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Artykuły w czasopismach na temat "Merluccius spp"
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Cipriani, Paolo, Gian Luca Sbaraglia, Michela Paoletti, et al. "The Mediterranean European hake, Merluccius merluccius : Detecting drivers influencing the Anisakis spp. larvae distribution." Fisheries Research 202 (June 2018): 79–89. http://dx.doi.org/10.1016/j.fishres.2017.07.010.
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Oliva, Marcelo E., Leyla Cárdenas, Isabel M. Valdivia, Paulina Bruning, Luis Figueroa-Fabrega, and Rubén Escribano. "Spatial Pattern of Genetic Diversity in the Blood Fluke Aporocotyle argentinensis (Digenea, Aporocotylidae) from South American Hakes (Pisces: Merluccidae)." Diversity 14, no. 9 (2022): 772. http://dx.doi.org/10.3390/d14090772.
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Distribution of blood fluke Aporocotyle spp. parasitizing Merluccius species from the coasts of South America (Peru, Chile and Argentina) constitutes an excellent opportunity to evaluate the geographical amplitude in which a parasite can exploit the same host species. Phylogenetic analyses (partial sequences of SSU rDNA, LSU rDNa, and cox1 gene) were performed to characterize the genetic lineage of Aporocotyle species described from South American Hake: Merluccius australis, M. gayi, and M. hubbsi. The Phylogenetic analyses (SSUrDNA and LSUrDNA) revealed an absence of genetic variability in Aporocotyle obtained over a gradient of 6800 km, covering two oceans and three closely related hosts. Consequently, the species infecting Merluccius spp. in South America is Aporocotyle argentinensis Smith 1969, by priority law. Phylogeographic analysis suggests a pattern of spatial differentiation and genetic population structure associated with the geographical distribution of the host’s species. A specimen with a haplotype found in M. gayi was collected from M. australis from Puerto Montt, and three worms (from Coquimbo, Constitución and Talcahuano, host M. gayi) harbored a haplotype found in M. australis + M. hubbsi, suggesting that the gene flow between different hosts and geographical distributions occurs when the distribution of adequate hosts overlaps, avoiding speciation in blood flukes from South American hakes.
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Mladineo, Ivona, and Vedran Poljak. "Ecology and Genetic Structure of Zoonotic Anisakis spp. from Adriatic Commercial Fish Species." Applied and Environmental Microbiology 80, no. 4 (2013): 1281–90. http://dx.doi.org/10.1128/aem.03561-13.
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ABSTRACTConsumption of raw or thermally inadequately treated fishery products represents a public health risk, with the possibility of propagation of liveAnisakislarvae, the causative agent of the zoonotic disease anisakidosis, or anisakiasis. We investigated the population dynamics ofAnisakisspp. in commercially important fish—anchovies (Anisakis), sardines (Sardina pilchardus), European hake (Merluccius merluccius), whiting (Merlangius merlangus), chub mackerel (Scomber japonicus), and Atlantic bluefin tuna (Thunnus thynnus)—captured in the main Adriatic Sea fishing ground. We observed a significant difference in the numbers of parasite larvae (1 to 32) in individual hosts and between species, with most fish showing high or very highAnisakispopulation indices. Phylogenetic analysis confirmed that commercial fish in the Adriatic Sea are parasitized byAnisakis pegreffii(95.95%) andAnisakis simplexsensu stricto(4.05%). The genetic structure ofA. pegreffiiin demersal, pelagic, and top predator hosts was unstructured, and the highest frequency of haplotype sharing (n= 10) was between demersal and pelagic fish.
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Fuentes, Màrius V., Elena Madrid, Clara Cuesta, et al. "Anisakid Nematodes and Potential Risk of Human Anisakiasis through the Consumption of Hake, Merluccius spp., Sold Fresh in Spanish Supermarkets." Pathogens 11, no. 6 (2022): 622. http://dx.doi.org/10.3390/pathogens11060622.
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Nematode parasite species belonging to the Anisakis simplex complex are the most important cause of human anisakiasis through the consumption of (mainly) undercooked, previously not frozen, or conveniently treated fish. In Spain, the consumption of hake has been recognized as an important source of this parasitosis. With the aim of shedding light on the risk factors that can influence the potential risk of human anisakiasis in Spain through the consumption of fresh hake sold by nationwide supermarket chains, a total of 536 small hake specimens belonging to the species Merluccius bilinearis caught off the Northeast American coasts and Merluccius merluccius caught in the Northeast Atlantic and Mediterranean waters was analysed. Anisakids morphologically identified as Anisakis type I were found as the most prevalent and the most abundant species and were considered the main potential cause of human anisakiasis. Intrinsic and extrinsic factors concerning the hake, such as its origin and season of capture, its size, as well as the days passed between its capture and consumption, should be taken into account to avoid this human parasitosis. It is essential that consumers have access to fish label information which should include, as regulated by the European Commission, traceability data.
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Ramilo, Andrea, Helena Rodríguez, Santiago Pascual, Ángel F. González, and Elvira Abollo. "Population Genetic Structure of Anisakis simplex Infecting the European Hake from North East Atlantic Fishing Grounds." Animals 13, no. 2 (2023): 197. http://dx.doi.org/10.3390/ani13020197.
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The European hake, one of the most commercially valuable species in ICES fishing areas, is considered an important neglected source of zoonotic risk by nematode parasites belonging to the genus Anisakis. Merluccius merluccius is, by far, the most important host of Anisakis spp. at the European fishing grounds, in terms of demographic infection values, and carries the highest parasite burden. These high parasite population densities within an individual fish host offer a chance to explore new sources of variations for the genetic structure of Anisakis spp. populations. A total of 873 Anisakis spp. third-stage larvae, originally sampled from viscera and muscular sections of hake collected at ten fishing grounds, were primarily identified using ITS rDNA region as molecular marker. After that, we used mtDNA cox2 gene to reveal the high haplotype diversity and the lack of genetic structure for A. simplex. Dominant haplotypes were shared among the different fishing areas and fish sections analyzed. Results indicate a clear connection of A. simplex from European hake along the Northern North Sea to the Portuguese coast, constituting a single genetic population but revealing a certain level of genetic sub-structuring on the Northwest coast of Scotland. This study also provides useful information to advance the understanding of parasite speciation to different fish host tissues or microenvironments.
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Santos, Maria J., Matilde Matos, Lisa Guardone, et al. "Preliminary Data on the Occurrence of Anisakis spp. in European Hake (Merluccius merluccius) Caught Off the Portuguese Coast and on Reports of Human Anisakiosis in Portugal." Microorganisms 10, no. 2 (2022): 331. http://dx.doi.org/10.3390/microorganisms10020331.
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Parasitic nematodes of the genus Anisakis are among the most important biological hazards associated with seafood. A survey of Anisakis spp. in European hake (Merluccius merluccius) was undertaken as this species is a staple of the Portuguese diet. Moreover, a literature review of cases of anisakiosis reported from Portugal, a country with one of the highest levels of fish consumption in the world, was also carried out. Seventy-five European hake caught in the Atlantic Ocean off the northern coast of Portugal were analyzed to determine the infection levels and site distribution of Anisakis spp. Isolated nematode larvae were identified to species level by molecular analysis. Two sets of samples were collected. Firstly, a total of 46 Anisakis spp. L3 larvae were collected with a prevalence of 76.7% (95% CI 61.5–91.8%) and intensity (mean ± SD, range) of 2.0 ± 1.2 (1–5). Most larvae were found on the liver (45.7%) and on the gonads (32.6%), but none in the muscle. The molecular analysis showed the presence of both A. simplex s.s. (70%) and A. pegreffii (30%). For the second sample, analyzed using the UV-Press method, a total of 473 Anisakis spp. were found, with a prevalence of 95.6% (95% CI 89.5–100.0%), intensity (mean ± SD, range) of 11.3 ± 9.7 (1–41), density of 0.05 ± 0.04 (0–0.16) worms/muscle weight in g, and density of 0.54 ± 0.50 (0–2.53) worms/viscera weight in g. Surprisingly, only three very recent cases of human anisakiosis in Portugal have been reported in the literature. Data from this study contribute towards an updating of the existing epidemiological picture in an area characterized by very high seafood consumption and changing eating habits.
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Garcia-de-Vinuesa, Alfredo, Iván Sola, Federico Quattrocchi, Francesc Maynou, and Montserrat Demes. "Linking trawl fleet dynamics and the spatial distribution of exploited species can help to avoid unwanted catches: the case of the NW Mediterranean fishing grounds." Scientia Marina 82, S1 (2018): 165. http://dx.doi.org/10.3989/scimar.04755.17a.
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With the full implementation of the landing obligation on 1 January 2019, In European waters it will become mandatory for the trawling fleet to land at port all catches of certain species because, according to Article 15 of the new European Common Fisheries Policy, the species subject to the minimum conservation reference size (MCRS) cannot be discarded. Additionally, since 2005, trawlers over 15 m in length are required to carry an onboard vessel monitoring system (VMS), which generates information on fleet dynamics. The objective of this work was to provide a tool for avoiding unwanted catches by integrating the catch study of trawlers operating in the port of Blanes together with VMS data. To achieve this objective, the catches of 40 hauls were monitored, sampled and analysed together with VMS data for the years 2012-2014 integrated in a geographical information system. The results show that specimens below the MCRS were often captured in crinoid aggregation habitats, bottoms with maërl and muddy bottoms that were identified as nursery habitats of commercial species, e.g. Merluccius merluccius, Pagellus spp. and Mullus spp. VMS data showed considerable fishing pressure on areas with maërl and muddy habitats during the recruitment periods of these and other commercially relevant species. Implementing spatial or seasonal closures in habitats where species regulated by the MCRS are subject to catches could be a useful tool for preventing unwanted catches.
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Caputo, M., S. Elwen, T. Gridley, et al. "Dietary plasticity of two coastal dolphin species in the Benguela upwelling ecosystem." Marine Ecology Progress Series 669 (July 8, 2021): 227–40. http://dx.doi.org/10.3354/meps13755.
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Defining the trophic relationships of marine predators and their dietary preferences is essential in understanding their role and importance in ecosystems. Here we used stable isotope analysis of skin samples (δ15N values reflecting trophic level and δ13C values reflecting foraging habitat) to investigate resource partitioning and spatial differences of the feeding ecology of dusky dolphins Lagenorhynchus obscurus and Heaviside’s dolphins Cephalorhynchus heavisidii from 2 coastal study sites separated by 400 km along the coast of central (Walvis Bay) and southern (Lüderitz) Namibia in the Benguela upwelling ecosystem. Overall, isotopic niches of both predators were significantly different, indicating partitioning of resources and foraging habitats. Despite their smaller body size, Heaviside’s dolphins fed at a significantly higher trophic level than dusky dolphins. Stable isotope mixing models revealed that both species fed on high trophic level prey (i.e. large Merluccius spp., large Sufflogobius bibarbatus, and Trachurus t. capensis) at Walvis Bay. The diet of both dolphin species included smaller pelagic fish and squid at Lüderitz. Spatial differences highlight that Heaviside’s and dusky dolphins may exhibit dietary plasticity driven by prey availability, and that they likely form distinct population segments. Important prey for both dolphin species, specifically Merluccius spp. and T. t. capensis, are the main target of trawl fisheries in the Benguela upwelling ecosystem, highlighting potential resource overlap between dolphins and fisheries.
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Luca, F., A. Vovlas, A. Troccoli, and N. Vovlas. "A polyphasic approach with molecular phylogeny for the characterization of Anisakis pegreffii (Anisakidae: Nematoda) in fishes from Adriatic Sea." Helminthologia 50, no. 2 (2013): 112–18. http://dx.doi.org/10.2478/s11687-013-0118-2.
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AbstractIn this study we investigated the morphometric and molecular characterization of a liver encapsulated third-stage larval population of Anisakis spp. infecting Merluccius merluccius and Lophius piscatorius caught in the Adriatic Sea waters (southern Italy). A polyphasic approach based on PCR-RFLP profiles of the ITS region, mitochondrial COI (cytochrome c oxidase subunit 1), sequencing and molecular phylogeny of ITS and mitochondrial COI was used to identify Anisakis larvae collected from fish samples. PCR-RFLP analysis showed three banding pattern corresponding to the peculiar pattern of A. pegreffii. Sequence data from ribosomal ITS and mitochondrial COI were analysed by Neighbour Joining, Minimum Evolution and Maximum Parsimony methods to evaluate the phylogenetic relationships among A. simplex sensu lato. The phylogenetic trees obtained for both ITS and COI revealed the existence of three distinct clades for A. simplex sensu sricto, A. simplex C and A. pegreffii and the sequences obtained in this study clearly clustered together with A. pegreffii sequences present in the database.Histopathological observations of anisakid nematode specimens detected on the liver surface of M. merluccius are illustrated. Encapsulated specimens of the L3 stage of the nematode were similar in size and morphometry to those found into the peritoneal cavity. Anisakis larvae encapsulated on the liver surface within dense and pearl coloured envelops caused host hepatic tissue necrosis, large cavities and oedematous liver spots to the host.
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Díaz-Formoso, Lara, Vanessa Silva, Diogo Contente, et al. "Antibiotic Resistance Genes, Virulence Factors, and Biofilm Formation in Coagulase-Negative Staphylococcus spp. Isolates from European Hakes (Merluccius merluccius, L.) Caught in the Northeast Atlantic Ocean." Pathogens 12, no. 12 (2023): 1447. http://dx.doi.org/10.3390/pathogens12121447.
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The indiscriminate use of antibiotics has contributed to the dissemination of multiresistant bacteria, which represents a public health concern. The aim of this work was to characterize 27 coagulase-negative staphylococci (CoNS) isolated from eight wild Northeast Atlantic hakes (Merluccius merluccius, L.) and taxonomically identified as Staphylococcus epidermidis (n = 16), Staphylococcus saprophyticus (n = 4), Staphylococcus hominis (n = 3), Staphylococcus pasteuri (n = 2), Staphylococcus edaphicus (n = 1), and Staphylococcus capitis (n = 1). Biofilm formation was evaluated with a microtiter assay, antibiotic susceptibility testing was performed using the disk diffusion method, and antibiotic resistance and virulence determinants were detected by PCR. Our results showed that all staphylococci produced biofilms and that 92.6% of the isolates were resistant to at least one antibiotic, mainly penicillin (88.8%), fusidic acid (40.7%), and erythromycin (37%). The penicillin resistance gene (blaZ) was detected in 66.6% (18) of the isolates, of which 10 also carried resistance genes to macrolides and lincosamides (mphC, msr(A/B), lnuA, or vgaA), 4 to fusidic acid (fusB), and 3 to trimethoprim-sulfamethoxazole (dfrA). At least one virulence gene (scn, hla, SCCmecIII, and/or SCCmecV) was detected in 48% of the isolates. This study suggests that wild European hake destined for human consumption could act as a vector of CoNS carrying antibiotic resistance genes and/or virulence factors.
Rozprawy doktorskie na temat "Merluccius spp"
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Milano, Ilaria <1983>. "Novel tools for conservation genetics in marine fish: population structure and evolution of the European hake (Merluccius merluccius) inferred by SNP variation and applications to traceability." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4728/1/Milano_Ilaria_tesi.pdf.
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The research presented in my PhD thesis is part of a wider European project, FishPopTrace, focused on traceability of fish populations and products. My work was aimed at developing and analyzing novel genetic tools for a widely distributed marine fish species, the European hake (Merluccius merluccius), in order to investigate population genetic structure and explore potential applications to traceability scenarios.
A total of 395 SNPs (Single Nucleotide Polymorphisms) were discovered from a massive collection of Expressed Sequence Tags, obtained by high-throughput sequencing, and validated on 19 geographic samples from Atlantic and Mediterranean.
Genome-scan approaches were applied to identify polymorphisms on genes potentially under divergent selection (outlier SNPs), showing higher genetic differentiation among populations respect to the average observed across loci. Comparative analysis on population structure were carried out on putative neutral and outlier loci at wide (Atlantic and Mediterranean samples) and regional (samples within each basin) spatial scales, to disentangle the effects of demographic and adaptive evolutionary forces on European hake populations genetic structure. Results demonstrated the potential of outlier loci to unveil fine scale genetic structure, possibly identifying locally adapted populations, despite the weak signal showed from putative neutral SNPs.
The application of outlier SNPs within the framework of fishery resources management was also explored. A minimum panel of SNP markers showing maximum discriminatory power was selected and applied to a traceability scenario aiming at identifying the basin (and hence the stock) of origin, Atlantic or Mediterranean, of individual fish. This case study illustrates how molecular analytical technologies have operational potential in real-world contexts, and more specifically, potential to support fisheries control and enforcement and fish and fish product traceability.
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Milano, Ilaria <1983>. "Novel tools for conservation genetics in marine fish: population structure and evolution of the European hake (Merluccius merluccius) inferred by SNP variation and applications to traceability." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2012. http://amsdottorato.unibo.it/4728/.
Pełny tekst źródłaStreszczenie:
The research presented in my PhD thesis is part of a wider European project, FishPopTrace, focused on traceability of fish populations and products. My work was aimed at developing and analyzing novel genetic tools for a widely distributed marine fish species, the European hake (Merluccius merluccius), in order to investigate population genetic structure and explore potential applications to traceability scenarios.
A total of 395 SNPs (Single Nucleotide Polymorphisms) were discovered from a massive collection of Expressed Sequence Tags, obtained by high-throughput sequencing, and validated on 19 geographic samples from Atlantic and Mediterranean.
Genome-scan approaches were applied to identify polymorphisms on genes potentially under divergent selection (outlier SNPs), showing higher genetic differentiation among populations respect to the average observed across loci. Comparative analysis on population structure were carried out on putative neutral and outlier loci at wide (Atlantic and Mediterranean samples) and regional (samples within each basin) spatial scales, to disentangle the effects of demographic and adaptive evolutionary forces on European hake populations genetic structure. Results demonstrated the potential of outlier loci to unveil fine scale genetic structure, possibly identifying locally adapted populations, despite the weak signal showed from putative neutral SNPs.
The application of outlier SNPs within the framework of fishery resources management was also explored. A minimum panel of SNP markers showing maximum discriminatory power was selected and applied to a traceability scenario aiming at identifying the basin (and hence the stock) of origin, Atlantic or Mediterranean, of individual fish. This case study illustrates how molecular analytical technologies have operational potential in real-world contexts, and more specifically, potential to support fisheries control and enforcement and fish and fish product traceability.