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Artykuły w czasopismach na temat "Monoclonal propagation"

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Park, Jinho, Kyoung Seong Choi, and J. Stephen Dumler. "Major Surface Protein 2 of Anaplasma phagocytophilum Facilitates Adherence to Granulocytes." Infection and Immunity 71, no. 7 (2003): 4018–25. http://dx.doi.org/10.1128/iai.71.7.4018-4025.2003.

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ABSTRACT Anaplasma phagocytophilum is an obligate intracellular bacterium that infects myeloid cells in the mammalian host. Msp2 (p44) is the major immunodominant outer-membrane protein of these bacteria. We hypothesized that Msp2 acts as an adhesin for A. phagocytophilum entry into granulocytes. This potential role was investigated by blocking binding with Msp2 monoclonal antibodies and by antagonizing binding and propagation with recombinant Msp2 (rMsp2) in vitro. With HL-60 cells, fresh human peripheral blood neutrophils, and a cell line devoid of the fucosylated platelet selectin glycoprot
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Akhatkulov, Bakhriddin Matlabovich. "SCIENTIFIC, METHODOLOGICAL AND THEORETICAL FOUNDATIONS OF MODERN BIOTECHNOLOGIES IN POTATO SEED PRODUCTION." Multidisciplinary Journal of Science and Technology 5, no. 5 (2025): 565–71. https://doi.org/10.5281/zenodo.15411255.

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<em>The article highlights the scientific, methodological, and theoretical foundations of using modern biotechnologies in potato seed production. Scientific approaches to the development of new potato varieties that are virus-free and adapted to climatic conditions using advanced biotechnological methods are studied. An analysis is conducted on genetic selection methods, molecular marking, plant tissue culture, monoclonal propagation, microbiological, ecological, and agronomic approaches, as well as "in vitro" propagation techniques and biological control measures.</em>
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Schluesener, H., C. Brunner, K. Vass, and H. Lassmann. "Therapy of rat autoimmune disease by a monoclonal antibody specific for T lymphoblasts." Journal of Immunology 137, no. 12 (1986): 3814–20. http://dx.doi.org/10.4049/jimmunol.137.12.3814.

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Abstract The central role of T lymphocytes in the initiation, regulation and propagation of autoimmune diseases defines them as most suitable targets for selective immunotherapy. The recent advance in culturing human and animal T cell lines allows us to select monoclonal antibodies specific for differentiation antigens expressed by activated T lymphocytes. We selected a monoclonal antibody cytotoxic for a subpopulation of activated rat T cells. In vivo, this antibody effectively blocks immune responses to foreign antigens or autoantigen and prevents development of autoimmune diseases like expe
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Norman, David J., and Anne M. Alvarez. "Monitoring the Spread of Xanthomonas Campestris pv. Dieffenbachiae Introduced from Symptomless Anthurium Cuttings into Production Fields." Journal of the American Society for Horticultural Science 121, no. 3 (1996): 582–85. http://dx.doi.org/10.21273/jashs.121.3.582.

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In field crops the origin and movement of bacterial inoculum is difficult to determine due to inadequate means of distinguishing strains of bacteria. In this study the introduction, establishment, and spread of Xanthomonas campestris pv. dieffenbachiae (McCulloch and Pirone) Dye into anthurium fields were examined by monitoring the distribution of serologically distinct strains recovered from propagation benches and production fields. One thousand Anthurium andraeanum Lind. plants were indexed for X. c. pv. dieffenbachiae and 962 were later introduced into a production field. Strains recovered
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Marquis, C. P., C. Harbour, J. P. Barford, and K. S. Low. "A comparison of different culture methods for hybridoma propagation and monoclonal antibody production." Cytotechnology 4, no. 1 (1990): 69–76. http://dx.doi.org/10.1007/bf00148812.

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Petyaev, Ivan M., Nayilia A. Zigangirova, Elena Y. Morgunova, Nigel H. Kyle, Elena D. Fedina, and Yuriy K. Bashmakov. "Resveratrol Inhibits Propagation ofChlamydia trachomatisin McCoy Cells." BioMed Research International 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/4064071.

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Resveratrol (RESV), an antifungal compound from grapes and other plants, has a distinct ability to inhibit theChlamydia (C.) trachomatisdevelopmental cycle in McCoy cells, a classic cell line used for chlamydial research. Inoculation ofC. trachomatiswith increasing amounts of RESV (from 12.5 to 100 μM) gave a dose-dependent reduction in the number of infected McCoy cells visualized by using monoclonal antibodies against chlamydial lipopolysaccharide. A similar trend has been observed with immunoassay for major outer membrane protein (MOMP). Furthermore, there was a step-wise reduction in the n
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Reuveny, S., D. Velez, L. Miller, and J. D. Macmillan. "Comparison of cell propagation methods for their effect on monoclonal antibody yield in fermentors." Journal of Immunological Methods 86, no. 1 (1986): 61–69. http://dx.doi.org/10.1016/0022-1759(86)90265-6.

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Sheoran, Abhineet S., Xiaochuan Feng, Inderpal Singh, et al. "Monoclonal Antibodies against Enterocytozoon bieneusi of Human Origin." Clinical Diagnostic Laboratory Immunology 12, no. 9 (2005): 1109–13. http://dx.doi.org/10.1128/cdli.12.9.1109-1113.2005.

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ABSTRACT Enterocytozoon bieneusi is clinically the most significant among the microsporidia infecting humans, causing chronic diarrhea, wasting, and cholangitis in individuals with human immunodeficiency virus/AIDS. The lack of immune reagents is largely due to the absence of methods for laboratory propagation of E. bieneusi. We recently described a procedure for the concentration and purification of spores from diarrheic stool of infected humans. Purified spores were used to immunize mice for production and screening of monoclonal antibodies (MAbs) against E. bieneusi. The eight immunoglobuli
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Bishop, A. L. "A Monoclonal Antibody Specific toAgrobacterium tumefaciensBiovar 3 and its Utilization for Indexing Grapevine Propagation Material." Phytopathology 79, no. 9 (1989): 995. http://dx.doi.org/10.1094/phyto-79-995.

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Wallisch, Michael, Yasser Khder, Monica T. Hinds, Erik I. Tucker, Dan Bloomfield, and Andras Gruber. "Abelacimab Reduces Thrombus Propagation in a Baboon Model of Vascular Graft Thrombosis." Blood 138, Supplement 1 (2021): 1027. http://dx.doi.org/10.1182/blood-2021-150535.

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Abstract Background: Factor XI (FXI) inhibition demonstrated strong efficacy in preventing thrombus formation in preclinical and clinical models of arterial and venous thrombosis. However, the effect of FXI inhibition in halting the progression of a formed clot remains largely unknown. Aims: This study aims to test whether abelacimab, a dual-acting FXI and activated FXI (FXIa) monoclonal antibody, is effective in halting clot formation and downstream growth when administered before or during active clot formation in an established baboon femoral arterio-venous (AV) shunt model. Methods: Three
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Rozprawy doktorskie na temat "Monoclonal propagation"

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Tsai, Yi-Ling, and 蔡易玲. "Propagation and characterization of anti thymidine glycol-BSA monoclonal antibodies." Thesis, 1997. http://ndltd.ncl.edu.tw/handle/77161847784567931093.

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Części książek na temat "Monoclonal propagation"

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Mithun, Sneha, Kinnari Chitnis, Bhakti Shetye, Mythili Kameswaran, Ashish Kumar Jha, and V. Rangarajan. "Antibody Based Radiopharmaceuticals in Clinical Development." In Targeted Radiopharmaceuticals and Imaging. Royal Society of Chemistry, 2025. https://doi.org/10.1039/9781837677139-00257.

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This chapter explores the utility and future prospects of antibody-based radiopharmaceuticals in clinical development. Different types of monoclonal antibodies have been used for diagnosis and treatment of various cancers by harnessing their inherent biological functions. Continuous advancements in processes, such as their production and fragmentation, have led to the development of newer and more effective targeting antibody molecules. Several tumor antigen targets, like epidermal growth factor receptor, carcinoembryonic antigen, programmed cell death protein 1, programmed cell death ligand 1, cluster of differentiation (CD) 5, CD 20, and CD 45 have been explored for development of radiopharmaceuticals for different tumor types. The high specificity and affinity to antigen targets, in addition to the emissions from radioisotopes, make radiolabeled monoclonal antibodies ideal agents for propagation of personalized medicine approaches. The aim of this chapter is to provide a comprehensive overview of the applications of radiolabeled monoclonal antibodies in oncological settings from bench to bedside.
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Pawelec, Graham, Fumiya Obata, David Sansom, et al. "Analysis of MHC class II-specific T cell clones." In MHC Volume 1. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199635542.003.0006.

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Abstract The application of T cell cloning in immunogenetics at the end of the 1970s (1) enhanced an early awareness of the complexity of functionally relevant lymphocyte stimulating determinants, mostly associated with MHC class II, and cytotoxic lymphocyte targets, mostly associated with MHC class I, and proved instrumental in establishing the mechanism of T cell recognition of antigen and alloantigen. Techniques for the generation, propagation and utilization of monoclonal strains of lymphocytes-T have become established as vital tools in diverse areas of immunology, but, with the exception of some early necessity-driven studies on the optimization of culture conditions (e.g. see ref. 2), there are relatively few published data on the practical details of establishing and maintaining human T cell clones (TCC). In fact, standard techniques work well enough, if not perfectly, that they have changed little since they were first established (1, 3). However, there seems to remain a widespread assumption, at least amongst those cellular and molecular immunologists with less personal experience in the area, that once T cell clones have been established, and provided that culture conditions are favourable, cultures can be maintained as permanent cell lines. While this may be true of a relatively small number of T cell clones, probably most experienced cloners will agree that, at least in humans, this is not the case for the majority of normal T cell lines (which should therefore be referred to as strains, not lines, according to tissue culturists’ standard nomenclature). Thus, the majority of T cells maintained in culture are found to have finite lifespans, and those examples of immortal lines in the literature may represent rare, not completely normal, variants.
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Jespers, Laurent, and Marc Fransen. "Interaction cloning using cDNA libraries displayed on phage." In Phage Display. Oxford University PressOxford, 2004. http://dx.doi.org/10.1093/oso/9780199638734.003.0012.

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Abstract Expression cloning of complementary DNA (cDNA) is an extremely valuable tool for the isolation and characterization of genes. Whereas nucleic acid probe-based screening relies on preliminary sequence information related to the gene of interest, screening of an expressed cDNA library only requires a natural ligand or a specific (monoclonal or polyclonal) antibody as probe (1, 2). In vitro screening of cDNA libraries expressed from A phage or plasmids involves affixing the translated products to a membrane prior to challenging with a labeled probe. This transfer can denature the cDNA-encoded proteins and may therefore com- promise detection. In contrast, the yeast-based two-hybrid system operates entirely in vivo by reconstituting a transcriptional activator to detect protein-- protein interactions via the expression of a reporter gene (3). Overall, these systems are not easily amenable to the screening of large libraries and this has triggered the development of several systems based on selection rather than screening. In view of sensitivity and selectivity, selection for a specific interaction through iterative enrichment steps is less demanding than screening. However, cDNA cloning via selection of expressed clones requires a physical linkage between each cDNA (for propagation) and its gene product (for detec- tion). One of the first selection systems designed for expression cloning of cDNA was developed for isolation of mammalian cell transformants expressing specific cell receptors (4). Recently, the advent of phage display technology (for reviews, see 5-6) as a powerful tool for selection of specific binding peptides or proteins from large libraries (210 clones) has provided the opportunity to develop this system for expression cloning of cDNA.
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Streszczenia konferencji na temat "Monoclonal propagation"

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Wernet, P., E. M. Scheider, P. Sarin, et al. "Demonstration of HIV-encoded Proteins in Cultured and in Uncultured CD 4 Positive Mononuclear Cells from Hemophilia Patients Employing Monoclonal Antibodies against p 15, p 24, GP 41, GP 120, and Reverse Transcriptase." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644683.

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In the light of the large percentage of hemophilia patients with antibodies to HIV the identification of a specific virus infection in comparison to HIV antibody negative hemophilia patients has reached crucial importance. The low success rates of direct virus culture techniques together with the as yet low AIDS-di-sease rate observed in these patients separate these patients from the other main risk groups. Within this context, we studied the expression of CD3, CD4, CD8, and HLA class II antigens on fixed cells after PHA stimulation and Interleukin 2 propagation as well as on untreated blood
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Raporty organizacyjne na temat "Monoclonal propagation"

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Antignus, Yehezkiel, Ernest Hiebert, Shlomo Cohen, and Susan Webb. Approaches for Studying the Interaction of Geminiviruses with Their Whitefly Vector Bemisia tabaci. United States Department of Agriculture, 1995. http://dx.doi.org/10.32747/1995.7604928.bard.

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The DNA of tomato yellow leaf curl virus (TYLCB) was detected in its whitefly vector, Bemisia tabaci, by dot spot hybridization as early as 1 h after acquisition access. The retention of the virus nucleic acid in the vector was at least 23 days after a 48 h acquisition access. However, the retention of TYLCV coat protein did not exceed 10 days. No replicative forms of TYLCV could be detected in B. tabaci, indicating a non-propagative relationship with the vector. Whiteflies were not able to accumulate naked virion ssDNA, virus cloned dsDNA, or virions with impaired coat protein. Deletion, fram
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