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1

Setiarto, Raden Haryo Bimo, Nunuk Widhyastuti, and Nimas Ayu Rikmawati. "Optimasi Konsentrasi Fruktooligosakarida untuk Meningkatkan Pertumbuhan Bakteri Asam Laktat Starter Yoghurt (CONCENTRATION OPTIMIZATION OF FRUCTOOLIGOSACCHARIDES TO INCREASE GROWTH OF LACTIC ACID BACTERIA YOGHURT STARTER)." Jurnal Veteriner 18, no. 3 (2017): 428. http://dx.doi.org/10.19087/jveteriner.2017.18.3.428.

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Fructooligosaccharides are prebiotic source that widely used in food products, such as: fermented milk and infant formula. Prebiotics are food components that cannot be digested in the digestive tract enzymatically. However, they can be fermented by probiotic bacteria in the colon. This study aimed to determine the optimum concentrations of fructooligosaccharides in order to increase the growth of lactic acid bacteria yogurt starter (Lactobacillus acidophillus, Lactobacillus bulgaricus, Streptococcus thermophillus). Optimation concentration of fructooligosaccharides on the growth of Lactobacil
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Agustono, B., R. Apriliawati, A. E. wijaya, et al. "Inhibition of Lactobacillus acidophillus, Lactobacillus, Bacteria plantarum and Bifidobacterium spp. against Escherichia coli and Salmonella sp. in the digestive tract in vitro." IOP Conference Series: Earth and Environmental Science 1273, no. 1 (2023): 012022. http://dx.doi.org/10.1088/1755-1315/1273/1/012022.

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Abstract The bacteria Lactobacillus acidophillus, Lactobacillus plantarum and Bifidobacterium spp. is a lactic acid bacteria that is used as a probiotic. The research was conducted with the aim of knowing the inhibition of the pribiotic bacteria Lactobacillus acidophillus, Lactobacillus plantarum and Bifidobacterium spp. against Escherichia coli and Salmonella sp. Supporting tests such as motility tests, catalase tests, and fermentation tests were also carried out. The media used in this study were: Mueller Hinton Agar (MHA) with the Kirby-Bauer method and Nutrient Broth (NB) media, TSIA, SIM.
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Guan, Le Luo, Karen E. Hagen, Gerald W. Tannock, Doug R. Korver, Gaylene M. Fasenko, and Gwen E. Allison. "Detection and Identification of Lactobacillus Species in Crops of Broilers of Different Ages by Using PCR-Denaturing Gradient Gel Electrophoresis and Amplified Ribosomal DNA Restriction Analysis." Applied and Environmental Microbiology 69, no. 11 (2003): 6750–57. http://dx.doi.org/10.1128/aem.69.11.6750-6757.2003.

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ABSTRACT The microflora of the crop was investigated throughout the broiler production period (0 to 42 days) using PCR combined with denaturing gradient gel electrophoresis (PCR-DGGE) and selective bacteriological culture of lactobacilli followed by amplified ribosomal DNA restriction analysis (ARDRA). The birds were raised under conditions similar to those used in commercial broiler production. Lactobacilli predominated and attained populations of 108 to 109 CFU per gram of crop contents. Many of the lactobacilli present in the crop (61.9% of isolates) belonged to species of the Lactobacillus
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DRAKE, MARYANNE, CHRISTOPHER L. SMALL, KEMET D. SPENCE, and BARRY G. SWANSON. "Rapid Detection and Identification of Lactobacillus spp. in Dairy Products by Using the Polymerase Chain Reaction." Journal of Food Protection 59, no. 10 (1996): 1031–36. http://dx.doi.org/10.4315/0362-028x-59.10.1031.

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Species-specific primers for use in the polymerase chain reaction (PCR) were designed to differentially amplify DNA from the common dairy lactobacillus species Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus helveticus, and Lactobacillus acidophilus. A method for rapid extraction of bacterial DNA from dairy products was developed. The sensitivity of bacterial DNA extraction from food and subsequent amplification by PCR was 100 cells total. Lactobacillus DNA was extracted and identified from commercial yoghurts, acidophilus milk, and cheeses. The methodology allows the presumptive
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Kukhtyn, M., V. Salata, O. Hudym, I. Masniak, O. Kokovskiy, and Т. Ganushchyn. "Assessment of probiotic properties of lactic acid bacteria isolated from dairy products." Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies 27, no. 103 (2025): 15–22. https://doi.org/10.32718/nvlvet-f10303.

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The rapid development of “fast-cooked food” due to industrialization of food production leads to a decrease in health. Therefore, food consumption that, in addition to nutritional function, helps to improve digestive processes is currently very relevant. As a result, the demand for foods that contain probiotics is growing worldwide due to the constant accumulation of results that indicate their potential benefit to consumers' health. The purpose of the work was to determine the probiotic properties of lactic acid bacteria with improving the recipe of dairy drink by introducing Lactobacillus ac
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Рябцева, Светлана, Svetlana Ryabtseva, Валида Ахмедова, Valida Akhmedova, Георгий Анисимов, and Georgiy Anisimov. "Ice cream as a carrier of Lactobacillus acidophilus." Food Processing: Techniques and Technology 48, no. 2 (2019): 5–27. http://dx.doi.org/10.21603/2074-9414-2018-2-5-27.

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Ice cream is a product with specific composition and properties that are highly valued by a wide range of consumers. Peculiarities of ice cream composition and production technology make it possible to consider the product as a promising carrier and means of biologically active compounds and useful microorganisms supply. The article reveals morphological, biochemical, physiological, genetic and technological characteristics of L. acidophilus. It systematizes information on the existing methods used for production of ice cream with L. acidophilus. The author shows the influence of various forms
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Boyarineva, I. V. "PROBIOTIC PROPERTIES AND RELEVANCE OF PRACTICAL APPLICATION L. ACIDOPHILUS." Vestnik of Khabarovsk State University of Economics and Law, no. 2 (106) (August 5, 2021): 70–75. http://dx.doi.org/10.38161/2618-9526-2021-2-70-75.

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Creating probiotic complex starter cultures and increasing the probiotic activity of traditional dairy products is an urgent task today. The active principle of these products is live lactobacilli, in particular, L. Acidophilus, which have a wide range of probiotic activity. The article is devoted to the description of the probiotic culture of Lactobacillus acidophilus. The article describes the probiotic and biochemical properties of Lactobacillus Acidophilus, provides examples of the practical use of this culture in the medical and food industries.
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Horáčková, Š., P. Sedláčková, M. Sluková, and M. Plocková. "The influence of whey, whey component and malt on the growth and acids production of lactobacilli in milk." Czech Journal of Food Sciences 32, No. 6 (2014): 526–31. http://dx.doi.org/10.17221/214/2014-cjfs.

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The effect of whey powder, whey protein concentrate, caseinomacropeptide, and malt addition into milk on the growth and acid production of lactobacilli (Lactobacillus casei Lafti L-26, Lactobacillus acidophilus CCDM 151, and Lactobacillus casei CCDM 198) was evaluated. The ability of these strains to use different types of saccharides from milk and plant sources was also tested. Glucose, galactose, fructose and maltose were utilised by all tested strains. The results showed that the addition of malt positively affected the growth of lactobacilli strains compared to the growth in milk enriched
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Journal, Baghdad Science. "Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells." Baghdad Science Journal 8, no. 3 (2011): 723–27. http://dx.doi.org/10.21123/bsj.8.3.723-727.

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The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fer
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Al-Mathkhury, Harith J. F., and Alaa' Mohammed Hasan. "Effect of Lactobacilli sources on Escherichia coli and Staphylococcus aureus adherence to uroepithelial cells." Baghdad Science Journal 8, no. 3 (2011): 723–27. http://dx.doi.org/10.21123/bsj.2011.8.3.723-727.

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The inhibitory action of four lactobacilli isolates Lactobacillus bulgaricus, L. acidophilus, L. plantarum and L. fermentum, isolated from four different samples; yoghurt, vinegar, saliva and vagina respectively, on Escherichia coli and Staphylococcus aureus adhesion to uroepithelial cells were investigated. Results showed that all Lactobacillus isolates or their supernatant were able to reduce the number of the uropathogens attached to uroepithelial cells. However, inhibition level of lactobacilli cells was higher than their supernatant. Nevertheless, the human indigenous lactobacilli (L. fer
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Khudhir, Zina Saab. "The synergistic effects of Lactobacillus acidophillus ROO52 and Lactobacillus bulgaricus LB-12 bacteriocins against E.coli O157 :H7 in milk." Iraqi Journal of Veterinary Medicine 38, no. 2 (2014): 35–40. http://dx.doi.org/10.30539/iraqijvm.v38i2.220.

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The aims of this study to evaluate the antibacterial potential of standard strains (Lactobacillus acidophilus ROO52 and Lactobacillus bulgaricus LB-12) that produce bacteriocins against E.coli O157:H7 in raw milk. Thirty raw milk samples randomly were collected weekly different markets of Baghdad city in order to investigate the prevalence of E.coli O157:H7 in milk. The prevalence of E.coli O157:H7 in raw milk samples was 6 out 30 (20%) by using the modern chromogenic media with serological latex agglutination test kit. The average diameter of the inhibition zone of crude bacteriocin against E
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Solehah, Mar’atus, RR Riyanti, Veronica Wanniatie, and Dian Septinova. "PENGARUH PEMBERIAN Lactobacillus acidophilus TERHADAP pH DAN DAYA IKAT AIR DAGING BROILER." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 6, no. 2 (2022): 151–57. http://dx.doi.org/10.23960/jrip.2022.6.2.151-157.

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This research aimed to (1) determine the effect of giving Lactobacillus acidophilus on pH and water holding capacity of broiler meat and (2) to determine the best concentration of Lactobacillus acidophilus on pH and water holding capacity of broiler meat. This research was carried out in September 2021 at the Animal Production Laboratory of the Animal Husbandry Department, University of Lampung and the Agricultural Product Technology Laboratory of Food Technology Department of the Lampung State Polytechnic. The research material used were 20 broiler chicken breast fillets. This study used a co
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Oh, Sejong. "Lactobacillus acidophilus as a Probiotics." Journal of Milk Science and Biotechnology 37, no. 3 (2019): 155–66. http://dx.doi.org/10.22424/jmsb.2019.37.3.155.

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Tan, Rachael, and Wai Mun Loke. "Gut Oxidative Modulation of Polyphenol, Prebiotic, Probiotic, and Postbiotic in Vitro." Current Research in Nutrition and Food Science Journal 10, no. 1 (2022): 56–70. http://dx.doi.org/10.12944/crnfsj.10.1.05.

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The study evaluated the individual and combined influence of polyphenol (quercetin), prebiotic (galactooligosaccharide), probiotic ( Lactobacillus acidophilus), and/ or postbiotic (inanimate Lactobacillus acidophilus) on the cellular oxidative status of CACO-2 intestinal epithelial cells. The CACO-2 cells were treated with quercetin (1 µmol L-1), galactooligosaccharide (4 mg mL-1), Lactobacillus acidophilus (2 x 106 CFU mL-1), and/or inanimate Lactobacillus acidophilus (2 x 106 CFU mL-1) individually and in all combinations over twenty four hours. Cellular antioxidant capacities (DPPH radical
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Cheng, Haixin, Yi Ma, Xiaohui Liu, Chao Tian, Xuli Zhong, and Libo Zhao. "A Systematic Review and Meta-Analysis: Lactobacillus acidophilus for Treating Acute Gastroenteritis in Children." Nutrients 14, no. 3 (2022): 682. http://dx.doi.org/10.3390/nu14030682.

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The efficacy of probiotic strains of Lactobacillus acidophilus to manage acute gastroenteritis in children is still not established. We searched the Cochrane Library, PubMed, EMBASE, and three Chinese literature databases (CNKI, WanFang, and CBM) from their inception to February 2021 for RCTs that compared the use of Lactobacillus acidophilus with no Lactobacillus acidophilus. The grey literature was searched through Google Scholar. Authors of the original papers were contacted for additional data. The study included a total of 15 RCTs involving 1765 patients. Compared with placebo or no treat
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Irkitova, A. N., та A. V. Matsyura. "Эколого-биологическая характеристика Lactobacillus acidophilus". Ukrainian Journal of Ecology 7, № 4 (2017): 214–30. http://dx.doi.org/10.15421/2017_109.

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<p>Lactobacillus acidophilus - homofermentative lactobacillus, specializing in living in the gastrointestinal and urogenital tracts of mammals and birds. It accompanies a person from birth and throughout his life, providing a whole range of useful services, the main one of which is active participation in the body's defense system against the harmful action of undesirable microorganisms (preventing the growth of pathogenic bacteria and restraining populations of opportunistic microbes at a safe level) . It is this property of the acidophilus rod that explains its wide practical use in va
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Boris, Soledad, Juan E. Suárez, Fernando Vázquez, and Covadonga Barbés. "Adherence of Human Vaginal Lactobacilli to Vaginal Epithelial Cells and Interaction with Uropathogens." Infection and Immunity 66, no. 5 (1998): 1985–89. http://dx.doi.org/10.1128/iai.66.5.1985-1989.1998.

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ABSTRACT Three strains of Lactobacillus, identified asLactobacillus acidophilus, Lactobacillus gasseri, and Lactobacillus jensenii, were selected from among 70 isolates from the vaginas of healthy premenopausal women for properties relevant to mucosal colonization or antagonism. All three self-aggregated and adhered to epithelial vaginal cells, displacing well-known vaginal pathogens, such as G. vaginalis, and inhibiting the growth in vitro ofEscherichia coli and Streptococcus agalactiae. The surface components involved in self-aggregation appeared to be proteins for L. gasseri and lipoprotein
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Berger, Bernard, R. David Pridmore, Caroline Barretto, et al. "Similarity and Differences in the Lactobacillus acidophilus Group Identified by Polyphasic Analysis and Comparative Genomics." Journal of Bacteriology 189, no. 4 (2006): 1311–21. http://dx.doi.org/10.1128/jb.01393-06.

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ABSTRACT A set of lactobacilli were investigated by polyphasic analysis. Multilocus sequence analysis, DNA typing, microarray analysis, and in silico whole-genome alignments provided a remarkably consistent pattern of similarity within the Lactobacillus acidophilus complex. On microarray analysis, 17 and 5% of the genes from Lactobacillus johnsonii strain NCC533 represented variable and strain-specific genes, respectively, when tested against four independent isolates of L. johnsonii. When projected on the NCC533 genome map, about 10 large clusters of variable genes were identified, and they w
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Bhattacharyya, Barun K., S. Chowdhury, S. Das, et al. "Selective Enumeration of Lactobacillus acidophilus for Probiotic Formulations." International Journal of Pharmaceutical Sciences and Nanotechnology 7, no. 4 (2014): 2646–50. http://dx.doi.org/10.37285/ijpsn.2014.7.4.6.

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Probiotic microorganisms have been utilized for many years in food processing, preservations and for nutraceutical health benefits. There are a large number of pharmaceutical formulations containing probiotic microorganisms available in the market worldwide. Majority of these organisms are the members specifically from genera Lactobacillus, Lactococcus and Bifidobacterium. A number of media have been proposed for selective or differential enumeration of lactobacilli and bifidobacteria in mixed populations. The development of selective medium using different types of ingredients and antibiotic
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Bruno-Bárcena, Jose M., Jason M. Andrus, Stephen L. Libby, Todd R. Klaenhammer, and Hosni M. Hassan. "Expression of a Heterologous Manganese Superoxide Dismutase Gene in Intestinal Lactobacilli Provides Protection against Hydrogen Peroxide Toxicity." Applied and Environmental Microbiology 70, no. 8 (2004): 4702–10. http://dx.doi.org/10.1128/aem.70.8.4702-4710.2004.

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ABSTRACT In living organisms, exposure to oxygen provokes oxidative stress. A widespread mechanism for protection against oxidative stress is provided by the antioxidant enzymes: superoxide dismutases (SODs) and hydroperoxidases. Generally, these enzymes are not present in Lactobacillus spp. In this study, we examined the potential advantages of providing a heterologous SOD to some of the intestinal lactobacilli. Thus, the gene encoding the manganese-containing SOD (sodA) was cloned from Streptococcus thermophilus AO54 and expressed in four intestinal lactobacilli. A 1.2-kb PCR product contain
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Beekwilder, Jules, Daniela Marcozzi, Samuele Vecchi, et al. "Characterization of Rhamnosidases from Lactobacillus plantarum and Lactobacillus acidophilus." Applied and Environmental Microbiology 75, no. 11 (2009): 3447–54. http://dx.doi.org/10.1128/aem.02675-08.

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ABSTRACT Lactobacilli are known to use plant materials as a food source. Many such materials are rich in rhamnose-containing polyphenols, and thus it can be anticipated that lactobacilli will contain rhamnosidases. Therefore, genome sequences of food-grade lactobacilli were screened for putative rhamnosidases. In the genome of Lactobacillus plantarum, two putative rhamnosidase genes (ram1 Lp and ram2 Lp) were identified, while in Lactobacillus acidophilus, one rhamnosidase gene was found (ramA La). Gene products from all three genes were produced after introduction into Escherichia coli and we
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Halim, Susanna, Florenly Florenly, and Shely Anggriani. "Uji Efektivitas Antibakteri Ekstrak Kulit Buah Delima Merah (Punica granatum L.) terhadap Pertumbuhan Lactobacillus acidophilus Secara In Vitro." e-GiGi 11, no. 2 (2023): 318–25. http://dx.doi.org/10.35790/eg.v11i2.46515.

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Abstract: Lactobacillus acidophilus is a Gram-positive bacterium that causes caries. It is known that natural ingredients of red pomegranate peel extract (Punica granatum L.) have antibacterial effect. This study aimed to determine the effectiveness of red pomegranate peel extract in inhibiting the growth of Lactobacillus acidophilus. This was a laboratory and experimental study using a post-test only design with a control group design. Samples were Lactobacillus acidophilus bacteria. Sample size was determined using the Federer's formula, and five repetitions were obtained for each group. Dat
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Chen, He, Li Yuan Ji, Guo Wei Shu, and Zhao Wei Wang. "Effect of Lithium Chloride and Sodium Propionate on Growth of Selected Probiotics." Key Engineering Materials 480-481 (June 2011): 66–69. http://dx.doi.org/10.4028/www.scientific.net/kem.480-481.66.

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Effect of Lithium Chloride (LiCl) and Sodium Propionate (CH3CH2COONa) on growth of Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus bulgaricus, Lactobacillus casei and Lactobacillus rhamnosus was studied by measuring optical density at 600nm (OD600) and pH using MRS media as the control. The addition of each substance (%, w/v) was 0.025%, 0.05%, 0.1%, 0.2% and 0.3%. Results were as follows: addition of LiCl has the significant inhibition on growth of Lactobacillus acidophilus, Bifidobacterium bifidum and Lactobacillus bulgaricus at incubation 12h. The optimum selective concent
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Кожахметова, З. А., Р. Ж. Мыктыбаева, Г. Б. Аталихова, А. Р. Сансызбай та Ж. М. Утебаев. "Лактозосбраживающая способность молочнокислых бактерий выделенные из природных источников". Ġylym ža̋ne bìlìm 1, № 4 (77) (2024): 3–13. https://doi.org/10.52578/2305-9397-2024-4-1-3-13.

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Исследовано влияние различных концентраций лактозы (3, 4, 5, 6, 7, 8) на кислотообразование выделенных новых штаммов молочнокислых бактерий Lactobacillus casei, Lactobacillus bulgaricus, Lactobacillus acidophilus, значимых при производстве пробиотических препаратов. Экспериментально обоснована наиболее эффективная композиция стартовых культур с учетом специфики вырабатываемого цельномолочного продукта, состоящая из Lactobacillus bulgaricus, Lactobacillus acidophilus. Установлено, что оптимальными условиями, позволяющими достичь наивысшей метаболической активности молочнокислыми бактериями в ср
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Denkova, Zapryana, Velichka Yanakieva, Bogdan Goranov, et al. "In vitro examination of the antimicrobial activity of a potentially probiotic Lactobacillus acidophilus strain against Escherichia coli, Staphylococcus aureus and Salmonella sp." BIO Web of Conferences 45 (2022): 02010. http://dx.doi.org/10.1051/bioconf/20224502010.

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An interesting property for many probiotic strains is to exhibit antimicrobial activity against pathogenic microorganisms. Therefore, the antimicrobial activity of the potentially probiotic strain Lactobacillus acidophilus L12 against Escherichia coli, Staphylococcus aureus and Salmonella sp. was examined in vitro through co-culturing of Lactobacillus acidophilus L12 with each of the pathogenic strains for 72 h. When cultured individually, all strains reached a final population always exceeding 1012 cfu/mL. When co-cultured with each of the pathogenic strains Lactobacillus acidophilus L12 popu
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Arzakiyah, Fina, Veronica Wanniatie, Ali Husni, and Arif Qisthon. "TOTAL ASAM, KEASAMAN, DAN VISKOSITAS YOGHURT SUSU SAPI DENGAN MENGGUNAKAN KOMBINASI STARTER YANG BERBEDA." Jurnal Riset dan Inovasi Peternakan (Journal of Research and Innovation of Animals) 8, no. 1 (2024): 150–56. http://dx.doi.org/10.23960/jrip.2024.8.1.150-156.

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Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi starter bakteri terhadap kualitas fisik yoghurt susu sapi yang meliputi total asam, keasaman dan viskositas. Penelitian ini dilaksanakan pada Januari 2023 di Laboratorium Produksi Ternak, Jurusan Peternakan Fakultas Pertanian Universitas Lampung dan pengujian sampel dilakukan di Laboratorium Teknologi Hasil Pertanian Politeknik Negeri Lampung. Penelitian ini dilakukan menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan yaitu P1 (Streptococcus thermophilus + Lactobacillus bulgaricus); P2 (Streptococcus thermophilus + Lactobaci
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Budilovskaya, Olga V., Elena V. Spasibova, Kira V. Shalepo, et al. "Antagonistic and antibacterial activity of <i>Lactobacillus rhamnosus</i> HN001 and <i>Lactobacillus acidophilus</i> La-14 included in the oral probiotic." Journal of obstetrics and women's diseases 73, no. 3 (2024): 27–39. http://dx.doi.org/10.17816/jowd630698.

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BACKGROUND: A microbial imbalance in the vaginal biotope leads to the development of vaginal infections and is associated with urinary tract infections. The ineffectiveness of the therapy and the frequent recurrence of these infections are important reasons for searching for alternative treatment strategies. A progressive solution to this issue has been the use of oral probiotics with a targeted effect on the intestinal and vaginal microflora. AIM: The aim of this study was to evaluate the antagonistic and antibacterial activity of the probiotic strains Lactobacillus rhamnosus HN001 and Lactob
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Klein, Natalie, Paul E. Schoch, and Burke A. Cunha. "Lactobacillus acidophilus liver abscess." Infectious Diseases Newsletter 10, no. 12 (1991): 101–2. http://dx.doi.org/10.1016/0278-2316(91)90030-4.

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King, V. A. E., and J. T. Su. "Dehydration of Lactobacillus acidophilus." Process Biochemistry 28, no. 1 (1993): 47–52. http://dx.doi.org/10.1016/0032-9592(94)80035-9.

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Kabir, SM Lutful, SM Mushfiqur Rahman, Sucharit Basu Neogi, Mohummad Muklesur Rahman, and M. Shahidur Rahman Khan. "Isolation, identification, molecular characterization and screening of probiotic activities of Lactobacillus species from poultry sources at live bird markets in Mymensingh, Bangladesh." Asian-Australasian Journal of Bioscience and Biotechnology 1, no. 1 (2016): 54–65. http://dx.doi.org/10.3329/aajbb.v1i1.61531.

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A study was conducted to isolate and characterize Lactobacilli from the gastrointestinal (GI) tract of broiler chickens in different markets along with evaluation of probiotic ability and antibiotic sensitivity of Lactobacillus isolates during the period of July, 2014 to December, 2014. The caecum and cloacal contents of 100 broiler chickens from different markets were collected and cultured on Man Rogosa and Sharp (MRS) broth and agar. Lactobacilli were isolated and characterized by using phenotypic (cell morphology, Gram’s staining, physiological and biochemical tests which are specific for
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31

Hütt, P., P. Kõll, J. Stsepetova, et al. "Safety and persistence of orally administered human Lactobacillus sp. strains in healthy adults." Beneficial Microbes 2, no. 1 (2011): 79–90. http://dx.doi.org/10.3920/bm2010.0023.

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The aim of the study was to evaluate the safety and persistence of selected Lactobacillus strains in the gastrointestinal tract (GIT) of healthy adult volunteers after oral consumption of high doses of lactobacilli to identify potential candidates for probiotic and biotechnological applications. In the first phase of the study, nine individuals consumed capsules containing Lactobacillus gasseri 177 and E16B7, Lactobacillus acidophilus 821-3, Lactobacillus paracasei 317 and Lactobacillus fermentum 338-1-1 (each daily dose 1×1010 cfu) for 5 consecutive days. Data on gut health, blood parameters,
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Haarman, Monique, and Jan Knol. "Quantitative Real-Time PCR Analysis of Fecal Lactobacillus Species in Infants Receiving a Prebiotic Infant Formula." Applied and Environmental Microbiology 72, no. 4 (2006): 2359–65. http://dx.doi.org/10.1128/aem.72.4.2359-2365.2006.

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ABSTRACT The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. However, the Lactobacillus species distribution in breast-fed or formula-fed infants has not yet been determined in great detail. For accurate enumeration of different lactobacilli, duplex 5′ nuclease assays, t
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Rahmawati, Siti, Astriana Napirah, Fitrianingsih Fitrianingsih, and Asma Bio Kimestri. "Karakteristik Organoleptik Yogurt Susu Ultra High Temperature (UHT) dengan Penambahan Bakteri Lactobacillus acidophilus." Jurnal Ilmiah Peternakan Halu Oleo 5, no. 3 (2023): 240. http://dx.doi.org/10.56625/jipho.v5i3.40172.

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Mengkonsumsi susu segar secara rutin akan sangat membantu dalam kesehatan tubuh manusia, karena kandungan nutrisi yang terkandung di dalamnya masih bersifat alami. Salah satu upaya penganekaragaman olahan susu UHT adalah yogurt. Yogurt berbahan dasar susu UHT dapat mengurangi resiko kontaminasi karena proses sterilisasi. Penelitian ini bertujuan untuk mengevaluasi karakteristik organoleptik yogurt susu Ultra High Temperature (UHT) dengan penambahan bakteri Lactobacillus acidophilus. Metode penelitian yaitu metode eksperimen. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak l
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Dang Thi, Huong, Trung Nguyen Thanh, Trong Pham Nhu, Ba Tran Hong, and Hong Hao Le Thi. "Development of the real-time PCR method for detecting lactobacillus acidophillus in some functional foods matrices." Heavy metals and arsenic concentrations in water, agricultural soil, and rice in Ngan Son district, Bac Kan province, Vietnam 2, no. 2 (2019): 2–7. http://dx.doi.org/10.47866/2615-9252/vjfc.69.

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In the past decades, Lactobacillus species have been widely used in the market because they are&amp;nbsp;thought to have biological properties and beneficial health effects. Conventional microbiological methods are often time consuming and labor-intensive. Real-time polymerase chain reaction (PCR)&amp;nbsp;method will allow to identify and quantify rapidly Lactobacilli probiotics in food. In this study, the&amp;nbsp;thermal shock protein target region (hsp60) was used to identify Lactobacillus acidophilus species.&amp;nbsp;The limit of detection, specificity, and accuracy were of 104, 100 %, 1
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Kadhim, Ruaa Kamel, and Huda S. A. Al-Hayanni. "Antibacterial and Antibiofilm Activity of Some Lactobacillus spp. Isolated from Natural and Commercial Sources Against XDR Pseudomonas aeruginosa Clinical Isolates." Medical Journal of Babylon 22, no. 1 (2025): 294–300. https://doi.org/10.4103/mjbl.mjbl_1367_23.

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Abstract Background: The emergence of biofilm-forming- and antibiotic-resistant Pseudomonas aeruginosa has renewed efforts to identify safe and natural alternative agents such as probiotics. Objectives: This study aimed to assess both the antibacterial and antibiofilm efficacy of some lactobacilli probiotics isolated from natural and commercial sources against pathogenic P. aeruginosa. Materials and Methods: Clinical P. aeruginosa isolates were isolated and identified from Baghdad hospitals, which were later tested for their ability to resist antibiotics and produce biofilms. Lactobacilli spec
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Fitriani, Victoria Yulita, Budi Suprapti, and Muhammad Amin. "The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics." Journal of Basic and Clinical Physiology and Pharmacology 32, no. 4 (2021): 743–49. http://dx.doi.org/10.1515/jbcpp-2020-0482.

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Abstract Objectives This study aims to determine the characteristics of Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk, respectively as probiotic candidate based on exposure to pH, bile salts, pathogenic bacteria, and antibiotics. Methods In vitro studies were conducted to examine the resistance of Lactobacillus acidophilus and Lactobacillus reuteri in pH 2, 2.5, 3.2, and 7.2, resistance to bile salts, resistance to pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Enterococcus faecalis) and antituberculosis antibiotics. Res
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Leme, Lúcia Aparecida Federighi Pereira, Karina Ferreira Rizzardi, Isis Bolsonaro Santos та Thaís Manzano Parisotto. "Exploring the Relationship between Salivary Levels of TNF-α, Lactobacillus acidophilus, Lactobacillus gasseri, Obesity, and Caries in Early Childhood". Pathogens 11, № 5 (2022): 579. http://dx.doi.org/10.3390/pathogens11050579.

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This research aimed to explore the relationship between tumor necrosis factor-α (TNF-α), Lactobacillus acidophilus (L. acidophilus), Lactobacillus gasseri (L. gasseri), obesity, and early childhood caries. After caries and obesity diagnosis based on the WHO criteria, 94 preschoolers were assessed. Unstimulated saliva was collected for analysis of TNF-α by the Milliplex system and for L. acidophilus and L. gasseri using real-time polymerase chain reaction (RT-PCR). In obese children, each unit increase in the body mass index (BMI), and the TNF-α levels was associated with a one-time increase in
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Taghizadeh, Somayyeh, Tahereh Falsafi, Rouha Kasra Kermanshahi, and Reihaneh Ramezani. "Antagonistic and Immunomodulant Effects of Two Probiotic Strains of Lactobacillus on Clinical Strains of Helicobacter pylori." Galen Medical Journal 9 (October 14, 2020): 1794. http://dx.doi.org/10.31661/gmj.v9i0.1794.

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Background: The present study aimed to evaluate the in vitro and in situ antagonistic effects of Lactobacillus probiotic strains on clinical strains of Helicobacter pylori . Also to investigate their immunomodulation effects on a macrophage cell model. Materials and Methods: Anti-microbial effects of probiotic lactobacilli against H. pylori was assessed using the well and disk diffusion methods. Effects of lactobacilli probiotics strains, as well as their cell-free supernatant on adhesion of H. pylori to MKN-45 gastric epithelial cells, were examined in their presence and absence. Immunomodula
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Клименко, О. Г., та Н. А. Ткаченко. "ВИБІР ПРОБІОТИЧНИХ КУЛЬТУР LACTOBACILLUS ACIDOPHILUS ДЛЯ ВИРОБНИЦТВА ПРОБІОТИЧНИХ СИРІВ". Scientific Works 87, № 2 (2023): 78–88. http://dx.doi.org/10.15673/swonaft.v87i2.2859.

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Стаття присвячена скринінгу пробіотичних культур Lactobacillus acidophilus для виробництва пробіотичних сирів, які користуються попитом на споживчому ринку України. Визначено, що монокультури L. acidophilus La-5 та L. acidophilus LA 02 стійкі до кислого середовища, характерного для рН шлунку, розчину жовчі з масовою часткою 40 %, 0,3 %-вого розчину фенолу та хлористого натрію. Встановлено, що швидкість відмирання клітин L. acidophilus La-5 та L. acidophilus LA 02 при рН=3,0 од. склала 0,23 і 0,32 год-1 відповідно протягом перших 3 год експозиції, а також 0,34 і 0,40 год-1 відповідно – при експ
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Indriyani Chusnadia, Shafira, Atik Kurniawati, Sari Setyaningsih, Erawati Wulandari, and Pudji Astuti. "MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356." Journal of Vocational Health Studies 7, no. 2 (2023): 115–20. http://dx.doi.org/10.20473/jvhs.v7.i2.2023.115-120.

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Background: Dental caries is an oral cavity disease that affects most Indonesians. Lactobacillus acidophilus (L. acidophilus) is one of the bacteria that causes dental caries. Control of bacteria in the form of antibacterial agents is needed to suppress the growth of L. acidophilus. Purple leaves (Graptophyllum pictum L. Griff) are a medicinal plant with antibacterial compounds, namely flavonoids, alkaloids, saponins, and tannins. Purpose: Determine the Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus. Method: The sample consisted of seven groups,
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41

Vieira de Souza, F., R. Roque, J. L. Silva Moreira, et al. "Transfer of antibiotic resistance determinants between lactobacilli isolates from the gastrointestinal tract of chicken." Beneficial Microbes 3, no. 2 (2012): 137–44. http://dx.doi.org/10.3920/bm2011.0058.

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The aim of this study was to assess the potential horizontal transfer of genetic traits for antibiotic resistance between lactobacilli isolated from the chicken gut, both in vitro and in vivo. Thirty-seven Lactobacillus spp. strains isolated from the gizzard, small and large intestines and caeca of free-range broiler chickens showed multi-drug resistance as assessed by disc diffusion assays. The minimum inhibitory concentration (MIC) for vancomycin, tetracycline, erythromycin and chloramphenicol was determined in De Man, Rogosa and Sharpe broth in a microplate assay. Almost all the lactobacill
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42

Widjiastuti, Ira, Adioro Soetojo, and Febriastuti Cahyani. "Anti-glucan effects of propolis ethanol extract on Lactobacillus acidophillus." Dental Journal (Majalah Kedokteran Gigi) 50, no. 1 (2017): 28. http://dx.doi.org/10.20473/j.djmkg.v50.i1.p28-31.

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Background: In deep dentinal caries cases, bacteria mostly found are Lactobacillus acidophilus classified as gram positive bacteria and as facultative aerobes producing glucosyltransferase (GTF) enzyme. GTF enzyme can alter sucrose into glucans. Glucan is sticky and insoluble in water. As a result, GTF enzyme can facilitate plaque formation and microorganism colonization on tooth surface. In addition, Lactobacillus acidophilus also can form acid leading to demineralization of organic and inorganic materials, resulting in dental caries. Multidrug-resistant phenomena, on the other hand, have led
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Sejati, Renny Novita Asri, Rasyidah Rasyidah, and Rizki Amelia Nasution. "Pemanfaatan Limbah Cair Tempe dengan Penambahan Lactobacillus acidophilus Menggunakan Microbial Fuel Cell (MFC) Menjadi Biolistrik." BIO-CONS : Jurnal Biologi dan Konservasi 6, no. 1 (2024): 685–94. http://dx.doi.org/10.31537/biocons.v6i1.1808.

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Teknologi baru yang sedang banyak dikembangkan sebagai penyediaan sumber listrik alternatif salah satunya yaitu Microbial Fuel Cell (MFC). Penelitian ini menggunakan substrat dari limbah cair tempe dan bakteri Lactobacillus acidophilus. Desain sistem MFC yang digunakan yaitu dual-chamber dengan masing-masing reaktor memilki volume 500 ml. Penelitian yang dilakukan pada sistem MFC yaitu limbah cair tempe murni dan limbah cair tempe dengan penambahan bakteri Lactobacillus acidophilus, dengan waktu inkubasi 9 hari. Tujuan penelitian ini adalah untuk mengetahui potensi bakteri Lactobacillus acidop
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Wegmann, Udo, Karin Overweg, Nikki Horn, et al. "Complete Genome Sequence of Lactobacillus johnsonii FI9785, a Competitive Exclusion Agent against Pathogens in Poultry." Journal of Bacteriology 191, no. 22 (2009): 7142–43. http://dx.doi.org/10.1128/jb.01182-09.

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ABSTRACT Lactobacillus johnsonii is a member of the acidophilus group of lactobacilli. Because of their probiotic properties, including attachment to epithelial cells, immunomodulation, and competitive exclusion of pathogens, representatives of this group are being intensively studied. Here we report the complete annotated genome sequence of Lactobacillus johnsonii FI9785, a strain which prevents the colonization of specific-pathogen-free chicks by Clostridium perfringens.
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Lestari, Getha Puji, Desty Ervira Puspaningtyas, Puspita Mardika Sari, Silvia Dewi Styaningrum, and Adi Sucipto. "Skor aktivitas prebiotik tepung growol terhadap Lactobacillus bulgaricus, Streptococcus thermophilus, dan Lactobacillus acidophilus dibanding Escherichia coli." Ilmu Gizi Indonesia 7, no. 2 (2024): 173. http://dx.doi.org/10.35842/ilgi.v7i2.508.

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Latar Belakang: Growol adalah makanan tradisional Kulon Progo, Yogyakarta. Fermentasi singkong menjadi growol terbukti dapat menurunkan kadar gula total, gula reduksi, dan sukrosa, serta meningkatkan kadar serat pangan yang berpotensi sebagai sumber prebiotik. Potensi prebiotik tepung growol sudah dilakukan pada Lactobacillus sp. Potensi prebiotik tepung growol perlu dilakukan pada bakteri probiotik lain. Tujuan: Penelitian ini bertujuan untuk menilai potensi prebiotik tepung growol yang mendukung pertumbuhan dan aktivitas bakteri probiotik, seperti Lactobacillus bulgaricus, Streptococcus ther
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Canchaya, Carlos, Marcus J. Claesson, Gerald F. Fitzgerald, Douwe van Sinderen, and Paul W. O'Toole. "Diversity of the genus Lactobacillus revealed by comparative genomics of five species." Microbiology 152, no. 11 (2006): 3185–96. http://dx.doi.org/10.1099/mic.0.29140-0.

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The genus Lactobacillus contains over 80 recognized species, and is characterized by a high level of diversity, reflected in its complex phylogeny. The authors' recent determination of the genome sequence of Lactobacillus salivarius means that five complete genomes of Lactobacillus species are available for comparative genomics: L. salivarius, L. plantarum, L. acidophilus, L. johnsonii and L. sakei. This paper now shows that there is no extensive synteny of the genome sequences of these five lactobacilli. Phylogeny based on whole-genome alignments suggested that L. salivarius was closer to L.
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Shu, Guo Wei, He Chen, Tao Qin, and Qi Ma. "Effect of the Extract of Fructus tribuli on Growth of Lactobacillus acidophilus." Key Engineering Materials 480-481 (June 2011): 261–65. http://dx.doi.org/10.4028/www.scientific.net/kem.480-481.261.

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Effect of the extract of Fructus tribuli (EFT) on growth of Lactobacillus acidophilus LA04, LA05, LA06 and LA09 was studied by measuring optical density at 600nm (OD600) and pH using MRS culture media as the control. The addition of EFT (v/v) was 1%, 2%, 3%, 4% and 5%. Results were as follows: addition of EFT could promote the growth of Lactobacillus acidophilus LA09, The optimum concentration of EFT in MRS media was 1% at incubation 24h, but addition of EFT on growth of Lactobacillus acidophilus LA04, LA05 and LA06 has no significant effect.
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Hosoi, Tomohiro, Akio Ametani, Kan Kiuchi, and Shuichi Kaminogawa. "Improved growth and viability of lactobacilli in the presence of Bacillus subtilis (natto), catalase, or subtilisin." Canadian Journal of Microbiology 46, no. 10 (2000): 892–97. http://dx.doi.org/10.1139/w00-070.

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In an effort to demonstrate the potential usefulness of Bacillus subtilis (natto) as a probiotic, we examined the effect of this organism on the growth of three strains of lactobacilli co-cultured aerobically in vitro. Addition of B. subtilis (natto) to the culture medium resulted in an increase in the number of viable cells of all lactobacilli tested. Since B. subtilis (natto) can produce catalase, which has been reported to exhibit a similar growth-promoting effect on lactobacilli, we also examined the effect of bovine catalase on the growth of Lactobacillus reuteri JCM 1112 and L. acidophil
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Wahyuningsih, Ani, Nyoman Suthama, Vitus Dwi Yunianto, and Lilik Krismiyanto. "Penambahan Kombinasi Ekstrak Buah Mengkudu (Morinda citrifolia l.) dan Lactobacillus acidophilus dalam Ransum Terhadap Kinerja Hati Ayam Broiler." JURNAL GALUNG TROPIKA 9, no. 3 (2021): 314–23. http://dx.doi.org/10.31850/jgt.v9i3.682.

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Noni fruit extract as a phytobiotic and Lactobacillus acidophilus as a probiotic is used to maintain body health and liver function in broiler chickens. The aim of this study was to determine the effect of adding a combination of noni fruit extract and Lactobacillus acidophilus in the liver ration based on serum glutamate oxaloacetate concentration transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and relative liver weight in broiler chickens. Research materials included 189 broiler chickens (body weight 40.98 ± 1.27 g), noni fruit extract and Lactobacillus acidophilus as treat
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Goel, Shubhi, Chandra Shekar B R, and Chandan S. "Evaluation of Antioxidant and Antimicrobial Efficacy of Camellia Sinensis and Alstonia Scholaris Extracts on Streptococcus Mutans and Lactobacillus Acidophilus - An in Vitro Study." Biomedical and Pharmacology Journal 14, no. 1 (2021): 455–65. http://dx.doi.org/10.13005/bpj/2145.

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Context Dental caries is showing an upward trend in India and there is a need to explore innovative strategies to prevent the disease. Literature evaluating antibacterial activity of Camellia sinensis and Alstonia scholaris plant extracts on Streptococcus mutans and Lactobacillus acidophilus is practically non-existent. Objectives - To assess the minimum inhibitory concentration (MIC) and antimicrobial efficacy of Camellia sinensis and Alstonia scholaris on S. mutans and L. acidophilus. Materials and method: This was an in vitro study carried over a period of three months. The leaves of Camell
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