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Artykuły w czasopismach na temat „Investigation of antimicrobial susceptibility of E. cloacae”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

GONÇALVES, Célia R., Tania M. I. VAZ, Daniela LEITE, et al. "Molecular epidemiology of a nosocomial outbreak due to Enterobacter cloacae and Enterobacter agglomerans in Campinas, São Paulo, Brazil." Revista do Instituto de Medicina Tropical de São Paulo 42, no. 1 (2000): 1–7. http://dx.doi.org/10.1590/s0036-46652000000100001.

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A total of 73 isolates (57 Enterobacter cloacae and 16 Enterobacter agglomerans), recovered during an outbreak of bacteremia in the Campinas area, São Paulo, Brazil, were studied. Of these isolates, 61 were from parenteral nutrition solutions, 9 from blood cultures, 2 from a sealed bottle of parenteral nutrition solution, and one was of unknown origin. Of the 57 E. cloacae isolates, 54 were biotype 26, two were biotype 66 and one was non-typable. Of 39 E. cloacae isolates submitted to ribotyping, 87.2% showed the same banding pattern after cleavage with EcoRI and BamHI. No important difference

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Álvaro, Bustos González, Ortiz Girón José, Diaz Cornejo Nohra, Galván Garces Olga, and Ruiz Garcés Luis. "Resistance profiles of bacterial pathogens isolated in a hospital institution in Montería - Córdoba, 2018." Indian Journal of Science and Technology 13, no. 46 (2020): 4564–72. https://doi.org/10.17485/IJST/v13i46.1897.

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Abstract <strong>Objective:</strong> To determine the resistance profiles of bacterial pathogens isolated in a hospital institution in Montería – Córdoba. <strong>Methodology:</strong> An observational study was carried out, during the months of January to December 2018; 344 samples were evaluated. Mention about the source of study samples. The identification and susceptibility of the bacteria were determined through the VITEK system. <strong>Findings:</strong> Among the Gram-negative bacteria identified were Klebsiella pneumoniae, Pseudomonas aerug

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Nohra, Diaz-Cornejo, Ruiz-Garcés Luis, Ortiz-Girón José, and RamirezBarbera Nathalie. "Antimicrobial resistance profiles of bacterial pathogens isolated from the bloodstream in patients of a hospital institution in Montería — Córdoba." Indian Journal of Science and Technology 13, no. 19 (2020): 1908–16. https://doi.org/10.17485/IJST/v13i19.349.

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Abstract <strong>Objectives:</strong> To determine the most frequent bacterial pathogens in the bloodstream and their resistance profiles in a hospital institution in Monteria - Cordoba. <strong>Methods:</strong> An observational study was conducted, during the months of January to December 2018, 113 blood samples from patients with sepsis were evaluated. The identification and susceptibility of the bacteria were determined through the VITEK system, using the GNI + cards for Gram-negative bacilli and GPI for Gram-positive cocci and the GNS 113 and GPS 102 cards for Gram-negative

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4

Cardona-Cabrera, Teresa, Sandra Martínez-Álvarez, Carmen González-Azcona, et al. "High Antimicrobial Susceptibility of Cloacal Enterococci and Escherichia coli from Free-Living Dalmatian and Great White Pelicans with Detection of Cefotaximase CTX-M-15 Producing Escherichia coli ST69." Antibiotics 14, no. 1 (2025): 83. https://doi.org/10.3390/antibiotics14010083.

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Background/Objectives: In 2022, an outbreak of H5N1 highly pathogenic avian influenza (HPAI) killed 60% of the largest breeding colony of Dalmatian pelicans (DPs) in the world at Mikri Prespa Lake (Greece), prompting a multidisciplinary study on HPAI and other pathogens. This study determines the antimicrobial resistance rates of cloacal enterococci and Escherichia coli in DPs. Methods: Fifty-two blood and cloacal swab samples were collected from 31 nestlings (20 DP/11 great white pelicans) hatched after the H5N1 outbreak at the Prespa colony and 21 subadult/adult DPs captured at a spring migr

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Silva, Gedean Galdino da Cruz, Eloiza Helena Campana, Priscylla Carvalho Vasconcelos, et al. "Occurrence of KPC-Producing Escherichia coli in Psittaciformes Rescued from Trafficking in Paraíba, Brazil." International Journal of Environmental Research and Public Health 18, no. 1 (2020): 95. http://dx.doi.org/10.3390/ijerph18010095.

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The emergence and spread of antimicrobial resistance pose a threat to public health globally. Antibiotic-resistant bacteria and genes can disseminate among environments, animals and humans. Therefore, investigation into potential reservoirs of multidrug-resistant bacteria is of great importance to the understanding of putative transmission routes of resistant bacteria and resistance genes. This study aimed to report the occurrence of Escherichia coli harboring the Klebsiella pneumoniae carbapenemase-producing gene (blaKPC) in Psittaciformes rescued from wildlife trafficking in Paraíba State, B

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Alzahrani, Othman M., Fakhur Uddin, Samy F. Mahmoud, Amal S. Alswat, Muhammad Sohail, and Mona Youssef. "Resistance to Some New Drugs and Prevalence of ESBL- and MBL-Producing Enterobacteriaceae Uropathogens Isolated from Diabetic Patients." Life 12, no. 12 (2022): 2125. http://dx.doi.org/10.3390/life12122125.

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Diabetes is a leading non-communicable disease and a risk factor for relapsing infections. The current study was aimed at investigating the prevalence and antibiotic susceptibility of carbapenem-resistant (CR) uropathogens of the family Enterobacteriaceae in diabetic patients. The data of 910 bacterial isolates was collected from diagnostic laboratories during January 2018 to December 2018. The bacterial isolates were identified using traditional methods including colonial characteristics, biochemical tests, and API (20E). Antimicrobial susceptibility and phenotypic characterization of ESBL, M

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Motta, Rodrigo G., Lorrayne S. A. Martins, Igor G. Motta, et al. "Multidrug resistant bacteria isolated from septic arthritis in horses." Pesquisa Veterinária Brasileira 37, no. 4 (2017): 325–30. http://dx.doi.org/10.1590/s0100-736x2017000400005.

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ABSTRACT: Septic arthritis is a debilitating joint infectious disease of equines that requires early diagnosis and immediate therapeutic intervention to prevent degenerative effects on the articular cartilage, as well as loss of athletic ability and work performance of the animals. Few studies have investigated the etiological complexity of this disease, as well as multidrug resistance of isolates. In this study, 60 horses with arthritis had synovial fluid samples aseptically collected, and tested by microbiological culture and in vitro susceptibility test (disk diffusion) using nine antimicro

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Morhart, Patrick, Roman G. Gerlach, Caroline Kunz, et al. "Application of Next-Generation Sequencing to Enterobacter Hormaechei Subspecies Analysis during a Neonatal Intensive Care Unit Outbreak." Children 10, no. 10 (2023): 1696. http://dx.doi.org/10.3390/children10101696.

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Introduction: The Enterobacter cloacae complex (ECC) species are potential neonatal pathogens, and ECC strains are among the most commonly encountered Enterobacter spp. associated with nosocomial bloodstream infections. Outbreaks caused by ECC can lead to significant morbidity and mortality in susceptible neonates. At the molecular level, ECC exhibits genomic heterogeneity, with six closely related species and subspecies. Genetic variability poses a challenge in accurately identifying outbreaks by determining the clonality of ECC isolates. This difficulty is further compounded by the limitatio

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9

Grass, Julian E., Shelley S. Magill, Isaac See, et al. "Evaluation of Discrepancies in Carbapenem Minimum Inhibitory Concentrations Obtained at Clinical Laboratories Compared to a Public Health Laboratory." Infection Control & Hospital Epidemiology 41, S1 (2020): s474—s476. http://dx.doi.org/10.1017/ice.2020.1151.

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Background: Automated testing instruments (ATIs) are commonly used by clinical microbiology laboratories to perform antimicrobial susceptibility testing (AST), whereas public health laboratories may use established reference methods such as broth microdilution (BMD). We investigated discrepancies in carbapenem minimum inhibitory concentrations (MICs) among Enterobacteriaceae tested by clinical laboratory ATIs and by reference BMD at the CDC. Methods: During 2016–2018, we conducted laboratory- and population-based surveillance for carbapenem-resistant Enterobacteriaceae (CRE) through the CDC Em

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10

Harada, Tetsuya, Ryoko Yamane, Van Chinh Dang, et al. "Prevalence and Antimicrobial Susceptibility of Enterobacteriaceae Isolated from Retail Pepper in Vietnam." Journal of Food Protection 80, no. 5 (2016): 716–24. http://dx.doi.org/10.4315/0362-028x.jfp-16-501.

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ABSTRACT To investigate the microbial quality of retail pepper in Vietnam, the enumeration and detection of Enterobacteriaceae and the screening of cefotaxime (CTX)–resistant coliforms were performed by using 84 commercial samples. Although Enterobacteriaceae were isolated from 78 samples, the number of Enterobacteriaceae was lower than 1.0 log CFU/g in 46 samples. For the detection of Enterobacteriaceae with the International Organization for Standardization methods, Salmonella spp., Escherichia coli, Klebsiella pneumoniae, Cronobacter sakazakii, and Enterobacter cloacae complex were isolated

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11

Johnson, Wesley, David Burgess, Donna Burgess, et al. "Impact of CLSI Break Point Changes Over the Past Decade on Antimicrobial Susceptibility in Gram-Negative Bacteria." Antimicrobial Stewardship & Healthcare Epidemiology 1, S1 (2021): s60. http://dx.doi.org/10.1017/ash.2021.115.

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Background: Over the past decade, the CLSI has updated susceptibility break points for several antimicrobial agents. The purpose of this study was to evaluate the impact of these changes against gram-negative bacteria at our academic medical center. Methods: In this retrospective, IRB-approved study, we collected consecutive, nonduplicate clinical isolates of Enterobacter cloacae, Escherichia coli, Klebsiella aerogenes, K. oxytoca, K. pneumoniae, and Pseudomonas aeruginosa for the past decade (2010–2019) at our academic medical center and 3 adult ICUs. Susceptibility testing was performed usin

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12

Colak, Meryem, and Nergis Asgin. "Antimicrobial resistance profiles of Enterobacter cloacae and Klebsiella aerogenes a tertiary hospital in Turkey: A five-years study." Medical Science and Discovery 8, no. 3 (2021): 161–66. http://dx.doi.org/10.36472/msd.v8i3.501.

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Objective: Enterobacter cloacae and Klebsiella aerogenes species have multiple drug resistance and antibiotic resistance is a growing problem regarding to treating infections. Objective: The aim of this study was to determine and evaluate the antimicrobial resistance profiles of E. cloacae and K. aerogenes isolated from various clinical samples in our laboratory, retrospectively. Material and Methods: Totally 223 patients who applied to Karabuk University Training and Research Hospital microbiology laboratory between October2016-December2020 were included in this study. Conventiona

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13

Rapp, Robert P., Julie A. Ribes, Sue B. Overman, Theodore E. Darkow, and Martin E. Evans. "A Decade of Antimicrobial Susceptibilities at the University of Kentucky Hospital." Annals of Pharmacotherapy 36, no. 4 (2002): 596–604. http://dx.doi.org/10.1345/aph.1a249.

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OBJECTIVE: To determine the antimicrobial susceptibility rates for key antimicrobial agents and selective bacterial pathogens in the decade of the 1990s. METHODS: Data from 1990 to 2000 from the University of Kentucky Clinical Microbiology Laboratory were analyzed by linear regression analysis to identify agents and pathogens that show a decline in susceptibility. For selected pathogens and antimicrobial agents, predictions were made for further declines in susceptibility for 2005 and 2010. RESULTS: Significant declines in susceptibility to selected antimicrobial agents were found for Pseudomo

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14

Pontes, Daniela Santos, Cláudia Iracema Lima-Bittencourt, Marcela Santiago Pacheco Azevedo, Edmar Chartone-Souza, and Andréa Maria Amaral Nascimento. "Phenotypic and genetic analysis of Enterobacter spp. from a Brazilian oligotrophic freshwater lake." Canadian Journal of Microbiology 53, no. 8 (2007): 983–91. http://dx.doi.org/10.1139/w07-060.

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We characterized a population of Enterobacter spp. of the Enterobacter cloacae complex isolated from an oligotrophic lake; most isolates were identified as E. cloacae. Fingerprinting polymerase chain reaction (PCR), along with morphological, biochemical, physiological, and plasmid profiles analyses, including antimicrobial susceptibility testing, were performed on 22 environmental isolates. Misidentification occurred when using the API 20E identification system. Analysis of 16S rDNA sequences confirmed the close relatedness between species of the E. cloacae complex. The tDNA PCR allowed the di

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15

Gaona, Marc, Jordi Corral, Miquel Sánchez−Osuna, et al. "Reciprocal regulation between Acinetobacter baumannii and Enterobacter cloacae AdeR homologs: implications for antimicrobial resistance and pathogenesis." PLOS ONE 20, no. 3 (2025): e0315428. https://doi.org/10.1371/journal.pone.0315428.

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Acinetobacter baumannii and Enterobacter cloacae are phylogenetically distant Gram−negative bacterial pathogens that represent significant challenges in healthcare settings due to their remarkable ability to acquire antimicrobial resistance. This study investigates one of the most important efflux pump systems in A. baumannii, AdeABC−AdeRS, and identifies homologous components in E. cloacae. By constructing isogenic knockout mutants, we show that the AdeB pump component and the AdeR regulator are significant for antimicrobial resistance and pathogenicity in A. baumannii. Through in silico pred

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16

Shecho, Mude, Naod Thomas, Jelalu Kemal, and Yimer Muktar. "Cloacael Carriage and Multidrug Resistance Escherichia coli O157:H7 from Poultry Farms, Eastern Ethiopia." Journal of Veterinary Medicine 2017 (February 27, 2017): 1–9. http://dx.doi.org/10.1155/2017/8264583.

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A cross-sectional study was carried out to determine antimicrobial drug resistance patterns of E. coli O157:H7 isolates and estimate the level of the pathogen. A total of 194 cloacae swab samples were collected randomly in two poultry farms. Standard cultural, biochemical, and serological (latex agglutination) methods were used to isolate E. coli O157:H7. The isolates were subjected to antimicrobial susceptibility testing using disc diffusion method. Out of 194 cloacae samples examined, 13.4% (n=26) were found to be positive for E. coli O157:H7. The finding indicated differences in E. coli O15

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17

Lawal, Jallailudeen Rabana, Juliana James Ndahi, Amina Mohammed Bello, Yakaka Wakil, Jamila Dauda, and Umar Isa Ibrahim. "Prevalence, isolation and antimicrobial susceptibility of Gallibacterium anatis from Local Breed of Female Muscovy Ducks (Cairina moschata) in Maiduguri, Northeastern Nigeria." Journal of Animal Science and Veterinary Medicine 2, no. 2 (2017): 27–37. http://dx.doi.org/10.31248/jasvm2017.042.

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The present study was carried out from the month of March to December, 2016 and aimed to isolate and investigate the prevalence of Gallibacterium anatis infection as well as to study the antimicrobial susceptibility pattern of the bacterium from extensively reared local breed of female Muscovy ducks in Maiduguri, Northeastern Nigeria. To accomplish this, a total of 250 samples (100 tracheal swabs, 100 cloacal swabs and 50 part of ovary) were collected from households where Muscovy ducks are reared and female Muscovy ducks from live birds market. Microbiological isolation and biochemical identi

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Fadlallah, Mahdi, and Elie Salem Sokhn. "Epidemiology and resistance profiles of Enterobacterales in a tertiary care hospital in Lebanon: a 4-year retrospective study." Journal of Infection in Developing Countries 17, no. 07 (2023): 986–93. http://dx.doi.org/10.3855/jidc.17313.

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Introduction: Antimicrobial resistance (AMR) is a worldwide problem that threatens treatment effectiveness against the most serious bacterial infections. AMR in Enterobacterales is highly prevalent in Lebanon. However, recent reports regarding the distribution of Enterobacterales and related antimicrobial susceptibility are scarce. Methodology: In this retrospective study at the Lebanese Hospital Geitaoui Medical Center in Lebanon, all data regarding culture specimens from urine, blood, sputum, deep tracheal aspirate, broncho-alveolar lavage, wounds, surgical sites, tissue, body fluids, and ce

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Adamson, Peter J. W., W. David Wilson, Dwight C. Hirsh, J. Desmond Baggot, and Lori D. Martin. "Susceptibility of equine bacterial isolates to antimicrobial agents." American Journal of Veterinary Research 46, no. 2 (1985): 447–50. https://doi.org/10.2460/ajvr.1985.46.02.447.

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SUMMARY In vitro antimicrobic susceptibility patterns of commonly isolated aerobic gram-positive and gram-negative bacterial pathogens of equine origin were determined, using the agar-plate dilution method. All organisms were recent clinical isolates and included Corynebacterium (Rhodococcus) equi, Corynebacterium pseudotuberculosis, (coagulase positive) Staphylococcus sp, Streptococcus equi, Streptococcus zooepidemicus, Actinobacillus sp, Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Salmonella. In vitro susceptibility levels were outlined for 14 a

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Mbehang Nguema, Pierre Philippe, Richard Onanga, Guy Roger Ndong Atome, et al. "High level of intrinsic phenotypic antimicrobial resistance in enterobacteria from terrestrial wildlife in Gabonese national parks." PLOS ONE 16, no. 10 (2021): e0257994. http://dx.doi.org/10.1371/journal.pone.0257994.

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Data on the prevalence of antibiotic resistance in Enterobacteriaceae in African wildlife are still relatively limited. The aim of this study was to estimate the prevalence of phenotypic intrinsic and acquired antimicrobial resistance of enterobacteria from several species of terrestrial wild mammals in national parks of Gabon. Colony culture and isolation were done using MacConkey agar. Isolates were identified using the VITEK 2 and MALDI-TOF methods. Antibiotic susceptibility was analysed and interpreted according to the European Committee on Antimicrobial Susceptibility Testing guidelines.

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Waites, Ken B., Lynn B. Duffy, and Michael J. Dowzicky. "Antimicrobial Susceptibility among Pathogens Collected from Hospitalized Patients in the United States and In Vitro Activity of Tigecycline, a New Glycylcycline Antimicrobial." Antimicrobial Agents and Chemotherapy 50, no. 10 (2006): 3479–84. http://dx.doi.org/10.1128/aac.00210-06.

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ABSTRACT The activities of tigecycline and comparators against isolates collected from 76 U.S. centers between January 2004 and September 2005 were assessed. Tigecycline MIC90s were ≤2 μg/ml for Klebsiella pneumoniae, Klebsiella oxytoca, Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae, Serratia marcescens, Acinetobacter baumannii, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium, and Streptococcus agalactiae.

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Ojulong, Julius, Gebrekrstos N. Gebru, Babatunde Duduyemi, et al. "Prevalence of Antimicrobial Resistance in Klebsiella pneumoniae, Enterobacter cloacae, and Escherichia coli Isolates among Stillbirths and Deceased Under-Five Children in Sierra Leone: Data from the Child Health and Mortality Prevention Surveillance Sites from 2019 to 2022." Microorganisms 12, no. 8 (2024): 1657. http://dx.doi.org/10.3390/microorganisms12081657.

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Klebsiella pneumoniae, Escherichia coli, and Enterobacter cloacae are associated with most nosocomial infections worldwide. Although gaps remain in the knowledge of their susceptibility patterns, these are in antimicrobial stewardship. This study aimed to describe antimicrobial susceptibility profiles of the above organisms isolated from postmortem blood from stillbirths and under-five children enrolled in the Child Health and Mortality Prevention Surveillance (CHAMPS) program in Sierra Leone. This was a surveillance study of bacteria isolates from postmortem blood cultures taken within 24 h o

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Piccirilli, Alessandra, Elisa Meroni, Carola Mauri, et al. "Analysis of Antimicrobial Resistance Genes (ARGs) in Enterobacterales and A. baumannii Clinical Strains Colonizing a Single Italian Patient." Antibiotics 12, no. 3 (2023): 439. http://dx.doi.org/10.3390/antibiotics12030439.

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The dramatic increase in infections caused by critically multidrug-resistant bacteria is a global health concern. In this study, we characterized the antimicrobial resistance genes (ARGs) of K. pneumoniae, P. mirabilis, E. cloacae and A. baumannii isolated from both surgical wound and rectal swab of a single Italian patient. Bacterial identification was performed by MALDI-TOF MS and the antimicrobial susceptibility was carried out by Vitek 2 system. The characterization of ARGs was performed using next-generation sequencing (NGS) methodology (MiSeq Illumina apparatus). K. pneumoniae, P. mirabi

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Garza-González, Elvira, Sandra Iveth Mendoza Ibarra, Jorge M. Llaca-Díaz та Gloria M. Gonzalez. "Molecular characterization and antimicrobial susceptibility of extended-spectrum β-lactamase-producing Enterobacteriaceae isolates at a tertiary-care centre in Monterrey, Mexico". Journal of Medical Microbiology 60, № 1 (2011): 84–90. http://dx.doi.org/10.1099/jmm.0.022970-0.

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Our objective was to analyse phenotypic and genetic data of extended-spectrum β-lactamase (ESBL)-producing Klebsiella pneumoniae, Enterobacter cloacae, Escherichia coli and Serratia marcescens that cause infections in our hospital. Over a 3 year period, 342 randomly selected clinical Enterobacteriaceae isolates were tested for ESBL production and evaluated for the presence of the β-lactamase genes bla SHV, bla TEM, bla CTX-M and bla TLA-1. The antibiotic susceptibilities of these isolates were also determined, and the clonality of the isolates was assessed by PFGE. Based on our analyses, 33/92

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ARYAL, Prerit Upadhyaya, Benjamas THAMJARUNGWONG, Kamonnut SINGKHAMANAN, et al. "Emergence of Carbapenem-Resistant Enterobacteriaceae in a Tertiary Care Hospital in Southern Thailand†." Walailak Journal of Science and Technology (WJST) 17, no. 10 (2020): 1139–48. http://dx.doi.org/10.48048/wjst.2020.10729.

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Carbapenem-resistant Enterobacteriaceae (CRE) is emerging as a major problem in healthcare settings globally, including Thailand, due to limited therapeutic options. We reported the detection, antimicrobial susceptibility profiles, and the presence of carbapenemase genes of CRE isolates obtained from Songklanagarind Hospital between July 2012 and June 2015. A total of 273 non-duplicated CRE isolates was recovered from 248 patients. The predominant organism was Klebsiella pneumoniae (183 [67.0 %]), followed by Escherichia coli (38 [13.9 %]). The susceptibility to 13 antibiotics was performed by

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Pérez, Astrid, Delia Canle, Cristina Latasa, et al. "Cloning, Nucleotide Sequencing, and Analysis of the AcrAB-TolC Efflux Pump of Enterobacter cloacae and Determination of Its Involvement in Antibiotic Resistance in a Clinical Isolate." Antimicrobial Agents and Chemotherapy 51, no. 9 (2007): 3247–53. http://dx.doi.org/10.1128/aac.00072-07.

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ABSTRACT Enterobacter cloacae is an emerging clinical pathogen that may be responsible for nosocomial infections. Management of these infections is often difficult, owing to the high frequency of strains that are resistant to disinfectants and antimicrobial agents in the clinical setting. Multidrug efflux pumps, especially those belonging to the resistance-nodulation-division family, play a major role as a mechanism of antimicrobial resistance in gram-negative pathogens. In the present study, we cloned and sequenced the genes encoding an AcrAcB-TolC-like efflux pump from an E. cloacae clinical

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Jepsen, Karin, Wolfgang Falk, Friederike Brune, et al. "Prevalence and Antibiotic Susceptibility Trends of Selected Enterobacteriaceae, Enterococci, and Candida albicans in the Subgingival Microbiota of German Periodontitis Patients: A Retrospective Surveillance Study." Antibiotics 11, no. 3 (2022): 385. http://dx.doi.org/10.3390/antibiotics11030385.

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The periodontal microbiota is ecologically diverse and may facilitate colonization by bacteria of enteric origin (Enterobacteriaceae, Enterococci) and co-infections with Candida albicans, possibly producing subgingival biofilms with high antimicrobial tolerance. This retrospective surveillance study followed periodontitis-associated superinfection profiles in a large patient sample. From 2008 to 2015, biofilm samples from deep periodontal pockets were collected from a total of 16,612 German adults diagnosed with periodontitis. The presence of selected Enterobacteriaceae, Enterococci, and Candi

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Martha Echioda- Ogbole and Enid Godwin. "Occurrence and Antimicrobial Susceptibility Profiles of Salmonella species among Chickens of some Commercial Poultry Farms in Benue State, Nigeria." UMYU Journal of Microbiology Research (UJMR) 4, no. 1 (2019): 39–44. http://dx.doi.org/10.47430/ujmr.1941.007.

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Salmonella is a facultative anaerobic Gram negative rod, an intracellular bacterium of zoonotic importance. This study aimed at investigating the presence of Salmonella in chickens in three local government areas of Benue State. A total of 588 cloacal swabs were made randomly from 264 layers and 324 broilers at Makurdi, Otukpo and Gboko local government areas of Benue State, from March to July 2015. Salmonellae were isolated and identified using standard bacteriological techniques namely culture, Gram’s staining and biochemical methods. Cultural characteristics of isolates on the used media we

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Lu, Ching-Lan, Chia-Ying Liu, Yu-Tsung Huang, et al. "Antimicrobial Susceptibilities of Commonly Encountered Bacterial Isolates to Fosfomycin Determined by Agar Dilution and Disk Diffusion Methods." Antimicrobial Agents and Chemotherapy 55, no. 9 (2011): 4295–301. http://dx.doi.org/10.1128/aac.00349-11.

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ABSTRACTWe studied the antimicrobial activity of fosfomycin against 960 strains of commonly encountered bacteria associated with urinary tract infection using standard agar dilution and disk diffusion methods. Species studied included 3 common species ofEnterobacteriaceae,Pseudomonas aeruginosa,Acinetobacter baumannii, andStenotrophomonas maltophilia; methicillin-susceptible and -resistantStaphylococcus aureus; and vancomycin-susceptible and resistantEnterococcus faecalisandE. faecium. MICs and inhibition zone diameters were interpreted in accordance with both the currently recommended Clinica

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Turnidge, John D., Thomas Gottlieb, David H. Mitchell, Geoffrey W. Coombs, Denise A. Daley, and Jan M. Bell. "Enterobacteriaceae Sepsis Outcome Programme annual report, 2013." Communicable Diseases Intelligence 38 (December 1, 2014): 327–33. https://doi.org/10.33321/cdi.2014.38.53.

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The Australian Group on Antimicrobial Resistance performs regular period-prevalence studies to monitor changes in antimicrobial resistance in selected enteric Gram-negative pathogens. The 2013 survey focussed for the first time on blood stream infections. Four thousand nine hundred and fifty-eight Enterobacteriaceae species were tested using commercial automated methods (Vitek® 2, BioMérieux; Phoenix™, BD). The results were analysed using Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints (January 2014). Of the

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Bell, Jan M., John D. Turnidge, Geoffrey W. Coombs, et al. "Australian Group on Antimicrobial Resistance Australian Enterobacteriaceae Sepsis Outcome Programme annual report, 2014." Communicable Diseases Intelligence 40 (June 1, 2016): 229–35. https://doi.org/10.33321/cdi.2016.40.18.

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The Australian Group on Antimicrobial Resistance performs regular period-prevalence studies to monitor changes in antimicrobial resistance in selected enteric Gram-negative pathogens. The 2014 survey was the second year to focus on blood stream infections. During 2014, 5,798 Enterobacteriaceae species isolates were tested using commercial automated methods (Vitek 2, BioMérieux; Phoenix, BD) and results were analysed using the Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints (January 2015). Of the key resistan

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Rammohan Chitra, Subbiah. "Theoretical Investigation on Antimicrobial Susceptibility Testing Methods." Computational Biology and Bioinformatics 5, no. 2 (2017): 12. http://dx.doi.org/10.11648/j.cbb.20170502.11.

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Wang, Su, Shu-Zhen Xiao, Fei-Fei Gu, et al. "Antimicrobial susceptibility and molecular epidemiology of clinical Enterobacter cloacae bloodstream isolates in Shanghai, China." PLOS ONE 12, no. 12 (2017): e0189713. http://dx.doi.org/10.1371/journal.pone.0189713.

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Perovic, Olga, Husna Ismail, Erika Van Schalkwyk, et al. "Antimicrobial resistance surveillance in the South African private sector report for 2016." Southern African Journal of Infectious Diseases 33, no. 4 (2018): 114–17. http://dx.doi.org/10.4102/sajid.v33i4.160.

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Aim: The relevance of surveillance for antimicrobial resistance is increasingly recognised in the light of a global action plan to combat resistance. This report presents antimicrobial susceptibility testing on ESKAPE pathogens from private sector laboratories in South Africa for 2016.Methods: Antimicrobial susceptibility testing (AST) performed on ESKAPE organisms (Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae and Escherichia coli) isolated from blood cultures at four private pat

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Meerwein, Milton, Andrea Tarnutzer, Michelle Böni, Françoise Van Bambeke, Michael Hombach, and Annelies S. Zinkernagel. "Increased Azithromycin Susceptibility of Multidrug-Resistant Gram-Negative Bacteria on RPMI-1640 Agar Assessed by Disk Diffusion Testing." Antibiotics 9, no. 5 (2020): 218. http://dx.doi.org/10.3390/antibiotics9050218.

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Increasing antibiotic resistances and a lack of new antibiotics render the treatment of Gram-negative bacterial infections increasingly difficult. Therefore, additional approaches are being investigated. Macrolides are not routinely used against Gram-negative bacteria due to lack of evidence of in vitro effectiveness. However, it has been shown that Pseudomonas spp. are susceptible to macrolides in liquid RPMI-1640 and clinical data suggest improvement in patients’ outcomes. So far, these findings have been hardly applicable to the clinical setting due to lack of routine low-complexity antimic

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Pérez, Astrid, Margarita Poza, Jesús Aranda, et al. "Effect of Transcriptional Activators SoxS, RobA, and RamA on Expression of Multidrug Efflux Pump AcrAB-TolC in Enterobacter cloacae." Antimicrobial Agents and Chemotherapy 56, no. 12 (2012): 6256–66. http://dx.doi.org/10.1128/aac.01085-12.

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ABSTRACTControl of membrane permeability is a key step in regulating the intracellular concentration of antibiotics. Efflux pumps confer innate resistance to a wide range of toxic compounds such as antibiotics, dyes, detergents, and disinfectants in members of theEnterobacteriaceae. The AcrAB-TolC efflux pump is involved in multidrug resistance inEnterobacter cloacae. However, the underlying mechanism that regulates the system in this microorganism remains unknown. InEscherichia coli, the transcription ofacrABis upregulated under global stress conditions by proteins such as MarA, SoxS, and Rob

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Isawumi, Abiola, Molly Kukua Abban, Eunice Ampadubea Ayerakwa, and Lydia Mosi. "Calcium Potentiated Carbapenem Effectiveness Against Resistant Enterobacter Species." Microbiology Insights 15 (January 2022): 117863612211337. http://dx.doi.org/10.1177/11786361221133728.

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Background: Antimicrobial resistance (AMR) remains a global health challenge, as bacteria display increasing resistance to last-resort antibiotics such as carbapenems. Enterobacter cloacae are evolving and developing high level of resistance to carbapenems. With increasing AMR, availability of antibiotics for treatment dwindles, hence a need to complement antibiotics to enhance activity or reduce the level of resistance. This study explored the use of calcium ions in attenuating bacterial resistance to carbapenems. Method: E. cloacae strains isolated from hospital fomites and air were subjecte

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Karlowsky, James A., Mark E. Jones, Clyde Thornsberry, Ian R. Friedland, and Daniel F. Sahm. "Trends in Antimicrobial Susceptibilities among Enterobacteriaceae Isolated from Hospitalized Patients in the United States from 1998 to 2001." Antimicrobial Agents and Chemotherapy 47, no. 5 (2003): 1672–80. http://dx.doi.org/10.1128/aac.47.5.1672-1680.2003.

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ABSTRACT Longitudinal surveillance of Enterobacteriaceae for antimicrobial susceptibility is important because species of this family are among the most significant and prevalent human pathogens. To estimate rates of in vitro antimicrobial susceptibility among hospitalized patients in the United States, data from The Surveillance Network were studied for 14 agents tested against 10 species of Enterobacteriaceae (n = 384,279) isolated from intensive-care-unit (ICU) patients and non-ICU inpatients from 1998 to 2001. Cumulative susceptibility (percent) data for all species of Enterobacteriaceae i

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Pérez, Astrid, Margarita Poza, Ana Fernández, et al. "Involvement of the AcrAB-TolC Efflux Pump in the Resistance, Fitness, and Virulence of Enterobacter cloacae." Antimicrobial Agents and Chemotherapy 56, no. 4 (2012): 2084–90. http://dx.doi.org/10.1128/aac.05509-11.

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ABSTRACTMultidrug efflux pumps have emerged as important mechanisms of antimicrobial resistance in bacterial pathogens. In order to cause infection, pathogenic bacteria require mechanisms to avoid the effects of host-produced compounds, and express efflux pumps may accomplish this task. In this study, we evaluated the effect of the inactivation of AcrAB-TolC on antimicrobial resistance, fitness, and virulence inEnterobacter cloacae, an opportunistic pathogen usually involved in nosocomial infections. Two different clinical isolates ofE. cloacaewere used, EcDC64 (multidrug resistance overexpres

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Dimitrova, Dobromira I., Rumyana D. Markovska, Temenuga J. Stoeva, et al. "First Report of DHA-1 Producing Enterobacter cloacae Complex Isolate in Bulgaria." Folia Medica 61, no. 3 (2019): 458–61. http://dx.doi.org/10.3897/folmed.61.e39349.

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The aim of the present study was to reveal the characteristics of an Enterobacter cloacae complex isolate producing DHA-1 AmpC enzyme recovered from a patient hospitalized in St Marina Hospital, Varna. Materials and methods: Susceptibility testing, conjugation experiments, isoelectric focusing, PCR and sequencing were carrying out. Results: Of 176 Enterobacter spp. isolates only one isolate was positive for blaDHA. The sequencing revealed the presence of blaDHA-1 and blaCTX-M-3. The antimicrobial susceptibility testing showed higher resistance rates to almost all beta-lactams (ceftazidime, cef

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Clark, Justin, and David Burgess. "Difference in Susceptibility Between Carbapenemase- and Non–Carbapenemase-Producing Carbapenem-Resistant Enterobacteriaceae." Antimicrobial Stewardship & Healthcare Epidemiology 1, S1 (2021): s70. http://dx.doi.org/10.1017/ash.2021.136.

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Background: Carbapenem-resistant Enterobacteriaceae (CRE) remain among the most urgent infectious threats according to the CDC Threats Reports. Although focus has often been placed on carbapenemase-producing phenotypes, there is increasing interest in distinguishing the optimal treatment and outcomes of carbapenemase-producing (CP) and non–carbapenemase-producing (NCP) CRE. We compare antimicrobial susceptibility patterns between CP-CRE and NCP-CRE isolated from patients at our academic medical center. Methods: All CRE isolates of Enterobacter cloacae, Escherichia coli, Klebsiella aerogenes, K

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Sands, Kirsty, Maria J. Carvalho, Edward Portal, et al. "Characterization of antimicrobial-resistant Gram-negative bacteria that cause neonatal sepsis in seven low- and middle-income countries." Nature Microbiology 6, no. 4 (2021): 512–23. http://dx.doi.org/10.1038/s41564-021-00870-7.

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AbstractAntimicrobial resistance in neonatal sepsis is rising, yet mechanisms of resistance that often spread between species via mobile genetic elements, ultimately limiting treatments in low- and middle-income countries (LMICs), are poorly characterized. The Burden of Antibiotic Resistance in Neonates from Developing Societies (BARNARDS) network was initiated to characterize the cause and burden of antimicrobial resistance in neonatal sepsis for seven LMICs in Africa and South Asia. A total of 36,285 neonates were enrolled in the BARNARDS study between November 2015 and December 2017, of who

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Turnidge, John D., Thomas Gottlieb, David H. Mitchell, et al. "Community-onset Gram-negative Surveillance Program annual report, 2012." Communicable Diseases Intelligence 38 (March 1, 2014): 54–58. https://doi.org/10.33321/cdi.2014.38.11.

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The Australian Group on Antimicrobial Resistance performs regular period-prevalence studies to monitor changes in antimicrobial resistance in selected enteric Gram-negative pathogens. The 2012 survey focussed on community-onset infections, examining isolates from urinary tract infections from patients presenting to outpatient clinics, emergency departments or to community practitioners. In 2012, 2,025 Escherichia coli, 538 Klebsiella species and 239 Enterobacter species were tested using a commercial automated method (Vitek 2, BioMérieux) and results were analysed using Clinical and Laboratory

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Jimenez, Alvin, Grignard James Buday, and Rina Opulencia. "Antibiotic Resistome, Mobile Genetic Elements, and Virulence Profile of Multidrug-Resistant Enterobacteriaceae Isolated from Healthy Philippine Native Chicken as Revealed by Analysis of Whole Genome Sequence." Philippine Agricultural Scientist 108, no. 1 (2025): 13–24. https://doi.org/10.62550/bh24010024.

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Gram-negative bacteria, particularly Enterobacteriaceae, acquire antibiotic resistance via horizontal transmission of resistance genes in poultry farms that give rise to multidrug-resistant (MDR) strains. The presence of MDR strains that are potentially pathogenic and zoonotic poses a concern to animal and human health. The aims of this study were to isolate MDR, potentially pathogenic Enterobacteriaceae from Philippine native chicken (PNC) and to analyze the genome of a representative MDR isolate to gain insights into the mechanisms, acquisition, and transmissibility of antibiotic resistance

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Ali, Fatima, Irfan Mirza, Hafsa Waseem, and Humaira Zafar. "The Ever-Worsening Antimicrobial Resistance in Enterobacterales; A wakeup call." Pakistan Armed Forces Medical Journal 73, no. 2 (2023): 468–72. http://dx.doi.org/10.51253/pafmj.v73i2.7832.

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Objective: To determine the comprehensive antibiotic susceptibility profile of carbapenem-resistant Enterobacterales among clinical samples received at the Armed Forces Institute of Pathology (AFIP), Rawalpindi. Study Design: Cross-sectional study. Place and Duration of Study: Department of Microbiology, Armed Forces Institute of Pathology (AFIP), Rawalpindi Pakistan, from Apr to Sep 2020 Methodology: Over six months, 150 carbapenem-resistant Enterobacterales were isolated at the Armed Forces Institute ofPathology, Rawalpindi Pakistan. The antimicrobial susceptibility testing

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Valsesia, Giorgia, Malgorzata Roos, Erik C. Böttger, and Michael Hombach. "A Statistical Approach for Determination of Disk Diffusion-Based Cutoff Values for Systematic Characterization of Wild-Type and Non-Wild-Type Bacterial Populations in Antimicrobial Susceptibility Testing." Journal of Clinical Microbiology 53, no. 6 (2015): 1812–22. http://dx.doi.org/10.1128/jcm.03506-14.

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In this study, we introduce a new approach for determination of epidemiologic cutoffs (ECOFFs) and resistant-population cutoffs (RCOFFs) based on receiver operating characteristic (ROC) curves. As an example, the method was applied for determination of ECOFFs for seven different beta-lactam antibiotics and wild-type populations ofEscherichia coli,Klebsiella pneumoniae, andEnterobacter cloacae. In addition, RCOFFs were determined for bacterial populations with defined resistance mechanisms (“resistotypes”), i.e., extended-spectrum beta-lactamase (ESBL)-positiveE. coli, ESBL-positiveK. pneumonia

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Sangare, Samba Adama, Almoustapha Issiaka Maiga, Ibrehima Guindo, et al. "Prevalence of ESBL-producing Enterobacteriaceae isolated from blood cultures in Mali." Journal of Infection in Developing Countries 10, no. 10 (2016): 1059–64. http://dx.doi.org/10.3855/jidc.7536.

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Introduction: The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL frequency in Enterobacteriaceae isolated from patients’ blood cultures in two university teaching hospitals of Bamako, Mali. Methodology: During a three-month period, the presence of Enterobacteriaceae from blood cultures of patients admitted to the university teaching hospitals of Bamako was evaluated. The microbial identifications were initially performed with an API 20E gallery and VITEK2 locally in Ma

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HARADA, TETSUYA, MASUMI TAGUCHI, RYUJI KAWAHARA, MASASHI KANKI, and KENTARO KAWATSU. "Prevalence and Antimicrobial Susceptibility of Bacterial Pathogens in Ready-to-Eat Foods Retailed in Osaka Prefecture, Japan." Journal of Food Protection 81, no. 9 (2018): 1450–58. http://dx.doi.org/10.4315/0362-028x.jfp-18-035.

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ABSTRACT The potential human health risk of Japanese ready-to-eat (RTE) foods was investigated by determining the contamination by foodborne bacterial pathogens, the prevalence of opportunistic and nosocomial pathogens, and the antibiotic susceptibility of the isolates recovered from 96 samples of lightly pickled vegetables, 88 samples of Western-style desserts, and 98 samples of RTE fish and seafood products sold at retail in Osaka, Japan. Staphylococcus aureus, including isolates producing staphylococcal enterotoxin (SE), were isolated from six lightly pickled vegetable products, seven Weste

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Sedgley, C. M., and L. P. Samaranayake. "Antimicrobial susceptibility of oral isolates of Enterobacter cloacae and Klebsiella pneumoniae from a southern Chinese population." Oral Microbiology and Immunology 13, no. 5 (1998): 315–21. http://dx.doi.org/10.1111/j.1399-302x.1998.tb00713.x.

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Mulinganya, Guy M., Maud Claeys, Serge Z. Balolebwami, et al. "Etiology of Early-Onset Neonatal Sepsis and Antibiotic Resistance in Bukavu, Democratic Republic of the Congo." Clinical Infectious Diseases 73, no. 4 (2021): e976-e980. http://dx.doi.org/10.1093/cid/ciab114.

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Abstract Background The Democratic Republic of the Congo (DRC) has one of the highest neonatal death rates (between 14% and 28%) in the world. In the DRC, neonatal sepsis causes 15.6% of this mortality, but data on the bacterial etiology and associated drug susceptibility are lacking. Methods Hemocultures of 150 neonates with possible early-onset neonatal sepsis (pEOS) were obtained at the Hôpital Provincial Général de Référence de Bukavu (Bukavu, DRC). The newborns with pEOS received an empirical first-line antimicrobial treatment (ampicillin, cefotaxime, and gentamicin) based on the synopsis

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