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Artykuły w czasopismach na temat „Multi-photon excitation microscopy”

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Data publikacji: 6 września 2023

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1

Piston, David W. "Multi-Photon Excitation Microscopy: An Old Idea in Quantum Theory Applied to Modern Scientific Problems." Microscopy and Microanalysis 6, S2 (2000): 1180–81. http://dx.doi.org/10.1017/s1431927600038393.

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Multi-photon excitation microscopy provides attractive advantages over confocal microscopy for three-dimensionalry resolved fluorescence imaging and photochemistry. The most commonly used type of multi-photon excitation is two-photon excitation where simultaneous absorption of two photons leads to a single quantitized event. The powerful advantages of using two-photon excitation microscopy arise from the basic physical principle that the absorption depends on the square of the excitation intensity. In practice, two-photon excitation is generated by focusing a single pulsed laser through the mi

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ROTHSTEIN, EMILY C., MICHAEL NAUMAN, SCOTT CHESNICK, and ROBERT S. BALABAN. "Multi-photon excitation microscopy in intact animals." Journal of Microscopy 222, no. 1 (2006): 58–64. http://dx.doi.org/10.1111/j.1365-2818.2006.01570.x.

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Masters, Barry R., and Peter T. C. So. "Multi-photon Excitation Microscopy and Confocal Microscopy Imaging of In Vivo Human Skin: A Comparison." Microscopy and Microanalysis 5, no. 4 (1999): 282–89. http://dx.doi.org/10.1017/s1431927699990311.

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Abstract: We compare here multi-photon excitation microscopy and tandem scanning reflected light confocal microscopy for the microscopic observation of human skin in vivo. Multi-photon excitation is induced by a 80-MHz pulse train of femtosecond laser pulses at 780 nm wavelength. This nonlinear microscopic technique is inherently suitable for tissue fluorescence imaging because of its deeper penetration depth and lower specimen photodamage. This technique has noninvasively obtained tissue structural information in human epidermis and dermis. Alternatively, tandem scanning confocal light micros

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Cheng, Ping-chin, Chi-Kuang Sun, Fu-Jen Kao, and Bai-Ling Lin. "Non-linear Spectral Microscopy-Multi-Photon Fl, SHG and THG." Microscopy and Microanalysis 7, S2 (2001): 1026–27. http://dx.doi.org/10.1017/s1431927600031202.

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The non-linear nature of multi-photon fluorescence (FL) excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry at high spatial resolution. Figure 1 shows a conventional optical micrograph of maize protoplasts, the time lapse fluorescence spectral change from a single chloroplast is shown in FIG 2. Under high intensity illumination, biological specimen not only emits fluorescence, but also generates harm

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Kao, F. J., B. L. Lin, and P. C. Cheng. "Multi-photon Fluorescence Micro-spectroscopy of Plant Tissues." Microscopy and Microanalysis 6, S2 (2000): 808–9. http://dx.doi.org/10.1017/s1431927600036539.

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Considering its non-linear nature, two-photon excitation may generate very different spectral response in samples when compared with single photon excitation. It is thus necessary to measure the two-photon spectra of samples, so that the two-photon fluorescence microscopic images can be properly interpreted. Fluorescence spectra obtained from bulk samples may not provide useful information for microscopy. For instance, due to the relatively small contribution to the total fluorescence intensity, a small number of fluorescent particles in a generally fluorescing specimen may escape detection wh

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6

Guo, Yong, Hongyi Han, Luwei Wang, et al. "Label free deep penetration single photon microscopic imaging with ultralong anti-diffracting beam." Applied Physics Letters 121, no. 2 (2022): 023701. http://dx.doi.org/10.1063/5.0097959.

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Label free single photon microscopic imaging has natural advantages in noninvasive in vivo tissue imaging such as high resolution and rapid imaging speed. Although label free multi-photon microscopy can be used for imaging thick tissue samples, it requires high excitation light power and is phototoxic to the samples. Conventional label free single photon microscopy requires lower excitation light power, but it has limited imaging depth. Observing some highly scattering thick tissue samples with single photon microscopy is a great challenge. To solve the problem, we developed a label free deep

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7

Cheng, P. C., B. L. Lin, F. J. Kao, C. K. Sun, and I. Johnson. "Multi-Photon Fluorescence Spectroum of Common Nucleic Acid Probes." Microscopy and Microanalysis 6, S2 (2000): 820–21. http://dx.doi.org/10.1017/s143192760003659x.

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Fluorescent probes are commonly used in biological fluorescence microscopy for tracking specific structures and sub-cellular compartments, and for indicating cellular ionic conditions. Recent development in multi-photon fluorescence microscopy has greatly expanded the usage of fluorescent probes in biomedical research. Considering its non-linear nature, two-photon excitation may generate very different fluorescence spectral response in the sample when compared with single photon excitation. It is thus necessary to measure the two-photon spectra of various fluorescent probes, so that two-photon

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8

Mertz, J. "Molecular photodynamics involved in multi-photon excitation fluorescence microscopy." European Physical Journal D - Atomic, Molecular and Optical Physics 3, no. 1 (1998): 53–66. http://dx.doi.org/10.1007/s100530050148.

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Schweitzer, Andreas, Heinz Eipel, and Christoph Cremer. "Rapid image acquisition in multi-photon excitation fluorescence microscopy." Optik 115, no. 3 (2004): 115–20. http://dx.doi.org/10.1078/0030-4026-00339.

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Goswami, Debabrata, Dhiman Das, and Soumendra Nath Bandyopadhyay. "Resolution enhancement through microscopic spatiotemporal control." Faraday Discussions 177 (2015): 203–12. http://dx.doi.org/10.1039/c4fd00177j.

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Operating at biologically benign conditions, multi-photon fluorescence imaging microscopy has benefitted immensely from recent developments in microscopic resolution enhancement. Fluorescence microscopy continues to be the best choice for experiments on live specimens, however, multi-photon fluorescence imaging often suffers from overlapping fluorescence of typical dyes used in microscopy, limiting its scope. This limitation has been the focus of our research where we show that by making simple modifications to the laser pulse structure, it is possible to resolve these overlapping fluorescence

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11

Masters, Barry R., and Peter T. C. So. "Confocal microscopy and multi-photon excitation microscopy of human skin in vivo." Optics Express 8, no. 1 (2001): 2. http://dx.doi.org/10.1364/oe.8.000002.

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Denk, Winfried. "Multi-Photon Microscopy, High Resolution Imaging Deep in Strongly Scattering Specimens." Microscopy and Microanalysis 3, S2 (1997): 301–2. http://dx.doi.org/10.1017/s1431927600008394.

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Imaging small structures substantially below the tissue surface in living specimens poses special challenges mainly because light is scattered by ever present refractive index inhomogeneities. Confocal microscoy removes the blurring caused by scattered and out-of-focus light but does so only at the expense of photodynamic damage that is often unacceptable when observing live specimens.Multi-photon absorption microscopy[l] solves these problems because excitation is virtually limited to the focal plane. Out-of-focus photobleaching and photodamage are therefore eliminated. In scattering samples

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13

Yan, Wei, Yangrui Huang, Luwei Wang, et al. "Multi-Color Two-Photon Microscopic Imaging Based on A Single-Wavelength Excitation." Biosensors 12, no. 5 (2022): 307. http://dx.doi.org/10.3390/bios12050307.

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Two-photon probes with broad absorption spectra are beneficial for multi-color two-photon microscopy imaging, which is one of the most powerful tools to study the dynamic processes of living cells. To achieve multi-color two-photon imaging, multiple lasers and detectors are usually required for excitation and signal collection, respectively. However, one makes the imaging system more complicated and costly. Here, we demonstrate a multi-color two-photon imaging method with a single-wavelength excitation by using a signal separation strategy. The method can effectively solve the problem of spect

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14

Malak, Henryk. "Up-Conversion and Two-Photon Excitation Fluorescence Properties of Phloxine B." Microscopy and Microanalysis 5, S2 (1999): 500–501. http://dx.doi.org/10.1017/s1431927600015828.

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A dye, Phloxine B, a common food coloring and one of the active components of a photoreactive insecticide was recently approved by Food and Drug Administration as D&C Red #28 for use in drugs and cosmetics. Phloxine B is also one of the most widely use stain in fluorescence microscopy. However, in spite of the widespread interest in multi-photon spectroscopy and imaging, no information is available on the electronic transitions properties of Phloxine B with red edge fluorescence excitation and with multi-photon excitation.In the present report we described the steady state and time-resolve

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15

Choe, Kibaek, Yusaku Hontani, Tianyu Wang, et al. "Intravital three-photon microscopy of entire mouse lymph node." Journal of Immunology 204, no. 1_Supplement (2020): 86.4. http://dx.doi.org/10.4049/jimmunol.204.supp.86.4.

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Abstract For the last 20 years, intravital confocal and two-photon microscopy have been powerful tools to explore dynamic immune cell behavior in mouse lymph node. However, they can only image ~100 and ~300 μm depth respectively while a peripheral lymph node of adult mouse has 600 – 1000 μm thickness. Here, we visualized whole thickness of adult mouse popliteal lymph node with intravital three-photon microscopy. Three-photon excitation significantly improved a signal-to-background ratio compared to two-photon excitation even at the same excitation wavelength. The capability to image the full d

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16

Buist, Muller, Squier, and Brakenhoff. "Real time two-photon absorption microscopy using multi point excitation." Journal of Microscopy 192, no. 2 (1998): 217–26. http://dx.doi.org/10.1046/j.1365-2818.1998.00431.x.

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Fisher, Wait G., and Eric A. Wachter. "Improved Signal Processing in Multi-Photon Imaging." Microscopy and Microanalysis 6, S2 (2000): 800–801. http://dx.doi.org/10.1017/s1431927600036497.

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Multi-photon excitation has been used in microscopy for nearly a decade, providing a number of demonstrated advantages over other methods for fluorescence imaging. Because excitation is achieved using longer, less energetic light, photodamage and photobleaching of the sample are reduced. Furthermore, since excitation occurs only at the focal point, this approach allows the practical collection of three-dimensionally resolved fluorescence images of live cells. However, due to the small two-photon cross-section of most fluorophores, pulsed lasers are required to generate detectable signal levels

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18

Chang, Ching-Wei, and Mary-Ann Mycek. "Precise fluorophore lifetime mapping in live-cell, multi-photon excitation microscopy." Optics Express 18, no. 8 (2010): 8688. http://dx.doi.org/10.1364/oe.18.008688.

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Masters, B. R., P. T. C. So, and E. Gratton. "Optical Biopsy of In Vivo Human Skin: Multi-photon Excitation Microscopy." Lasers in Medical Science 13, no. 3 (1998): 196–203. http://dx.doi.org/10.1007/s101030050074.

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Matsumoto, Naoya, Alu Konno, Takashi Inoue, Koyo Watanabe, and Shigetoshi Okazaki. "Amplitude-modulation-type multi-ring mask for two-photon excitation scanning microscopy." OSA Continuum 4, no. 6 (2021): 1696. http://dx.doi.org/10.1364/osac.419804.

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Vlieg, Redmar C., and John van Noort. "Multiplexed two-photon excitation spectroscopy of single gold nanorods." Journal of Chemical Physics 156, no. 9 (2022): 094201. http://dx.doi.org/10.1063/5.0073208.

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Plasmonic metallic nanoparticles are commonly used in (bio-)sensing applications because their localized surface plasmon resonance is highly sensitive to changes in the environment. Although optical detection of scattered light from single particles provides a straightforward means of detection, the two-photon luminescence (TPL) of single gold nanorods (GNRs) has the potential to increase the sensitivity due to the large anti-Stokes shift and the non-linear excitation mechanism. However, two-photon microscopy and spectroscopy are restricted in bandwidth and have been limited by the thermal sta

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22

NEMOTO, Tomomi, Ryosuke KAWAKAMI, and Terumasa HIBI. "Improvement in Tissue Penetration Depth and Spatial Resolution of Multi-Photon Laser Excitation Microscopy." Review of Laser Engineering 41, no. 2 (2013): 107. http://dx.doi.org/10.2184/lsj.41.2_107.

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Kantelhardt, Sven Rainer, Jan Leppert, Jan Werner Kantelhardt, Erich Reusche, Gereon Hüttmann, and Alf Giese. "Multi-photon excitation fluorescence microscopy of brain-tumour tissue and analysis of cell density." Acta Neurochirurgica 151, no. 3 (2009): 253–62. http://dx.doi.org/10.1007/s00701-009-0188-6.

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Dal Fovo, Alice, Mikel Sanz, Mohamed Oujja, et al. "In-Depth Analysis of Egg-Tempera Paint Layers by Multiphoton Excitation Fluorescence Microscopy." Sustainability 12, no. 9 (2020): 3831. http://dx.doi.org/10.3390/su12093831.

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The non-invasive depth-resolved imaging of pictorial layers in paintings by means of linear optical techniques represents a challenge in the field of Cultural Heritage (CH). The presence of opaque and/or highly-scattering materials may obstruct the penetration of the radiation probe, thus impeding the visualization of the stratigraphy of paintings. Nonlinear Optical Microscopy (NLOM), which makes use of tightly-focused femtosecond pulsed lasers as illumination sources, is an emerging technique for the analysis of painted objects enabling micrometric three-dimensional (3D) resolution with good

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25

George, Nicholas M., Arianna G. Polese, Greg Futia, et al. "2507 A novel multi-photon microscopy method for neuronavigation in deep brain stimulation surgery." Journal of Clinical and Translational Science 2, S1 (2018): 2–3. http://dx.doi.org/10.1017/cts.2018.40.

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OBJECTIVES/SPECIFIC AIMS: The goal for this project is to determine the feasibility of using a novel multi-photon fiber-coupled microscope to aid surgeons in localizing STN during surgeries. In order to accomplish this goal, we needed to identify the source of a strong autofluorescent signal in the STN and determine whether we could use image classification methods to automatically distinguish STN from surrounding brain regions. METHODS/STUDY POPULATION: We acquired 3 cadaveric brains from the University of Colorado Anschutz Medical Campus, Department of Pathology. Two of these brains were non

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26

Neu, Thomas R., Stefan Woelfl, and John R. Lawrence. "Three-dimensional differentiation of photo-autotrophic biofilm constituents by multi-channel laser scanning microscopy (single-photon and two-photon excitation)." Journal of Microbiological Methods 56, no. 2 (2004): 161–72. http://dx.doi.org/10.1016/j.mimet.2003.10.012.

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Robbins, Emma, Stéphanie Leroy-Lhez, Nicolas Villandier, Marek Samoć, and Katarzyna Matczyszyn. "Prospects for More Efficient Multi-Photon Absorption Photosensitizers Exhibiting Both Reactive Oxygen Species Generation and Luminescence." Molecules 26, no. 20 (2021): 6323. http://dx.doi.org/10.3390/molecules26206323.

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The use of two-photon absorption (TPA) for such applications as microscopy, imaging, and photodynamic therapy (PDT) offers several advantages over the usual one-photon excitation. This creates a need for photosensitizers that exhibit both strong two-photon absorption and the highly efficient generation of reactive oxygen species (ROS), as well as, ideally, bright luminescence. This review focuses on different strategies utilized to improve the TPA properties of various multi-photon absorbing species that have the required photophysical properties. Along with well-known families of photosensiti

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OTOMO, Kohei, Terumasa HIBI, Takashi MURATA, et al. "Multi-point Scanning Two-photon Excitation Microscopy by Utilizing a High-peak-power 1042-nm Laser." Analytical Sciences 31, no. 4 (2015): 307–13. http://dx.doi.org/10.2116/analsci.31.307.

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Gualda, Emilio J., George Filippidis, Kristalia Melessanaki, and Costas Fotakis. "Third-Harmonic Generation and Multi-Photon Excitation Fluorescence Imaging Microscopy Techniques for Online Art Conservation Diagnosis." Applied Spectroscopy 63, no. 3 (2009): 280–85. http://dx.doi.org/10.1366/000370209787598898.

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Faraldi, F., G. J. Tserevelakis, G. Filippidis, G. M. Ingo, C. Riccucci, and C. Fotakis. "Multi photon excitation fluorescence imaging microscopy for the precise characterization of corrosion layers in silver-based artifacts." Applied Physics A 111, no. 1 (2013): 177–81. http://dx.doi.org/10.1007/s00339-013-7548-z.

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Bickford, Lissett, Jiantang Sun, Kun Fu, et al. "Enhanced multi-spectral imaging of live breast cancer cells using immunotargeted gold nanoshells and two-photon excitation microscopy." Nanotechnology 19, no. 31 (2008): 315102. http://dx.doi.org/10.1088/0957-4484/19/31/315102.

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Liu, I.-Ting, Chia-Sheng Yen, Wen-Lung Wang, et al. "Predict Early Recurrence of Resectable Hepatocellular Carcinoma Using Multi-Dimensional Artificial Intelligence Analysis of Liver Fibrosis." Cancers 13, no. 21 (2021): 5323. http://dx.doi.org/10.3390/cancers13215323.

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Background: Liver fibrosis is thought to be associated with early recurrence of hepatocellular carcinoma (HCC) after resection. To recognize HCC patients with higher risk of early recurrence, we used a second harmonic generation and two-photon excitation fluorescence (SHG/TPEF) microscopy to create a fully quantitative fibrosis score which is able to predict early recurrence. Methods: The study included 81 HCC patients receiving curative intent hepatectomy. Detailed fibrotic features of resected hepatic tissues were obtained by SHG/TPEF microscopy, and we used multi-dimensional artificial inte

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Lavin, C. A., W. A. Mohler, H. H. Keating, and J. G. White. "Capturing Developmental Events of the C. Elegans Embryo by High Pressure Freezing After Monitoring by a Multi-Photon Imaging System." Microscopy and Microanalysis 3, S2 (1997): 291–92. http://dx.doi.org/10.1017/s1431927600008345.

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High pressure freezing enables the rapid arrest of developmental events without prefixation. Standard chemical fixation is a time dependent event and may cause artifacts in sensitive cytoskeletal components. We are studying two developmental events in embryonic Caenorhabditis elegans: that involve changes in the cytoskeleton: spindle alignment and membrane fusion. The mitotic spindle undergoes rapid rotational alignment prior to certain differentiative divisions. We are trying to capture these events by anticipation their timing and rapid freezing. Precursor hypodermal cells of embryonic C. el

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Nevin, A., D. Comelli, I. Osticioli, et al. "Multi-photon excitation fluorescence and third-harmonic generation microscopy measurements combined with confocal Raman microscopy for the analysis of layered samples of varnished oil films." Applied Physics A 100, no. 3 (2010): 599–606. http://dx.doi.org/10.1007/s00339-010-5644-x.

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Zandt, Bas-Jan, Are Losnegård, Erlend Hodneland, Margaret Lin Veruki, Arvid Lundervold, and Espen Hartveit. "Semi-automatic 3D morphological reconstruction of neurons with densely branching morphology: Application to retinal AII amacrine cells imaged with multi-photon excitation microscopy." Journal of Neuroscience Methods 279 (March 2017): 101–18. http://dx.doi.org/10.1016/j.jneumeth.2017.01.008.

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Zandt, Bas-Jan, Jian Hao Liu, Margaret Lin Veruki, and Espen Hartveit. "AII amacrine cells: quantitative reconstruction and morphometric analysis of electrophysiologically identified cells in live rat retinal slices imaged with multi-photon excitation microscopy." Brain Structure and Function 222, no. 1 (2016): 151–82. http://dx.doi.org/10.1007/s00429-016-1206-0.

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Brzoska, Tomasz, Tomasz W. Kaminski, Egemen Tutuncuoglu, Mark T. Gladwin, and Prithu Sundd. "Two Stage Intravital Imaging Mouse Model to Assess Venous Thromboembolism in Sickle Cell Disease." Blood 138, Supplement 1 (2021): 3225. http://dx.doi.org/10.1182/blood-2021-154317.

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Abstract Sickle cell disease (SCD) patients have an increased risk of venous thromboembolism (VTE). Population-based studies demonstrated that VTE has a cumulative incidence rate of approximately 25% in adult SCD patients and is associated with higher risk of mortality. VTE in SCD is most commonly manifested as deep vein thrombosis (DVT) with associated pulmonary embolism (PE). Although autopsy studies have regularly discovered pulmonary thromboembolic lesions in SCD patients, the pathophysiology of VTE in SCD remains largely unknown mostly due to the lack of relevant animal VTE model. Underst

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Song, Ying, Zongwei Xu, Tao Liu, et al. "Depth Profiling of Ion-Implanted 4H–SiC Using Confocal Raman Spectroscopy." Crystals 10, no. 2 (2020): 131. http://dx.doi.org/10.3390/cryst10020131.

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For silicon carbide (SiC) processed by ion-implantation, dedicated test structure fabrication or destructive sample processing on test wafers are usually required to obtain depth profiles of electrical characteristics such as carrier concentration. In this study, a rapid and non-destructive approach for depth profiling is presented that uses confocal Raman microscopy. As an example, a 4H–SiC substrate with an epitaxial layer of several micrometers thick and top layer in nanoscale that was modified by ion-implantation was characterized. From the Raman depth profiling, longitudinal optical (LO)

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Sundd, Prithu, Maritza Ann Jimenez, Margaret F. Bennewitz, et al. "Hairy Platelet-Derived Extracellular Vesicles Promote Lung Vaso-Occlusion in Sickle Cell Disease." Blood 130, Suppl_1 (2017): 958. http://dx.doi.org/10.1182/blood.v130.suppl_1.958.958.

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Abstract Background: Acute chest syndrome (ACS) is a type of acute lung injury and the leading cause of mortality in Sickle Cell Disease (SCD). Current treatments for ACS are primarily supportive, and there is a critical need for rescue therapies. ACS is often a sequela of acute systemic vaso-occlusive crisis and preceded by thrombocytopenia. However, the role of platelets in the pathogenesis of ACS remains largely unknown. Methods: We used our validated model of vaso-occlusive crisis in transgenic, humanized SCD mice, which is triggered by intravenous challenge with nanogram levels of the TLR

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Vats, Ravi, Egemen Tutuncuoglu, Jesus Tejero, Gray Shaw, and Prithu Sundd. "Tandem P-Selectin Glycoprotein Ligand Immunoglobulin Prevents Lung Vaso-Occlusion in SCD Mice." Blood 132, Supplement 1 (2018): 2364. http://dx.doi.org/10.1182/blood-2018-99-116742.

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Abstract Introduction: Sickle cell disease (SCD) is an autosomal-recessive-genetic disorder, which leads to red blood cell sickling, hemolysis and vaso-occlusion. Acute systemic painful vaso-occlusive crisis (VOC) is the predominant pathophysiology requiring emergency medical care by SCD patients. 10-20% of SCD patients hospitalized with VOC tend to develop acute chest syndrome (ACS), a type of lung injury within next few days, suggesting a role for pulmonary vaso-occlusion in ACS. This epidemiology also provides a window for therapeutic intervention provided treatments to prevent vaso-occlusi

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Pradhan, Tirthadipa, Prithu Sundd, Mark T. Gladwin, Satdarshan Pal Monga, and Gregory J. Kato. "Impaired Bile Secretion Promotes Chronic Liver Injury in Sickle Cell Disease." Blood 134, Supplement_1 (2019): 3536. http://dx.doi.org/10.1182/blood-2019-131915.

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Hepatic crisis is an emergent complication affecting sickle cell disease (SCD) patients, however, the molecular mechanism of sickle cell hepatobiliary crisis remains poorly understood. We examined the liver pathophysiology of SCD using a humanized mouse model (townes SCD mice; homozygous for Hbatm1(HBA)Tow, homozygous for Hbbtm2(HBG1,HBB*)Tow). These mice have the major features (irreversibly sickled red cells, splenomegaly, anemia, multiorgan pathology) found in humans with SCD and, as such, represent a useful in vivo system to study hepatobiliary changes in SCD disease. SCD mice manifested p

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Kaminski, Tomasz W., Tomasz Brzoska, Egemen Tutuncuoglu, Margaret V. Ragni, and Prithu Sundd. "Neutrophil Extracellular Traps Promote Joint Injury in Hemophilia." Blood 138, Supplement 1 (2021): 990. http://dx.doi.org/10.1182/blood-2021-153645.

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Abstract Epidemiological evidence suggests that recurring episodes of joint-bleeding contribute to the development of hemophilic arthropathy (H) in 70-85% of hemophilia patients. Despite major advances in the treatment to prevent joint bleeding, HA continues to be a major morbidity affecting hemophilia patients and the etiological mechanism contributing to the progression of HA remains poorly understood. Recent evidence suggests that the accumulation of blood in the joints may lead to the release of erythrocyte-derived DAMPs (eDAMPs) such as heme and hemoglobin that can promote sterile inflamm

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Lu, Qiang, Shaoqun Zeng, Qingming Luo, and Yu Ruan. "Rapid modeling of two-dimensional multi-photon excitation microscopic imaging through turbid medium." Optics Communications 189, no. 4-6 (2001): 227–34. http://dx.doi.org/10.1016/s0030-4018(01)01033-1.

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Vats, Ravi, Egemen Tutuncuoglu, Jesus Tejero, Cheryl A. Hillery, Mark T. Gladwin, and Prithu Sundd. "Circulating Neutrophil Extracellular Traps in the Pathogenesis of Acute Chest Syndrome of Sickle Cell Disease." Blood 134, Supplement_1 (2019): 3556. http://dx.doi.org/10.1182/blood-2019-130574.

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Introduction: Acute chest syndrome (ACS) is a type of acute lung injury and among the primary reasons for mortality and morbidity among Sickle Cell Disease (SCD) patients. Although epidemiologic evidence suggests that vaso-occlusion in the lung may serve as an antecedent to ACS, the cellular, molecular and biophysical mechanism of ACS is incompletely understood. Our recent findings revealed that the lung vaso-occlusion is enabled by the entrapment of embolic neutrophil-platelet aggregates in the pulmonary arterioles of transgenic humanized SCD mice. Recent evidence also suggests a role for neu

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Santos, Francisco A., Carlos E. R. Cardoso, José J. Rodrigues, Leonardo De Boni, and Luis M. G. Abegão. "Nonlinear Optical Materials: Predicting the First-Order Molecular Hyperpolarizability of Organic Molecular Structures." Photonics 10, no. 5 (2023): 545. http://dx.doi.org/10.3390/photonics10050545.

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Experimental nonlinear optics (NLO) is usually expensive due to the high-end photonics and electronic devices needed to perform experiments such as incoherent second harmonic generation in liquid phase, multi-photon absorption, and excitation. Nevertheless, exploring NLO responses of organic and inorganic compounds has already opened a world of new possibilities. For example, NLO switches, NLO frequency converters, and a new way to obtain biological images through the incoherent second harmonic generation (SHG) originate from first-order molecular hyperpolarizability (β). The microscopic effec

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Cheng, P. C., J. H. Chen, S. C. Hwang, C. K. Sun, D. B. Walden, and W. Y. Cheng. "3D Visualization of Maize Stem by MRI Technology." Microscopy and Microanalysis 7, S2 (2001): 100–101. http://dx.doi.org/10.1017/s143192760002657x.

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Recent development in confocal and multi-photon microscopy allows 3D imaging of plant tissue in high resolution. However, other than physical sectioning, macroscopical study of plant organs in 3D remains a difficult task. Among various available technologies for macroscopical imaging (e.g., Xray macro-tomography, optical coherent tomography and MRI), MRI is an ideal choice for its contrasting modality in volumetric imaging of soft tissues. A 3T Biospect MRI system (Brucker, Germany)(FIG 1) equipped with a 6cm inner diameter micro-quadrature coil (FIG 2) for RF transmission and reception of MRI

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Diaspro, Alberto, Paolo Bianchini, Giuseppe Vicidomini, Mario Faretta, Paola Ramoino, and Cesare Usai. "Multi-photon excitation microscopy." BioMedical Engineering OnLine 5, no. 1 (2006). http://dx.doi.org/10.1186/1475-925x-5-36.

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Hoang, Van Thuy, Yassin Boussafa, Lynn Sader, Sébastien Février, Vincent Couderc, and Benjamin Wetzel. "Optimizing supercontinuum spectro-temporal properties by leveraging machine learning towards multi-photon microscopy." Frontiers in Photonics 3 (September 6, 2022). http://dx.doi.org/10.3389/fphot.2022.940902.

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Multi-photon microscopy has played a significant role in biological imaging since it allows to observe living tissues with improved penetration depth and excellent sectioning effect. Multi-photon microscopy relies on multi-photon absorption, enabling the use of different imaging modalities that strongly depends on the properties of the sample structure, the selected fluorophore and the excitation laser. However, versatile and tunable laser excitation for multi-photon absorption is still a challenge, limited by e.g. the narrow bandwidth of typical laser gain medium or by the tunability of wavel

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Kamada, Takafumi, Kohei Otomo, Takashi Murata, et al. "Low-invasive 5D visualization of mitotic progression by two-photon excitation spinning-disk confocal microscopy." Scientific Reports 12, no. 1 (2022). http://dx.doi.org/10.1038/s41598-021-04543-7.

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AbstractNon-linear microscopy, such as multi-photon excitation microscopy, offers spatial localities of excitations, thereby achieving 3D cross-sectional imaging with low phototoxicity even in thick biological specimens. We had developed a multi-point scanning two-photon excitation microscopy system using a spinning-disk confocal scanning unit. However, its severe color cross-talk has precluded multi-color simultaneous imaging. Therefore, in this study, we introduced a mechanical switching system to select either of two NIR laser light pulses and an image-splitting detection system for 3- or 4

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Maity, Barun Kumar, and Sudipta Maiti. "Label-free imaging of neurotransmitters in live brain tissue by multi-photon ultraviolet microscopy." Neuronal Signaling 2, no. 4 (2018). http://dx.doi.org/10.1042/ns20180132.

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Visualizing small biomolecules in living cells remains a difficult challenge. Neurotransmitters provide one of the most frustrating examples of this difficulty, as our understanding of signaling in the brain critically depends on our ability to follow the neurotransmitter traffic. Last two decades have seen considerable progress in probing some of the neurotransmitters, e.g. by using false neurotransmitter mimics, chemical labeling techniques, or direct fluorescence imaging. Direct imaging harnesses the weak UV fluorescence of monoamines, which are some of the most important neurotransmitters

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