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Artykuły w czasopismach na temat „Multiplex screening”

Autor: Grafiati

Data publikacji: 2 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Brown, Stephen. "Marketing as Multiplex: Screening Postmodernism." European Journal of Marketing 28, no. 8/9 (1994): 27–51. http://dx.doi.org/10.1108/03090569410067631.

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Beadling, Carol, Michael C. Heinrich, Andrea Warrick, et al. "Multiplex Mutation Screening by Mass Spectrometry." Journal of Molecular Diagnostics 13, no. 5 (2011): 504–13. http://dx.doi.org/10.1016/j.jmoldx.2011.04.003.

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Berthuy, Ophélie I., Loïc J. Blum, and Christophe A. Marquette. "Cells on chip for multiplex screening." Biosensors and Bioelectronics 76 (February 2016): 29–37. http://dx.doi.org/10.1016/j.bios.2015.04.024.

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Shi, Run Zhang, Joseph M. Morrissey, and Janet D. Rowley. "Screening and Quantification of Multiple Chromosome Translocations in Human Leukemia." Clinical Chemistry 49, no. 7 (2003): 1066–73. http://dx.doi.org/10.1373/49.7.1066.

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Abstract Background: Characterization of fusion gene transcripts in leukemia that result from chromosome translocations provides valuable information regarding appropriate treatment and prognosis. However, screening for multiple fusion gene transcripts is difficult with conventional PCR and state-of-the-art real-time PCR and high-density microarrays. Methods: We developed a multiplex reverse transcription-PCR (RT-PCR) assay for screening and quantification of fusion gene transcripts in human leukemia cells. Chimeric primers were used that contained gene-specific and universal sequences. PCR am

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Berthuy, Ophélie I., Sinan K. Muldur, François Rossi, Pascal Colpo, Loïc J. Blum, and Christophe A. Marquette. "Multiplex cell microarrays for high-throughput screening." Lab on a Chip 16, no. 22 (2016): 4248–62. http://dx.doi.org/10.1039/c6lc00831c.

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Mannerlöf, Marie, and Paul Tenning. "Screening of transgenic plants by multiplex PCR." Plant Molecular Biology Reporter 15, no. 1 (1997): 38–45. http://dx.doi.org/10.1007/bf02772111.

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Grad, Alecsandra Iulia, Mihaela Laura Vica, Loredana Ungureanu, Costel Vasile Siserman, Alexandru Dumitru Tătaru, and Horea Vladi Matei. "Assessment of STI screening in Romania using a multiplex PCR technique." Journal of Infection in Developing Countries 14, no. 04 (2020): 341–48. http://dx.doi.org/10.3855/jidc.11989.

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Introduction: Most sexually transmitted infections (STIs) are curable, but inappropriate treatment can lead to serious complications. The importance of setting up STI screening programs has been highlighted in various studies, the absence of such national programs accounting for the lack of STI statistics in Romania. The purpose of our study was to evaluate multiplex PCR as a screening method for the most common 6 STIs and establish their frequency in a group of symptomatic and asymptomatic patients. We aimed to highlight STI associations and correlations between STI pathogens and symptomatolo

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He, Ling, Xiaofan Jia, Yong Gu, et al. "Large-Scale Screening in General Population Children for Celiac Disease with a Multiplex Electrochemiluminescence (ECL) Assay." Journal of Immunology Research 2020 (December 24, 2020): 1–6. http://dx.doi.org/10.1155/2020/8897656.

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Background. Autoimmunity Screening for Kids (ASK) study was launched to screen general population children for type 1 diabetes (T1D) and celiac disease (CD). Methods. A total of 23,319 children from general population were screened. A high throughput multiplex electrochemiluminescence (ECL) assay to screen multiautoantibodies in a single well was applied, parallel with a standard radiobinding assay (RBA). All children with any positive autoantibodies in screening were revisited within one month for confirmation and followed every 6 months. Results. Among 23,319 children, 2.6% (606/23,319) of c

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Rathore, Rakesh, Patrick Pribil, Jay J. Corr, William L. Seibel, Artem Evdokimov, and Kenneth D. Greis. "Multiplex Enzyme Assays and Inhibitor Screening by Mass Spectrometry." Journal of Biomolecular Screening 15, no. 8 (2010): 1001–7. http://dx.doi.org/10.1177/1087057110363824.

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Current methods for high-throughput screening (HTS) use a serial process to evaluate compounds as inhibitors toward a single therapeutic target, but as the demand to reduce screening time and cost continues to grow, one solution is the development of multiplex technology. In this communication, the multiplex assay capability of a mass spectrometry (MS)–based readout system is verified using a kinase and esterase reaction simultaneously. Furthermore, the MS-based readout is shown to be compatible with a typical HTS workflow by identifying and validating several new inhibitors for each enzyme fr

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Li, Ning, Feika Bian, Xiaowei Wei, et al. "Pollen-Inspired Photonic Barcodes with Prickly Surface for Multiplex Exosome Capturing and Screening." Research 2022 (September 1, 2022): 1–9. http://dx.doi.org/10.34133/2022/9809538.

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Exosomes, which play an important role in intercellular communication, are closely related to the pathogenesis of disease. However, their effective capture and multiplex screening are still challenging. Here, inspired by the unique structure of pollens, we present novel photonic crystal (PhC) barcodes with prickly surface by hydrothermal synthesis for multiplex exosome capturing and screening. These pollen-inspired PhC barcodes are imparted with extremely high specific surface area and excellent prickly surface nanostructures, which can improve the capture rate and detection sensitivity of exo

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Hou, Peijie, Joshua M. Tebbs, Dewei Wang, Christopher S. McMahan, and Christopher R. Bilder. "Array testing for multiplex assays." Biostatistics 21, no. 3 (2018): 417–31. http://dx.doi.org/10.1093/biostatistics/kxy058.

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Summary Group testing involves pooling individual specimens (e.g., blood, urine, swabs, etc.) and testing the pools for the presence of disease. When the proportion of diseased individuals is small, group testing can greatly reduce the number of tests needed to screen a population. Statistical research in group testing has traditionally focused on applications for a single disease. However, blood service organizations and large-scale disease surveillance programs are increasingly moving towards the use of multiplex assays, which measure multiple disease biomarkers at once. Tebbs and others (20

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Gaudin, Valérie. "The Growing Interest in Development of Innovative Optical Aptasensors for the Detection of Antimicrobial Residues in Food Products." Biosensors 10, no. 3 (2020): 21. http://dx.doi.org/10.3390/bios10030021.

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The presence of antimicrobial residues in food-producing animals can lead to harmful effects on the consumer (e.g., allergies, antimicrobial resistance, toxicological effects) and cause issues in food transformation (i.e., cheese, yogurts production). Therefore, to control antimicrobial residues in food products of animal origin, screening methods are of utmost importance. Microbiological and immunological methods (e.g., ELISA, dipsticks) are conventional screening methods. Biosensors are an innovative solution for the development of more performant screening methods. Among the different kinds

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Stedtfeld, Robert D., Sam Baushke, Dieter Tourlousse, Benli Chai, James R. Cole, and Syed A. Hashsham. "Multiplex Approach for Screening Genetic Markers of Microbial Indicators." Water Environment Research 79, no. 3 (2007): 260–69. http://dx.doi.org/10.2175/106143007x181378.

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Dillon, B., L. Thomas, G. Mohmand, A. Zelynski, and J. Iredell. "Multiplex PCR for screening of integrons in bacterial lysates." Journal of Microbiological Methods 62, no. 2 (2005): 221–32. http://dx.doi.org/10.1016/j.mimet.2005.02.007.

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Martínez-Glez, Víctor, Carmen Franco-Hernández, Jesús Lomas, et al. "Multiplex ligation-dependent probe amplification (MLPA) screening in meningioma." Cancer Genetics and Cytogenetics 173, no. 2 (2007): 170–72. http://dx.doi.org/10.1016/j.cancergencyto.2006.09.011.

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Marras, Salvatore A. E., Sanjay Tyagi, Dan-Oscar Antson, and Fred Russell Kramer. "Color-coded molecular beacons for multiplex PCR screening assays." PLOS ONE 14, no. 3 (2019): e0213906. http://dx.doi.org/10.1371/journal.pone.0213906.

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Park, Jung Hun, Ki Soo Park, Kyungmee Lee, Hyowon Jang, and Hyun Gyu Park. "Universal probe amplification: Multiplex screening technologies for genetic variations." Biotechnology Journal 10, no. 1 (2014): 45–55. http://dx.doi.org/10.1002/biot.201400219.

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Eastwood, Niamh, and Luisa Orsini. "Are multiplexed metabarcoding panels comparable to individual marker gene library preparations?" ARPHA Conference Abstracts 4 (March 4, 2021): e64895. https://doi.org/10.3897/aca.4.e64895.

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Analysis of multiple marker genes using metabarcoding of environmental DNA (eDNA) can offer information greater than that from sequencing single marker genes, such as responses from across the phylogenetic tree to environmental gradients (Cordier et al. 2019). Furthermore, multiple regions of the same gene can be sequenced to improve phylogenetic resolution (Fuks et al. 2018). However, separate amplification reactions and library preparation steps for each marker can be costly and time consuming.Here, we have designed and optimised a multiplex panel of four marker genes (two regions of 18S rRN

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Roman, David L., Shodai Ota, and Richard R. Neubig. "Polyplexed Flow Cytometry Protein Interaction Assay: A Novel High-Throughput Screening Paradigm for RGS Protein Inhibitors." Journal of Biomolecular Screening 14, no. 6 (2009): 610–19. http://dx.doi.org/10.1177/1087057109336590.

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Intracellular signaling cascades are a series of regulated protein-protein interactions that may provide a number of targets for potential drug discovery. Here, the authors examine the interaction of regulators of G-protein signaling (RGS) proteins with the G-protein Gαo, using a flow cytometry protein interaction assay (FCPIA). FCPIA accurately measures nanomolar binding constants of this protein-protein interaction and has been used in high-throughput screening. This report focuses on 5 RGS proteins (4, 6, 7, 8, and 16). To increase the content of screens, the authors assessed high-throughpu

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Yun, Seung Gyu, Jin Woo Jang, Jong Han Lee, et al. "Evaluation of Novel Multiplex Antibody Kit for Human Immunodeficiency Virus 1/2 and Hepatitis C Virus Using Sol-Gel Based Microarray." BioMed Research International 2015 (2015): 1–7. http://dx.doi.org/10.1155/2015/837296.

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Background. Microarrays enable high-throughput screening (HTS) of disease-related molecules, including important signaling proteins/peptides and small molecules that are in low abundance. In this study, we developed a multiplex blood bank screening platform, referred to as the Hi3-1 assay, for simultaneous detection of human immunodeficiency virus 1/2 (HIV 1/2) and hepatitis C virus (HCV).Methods. The Hi3-1 assay was tested using four panels (Panel 1,n=4,581patient samples; Panel 2,n=15seroconversion samples; Panel 3,n=4performance samples; and Panel 4,n=251purchased positive control samples),

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Gerstel-Thompson, Jacalyn L., Jonathan F. Wilkey, Jennifer C. Baptiste, et al. "High-Throughput Multiplexed T-Cell–Receptor Excision Circle Quantitative PCR Assay with Internal Controls for Detection of Severe Combined Immunodeficiency in Population-Based Newborn Screening." Clinical Chemistry 56, no. 9 (2010): 1466–74. http://dx.doi.org/10.1373/clinchem.2010.144915.

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BACKGROUND Real-time quantitative PCR (qPCR) targeting a specific marker of functional T cells, the T-cell–receptor excision circle (TREC), detects the absence of functional T cells and has a demonstrated clinical validity for detecting severe combined immunodeficiency (SCID) in infants. There is need for a qPCR TREC assay with an internal control to monitor DNA quality and the relative cellular content of the particular dried blood spot punch sampled in each reaction. The utility of the qPCR TREC assay would also be far improved if more tests could be performed on the same newborn screening s

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Quthami, Khalid AL, Fadel Qabani, Saeedi Mazen G, Abusaadh Fauziah F, Esmat Suliman A, and Badahdah Saeed A. "Evaluation of the Roche Cobas TaqScreen Multiplex V 2.0 Test for Blood Screening: A Saudi Arabian Study." International Journal of Sciences Volume 4, no. 2015-02 (2015): 26–30. https://doi.org/10.5281/zenodo.3348851.

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Clinical specificity and genotype/subtype detection of viruses using the Cobas TaqScreen MPX system V 2.0, which is a nucleic acid test (NAT) that uses multiples for hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus (HIV) strain variants, were investigated. The 95% LOD was employed in the experiment and the results returned a positive accuracy of 99.6%. which is consistent with the expected success rate as specified by Roche.Read Complete Article at ijSciences: V4201501620

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Qu, Jianwen, Huijuan Xie, Shuying Zhang, et al. "Multiplex Flow Cytometric Immunoassays for High-Throughput Screening of Multiple Mycotoxin Residues in Milk." Food Analytical Methods 12, no. 4 (2019): 877–86. http://dx.doi.org/10.1007/s12161-018-01412-4.

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Savira, Maya, Enikarmila Asni, and Rahmat Azhari Kemal. "Optimization of multiplex PCR composition to screen for SARS-CoV-2 variants of concern." Acta Biochimica Indonesiana 4, no. 2 (2021): 58. http://dx.doi.org/10.32889/actabioina.58.

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Background: The ongoing COVID-19 pandemic has led to the emergence of several variants of concern. To rapidly identify those variants, screening samples for whole-genome sequencing (WGS) prioritization could be performed.  Objective: We optimized the polymerase chain reaction (PCR) screening method to identify the mutation in spike and ORF1a regions.  Methods: We adopted primers targeting mutation in spike and ORF1a region from another study. We optimized the PCR screening method using kits readily available in Indonesia. Firstly, we compared N1 and N2 primers as internal positive

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Pastinen, T., J. Partanen, and A. C. Syvänen. "Multiplex, fluorescent, solid-phase minisequencing for efficient screening of DNA sequence variation." Clinical Chemistry 42, no. 9 (1996): 1391–97. http://dx.doi.org/10.1093/clinchem/42.9.1391.

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Abstract We developed a multiplex, solid-phase minisequencing method to detect multiple single-nucleotide polymorphisms in an undivided sample. The amplified DNA templates are first captured on a manifold. Then, with multiple minisequencing primers of various sizes, single-nucleotide extension reactions are carried out simultaneously with fluorescently labeled dideoxynucleotides. The size of the extended product, determined by using a DNA sequencing instrument, defines the site of the polymorphisms, and the incorporated nucleotide gives the identity of the nucleotide at each site. HLA-DQA1 typ

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Tung, Hsiang-Yun, Wei-Chen Chen, Bor-Rung Ou, et al. "Simultaneous detection of multiple pathogens by multiplex PCR coupled with DNA biochip hybridization." Laboratory Animals 52, no. 2 (2017): 186–95. http://dx.doi.org/10.1177/0023677217718864.

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Traditional serological enzyme-linked immunosorbent assay (ELISA) is routinely used to monitor pathogens during quarantine in most animal facilities to prevent possible infection. However, the ELISA platform is a single-target assay, and screening all targeted pathogens is time-consuming and laborious. In this study, to increase sensitivity and to reduce diagnosis time for high-throughput processes, multiplex PCR and DNA biochip techniques were combined to develop a multi-pathogen diagnostic method for use instead of routine ELISA. Eight primer sets were designed for multiplex PCR to detect ge

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Lim, Jihyang, Myungshin Kim, Yonggoo Kim, et al. "Analysis of Genetic Alterations In 843 Korean Acute Leukemia Patients by Multiplex RT-PCR Assay (HemaVision) and Karyotyping." Blood 116, no. 21 (2010): 4845. http://dx.doi.org/10.1182/blood.v116.21.4845.4845.

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Abstract Abstract 4845 Background: The importance of molecular tests has been increased for diagnosis, classification and MRD monitoring in acute leukemia. Recently, multiplex RT-PCR assays (HemaVision) for simultaneous detection of 28 leukemia-associated translocations was introduced and has been used for molecular screening of leukemic patients. We analyzed the results of multiplex RT-PCR assay (HemaVision) and karyotyping in Korean acute leukemia patients. Methods: From April 2007 to March 2010, multiplex RT-PCR assay (HemaVision, DNA Technology A/S, Denmark) was performed in 843 Korean acu

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Pretto, Dalyir, Dianna Maar, Carolyn M. Yrigollen, Jack Regan, and Flora Tassone. "Screening Newborn Blood Spots for 22q11.2 Deletion Syndrome Using Multiplex Droplet Digital PCR." Clinical Chemistry 61, no. 1 (2015): 182–90. http://dx.doi.org/10.1373/clinchem.2014.230086.

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Abstract BACKGROUND The diagnosis of 22q11 deletion syndrome (22q11DS) is often delayed or missed due to the wide spectrum of clinical involvement ranging from mild to severe, often life-threatening conditions. A delayed diagnosis can lead to life-long health issues that could be ameliorated with early intervention and treatment. Owing to the high impact of 22q11DS on public health, propositions have been made to include 22q11DS in newborn screening panels; however, the method of choice for detecting 22q11DS, fluorescent in situ hybridization, requires specialized equipment and is cumbersome f

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Maitra, Radhashree, Jay B. Nayak, Atrayee Basu-Mallick, et al. "Rapid screening of SNPs in metastatic colorectal cancer (mCRC) utilizing multiplex sequencing technology (Sequenom)." Journal of Clinical Oncology 30, no. 4_suppl (2012): 418. http://dx.doi.org/10.1200/jco.2012.30.4_suppl.418.

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418 Background: Accurate and fast screening of mutations is essential for designing individualized therapy necessary and critical for efficient disease management and better patient outcome in mCRC. Detection of hotspots by gold standard direct sequencing (DS) is time consuming and cost ineffective. Pyrosequencing (PS) technique is rapid and precisely committed towards SNP detection. Recent introduction of high throughput multiplex PCR based extension on microarray (Sequenom, SEQ) offers a robust platform capable of detecting multiple SNPs simultaneously in a rapid and cost effective manner. T

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Cairns, Lauren V., Katrina M. Lappin, Alexander Mutch, Ahlam Ali, Kyle B. Matchett, and Ken I. Mills. "Multiplex Screening for Interacting Compounds in Paediatric Acute Myeloid Leukaemia." International Journal of Molecular Sciences 22, no. 18 (2021): 10163. http://dx.doi.org/10.3390/ijms221810163.

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Paediatric acute myeloid leukaemia (AML) is a heterogeneous disease characterised by the malignant transformation of myeloid precursor cells with impaired differentiation. Standard therapy for paediatric AML has remained largely unchanged for over four decades and, combined with inadequate understanding of the biology of paediatric AML, has limited the progress of targeted therapies in this cohort. In recent years, the search for novel targets for the treatment of paediatric AML has accelerated in parallel with advanced genomic technologies which explore the mutational and transcriptional land

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Schillberg, Stefan, Detlef Schumann, and Rainer Fischer. "PCR-Based Multiplex Method for Rapid Screening of Recombinant Bacteria." BioTechniques 23, no. 2 (1997): 212–16. http://dx.doi.org/10.2144/97232bm06.

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Kang, Guhyun, Jeeyun Lee, Ki Taek Jang, et al. "Multiplex mutation screening by mass spectrometry in gastrointestinal stromal tumours." Pathology 44, no. 5 (2012): 460–64. http://dx.doi.org/10.1097/pat.0b013e3283559c45.

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Cheng Pettersson, Angela, Anita Viskari, Ulrika Odén, et al. "Improved MPL mutation screening with multiplex PCR and capillary electrophoresis." British Journal of Haematology 179, no. 5 (2016): 838–40. http://dx.doi.org/10.1111/bjh.14253.

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Ben-Dov, Eitan, Qingfeng Wang, Arieh Zaritsky, et al. "Multiplex PCR Screening To Detect cry9Genes in Bacillus thuringiensis Strains." Applied and Environmental Microbiology 65, no. 8 (1999): 3714–16. http://dx.doi.org/10.1128/aem.65.8.3714-3716.1999.

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ABSTRACT An extended PCR method was established to rapidly identify and classify Bacillus thuringiensis strains containingcry (crystal protein) genes toxic to lepidopteran, coleopteran, and dipteran pests (Ben-Dov et al., Appl. Environ. Microbiol. 63:4883–4890, 1997). To optimize identification of all reported cry genes, this methodology needs a complete PCR set of primers. In the study reported here, a set of universal (Un9) and specific primers for multiplex rapid screening for all four known genes from the cry9 group was designed. PCR analyses were performed for cry9 genes on 16 standard st

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Udaka, Toru, Issei Imoto, Yoshinori Aizu, et al. "Multiplex PCR/Liquid Chromatography Assay for Screening of Subtelomeric Rearrangements." Genetic Testing 11, no. 3 (2007): 241–48. http://dx.doi.org/10.1089/gte.2007.9993.

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Emi, Mitsuru, Mieko Matsushima, Toyomasa Katagiri, et al. "Multiplex Mutation Screening of theBRCA1Gene in 1000 Japanese Breast Cancers." Japanese Journal of Cancer Research 89, no. 1 (1998): 12–16. http://dx.doi.org/10.1111/j.1349-7006.1998.tb00472.x.

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Andersen, Rikke Fredslund, and Anders Jakobsen. "Screening for circulating RAS/RAF mutations by multiplex digital PCR." Clinica Chimica Acta 458 (July 2016): 138–43. http://dx.doi.org/10.1016/j.cca.2016.05.007.

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Oliveira, Beatriz B., Bruno Veigas, Fábio Ferreira Carlos, et al. "Water safety screening via multiplex LAMP-Au-nanoprobe integrated approach." Science of The Total Environment 741 (November 2020): 140447. http://dx.doi.org/10.1016/j.scitotenv.2020.140447.

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Chandrasekar, A., K. Kalaiponmani, S. Elayabalan, K. K. Kumar, K. Angappan, and P. Balasubramanian. "Screening of banana bunchy top virus through multiplex PCR approach." Archives Of Phytopathology And Plant Protection 44, no. 19 (2011): 1920–25. http://dx.doi.org/10.1080/03235408.2010.522820.

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Takeuchi, Kengo, Young Lim Choi, Manabu Soda, et al. "Multiplex Reverse Transcription-PCR Screening for EML4-ALK Fusion Transcripts." Clinical Cancer Research 14, no. 20 (2008): 6618–24. http://dx.doi.org/10.1158/1078-0432.ccr-08-1018.

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Picci, Luigi, Franca Anglani, Maurizio Scarpa, and Franco Zacchello. "Screening for cystic fibrosis gene mutations by multiplex DNA amplification." Human Genetics 88, no. 5 (1992): 552–56. http://dx.doi.org/10.1007/bf00219343.

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Naghi, Leah A., Julie O. Culver, Charité Ricker, et al. "Breast Cancer MRI Screening of Patients After Multiplex Gene Panel Testing." JAMA Network Open 8, no. 1 (2025): e2454447. https://doi.org/10.1001/jamanetworkopen.2024.54447.

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ImportanceEnhanced breast cancer screening with magnetic resonance imaging (MRI) is recommended to women with elevated risk of breast cancer, yet uptake of screening remains unclear after genetic testing.ObjectiveTo evaluate uptake of MRI after genetic results disclosure and counseling.Design, Setting, and ParticipantsThis multicenter cohort study was conducted at the University of Southern California Norris Cancer Hospital, the Los Angeles General Medical Center, and the Stanford University Cancer Institute. Patients were recruited from July 1, 2014, through November 30, 2016. Following multi

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Cho, Young-Uk, Hyun-Sook Chi, Seongsoo Jang, et al. "The Molecular Screening Using Multiplex Reverse Transcription Polymerase Chain Reaction and the Comparison with Cytogenetic Analysis in the Prognostic Stratification of Acute Leukemia." Blood 112, no. 11 (2008): 4868. http://dx.doi.org/10.1182/blood.v112.11.4868.4868.

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Abstract INTRODUCTION: The detection of chromosomal aberrations is essential for the diagnosis and prognostic stratification of acute leukemia. Classical cytogenetic analysis has the advantage of comprehensive screening of all types of abnormalities such as numerical aberrations and deletions as well as translocations. However, this technique is time-consuming and labor-intensive, and cannot detect cryptic translocations. Recently, a multiplex reverse transcription polymerase chain reaction (multiplex RT-PCR) has emerged as a tool for overcoming disadvantages of cytogenetic analysis with some

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Peña, Jeremy Ryan A., and Robert S. Makar. "Routine Solid Phase Multiplex Anti-HLA Antibody Tests Predict Platelet Refractoriness." American Journal of Clinical Pathology 152, no. 2 (2019): 146–54. http://dx.doi.org/10.1093/ajcp/aqz024.

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ABSTRACTObjectivesNo validated screening methods identify patients at risk for human leukocyte antigen (HLA) alloimmune-mediated platelet refractoriness (alloPR). We determined if bead-based HLA antibody tests could predict risk of developing HLA alloPR.MethodsHematopoietic progenitor cell transplant patients screened for HLA antibodies without prior refractoriness were identified. Phenotype bead screening results were compared between patients who later did and did not develop alloPR.ResultsSeven of 27 patients identified subsequently developed alloPR. The panel reactive antibody (PRA) and me

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Zhang, X. Kate, Carole S. Elbin, Wei-Lien Chuang, et al. "Multiplex Enzyme Assay Screening of Dried Blood Spots for Lysosomal Storage Disorders by Using Tandem Mass Spectrometry." Clinical Chemistry 54, no. 10 (2008): 1725–28. http://dx.doi.org/10.1373/clinchem.2008.104711.

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Abstract Background: Reports of the use of multiplex enzyme assay screening for Pompe disease, Fabry disease, Gaucher disease, Niemann-Pick disease types A and B, and Krabbe disease have engendered interest in the use of this assay in newborn screening. We modified the assay for high-throughput use in screening laboratories. Methods: We optimized enzyme reaction conditions and procedures for the assay, including the concentrations of substrate (S) and internal standard (IS), assay cocktail compositions, sample clean-up procedures, and mass spectrometer operation. The S and IS for each enzyme w

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Yelliantty, Yelliantty, and Ira Endah Rohima. "Analysis of Species in Meat Products in Bandung City using Multiplex PCR." Pasundan Food Technology Journal 4, no. 3 (2018): 215. http://dx.doi.org/10.23969/pftj.v4i3.658.

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Cases concerning the substitution of meat raw material also occur in Indonesia and are quite common. Therefore, careful monitoring and control that needs to be done on the meat products. Screening or sampling products on the market should be conducted periodically to ensure the safety of consumers and society in general. Such screening should be done accurately. This study aimed to analyze the composition of meat in processed products in traditional markets in Bandung using PCR method. This study was using four specific primers to detect four different species. Screening is done on samples of

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Lee, Mijin, İsa Taş, Rui Zhou, Sang-Chul Jung, Kwonseop Kim та Hangun Kim. "Development of a Multiplex Bead-Based Method for the Microquantitation of δ-Catenin". Journal of Nanoscience and Nanotechnology 20, № 9 (2020): 5819–22. http://dx.doi.org/10.1166/jnn.2020.17673.

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δ-Catenin is overexpressed in human cancers, including prostate, breast, lung, and ovarian cancers. Therefore, detection of δ-catenin level in patient specimens can be used as a diagnostic marker for the cancer screening. In laboratories, δ-catenin levels have been analyzed by western blot, which requires multiple procedures and is incapable of multiplex analysis of a target protein from a single reaction. In this study, we aimed to develop δ-catenin antibody-conjugated magnetic beads that can be used for quantitation of δ-catenin by bead-based multiplex assay. δ-catenin level from HEK293T and

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Mishina, Yuji M., Christopher J. Wilson, Linda Bruett, et al. "Multiplex GPCR Assay in Reverse Transfection Cell Microarrays." Journal of Biomolecular Screening 9, no. 3 (2004): 196–207. http://dx.doi.org/10.1177/1087057103261880.

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G protein-coupled receptors (GPCRs) are a superfamily of proteins that include some of the most important drug targets in the pharmaceutical industry. Despite the success of this group of drugs, there remains a need to identify GPCR-targeted drugs with greater selectivity, to develop screening assays for validated targets, and to identify ligands for orphan receptors. To address these challenges, the authors have created a multiplexed GPCR assay that measures greater than 3000 receptor: ligand interactions in a single microplate. The multiplexed assay is generated by combining reverse transfec

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Chen, Fumin, Qinqin Hu, Huimin Li, et al. "Multiplex Detection of Infectious Diseases on Microfluidic Platforms." Biosensors 13, no. 3 (2023): 410. http://dx.doi.org/10.3390/bios13030410.

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Infectious diseases contribute significantly to the global disease burden. Sensitive and accurate screening methods are some of the most effective means of identifying sources of infection and controlling infectivity. Conventional detecting strategies such as quantitative polymerase chain reaction (qPCR), DNA sequencing, and mass spectrometry typically require bulky equipment and well-trained personnel. Therefore, mass screening of a large population using conventional strategies during pandemic periods often requires additional manpower, resources, and time, which cannot be guaranteed in reso

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Liu, Yang, Fan Yi, Arun Babu Kumar, et al. "Multiplex Tandem Mass Spectrometry Enzymatic Activity Assay for Newborn Screening of the Mucopolysaccharidoses and Type 2 Neuronal Ceroid Lipofuscinosis." Clinical Chemistry 63, no. 6 (2017): 1118–26. http://dx.doi.org/10.1373/clinchem.2016.269167.

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Abstract BACKGROUND We expanded the use of tandem mass spectrometry combined with liquid chromatography (LC-MS/MS) for multiplex newborn screening of seven lysosomal enzymes in dried blood spots (DBS). The new assays are for enzymes responsible for the mucopolysaccharidoses (MPS-I, -II, -IIIB, -IVA, -VI, and -VII) and type 2 neuronal ceroid lipofuscinosis (LINCL). METHODS New substrates were prepared and characterized for tripeptidyl peptidase 1 (TPP1), α-N-acetylglucosaminidase (NAGLU), and lysosomal β-glucuronidase (GUSB). These assays were combined with previously developed assays to provid

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