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Artykuły w czasopismach na temat „Luminol-dependent Chemiluminescence”

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Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Shani, W. Krol, J., Z. Czuba, and S. Scheller. "Modulating Luminol-Dependent Chemiluminescence Of Neutrophils By Flavones." Zeitschrift für Naturforschung C 47, no. 11-12 (1992): 889–92. http://dx.doi.org/10.1515/znc-1992-11-1216.

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The effect of 14 flavones on luminol-dependent chemiluminescence of neutrophils was studied in vitro. Chemiluminescence was used in this study as an indicator for the production of a reactive oxygen species by neutrophils, stimulated by phorbol myristate acetate. While flavone- 8-acetic acid, and most of the compounds tested, inhibited chemiluminescence, flavone and its 5-hydroxy-7-methoxy derivatives enhanced it by up to 150%. The most active inhibitors of photon emission were the glycosides. These results indicate that lipophilicity and some structural determinants modulate the chemiluminesc

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2

Kurtasova, I. M., T. V. Lubnina та E. V. Safontseva. "Сhanges in functional activity of peripheral blood neutrophils in young children with recurrent respiratory infection". Medical Immunology (Russia) 24, № 2 (2022): 407–12. http://dx.doi.org/10.15789/1563-0625-cif-2407.

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Neutrophils characterized by high mobility and ability for quick and accurate reresponse upon homeostatic changes. These changes primarily occur at the inflammation site. The pathogen elimination depends on the phagocytic activity of neutrophils. The data from past decades have revisited the role of neutrophils and their involvement in changing the human cellular and humoral immunity. Neutrophils are not only effector cells, but also regulatory cells of both innate and adaptive immunity. Our purpose was to study phagocytic activity and parameters of oxygen-dependent metabolism of peripheral bl

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Dulin, Anne M., Max J. Paape, and Stephen C. Nickerson. "Comparison of phagocytosis and chemiluminescence by blood and mammary gland neutrophils from multiparous and nulliparous cows." American Journal of Veterinary Research 49, no. 2 (1988): 172–77. https://doi.org/10.2460/ajvr.1988.49.02.172.

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SUMMARY Neutrophils were isolated from the blood and mammaiy gland of 3 multiparous lactating cows and 3 nulliparous heifers. Neutrophil function was evaluated by phagocytosis and luminol-dependent chemiluminescence. Peroxidase activity was detected by use of transmission electron microscopy. Compared with that for blood neutrophils, percentage of phagocytosis was 9.6% lower for neutrophils isolated from the mammary gland of lactating cows, but this difference was not observed between neutrophils isolated from the mammary gland and from the blood of heifers. Similarly, after subtraction of che

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4

Davies, B., and S. W. Edwards. "Inhibition of myeloperoxidase by salicylhydroxamic acid." Biochemical Journal 258, no. 3 (1989): 801–6. http://dx.doi.org/10.1042/bj2580801.

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Salicylhydroxamic acid inhibited the luminol-dependent chemiluminescence of human neutrophils stimulated by phorbol 12-myristate 13-acetate or the chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (fMet-Leu-Phe). This compound had no inhibitory effect on the kinetics of O2.- generation or O2 uptake during the respiratory burst, but inhibited both the peroxidative activity of purified myeloperoxidase and the chemiluminescence generated by a cell-free myeloperoxidase/H2O2 system. The concentration of salicylhydroxamic acid necessary for complete inhibition of myeloperoxidase activity wa

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5

Wang, J. F., P. Komarov, H. Sies, and H. de Groot. "Contribution of nitric oxide synthase to luminol-dependent chemiluminescence generated by phorbol-ester-activated Kupffer cells." Biochemical Journal 279, no. 1 (1991): 311–14. http://dx.doi.org/10.1042/bj2790311.

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Phorbol 12-myristate 13-acetate-induced luminol chemiluminescence in rat Kupffer cells was doubled by the addition of L-arginine and significantly (up to 70%) inhibited by NG-nitro-L-arginine and NG-monomethyl-L-arginine, competitive inhibitors of L-arginine-dependent nitric oxide (NO) formation. The release of superoxide anion (O2-) by NADPH oxidase was neither affected by L-arginine nor by the inhibitors. Only very slight luminol chemiluminescence was detectable in lipopolysaccharide-pretreated Kupffer cells, a condition in which significant amounts of NO were formed but no O2-. In a cell-fr

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6

Slepov, E. V., R. A. Zukov, M. S. Serbaeva, et al. "Possibility of modification of peripheral blood neutrophils functional activity during chemoradiotherapy in patients with anorectal cancer." Cancer Urology 19, no. 1 (2023): 133–40. http://dx.doi.org/10.17650/1726-9776-2023-19-1-133-140.

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Background. Ionizing radiation is an effective antitumor therapy, but it has a serious negative effect on the immune system requiring the use of radiation reaction prevention and reduction methods. Neutrophils are a sensitive element of the immune system both in interaction with tumor tissue and in response to radiation injury.Aim. To evaluate functional activity of peripheral blood neutrophils by chemiluminescent analysis in patients with anorectal cancer after radiotherapy.Materials and methods. The study included 80 patients with anorectal cancer. Patients received chemo- and radio-therapy

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7

Thomas, Virginia L., Barbara A. Sanford, Marcia S. Driscoll, Daniel T. Casto, and Rajam S. Ramamurthy. "Luminol-dependent chemiluminescence microassay for phagocytic function." Journal of Immunological Methods 111, no. 2 (1988): 227–32. http://dx.doi.org/10.1016/0022-1759(88)90131-7.

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8

Chang, Yung-Fu, and Harland W. Renshaw. "Pasteurella haemolytica leukotoxin: Comparison of 51chromium-release, trypan blue dye exclusion, and luminol-dependent chemiluminescence-inhibition assays for sensitivity in detecting leukotoxin activity." American Journal of Veterinary Research 47, no. 1 (1986): 134–38. https://doi.org/10.2460/ajvr.1986.47.01.134.

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SUMMARY Dilutions of concentrated, dialyzed Pasteurella haemolytica culture supernatant were caused to react with bovine neutrophil (pmn) suspensions, and then the trypan blue dye exclusion (tbde), 51chromium (51Cr)-release, and luminol-dependent chemiluminescence-inhibition (ldcll) assays were done to compare their relative sensitivities in detecting biological activity of Ρ haemolytica leukotoxin (cytotoxin). The culture supernatant was concentrated approximately 200:1, and when caused to react as an undiluted preparation with bovine pmn, it was cytotoxic for 38.6% and 80.4% of pmn as determ

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9

Рязанцева, Лариса Тихоновна, and Валерий Павлович Октябрьский. "DETERMINATION OF ANTIOXIDANT POTENTIAL OF SUBSTANCES OF DIFFERENT NATURE IN THE SYSTEM GENERATING SUPEROXIDE ANION RADICAL USING THE METHOD OF MATHEMATICAL MODELING." Вестник Тверского государственного университета. Серия: Химия, no. 3(41) (November 10, 2020): 79–87. http://dx.doi.org/10.26456/vtchem2020.3.9.

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С использованием метода математического моделирования кинетики люминолзависимой хемилюминесценции изучено действие некоторых антиоксидантов (АО) на реакцию окисления люминола супероксидными анион-радикалами, образующимися в системе ТЕМЭД-рибофлавин. Установлено, что для описания вклада антиоксидантов в изменение кинетики люминолзависимой хемилюминесценции (ХЛ) достаточно использовать единственную реакцию взаимодействия антиоксиданта со свободным радикалом. Предложено использование константы скорости этой реакции в качестве меры «антиоксидантной активности». Using the method of mathematical mod

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10

Žekonis, Gediminas, Jonas Žekonis, Renata Šadzevičienė, Genovaitė Šimonienė, and Egidijus Kėvelaitis. "Effect of Perilla frutescens aqueous extract on free radical production by human neutrophil leukocytes." Medicina 44, no. 9 (2008): 699. http://dx.doi.org/10.3390/medicina44090090.

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Objective. The present study was intended to evaluate the antioxidant properties of aqueous extract of the Perilla frutescens (L.) Britton. Material and methods. The antioxidant properties of Perilla frutescens were analyzed employing neutrophil leukocytes stimulated by the nonopsonized Escherichia coli. The neutrophil leukocytes were affected by adding an aqueous extract of Perilla. The generation of the reactive oxygen species by neutrophil leukocytes was investigated using assessment of luminol- and lucigenin-dependent chemiluminescence. Results. We found out that the treatment of neutrophi

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11

Pieńkos, Milena, and Beata Zadykowicz. "Computational Insights on the Mechanism of the Chemiluminescence Reaction of New Group of Chemiluminogens—10-Methyl-9-thiophenoxycarbonylacridinium Cations." International Journal of Molecular Sciences 21, no. 12 (2020): 4417. http://dx.doi.org/10.3390/ijms21124417.

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Immunodiagnostics, in which one of the promising procedures is the chemiluminescent labelling, is essential to facilitate the detection of infections in a human organism. One of the standards commonly used in luminometric assays is luminol, which characterized by low quantum yield in aqueous environments. Acridinium esters have better characteristics in this topic. Therefore, the search for new derivatives, especially those characterized by the higher quantum yield of chemiluminescence, is one of the aims of the research undertaken. Using the proposed mechanism of chemiluminescence, we examine

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12

Domzig, W., and F. E. Maly. "Dissociation of Natural Killing and Luminol-dependent Chemiluminescence." Immunobiology 169, no. 2 (1985): 162–74. http://dx.doi.org/10.1016/s0171-2985(85)80030-9.

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SHULT, P., F. GRAZIANO, and W. BUSSE. "Enhanced eosinophil luminol-dependent chemiluminescence in allergic rhinitis." Journal of Allergy and Clinical Immunology 77, no. 5 (1986): 702–8. http://dx.doi.org/10.1016/0091-6749(86)90414-8.

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CLUZEL, M., M. DAMON, P. CHANEZ, et al. "Enhanced alveolar cell luminol-dependent chemiluminescence in asthma." Journal of Allergy and Clinical Immunology 80, no. 2 (1987): 195–201. http://dx.doi.org/10.1016/0091-6749(87)90129-1.

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15

Bednar, Martin M., Richard H. Dooley, Ricardo Tapanes, Jennifer C. Lublin, and Cordell E. Gross. "Ticlopidine augments luminol-dependent chemiluminescence in human neutrophils." Journal of Bioluminescence and Chemiluminescence 10, no. 2 (1995): 85–89. http://dx.doi.org/10.1002/bio.1170100204.

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Al Hashimi, A. H. M., F. H. Mohammad, and E. A. Abbass Al Krawi. "Inhibition of luminol-dependent chemiluminescence of human granulocytes by low doses of inorganic mercury." Eastern Mediterranean Health Journal 6, no. 2-3 (2000): 425–31. http://dx.doi.org/10.26719/2000.6.2-3.425.

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HgCl2, added in vitro to human granulocytes in whole blood, caused a marked inhibitory effect on the luminol-dependent chemiluminescence induced by BaS04 crystals in suspension of these cells. The effect was both dose- and time-dependent when BaS04 was used to stimulate the oxidative burst in granulocytes. Incubation with the highest concentration of HgCl2 used [10 mmol/L], however, did not cause disruption of the membranes of granulocytes. The effect of HgCl2 on the granulocytes was irreversible following washing of the HgCl2-treated cells with phosphate buffered saline. HgCl2 did not affect

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17

Zasimauskas, Darius, and Gediminas Žekonis. "Effect of smoking on neutrophil oxidative metabolism." Medicina 44, no. 3 (2007): 195. http://dx.doi.org/10.3390/medicina44030025.

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Alterations in neutrophil function by tobacco products may play a central role in the pathogenesis of periodontal diseases and several smoking-related systemic diseases. The aim of the study was to evaluate the effect of smoking on neutrophil oxidative metabolism. Materials and methods. The study included 17 smoking men free of systemic diseases who were referred for treatment of various odontological diseases to outpatient department of Kaunas University of Medicine Hospital. The age of subjects varied from 22 to 43 years. All subjects answered the questions about smoking habits. Clinical exa

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18

Zakiev, T. Z., S. R. Tuysin, O. V. Galimov, A. R. Gil’fanov, and R. D. Sagdeev. "Studying the processes of free-radical oxidation in patients with purulent-inflammatory diseases of soft tissues." Kazan medical journal 96, no. 3 (2015): 302–6. http://dx.doi.org/10.17750/kmj2015-302.

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Aim. To study the effects of combined dressings on the processes of free radical oxidation in patients with purulent wounds.Methods. The performance of luminol-dependent chemiluminescence of whole blood from 30 healthy donors and 122 patients with purulent wounds, as well as tissue chemiluminescence of festering wounds tissue homogenates were examined before and after the treatment. To assess the free radical oxidation in vitro, spontaneous and zymosan-induced whole blood chemiluminescence measurement, iron-induced chemiluminescence assessment of festering wounds tissue homogenates were perfor

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19

Tschakert, Jochen, and Wolfgang Voelter. "Festphasensynthese von Muramyldipeptidderivaten und Untersuchung ihrer biologischen Aktivität / Solid Phase Synthesis of Muramyl Dipeptide Derivatives and Investigations on their Biological Activities." Zeitschrift für Naturforschung B 49, no. 5 (1994): 702–16. http://dx.doi.org/10.1515/znb-1994-0524.

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New muramyl dipeptide derivatives with exchanged carbohydrate residues are described. Each derivative is synthesized via a solid phase synthesis using an aminomethyl anchor resin. All synthetic products can be isolated in good yields. Their biological activities are tested by the luminol-dependent chemiluminescence associated with the phagocytosis of opsonized zymosan by granulocytes.

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20

Terai, Akiko, Masae Amamiya, Yasuyoshi Moritsu, Shun Ichikawa, and Hajime Nagahata. "Analysis of luminol-dependent chemiluminescence response in laying hen blood." Japanese poultry science 38, no. 5 (2001): J23—J27. http://dx.doi.org/10.2141/jpsa.38.j23.

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Yue, Ling, and Yi-Tong Liu. "Mechanistic Insight into pH-Dependent Luminol Chemiluminescence in Aqueous Solution." Journal of Physical Chemistry B 124, no. 35 (2020): 7682–93. http://dx.doi.org/10.1021/acs.jpcb.0c06301.

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Mushtakova, V. M., and V. V. Rogovin. "Clofibrate and dalargin increase luminol-dependent chemiluminescence of mouse blood." Bulletin of Experimental Biology and Medicine 144, no. 3 (2007): 317–18. http://dx.doi.org/10.1007/s10517-007-0321-6.

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FRENCH, RAYMOND C., KARL E. HERBERT, DAVID L. SCOTT, and DAVID PERRETT. "Effect of penicillamine on luminol-dependent chemiluminescence in human leucocytes." Biochemical Society Transactions 16, no. 5 (1988): 891. http://dx.doi.org/10.1042/bst0160891.

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Cogliati, C., D. Vaggi, M. Fare, C. Sielewicz, and L. Brunelli. "Luminol-dependent chemiluminescence of human neonatal polymorphonuclear leukocyte (PMN) activity." European Journal of Pediatrics 145, no. 1-2 (1986): 159–60. http://dx.doi.org/10.1007/bf00441887.

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Wojtecka-Lukasik, E., A. Grzybowska-Kowalczyk, D. Maslinska, D. Szukiewicz, W. Schunack, and S. Maslinski. "Effect of histamine chloramine on luminol-dependent chemiluminescence of granulocytes." Inflammation Research 57, S1 (2008): 19–20. http://dx.doi.org/10.1007/s00011-007-0609-z.

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Dahlgren, Claes, Per Follin, Agneta Johansson, Ronny Lock, and Kristina Orselius. "Localization of the luminol-dependent chemiluminescence reaction in human granulocytes." Journal of Bioluminescence and Chemiluminescence 4, no. 1 (1989): 263–66. http://dx.doi.org/10.1002/bio.1170040137.

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Klimshina, O. A., and A. N. Erin. "Luminol- and lucigenin-dependent chemiluminescence during autooxidation of 6-hydroxydopamine." Bulletin of Experimental Biology and Medicine 111, no. 6 (1991): 794–97. http://dx.doi.org/10.1007/bf00840185.

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Castro, Laura, Marı́a Noel Alvarez, and Rafael Radi. "Modulatory Role of Nitric Oxide on Superoxide-Dependent Luminol Chemiluminescence." Archives of Biochemistry and Biophysics 333, no. 1 (1996): 179–88. http://dx.doi.org/10.1006/abbi.1996.0379.

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Dunaevskaya, S. S., M. V. Cherednichenko, and G. V. Makarskaya. "Features in chemiluminescence of neutrophils in cardiac surgical patients in the postoperative period." Siberian Medical Review, no. 2 (2022): 56–61. http://dx.doi.org/10.20333/25000136-2022-2-56-61.

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The aim of the research. To study the changes in luminol-dependent chemiluminescence of neutrophils in cardiac surgical patients in the early postoperative period. Material and methods. A cohort prospective study was conducted, enrolling 45 cardiac surgical patients aft er medial sternotomy. Th e gender composition of the clinical group was 37 (82.2 %) men and 8 (17.8 %) women, the average age in the group was 59.29 [37.2; 72.1] years. Chemiluminescent analyses were performed in the preoperative period and 1-3 days after surgery. Results. Evaluation of functional activity of leukocytes is an i

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Ikkes, L. A., A. A. Savchenko, G. P. Martinova, and I. I. Gvozdev. "Granulocyte-macrophage colony-stimulating factor: prospects for use in children with infectious mononucleosis." Voprosy praktičeskoj pediatrii 17, no. 5 (2022): 43–51. http://dx.doi.org/10.20953/1817-7646-2022-5-43-51.

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Objective. To analyze the production of active oxygen species (AOS) by peripheral blood neutrophils in children with infectious mononucleosis (IM) after their exposure to granulocyte-macrophage colony-stimulating factor (GM-CSF) in vitro. Patients and methods. We examined 78 children aged 3 to 11 years in the acute period of IM (including 47 children aged 3–6 years and 31 children aged 7–11 years). The control group comprised 40 healthy children matched for age. Luminoldependent chemiluminescence (CL) of peripheral blood neutrophils was assessed using the method of De Sole et al. The following

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31

Dudina, M. A., A. A. Savchenko, S. A. Dogadin, and I. I. Gvozdev. "The chemiluminescent and enzymatic activity of blood neutrophils in patients with Graves' disease depending on hyperthyroidism compensation." Clinical and experimental thyroidology 18, no. 1 (2022): 4–14. http://dx.doi.org/10.14341/ket12717.

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BACKGROUND: Oxidative stress in Graves’ disease can potentiate the cytotoxicity of neutrophils and support autoimmune inflammation. Elimination of hyperthyroidism with conservative therapy with thiamazole only in some cases leads to remission, which determines the necessity to search an immunological markers for etiotropic therapeutic approaches in treatment of the disease.AIM: To study the chemiluminescent and enzymatic activity of peripheral blood neutrophils in patients with Graves’ disease depending on hyperthyroidism compensation to determine the intracellular targets of immunotropic trea

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Conroy, PT, MJ Elliott, PN Platt, and M. Holden. "Neutrophil function and cardiopulmonary bypass in humans. The effects of glucose and non-glucose containing bypass pump priming fluids." Perfusion 1, no. 2 (1986): 103–16. http://dx.doi.org/10.1177/026765918600100205.

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Defective polymorphonuclear neutrophil function during cardiopulmonary bypass (CPB) has been implicated as a cause of postoperative infection following open-heart surgery. Neutrophil function is known to be impaired in poorly controlled diabetics with elevations of blood glucose concentrations of the order which occur frequently during CPB when glucose containing priming fluids are used. Neutrophil function, as measured by bactericidal assay, and neutrophil and whole blood luminol dependent chemiluminescence, was studied in two groups of 1 2 patients undergoing coronary artery bypass graft sur

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Novikov, V., E. Yablokova, I. Shaev, N. Novikova, and E. Fesenko. "COMPARATIVE ANALYSIS OF THE INHIBITORY EFFECTS OF THE HYPOMAGNETIC FIELD AND COMBINED MAGNETIC FIELDS ON THE PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS) BY NEUTROFILS." Russian Journal of Biological Physics and Chemisrty 8, no. 2 (2024): 143–50. http://dx.doi.org/10.29039/rusjbpc.2023.0601.

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The article reveals that a decrease in the background production of reactive oxygen species (ROS) in the peritoneal neutrophils of mice after a short-term (40 minutes) stay in hypomagnetic conditions (residual field  10 nT) at physiological temperatures, detected by the method of lucigenin-dependent chemiluminescence, is not accompanied by a violation of chemiluminescent response to respiratory burst activators: formylated peptide N-formyl-Met-Leu-Phe (fMLF) and phorbol ester of phorbol-12-meristat-13-acetate (PMA). These results were obtained by activated chemiluminescence using lucigenin or

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Archer, S. L., D. P. Nelson, and E. K. Weir. "Detection of activated O2 species in vitro and in rat lungs by chemiluminescence." Journal of Applied Physiology 67, no. 5 (1989): 1912–21. http://dx.doi.org/10.1152/jappl.1989.67.5.1912.

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This study used chemiluminescence, an “on-line” photon-counting technique, to detect and characterize activated O2 species in vitro and in isolated rat lungs. The sensitivity and specificity of enhanced chemiluminescence for superoxide anion (O2-.) and hydrogen peroxide (H2O2) was evaluated in vitro. The effect of media conditions (such as O2 tension, albumin concentration, and sulfhydryl group availability) on luminescence was assessed in vitro. Xanthine-xanthine oxidase (X-XO) primarily produced superoxide anion in vitro. Enhanced chemiluminescence varied directly with the dose of luminescen

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Darenskaya, Marina, Boris Gubanov, Olga Smirnova, Sergey I. Kolesnikov, and Lyubov Kolesnikova. "Patients with Mechanical Jaundice: Neutrophils’ functional activity (Induced luminol-dependent chemiluminescence)." Free Radical Biology and Medicine 180 (February 2022): s59. http://dx.doi.org/10.1016/j.freeradbiomed.2021.12.135.

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Diesing, L., S. Steuber, and J. S. Ahmed. "Variant Specific Opsonization of Trypanosoma evansi Measured by Luminol-dependent Chemiluminescence." Immunobiology 169, no. 2 (1985): 139–46. http://dx.doi.org/10.1016/s0171-2985(85)80028-0.

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Vilim, Vladimir, and Jiri Wilhelm. "What do we measure by a luminol-dependent chemiluminescence of phagocytes?" Free Radical Biology and Medicine 6, no. 6 (1989): 623–29. http://dx.doi.org/10.1016/0891-5849(89)90070-1.

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Reibnegger, Gilbert, Dietmar Fuchs, Christian Murr, Manfred P. Dierich, Wolfgang Pfleiderer, and Helmut Wachter. "Effects of pteridines on luminol-dependent chemiluminescence induced by chloramine-T." Free Radical Biology and Medicine 18, no. 3 (1995): 515–23. http://dx.doi.org/10.1016/0891-5849(94)00164-f.

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Catz, Sergio D., Maria C. Carreras, and Juan J. Poderoso. "Nitric oxide synthase inhibitors decrease human polymorphonuclear leukocyte luminol-dependent chemiluminescence." Free Radical Biology and Medicine 19, no. 6 (1995): 741–48. http://dx.doi.org/10.1016/0891-5849(95)00065-6.

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Li, Na, Wei Wang, Dayong Tian, and Hua Cui. "pH-dependent catalytic properties of Pd–Ag nanoparticles in luminol chemiluminescence." Chemical Communications 46, no. 9 (2010): 1520. http://dx.doi.org/10.1039/b920736h.

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KUMAZAWA, Norichika H., Naoki MORIMOTO, and Yoshiharu OKAMOTO. "Luminol-Dependent Chemiluminescence of Hemocytes Derived from Marine and Estuarine Molluscs." Journal of Veterinary Medical Science 55, no. 2 (1993): 287–90. http://dx.doi.org/10.1292/jvms.55.287.

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Zappacosta, B., A. Mordente, S. Persichilli, B. Giardina, and P. De Sole. "Effect of homocysteine on polymorphonuclear leukocyte activity and luminol-dependent chemiluminescence." Luminescence 15, no. 4 (2000): 257–60. http://dx.doi.org/10.1002/1522-7243(200007/08)15:4<257::aid-bio594>3.0.co;2-7.

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Haqqani, Arsalan S., Jagdeep K. Sandhu, and H. Chaim Birnboim. "A Myeloperoxidase-Specific Assay Based upon Bromide-Dependent Chemiluminescence of Luminol." Analytical Biochemistry 273, no. 1 (1999): 126–32. http://dx.doi.org/10.1006/abio.1999.4206.

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O'Keefe, D. A., D. R. James, W. R. Ware, and N. O. Petersen. "Kinetics of a rapid, luminol dependent chemiluminescence signal induced in HL-60 cells by amphotericin B and other stimulants." Biochemistry and Cell Biology 69, no. 9 (1991): 618–23. http://dx.doi.org/10.1139/o91-091.

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Addition of the polyene antibiotic amphotericin B or tissue culture medium to nondifferentiated HL-60 cells in the presence of luminol induces a chemiluminescence signal that reaches a peak value within a few seconds and decays exponentially in less than a minute. The kinetics of the signal and its modulation by superoxide dismutase, catalase, and horseradish peroxidase are consistent with a series of solution biochemical processes with a rate-determining step corresponding to the disproportionation of a luminal–superoxide complex. The effects of the enzymes demonstrate that superoxide is a pr

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Khan, Haseeb Ahmad. "Zymosan-induced luminol-dependent chemiluminescence response of circulating and extravasated leukocytes in experimental sepsis." Mediators of Inflammation 13, no. 2 (2004): 123–25. http://dx.doi.org/10.1080/09629350410001688558.

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This study examines a concurrent profiling of circulating and extravasated polymorphonuclear leukocytes (PMNs) in a rat model of experimental sepsis. Fecal peritonitis was induced in Wistar male rats by intraperitoneal instillation of a fecal suspension in saline (1:1 w/v). Blood and peritoneal fluid were collected 8 h following fecal inoculation for the evaluation of inflammatory response of PMNs using zymosan-induced luminol-dependent chemiluminescence. Fifty microliters of pre-diluted blood or peritoneal fluid samples were mixed with 150 μl of reaction mixture (4×10−4M luminol+50 μg opsoniz

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Rogovine, V. V., V. M. Mushtakova, and V. A. Fomina. "Estimation of Xenobiotic Effects on the Peroxidase-Dependent Plant Immunity as a Means of Ecological Control." Water Quality Research Journal 32, no. 4 (1997): 829–38. http://dx.doi.org/10.2166/wqrj.1997.045.

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Abstract The effect of various dyes, ions of heavy metals (Cd2+, Pb2+, Sn2+, Hg2+, Cu2+) and gaseous waste from thermal power stations (H2S, SO2, NO, NH3) on the peroxi-dase-dependent immunity of pea roots (Pisum sativum) has been studied by luminol-enhanced chemiluminescence. It has been found that copper toxicity could be comparable to that of mercury. Fabric dyes dis-azodye “direct” red and “vat” violet at concentrations of 10-5 M reduced plant chemiluminescence as much as 50%. Hydrogen sulfide turned out to be the most toxic among the gases of the industrial gaseous waste. Considering the

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Dogadin, Sergey A., Margarita A. Dudina, Andrey A. Savchenko, Vladimir A. Mankovskiy, and Ivan I. Gvozdev. "The respiratory explosion activity of neutrophils in blood from patients with Graves' disease manifestation." Problems of Endocrinology 63, no. 1 (2017): 4–8. http://dx.doi.org/10.14341/probl20176314-8.

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 Objective. To study respiratory burst activity in neutrophilic granulocytes in the onset of Graves’ disease (GD). Material and methods. Twenty-six females aged 18—55 years (mean age, 40.7±13.2 years), with the diagnosis of Graves’ disease verified for the first time, were enrolled in this study. Spontaneous and zymosan-induced chemiluminescence (CL) was assessed using a CL3606 36-channel analyzer for 90 min. The following parameters of the chemiluminescence curve were determined: time to attain the maximum (Tmax), the maximum intensity (Imax), and the area under the curve (S).&#x0

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Lieberman, Michael M., and Robert C. Allen. "Opsonic activity of antisera to ribosomal vaccine fractions with live and formalinized Pseudomonas aeruginosa." Canadian Journal of Microbiology 32, no. 6 (1986): 531–33. http://dx.doi.org/10.1139/m86-098.

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The opsonic capacity of antisera to Pseudomonas aeruginosa ribosomal vaccine fractions was determined by a chemiluminescent technique. Antiserum to a vaccine fraction ("peak A") containing lipopolysaccharide (antiserum A), and antiserum to a vaccine fraction ("peak B"), which did not contain detectable amounts of lipopolysaccharide (antiserum B), were used to opsonify live or formalin-treated bacteria. Polymorphonuclear leukocytes were then stimulated by the opsonified bacteria in the presence of the chemiluminigenic probe, luminol, resulting in the observed chemiluminescence. The data obtaine

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Chang, Yung-Fu, Harland W. Renshaw, and Ry Young. "Pneumonie pasteurellosis: Examination of typable and untypable Pasteurella haemolytica strains for leukotoxin production, plasmid content, and antimicrobial susceptibility." American Journal of Veterinary Research 48, no. 3 (1987): 378–84. https://doi.org/10.2460/ajvr.1987.48.03.378.

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SUMMARY Plasmid DNA screening experiments were conducted to determine whether a relationship existed between the presence of plasmids and antibiotic resistance in Pasteurella haemolytica or the capability to produce hemolysin or leukotoxin (cytotoxin). Regardless of plasmid content, all P haemolytica isolates produced characteristic hemolysis on blood agar plates. Similarly, standardized suspensions of living bacteria and sterile concentrated (approx 200:1) culture supernatant from strains representing each of the 15 recognized P haemolytica serotypes and 7 field strains of P haemolytica (biot

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QIAN, Mingwei, John W. EATON, and Simon P. WOLFF. "Cyanate-mediated inhibition of neutrophil myeloperoxidase activity." Biochemical Journal 326, no. 1 (1997): 159–66. http://dx.doi.org/10.1042/bj3260159.

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Cyanate (CNO-) forms spontaneously in solutions containing urea, and is present in urine and the body fluids of uraemic patients. We have explored the possibility that CNO- might be one of the unknown substances responsible for the reported impairment, by urine and uraemic plasma, of neutrophil oxidative metabolism (especially as measured by luminol-enhanced chemiluminescence). Luminol-enhanced chemiluminescence generated by human neutrophils derives predominantly from the activity of myeloperoxidase (MPO) which produces hypochlorous acid from H2O2 and Cl-. We hypothesized that CNO- (which res

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