Gotowe bibliografie tematyczne / Metaphase spread

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Gotowa bibliografia na temat „Metaphase spread”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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Artykuły w czasopismach na temat "Metaphase spread"

Kotikalapudi, Rosaiah, Rajesh Patel, Hemanth Medidi, and Nagaraju Sugali. "Distribution of sex chromosomes (XY) in lymphocyte metaphase spreads of dairy bulls." Genetika 45, no. 3 (2013): 761–68. http://dx.doi.org/10.2298/gensr1303761k.

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Position of autosome and sex chromosomes in metaphase spreads is grate concerned of Cytogeneticians worldwide to understand cell biology. A few isolated studies have been conducted for the distribution of chromosomes in metaphase spread. Our studies reveal that most sex chromosomes (XY) remain on periphery and semi-periphery, 84.16% for X and 86.97% for Y respectively, in round metaphase spreads. The application of sex chromosome position in metaphase spreads is to easily find out sex chromosomes under microscope even without banding patterns. An another application is to identify or confirm s

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Hoang Thi Nhu, Phuong, Uyen Truong Thi Nha, Nhung Tran Thi, and Tung Hoang Thanh. "Metaphase chromosome spread preparation of citrus species." Academia Journal of Biology 47, no. 2 (2025): 107–16. https://doi.org/10.15625/2615-9023/22761.

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Citrus is one of the popular fruit crops in Vietnam with many commercial varieties, however, its cytogenetic characteristics are currently limited. This study was conducted to find out a procedure for metaphase chromosome preparation in some citrus species. The results showed that after a 4-hour treatment with 0.002 M 8-hydroxyquinoline solution, the mitose indices of the root tip samples differed by species. Specifically, the tangerine and orange samples yielded the highest results at 11:00 am, the lemon root sample at 10:00 am, and the kumquat sample at noon. The explants were fixed in carno

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Martínez, M., C. Cuadrado, and C. Romero. "Synaptic abnormalities in spread nuclei of Secale. I. Inbred lines." Genome 38, no. 4 (1995): 764–71. http://dx.doi.org/10.1139/g95-097.

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The meiotic behaviour at metaphase I of two inbred lines of Secale cereale showed a decrease in the frequency of bound arms and the presence of univalents. The study of synaptonemal complex formation revealed that these inbred lines had asynapsis and many nuclei did not complete the pairing process. Synaptic abnormalities such as fold-back loops, short pairing partner switches, interstitial nonhomologous pairing regions, and asynchrony between bivalents were associated and correlated with interlocking. It is suggested that the asynapsis is due to a failure in the interlocking repair. All these

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Martínez, M., C. Romero, and C. Cuadrado. "Synaptic abnormalities in spread nuclei of Secale. II. Secale vavilovii." Genome 38, no. 4 (1995): 772–79. http://dx.doi.org/10.1139/g95-098.

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Secale vavilovii PMCs have more univalents and a lower frequency of bound arms at metaphase I than other diploid Secale species. The spreading technique applied at prophase I showed that the nuclei were able to complete synapsis at pachytene. However, 25% of the nuclei analyzed, which had more than 90% of their total length paired, showed two abnormalities: long fold-back loops, which were located mainly on the nucleolar organizer bivalent, and pairing-partner switches, probably involving all the chromosome complement. These synaptic abnormalities are unusual in diploid species and give rise t

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Albini, S. M., and T. Schwarzacher. "In situ localization of two repetitive DNA sequences to surface-spread pachytene chromosomes of rye." Genome 35, no. 4 (1992): 551–59. http://dx.doi.org/10.1139/g92-082.

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Surface-spread pollen mother cells at meiotic prophase from Secale cereale (rye) were used for fluorescent DNA:DNA in situ localization of two tandemly repeated DNA sequences: pTa71, a wheat rDNA clone, and pSc119.2, a cloned 120-bp repeat from rye heterochromatin. The fluorescent hybridization signal, consisting of many yellow-green dots, was closely associated with the bivalent axes, corresponding to the synaptonemal complex, and located in the surrounding chromatin. The rDNA signal was associated with one bivalent, the smallest of the seven, at a distance about 13% of the bivalent length fr

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Mullinger, A. M., and R. T. Johnson. "Disassembly of the mammalian metaphase chromosome into its subunits: studies with ultraviolet light and repair synthesis inhibitors." Journal of Cell Science 87, no. 1 (1987): 55–69. http://dx.doi.org/10.1242/jcs.87.1.55.

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Metaphase chromosomes of a simian virus-transformed Indian muntjac cell line have been examined by scanning electron microscopy of material in which the fully packed metaphase structure is progressively relaxed. Such chromosomes are seen in standard, spread preparations of ultraviolet light-irradiated, metaphase-arrested cells, which have been incubated in the presence of inhibitors of DNA synthesis; they are processed for electron microscopy by trypsinization, further fixation and osmium impregnation. Decondensation is initially associated with a gradual elongation and loosening of the chromo

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Victor, Ekundare Olugbemi, Fagbuaro Omotayo, and Adeniran Idowu Isaac. "Preliminary cytogenetic study of <em style="mso-bidi-font-style: normal;">Ctenopoma kingsleyae</em> from Esa-Odo water reservoir, Osun State, Nigeria." Journal of Science of the University of Kelaniya 17, no. 1 (2024): 9–13. http://dx.doi.org/10.4038/josuk.v17i1.8100.

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Ctenopoma kingsleyae belongs to the family Anabantidae covers a wide geographic region. It has been morphologically described by a number of authors, yet it is lacking in adequate cytogenetic information, which is a setback to understanding its karyotypic evolution. This study, therefore, describes the karyotype of C. kingsleyae (n = 10) from Esa-Odo, Reservoir, Osun State, Nigeria. Metaphase chromosome spread was obtained from the gills of fish after intraperitoneal injection with 0.05% colchicines. Slides prepared were stained with Giemsa stain, images of metaphase spread were taken digitall

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Ma, Yan, M. Nurul Islam-Faridi, Charles F. Crane, David M. Stelly, H. James Price, and David H. Byrne. "A New Procedure to Prepare Slides of Metaphase Chromosomes of Roses." HortScience 31, no. 5 (1996): 855–57. http://dx.doi.org/10.21273/hortsci.31.5.855.

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To our knowledge, there has been no published technique to produce consistently high-quality slides of somatic chromosomes of roses (Rosa sp.). Therefore, various pretreatments, fixatives, digestions, stains, and maceration and squashing methods were tested to identify a procedure to produce clear, well-spread chromosomes from shoot tips. The best results were obtained after pretreatment in a mixture of 0.1% colchicine and 0.001 m 8-hydroxyquinoline for 4 h, and fixation in 2 acetone: 1 acetic acid (v/v) with 2% (w/v) polyvinylpyrrolidone. The darkest-stained chromosomes were obtained with car

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Martínez, M., C. Cuadrado, J. Sybenga, and C. Romero. "Differences in the synaptic pattern in two autotetraploid cultivars of rye with different quadrivalent frequencies at metaphase I." Genome 42, no. 4 (1999): 662–67. http://dx.doi.org/10.1139/g99-009.

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Synaptic behaviour of the two tetraploids rye cultivars Gigantón (G) and Tetrapico (T) displaying significant differences in their quadrivalent frequencies at metaphase I was analyzed by electron microscopy in surface-spread prophase I nuclei. A different behaviour was observed between the two cultivars; the synaptonemal complex (SC) quadrivalents frequency being significantly higher in G than in T at prophase I. Moreover, the G SC quadrivalents had more synaptic partner exchanges (SPEs) and their location was more distal than the T SC quadrivalents. However, inverse findings were found at met

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Chatterjee, R., and G. Jenkins. "Meiotic chromosome interactions in inbred autotetraploid rye (Secale cereale)." Genome 36, no. 1 (1993): 131–38. http://dx.doi.org/10.1139/g93-016.

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Electron microscopy of whole-mount surface-spread synaptonemal complex complements and conventional light microscopy of chromosomes at first metaphase of meiosis were used to compare the relative frequencies of pairing configurations at the two stages in inbred autotetraploid rye (Secale cereale L.). Statistical tests showed significantly fewer multivalents at first metaphase than expectations based on random initiation of synapsis at each telomeric site within each group of four homologues. Direct observations of synaptic behaviour of chromosomes showed that this deviation is due primarily to

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Książki na temat "Metaphase spread"

Bloch, Benjamin. Infection of joints and bones. Edited by Patrick Davey and David Sprigings. Oxford University Press, 2018. http://dx.doi.org/10.1093/med/9780199568741.003.0271.

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Infection can arise at any time within the bone or soft tissues, and sepsis should always be a consideration in the assessment of an acutely swollen, painful joint. An acute infection is an orthopaedic emergency, and prompt assessment should always be sought. Infection can affect either joints (septic arthritis) or bones (osteomyelitis). Septic arthritis can affect either a native joint or a prosthetic one, and the presentation can be acute or chronic. Similarly, osteomyelitis can present acutely or chronically, and can be due to haematogenous spread (particularly affecting the long-bone metap

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Części książek na temat "Metaphase spread"

Li, Le, Trude Schwarzacher, Paulina Tomaszewska, et al. "Protocols for Chromosome Preparations: Molecular Cytogenetics and Studying Genome Organization in Coffee." In Mutation Breeding in Coffee with Special Reference to Leaf Rust. Springer Berlin Heidelberg, 2023. http://dx.doi.org/10.1007/978-3-662-67273-0_21.

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AbstractCytological preparations from cell nuclei are required to count the number of chromosomes (including determining ploidy or aneuploidy), to investigate their morphology and organization. The results are valuable for genetic and evolutionary studies, and in breeding programs to understand species relationships, polyploidy, and potential introgression of chromosomes in hybrids between different species. Preparation of good chromosome spreads with well-separated metaphase chromosomes is the foundation of cytogenetic research including chromosomal mapping based on FISH (fluorescence in situ

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Akeson, Ellen C., and Muriel T. Davisson. "Analysing mouse chromosomal rearrangements with G-banded chromosomes." In Mouse Genetics and Transgenics, edited by Ian J. Jackson. Oxford University PressOxford, 1999. http://dx.doi.org/10.1093/oso/9780199637096.003.0006.

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Abstract This section describes the preparation of metaphase chromosomes from mouse peripheral blood lymphocytes for chromosomal typing of living mice. Included is a list of mouse strains and their relative response to phyto-haemagglutinin (PHA), a list of the requirements necessary for the production of well-spread metaphase chromosomes with good morphology for banding, a Giemsa banding (G-band) method for these chromosomes, and their classification into a karyotype. The identification of mouse chromosomes is presented with a detailed process for determining aberrant chromosomes and describin

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WALD, NIEL, and KENDALL PRESTON. "AUTOMATIC SCREENING OF METAPHASE SPREADS FOR CHROMOSOME ANALYSIS." In Image Processing in Biological Science. University of California Press, 2024. http://dx.doi.org/10.2307/jj.13168005.6.

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Gibbons, B., and B. H. Czepulkowski. "Cytogenetics in acute lymphoblastic leukaemia." In Human Cytogenetics. Oxford University PressOxford, 1992. http://dx.doi.org/10.1093/oso/9780199632909.003.0003.

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Abstract In the past, cytogenetic analysis of acute lymphoblastic leukaemia (ALL) has been hampered by the difficulty of obtaining good quality chromosomes. Poor spreading, fuzzy chromosomes, and indistinct bands meant that only about 50% of analysed cases were classified as having an abnormal clone. In addition, a number of cases also had metaphase spreads which were classified as unanalysable, and consequently failed.

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Gosden, John R., Ernst J. M. Speel,, and Yoshiro Shibasaki. "Primed in situ DNA synthesis (PRINS)." In PCR3. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199636327.003.0006.

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Abstract Techniques for visualizing DNA sequences on chromosomes have come a long way since Pardue and Gall (1,2) and John et al. (3) first showed that repeated sequences could be visualized in nuclei or on chromosomes by isotopic in situ hybridization. In those first experiments, the probe (RNA or DNA) was made radioactive with 3H by growing cells in the presence of labelled precursors, purifying the nucleic acid, and hybridizing it in situ to cytological preparations. Later came the use of Escherichia coli RNA polymerase to make a complementary RNA from a known DNA template (4,5). Refinement

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Davies, Derek C., and Bryan D. Youngt. "Chromosome analysis and sorting by flow cytometry." In Flow Cytometry. Oxford University PressOxford, 2000. http://dx.doi.org/10.1093/oso/9780199638253.003.0012.

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Abstract Many types of cancer and genetic disease are characterized by chromosomal abnormalities. These can be detected by conventional cytogenetics, which involves photographing banded chromosomes on metaphase spreads. Although this is a widely used technique in haematology, oncology, and pre-natal diagnosis, it is a time-consuming process that relies heavily on the skill and experience of the cytogeneticist. In addition, in cancerous cells, the sometimes complex karyotypes encountered mean that such analysis is often extremely difficult. An alternative approach is to prepare a monodispersed

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