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Artykuły w czasopismach na temat „Male clones”

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Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Arat, S., S. Pabuccuoglu, H. Sagirkaya, et al. "22 SEMEN AND REPRODUCTIVE PROFILES OF CLONED ANATOLIAN GREY CATTLE." Reproduction, Fertility and Development 27, no. 1 (2015): 103. http://dx.doi.org/10.1071/rdv27n1ab22.

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Anatolian grey cattle (endangered native Anatolian cattle) as 1 male (clone 1) and 4 females (clones 2–5) were produced from cells of 1 male and 1 female cattle by somatic cell nuclear transfer (SCNT) in a previous study. In this study, we examined the reproductive potential of these cloned animals, which are now 4 and 5 years old. The parameters evaluated by phase contrast microscopy for motility, TUNEL for DNA fragmentation, eosin staining for viability, Hoechst 33258 staining and hypo-osmotic swelling test (HOST) for membrane integrity, and fluorescein isothiocyanate-Pisum sativum agglutini

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2

Choi, E. G., X. J. Yin, H. S. Lee, et al. "30 GROWTH AND REPRODUCTIVE SUCCESS OF CLONED MALE CATS DERIVED FROM ADULT SOMATIC CELL NUCLEAR TRANSFER." Reproduction, Fertility and Development 19, no. 1 (2007): 133. http://dx.doi.org/10.1071/rdv19n1ab30.

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There have been very few published reports on the normal growth and fertility of cloned cats derived from adult somatic cell nuclear transfer (SCNT). Here, we report studies investigating the growth and fertility of cloned male cats produced by SCNT. This study was designed to investigate the reproductive success by natural breeding of cloned male cats with domestic female cats and to measure endocrine hormone profiles related to male reproduction such as testosterone, LH, and FSH. Clones A, B, and C produced 3, 2, and 4 kittens, respectively, after natural mating with 4 domestic female cats,

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3

Zimmer, Régis, Alon Haberfeld, and Ann M. Verrinder Gibbins. "Microisolation of the chicken Z chromosome and construction off microclone libraries." Genome 40, no. 6 (1997): 865–72. http://dx.doi.org/10.1139/g97-812.

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A simple method was used to adapt a standard light microscope for the collection of chicken Z chromosomes from mitotic-metaphase spreads. The DNA of the collected chromosomes was enzymatically amplified using a partially degenerate primer. The resulting sequences, within a size range of 200–800 bp, were cloned to produce a Z chromosome DNA library, using blunt-end ligation into a SmaI-digested pUC18 plasmid (the SureClone system; Pharmacia, U.S.A.). The microcloning experiments produced 1250 clones; the size range of the cloned inserts was 250–800 bp, with an average of 480 bp (176 clones exam

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4

Heyman, Y., C. Richard, L. Delatouche, J. P. Renard, and P. Chavatte-Palmer. "32 CHARACTERISTICS OF BOVINE CLONE OFFSPRING (F1): COMPARISON WITH CLONES." Reproduction, Fertility and Development 21, no. 1 (2009): 116. http://dx.doi.org/10.1071/rdv21n1ab32.

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Information on clone offspring (F1) remains limited, especially in species with a long intergeneration interval such as cattle. In the present study, we report data on calves born from cloned females at the INRA experimental unit. Characteristics of these offspring are compared to that of clones or AI control animals born and raised in the same farm. Currently, 39 F1 have been obtained after AI of 64 cloned heifers from 3 different Holstein genotypes, with semen from 6 different Holstein sires. All calves were naturally delivered at term with a mean easy calving score of 1.58 and a normal preg

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5

Schoen, D. J., and S. C. Stewart. "Variation in Male Fertilities and Pairwise Mating Probabilities in Picea glauca." Genetics 116, no. 1 (1987): 141–52. http://dx.doi.org/10.1093/genetics/116.1.141.

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ABSTRACT Frequencies of multilocus male gametes in seeds collected from clones in several blocks of a white spruce seed orchard were analyzed as part of a 2-yr study of mating system variation in this species. Observed frequencies of male gamete types departed significantly from those expected assuming equal male fertilities among clones. Male gamete frequencies in seed crops were significantly heterogeneous among clones within blocks, and among blocks within clones. Clonal male fertilities were estimated from male gamete frequency data. These estimates were highly skewed, with a small proport

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6

Viramontes, F., F. Filion, and L. C. Smith. "5 NEUTRAL SEGREGATION OF DONOR CELL MITOCHONDRIA IN FETAL AND ADULT TISSUES OF SOMATIC CELL CLONES IN CATTLE." Reproduction, Fertility and Development 17, no. 2 (2005): 153. http://dx.doi.org/10.1071/rdv17n2ab5.

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Until now, animal cloning has been extremely inefficient: only 1–2% of nuclear transfer (NT) clones survive to birth. Some of these anomalies may be related to an incompatibility between nuclear and mitochondrial genes (Cummins JM 2001 Hum. Reprod. Update 7, 217–228). Controversy exists as to the levels of donor cell mitochondrial DNA (mtDNA) inheritance in somatic clones (heteroplasmy). Whereas some researchers found very low quantities (0.1–4%) (Steinborn R et al. 2000 Nat. Genet. 25, 255–257), others found levels of heteroplasmy ranging from 6 to 40% (Takeda et al. Mol. Reprod. Dev. 64, 429

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7

Hansen, O. K., and E. D. Kjær. "Paternity analysis with microsatellites in a Danish Abies nordmanniana clonal seed orchard reveals dysfunctions." Canadian Journal of Forest Research 36, no. 4 (2006): 1054–58. http://dx.doi.org/10.1139/x05-299.

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A paternity analysis using five microsatellite markers was conducted in a Danish clonal seed orchard with 13 Abies nordmanniana (Stev.) Spach clones. The purpose was to investigate potential seed-orchard dysfunctions, with special emphasis on nonequal pollen contributions and selfing. Male paternity was found for 232 seedlings germinated from seeds collected on three ramets, each of eight clones, and the relative contribution of each clone to the gene pool of male gametes was calculated. Furthermore, 49 ramets were genotyped to check for erroneous grafting. The effect of an unbalanced male con

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8

Karim, S. Abdul, and B. J. Hawkins. "Variation in response to nutrition in a three-generation pedigree of Populus." Canadian Journal of Forest Research 29, no. 11 (1999): 1743–50. http://dx.doi.org/10.1139/x99-149.

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Response to nitrogen (N) and phosphorus (P) was examined in a three-generation pedigree of Populus in 1996 and 1997. In 1996, the first-generation parental clones female Populus trichocarpa Torr. & Gray and male Populus deltoides Bartr. and second-generation male and female F1 hybrids were grown in five treatments of varying N:P ratio. In 1997, 29 third-generation F2 clones and the first-generation clones were grown with high or low N and P. Variability in response to nutrients existed among and within generations. Growth increased with N supply but not with P supply because of N limitatio

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9

Kang, Kyu-Suk. "Clonal and annual variation of flower production and composition of gamete gene pool in a clonal seed orchard of Pinus densiflora." Canadian Journal of Forest Research 30, no. 8 (2000): 1275–80. http://dx.doi.org/10.1139/x00-060.

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The numbers of female and male strobili were assessed for 99 clones in a Pinus densiflora Sieb. et Zucc. seed orchard for four consecutive years. The data showed that differences for the production of both female and male strobili among clones were very large and statistically significant for all years examined. The most abundant quartile of clones produced 48.1 and 37.3% of the total female and male strobili, respectively, averaged over the 4-year period. Differences in female strobilus production among clones were more variable than in male. Pearson's correlations between years for flower pr

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10

Alexander, Lisa W., and Keith E. Woeste. "Phenology, dichogamy, and floral synchronization in a northern red oak (Quercus rubra) seed orchard." Canadian Journal of Forest Research 46, no. 5 (2016): 629–36. http://dx.doi.org/10.1139/cjfr-2015-0312.

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We developed a novel scoring system to assess spring phenology in a northern red oak (Quercus rubra L.) clonal seed orchard. The system was used to score from 304 to 364 ramets for three reproductive seasons and to place clones into early, intermediate, and late phenology classes. Although the absolute number of clones in each phenological class changed from year to year, the overall order of clonal flowering was highly stable (rs = 0.67, p < 0.001). Early-flowering clones flowered significantly longer than later flowering clones in all 3 years. Dichogamy was present in the orchard, with ma

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11

Muñoz-Gutiérrez, Liliana, J. Jesús Vargas-Hernández, Javier López-Upton, Carlos Ramírez-Herrera, and Marcos Jiménez-Casas. "Clonal variation in phenological synchronization and cone production in a Pinus patula seed orchard." Silvae Genetica 69, no. 1 (2020): 130–38. http://dx.doi.org/10.2478/sg-2020-0018.

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Abstract Synchronization between female receptiveness and pollen dispersal, and fecundity of clones influence effective population size and genetic diversity of germplasm produced in seed orchards. Our objective was to determine clonal variation in phenological synchronization and in cone production in a Pinus patula seed orchard. Two-year phenology data of female and male strobili from a sample of 31 clones, and of male strobili in trees from neighboring natural stands of the same species were used. Synchronization indices between female receptiveness and pollen release of the same clone (O̅i

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12

de Boer, I. J. M., T. H. E. Meuwissen, and J. A. M. van Arendonk. "Combining the genetic and clonal responses in a closed dairy cattle nucleus scheme." Animal Science 59, no. 3 (1994): 345–58. http://dx.doi.org/10.1017/s000335610000787x.

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AbstractDesigns testing clones in a closed nucleus, in which 1024 cows are tested each year, were compared for their additive genetic response to selection (genetic response) and their genetic superiority of female genotype(s) selected for commercial cloning (clonal response), using stochastic simulation. Clones were tested at the expense of dam or sire families, matings per dam (sire), or full-sibs per family. The reference design maximized the genetic response corrected for inbreeding in the absence of cloning. The trait considered was overall economic merit for milk production, which was si

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13

Tolaini, V., A. Ciancolini, P. Crinò, L. Capuzzi, and C. Bastioli. "Development of cardoon male sterile clones." Acta Horticulturae, no. 1284 (June 2020): 47–50. http://dx.doi.org/10.17660/actahortic.2020.1284.5.

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14

Ashfaq, A. Mir, H. Masoodi T., A. Mir Naseer, A. Rather T., and A. Sofi P. "Nursery Performance of Male Clones of Poplar (Populus deltoides Bartr.) under Temperate Conditions of Kashmir Valley." British Journal of Applied Science & Technology 21, no. 1 (2017): 1–8. https://doi.org/10.9734/BJAST/2017/32669.

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Fifteen (15) male clones of <em>Populus deltoides</em> (Twelve clones procured from WIMCO and three local clones) were evaluated in nursery to study their growth performance. The investigations revealed that significant interclonal variation exists for all the selected growth characters. The plant height varied from 179 to 284 cm, collar diameter 17.13 to 27.51 mm, number of branches 3 to 14 and number of leaves 59 to 272 plant<sup>-1</sup>. The leaf area plant<sup>-1</sup> also varied between a minimum of 50 to a maximum of 118 cm2 and the internodal length between 3.28 to 5.77 cm. Significan

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15

Merlo, E., and J. Fernández-López. "Analysis of the parental reproductive balance in a Pinus pinaster Ait. seed orchard." Forest Systems 13, no. 2 (2004): 387–97. http://dx.doi.org/10.5424/841.

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The number of female and male strobili were assessed for 22 clones in a Pinus pinaster Ait. seed orchard along four consecutive years. The data showed that differences for the production of both female and male strobili among clones were very large and statistically significant for all years examined. Differences in female strobili production among clones were more variable than in male strobili. Pearson’ s correlations between years were highly significant. Both correlations between female and male flowers were moderate and positive for all 4 years. Broad-sense heritabilities for flower produ

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16

Panahandeh, J. "Dihaploid induction ability of three clones of Solanum phureja (2n = 2x = 24) in interploidy cross with S. tuberosum (2n = 4x = 48)." Acta Agronomica Hungarica 58, no. 1 (2010): 49–54. http://dx.doi.org/10.1556/aagr.58.2010.1.6.

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Potato, Solanum tuberosum L. (2n = 4x = 48), is an autotetraploid species, the breeding of which at the tetraploid level is complicated by tetrasomic inheritance. Dihaploids (2n = 2x = 24) from the tetraploid cultivated potato have great potential for breeding and genetic studies. The common method deployed to obtain potato dihaploids is to make interspecific-interploidy (4x × 2x) crosses between a tetraploid seed parent and special clones from the diploid S. phureja as pollinator. Pollinators carrying a marker gene have been used, but unfortunately, these clones were very weak, with rare flow

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17

Ström, Anders, Agneta Mode, Peter Zaphiropoulos, Anne-Gerd Nilsson, Edward Morgan та Jan-Åke Gustafsson. "Cloning and pretranslational hormonal regulation of testosterone 16α-hydroxylase (P-45016α) in male rat liver". Acta Endocrinologica 118, № 2 (1988): 314–20. http://dx.doi.org/10.1530/acta.0.1180314.

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Abstract. cDNA clones for P-45016α were isolated from a male liver λgt 11 expression library using antibodies against P-45016α. The clones encompassed 1633 and 1791 bp, respectively. The latter clone contained the whole coding sequence. The 20 deduced NH2-terminal amino acids were identical to those of P-450h and the cDNA sequence was in complete agreement with that of P-450 (M-1). Northern blots showed that P-45016α in the rat liver is pretranslationally regulated by the growth hormone secretory pattern. Southern blots indicated that few genes belong to the same P-450 gene family as P-45016α.

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18

Tschaplinski, T. J., G. A. Tuskan, and C. A. Gunderson. "Water-stress tolerance of black and eastern cottonwood clones and four hybrid progeny. I. Growth, water relations, and gas exchange." Canadian Journal of Forest Research 24, no. 2 (1994): 364–71. http://dx.doi.org/10.1139/x94-049.

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Water-stress tolerance of six clones in a pedigree consisting of black cottonwood (Populustrichocarpa Torr. & Gray, female) and eastern cottonwood (Populusdeltoides Bartr., male) parental clones and four hybrid progeny was investigated. Trees were grown outdoors in pots; well-watered trees were kept moist (soil water potential greater than −0.03 MPa), and stressed trees (soil water potential less than −2.0 MPa) were subjected to repeated cyclical stress of 1 or 2 days duration over the 14-week study. Male P. deltoides and the male clone 242 displayed the greatest degree of stress tolerance

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19

Kato, Kazutaka. "Variation in the amount of pollen per male flower on Abies sachalinensis." Silvae Genetica 73, no. 1 (2024): 1–12. http://dx.doi.org/10.2478/sg-2024-0001.

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Abstract It is important to evenly increase the amount of scattered pollen per clone for maintaining high genetic variation in clonal seed orchards. It is thus imperative to determine not only the number of male flowers per tree, but also the amount of pollen per male flower in each clone. In this study, the existence of annual variation on the fresh weight of male flowers (FW) and the clonal and annual variation, and ramets’ variation with the same or different tree ages on the weight of pollen per male flower (WP) were investigated using 21 Abies sachalinensis clones for 3 years. The results

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20

Slade, Andrew J., and Michael J. Hutchings. "Within- and between-population variation in ramet behaviour in the gynodioecious clonal herb, Glechoma hederacea (Labiatae)." Canadian Journal of Botany 67, no. 3 (1989): 633–39. http://dx.doi.org/10.1139/b89-085.

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Clones of the gynodioecious herb Glechoma hederacea L. produce ramets which flower and bear seed, ramets which flower but fail to bear seed, and sterile ramets. Performance and survivorship of these subsets of ramets were compared for hermaphrodite and male sterile clones in contrasting grassland and woodland habitats for 2 consecutive years. For all clones, ramet densities on given dates were comparable between years. Winter survival of ramets exceeded 95% and mortality during the growing season was exponential. No genet recruitment was recorded throughout the study. For all clones, flowering

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21

Murphy, Maria. "Voicing the Clone: Laurie Anderson and Technologies of Reproduction." Feminist Review 127, no. 1 (2021): 56–72. http://dx.doi.org/10.1177/0141778920973023.

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In the 1980s, new reproductive technologies such as in vitro fertilisation and embryo transfer became commercially available in the United States, and somatic cell nuclear transfer—the cloning process by which Dolly the Sheep would be conceived in 1996—was in its experimental phase. While anxieties concerning these new technologies escalated in the popular sensorium, Laurie Anderson explored the phenomenon of cloning in a short musical film called What You Mean We? (1986) in which Anderson consults a design team to clone herself in order to manage her demanding workload. The videographic image

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22

Ertekin, M. "Clone Fertility and Genetic Diversity in a Black Pine Seed Orchard." Silvae Genetica 59, no. 1-6 (2010): 145–50. http://dx.doi.org/10.1515/sg-2010-0017.

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AbstractClonal variation in the number of female and male strobili was studied for three consecutive years (2002-2004) in a clonal black pine seed orchard in Turkey. The data showed large differences in female and male fertility among clones in the three years. The correlation between female and male strobilus production was negative and statistically significant in 2004, a good-flowering year. Clonal fertility and fertility variation, expressed by the sibling coefficient and coefficient of variation in strobilus production among 30 clones, were reported. Fertility varied among clones and amon

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23

Yulaikah, Sri, Djumali Djumali, Anik Herwati, et al. "Clone Characteristics Of Sil 04, 6535, Ps 881, And Bululawang Polycross." International Journal of Science, Technology & Management 4, no. 2 (2023): 379–85. http://dx.doi.org/10.46729/ijstm.v4i2.762.

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Indonesia’s sugar productivity is low and requires improvement. Producing new superior sugar varieties may increase sugar productivity. In 2019, a polycross between female SIL 04 and male 6535, PS 881, and Bululawang made 11 potential clones. Therefore, it is necessary to determine each clone's characteristics. This research aimed to determine superior clones that generate higher crystal production than the female parents. The research location was the Agricultural Technology Research and Assessment Installation (Instalasi Penelitian dan Pengkajian Teknologi Pertanian - IP2TP) Karangploso from

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24

Arat, Sezen, Arzu T. Caputcu, Tolga Akkoc, et al. "Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle." Reproduction, Fertility and Development 23, no. 8 (2011): 1012. http://dx.doi.org/10.1071/rd11026.

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The aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs o

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25

Held, W. A., J. F. Gallagher, C. M. Hohman, N. J. Kuhn, B. M. Sampsell, and R. G. Hughes. "Identification and characterization of functional genes encoding the mouse major urinary proteins." Molecular and Cellular Biology 7, no. 10 (1987): 3705–12. http://dx.doi.org/10.1128/mcb.7.10.3705-3712.1987.

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Mouse Ltk- cells were stably transfected with cloned genes encoding the mouse major urinary proteins (MUPs). C57BL/6J MUP genomic clones encoding MUP 2 (BL6-25 and BL6-51), MUP 3 (BL6-11 and BL6-3), and MUP 4 (BL6-42) have been identified. In C57BL/6J mice, MUP 2 and MUP 4 are known to be synthesized in male, but not female, liver, and MUP 3 is known to be synthesized in both male and female liver and mammary gland. A BALB/c genomic clone (BJ-31) was shown to encode a MUP that is slightly more basic than MUP 2 and was previously shown to be synthesized in both male and female liver of BALB/c b

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26

Held, W. A., J. F. Gallagher, C. M. Hohman, N. J. Kuhn, B. M. Sampsell, and R. G. Hughes. "Identification and characterization of functional genes encoding the mouse major urinary proteins." Molecular and Cellular Biology 7, no. 10 (1987): 3705–12. http://dx.doi.org/10.1128/mcb.7.10.3705.

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Mouse Ltk- cells were stably transfected with cloned genes encoding the mouse major urinary proteins (MUPs). C57BL/6J MUP genomic clones encoding MUP 2 (BL6-25 and BL6-51), MUP 3 (BL6-11 and BL6-3), and MUP 4 (BL6-42) have been identified. In C57BL/6J mice, MUP 2 and MUP 4 are known to be synthesized in male, but not female, liver, and MUP 3 is known to be synthesized in both male and female liver and mammary gland. A BALB/c genomic clone (BJ-31) was shown to encode a MUP that is slightly more basic than MUP 2 and was previously shown to be synthesized in both male and female liver of BALB/c b

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Chaux, Pascal, Rosalie Luiten, Nathalie Demotte, et al. "Identification of Five MAGE-A1 Epitopes Recognized by Cytolytic T Lymphocytes Obtained by In Vitro Stimulation with Dendritic Cells Transduced with MAGE-A1." Journal of Immunology 163, no. 5 (1999): 2928–36. http://dx.doi.org/10.4049/jimmunol.163.5.2928.

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Abstract MAGE genes are expressed by many human tumors of different histological types but not by normal cells, except for male germline cells. The Ags encoded by MAGE genes and recognized by T cells are therefore strictly tumor-specific. Clinical trials involving therapeutic vaccination of cancer patients with MAGE antigenic peptides or proteins are in progress. To increase the range of patients eligible for therapy with peptides, it is important to identify additional MAGE epitopes recognized by CTL. Candidate peptides known to bind to a given HLA have been used to stimulate T lymphocytes in

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28

Goto, Susumu, A. Watanabe, F. Miyahara, and Y. Mori. "Reproductive Success of Pollen Derived From Selected and Non-Selected Sources and its Impact on the Performance of Crops in a Nematode-Resistant Japanese Black Pine Seed Orchard." Silvae Genetica 54, no. 1-6 (2005): 69–76. http://dx.doi.org/10.1515/sg-2005-0011.

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Abstract The reproductive success of pollen derived from selected and non-selected sources and its impact on the performance of orchard crops were evaluated, using five pairs of microsatellite markers, in a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 nematode-resistant clones. The paternity of each open-pollinated seed was determined by comparing the genotypes of seeds from six clones (24 trees) with genotypes of the 16 orchard clones and two trees (N1, N2) representing other genotypes that had been inadvertently included in the orchard. Out of 384 seeds e

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Wu, Yongzhong, Lomas Tulsieram, Quangzhou Tao, Hong-Bin Zhang, and Steven J. Rothstein. "A binary vector-based large insert library for Brassica napus and identification of clones linked to a fertility restorer locus for Ogura cytoplasmic male sterility (CMS)." Genome 43, no. 1 (2000): 102–9. http://dx.doi.org/10.1139/g99-104.

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We constructed and characterized a large DNA insert library for Brassica napus that would facilitate genome-related research and map-based cloning efforts in Brassica species. This library, consisting of 92 160 clones arrayed in 384-well microtiter dishes, was based on a conventional plant transformation vector (binary vector), and was constructed using a single ligation with transformation efficiency of over 5000 recombinants per microliter of ligation mixture. Every clone in this library contains an insert in the size range of 30-190 kb, facilitating both chromosome walking and plant transfo

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30

Oh, H. J., M. K. Kim, G. Jang, et al. "48 THE AMOUNT OF TELOMERIC DNA IN LYMPHOCYTES OF DOG CLONES." Reproduction, Fertility and Development 20, no. 1 (2008): 104. http://dx.doi.org/10.1071/rdv20n1ab48.

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Controversy regarding the restoration of eroded telomere length of donor cells after the nuclear transfer process has arisen from previous studies of cloned cattle, mice, and pigs. Little is known about telomere lengths in dogs from either natural breeding or somatic cell nuclear transfer (SCNT). In this study, we investigated the amount of telomeric DNA (ATD) in the lymphocytes of growing dog clones and their somatic cell donors. One cloned male Afghan hound dog [Snuppy (Lee et al. 2005 Nature 436, 641)] and 3 cloned female Afghan hound dogs (Jang et al. 2006 Theriogenology; doi:10.1016J.THER

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31

Oh, Dae-Geun, Ki Taek Kim, and Hyeong-Do Kim. "SEARCH FOR THE FERTILE GARLIC CLONES AND THE PERFORMANCE OF HYBRIDS BETWEEN FERTILE CLONES." HortScience 27, no. 6 (1992): 611f—611. http://dx.doi.org/10.21273/hortsci.27.6.611f.

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To find fertile garlic clones to be used in the cross breeding program, 213 accessions were subjected to cytological study at meiosis I. Most accessions (185 clones) showed abnormal chromosome configurations of ring of four, six or eight, which are ascribed to single or multiple translocations. Two clones showed asynaptic behavior forming 16 univalents from zygotene to metaphase I, and 26 clones revealed regular chromosome paring of 8 bivalents. Only six of 26 clones showing normal meiosis produced viable pollen grains, while the rest were male sterile. From the segregation of crosses between

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Kang, K. S., C. S. Kim, and Y. A. El-Kassaby. "Clonal Variation in Acorn Production and its Effect on the Effective Population Size in a Quercus acutissima Seed Orchard." Silvae Genetica 59, no. 1-6 (2010): 170–75. http://dx.doi.org/10.1515/sg-2010-0020.

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Abstract Acorn production was surveyed for eight consecutive years (2000-2007) in a 94-clone Sawtooth oak (Quercus acutissima) seed orchard established in 1992. Acorn production commenced in 2000 and peaked in 2005 and was characterized by a 3-4 years interval. Sixty out of the orchard’s 94 clones were consistent producers across the study period. Acorn production’s Pearson productmoment and Spearman rank correlation coefficients were significant and consistently positive over the eight years study period. Parental cumulative reproductive output, represented by parental balance curves, slightl

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Bachiller, Daniel, and Lucas Sánchez. "Production of X0 clones in XX females of Drosophila." Genetical Research 57, no. 1 (1991): 23–28. http://dx.doi.org/10.1017/s0016672300028998.

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SummaryThe experiments reported here are aimed at determining whether mutations deleting the function of the Sex-lethal (Sxl) gene are able to suppress the lethality of X0 clones, induced in females after the time when the state of activity of Sxl is irreversibly fixed by the ratio of the number of X chromosomes to sets of autosomes (X: A). This analysis was carried out by comparing the frequency of induced male clones (X0 constitution) in SxlfLS/ + and Sxl+/Sxl+ females, following irradiation at blastoderm and larval stages. The genotype used in these experiments, however, could also give ris

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Moriguchi, Y., S. Tsuchiya, H. Iwata, et al. "Factors Influencing Male Reproductive Success in a Cryptomeria japonica Seed Orchard Revealed by Microsatellite Marker Analysis." Silvae Genetica 56, no. 1-6 (2007): 207–14. http://dx.doi.org/10.1515/sg-2007-0031.

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AbstractWe investigated the influence of male flower production, floral synchrony and inter-tree distances on male reproductive success in a miniature seed orchard of Cryptomeria japonica. We used six microsatellite markers to determine the paternity of each seed. In the seed orchard, the average pollen contamination and clonal self-fertilization rates were 38.7% and 1.7%, respectively. The level of male reproductive success of constituent clones varied from 0.0 to 15.7%. Five clones showing the highest male reproductive success contributed ca. 30% of all analyzed seeds as a pollen donor after

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Wuriandani, Adinda, Sobir Sobir, Desta Wirnas, and Agung Wahyu Susilo. "Genetic components estimation of F1 population of cocoa (Theobroma cacao L.) in drought stress condition." Pelita Perkebunan (a Coffee and Cocoa Research Journal) 35, no. 2 (2019): 85–93. http://dx.doi.org/10.22302/iccri.jur.pelitaperkebunan.v35i2.376.

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Drought stress is a major constraint in cocoa production. The use of drought tolerant clone is the most efficient tool to overcome drought problem in cocoa production. North Carolina II (NCII) mating-design was used to identify parental and progeny performance in drought stress. The crossing consisted of three female parent clones namely KW 516, Sulawesi 3, and TSH 858, while male parent clones were ICCRI 09 and Scavina 6 then produced 6 combinations crosses. Genotypes used were 11 genotypes consisted of 5 parent clones and 6 cross genotypes. Drought treatment was applied to cocoa seedlings at

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Kuribayashi, K., C. Tanaka, Y. Matsubayashi, et al. "Anti-idiotypic antibodies against UV-induced tumor-specific CTL clones. Preparation in syngeneic combination." Journal of Immunology 141, no. 11 (1988): 4074–80. http://dx.doi.org/10.4049/jimmunol.141.11.4074.

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Abstract In this study, we first established several CTL clones of (BALB/c x C57BL/6)F1 origin that were specific for either syngeneic UV female 1 or UV male 1 fibrosarcoma cell lines. All the CTL clones had Thy-1+ Lyt-2+ L3T4- phenotypes and showed Kd restriction when lysing the corresponding target cells. Sera obtained from syngeneic animals immunized with three CTL clones, 10B-5 for UV female 1, and CTL9 and CTL10 for UV male 1, showed specific inhibition of target cell lysis with the corresponding CTL clones. The inhibitory activities were found in sera of the majority of immunized animals

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Goto, S., F. Miyahara, and Y. Ide. "Monitoring male reproductive success in a Japanese black pine clonal seed orchard with RAPD markers." Canadian Journal of Forest Research 32, no. 6 (2002): 983–88. http://dx.doi.org/10.1139/x02-022.

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Overall male reproductive success was investigated in a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 nematode-resistant clones, using random amplified polymorphic DNA (RAPD) markers. We genotyped all 16 of the orchard clones at selected RAPD loci by analyzing diploid needles and 10 haploid megagametophytes from each of them. In addition, the male parent of open-pollinated seedlings was identified by comparing their phenotypes with the genotypes of the orchard clones. In this way the male parents of 559 seedlings of 648 seedlings of progenies from 14 parenta

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Yuniastuti, Endang. "Analisis Fenotipik dan Genotipik pada Abnormalitas Klon-klon Kelapa Sawit (Elaeis guineensis Jacq.) Hasil Kultur Jaringan." Caraka Tani: Journal of Sustainable Agriculture 22, no. 1 (2018): 1. http://dx.doi.org/10.20961/carakatani.v22i1.20524.

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<p>Research to analyze phenotype and genotype of oil palm clone developed from tissue culture which showed normal fruits and abnormal fruits through SDS-PAGE protein technique, and RAPD technique. Result indicated that protein with molecular weight of 100 kDa could differentiate genotype with normal fruit and abnormal fruit from all clones observed. Study on DNA through RAPD technique revealed primers which differentiated genotypes (male flowered genotype, normal fruited genotype, and abnormal fruited genotype) within clone and interclonal, i.e. OPC-07, OPN-16, and SC 10-56. Molecular we

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Bell, Daniel J., Frank A. Drummond, and Lisa J. Rowland. "Evidence of functional gender polymorphisms in a population of the hermaphroditic lowbush blueberry (Vaccinium angustifolium)1This article is part of a Special Issue entitled “A tribute to Sam Vander Kloet FLS: Pure and applied research from blueberries to heathland ecology”." Botany 90, no. 5 (2012): 393–99. http://dx.doi.org/10.1139/b11-064.

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Lowbush blueberry ( Vaccinium angustifolium Aiton) is a wild, but cultivated for profit, agricultural system grown primarily in the northeastern regions of North America. It has been suggested that a functional gender specialization may be occurring in this hermaphroditic species in which some individuals (termed clones) effectively function as bearers (high ovule production but little or nonviable pollen) or as pollen donors (low ovule number with high quantities of viable pollen). The purpose of this study was to investigate if functional gender specialization was occurring in a randomly cho

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Nishioka, Yutaka, and Estelle Lamothe. "Evolution of a mouse Y chromosomal sequence flanked by highly repetitive elements." Genome 29, no. 2 (1987): 380–83. http://dx.doi.org/10.1139/g87-065.

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Mammalian primary sex is determined by the presence or absence of the Y chromosome. However, little is known about the molecular processes through which the Y chromosome exerts its action. We applied recombinant DNA techniques to isolate mouse Y chromosomal fragments and described previously a clone designated as AC11 (Y. Nishioka and E. Lamothe. 1986. Genetics, 113: 417–432). To obtain information on DNA sequences that flank AC11, we screened a mouse genomic library for the presence of AC11-related sequences and isolated over 50 positive clones. In this report we describe clones ACC2 and ACC3

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Codesido, V., E. Merlo, and J. Fernández-López. "Variation in Reproductive Phenology in a Pinus radiata D. Don Seed Orchard in Northern Spain." Silvae Genetica 54, no. 1-6 (2005): 246–56. http://dx.doi.org/10.1515/sg-2005-0035.

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Summary Reproductive phenology was studied in a Pinus radiata seed orchard, located in northern Spain. Timing of flowering was determined on the basis of data recorded by visual observations made in 2000, 2001 and 2002. The genetic and environmental factors affecting female and male phenology, as well as reproductive synchronization, were studied. The dates of beginning of the receptive phase and pollen shedding varied greatly from year to year but the variation on the sum of degree-days was low. In general, the flowering periods of the different clones overlapped. The clonal differences in th

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Ivanov, Roman, Samantha Hol, Tineke Aarts, Anton Hagenbeek, and Saskia Ebeling. "Generation of Graft-Versus-Leukemia (GVL)-Effector T Cells through Targeting of Leukemia-Associated HY Antigens Via TCR Transfer." Blood 104, no. 11 (2004): 2117. http://dx.doi.org/10.1182/blood.v104.11.2117.2117.

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Abstract In this study we present two novel leukemia-associated antigens and their specific targeting through TCR-transfer. First, two CD8+ CTL clones were characterized that specifically recognized normal and malignant male B and T cell lymphoblasts, but not fibroblasts, resting lymphocytes or monocytes. One of clones recognized the HLA-B*5201-restricted TIRYPDPVI epitope of the RPS4Y protein, while the other recognized the HLA-B*5201-restricted MQQMRHKEV epitope of the UTY protein. Western blot analysis demonstrated the overexpression of RPS4 proteins in normal and malignant lymphoblasts. Ho

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Fattizzo, Bruno, Alan Dunlop, Robin M. Ireland, et al. "Prognostic and Predictive Impact of Small PNH Clones in a Large Cohort of Patients with Myelodysplastic Syndromes and Aplastic Anemia: A Single-Center Experience." Blood 132, Supplement 1 (2018): 3870. http://dx.doi.org/10.1182/blood-2018-99-116772.

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Abstract Background: the use of high sensitive FLAER in the last 10 years improved the detection of very small PNH clones (<1%) in various hematologic conditions, including aplastic anemia (AA)/myelodysplastic syndromes (MDS). Although generally considered unremarkable, the recent observation of asymptomatic end-organ damage due to undiagnosed thrombosis even in patients without overt hemolysis questions the assertion that small PNH clones are of subclinical value. Aim: To evaluate the prevalence of PNH clones ≥0.01% in AA/MDS patients tested at a single tertiary center, and to assess their

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Novak, Ben J., Oliver A. Ryder, Marlys L. Houck, et al. "Endangered Przewalski’s Horse, Equus przewalskii, Cloned from Historically Cryopreserved Cells." Animals 15, no. 5 (2025): 613. https://doi.org/10.3390/ani15050613.

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Cloning from historically cryopreserved cells offers a potential means to restore lost genetic variation or increase the representation of particular lineages within bottlenecked species, provided such biobanked materials are archived for such genetic rescue applications. One species for which cloning can provide genetic management benefits is Przewalski’s horse, Equus przewalskii. All ~1800 living Przewalski’s horses, distributed across ex situ breeding facilities and in situ reintroduction sites, are descended from one or more of the five founder lineages established by the 12 horses capture

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HAMADINA, E. I., P. Q. CRAUFURD, and R. ASIEDU. "Flowering intensity in white yam (Dioscorea rotundata)." Journal of Agricultural Science 147, no. 4 (2009): 469–77. http://dx.doi.org/10.1017/s0021859609008697.

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SUMMARYWhite or Guinea yam (Dioscorea rotundata), grown for its underground tubers, is an important food in West Africa. Progress in yam breeding is constrained by variable flowering behaviour, making hybridization difficult. Yam clones may be dioecious, monoecious or hermaphrodite with variable sex ratios. The proportion of plants that flower and the flowering intensity also vary with season and location. The objective of the present work was to investigate whether variation in flowering behaviour was related to factors determining rate of development (photoperiod and temperature through sowi

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Costa, Natalia S., Márcia M. Silveira, Luna N. Vargas, Alexandre R. Caetano, Rodolfo Rumpf, and Maurício M. Franco. "Epigenetic characterization of the H19/IGF2 locus in calf clones placenta." Pesquisa Veterinária Brasileira 40, no. 12 (2020): 1063–72. http://dx.doi.org/10.1590/1678-5150-pvb-6491.

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ABSTRACT: Somatic Cell Nuclear Transfer (SCNT-Cloning) is a promising technique in many areas and is based on genetically identical individuals. However, its efficiency is low. Studies suggest that the leading cause is inadequate epigenetic reprogramming. This study aimed to characterize the methylation pattern of the exon 10 regions of the IGF2 gene and the Imprinting Control Region (ICR) of the H19 gene in the placenta of cloned calves. For this study, female and male cloned calves presenting different phenotypes were used. Genomic DNA from these animals’ placenta was isolated, then treated

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Munsterberg, A., and R. Lovell-Badge. "Expression of the mouse anti-mullerian hormone gene suggests a role in both male and female sexual differentiation." Development 113, no. 2 (1991): 613–24. http://dx.doi.org/10.1242/dev.113.2.613.

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We describe here the isolation of cDNA and genomic clones corresponding to the mouse gene encoding anti-Mullerian hormone, and the use of these clones as molecular probes to study AMH gene expression. We constructed a 14.5 days post coitum (dpc) mouse fetal testes library and isolated a cDNA clone using bovine, human and rat partial cDNAs as probes. This clone contained a 1 kb insert, which was confirmed by sequencing to be the mouse homologue of AMH. Probes derived from the mouse cDNA clone were used to screen genomic libraries and a 12 kb DNA fragment containing the complete coding region of

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Almqvist, Curt. "Interstock effects on topgraft vitality and strobili production after topgrafting in Pinus sylvestris." Canadian Journal of Forest Research 43, no. 6 (2013): 584–88. http://dx.doi.org/10.1139/cjfr-2012-0507.

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Reducing generation turnover in breeding programs should increase the genetic gain per unit time. This could potentially be achieved by topgrafting, since it can be used to induce strobili production in young plant material. I therefore studied the effect of the interstock clone on the vitality and strobili production of topgrafts in Pinus sylvestris L. over 4 years. The experiment consisted of 20 interstock clones × 3 ramets per interstock × 10 topgrafts per ramet, giving a total of 600 topgrafts. There were large differences in topgraft survival rates among the different interstock clones, r

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Zhuowen, Z. "Studies on the Pollination Characteristics and Pollination Level of Chinese fir Seed Orchard." Silvae Genetica 53, no. 1-6 (2004): 7–11. http://dx.doi.org/10.1515/sg-2004-0002.

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Abstract Data from three seed orchards (Chongyang, Zhangle and Laoshan) and Lintian forest were used to study pollination characteristics, pollination level, pollen and ovule production in Chinese fir. The results show that male and female cones have their own distribution patterns within crowns. Male cones are located in the middle to top and female cones the middle to bottom part of the crown. Because of lower density of trees in seed orchard than that in a stand, female cones are distributed over the entire crown in seed orchard trees. Chinese fir male and female cones appear to be very wel

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Tsarev, Vadim A., Anatoly P. Tsarev, Raisa P. Tsareva, and Natal’ya V. Laur. "Long-Term Testing of Aspen Clones in European Russia." Lesnoy Zhurnal (Forestry Journal), no. 3 (June 1, 2025): 9–19. https://doi.org/10.37482/0536-1036-2025-3-9-19.

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The results of 40 years of testing of more than 20 aspen clones selected in various regions of European Russia and the USSR, growing in the Semiluki Tremuletum in the Voronezh Region, are presented. The survival of plants, growth in height, diameter and volume of the stem are shown. A ranked assessment has been given and a selection of promising clones for the creation of highly productive aspen plantations has been carried out. It has also been noted that sexual dimorphism can have different effects on aspen rot resistance in different growing conditions. The testing has been conducted at the

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