Gotowe bibliografie tematyczne / ITS-RFLP

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Gotowa bibliografia na temat „ITS-RFLP”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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Artykuły w czasopismach na temat "ITS-RFLP"

Lanoot, Benjamin, Marc Vancanneyt, Bart Hoste, et al. "Grouping of streptomycetes using 16S-ITS RFLP fingerprinting." Research in Microbiology 156, no. 5-6 (2005): 755–62. http://dx.doi.org/10.1016/j.resmic.2005.01.017.

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KOFFI, YAO FULGENCE, CAMELIA DIGUTA, MIREILLE ALLOUE-BORAUD, et al. "PCR-ITS-RFLP identification of pineapple spoilage fungi." Romanian Biotechnological Letters 24, no. 3 (2019): 418–24. http://dx.doi.org/10.25083/rbl/24.3/418.424.

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Midgley, David J., Susan M. Chambers, and John W. G. Cairney. "Spatial distribution of fungal endophyte genotypes in a Woollsia pungens (Ericaceae) root system." Australian Journal of Botany 50, no. 5 (2002): 559. http://dx.doi.org/10.1071/bt02020.

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Fungal endophytes were isolated from hair roots of Woollsia pungens Cav. (Muell.) and mapped according to the root portions from which they were isolated. A total of 119 isolates was obtained and restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer (ITS) region indicated that the isolate assemblage comprised five RFLP types. ITS sequence comparison revealed that RFLP Types I and II had 99.6–99.8% sequence identity with known ericoid mycorrhizal endophytes from Australian epacrids. The remaining three RFLP types were most similar to non-mycorrhizal ascomyc

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Duttweiler, K. B., G. Y. Sun, J. C. Batzer, T. C. Harrington, and M. L. Gleason. "An RFLP-Based Technique for Identifying Fungi in the Sooty Blotch and Flyspeck Complex on Apple." Plant Disease 92, no. 5 (2008): 794–99. http://dx.doi.org/10.1094/pdis-92-5-0794.

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A restriction fragment length polymorphism (RFLP)-based technique was developed to identify members of the sooty blotch and flyspeck (SBFS) disease complex on apple because these fungi are difficult to identify using agar-plate isolation and morphological description. The method includes polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) region of ribosomal DNA (rDNA) using a fungal-specific forward primer (ITS1-F) and an SBFS-specific reverse primer (Myc1-R), followed by digestion of the PCR product by the HaeIII restriction enzyme. When applied to previous

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Hazlianda, Cut, Kamaliah Muis, and Isma Lubis. "A Comparative Study of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism and Fungal Culture for the Evaluation of Fungal Species in Patients with Tinea Cruris." Open Access Macedonian Journal of Medical Sciences 5, no. 7 (2017): 844–47. http://dx.doi.org/10.3889/oamjms.2017.197.

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BACKGROUND: Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophyte infection are slow and less specific. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with the addition of enzyme after amplification, therefore enabling for more specific results.AIM: This study aimed to find whether the PCR-RFLP test could yield the same fungal species result as a fungal culture.METHODS: The specimens were skin scrapings from thirty-one patients suspected tinea cruris

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Sousa, Gabriel S. M., Rodrigo S. De Oliveira, Alex B. De Souza, et al. "Identification of Chromoblastomycosis and Phaeohyphomycosis Agents through ITS-RFLP." Journal of Fungi 10, no. 2 (2024): 159. http://dx.doi.org/10.3390/jof10020159.

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Chromoblastomycosis (CBM) and phaeohyphomycosis (FEO) are infections caused by melanized filamentous fungal agents, primarily found in tropical and subtropical regions. Both infections pose significant challenges for the correct identification of the causative agent due to their morphological similarity, making conventional methods of morphological analysis highly subjective. Therefore, molecular techniques are necessary for the precise determination of these species. In this regard, this study aimed to contribute to a new methodology based on PCR-RFLP for the identification of agents causing

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Wurzburger, Nina, Martin I. Bidartondo, and Caroline S. Bledsoe. "Characterization of Pinus ectomycorrhizas from mixed conifer and pygmy forests using morphotyping and molecular methods." Canadian Journal of Botany 79, no. 10 (2001): 1211–16. http://dx.doi.org/10.1139/b01-079.

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We used morphotyping and molecular methods to characterize ectomycorrhizas of bishop pine (Pinus muricata D. Don) and Bolander pine (Pinus contorta ssp. bolanderi (Parl.) Critchf.) from mixed conifer and hydric pygmy forests on the northern California coast. Sixteen ectomycorrhizal morphotypes were described, producing 15 internal transcribed spacer restriction fragment length polymorphism (ITS-RFLP) types, and 12 were identified via ITS sequencing. From a given site, all root tips of a specific morphotype produced identical ITS-RFLP patterns. However, sometimes two morphotypes produced the sa

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Guillemaut, Cécile, Véronique Edel-Hermann, Pierre Camporota, Claude Alabouvette, Marc Richard-Molard, and Christian Steinberg. "Typing of anastomosis groups ofRhizoctonia solaniby restriction analysis of ribosomal DNA." Canadian Journal of Microbiology 49, no. 9 (2003): 556–68. http://dx.doi.org/10.1139/w03-066.

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A method based on restriction analysis of polymerase chain reaction (PCR)-amplified ribosomal DNA was developed for the rapid characterization of large populations of Rhizoctonia solani at the anastomosis group (AG) level. The restriction maps of the internal transcribed spacers (ITS) sequences were compared for 219 isolates of R. solani belonging to AG-1 to AG-12 and AG-BI, representing diverse geographic and host range origins. Four discriminant restriction enzymes (MseI, AvaII, HincII, and MunI) resolved 40 restriction fragment length polymorphism (RFLP) types among the 219 ITS sequences of

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Burgermeister, Wolfgang, Helen Braasch, Kai Metge, Jianfeng Gu, Thomas Schröder, and Elvira Woldt. "ITS-RFLP analysis, an efficient tool for differentiation of Bursaphelenchus species." Nematology 11, no. 5 (2009): 649–68. http://dx.doi.org/10.1163/156854108/399182.

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Abstract Restriction analysis of amplified ribosomal ITS sequences has provided species-specific fragment patterns for nematodes of several genera, including Bursaphelenchus. We used restriction enzymes RsaI, HaeIII, MspI, HinfI and AluI to produce ITS-RFLP reference profiles of 44 Bursaphelenchus species, including two intraspecific types in each of B. mucronatus and B. leoni. In addition, reference profiles of Aphelenchoides stammeri and Ruehmaphelenchus asiaticus were produced. Reference profiles of six species are shown here for the first time. Identical ITS-RFLP patterns were usually obta

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Said, Halima M., Keshav Krishnamani, Shaheed V. Omar, et al. "Evaluation of Semiautomated IS6110-Based Restriction Fragment Length Polymorphism Typing for Mycobacterium tuberculosis in a High-Burden Setting." Journal of Clinical Microbiology 54, no. 10 (2016): 2547–52. http://dx.doi.org/10.1128/jcm.00408-16.

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The manual IS6110-based restriction fragment length polymorphism (RFLP) typing method is highly discriminatory; however, it is laborious and technically demanding, and data exchange remains a challenge. In an effort to improve IS6110-based RFLP to make it a faster format, DuPont Molecular Diagnostics recently introduced the IS6110-PvuII kit for semiautomated typing ofMycobacterium tuberculosisusing the RiboPrinter microbial characterization system. This study aimed to evaluate the semiautomated RFLP typing against the standard manual method. A total of 112 isolates collected between 2013 and 2

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Rozprawy doktorskie na temat "ITS-RFLP"

Anderson, Ian C., of Western Sydney Nepean University, of Science Engineering and Technology Faculty, and School of Science. "Inter- and intraspecific variation in Pisolithus from central and eastern mainland Australia." THESIS_FST_SS_Anderson_I.xml, 2000. http://handle.uws.edu.au:8081/1959.7/237.

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Pisolithus is an important ectomycorrhizal genus world-wide, however to date we remain largely ignorant of the genetic and functional variation that exists within isolates of this genus. Fifty-three isolates of Pisolithus were obtained from various locations in central and eastern Australia and genetic variation within the isolates was assessed using ITS-RFLP and ITS sequencing analyses. RFLP analysis initially grouped the isolates into eight RFLP types. Neighbour-joining analysis of ITS sequences with Pisolithus ITS sequences available in databases clustered the majority of isolates into four

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Diguta, Filofteia Camelia. "Ecologie des moisissures présentes sur les baies de raisin." Phd thesis, Université de Bourgogne, 2010. http://tel.archives-ouvertes.fr/tel-00597399.

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La microflore des raisins est importante d'un point de vue technologique car elle conditionne en partie la qualité du vin. Or, la diversité des flores fongiques présentes sur baies de raisin ainsi que leur potentiel de contamination du produit final ne sont pas encore pleinement connus. Dans ce cadre, la caractérisation des flores fongiques cultivables présentes sur baies de raisin a été réalisée par PCR ITS-RFLP. 41 espèces de moisissures différentes sur les 43 étudiées appartenant à 11 genres différents ont été caractérisées de façon fiable. Seules les espèces Penicillium thomii et Penicilli

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Keriuscia, Gokul Jarishma. "Eukaryotic diversity of miers valley hypoliths." Thesis, University of the Western Cape, 2012. http://hdl.handle.net/11394/4031.

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>Magister Scientiae - MSc The extreme conditions of Antarctic desert soils render this environment selective towards a diverse range of psychrotrophic microbial communities. Cracks and fissures in translucent quartz rocks permit an adequate amount of penetrating light, sufficient water and nutrients to support cryptic microbial development. Hypolithons colonizing the ventral surface of these quartz rocks have been classified into three types: cyanobacterial dominated (Type I),moss dominated (Type II) and lichenized (Type III) communities. Eukaryotic microbial communities were reported to re

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Diguta, Camelia Filofteia. "Ecologie des moisissures présentes sur les baies de raisin." Thesis, Dijon, 2010. http://www.theses.fr/2010DIJOS045/document.

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La microflore des raisins est importante d’un point de vue technologique car elle conditionne en partie la qualité du vin. Or, la diversité des flores fongiques présentes sur baies de raisin ainsi que leur potentiel de contamination du produit final ne sont pas encore pleinement connus. Dans ce cadre, la caractérisation des flores fongiques cultivables présentes sur baies de raisin a été réalisée par PCR ITS-RFLP. 41 espèces de moisissures différentes sur les 43 étudiées appartenant à 11 genres différents ont été caractérisées de façon fiable. Seules les espèces Penicillium thomii et Penicilli

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Saraiva, Greice Kelle Viegas. "Diversidade genética de leveduras isoladas indústria de leite da Zona da Mata Mineira por RAPD e PCR-RFLP da região ITS do rDNA." Universidade Federal de Viçosa, 2002. http://www.locus.ufv.br/handle/123456789/10643.

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Submitted by Nathália Faria da Silva (nathaliafsilva.ufv@gmail.com) on 2017-06-13T11:57:28Z No. of bitstreams: 1 resumo.pdf: 13320 bytes, checksum: 1edb4e9bce2bdf6b0150e4021e10fe37 (MD5) Made available in DSpace on 2017-06-13T11:57:28Z (GMT). No. of bitstreams: 1 resumo.pdf: 13320 bytes, checksum: 1edb4e9bce2bdf6b0150e4021e10fe37 (MD5) Previous issue date: 2002-11-13 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior A diversidade genética de vinte e sete isolados de leveduras coletadas em laticínios, utilizando como referências dos gêneros Kluyveromyces e Debaryomyces,

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Guérin, Alexis. "Les Lactaires à lait rouge : mycorhization controlée des pins et caractérisation moléculaire : Application à l'étude de la compétence écologique et de la compétitivité d'isolats de lactarius deliciosus." Montpellier, ENSA, 1998. http://www.theses.fr/1998ENSA0004.

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Anderson, Ian C. "Inter- and intraspecific variation in Pisolithus from central and eastern mainland Australia." Thesis, View thesis, 2000. http://hdl.handle.net/1959.7/uws:237.

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Marques, Cálita Pollyanna. "Padronização da análise dos produtos da PCR/RFLP que amplifica os genes Ribossomal Internal transcribed spacer (ITS) e Glucose-6- phosphate dehydrogenase (G6PD) para identificação de Leishmania spp. em gel de poliacrilamida." Universidade Federal de Goiás, 2017. http://repositorio.bc.ufg.br/tede/handle/tede/7789.

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Submitted by Franciele Moreira (francielemoreyra@gmail.com) on 2017-09-21T20:25:51Z No. of bitstreams: 2 Dissertação - Cálita Pollyanna Marques - 2017.pdf: 1468493 bytes, checksum: 90300902d8a48fc5600761dfccf90324 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2017-09-22T11:43:21Z (GMT) No. of bitstreams: 2 Dissertação - Cálita Pollyanna Marques - 2017.pdf: 1468493 bytes, checksum: 90300902d8a48fc5600761dfccf90324 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (M

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Yamoah, Emmanuel. "A model system using insects to vector Fusarium tumidum for biological control of gorse (Ulex europaeus)." Phd thesis, Lincoln University. Bio-Protection and Ecology Division, 2007. http://theses.lincoln.ac.nz/public/adt-NZLIU20080131.114607/.

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The overall objective of this study was to test the hypothesis that insects can vector F. tumidum conidia to infect gorse plants with the aim of developing an alternative approach to mycoherbicide delivery to control weeds. Four potential insect species (Apion ulicis, Cydia ulicetana, Epiphyas postvittana and Sericothrips staphylinus) were assessed for their ability to vector F. tumidum conidia. To achieve this, the external microflora (bacteria and fungi) and the size and location of fungal spores on the cuticle of these insect species were determined. In addition, the ability of the insects

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Kolenda, Magdalena. "The polymorphism in GDF8 gene and its association with meat performance in the chosen sheep breeds." Rozprawa doktorska, Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy, 2016. http://dlibra.utp.edu.pl/Content/937.

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Książki na temat "ITS-RFLP"

Hering, Olaf. Charakterisierung und Differenzierung bei Fusarium Link mittels RAPD und ITS-RFLP. Parey, 1997.

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Części książek na temat "ITS-RFLP"

Rousseaux, Sandrine, and Michèle Guilloux-Bénatier. "PCR ITS-RFLP for Penicillium Species and Other Genera." In Methods in Molecular Biology. Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6707-0_21.

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Croft, J. H., V. Bhattacherjee, and K. E. Chapman. "RFLP Analysis of Nuclear and Mitochondrial DNA and its Use in Aspergillus Systematics." In Modern Concepts in Penicillium and Aspergillus Classification. Springer US, 1990. http://dx.doi.org/10.1007/978-1-4899-3579-3_28.

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Maccaferri, Marco, Martina Bruschi, and Roberto Tuberosa. "Sequence-Based Marker Assisted Selection in Wheat." In Wheat Improvement. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-90673-3_28.

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AbstractWheat improvement has traditionally been conducted by relying on artificial crossing of suitable parental lines followed by selection of the best genetic combinations. At the same time wheat genetic resources have been characterized and exploited with the aim of continuously improving target traits. Over this solid framework, innovations from emerging research disciplines have been progressively added over time: cytogenetics, quantitative genetics, chromosome engineering, mutagenesis, molecular biology and, most recently, comparative, structural, and functional genomics with all the re

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Beyhan, Yunus Emre. "Cystic Echinococcosis and Molecular Diagnosis." In Molecular Approaches in Medicine. Nobel Tip Kitabevleri, 2024. http://dx.doi.org/10.69860/nobel.9786053359524.1.

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Cystic echinococcosis (CE) is a parasitic disease caused by the larval form of Echinococcus granulosus. It predominantly affects the liver but can also impact the lungs, kidneys, spleen, brain, bones, and heart. CE is prevalent in regions with low socio-economic status and is associated with significant health and economic burdens due to medical costs and reduced livestock productivity. Diagnosis typically involves radiological and serological methods, and treatment primarily consists of surgery, though drug therapy and less invasive procedures like PAIR are also used. Prevention focuses on co

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Krause, D., R. Szibor, W. Kuchheuser, and R. Brückner. "The Practical Significance of Human Genetic RFLP-Systems in Paternity Testing." In DNA — Technology and Its Forensic Application. Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76632-9_20.

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Scheithauer, R., and H. J. Weisser. "DNA Extraction and RFLP Analysis of Bloodstains on a Variety of Textiles — Investigation of Various Extraction Procedures." In DNA — Technology and Its Forensic Application. Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76632-9_26.

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Horváth László and Rudas Imre J. "Product Behavior Definition for Element Generation in RFLP Structure." In Frontiers in Artificial Intelligence and Applications. IOS Press, 2015. https://doi.org/10.3233/978-1-61499-522-7-485.

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Lifecycle management of product information is a recent paradigm. It is essential at knowledge driven integration of model generation processes in virtual engineering. In the centre of virtual engineering environment complex product model includes representations for lifecycle of product. Recently, generic product models apply built in active knowledge at self generation of their instances. Widespread development of multidisciplinary products generated demand for higher level abstraction than representation of physically existing objects in product model. For this purpose, product models in le

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Horváth László and Rudas Imre J. "New Method for Generation of RFLP Structure Elements in PLM Model." In Frontiers in Artificial Intelligence and Applications. IOS Press, 2014. https://doi.org/10.3233/978-1-61499-434-3-310.

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Engineering modeling for lifecycle management of product information is challenged with the new demand for representation of multidisciplinary product in a single complex model system. Leading developers of product lifecycle management (PLM) systems recognized the need for conceptual modeling of industrial products in higher abstraction level than the classical physical level representation of product and related objects. They turned to PLM models which use representation of product in the requirement, functional, logical, and physical (RFLP) structure. This means introduction of systems engin

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Bouayed Abdelmoula, Nouha, Samir Aloulou, Sonda Kammoun, et al. "PCR assay using 22q11.2 highly polymorphic markers for exclusion of 22q microdeletion: Technical optimization and application in North Africa." In Advances in Genetic Polymorphisms [Working Title]. IntechOpen, 2023. http://dx.doi.org/10.5772/intechopen.1002331.

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22q11.2 deletion syndrome is a genomic disorder with a broader clinical and genetic spectrum. To exclude the presence of 22q11.2 microdeletion, we optimize a PCR-RFLP analysis of three SNP located in the typically proximal 22q11.21 deleted region of 1.5 Mb. PCR reactions, optimized with a Touch-Down program, were performed using three pairs of primers. The amplicons were cleaved by three restrictive enzymes: HaeIII, CviAII, and BsrI applied respectively, for rs4819523, rs4680, and rs5748411. The efficiency of this PCR RFLP assay was confirmed in the light of its application in a small cohort o

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Erlich, Henry A. "Exclusions and Exonerations." In Genetic Reconstruction of the Past. Oxford University Press, 2023. http://dx.doi.org/10.1093/oso/9780197675366.003.0004.

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Abstract This chapter describes the first DNA exoneration, in which the wrongful conviction of Gary Dotson was overturned based on the results of the PCR-based HLA DQalpha test. Attempts to analyze the 11-year-old semen stain using restriction fragment length polymorphism (RFLP) technology had been unsuccessful. The author discusses several other HLA-DQalpha–based exonerations as well as many more recent cases using more recent DNA technology, the short tandem repeat (STR) markers. This chapter addresses the issues of mistaken eyewitness testimony, false confessions, and the reluctance of pros

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Streszczenia konferencji na temat "ITS-RFLP"

Sharma, Vineeta, Pallavi Singhal, Anoop Kumar, V. G. Ramachandran, Shukla Das та Mausumi Bharadwaj. "Association of TNF-α–rs 281865419 polymorphism with reproductive tract infections in Indian population". У 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685357.

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Aim: To investigate the presence of reproductive tract infections (RTIs) in symptomatic and asymptomatic women in North India and association of SNPs in TNF? gene (rs-281865419 C/T) with susceptibility to these RTIs. Methods: We collected 100 symptomatic (cases) and 100 asymptomatic women (controls) samples and screened them for RTIs. Then genotyping of TNF-? gene was performed by PCR-RFLP. Results: Among cases the frequencies of RTIs infection is higher than control. The prevalence of HPV, C. trachomatis, T. vaginalis, Bacterial vaginosis and N. gonorrhoeae are 28% and 6%; 11%, 32% respective

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Sharma, Vineeta, Pallavi Singhal, Anoop Kumar, V. G. Ramachandran, Shukla Das та Mausumi Bharadwaj. "Association of TNF-α rs-281865419 polymorphism with reproductive tract infections in Indian population". У 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685270.

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Aim: To investigate the presence of reproductive tract infections (RTIs) in symptomatic and asymptomatic women in North India and association of SNPs in TNFα gene (rs-281865419 C/T) with susceptibility to these RTIs. Methods: We collected 100 symptomatic (cases) and 100 asymptomatic women (controls) samples and screened them for RTIs. Then genotyping of TNF-α gene was performed by PCR-RFLP. Results: Among cases the frequencies of RTIs infection is higher than control. The prevalence of HPV, C. trachomatis, T. vaginalis, Bacterial vaginosis and N. gonorrhoeae are 28% & 6%; 11%, 32% respecti

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Raporty organizacyjne na temat "ITS-RFLP"

Dubcovsky, Jorge, Tzion Fahima, and Ann Blechl. Positional cloning of a gene responsible for high grain protein content in tetraploid wheat. United States Department of Agriculture, 2003. http://dx.doi.org/10.32747/2003.7695875.bard.

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High Grain Protein Content (GPC) is a desirable trait in breadmaking and pasta wheat varieties because of its positive effects on quality and nutritional value. However, selection for GPC is limited by our poor understanding of the genes involved in the accumulation of protein in the grain. The long-term goal of this project is to provide a better understanding of the genes controlling GPC in wheat. The specific objectives of this project were: a) to develop a high-density genetic map of the GPC gene in tetraploid wheat, b) to construct a T. turgidum Bacterial Artificial Chromosome (BAC) libra

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Feldman, Moshe, Eitan Millet, Calvin O. Qualset, and Patrick E. McGuire. Mapping and Tagging by DNA Markers of Wild Emmer Alleles that Improve Quantitative Traits in Common Wheat. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7573081.bard.

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The general goal was to identify, map, and tag, with DNA markers, segments of chromosomes of a wild species (wild emmer wheat, the progenitor of cultivated wheat) determining the number, chromosomal locations, interactions, and effects of genes that control quantitative traits when transferred to a cultivated plant (bread wheat). Slight modifications were introduced and not all objectives could be completed within the human and financial resources available, as noted with the specific objectives listed below: 1. To identify the genetic contribution of each of the available wild emmer chromosom

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Wongpiyabovorn, Jongkonnee, Nattiya Hirankarn, Yingyos Avihingsanon, Tewin Tencomnao, Yong Poovorawan, and Kriangsak Ruchusatsawat. The association between immunogenetics and genetic susceptibility of psoriasis in Thai population. Chulalongkorn University, 2006. https://doi.org/10.58837/chula.res.2006.27.

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Psoriasis is T-cell-mediated skin autoimmunity, required environmental triggers and genetic susceptibility factors to become manifested. Psoriasis is a chronic skin disease characterized by the abnormal hyperproliferation and differentiation of the epidermis, elongated and prominent blood vessels and a thick perivascular lymphocytic infiltrate. Vascular endothelial growth factor (VEGF) gene play important role in pathogenesis of various diseases with angiogenic basis such as breast cancer and autoimmune disease including psoriasis. Many studies analyzed the association of VEGF gene polymorphis

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