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1

Benchimol-Reis, Luciana L. "Molecular Markers in Plant Breeding." Journal of Agricultural Science 15, no. 3 (2023): 58. http://dx.doi.org/10.5539/jas.v15n3p58.

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Molecular markers are an important tool for plant breeding. Since the 1980s, in response to the technology development, molecular marker approaches have been further diversified. The establishment of new-generation sequencing and high-throughput plant phenotyping has greatly decreased the time to genotype large numbers of individuals. For breeders who are not very familiar with molecular techniques and want to catch up with the advances in the field, this review offers basic knowledge. Each molecular marker technology has specific advantages as well as limitations. Molecular marker types, dive
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Lee, Tong Geon, Reza Shekasteband, Naama Menda, Lukas A. Mueller, and Samuel F. Hutton. "Molecular Markers to Select for the j-2–mediated Jointless Pedicel in Tomato." HortScience 53, no. 2 (2018): 153–58. http://dx.doi.org/10.21273/hortsci12628-17.

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The jointless pedicel trait of tomato conferred by the j-2 gene is widely used in processing markets for stem-free removal of fruit to accommodate mechanized harvest. Although current utilization of j-2 for fresh-market tomato breeding is limited, interest in this trait may increase as breeders seek to address high labor costs through the development of mechanically harvestable cultivars for the fresh market. Yet, the introduction of this trait into new market classes heavily relies on phenotypic selection because there are presently no high-throughput methods available to genotype j-2. Reliab
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Stalker, H. T., and L. G. Mozingo. "Molecular Markers of Arachis and Marker-Assisted Selection." Peanut Science 28, no. 2 (2001): 117–23. http://dx.doi.org/10.3146/i0095-3679-28-2-13.

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Abstract Many agronomic traits are difficult to select in Arachis hypogaea L. by conventional selection techniques, and marker-assisted selection offers an additional tool for obtaining improved germplasm lines. Molecular markers allow more efficient selection and offer a mechanism to eliminate undesirable traits associated with hybridizing diverse genotypes. The cultivated peanut has been analyzed by several marker systems, including RFLPs, RAPDs, AFLPs, and SSRs. Variation has been observed among diverse genotypes in approximately 5% of the markers analyzed, but the number is much lower betw
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Chiu, Sung Kay, Ming Hua Hsieh, and Chi Meng Tzeng. "Unique marker finder algorithm generates molecular diagnostic markers." International Journal of Bioinformatics Research and Applications 7, no. 1 (2011): 24. http://dx.doi.org/10.1504/ijbra.2011.039168.

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Soriano, Jose Miguel. "Molecular Marker Technology for Crop Improvement." Agronomy 10, no. 10 (2020): 1462. http://dx.doi.org/10.3390/agronomy10101462.

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Since the 1980s, agriculture and plant breeding have changed with the development of molecular marker technology. In recent decades, different types of molecular markers have been used for different purposes: mapping, marker-assisted selection, characterization of genetic resources, etc. These have produced effective genotyping, but the results have been costly and time-consuming, due to the small number of markers that could be tested simultaneously. Recent advances in molecular marker technologies such as the development of high-throughput genotyping platforms, genotyping by sequencing, and
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Salava, J., Y. Wang, B. Krška, et al. "Molecular genetic mapping in apricot." Czech Journal of Genetics and Plant Breeding 38, No. 2 (2012): 65–68. http://dx.doi.org/10.17221/6113-cjgpb.

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A genetic linkage map for apricot (Prunus armeniaca L.) has been constructed using amplified fragment length polymorphism (AFLP) markers in 80 BC1 individuals derived from a cross LE-3246 × Vestar. From 26 different primer combinations, a total of 248 AFLP markers were scored, of which, 40 were assigned to 8 linkage groups covering 315.8 cM of the apricot nuclear genome. The average interval between these markers was 7.7 cM. One gene (PPVres1) involved in resistance to PPV (Plum pox virus) was mapped. Two AFLP markers (EAA/MCAG8 and EAG/MCAT14) were found to be closely assoc
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Teneva, A., and M. P. Petrovic. "Application of molecular markers in livestock improvement." Biotehnologija u stocarstvu 26, no. 3-4 (2010): 135–54. http://dx.doi.org/10.2298/bah1004135t.

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With recent developments in DNA technologies, a large number of genetic polymorphisms at DNA sequence level has been introduced over the last decades as named DNA-based markers. The discovery of new class of DNA profiling markers has facilitated the development of marker-based gene tags, mapbased cloning of livestock important genes, variability studies, phylogenetic analysis, synteny mapping, marker-assisted selection of favourable genotypes, etc. The most commonly used DNA-based markers have advantages over the traditional phenotypic and biochemical markers since they provide data that can b
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Surma, Marian. "Molecular Marker of Tumours." Journal of Cancer Therapy 07, no. 10 (2016): 675–79. http://dx.doi.org/10.4236/jct.2016.710070.

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Koepke, John A. "Molecular marker test standardization." Cancer 69, S6 (1992): 1578–81. http://dx.doi.org/10.1002/1097-0142(19920315)69:6+<1578::aid-cncr2820691312>3.0.co;2-k.

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Khatoon, Arifa, Sumeet Verma, Gayatri Wadiye, and Anuprita Zore. "Molecular markers and their potentials." International Journal of Bioassays 5, no. 01 (2016): 4706. http://dx.doi.org/10.21746/ijbio.2016.01.003.

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The use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in plant molecular biotechnology and their genetic studies. There are three different types of markers viz. morphological, biochemical and DNA based molecular markers. These DNA based markers are differentiating in two types 1. Non PCR based (RFLP) and 2. PCR based markers (RAPD, AFLP, SSR, SNP etc.). Amongst others, the microsatellite DNA marker is one of the most widely used marker due to its easy use by simple PCR, followed by a denaturing gel electrophoresis. SNP (Single Nucleotide Po
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Tharun, Pantla Sai, Nilesh Talekar, and Vineeth Reddy Akkati. "Applications of Molecular Marker Implementation for Enhanced Oilseed Breeding through Marker-Assisted Selection (Mas)." Journal of Experimental Agriculture International 46, no. 5 (2024): 865–76. http://dx.doi.org/10.9734/jeai/2024/v46i52441.

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Oilseed crops are fundamental for various industrial and food purposes, necessitating advancements in breeding strategies to meet growing demands. Molecular markers and Marker-Assisted Selection (MAS) have emerged as powerful tools in oilseed crop improvement. This review explores the diverse applications of molecular markers and MAS in oilseed crops, highlighting their significance in enhancing breeding efficiency and genetic gains. The primary aim of this review is to provide a comprehensive overview of the application of molecular markers and MAS in oilseed crop breeding programs. Specifica
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Chesnokov, Yu V. "GENETIC MARKERS: COMPARATIVE CLASSIFICATION OF MOLECULAR MARKERS." Vegetable crops of Russia, no. 3 (July 25, 2018): 11–15. http://dx.doi.org/10.18619/2072-9146-2018-3-11-15.

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With the creation of the molecular markers allowing to carry out analysis of genotypes on the level initial genetic information – DNA, onset one of the most multifarious and one of the most large in number class of markers at the present day. It is concerned with that each separate nucleic acid sequence is unique on its structure. Set of molecular and genetic methods, named as DNA-fingerprinting, most wide used in modern investigations for solving different problems in different biological areas. In this connection, necessity in comparative classification of modern molecular and genetic marker
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Cherkasova, Olga, Yan Peng, Maria Konnikova, et al. "Diagnosis of Glioma Molecular Markers by Terahertz Technologies." Photonics 8, no. 1 (2021): 22. http://dx.doi.org/10.3390/photonics8010022.

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This review considers glioma molecular markers in brain tissues and body fluids, shows the pathways of their formation, and describes traditional methods of analysis. The most important optical properties of glioma markers in the terahertz (THz) frequency range are also presented. New metamaterial-based technologies for molecular marker detection at THz frequencies are discussed. A variety of machine learning methods, which allow the marker detection sensitivity and differentiation of healthy and tumor tissues to be improved with the aid of THz tools, are considered. The actual results on the
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Chikkaswamy, B. K., and Rabin Chandra Paramanik. "Molecular Distinction of Algae using Molecular Marker." International Journal of Current Microbiology and Applied Sciences 5, no. 9 (2016): 489–95. http://dx.doi.org/10.20546/ijcmas.2016.509.054.

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Surduse, Bhavana, P. A. Mohanapure, V. C. Khelurkar, et al. "Molecular Characterization of chickpea genotypes and Identification of true hybrids by molecular markers." International Journal of Agricultural and Applied Sciences 2, no. 1 (2021): 41–49. http://dx.doi.org/10.52804/ijaas2021.214.

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Chickpea (Cicer arietinum L.) is the third most important grain legume cultivated in the arid and semi-arid regions of the world. In the present study Six crossing combinations were executed in chickpea comprising Chanoli and PKV Kabuli 4 as female parents and Virat, BDNGK-798 and WR- 315 as resistant male parents. Total 54 markers including 13 SCoT, 31 SSR, 5 STMS, 3 RAPD, 1 SCAR, and 1 ISSR, used for parental polymorphism and polymorphic markers UBC-855, 66 % for TA-59 and 100 % for TA-110, TA-135 and GA-16 were further used to hybridity assessments of F1 plants. The PIC value for polymorphi
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16

Dey, T., and P. D. Ghosh. "Application of molecular markers in plant genome study." NBU Journal of Plant Sciences 4, no. 1 (2010): 1–9. http://dx.doi.org/10.55734/nbujps.2010.v04i01.001.

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The development of molecular techniques for genetic analysis has led to a great increase in our knowledge of plant genetics and our understanding of the structure and behaviour of plant genome. During last three decades, several powerful DNA based marker technologies have been developed for the assessment of genetic diversities and molecular marker assisted breeding technology. In plant systems, the prospects of DNA profiling and fingerprinting is becoming indispensable in the context of establishment of molecular phylogeny, assessment of somaclonal variants, characterization of plant genomics
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Dey, T., and P. D. Ghosh. "Application of molecular markers in plant genome study." NBU Journal of Plant Sciences 4, no. 1 (2010): 1–9. http://dx.doi.org/10.55734/nbujps.2010.v04i01.001.

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The development of molecular techniques for genetic analysis has led to a great increase in our knowledge of plant genetics and our understanding of the structure and behaviour of plant genome. During last three decades, several powerful DNA based marker technologies have been developed for the assessment of genetic diversities and molecular marker assisted breeding technology. In plant systems, the prospects of DNA profiling and fingerprinting is becoming indispensable in the context of establishment of molecular phylogeny, assessment of somaclonal variants, characterization of plant genomics
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18

Mcharo, M., D. LaBonte, R. O. M. Mwanga, and A. Kriegner. "Associating Molecular Markers with Virus Resistance to Classify Sweetpotato Genotypes." Journal of the American Society for Horticultural Science 130, no. 3 (2005): 355–59. http://dx.doi.org/10.21273/jashs.130.3.355.

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Molecular markers linked to resistance to sweetpotato chlorotic stunt closterovirus [SPCSV (genus Crinivirus, family Closteroviridae)] and sweetpotato feathery mottle virus [SPFMV (genus Potyvirus, family Potyviridae)] were selected using quantitative trait loci (QTL) analysis, discriminant analysis and logistic regression. Eighty-seven F1 sweetpotato [Ipomoea batatas (L.) Lam.] genotypes from a cross of `Tanzania' and `Wagabolige' landraces were used to generate DNA marker profiles for this study. Forty-five of the clones were resistant to SPCSV while 37 were resistant to SPFMV. A combination
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19

Zambelli, A. "THE IMPACT OF MOLECULAR GENETICS IN PLANT BREEDING: REALITIES AND PERSPECTIVES." Journal of Basic and Applied Genetics 30, no. 1 (2019): 11–15. http://dx.doi.org/10.35407/bag.2019.xxx.01.02.

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Even when conventional breeding was effective in achieving a continuous improvement in yield, Molecular Genetics tools applied in plant breeding contributed to maximize genetic gain. Thus, the use of DNA technology applied in agronomic improvement gave rise to Molecular Breeding, discipline which groups the different breeding strategies where genotypic selection, based on DNA markers, are used in combination with or in replacement of phenotypic selection. These strategies can be listed as: marker-assisted selection; marker-assisted backcrossing; marker assisted recurrent selection; and genomic
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S, Sahoo. "Molecular Marker Based Genetic Diversity Study of Curcuma Caesia Roxb. – A Mini Review." Open Access Journal of Microbiology & Biotechnology 8, no. 2 (2023): 1–4. http://dx.doi.org/10.23880/oajmb-16000266.

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The availability of genetic resource provides an opportunity for plant breeders to develop new cultivars with desirable characteristics and these characteristics encompass various traits preferred by farmers (high yield potential, larger seed size, etc) as well as traits favored by breeders (pests and disease resistance). Genetic resources are also essential for conservation, as they provide a basis for identifying threatened species and developing conservation strategies. Curcuma caesia Roxb. (Zingiberaceae) is an underutilized perennial herb with bluish-black tuberous rhizome having the char
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Rudchenko, Sergei, Steven Taylor, Nenad Milosavic, et al. "Amplification of Signal on Cell Surfaces in Molecular Cascades." Cells 12, no. 24 (2023): 2858. http://dx.doi.org/10.3390/cells12242858.

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We can formulate mixtures of oligonucleotide–antibody conjugates to act as molecular cascade-based automata that analyze pairs of cell surface markers (CD markers) on individual cells in a manner consistent with the implementation of Boolean logic—for example, by producing a fluorescent label only if two markers are present. While traditional methods to characterize cells are based on transducing signals from individual cell surface markers, these cascades can be used to combine into a single signal the presence of two or even more CDs. In our original design, oligonucleotide components irreve
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22

Li, X., L. Liu, Y. Gong, et al. "Molecular testing of cucumber hybrid genetic purity with RAPD marker." Seed Science and Technology 36, no. 2 (2008): 440–46. http://dx.doi.org/10.15258/sst.2008.36.2.17.

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Chen, F. Q., and M. R. Foolad. "A molecular linkage map of tomato based on a cross between Lycopersicon esculentum andL. pimpinellifolium and its comparison with other molecular maps of tomato." Genome 42, no. 1 (1999): 94–103. http://dx.doi.org/10.1139/g98-103.

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The wild species related to the cultivated tomato, Lycopersicon esculentum Mill., are a rich source of useful genes for germplasm improvement and varietal development. Characterization and utilization of exotic germplasm can be accelerated by the use of molecular linkage maps. Recently, we have identified an accession (LA722) within L. pimpinellifolium Jusl., a closely-related, red-fruited wild species of tomato, which exhibits a number of desirable agricultural characteristics including salt tolerance, disease resistance, and high fruit quality. The limited DNA marker polymorphism between L.
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Zhang, Mu-Qing, Xue-Fang Zheng, Ai-Li Yu, Jing-Sheng Xu, and Hui Zhou. "Molecular marker application in sugarcane." Sugar Tech 6, no. 4 (2004): 251–59. http://dx.doi.org/10.1007/bf02942505.

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Sakiyama, Ney Sussumu, Helaine Christine Cancela Ramos, Eveline Teixeira Caixeta, and Messias Gonzaga Pereira. "Plant breeding with marker-assisted selection in Brazil." Crop Breeding and Applied Biotechnology 14, no. 1 (2014): 54–60. http://dx.doi.org/10.1590/s1984-70332014000100009.

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Over the past three decades, molecular marker studies reached extraordinary advances, especially for sequencing and bioinformatics techniques. Marker-assisted selection became part of the breeding program routines of important seed companies, in order to accelerate and optimize the cultivar developing processes. Private seed companies increasingly use marker-assisted selection, especially for the species of great importance to the seed market, e.g. corn, soybean, cotton, and sunflower. In the Brazilian public institutions few breeding programs use it efficiently. The possible reasons are: lack
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Kutcher, H. R., K. L. Bailey, B. G. Rossnagel, and J. D. Franckowiak. "Linked morphological and molecular markers associated with common root rot reaction in barley." Canadian Journal of Plant Science 76, no. 4 (1996): 879–83. http://dx.doi.org/10.4141/cjps96-148.

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Selection of common root rot [Cochliobolus sativus (Ito &amp; Kurib.) Drechsl. ex Dastur] resistant barley germplasm is difficult due to the influence of environment on disease expression and the quantitative nature of resistance. Morphological and molecular markers associated with common root rot resistance could be used to increase the efficiency of selection of resistant germplasm and facilitate transfer to desirable barley genotypes Forty-five morphological marker sets consisting of four to six backcross-derived lines for each marker in a common background (cultivar Bowman) were planted in
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Chinnappareddy, L. R. D., K. Khandagale, A. Chennareddy, and V. G. Ramappa. "Molecular markers in the improvement of Allium crops." Czech Journal of Genetics and Plant Breeding 49, No. 4 (2013): 131–39. http://dx.doi.org/10.17221/111/2013-cjgpb.

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The genus Allium (Family: Alliaceae) is the most important among the bulbous vegetable crops. characterization of Alliums based on phenotypic traits is influenced by the environment and leads to biased diversity estimates. Recognizing the potential of DNA markers in plant breeding, researchers have adopted the molecular markers for marker-assisted selection (MAS), quantitative trait loci (QTL) mapping and characterization of different quality traits in Alliums. This review presents details about the use of DNA markers in Alliums for cultivar identification, diversity studies, SSR development,
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Blum, Eyal, Kede Liu, Michael Mazourek, Eun Young Yoo, Molly Jahn, and Ilan Paran. "Molecular mapping of the C locus for presence of pungency in Capsicum." Genome 45, no. 4 (2002): 702–5. http://dx.doi.org/10.1139/g02-031.

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Pungency owing to the presence of capsaicinoids is a unique character of pepper (Capsicum spp.). Capsaicinoids are produced in the placenta and it has long been known that a single dominant gene, C, is required for pungent genotypes to produce capsaicinoids. We mapped C to pepper chromosome 2 in a cross between a pungent Capsicum frutescens wild accession and a non-pungent Capsicum annuum bell pepper. This position confirmed results from earlier studies. The RFLP marker TG 205 cosegregated with C and two additional RFLP markers were also located within 1 cM. The recessive allele at the C locus
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Wang, Jian, Huijing Yan, Xiaozhen Jiao, et al. "Development of Specific Molecular and Phenotypic Marker-Based Haploid Inducers in Rice." Agronomy 13, no. 6 (2023): 1520. http://dx.doi.org/10.3390/agronomy13061520.

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Doubled haploid (DH) technology is an efficient strategy for producing completely homozygous lines for breeding programs. Mutations in the MATRILINEAL (MTL) phospholipase trigger intraspecific haploid induction in cereals. Although an in vivo haploid induction system based on OsMTL-edited plants has been established in rice (Oryza sativa), DH technology is still limited by other factors, such as haploid identification, which is one of the essential steps required for DH technology. In the study, we addressed this technical challenge by integrating specific molecular and phenotypic markers into
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Fang, D. Q., C. T. Federici, and M. L. Roose. "Development of molecular markers linked to a gene controlling fruit acidity in citrus." Genome 40, no. 6 (1997): 841–49. http://dx.doi.org/10.1139/g97-809.

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Fruit juice pH, titratable acidity, or citric acid content was measured in 6 populations derived from an acidless pummelo (pummelo 2240) (Citrus maxima (Burm.) Merrill). The acidless trait in pummelo 2240 is controlled by a single recessive gene called acitric. Using bulked segregant analysis, three RAPD markers were identified as linked to acitric. RAPD marker OpZ20410, which mapped 1.2 cM from acitric, was cloned and sequenced, and a sequence characterized amplified region (SCAR) marker (SCZ20) was developed. The SCZ20-410 marker allele that is linked to the acitric allele occurs only in pum
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Shamshin, I. N., M. V. Maslova, N. V. Drenova, M. L. Dubrovsky, and O. V. Parusova. "Assessment of fire blight resistance in apple clonal rootstocks using molecular markers." Proceedings on applied botany, genetics and breeding 181, no. 4 (2021): 185–91. http://dx.doi.org/10.30901/2227-8834-2020-4-185-191.

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Background. Clonal apple rootstocks are one of the main components of intensive gardening. The degree of rootstock damage by fire blight affects the resistance of the variety–rootstock combination. The paper presents a study on marking quantitative trait loci (QTL) of resistance to fire blight Erwinia amylovora in clonal apple rootstock.Materials and methods. A collection of 20 rootstock forms was analyzed. For the study, SCAR markers GE-8019, AE10-375 and microsatellite marker CH-F7-FB1 were used.Results. Polymorphism was observed for all three markers, and their various combinations in one g
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Yao, Chuxuan, Cuimian Zhang, Caili Bi, et al. "Establishment and Application of Multiplex PCR Systems Based on Molecular Markers for HMW-GSs in Wheat." Agriculture 12, no. 4 (2022): 556. http://dx.doi.org/10.3390/agriculture12040556.

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High-molecular-weight glutenin subunits (HMW-GSs) encoded by alleles at the Glu-A1, Glu-B1, and Glu-D1 loci confer unique end-use quality properties of common wheat (Triticum aestivum L.). Wheat accessions with the high-quality HMW-GSs combination of Ax2*/Bx7OE/Dx5 usually exhibit strong gluten characteristics. In order to stack these three high-quality subunit genes by molecular markers in strong gluten wheat breeding, an agarose gel-based multiplex PCR marker for these high-quality HMW-GSs and two agarose gel-based multiplex PCR markers detecting the homozygosity of Ax2* and Bx7OE subunits w
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Lu, Yun Hai, Geneviève Gagne, Bruno Grezes-Besset, and Philippe Blanchard. "Integration of a molecular linkage group containing the broomrape resistance gene Or5 into an RFLP map in sunflower." Genome 42, no. 3 (1999): 453–56. http://dx.doi.org/10.1139/g98-135.

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A linkage group containing the Or5 gene conferring resistance to Orobanche cumana race E, as well as 5 SCAR markers and 1 RAPD marker has been recently identified in sunflower. A SCAR marker RTS05, mapped 5.6 cM proximal to the Or5 locus, was analysed in an F2 population for which the segregation data of 80 RFLP markers (GIE cartisol - Phase II, France) were available. An association was found between the SCAR marker RTS05 and an RFLP marker S009 (32.1 cM, LOD = 4.7) that had been mapped to the linkage group 17 of the GIE Cartisol RFLP map. Another RFLP marker S010, tightly linked to S009 (0.0
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Sommarin, Mikael, Parashar Dhapola, Linda Geironson Ulfsson, et al. "Immunophenotypic- and Molecular Analysis of Human Hematopoietic Stem and Progenitor Heterogeneity." Blood 134, Supplement_1 (2019): 3701. http://dx.doi.org/10.1182/blood-2019-126407.

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Hematopoietic stem cells (HSCs) have the capacity to differentiate into all hematopoietic lineages and at the same time self-renew to maintain the HSC pool. HSCs have been thoroughly investigated using immunophenotypic-, molecular- and functional-analysis resulting in the development of protocols for high-purity prospective isolation of human HSCs. However, within the current state-of-the-art HSC populations, 90% of the cells lack stem cell activity, confounding molecular analysis of HSC function. Thus, identification of novel immunophenotypic markers to delineate the HSC population would impr
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Dolanská, L., and V. Čurn. "Identification of white clover (Trifolium repens L.) cultivars using molecular markers." Plant, Soil and Environment 50, No. 3 (2011): 95–100. http://dx.doi.org/10.17221/4013-pse.

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The different molecular analysis for specification of white clover (Trifolium repens L.) populations was studied between 2002 and 2003. RAPD, SSR (microsatellites), rDNA and PCR-RFLP markers were used for this study. The high genetic variation was detected among the cultivars but also within the cultivars by RAPD markers. For this reason RAPD markers were not found as a suitable marker system for determination of white clover cultivars. The distribution of low genetic variation of rDNA and PCR-RFLP markers was not able to differentiate cultivars. SSR and rDNA markers did not show variability o
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Ismail, Nor Asiah, M. Y. Rafii, T. M. M. Mahmud, M. M. Hanafi, and Gous Miah. "Genetic Diversity of Torch Ginger (Etlingera elatior) Germplasm Revealed by ISSR and SSR Markers." BioMed Research International 2019 (May 6, 2019): 1–14. http://dx.doi.org/10.1155/2019/5904804.

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Fifty-seven accessions of torch ginger (Etlingera elatior) collected from seven states in Peninsular Malaysia were evaluated for their molecular characteristics using ISSR and SSR markers to assess the pattern of genetic diversity and association among the characteristics. Diversity study through molecular characterization showed that high variability existed among the 57 torch ginger accessions. ISSR and SSR molecular markers revealed the presence of high genetic variability among the torch ginger accessions. The combination of different molecular markers offered reliable and convincing infor
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Gazal, Asima, Z. A. Dar, A. A. Lone, I. Abidi, and G. Ali. "Molecular breeding for resilience in maize - A review." Journal of Applied and Natural Science 7, no. 2 (2015): 1057–63. http://dx.doi.org/10.31018/jans.v7i2.731.

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Abiotic and biotic constraints have widespread yield reducing effects on maize and should receive high priority for maize breeding research. Molecular Breeding offers opportunities for plant breeders to develop cultivars with resilience to such diseases with precision and in less time duration. The term molecular breeding is used to describe several modern breeding strategies, including marker-assisted selection, marker-assisted backcrossing, marker-assisted recurrent selection and genomic selection. Recent advances in maize breeding research have made it possible to identify and map precisely
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Kim, Chang-Kug, Young-Joo Seol, Dong-Jun Lee, et al. "NABIC marker database: A molecular markers information network of agricultural crops." Bioinformation 9, no. 17 (2013): 887–88. http://dx.doi.org/10.6026/97320630009887.

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Jena, K. K., and D. J. Mackill. "Molecular Markers and Their Use in Marker-Assisted Selection in Rice." Crop Science 48, no. 4 (2008): 1266–76. http://dx.doi.org/10.2135/cropsci2008.02.0082.

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Foolad, Majid R. "Deployment of Molecular Markers and Marker-assisted Selection in Tomato Breeding." HortScience 40, no. 4 (2005): 1114A—1114. http://dx.doi.org/10.21273/hortsci.40.4.1114a.

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In tomato, Lycopersi conesculentum Mill., currently there are &gt;285 known morphological, physiological and disease resistance markers, 36 isozymes, and &gt;1000 RFLPs, which have been mapped onto the 12 tomato chromosomes. In addition, currently there are &gt;162,000 ESTs, of which ∼3.2% have been mapped. Several tomato genetic maps have been developed, mainly based on interspecific crosses between the cultivated tomato and its related wild species. The markers and maps have been used to locate and tag genes or QTLs for disease resistance and other horticultural characteristics. Such informa
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Jain, Ankit, Manish Roorkiwal, Sandip Kale, Vanika Garg, Ramakrishna Yadala, and Rajeev K. Varshney. "InDel markers: An extended marker resource for molecular breeding in chickpea." PLOS ONE 14, no. 3 (2019): e0213999. http://dx.doi.org/10.1371/journal.pone.0213999.

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An, Xia, Qin Liu, Jinyao Ying, et al. "Development of Expressed Sequence Tag–Simple Sequence Repeat Markers Related to the Salt-Stress Response of Kenaf (Hibiscus cannabinus)." Agronomy 13, no. 7 (2023): 1946. http://dx.doi.org/10.3390/agronomy13071946.

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Kenaf is one of the most important natural cannabis plants. Molecular marker-assisted breeding is vital for accelerating the breeding process of kenaf. However, the number of kenaf markers is insufficient for molecular marker-assisted breeding. Using transcriptome sequencing data for salt-stressed kenaf plants, the number and distribution of simple sequence repeats (SSRs) and single nucleotide variations (SNVs) in the expressed sequences were determined. The objectives of this study were to elucidate the sequence variations in kenaf genes expressed in response to salt stress and to identify st
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Munda, Mihael. "Molecular tissue markers integrated: A mathematical model approach to evaluating and predicting prostate cancer using molecular tissue markers." Acta Medico-Biotechnica 17, no. 2 (2024): 42–50. https://doi.org/10.18690/actabiomed.279.

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Purpose: The standard criteria available for the diagnosis of prostate cancer often do not sufficiently predict the course or outcome of this common disease. Evaluating molecular tissue markers, especially those that mark apoptosis and proliferation, in an integrated mathematical model, could contribute to a better understanding of the development and pathogenesis of this disease, aid in the selection of adequate therapies, and open new avenues of therapeutic research. Materials and methods: Paraffin-embedded prostatectomy specimens were stained according to an immunohistochemical protocol for
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Pradhan, D., and Usha Chakraborty. "Time-course accumulation of metabolites and expression of antioxidative enzymes in Glycine max under temperature stress." NBU Journal of Plant Sciences 4, no. 1 (2010): 25–30. http://dx.doi.org/10.55734/nbujps.2010.v04i01.004.

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The development of molecular techniques for genetic analysis has led to a great increase in our knowledge of plant genetics and our understanding of the structure and behaviour of plant genome. During last three decades, several powerful DNA based marker technologies have been developed for the assessment of genetic diversities and molecular marker assisted breeding technology. In plant systems, the prospects of DNA profiling and fingerprinting is becoming indispensable in the context of establishment of molecular phylogeny, assessment of somaclonal variants, characterization of plant genomics
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Pradhan, D., and Usha Chakraborty. "Time-course accumulation of metabolites and expression of antioxidative enzymes in Glycine max under temperature stress." NBU Journal of Plant Sciences 4, no. 1 (2010): 25–30. http://dx.doi.org/10.55734/nbujps.2010.v04i01.004.

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The development of molecular techniques for genetic analysis has led to a great increase in our knowledge of plant genetics and our understanding of the structure and behaviour of plant genome. During last three decades, several powerful DNA based marker technologies have been developed for the assessment of genetic diversities and molecular marker assisted breeding technology. In plant systems, the prospects of DNA profiling and fingerprinting is becoming indispensable in the context of establishment of molecular phylogeny, assessment of somaclonal variants, characterization of plant genomics
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Rout, G. R., S. K. Senapati, and S. Aparajita. "Study of relationships among twelve Phyllanthus species with the use of molecular markers." Czech Journal of Genetics and Plant Breeding 46, No. 3 (2010): 135–41. http://dx.doi.org/10.17221/74/2009-cjgpb.

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The present investigation was undertaken to describe the relationships among twelve species of Phyllanthus collected in India by help of molecular markers. In total, 259 marker loci were assessed, out of which 249 were polymorphic revealing 96.13% polymorphism. Nei's similarity index varied from 0.35 to 0.76 for RAPD (Random Amplified Polymorphic DNA) and from 0.31 to 0.76 for ISSR marker systems. Cluster analysis by the unweighted pair group method (UPGMA) of Dice coefficient of similarity generated dendrogram with more or less similar topology for both the analyses that offered a better expl
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Goswami, Manika, Kaushal Attri, and Isha Goswami. "Applications of Molecular Markers in Fruit Crops: A Review." International Journal of Economic Plants 9, no. 2 (2022): 121–26. http://dx.doi.org/10.23910/2/2022.0459.

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Markers are any trait of an organism that can be identified with confidence and relative ease, and can be followed in a mapping population or they can be defined as heritable entities associated with the economically important trait under the control of polygenes. Molecular markers have diverse applications in fruit crop improvement, particularly in the areas of genetic diversity and varietal identification studies, disease diagnostics, hybrid detection, sex differentiation and marker assisted selection. Molecular markers provide new directions to the efforts of plant breeders particularly in
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Jayesh, Vyas, Chopra Ashish, Kumar Vishnu, and Kumar Yadav Satendra. "Molecular markers and their applications in livestock improvement." Science World a monthly e magazine 2, no. 5 (2022): 480–85. https://doi.org/10.5281/zenodo.6534572.

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Molecular markers (also called DNA markers) are sites where differences in DNA sequences occur among members of the same species. Molecular markers have characteristic biological properties that can be detected and measured in parts of the body like the blood or tissue. A molecular marker is any kind of landmark along the DNA molecules of organisms
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Suprayogi, S., E. Oktaviani, A. Riyanto, B. Prakoso, R. Naufal, and N. A. Shafaa. "Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi." IOP Conference Series: Earth and Environmental Science 1297, no. 1 (2024): 012046. http://dx.doi.org/10.1088/1755-1315/1297/1/012046.

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Abstract The development of black rice with a fluffier texture at Universitas Jenderal Soedirman, which began in 2004, was carried out by crossing local black rice with the Mentik Wangi variety. At present, six black rice advanced breeding lines are ready for multi-location yield trials. Meanwhile, developing molecular marker-based plant breeding methods can facilitate a more efficient selection process. An important stage in developing molecular markers is marker validation, which aims to test the effectiveness in determining phenotypes in various genotypic and in new populations that differ
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Wang, Fei, James McD Stewart, and Jinfa Zhang. "Molecular markers linked to the Rf2 fertility restorer gene in cotton." Genome 50, no. 9 (2007): 818–24. http://dx.doi.org/10.1139/g07-061.

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Cytoplasmic male sterility (CMS) is a maternally inherited trait in which plants do not produce viable pollen. Fertility in plants with CMS can be recovered by nuclear restorer genes. Most restorer genes cloned so far are members of the pentatricopeptide repeat (PPR) protein family. The objective of our study was to use the CMS-D8 and restoration (Rf2) system of cotton ( Gossypium hirsutum L.) to develop more DNA markers for the Rf2 gene. In a backcross population with 112 plants, segregation of male fertility was 1 fertile : 1 sterile. Three new RAPD markers were identified for Rf2, one of wh
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