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Artykuły w czasopismach na temat „Microscopy”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Schatten, G., J. Pawley, and H. Ris. "Integrated microscopy resource for biomedical research at the university of wisconsin at madison." Proceedings, annual meeting, Electron Microscopy Society of America 45 (August 1987): 594–97. http://dx.doi.org/10.1017/s0424820100127451.

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The High Voltage Electron Microscopy Laboratory [HVEM] at the University of Wisconsin-Madison, a National Institutes of Health Biomedical Research Technology Resource, has recently been renamed the Integrated Microscopy Resource for Biomedical Research [IMR]. This change is designed to highlight both our increasing abilities to provide sophisticated microscopes for biomedical investigators, and the expansion of our mission beyond furnishing access to a million-volt transmission electron microscope. This abstract will describe the current status of the IMR, some preliminary results, our upcomin

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Chen, Xiaodong, Bin Zheng, and Hong Liu. "Optical and Digital Microscopic Imaging Techniques and Applications in Pathology." Analytical Cellular Pathology 34, no. 1-2 (2011): 5–18. http://dx.doi.org/10.1155/2011/150563.

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The conventional optical microscope has been the primary tool in assisting pathological examinations. The modern digital pathology combines the power of microscopy, electronic detection, and computerized analysis. It enables cellular-, molecular-, and genetic-imaging at high efficiency and accuracy to facilitate clinical screening and diagnosis. This paper first reviews the fundamental concepts of microscopic imaging and introduces the technical features and associated clinical applications of optical microscopes, electron microscopes, scanning tunnel microscopes, and fluorescence microscopes.

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J. H., Youngblom, Wilkinson J., and Youngblom J.J. "Telepresence Confocal Microscopy." Microscopy and Microanalysis 6, S2 (2000): 1164–65. http://dx.doi.org/10.1017/s1431927600038319.

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The advent of the Internet has allowed the development of remote access capabilities to a growing variety of microscopy systems. The Materials MicroCharacterization Collaboratory, for example, has developed an impressive facility that provides remote access to a number of highly sophisticated microscopy and microanalysis instruments. While certain types of microscopes, such as scanning electron microscopes, transmission electron microscopes, scanning probe microscopes, and others have already been established for telepresence microscopy, no one has yet reported on the development of similar ca

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Youngblom, J. H., J. Wilkinson, and J. J. Youngblom. "Telepresence Confocal Microscopy." Microscopy Today 8, no. 10 (2000): 20–21. http://dx.doi.org/10.1017/s1551929500054146.

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The advent of the Internet has allowed the development of remote access capabilities to a growing variety of microscopy systems. The Materials MicroCharacterization Collaboratory, for example, has developed an impressive facility that provides remote access to a number of highly sophisticated microscopy and microanalysis instruments, While certain types of microscopes, such as scanning electron microscopes, transmission electron microscopes, scanning probe microscopes, and others have already been established for telepresence microscopy, no one has yet reported on the development of similar ca

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Brooks, Donald A. "The College of Microscopy — Meeting Rapidly Growing Microscopy Demands." Microscopy Today 15, no. 4 (2007): 51. http://dx.doi.org/10.1017/s1551929500055735.

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The McCrone Group Inc. recently announced the completion of a 40,000 sq ft addition to house its new College of Microscopy. Since its founding in 1956, The McCrone Group has grown into a multi-faceted organization and now encompasses three main organizations, McCrone Associates - the analytical service and consulting firm; McCrone Microscopes & Accessories - the microscope and instrument sales group; and, the College of Microscopy - the microscopy learning center. The newly completed addition houses the first and only College of Microscopy and offers the largest array of basic and advanced

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Martone, Maryann E. "Bridging the Resolution Gap: Correlated 3D Light and Electron Microscopic Analysis of Large Biological Structures." Microscopy and Microanalysis 5, S2 (1999): 526–27. http://dx.doi.org/10.1017/s1431927600015956.

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One class of biological structures that has always presented special difficulties to scientists interested in quantitative analysis is comprised of extended structures that possess fine structural features. Examples of these structures include neuronal spiny dendrites and organelles such as the Golgi apparatus and endoplasmic reticulum. Such structures may extend 10's or even 100's of microns, a size range best visualized with the light microscope, yet possess fine structural detail on the order of nanometers that require the electron microscope to resolve. Quantitative information, such as su

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Graef, M. De, N. T. Nuhfer, and N. J. Cleary. "Implementation Of A Digital Microscopy Teaching Environment." Microscopy and Microanalysis 5, S2 (1999): 4–5. http://dx.doi.org/10.1017/s1431927600013349.

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The steady evolution of computer controlled electron microscopes is dramatically changing the way we teach microscopy. For today’s microscopy student, an electron microscope may be just another program on the desktop of whatever computer platform he or she uses. This is reflected in the use of the term Desktop Microscopy. The SEM in particular has become a mouse and keyboard controlled machine, and running the microscope is not very different from using a drawing program or a word processor. Transmission electron microscopes are headed in the same direction.While one can debate whether or not

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Youngblom, J. H., J. Wilkinson, and J. J. Youngblom. "Confocal Laser Scanning Microscopy By Remote Access." Microscopy Today 7, no. 7 (1999): 32–33. http://dx.doi.org/10.1017/s1551929500064798.

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In recent years there have been a growing number of facilities interested in developing remote access capabilities to a variety of microscopy systems. While certain types of microscopes, such as electron microscopes and scanning probe microscopes have been well established for telepresence microscopy, no one has yet reported on the development of similar capabilities for the confocal microscope.At California State University, home to the CSUPERB (California State University Program for Education and Research in Biotechnology) Confocal Microscope Core Facility, we have established a remote acce

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Möller, Lars, Gudrun Holland, and Michael Laue. "Diagnostic Electron Microscopy of Viruses With Low-voltage Electron Microscopes." Journal of Histochemistry & Cytochemistry 68, no. 6 (2020): 389–402. http://dx.doi.org/10.1369/0022155420929438.

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Diagnostic electron microscopy is a useful technique for the identification of viruses associated with human, animal, or plant diseases. The size of virus structures requires a high optical resolution (i.e., about 1 nm), which, for a long time, was only provided by transmission electron microscopes operated at 60 kV and above. During the last decade, low-voltage electron microscopy has been improved and potentially provides an alternative to the use of high-voltage electron microscopy for diagnostic electron microscopy of viruses. Therefore, we have compared the imaging capabilities of three l

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10

O'Keefe, Michael A., John H. Turner, John A. Musante, et al. "Laboratory Design for High-Performance Electron Microscopy." Microscopy Today 12, no. 3 (2004): 8–17. http://dx.doi.org/10.1017/s1551929500052093.

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Since publication of the classic text on the electron microscope laboratory by Anderson, the proliferation of microscopes with field emission guns, imaging filters and hardware spherical aberration correctors (giving higher spatial and energy resolution) has resulted in the need to construct special laboratories. As resolutions iinprovel transmission electron microscopes (TEMs) and scanning transmission electron microscopes (STEMs) become more sensitive to ambient conditions. State-of-the-art electron microscopes require state-of-the-art environments, and this means careful design and implemen

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11

Jester, J. V., H. D. Cavanagh, and M. A. Lemp. "In vivo confocal imaging of the eye using tandem scanning confocal microscopy (TSCM)." Proceedings, annual meeting, Electron Microscopy Society of America 46 (1988): 56–57. http://dx.doi.org/10.1017/s0424820100102365.

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New developments in optical microscopy involving confocal imaging are now becoming available which dramatically increase resolution, contrast and depth of focus by optically sectioning through structures. The transparency of the anterior ocular structures, cornea and lens, make microscopic visualization and optical sectioning of the living intact eye an interesting possibility. Of the confocal microscopes available, the Tandem Scanning Reflected Light Microscope (referred to here as the Tandem Scanning Confocal Microscope), developed by Professors Petran and Hadravsky at Charles University in

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12

Ross, Frances M. "Materials Science in the Electron Microscope." MRS Bulletin 19, no. 6 (1994): 17–21. http://dx.doi.org/10.1557/s0883769400036691.

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This issue of the MRS Bulletin aims to highlight the innovative and exciting materials science research now being done using in situ electron microscopy. Techniques which combine real-time image acquisition with high spatial resolution have contributed to our understanding of a remarkably diverse range of physical phenomena. The articles in this issue present recent advances in materials science which have been made using the techniques of transmission electron microscopy (TEM), including holography, scanning electron microscopy (SEM), low-energy electron microscopy (LEEM), and high-voltage el

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Madrid-Wolff, Jorge, and Manu Forero-Shelton. "Protocol for the Design and Assembly of a Light Sheet Light Field Microscope." Methods and Protocols 2, no. 3 (2019): 56. http://dx.doi.org/10.3390/mps2030056.

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Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of spatial resolution. Light sheet microscopy is also a recent method that reduces illumination intensity while increasing the signal-to-noise ratio with respect to confocal microscopes. While faster and gentler to samples than confocals for a similar resolution, light sheet microscopy is still slower than light field microscopy since it must col

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14

Yamanaka, Kazushi. "Ultrasonic Force Microscopy." MRS Bulletin 21, no. 10 (1996): 36–41. http://dx.doi.org/10.1557/s0883769400031626.

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As an imaging method of elastic properties and subsurface features on the microscopic scale, the scanning acoustic microscope (SAM) provides spatial resolution comparable or superior to that of optical microscopes. Nondestructive evaluation methods of defects and elastic properties on the microscopic scale were developed by using the SAM, and they have been widely applied to various fields in science and technology. One major problem in acoustic microscopy is resolution. The best resolution of SAM with water as the coupling fluid has been 240 nm at a frequency of 4.4 GHz. At a more conventiona

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15

Sharmin, Nazlee, Ava Chow, and Alice Dong. "A Comparison Between Virtual and Conventional Microscopes in Health Science Education." Canadian Journal of Learning and Technology 49, no. 2 (2023): 1–20. http://dx.doi.org/10.21432/cjlt28270.

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Virtual microscopes are computer or web-based programs that enable users to visualize digital slides and mimic the experience of using a real light microscope. Traditional light microscopes have always been an essential teaching tool in health science education to observe and learn cell and tissue structures. However, studies comparing virtual and real light microscopes in education reported learners’ satisfaction with virtual microscopes regarding their usability, image quality, efficiency, and availability. Although the use of virtual or web-based microscopy is increasing, there is no equiva

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16

Govil, Anurag, David M. Pallister, and Michael D. Morris. "Three-Dimensional Digital Confocal Raman Microscopy." Applied Spectroscopy 47, no. 1 (1993): 75–79. http://dx.doi.org/10.1366/0003702934048497.

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We describe an iterative image restoration technique which functions as digital confocal microscopy for Raman images. We deconvolute the lateral and axial components of the microscope point spread function from a series of optical sections, to generate a stack of well-resolved Raman images which describe the three-dimensional topology of a sample. The technique provides an alternative to confocal microscopy for three-dimensional microscopic Raman imaging.

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Bracker, CE, and P. K. Hansma. "Scanning tunneling microscopy and atomic force microscopy: New tools for biology." Proceedings, annual meeting, Electron Microscopy Society of America 47 (August 6, 1989): 778–79. http://dx.doi.org/10.1017/s0424820100155864.

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A new family of scanning probe microscopes has emerged that is opening new horizons for investigating the fine structure of matter. The earliest and best known of these instruments is the scanning tunneling microscope (STM). First published in 1982, the STM earned the 1986 Nobel Prize in Physics for two of its inventors, G. Binnig and H. Rohrer. They shared the prize with E. Ruska for his work that had led to the development of the transmission electron microscope half a century earlier. It seems appropriate that the award embodied this particular blend of the old and the new because it demons

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18

Daberkow, I., and M. Schierjott. "Possibilities And Examples For Remote Microscopy Including Digital Image Acquisition, Transfer, and Archiving." Microscopy and Microanalysis 4, S2 (1998): 2–3. http://dx.doi.org/10.1017/s1431927600020134.

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Recent developments promise the possibility to externally control every aspect of microscopes through a computer interface. In combination with high-resolution cameras and feedback to the microscope, this can be leveraged to create highly automatic routines, e.g., to remotely correct astigmatism. Together with the development of fast computer networks this creates a new branch of microscopy, the so-called “telemicroscopy”. The goal of telemicroscopy is the control of a microscope over a large distance including the transfer of images with an acceptable repetition rate. A big advantage for elec

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Hudson, J. S. "Correlative microscopy techniques for material science." Proceedings, annual meeting, Electron Microscopy Society of America 53 (August 13, 1995): 688–89. http://dx.doi.org/10.1017/s0424820100139810.

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The microscopy center at Clemson University recently invested funds to provide a computer network system that incorporates all of its microscopes. The facility connects SEM, TEM, STM/AFM, Auger Microprobe and the light microscope to Sun workstations equipped with chemical analysis and imaging programs. Images from the network system microscopes can be sent to any of the workstations. I should like to review a few applications of correlative microscopy techniques related to material science; this is a technology that allows the acquisition of multiple data from a given sample. Often a given tec

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Storey, Malcolm. "Mycological Microscopy – choosing a stereo microscope." Field Mycology 20, no. 2 (2019): 48–50. http://dx.doi.org/10.1016/j.fldmyc.2019.03.006.

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Marti, O., B. Drake, S. Gould, and P. K. Hansma. "Atomic force microscopy and scanning tunneling microscopy with a combination atomic force microscope/scanning tunneling microscope." Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films 6, no. 3 (1988): 2089–92. http://dx.doi.org/10.1116/1.575191.

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Davidson, Michael W. "50 Most Frequently Asked Questions About Optical Microscopy." Microscopy Today 8, no. 6 (2000): 12–19. http://dx.doi.org/10.1017/s1551929500052780.

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A significant percentage of technical experts who employ optical microscopes have had little or no formal training in optical microscope basics. Some, typically, were required to use microscopes during their technical education but, in general, microscope terminology and technology was a sideline to their major training. As a result, many useful basic microscope technical details were not learned because they were not necessary to accomplish what was needed in order to survive their major class work. At Florida State University, we try to make the [earning of microscope technology an inherent

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Xianjun Zhang. "Development and Application of Cryogenic Optical Microscopy in Photosynthesis." Acta Physica Sinica 73, no. 21 (2024): 0. http://dx.doi.org/10.7498/aps.73.20241072.

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Efficient photosynthesis reaction thanks to the flexible energy regulation of two important pigment-protein complexes photosystem II (PSII) and photosystem I (PSI). Cryogenic spectral microscopy provides information about the spatial distribution and physiological functional states of photosynthetic components in photosynthetic organisms. Under low temperatures, the uphill energy transfer between pigments is efficiently suppressed so that the temperature-dependent PSI can be well analyzed. Therefore, a cryogenic spectral microscope allows us to discuss the physiological events surrounding PSII

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Martone, Maryann E., Andrea Thor, Stephen J. Young, and Mark H. Ellisman. "Correlated 3D Light and Electron Microscopy of Large, Complex Structures: Analysis of Transverse Tubules in Heart Failure." Microscopy and Microanalysis 4, S2 (1998): 440–41. http://dx.doi.org/10.1017/s1431927600022327.

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Light microscopic imaging has experienced a renaissance in the past decade or so, as new techniques for high resolution 3D light microscopy have become readily available. Light microscopic (LM) analysis of cellular details is desirable in many cases because of the flexibility of staining protocols, the ease of specimen preparation and the relatively large sample size that can be obtained compared to electron microscopic (EM) analysis. Despite these advantages, many light microscopic investigations require additional analysis at the electron microscopic level to resolve fine structural features

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Lin, Siru, Zhihang Lv, and Xiwei Huang. "Cost-effective scanning digital microscopic system for wide field-of-view and high-resolution biomedical imaging." Journal of Physics: Conference Series 2809, no. 1 (2024): 012033. http://dx.doi.org/10.1088/1742-6596/2809/1/012033.

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Abstract Microscopes serve as indispensable tools for exploring the microcosm and conducting biological detection. However, traditional microscopes fail to balance the field of view and resolution, limiting wide-field imaging capabilities. Here, we propose a low-cost, wide-field, high-resolution digital microscopy imaging system, providing high-quality and versatile microscopy tools for budget-constrained laboratories. The design of this system is based on the low-cost mechanical scanning and positioning platform provided by a computer numerical control (CNC) router architecture. The use of a

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Vilà, Anna, Sergio Moreno, Joan Canals, and Angel Diéguez. "A Compact Raster Lensless Microscope Based on a Microdisplay." Sensors 21, no. 17 (2021): 5941. http://dx.doi.org/10.3390/s21175941.

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Lensless microscopy requires the simplest possible configuration, as it uses only a light source, the sample and an image sensor. The smallest practical microscope is demonstrated here. In contrast to standard lensless microscopy, the object is located near the lighting source. Raster optical microscopy is applied by using a single-pixel detector and a microdisplay. Maximum resolution relies on reduced LED size and the position of the sample respect the microdisplay. Contrarily to other sort of digital lensless holographic microscopes, light backpropagation is not required to reconstruct the i

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Yang, Thomas Zhirui, and Yumin Wu. "Seeing cells without a lens: Compact 3D digital lensless holographic microscopy for wide-field imaging." Theoretical and Natural Science 12, no. 1 (2023): 61–72. http://dx.doi.org/10.54254/2753-8818/12/20230434.

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Optical microscopy is an essential tool for biomedical discoveries and cell diagnosis at micro- to nano-scales. However, conventional microscopes rely on lenses to record 2-D images of samples, which limits in-depth inspection of large volumes of cells. This research project implements a novel 3-D lensless microscopic imaging system that achieves a wide field of view, high resolution, and an extremely compact, cost-effective design: the Digital Lensless Holographic Microscope (DLHM).A lensless holographic microscope is built with only a light source, a sample, and an imaging chip (with other n

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Zemke, Valentina, Volker Haag, and Gerald Koch. "Wood identification of charcoal with 3D-reflected light microscopy." IAWA Journal 41, no. 4 (2020): 478–89. http://dx.doi.org/10.1163/22941932-bja10033.

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Abstract The present study focusses on the application of 3D-reflected light microscopy (3D-RLM) for the wood anatomical identification of charcoal specimens produced from domestic and tropical timbers. This special microscopic technique offers a detailed investigation of anatomical features in charcoal directly compared with the quality of field emission scanning electron microscopy (FESEM). The advantages of using the 3D-RLM technology are that fresh fracture planes of charcoal can be directly observed under the microscope without further preparation or surface treatment. Furthermore, the 3D

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Sun, Jiatong. "Super-resolution Microscopy and Photo-lithography: How can one inspire the other." Applied and Computational Engineering 66, no. 1 (2024): 211–16. http://dx.doi.org/10.54254/2755-2721/66/20240955.

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The principles of microscopy and lithography projection techniques are similar. To improve the resolution of the microscope or further reduce the image projection, both techniques face similar limitations, diffraction limits. The diffraction limit is a limitation of physical optics. In the development process of microscopes, to improve optical microscopy technology, researchers have proposed the idea of reducing the wavelength of light sources and increasing the numerical aperture based on the principle of diffraction limit generation. Modern scientists have proposed techniques to break throug

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Nessler, Randy, Ryan Potter, Jodi Stahl, Katina Wilson, Thomas Moninger, and Kenneth Moore. "Formal and Informal Microscopy Education at the University of Iowa Central Microscopy Research Facility: Project Centered Training." Microscopy and Microanalysis 7, S2 (2001): 814–15. http://dx.doi.org/10.1017/s1431927600030142.

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The University of Iowa Central Microscopy Research Facility (CMRF) has been in existence for 27 years. Starting out as a transmission electron microscopy (TEM) research laboratory, the facility has offered formal college courses. These courses require that students identify a project to investigate during the semester. Theory from the formal lecture is reinforced by work performed in the laboratory session. From its modest beginnings, the CMRF has continually grown. Currently, the facility offers two Confocal microscopes, two Scanning Electron Microscopes, a Scanning Probe Microscope, Energy D

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Reffner, John A., and William T. Wihlborg. "FR-IR Molecular Microanalysis System." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 2 (1990): 270–71. http://dx.doi.org/10.1017/s0424820100134958.

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The IRμs™ is the first fully integrated system for Fourier transform infrared (FT-IR) microscopy. FT-IR microscopy combines light microscopy for morphological examination with infrared spectroscopy for chemical identification of microscopic samples or domains. Because the IRμs system is a new tool for molecular microanalysis, its optical, mechanical and system design are described to illustrate the state of development of molecular microanalysis. Applications of infrared microspectroscopy are reviewed by Messerschmidt and Harthcock.Infrared spectral analysis of microscopic samples is not a new

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Cunningham, Brian. "Microscopy in the Real World - Instrumentation Requirements." Microscopy and Microanalysis 7, S2 (2001): 524–25. http://dx.doi.org/10.1017/s1431927600028695.

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In the last two decades, microscopy, in particular transmission electron microscopy, has moved from the research environment into industry. As such, the user requirements of the microscopes have changed. Previously, users required the highest performance in all aspects of microscopy e.g. imaging, analytical capabilities, with little regard to other factors. Today, additional requirements are being placed on areas such as ease of use, reliability, high throughput, expanded sample requirements, and networking capabilities. However, the “high performance” aspects of the instrumentation are still

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Borg, Thomas K., James A. Stewart, and Michael A. Sutton. "Imaging the Cardiovascular System: Seeing Is Believing." Microscopy and Microanalysis 11, no. 3 (2005): 189–99. http://dx.doi.org/10.1017/s1431927605050439.

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From the basic light microscope through high-end imaging systems such as multiphoton confocal microscopy and electron microscopes, microscopy has been and will continue to be an essential tool in developing an understanding of cardiovascular development, function, and disease. In this review we briefly touch on a number of studies that illustrate the importance of these forms of microscopy in studying cardiovascular biology. We also briefly review a number of imaging modalities such as computed tomography, (CT) Magnetic resonance imaging (MRI), ultrasound, and positron emission tomography (PET

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Chen, C. Julian. "Microscopic view of scanning tunneling microscopy." Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films 9, no. 1 (1991): 44–50. http://dx.doi.org/10.1116/1.577128.

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Kondo, Y., K. Yagi, K. Kobayashi, H. Kobayashi, and Y. Yanaka. "Construction Of UHV-REM-PEEM for Surface Studies." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 1 (1990): 350–51. http://dx.doi.org/10.1017/s0424820100180501.

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Recent development of ultra-high vacuum electron microscopy (UHV-EM) is very rapid. This is due to the fact that it can be applied to variety of surface science fields.There are various types of surface imaging in UHV condition; low energy electron microscopy (LEEM) [1], transmission (TEM) and reflection electron microscopy (REM) [2] using conventional transmission electron microscopes (CTEM) (including scanning TEM and REM)), scanning electron microscopy, photoemission electron microscopy (PEEM) [3] and scanning tunneling microscopy (STM including related techniques such as scanning tunneling

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Doerr, A., S. Badger, P. Brown, and S. Sahu. "Montages Link Microscopic to Macroscopic Information in Concrete Analys." Microscopy and Microanalysis 4, S2 (1998): 266–67. http://dx.doi.org/10.1017/s1431927600021450.

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One of the limitations of microscopy is that only a relatively small area is viewed and that both microscopic and macroscopic information is needed to better understand a process or relationship. Microscopic structures that can only be seen at high magnification may appear insignificant at magnifications required to see macroscopic structures. Montages from the scanning electron microscope (SEM) and optical microscope allow large areas to be displayed at relatively high magnifications revealing both macroscopic and microscopic features.The use of automated digital microscopy and image software

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Mansfield, John F. "Digital imaging: When should one take the plunge?" Proceedings, annual meeting, Electron Microscopy Society of America 54 (August 11, 1996): 602–3. http://dx.doi.org/10.1017/s0424820100165471.

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The current imaging trend in optical microscopy, scanning electron microscopy (SEM) or transmission electron microscopy (TEM) is to record all data digitally. Most manufacturers currently market digital acquisition systems with their microscope packages. The advantages of digital acquisition include: almost instant viewing of the data as a high-quaity positive image (a major benefit when compared to TEM images recorded onto film, where one must wait until after the microscope session to develop the images); the ability to readily quantify features in the images and measure intensities; and ext

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Schäfer, Max B., Sophie Weiland, Kent W. Stewart, and Peter P. Pott. "Compact Microscope Module for High- Throughput Microscopy." Current Directions in Biomedical Engineering 6, no. 3 (2020): 530–33. http://dx.doi.org/10.1515/cdbme-2020-3136.

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AbstractMicroscopy is an essential tool in research and science. However, it is relatively resource consuming regarding cost, time of usage, and consumable supplies. Current low-cost approaches provide good imaging quality but struggle in terms of versatility or applicability to varying setups. In this paper, a Compact Microscope Module for versatile application in custom-made setups or research projects is presented. As a first application and proof of concept, the use of the module in a High-Throughput Microscope for screening of samples in microtiter plates is shown. The Compact Microscope

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Collins, Joel T., Joe Knapper, Julian Stirling, et al. "Robotic microscopy for everyone: the OpenFlexure microscope." Biomedical Optics Express 11, no. 5 (2020): 2447. http://dx.doi.org/10.1364/boe.385729.

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Mehta, PK, DH Campbell, and JS Galehouse. "Quantitative Clinker Microscopy with the Light Microscope." Cement, Concrete and Aggregates 13, no. 2 (1991): 94. http://dx.doi.org/10.1520/cca10123j.

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You, Sungyong, Jerry Chao, Edward A. K. Cohen, E. Sally Ward, and Raimund J. Ober. "Microscope calibration protocol for single-molecule microscopy." Optics Express 29, no. 1 (2020): 182. http://dx.doi.org/10.1364/oe.408361.

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Wilke, V. "Optical scanning microscopy-The laser scan microscope." Scanning 7, no. 2 (1985): 88–96. http://dx.doi.org/10.1002/sca.4950070204.

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BULAT, TANJA, OTILIJA KETA, LELA KORIĆANAC, et al. "Radiation dose determines the method for quantification of DNA double strand breaks." Anais da Academia Brasileira de Ciências 88, no. 1 (2016): 127–36. http://dx.doi.org/10.1590/0001-3765201620140553.

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ABSTRACT Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (γH2AX). Immunofluorescent staining visualizes formation of γH2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position and intensity of fluorescent signal in each single cell. In practice there are problems in quantification of γH2AX. This paper is based on two issues: the determination of which technique should be applied concerning the radiation dose, and how to

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Wait, Eric C., Michael A. Reiche, and Teng-Leong Chew. "Hypothesis-driven quantitative fluorescence microscopy – the importance of reverse-thinking in experimental design." Journal of Cell Science 133, no. 21 (2020): jcs250027. http://dx.doi.org/10.1242/jcs.250027.

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ABSTRACTOne of the challenges in modern fluorescence microscopy is to reconcile the conventional utilization of microscopes as exploratory instruments with their emerging and rapidly expanding role as a quantitative tools. The contribution of microscopy to observational biology will remain enormous owing to the improvements in acquisition speed, imaging depth, resolution and biocompatibility of modern imaging instruments. However, the use of fluorescence microscopy to facilitate the quantitative measurements necessary to challenge hypotheses is a relatively recent concept, made possible by adv

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WOOLARD, DWIGHT, PEIJI ZHAO, CHRISTOPHER RUTHERGLEN, et al. "NANOSCALE IMAGING TECHNOLOGY FOR THz-FREQUENCY TRANSMISSION MICROSCOPY." International Journal of High Speed Electronics and Systems 18, no. 01 (2008): 205–22. http://dx.doi.org/10.1142/s012915640800528x.

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A novel nanoscale-engineering methodology is presented that has potential for the first-time development of a microscope-system capable of collecting terahertz (THz) frequency spectroscopic signatures from microscopic biological (bio) structures. This unique THz transmission microscopy approach is motivated by prior studies on bio-materials and bio-agents (e.g., DNA, RNA and bacterial spores) that have produced spectral features within the THz frequency regime (i.e., ~ 300 GHz to 1000 GHz) that appear to be representative of the internal structure and characteristics of the constituent bio-mol

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Hamm, Peter, Janina Schulz, and Karl-Hans Englmeier. "CONTENT-BASED AUTOFOCUSING IN AUTOMATED MICROSCOPY." Image Analysis & Stereology 29, no. 3 (2010): 173. http://dx.doi.org/10.5566/ias.v29.p173-180.

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Autofocusing is the fundamental step when it comes to image acquisition and analysis with automated microscopy devices. Despite all efforts that have been put into developing a reliable autofocus system, recent methods still lack robustness towards different microscope modes and distracting artefacts. This paper presents a novel automated focusing approach that is generally applicable to different microscope modes (bright-field, phase contrast, Differential Interference Contrast (DIC) and fluorescence microscopy). The main innovation consists in a Content-based focus search that makes use of a

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Edbert, Daniel, Ni Made Mertaniasih, Pepy Dwi Endraswari, and Eko Budi Koendhori. "Light wave filtration by colored cellophane to optimize microscopic contrast ratio in pulmonary tuberculosis sputum observation." F1000Research 11 (March 1, 2022): 254. http://dx.doi.org/10.12688/f1000research.109146.1.

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Introduction: Indonesia has the second highest number of Tuberculosis (TB) in the world and tuberculosis still pose as a global health priority problem. For the diagnostics, microscopy is still one of important modalities in TB diagnosis especially in peripheral areas for screening, case-finding, and treatment evaluation. The use of microscopy is limited to operator visual burden and specimen load. This study aims to modify the use of the microscope by using everyday item to increase microscope operator workload. Method: This is an analytic study of the use of colored cellophane in microscopic

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Anisovich, A. G., M. I. Markevich, and A. N. Malyshko. "Some particularities of microscopic investigation of non-metallic objects." Litiyo i Metallurgiya (FOUNDRY PRODUCTION AND METALLURGY), no. 2 (June 9, 2020): 75–80. http://dx.doi.org/10.21122/1683-6065-2020-2-75-80.

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The article deals with the comparative application of optical and raster microscopy for non-metallic objects and non-conducting surfaces. It is noted that this issue is not covered much in the special literature. There are practically no publications that compare and describe photos of the structure of materials obtained using fundamentally different microscopes, in particular, metallographic and raster. The causes of image distortion in a raster electron microscope in the study of dielectrics are considered. Comparative images of the oxidized surface, fabrics and natural leather obtained usin

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Sun Mingli, 孙明丽, 李驰野 Li Chiye, 陈睿黾 Chen Ruimin та 施钧辉 Shi Junhui. "微观探索的新光芒：便携式光声显微成像技术（特邀）". Laser & Optoelectronics Progress 61, № 6 (2024): 0618017. http://dx.doi.org/10.3788/lop232623.

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Clarke, Theodore M. "Effects of Abbe Condenser Spherical Aberration on Image Quality." Microscopy Today 13, no. 2 (2005): 20–25. http://dx.doi.org/10.1017/s1551929500051427.

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An earlier article in Microscopy Today, "Rediscovery of Darkfield Dispersion Staining while Building a Universal Student Microscope," noted that the darkfield inserts were made for Peter Cooke (MICA, Chicago, IL) to be used to teach high resolution dispersion staining in his advanced microscopy classes using Olympus BH2 microscopes and the Olympus 1.25 NA Abbe condenser. Peter has asked whether there is a better condenser design that will reduce the time and effort needed to switch between brightfield and darkfield.

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