Maity, Harald Schwalbe, and Jyotirmayee Dash. "In situ formation of transcriptional modulators using non-canonical DNA i-motifs." Chemical Science 11, no. 8 (2020): 2058–67. http://dx.doi.org/10.1039/d0sc00514b.

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Grote, Johanna, Beate Neumann, Hans-Georg Stammler, and Norbert W. Mitzel. "Diversity of aggregation motifs in gold(i) dithiocarboxylate complexes." Dalton Transactions 47, no. 13 (2018): 4701–6. http://dx.doi.org/10.1039/c8dt00342d.

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Molecular structures and aggregation motifs of different substituted gold(i) dithiocarboxylate complexes are presented and a correlation between the substituent and the aggregation motif is discussed.

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10

Grote, Johanna, Beate Neumann, Hans-Georg Stammler, and Norbert W. Mitzel. "Silver(i) dithiocarboxylate complexes – clustering and aggregation." Dalton Transactions 47, no. 17 (2018): 6036–40. http://dx.doi.org/10.1039/c8dt00279g.

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Molecular structures and aggregation motifs of a series of neutral homoleptic silver(i) dithiocarboxylate are presented and a correlation between the substituent and aggregation motif as well as the differences from the (almost) analogous gold(i) complexes are discussed.

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11

Yanti Pantur, Mariani Irma, and Ika Ismurdiyahwati. "Songke Fabric in Libo, Wela Kaweng and Su'i Motif from Perak Village, Manggarai Regency, East Nusa Tenggara, in Study of Visual Aesthetics of Meaning and Function." IMAGIONARY 1, no. 2 (2023): 76–82. http://dx.doi.org/10.51353/jim.v1i2.753.

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Songke fabrics with libo, wela kaweng and su'I motifs are a type of Indonesian textile work. Songke cloth with libo, wela kaweng and su'I motifs is a textile work from Perak village, Manggarai Regency, East Nusa Tenggara. This research takes the topic of the libo, wela kaweng and suâ€™I motifs on songke cloth in Perak village, Manggarai Regency, because these motifs are superior motifs that are often produced and a cultural heritage that has its own meaning and function. Therefore, this study aims to determine the visual aesthetic meaning and function of songke cloth in libo, wela kaweng and s

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12

Wright, Elisé P., Mahmoud A. S. Abdelhamid, Michelle O. Ehiabor, et al. "Epigenetic modification of cytosines fine tunes the stability of i-motif DNA." Nucleic Acids Research 48, no. 1 (2019): 55–62. http://dx.doi.org/10.1093/nar/gkz1082.

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Abstract i-Motifs are widely used in nanotechnology, play a part in gene regulation and have been detected in human nuclei. As these structures are composed of cytosine, they are potential sites for epigenetic modification. In addition to 5-methyl- and 5-hydroxymethylcytosine modifications, recent evidence has suggested biological roles for 5-formylcytosine and 5-carboxylcytosine. Herein the human telomeric i-motif sequence was used to examine how these four epigenetic modifications alter the thermal and pH stability of i-motifs. Changes in melting temperature and transitional pH depended on b

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13

Leporcq, Clémentine, Yannick Spill, Delphine Balaramane, Christophe Toussaint, Michaël Weber, and Anaïs Flore Bardet. "TFmotifView: a webserver for the visualization of transcription factor motifs in genomic regions." Nucleic Acids Research 48, W1 (2020): W208—W217. http://dx.doi.org/10.1093/nar/gkaa252.

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Abstract Transcription factors (TFs) regulate the expression of gene expression. The binding specificities of many TFs have been deciphered and summarized as position-weight matrices, also called TF motifs. Despite the availability of hundreds of known TF motifs in databases, it remains non-trivial to quickly query and visualize the enrichment of known TF motifs in genomic regions of interest. Towards this goal, we developed TFmotifView, a web server that allows to study the distribution of known TF motifs in genomic regions. Based on input genomic regions and selected TF motifs, TFmotifView p

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14

HAMAHATA, Akiko, Yoshimi TAKATA, Tomoharu GOMI, and Motoji FUJIOKA. "Probing the S-adenosylmethionine-binding site of rat guanidinoacetate methyltransferase. Effect of site-directed mutagenesis of residues that are conserved across mammalian non-nucleic acid methyltransferases. Effect of site-directed mutagenesis of residues that are conserved across mammalian non-nucleic acid methyltransferases." Biochemical Journal 317, no. 1 (1996): 141–45. http://dx.doi.org/10.1042/bj3170141.

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Most mammalian non-nucleic acid methyltransferases share three sequence motifs. To gain insight into the S-adenosylmethionine (AdoMet)-binding site of guanidinoacetate methyltransferase, we mutated several conserved residues that are found in or near motifs I and II. Conversion of either of two glycine residues of motif I (Gly67 and Gly69) to an alanine resulted in an inactive enzyme. These enzymes, although having UV absorption, fluorescence and far-UV CD spectra virtually identical with those of the wild-type enzyme, seem to be conformationally different from the wild-type enzyme as judged b

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15

Kisbali, Tamás Péter. "“LUDUS NATURAE”: SHELLS AS SCULPTED MOTIFS IN ANCIENT GREEK ART." RSUH/RGGU Bulletin. "Literary Theory. Linguistics. Cultural Studies" Series 1 (2023): 155–68. http://dx.doi.org/10.28995/2686-7249-2023-1-155-168.

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In this article, I trace the various ways Ancient Greek artisans used seashell-motifs, and how they interpreted these natural forms in artistic contexts. The reason for the popularity of seashells lies in their visual and haptic variety, their wealth of association, and the device of something secret hidden under the hard outer shell. I examine three possible variants of the motif’s treatment in relief and volume: the shell-container as part of a vessel, the shell as an independent container, the shell as a sculpted motif on a vessel. Of particular interest are the vases where the integration

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Sahu, Santosh Kumar, Himadri Gourav Behuria, Sangam Gupta, and Babita Sahoo. "Sequence Analysis of a Subset of Plasma Membrane Raft Proteome Containing CXXC Metal Binding Motifs." International Journal of Knowledge Discovery in Bioinformatics 5, no. 2 (2015): 1–15. http://dx.doi.org/10.4018/ijkdb.2015070101.

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In an attempt to identify the metal sensing proteins localized to mammalian plasma membrane, the authors screened a list of 300 raft associated proteins that are involved in cellular signaling mechanisms by searching the presence of metal thionin (CXXC) motifs. 50 proteins were found to possess CXXC motifs that could act as potential metal sensing proteins. The authors determined membrane topologies of the above CXXC motif containing proteins using TM-pred and analyzed the positions of their transmembrane (TM) domains using Bio-edit software. Based on the topology of CXXC domains, the authors

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Goldhahn, Joakim. "Kosmologiska mantlar – strödda tankar kring hällbildsforskningens primitiva arv och epistemologi jämte bronsålderns kosmologi." In Situ Archaeologica 5 (December 31, 2003): 9–41. http://dx.doi.org/10.58323/insi.v5.12706.

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This article was originally presented at the 8th Nordic Bronze Age Seminar, held at the National Museum in Copenhagen back in 2000. It focuses on rock art interpretation in general and the history of research and interpretation of the so-called “cap” motifs in particular. Today, eleven different cap motifs are known in Scandinavia. I argue that researchers often tend to focus, isolate and overemphasise different single rock art motifs, or categories of motifs, and their connotations in an essentialistic search of their meaning. To put it simple: A cap is interpreted as a cap. It is also argued

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Solleder, Marthe, Philippe Guillaume, Julien Racle, et al. "Mass Spectrometry Based Immunopeptidomics Leads to Robust Predictions of Phosphorylated HLA Class I Ligands." Molecular & Cellular Proteomics 19, no. 2 (2019): 390–404. http://dx.doi.org/10.1074/mcp.tir119.001641.

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The presentation of peptides on class I human leukocyte antigen (HLA-I) molecules plays a central role in immune recognition of infected or malignant cells. In cancer, non-self HLA-I ligands can arise from many different alterations, including non-synonymous mutations, gene fusion, cancer-specific alternative mRNA splicing or aberrant post-translational modifications. Identifying HLA-I ligands remains a challenging task that requires either heavy experimental work for in vivo identification or optimized bioinformatics tools for accurate predictions. To date, no HLA-I ligand predictor includes

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19

Korvin, Gábor. "Muḥammad’s Jug: Arabic Motifs in Borges’s Texts". Arabist: Budapest Studies in Arabic 46 (2024): 103–47. http://dx.doi.org/10.58513/arabist.2024.46.4.

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In this study I discuss twenty-three Arabic motifs in Borges’s texts and trace them back to their most likely sources. Borges did not know Arabic and gained his knowledge of the Orient from secondary sources, books by Burton, Lane, Asín Palacios, and others. In many cases Borges playfully changed these Arabic motifs, or invented new ones. In the rare occasions when he gave his sources, these were as fantastic as the stories themselves: references to non-existing tomes by non-existing scholars, to an odd book of Burton (instead of the correct one by Lane), or to an out-of-context citation from

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Maziyah, Siti. "Motif Batik Tegal: Pengaruh Mataram, Pesisiran dan Islam." Endogami: Jurnal Ilmiah Kajian Antropologi 1, no. 2 (2018): 177. http://dx.doi.org/10.14710/endogami.1.2.177-193.

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BATIK -- motif is one of the cultural results that can show the identity of the region and has a unique cultural background. This article highlights the issue of Tegal Batik motif. What kind of batik Tegal is ? What cultures are behind the Tegal batik motifs? I apply the research method with historical approach to find the history of Tegal. Who are the actors in carrying out cultural changes in Tegal, especially related to the emergence of batik motifs. Based on the history, it will be known why Tegal batik has such motives. The results showed that based on the history of Tegal, batik motifs g

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Thuillard, Marc. "Analysis of the Worldwide Distribution of the ‘Man or Animal in the Moon’ Motifs." Folklore: Electronic Journal of Folklore 84 (December 2021): 127–44. http://dx.doi.org/10.7592/fejf2021.84.thuillard.

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For millennia, people have seen a man, an animal, or an object as they look at the moon. The motif of the ‘frog/toad in the Moon’ was recorded in writing in the Book of Changes (I Ching) over 2400 years ago. The ‘man in the Moon’ theme is found in old Norse literature in the Younger Edda. In Mesoamerica, the story of the ‘rabbit in the Moon’ is pre-Columbian. This study analyses the different versions by combining areal studies as well as structural and statistical analyses with information from ancient texts and archaeological artefacts. In particular, I compare the geographic distribution of

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Maslova, Anna G. "Biblical Motifs and Images in E. I. Kostrov’s Poetry." Проблемы исторической поэтики 27, no. 1 (2020): 92–110. http://dx.doi.org/10.15393/j9.art.2020.6882.

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<p>The article analyzes the peculiarities of biblical motifs and images in E.&nbsp;I.&nbsp;Kostrov&rsquo;s works. In his odes the poet often refers to the Old Testament scenes and images through the prism of a Christian aspect. Kostrov considers ancient and the Old Testament subjects in the light of the Christian and Orthodox tradition. The poet attributes a Christian sound to the secular genre of the ode, saturating his works with the motifs of the Holy Scriptures. Kostrov often uses the concept of &ldquo;meekness&rdquo; emphasizing the sanctity of the Orthodox power

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Wenzel, Jürgen J., Heidi Rossmann, Christian Fottner, et al. "Identification and Prevention of Genotyping Errors Caused by G-Quadruplex– and i-Motif–Like Sequences." Clinical Chemistry 55, no. 7 (2009): 1361–71. http://dx.doi.org/10.1373/clinchem.2008.118661.

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Abstract Background: Reliable PCR amplification of DNA fragments is the prerequisite for most genetic assays. We investigated the impact of G-quadruplex– or i-motif–like sequences on the reliability of PCR-based genetic analyses. Methods: We found the sequence context of a common intronic polymorphism in the MEN1 gene (multiple endocrine neoplasia I) to be the cause of systematic genotyping errors by inducing preferential amplification of one allelic variant [allele dropout (ADO)]. Bioinformatic analyses and pyrosequencing-based allele quantification enabled the identification of the underlyin

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Irving, Kelly L., Jessica J. King, Zoë A. E. Waller, Cameron W. Evans, and Nicole M. Smith. "Stability and context of intercalated motifs (i-motifs) for biological applications." Biochimie 198 (July 2022): 33–47. http://dx.doi.org/10.1016/j.biochi.2022.03.001.

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Chakraborty, Saikat, and Yamuna Krishnan. "Kinetic hybrid i-motifs: Intercepting DNA with RNA to form a DNA2–RNA2 i-motif." Biochimie 90, no. 7 (2008): 1088–95. http://dx.doi.org/10.1016/j.biochi.2008.02.022.

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Shi, Lili, Pai Peng, Jiao Zheng, et al. "I-Motif/miniduplex hybrid structures bind benzothiazole dyes with unprecedented efficiencies: a generic light-up system for label-free DNA nanoassemblies and bioimaging." Nucleic Acids Research 48, no. 4 (2020): 1681–90. http://dx.doi.org/10.1093/nar/gkaa020.

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Abstract I-motif DNAs have been widely employed as robust modulating components to construct reconfigurable DNA nanodevices that function well in acidic cellular environments. However, they generally display poor interactivity with fluorescent ligands under these complex conditions, illustrating a major difficulty in utilizing i-motifs as the light-up system for label-free DNA nanoassemblies and bioimaging. Towards addressing this challenge, here we devise new types of i-motif/miniduplex hybrid structures that display an unprecedentedly high interactivity with commonly-used benzothiazole dyes

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Rose, Nicola J., and Andrew M. L. Lever. "The rapamycin sensitivity of human T-cell leukaemia virus type I-induced T-cell proliferation is mediated independently of the polypyrimidine motifs in the 5′ long terminal repeat." Journal of General Virology 82, no. 2 (2001): 435–39. http://dx.doi.org/10.1099/0022-1317-82-2-435.

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The immunosuppressant rapamycin can regulate the translation of a subset of messenger RNAs, a phenotype which has been linked to the presence of a polypyrimidine motif [C(N)4–14] downstream of the mRNA cap structure. T-cell clones naturally infected with transcriptionally active human T-cell leukaemia virus, type I (HTLV-I) undergo autologous proliferation; this phenotype is inhibited by rapamycin but not FK506, which reverses the rapamycin effect. Within the R region of the HTLV-I 5′ long terminal repeat (LTR) there are seven polypyrimidine motifs. We sought to determine if these were involve

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Day, Henry Albert, Elisé Patricia Wright, Colin John MacDonald, Andrew James Gates, and Zoë Ann Ella Waller. "Reversible DNA i-motif to hairpin switching induced by copper(ii) cations." Chemical Communications 51, no. 74 (2015): 14099–102. http://dx.doi.org/10.1039/c5cc05111h.

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i-Motif forming DNA sequences have previously been used for many different nanotechnological applications, but all have used changes in pH to fold the DNA. Here it is shown that Cu(ii) cations can be used to re-fold i-motifs into hairpin structures, without changing the pH.

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Barry, Barbara. ""Where's my Fool?": Lear Motifs in Rigoletto." Comparative Drama 46, no. 1 (2012): 57–96. http://dx.doi.org/10.1353/cdr.2012.0001.

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Davenport, Miles P., Katherine J. Smith, Dan Barouch, et al. "HLA Class I Binding Motifs Derived from Random Peptide Libraries Differ at the COOH Terminus from Those of Eluted Peptides." Journal of Experimental Medicine 185, no. 2 (1997): 367–72. http://dx.doi.org/10.1084/jem.185.2.367.

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Recombinant HLA-A2, HLA-B8, or HLA-B53 heavy chain produced in Escherichia coli was combined with recombinant β2-microglobulin (β2m) and a pool of randomly synthesised nonamer peptides. This mixture was allowed to refold to form stable major histocompatability complex (MHC) class I complexes, which were then purified by gel filtration chromatography. The peptides bound to the MHC class I molecules were subsequently eluted and sequenced as a pool. Peptide binding motifs for these three MHC class I molecules were derived and compared with previously described motifs derived from analysis of natu

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Brzeski, J., T. Grycuk, A. W. Lipkowski, W. Rudnicki, B. Lesyng, and A. Jerzmanowski. "Binding of SPXK- and APXK-peptide motifs to AT-rich DNA. Experimental and theoretical studies." Acta Biochimica Polonica 45, no. 1 (1998): 221–31. http://dx.doi.org/10.18388/abp.1998_4304.

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The binding properties of the SPXK- and APXK-type peptides to the AT-rich DNA fragments of different length were studied by measuring the competition of peptides with Hoechst 33258 dye for DNA binding and by the gel shift assay analysis. In parallel to the experimental studies, molecular modeling techniques were used to analyze possible binding modes of the SPXZ and APXK motifs to the AT-rich DNA. The results of the competition measurements and gel shift assays suggest that serine at the i-1 position (i is proline) can be replaced by alanine without affecting the binding properties of the moti

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Liu, Mingfu, Raymond J. Turner, Tara L. Winstone, et al. "Escherichia coli TehB RequiresS-Adenosylmethionine as a Cofactor To Mediate Tellurite Resistance." Journal of Bacteriology 182, no. 22 (2000): 6509–13. http://dx.doi.org/10.1128/jb.182.22.6509-6513.2000.

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ABSTRACT The Escherichia coli chromosomal determinant for tellurite resistance consists of two genes (tehA andtehB) which, when expressed on a multicopy plasmid, confer resistance to K2TeO3 at 128 μg/ml, compared to the MIC of 2 μg/ml for the wild type. TehB is a cytoplasmic protein which possesses three conserved motifs (I, II, and III) found in S-adenosyl-l-methionine (SAM)-dependent non-nucleic acid methyltransferases. Replacement of the conserved aspartate residue in motif I by asparagine or alanine, or of the conserved phenylalanine in motif II by tyrosine or alanine, decreased resistance

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Jones, Christopher M. "“The Wealth of Nations Shall Come to You”: Light, Tribute, and Implacement in Isaiah 60." Vetus Testamentum 64, no. 4 (2014): 611–22. http://dx.doi.org/10.1163/15685330-12341178.

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I draw on spatial theory, and particularly Edward Casey’s concept of “implacement,” to investigate the rhetoric of Isaiah 60. Implacement means being concretely placed. I argue that Isaiah 60 uses the motifs of light and tribute to “implace” Jerusalem for its audience. It uses these motifs to acknowledge Jerusalem’s degraded state in the early fifth century and to imagine the means by which the city’s restoration will occur. Drawing on Wells’ work on inner-Isaianic allusion and Strawn’s argument that Isa 60 incorporates and subverts Persian iconography, I argue that, in Isa 60, the motif of li

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Delgado, Julio, Hernando Escobar, David Crockett, Eduardo Reyes-Vargas, and Peter Jensen. "Analysis of the performance of peptide predicting methods using MHC class I peptide sequencing in the C57BL/6 mouse (78.11)." Journal of Immunology 182, no. 1_Supplement (2009): 78.11. http://dx.doi.org/10.4049/jimmunol.182.supp.78.11.

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Abstract MHC class I peptide-binding motifs can defined by sequencing of peptides naturally bound to MHC class I molecules. More recently MHC class I-peptide binding motifs have be defined on the basis of quantitative MHC-peptide binding assays in vitro using peptide libraries. The information on peptide binding motifs obtained using binding assays have been useful to develop numerous bioinformatics-based tools to predict the binding of peptides to MHC class I molecules. To date few studies have analyzed the performance of these bioinformatics tools to predict peptides determined by sequencing

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Nakashima, Yoshiki, Hiroki Iguchi, Kenta Takakura, et al. "Adhesion Characteristics of Human Pancreatic Islets, Duct Epithelial Cells, and Acinar Cells to a Polymer Scaffold." Cell Transplantation 31 (January 2022): 096368972211205. http://dx.doi.org/10.1177/09636897221120500.

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We reported in 2018 that among several extracellular matrices, fibronectin, type I collagen, type IV collagen, laminin I, fibrinogen, and bovine serum albumin, fibronectin is particularly useful for adhesion of porcine pancreatic tissue. Subsequently, we developed a technology that enables the chemical coating of the constituent motifs of fibronectin onto cell culture dishes. In this experiment, we used islets (purity ≥ 90%), duct epithelial cells (purity ≥ 60%), and acinar cells (purity ≥ 99%) isolated from human pancreas according to the Edmonton protocol published in 2000 and achieved adhes

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Bielecka, Patrycja, Anna Dembska, and Bernard Juskowiak. "Monitoring of pH Using an i-Motif-Forming Sequence Containing a Fluorescent Cytosine Analogue, tC." Molecules 24, no. 5 (2019): 952. http://dx.doi.org/10.3390/molecules24050952.

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The i-motif is a four-stranded DNA structure formed from the cytosine (C)-rich ssDNA sequence, which is stabilized in slightly acidic pH. Additionally, labeling of a cytosine-rich sequence with a fluorescent molecule may constitute a way to construct a pH-sensitive biosensor. In this paper, we report tC-modified fluorescent probes that contain RET-related sequence C4GC4GC4GC4A. Results of the UV absorption melting experiments, circular dichroism (CD) spectra, and steady-state fluorescence measurements of tC-modified i-motifs are presented and discussed here. Efficient fluorescence quenching of

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Wildeman, A. G., M. Zenke, C. Schatz, et al. "Specific protein binding to the simian virus 40 enhancer in vitro." Molecular and Cellular Biology 6, no. 6 (1986): 2098–105. http://dx.doi.org/10.1128/mcb.6.6.2098-2105.1986.

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HeLa cell nuclear extracts and wild-type or mutated simian virus 40 enhancer DNA were used in DNase I footprinting experiments to study the interaction of putative trans-acting factors with the multiple enhancer motifs. We show that these nuclear extracts contain proteins that bind to these motifs. Because point mutations which are detrimental to the activity of a particular enhancer motif in vivo specifically prevent protection of that motif against DNase I digestion in vivo, we suggest that the bound proteins correspond to trans-acting factors involved in enhancement of transcription. Using

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Wildeman, A. G., M. Zenke, C. Schatz, et al. "Specific protein binding to the simian virus 40 enhancer in vitro." Molecular and Cellular Biology 6, no. 6 (1986): 2098–105. http://dx.doi.org/10.1128/mcb.6.6.2098.

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HeLa cell nuclear extracts and wild-type or mutated simian virus 40 enhancer DNA were used in DNase I footprinting experiments to study the interaction of putative trans-acting factors with the multiple enhancer motifs. We show that these nuclear extracts contain proteins that bind to these motifs. Because point mutations which are detrimental to the activity of a particular enhancer motif in vivo specifically prevent protection of that motif against DNase I digestion in vivo, we suggest that the bound proteins correspond to trans-acting factors involved in enhancement of transcription. Using

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Gross, Christian H., and Stewart Shuman. "The Nucleoside Triphosphatase and Helicase Activities of Vaccinia Virus NPH-II Are Essential for Virus Replication." Journal of Virology 72, no. 6 (1998): 4729–36. http://dx.doi.org/10.1128/jvi.72.6.4729-4736.1998.

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ABSTRACT Vaccinia virus NPH-II is the prototypal RNA helicase of the DExH box protein family, which is defined by six shared sequence motifs. The contributions of conserved amino acids in motifs I (TGVGKTSQ), Ia (PRI), II (DExHE), and III (TAT) to enzyme activity were assessed by alanine scanning. NPH-II-Ala proteins were expressed in baculovirus-infected Sf9 cells, purified, and characterized with respect to their RNA helicase, nucleic acid-dependent ATPase, and RNA binding functions. Alanine substitutions at Lys-191 and Thr-192 (motif I), Arg-229 (motif Ia), and Glu-300 (motif II) caused sev

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Ramírez Caballero, Lisbeth, Christoph Kny, Regina Treudler, et al. "Identification of Seasonal Variations of Antibodies against PR-10-Specific Epitopes Can Be Improved Using Peptide-Phage Display." International Archives of Allergy and Immunology 181, no. 12 (2020): 919–25. http://dx.doi.org/10.1159/000509995.

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<b><i>Background:</i></b> In pollinosis patients, allergen-specific antibody titers show seasonal variations. Little is known about these variations at the epitope level. <b><i>Objectives:</i></b> We aimed at investigating seasonal variations on the level of allergen epitope recognition in patients with Bet v 1-related food allergy using a peptide phage display approach. <b><i>Methods:</i></b> Serum samples collected over 1 year from 4 patients of the placebo arm of the birch-associated soya allergy immunotherapy trial wer

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Grishaeva, Tatiana M., Darya Kulichenko, and Yuri F. Bogdanov. "Bioinformatical analysis of eukaryotic shugoshins reveals meiosis-specific features of vertebrate shugoshins." PeerJ 4 (November 30, 2016): e2736. http://dx.doi.org/10.7717/peerj.2736.

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BackgroundShugoshins (SGOs) are proteins that protect cohesins located at the centromeres of sister chromatids from their early cleavage during mitosis and meiosis in plants, fungi, and animals. Their function is to prevent premature sister-chromatid disjunction and segregation. The study focused on the structural differences among SGOs acting during mitosis and meiosis that cause differences in chromosome behavior in these two types of cell division in different organisms.MethodsA bioinformatical analysis of protein domains, conserved amino acid motifs, and physicochemical properties of 32 pr

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Strobel, S. A. "Biochemical identification of A-minor motifs within RNA tertiary structure by interference analysis." Biochemical Society Transactions 30, no. 6 (2002): 1126–31. http://dx.doi.org/10.1042/bst0301126.

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A-minor motifs are the most common tertiary structural elements in RNA helix packing. Biochemical identification of these interactions is now feasible using interference mapping analysis with the adenosine analogues 2′-deoxyadenosine and 3-deaza-adenosine. This approach was used to demonstrate that A-minor motifs mediate helix packing interactions that are important for 5′-splice site selection in the group I intron. By analysing the interference pattern of several analogues it is possible to identify and distinguish the four variants of the A-minor motif.

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Litvinov, Rustem I., Marco Mravic, Hua Zhu, John W. Weisel, William F. DeGrado та Joel S. Bennett. "Unique transmembrane domain interactions differentially modulate integrin αvβ3 and αIIbβ3 function". Proceedings of the National Academy of Sciences 116, № 25 (2019): 12295–300. http://dx.doi.org/10.1073/pnas.1904867116.

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Lateral transmembrane (TM) helix–helix interactions between single-span membrane proteins play an important role in the assembly and signaling of many cell-surface receptors. Often, these helices contain two highly conserved yet distinct interaction motifs, arranged such that the motifs cannot be engaged simultaneously. However, there is sparse experimental evidence that dual-engagement mechanisms play a role in biological signaling. Here, we investigate the function of the two conserved interaction motifs in the TM domain of the integrin β3-subunit. The first motif uses reciprocating “large-l

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Heavin, Joshua. "Seon Yong Kim. Curse Motifs in Galatians." Bulletin for Biblical Research 32, no. 2 (2022): 243–48. http://dx.doi.org/10.5325/bullbiblrese.32.2.0243.

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Izquierdo, Adrian. ":La traduction fictive: Motifs d’un topos romanesque." Sixteenth Century Journal 53, no. 3 (2022): 815–17. http://dx.doi.org/10.1086/scj5303120.

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Bresnahan, Patricia A., Wes Yonemoto, and Warner C. Greene. "Cutting Edge: SIV Nef Protein Utilizes Both Leucine- and Tyrosine-Based Protein Sorting Pathways for Down-Regulation of CD4." Journal of Immunology 163, no. 6 (1999): 2977–81. http://dx.doi.org/10.4049/jimmunol.163.6.2977.

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Abstract The Nef protein is unique to primate lentiviruses and is closely linked to accelerated pathogenesis in both human and monkey hosts. Nef acts to down-regulate CD4 and MHC class I, two receptors important for immune function. A recent report demonstrated the presence of two tyrosine motifs in SIV Nef that contribute to its ability to down-regulate CD4 and to associate with clathrin adaptors. These tyrosine motifs are not present in HIV-1 Nef, which instead utilizes a leucine-based motif for its down-regulation of CD4. We now report that SIV Nef also contains a conserved leucine-based mo

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Gupta, Akshay. "An Intra-Hindu Comparative Analysis of Caitanya Vaiṣṇavism’s Eco-Theological Motifs". Journal of Dharma Studies 4, № 1 (2021): 5–27. http://dx.doi.org/10.1007/s42240-021-00098-y.

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AbstractIn the midst of the earth’s pressing climate catastrophe, the nexus between ecology and religion merits further investigation. In this article, I attempt to illuminate certain important aspects of this nexus by analyzing various Hindu eco-theological motifs, with a particular focus on those of the Hindu religious tradition known as Caitanya Vaiṣṇavism. I will compare and contrast the eco-theological motifs of Caitanya Vaiṣṇavism with those of other notable Hindu religious traditions and texts. Ultimately, I attempt to demonstrate that certain Caitanya Vaiṣṇava eco-theological motifs ca

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Choudhury, Manisha, Vijayan Viswanathan, Ajay Kumar Timiri, Barij Nayan Sinha, Venkatesan Jayaprakash, and Devadasan Velmurugan. "Crystal structures of 2-[(4,6-diaminopyrimidin-2-yl)sulfanyl]-N-(2,4-dimethylphenyl)acetamide and 2-[(4,6-diaminopyrimidin-2-yl)sulfanyl]-N-(3-methoxyphenyl)acetamide." Acta Crystallographica Section E Crystallographic Communications 73, no. 7 (2017): 996–1000. http://dx.doi.org/10.1107/s2056989017008143.

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In the title compounds, C14H17N5OS (I) and C13H15N5O2S (II), the dihedral angle between the pyrimidine and benzene rings is 58.64 (8)° in (I) and 78.33 (9)° in (II). In both compounds, there is an intramolecular C—H...O hydrogen bond, and in (II) there is also an intramolecular N—H...N hydrogen bond present. In the crystals of both compounds, a pair of N—H...N hydrogen bonds links the individual molecules to form inversion dimers withR22(8) ring motifs. In (I), the dimers are linked by N—H...O and C—H...O hydrogen bonds, enclosingR21(14),R21(11) andR21(7) ring motifs, forming layers parallel t

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Nadia Hasna, Safira Rizqi, and Mein Kharnolis. "Penerapan Motif Batik Papua dengan Teknik Bordir pada Busana Pengantin Wanita." BAJU: Journal of Fashion & Textile Design Unesa 2, no. 1 (2022): 18–23. http://dx.doi.org/10.26740/baju.v2n1.p18-23.

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Motif batik Papua yang berasal dari ragam hias khas Papua yang menjadi inspirasi dalam pembuatan busana pengantin wanita. Tujuan penelitian adalah untuk mengetahui proses dan hasil jadi penerapan motif batik papua dengan teknik bordir dibusana pengantin wanita. Metode yang digunakan adalah Double Diamond, yang terdiri dari 4 tahapan yaitu dicover, define, develop dan deliver. Dari hasil yang diperoleh, motif batik papua yaitu motif suku asmat yang diwujudkan teknik bordir digunakan sebagai detail di busana pengantin wanita. Proses pembuatan dengan cara membordir dengan mengikuti motif / pola y

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Belodedova, Margarita G. "“YOU PICK UP WHAT COMES TO MIND”. CONNECTED MOTIFS IN THE LULLABIES OF THE RUSSIAN NORTH." RSUH/RGGU Bulletin. "Literary Theory. Linguistics. Cultural Studies" Series, no. 6 (2023): 51–63. http://dx.doi.org/10.28995/2686-7249-2023-6-51-63.

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Traditional lullabies do not have a common plot – they are a bundle of a number of micro-plots that the performer “pulls” together, each time creating a new song. Each lullaby will be unique not only in the volume of the text, but also in the number of motifs, their coiled-uncoiled, their combination and sequence. Despite the improvisational nature of the genre, lullabies consist of motifs so stable and have such a rigid and clichéd rhythmic structure that these motifs can be called formulas. They can have both a rather capacious, brief form, and a more expanded structure – one of these forms

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