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Gotowe bibliografie tematyczne / Neutrophils and Red hepatization / Artykuły w czasopismach

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Artykuły w czasopismach na temat „Neutrophils and Red hepatization”

Autor: Grafiati

Data publikacji: 2 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Adedeji, S. O., E. O. Ogunba, and O. O. Dipeolu. "Synergistic effect of migrating Ascaris larvae and Escherichia coli in piglets." Journal of Helminthology 63, no. 1 (1989): 19–24. http://dx.doi.org/10.1017/s0022149x00008671.

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ABSTRACTThe effect of intestinal flora on the establishment, development and pathogenicity of Ascaris suum larvae in piglets (Large White breed) was investigated. The infected piglets with Ascaris and Escherichia coli showed signs of pneumonia, cough with respiratory difficulties initially even though these moderated with time. They lost appetite and showed signs of unthriftiness with loss of weight. The packed cell volume was normal but the differential leucocyte counts of the pigs infected with Ascaris larvae and bacteria had high neutrophils, unlike the very high lymphocyte count observed i

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2

Muralinath, E., D.V. Ramanjaneyulu, Pragna K. Sravani, et al. "An Essential Parameters of Pathology of Pneumonia Include Etiology, Epidemiology, Patho Physiology, Histo Pathology, Diagnosis, Differential Diagnosis and Treatment." Journal of Advanced Research and Reviews in Virology & Microbiology 2, no. 1 (2025): 40–48. https://doi.org/10.5281/zenodo.15486599.

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<em>Pneumonia is the general name for a collection of illnesses caused by different microorganisms that result in lung parenchymal infection. Treatment recommendations for effective therapy in both inpatient and outpatient settings have been developed with the use of categorization schemata, which have also helped identify the common pathogens specifically responsible for each kind of pneumonia. Enhance interprofessional team tactics to promote communication and care coordination in order to improve outcomes for patients affected by pneumonia.</em>

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Biswas, Deblina, Anshu Kumari, George C. K. Chen, et al. "Quantitative Differentiation of Pneumonia from Normal Lungs: Diagnostic Assessment Using Photoacoustic Spectral Response." Applied Spectroscopy 71, no. 11 (2017): 2532–37. http://dx.doi.org/10.1177/0003702817708320.

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Pneumonia is an acute lung infection that takes life of many young children in developing countries. Early stage (red hepatization) detection of pneumonia would be pragmatic to control mortality rate. Detection of this disease at early stages demands the knowledge of pathology, making it difficult to screen noninvasively. We propose photoacoustic spectral response (PASR), a noninvasive elasticity-dependent technique for early stage pneumonia detection. We report the quantitative red hepatization detection of pneumonia through median frequency, spectral energy, and variance. Significant contras

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4

Aoshiba, Kazutetsu, Yuri Nakajima, Shuji Yasui, Jun Tamaoki, and Atsushi Nagai. "Red Blood Cells Inhibit Apoptosis of Human Neutrophils." Blood 93, no. 11 (1999): 4006–10. http://dx.doi.org/10.1182/blood.v93.11.4006.411k18_4006_4010.

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Oxidative stress has been implicated in the triggering of apoptosis in neutrophils. Because red blood cells (RBCs) are well known to scavenge oxidants including H2O2, we tested the hypothesis that RBCs inhibit apoptosis of neutrophils by reducing intracellular oxidative stress. Apoptosis of neutrophils was evaluated by light microscopy and DNA gel electrophoresis. We found that coculture with RBCs protected against neutrophil apoptosis. Neither physical contact between RBCs and neutrophils nor the cellular integrity of RBCs was required to protect against neutrophil apoptosis. Neutrophil apopt

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5

Aoshiba, Kazutetsu, Yuri Nakajima, Shuji Yasui, Jun Tamaoki, and Atsushi Nagai. "Red Blood Cells Inhibit Apoptosis of Human Neutrophils." Blood 93, no. 11 (1999): 4006–10. http://dx.doi.org/10.1182/blood.v93.11.4006.

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Abstract Oxidative stress has been implicated in the triggering of apoptosis in neutrophils. Because red blood cells (RBCs) are well known to scavenge oxidants including H2O2, we tested the hypothesis that RBCs inhibit apoptosis of neutrophils by reducing intracellular oxidative stress. Apoptosis of neutrophils was evaluated by light microscopy and DNA gel electrophoresis. We found that coculture with RBCs protected against neutrophil apoptosis. Neither physical contact between RBCs and neutrophils nor the cellular integrity of RBCs was required to protect against neutrophil apoptosis. Neutrop

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6

Chin‐Yee, Keeney, Krueger, Dietz, and Moses. "Supernatant from stored red cells activates neutrophils." Transfusion Medicine 8, no. 1 (1998): 49–56. http://dx.doi.org/10.1046/j.1365-3148.1998.00125.x.

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7

Lautenschlager, Sueli de Oliveira Silva, Tehyung Kim, Danielle Lazarim Bidóia, Celso Vataru Nakamura, Hans-Joachim Anders, and Stefanie Steiger. "Plasma Proteins and Platelets Modulate Neutrophil Clearance of Malaria-Related Hemozoin Crystals." Cells 9, no. 1 (2019): 93. http://dx.doi.org/10.3390/cells9010093.

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Hemozoin is an insoluble crystalline pigment produced by the malaria parasite Plasmodia upon digesting host hemoglobin inside red blood cells. Red blood cell rupture releases hemozoin crystals into the circulation from where they are cleared by phagocytes such as neutrophils. We speculated that plasma proteins would affect the ability of neutrophils to clear hemozoin crystals. To test this, we cultured human blood neutrophils with hemozoin ex vivo and found that neutrophils ingested hemozoin (0.1–1 µm crystal size) in a dose-dependent manner into phagosomes and vesicles/vacuoles, resulting in

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8

Rhyan, J. C., and D. A. Saari. "A Comparative Study of the Histopathologic Features of Bovine Tuberculosis in Cattle, Fallow Deer (Dama dama), Sika Deer (Cervus nippon), and Red Deer and Elk (Cervus elaphus)." Veterinary Pathology 32, no. 3 (1995): 215–20. http://dx.doi.org/10.1177/030098589503200301.

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Sections of tuberculous lesions from 23 elk ( Cervus elaphus nelsoni) and red deer ( Cervus elaphus elaphus), 12 fallow deer ( Dama dama), 10 sika deer ( Cervus nippon), and 30 cattle were examined and compared. Lesions were scored for caseous necrosis, mineralization, neutrophils, macrophages, giant cells, and acid-fast bacilli. Some differences in lesion morphology between the species were noted. Elk/red deer lesions had marked variation and often differed from bovine lesions in several characteristics; elk/red deer lesions usually had scattered peripheral mineralization rather than central

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9

Ray, Debadrita, and Narender Kumar. "Red Blood Cells Garlanding Neutrophils: An Intriguing Observation." Journal of Postgraduate Medicine, Education and Research 59, no. 1 (2025): 39–40. https://doi.org/10.5005/jp-journals-10028-1677.

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10

Franco-Penteado, Carla Fernanda, Marcos André Cavalcanti Bezerra, Mariana R. B. Mello та ін. "Increased Adhesive Properties and Reactive Oxygen Species of Red Cells, Platelets and Leukocytes and Neutrophil Chemotaxis in IVS-1-6 (T → C) Homozygous β-Thalassemia Intermedia." Blood 112, № 11 (2008): 1873. http://dx.doi.org/10.1182/blood.v112.11.1873.1873.

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Abstract The pathophysiology of b-thalassemia intermedia (TI) is characterized by extravascular hemolysis, with the release into the peripheral circulation of damaged red blood cells and erythroid precursors. In these patients vascular complications and endothelial cell activation are more frequent and activation of vascular endothelium is considered an important aspect of inflammation, vasculitis and thrombosis in this disease. The mutation at position +6 of the IVS-1 5’ (donor) consensus sequence is particularly mild and is usually associated with TI owing to a relatively high level of norma

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11

Dávalos, Alberto, Gema de la Peña, Carolina C. Sánchez-Martín, M. Teresa Guerra, Begoña Bartolomé, and Miguel A. Lasunción. "Effects of red grape juice polyphenols in NADPH oxidase subunit expression in human neutrophils and mononuclear blood cells." British Journal of Nutrition 102, no. 8 (2009): 1125–35. http://dx.doi.org/10.1017/s0007114509382148.

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The NADPH oxidase enzyme system is the main source of superoxide anions in phagocytic and vascular cells. NADPH oxidase-dependent superoxide generation has been found to be abnormally enhanced in several chronic diseases. Evidence is accumulating that polyphenols may have the potential to improve cardiovascular health, although the mechanism is not fully established. Consumption of concentrated red grape juice, rich in polyphenols, has been recently shown to reduce NADPH oxidase activity in circulating neutrophils from human subjects. In the present work we studied whether red grape juice poly

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12

Jank, Herbert, and Ulrich Salzer. "Vesicles Generated during Storage of Red Blood Cells Enhance the Generation of Radical Oxygen Speciesin Activated Neutrophils." Scientific World JOURNAL 11 (2011): 173–85. http://dx.doi.org/10.1100/tsw.2011.25.

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Erythrocytes are known to shed vesiclesin vivo, under various conditionsin vitro, and, with impact for transfusion medicine, during storage of red blood cell concentrates (Vstovesicles). Vstovesicles of blood transfusions have been shown to deliver glycosylphosphatidylinositol-linked proteins to recipient erythrocytes, to display prothrombotic activity, and to have an inhibitory effect on macrophages. The interaction of Vstovesicles with and their effect on neutrophilic granulocytes has not yet been studied in detail. Fluorescentlylabeled Vstoand calcium-induced vesicles were preparedin order

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13

Schwab, Andreas J., Agnés Salamand, Yahye Merhi, André Simard, and Jocelyn Dupuis. "Kinetic analysis of pulmonary neutrophil retention in vivo using the multiple-indicator-dilution technique." Journal of Applied Physiology 95, no. 1 (2003): 279–91. http://dx.doi.org/10.1152/japplphysiol.00783.2001.

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Multiple-indicator-dilution experiments were performed in the lungs of 13 anesthetized dogs by simultaneous bolus injection of 111In-labeled neutrophils, 51Cr-labeled red blood cells, and Evans blue-labeled albumin. Concomitant counts of unlabeled neutrophils were similar in pulmonary artery and aortic blood samples, demonstrating a dynamic balance across the lungs in the physiological state. Outflow profiles of labeled neutrophils were analyzed on the basis of a recirculatory pharmacokinetic model of labeled albumin. The outflow profiles of the recovered neutrophils were composed of a through

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14

Kundu, SR Sarkar CK Yadav SK Sarker S. Hossain MG and Rauf SMA. "Pathological changes of liver and lung of slaughtered goats in Rajshahi Metropolitan area of Bangladesh." International Journal of Natural and Social Sciences 9, no. 1 (2022): 38–47. https://doi.org/10.5281/zenodo.6965400.

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A cross sectional study was carried out in the abattoirs of the Rajshahi Metropolitan area from where a total of randomly selected 131 samples (70 livers and 61 lungs) of goats were investigated by gross and microscopic examination. The purpose of the study was to determine the status vital organs e.g. liver and lungs of goat from public health perspective. Among 131 samples, 12 samples (9.16%) showed gross changes in liver and lungs. The changes in liver were bulging, flaccid and darkish discoloration with loose turbid capsule. It contained hemorrhage, congestion with rounded corners and nodu

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15

Yazidah, Maydina Izzatul, Didit Aspriyanto, and Renie Kumala Dewi. "EFFECTS OF RED DRAGON FRUIT PEEL EXTRACT ADMINISTRATION ON NEUTROPHIL COUNTS FOLLOWING PERIAPICAL RADIOGRAPHY EXPOSURE." Dentino : Jurnal Kedokteran Gigi 6, no. 2 (2021): 154. http://dx.doi.org/10.20527/dentino.v6i2.11998.

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Background: Radiation exposure from periapical imaging can cause cell damage in various tissues due to the formation of free radicals. Cell damage in the tissue can be observed from the number of neutrophils which are immune cells. Free radicals can be suppressed by administering exogenous antioxidants. One source of natural antioxidants is red dragon fruit, especially on its peel. Objective: To analyze the effect of periapical imaging x-ray radiation exposure after the administration of red dragon fruit peel extract on absolute neutrophil counts in mice. Method: This research was a true exper

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16

Brahmajothi, Mulugu, Stanely Mason, and Richard Auten. "S-nitrosothiols (SNO) imported through LAT1 (L-type amino acid transporter type 1) ameliorates phagocytosis in alveolar macrophages and neutrophils (MUC5P.868)." Journal of Immunology 192, no. 1_Supplement (2014): 134.11. http://dx.doi.org/10.4049/jimmunol.192.supp.134.11.

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Abstract Phagocytosis is a receptor mediated process where cytoskeletal rearrangement occurs to internalize an organism or particles. Macrophages and neutrophils have this potential as they form the first line of defense in any immune response. During lung inflammation, neutrophils and monocytes are freshly recruited; infiltrating neutrophils outnumber other cells but are short lived. As a result, more focus is on macrophages. Our study was the first to describe LAT1 expression in macrophages. We wanted to see the expression of LAT1 and its effects on SNO uptake in neutrophils. We administered

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17

Colletto, G. M. D. Dal, D. W. Fulker, O. C. de O. Barretto, and M. Kolya. "Genetic and Environmental Effects on Blood Cells." Acta geneticae medicae et gemellologiae: twin research 42, no. 3-4 (1993): 245–52. http://dx.doi.org/10.1017/s000156600000324x.

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AbstractIn a sample of 105 concordant sex MZ and DZ twin pairs, the following characteristics were measured: red cell count, haemoglobin concentration, package cell volume, mean cell volume, mean cell haemoglobin, mean cell haemoglobin concentration, reticulocytes, platelets, white cell count and the six types of leucocytes, lymphocytes, monocytes, band and segmented neutrophils, eosinophils and basophils. The statistical model employed in the univariate twin analysis allows for three sources of variation: genetic (h2), shared environmental (c2) and specific environmental influences (e2). A ge

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18

Tabata, Mika M., William M. Lin, Jay Goldstein, Timothy R. Quinn, and Steven T. Chen. "Localised chronic fibrosing vasculitis versus erythema elevatum diutinum." BMJ Case Reports 16, no. 1 (2023): e251515. http://dx.doi.org/10.1136/bcr-2022-251515.

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A woman in her 70s was referred for a painless plaque on the shin, present for 2 years and progressing in thickness. Examination revealed a large erythematous to violaceous indurated plaque with cobblestone appearance. Biopsy revealed an inflammatory infiltrate of neutrophils with scattered histiocytes, lymphocytes, eosinophils and plasma cells interspersed with areas of lamellar fibrosis and focal areas of vascular damage, suggestive of a localised chronic fibrosing vasculitis of the skin. Localised chronic fibrosing vasculitis is a rare dermatosis, typically presenting as ulcerated violet-re

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19

Weiss, D. J., and J. S. Klausner. "Neutrophil-induced Erythrocyte Injury: A Potential Cause of Erythrocyte Destruction in the Anemia Associated with Inflammatory Disease." Veterinary Pathology 25, no. 6 (1988): 450–55. http://dx.doi.org/10.1177/030098588802500608.

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Activated neutrophils at effector/target ratios of 1:20 to 1:2,000 induced depletion of red blood cell (RBC)-reduced glutathione, increased membrane-bound hemoglobin and methemoglobin, and promoted immunoglobulin binding to the cell membrane. Superoxide dismutase/catalase ameliorated the decrease in reduced glutathione and the increase in methemoglobin and immunoglobulin binding. This is interpreted as evidence that activated neutrophils can alter the antigenic nature of the RBC membrane through generation of toxic oxygen radicals.

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20

Arsenopoulos, Konstantinos V., Eleni Michalopoulou, Eleftherios Triantafyllou, George C. Fthenakis, and Elias Papadopoulos. "Hematological Parameters of Clinically Healthy Indigenous Greek Goats (Capra prisca) and Their Associations with Parasitological Findings, Age and Reproductive Stage." Agriculture 15, no. 13 (2025): 1445. https://doi.org/10.3390/agriculture15131445.

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Objectives: The present study aimed to determine the reference intervals for complete blood count and total protein parameters in Greek indigenous Capra prisca goats and to evaluate their associations with parasitic burden, age and reproductive stage. Methods: Two-hundred clinically health goats were grouped by parasite status (gastrointestinal nematodes, Eimeria spp., and lungworm infection), age (3–6-month-old growing kids; lactating non-pregnant goats ≤ 3 or >3 years old) and reproductive stage (non-lactating pregnant goats; lactating non-pregnant goats). Blood samples were analyzed for

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Sláma, Petr, Zbyšek Sládek, and Dušan Ryšánek. "Application of methods for detection of apoptosis and necrosis of bovine blood neutrophil granulocytes in vitro." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 54, no. 5 (2006): 107–16. http://dx.doi.org/10.11118/actaun200654050107.

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The study was an in vitro analysis of bovine blood neutrophil apoptosis and necrosis based on the detection of their morphological and biochemical features. The experiment was carried out in six clinically healthy Holstein x Bohemian Red Pied crossbred virgin heifers aged 16 to 18 months. The fresh and in vitro cultivated blood were analysed by light and electron microscopy and flow cytometry. In fresh blood, non apoptotic or necrotic neutrophils were found by light microscopy. On the other hand, 3.34% apoptotic and 0.32% necrotic neutrophils were detected by flow cytometry. After four hours o

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Goncharov, S. "The morphological changes in the blood of the predatory fish species with eustrongylidosis." Scientific Messenger of LNU of Veterinary Medicine and Biotechnology 21, no. 93 (2019): 15–20. http://dx.doi.org/10.32718/nvlvet9303.

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The article presents the results of the research concerning the morphological changes in the blood of the predatory fish species (perch, pike and zander) сaused by nematodical parasitic disease – eustrongylidosis. The research has been conducted in 2014–2018. It has been found that in the blood of perch with eustrongylidosis the hemoglobin and the red-blood-cell count decreased by 6.87 and 42.11 respectively, the white-blood-cell count increased by 8.95%; the basophils and pseudobasophils count – by 129.17%; the eosinophils and pseudoeosinophils count – by 131%; the number of band and segmente

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Shvydchenko, I. N., E. Yu Bykovskaya, and V. V. Golubtsov. "Effect of multistage isolation of neutrophils on their counts and viability." Medical Immunology (Russia) 27, no. 1 (2025): 107–18. https://doi.org/10.15789/1563-0625-eom-2948.

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Despite numerous separation methods of neutrophils from peripheral blood, isolation of sufficient quantities of high-purity viable cells for quantitative determination of neutrophil cytokines and their mRNA expression still remains an actual issue. The recommended multi-step purification methods significantly prolong the cell isolation process, potentially leading to cell activation or apoptosis and resulting in significant cell loss. Preliminary purification of neutrophils is the most critical stage in terms of time spent, and several additional manipulations with cells. To address this chall

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Tabatabaei-Zavareh, Nooshin, Tim Le Fevre, Andy Kokaji, et al. "Direct isolation of untouched human neutrophils from whole blood in as little as 25 minutes (TECH2P.870)." Journal of Immunology 192, no. 1_Supplement (2014): 135.1. http://dx.doi.org/10.4049/jimmunol.192.supp.135.1.

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Abstract Neutrophils are the first line of defense against microbial infections. They sense pathogen-associated molecular patterns through a wide range of receptors including toll-like receptors. Once activated, neutrophils eliminate infection via the phagocytosis of pathogens, releasing proinflammatory cytokines and antimicrobial peptides and producing reactive oxygen species (ROS). The procedures to enrich neutrophils from human whole blood involve at least two steps to remove red blood cells (RBCs) and unwanted leukocytes. For example, neutrophils may be separated from other leukocytes usin

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Scapini, Patrizia, and Marco A. Cassatella. "Location in the spleen dictates the function of murine neutrophils." Journal of Experimental Medicine 214, no. 5 (2017): 1207–9. http://dx.doi.org/10.1084/jem.20170655.

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In this issue of JEM, Deniset et al. (https://doi.org/10.1084/jem.20161621) provide new data that extend our knowledge on the mechanisms whereby Streptococcus pneumoniae is cleared by the spleen. The authors identify novel populations of murine splenic neutrophils that localize in the red pulp and the marginal zone. During the acute phases of S. pneumoniae infection, these populations of splenic neutrophils act in concert with specialized macrophage and B cell populations to provide very rapid innate immune protection.

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Meinderts, Sanne M., Gabriella Baker, Stan van Wijk, et al. "Neutrophils acquire antigen-presenting cell features after phagocytosis of IgG-opsonized erythrocytes." Blood Advances 3, no. 11 (2019): 1761–73. http://dx.doi.org/10.1182/bloodadvances.2018028753.

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Abstract Neutrophils are particularly well known for their antimicrobial function. Although historically they are regarded as strictly a phagocyte of the innate immune system, over time it has become clear that neutrophils are versatile cells with numerous functions including innate and adaptive immune regulation. We have previously described a role for human neutrophils in antibody-mediated red blood cell (RBC) clearance. Under homeostatic conditions, neutrophils do not take up RBCs. Yet, when RBCs are immunoglobulin G (IgG) opsonized, which can occur in alloimmunization or autoimmunization r

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Conran, Nicola, Carolina Lanaro, Fernanda G. Pereira, Tohru Ikuta, Sara T. O. Saad, and Fernando F. Costa. "Anti-Apoptotic Effect, Mediated by Elevated Intracellular Cyclic AMP Levels, in Neutrophils of Sickle Cell Disease Patients." Blood 106, no. 11 (2005): 2343. http://dx.doi.org/10.1182/blood.v106.11.2343.2343.

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Abstract Sickle cell disease (SCD) is now widely regarded as a chronic inflammatory condition, characterized by high base-line leukocyte counts, alterations in inflammatory cytokine levels, vascular endothelial injury and increased red and white cell adhesiveness. We hypothesized that this inflammatory state may be exacerbated by alterations in the apoptotic process in inflammatory cells, such as neutrophils. Neutrophils were isolated from the peripheral blood of healthy controls and individuals with SCD in steady state by separation over a ficoll-paque gradient. Following washing and lysis of

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Wiggs, B. R., D. English, W. M. Quinlan, N. A. Doyle, J. C. Hogg, and C. M. Doerschuk. "Contributions of capillary pathway size and neutrophil deformability to neutrophil transit through rabbit lungs." Journal of Applied Physiology 77, no. 1 (1994): 463–70. http://dx.doi.org/10.1152/jappl.1994.77.1.463.

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Neutrophil margination within the pulmonary capillary is due to a delay in their transit compared with that of red blood cells (RBC). This delay has been attributed to the large fraction of capillary segments that are narrower than spherical neutrophils and differences between the time required for deformation of neutrophils and that required for deformation of RBC. This study investigated the characteristics of neutrophil deformation in vivo and the perfusion patterns of segments within capillary pathways. Studies comparing the extraction of neutrophils with that of nondeformable microspheres

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Umei, T., K. Takeshige, and S. Minakami. "NADPH-binding component of the superoxide-generating oxidase in unstimulated neutrophils and the neutrophils from the patients with chronic granulomatous disease." Biochemical Journal 243, no. 2 (1987): 467–72. http://dx.doi.org/10.1042/bj2430467.

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The NADPH-binding component of the neutrophil superoxide-generating oxidase was studied in the particulate oxidase fractions obtained from the neutrophils of normal and chronic-granulomatous-disease (CGD) patients. The molecular mass of the NADPH-binding component of the stimulated human neutrophils, which was labelled with the 2′,3′-dialdehyde derivative of NADPH and sodium cyanoboro[3H]hydride, was 66 kDa. The 66 kDa component was also labelled in monocytes, but not in red blood cells, platelets and lymphocytes. The particulate oxidase fractions obtained from the patients with CGD had a dimi

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Bui, Y.-Nhi, Kimberly Boyle, Milena Batchvarova, Martha Delahunty, Marilyn J. Telen, and Grace M. Lee. "Red Cell Exchange Decreases Sickle RBC-Induced Release of MPO from Neutrophils." Blood 144, Supplement 1 (2024): 5277. https://doi.org/10.1182/blood-2024-207819.

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Introduction: Neutrophil activation has long been recognized to contribute to sickle cell disease (SCD) pathophysiology. In prior studies, we have shown that patients with SCD have activated neutrophils with enhanced degranulation responses as measured by matrix metalloproteinase-9 (MMP9) release, representative of tertiary granules. Furthermore, both simple transfusion and red cell exchange (RCE) decrease neutrophil activation and MMP9 release (Lee et al, Transfusion 2024), while exposure of normal neutrophils to sickle red cells (SS RBCs) promotes neutrophil activation, inducing both increas

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Simon, SI, YP Rochon, EB Lynam, CW Smith, DC Anderson, and LA Sklar. "Beta 2-integrin and L-selectin are obligatory receptors in neutrophil aggregation [see comments]." Blood 82, no. 4 (1993): 1097–106. http://dx.doi.org/10.1182/blood.v82.4.1097.1097.

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Abstract We have recently found that antibodies to L-selectin, the homing receptor on neutrophils, are as effective as those to beta 2-integrin at blocking formyl peptide-stimulated aggregation. Therefore, we investigated the requirements for expression of L-selectin and beta 2- integrin on adjacent cells during aggregation. Fluorescence flow cytometry allowed characterization of aggregates on the basis of size and color, as well as antibody binding to these two adhesive molecules. Formyl peptide-stimulated aggregate formation was measured for individual populations fluorescently labeled red (

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Simon, SI, YP Rochon, EB Lynam, CW Smith, DC Anderson, and LA Sklar. "Beta 2-integrin and L-selectin are obligatory receptors in neutrophil aggregation [see comments]." Blood 82, no. 4 (1993): 1097–106. http://dx.doi.org/10.1182/blood.v82.4.1097.bloodjournal8241097.

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We have recently found that antibodies to L-selectin, the homing receptor on neutrophils, are as effective as those to beta 2-integrin at blocking formyl peptide-stimulated aggregation. Therefore, we investigated the requirements for expression of L-selectin and beta 2- integrin on adjacent cells during aggregation. Fluorescence flow cytometry allowed characterization of aggregates on the basis of size and color, as well as antibody binding to these two adhesive molecules. Formyl peptide-stimulated aggregate formation was measured for individual populations fluorescently labeled red (LDS-751)

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Askarifirouzjaei, Hadi, Leila Khajoueinejad, Elena Wei, et al. "Sex Differences in Immune Cell Infiltration and Hematuria in SCI-Induced Hemorrhagic Cystitis." Pathophysiology 30, no. 3 (2023): 275–95. http://dx.doi.org/10.3390/pathophysiology30030023.

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Rats manifest a condition called hemorrhagic cystitis after spinal cord injury (SCI). The mechanism of this condition is unknown, but it is more severe in male rats than in female rats. We assessed the role of sex regarding hemorrhagic cystitis and pathological chronic changes in the bladder. We analyzed the urine of male and female Sprague-Dawley and Fischer 344 rats after experimental spinal cord contusion, including unstained microscopic inspections of the urine, differential white blood cell counts colored by the Wright stain, and total leukocyte counts using fluorescent nuclear stains. We

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Moreno-Carvalho, Otávio A., Maria-Regina A. Cardoso, and Cristiana M. Nascimento-Carvalho. "Neutrophils and red blood cells in the cerebrospinal fluid of newborns." Acta Paediatrica 97, no. 6 (2008): 816–18. http://dx.doi.org/10.1111/j.1651-2227.2008.00769.x.

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Chan, Diana Wen, Kristien J. Zaal, Evelyn Ralston, Sarthak Gupta, and Mariana J. Kaplan. "A novel real-time imaging technique to quantify mechanisms of cell death in neutrophils." Journal of Immunology 198, no. 1_Supplement (2017): 81.11. http://dx.doi.org/10.4049/jimmunol.198.supp.81.11.

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Abstract Neutrophils have distinct mechanisms of cell death, including the formation of neutrophil extracellular traps (NETs- a process called NETosis). NETosis is characterized by reactive oxygen species generation, chromatin decondensation, membrane rupture and extrusion of a meshwork of chromatin bound to granule peptides. Objective, efficient techniques to quantify NETosis are required. We developed a new method to automatically quantify the percentage of neutrophils undergoing NETosis using the IncuCyte ZOOM™ (Essen BioScience, Inc.) platform to image neutrophils at 20× in a 96-well plate

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Sae-Khow, Kritsanawan, Awirut Charoensappakit та Asada Leelahavanichkul. "Neutrophil Diversity (Immature, Aged, and Low-Density Neutrophils) and Functional Plasticity: Possible Impacts of Iron Overload in β-Thalassemia". International Journal of Molecular Sciences 25, № 19 (2024): 10651. http://dx.doi.org/10.3390/ijms251910651.

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Neutrophil dysfunction is a form of immune suppression in patients with β-thalassemia (Beta-thal), although data on this are limited. In this study, blood from patients and healthy volunteers was analyzed. Flow cytometry analysis demonstrated an increase in immature neutrophils (CD16− CD62L+) and aged (senescent) neutrophils (CD16+ CD62L−) in Beta-thal patients compared to healthy volunteers. The Beta-thal neutrophils demonstrated less prominent chemotaxis and phagocytosis than healthy neutrophils at the baseline. With phorbol myristate acetate (PMA) or lipopolysaccharide (LPS) stimulations, s

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Mitchell, Debra J., Pauline Li, Paul H. Reinhardt, and Paul Kubes. "Importance of L-selectin–dependent leukocyte–leukocyte interactions in human whole blood." Blood 95, no. 9 (2000): 2954–59. http://dx.doi.org/10.1182/blood.v95.9.2954.009k28_2954_2959.

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The objective of this study was to investigate whether leukocytes could be recruited by rolling leukocytes in a human whole blood model system. In all experiments, either neutrophils, whole blood, or diluted blood was perfused over immobilized E-selectin. With isolated neutrophils (2 × 105/mL), the free-flowing neutrophils were captured by attached neutrophils to form secondary interactions that resulted in lines of rolling leukocytes. These secondary tethers accounted for 50% to 60% of all interactions and were eliminated by an L-selectin antibody, which also eliminated the lines of rolling l

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Abadie, Valérie, Edgar Badell, Patrice Douillard, et al. "Neutrophils rapidly migrate via lymphatics after Mycobacterium bovis BCG intradermal vaccination and shuttle live bacilli to the draining lymph nodes." Blood 106, no. 5 (2005): 1843–50. http://dx.doi.org/10.1182/blood-2005-03-1281.

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Abstract The early innate response after Mycobacterium bovis bacille Calmette-Guérin (BCG) vaccination is poorly characterized but probably decisive for subsequent protective immunity against tuberculosis. Therefore, we vaccinated mice with fluorescent BCG strains in the ear dorsum, as a surrogate of intradermal vaccination in humans. During the first 3 days, we tracked BCG host cells migrating out of the dermis to the auricular draining lymph nodes (ADLNs). Resident skin dendritic cells (DCs) or macrophages did not play a predominant role in early BCG capture and transport to ADLNs. The main

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Pinheiro, Luís, Catarina Cipriano, Filipe Santos, et al. "Floridoside Phosphotriester Derivatives: Synthesis and Inhibition of Human Neutrophils’ Oxidative Burst." Molecules 30, no. 13 (2025): 2850. https://doi.org/10.3390/molecules30132850.

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Floridoside (2-O-D-glycerol-α-D-galactopyranoside) is a natural product typically found in red algae. It serves as the algae’s carbon reserve and is produced as a protective response against osmotic and heat stress. Both floridoside and its acylated derivatives have been associated with modulating redox homeostasis and inflammatory responses. Therefore, we aimed to evaluate whether the newly synthesized floridoside phosphotriesters (1b–1d, 1f–1h) and acylated floridoside derivative (1e) can modulate the oxidative burst in stimulated human neutrophils. Synthetic strategies included the glycosyl

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Cueva, Andrea, Patricio Freire, Verónica Posso, et al. "A Swollen Red Leg: Sweet’s Syndrome." Journal of Dermatology and Clinical Research 12, no. 1 (2024): 1–2. http://dx.doi.org/10.47739/dermatology.1160.

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Neutrophillic dermatoses comprise a range of conditions where the hallmark is histologic infiltrate of neutrophils. Ranging from skin disorders such as acute generalised exanthematous pustulosis or subcorneal pustulosis (Sneddon Wilkinson) which affect the epidermis, the neutrophilic infiltrate can affect the dermal portion such as in Neutrophillic urticaria, Sweet’s syndrome, or eccrine neutrophilic hidradenitis, or it can affect both epidermis and dermis such as in pyoderma gangrenosum. Many times the diagnosis of a neutrophilic dermatosis poses a challenge as the main differential are infec

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Meinderts, Sanne M., Per-Arne Oldenborg, Boukje M. Beuger, et al. "Human and murine splenic neutrophils are potent phagocytes of IgG-opsonized red blood cells." Blood Advances 1, no. 14 (2017): 875–86. http://dx.doi.org/10.1182/bloodadvances.2017004671.

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Key Points In steady state, where no IgGs against RBCs are present, macrophages are the primary phagocytes of RBCs. In conditions where RBCs are IgG-opsonized, neutrophils can have a major effect on RBC clearance.

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Dembele, Abdoul Karim, Patricia Hermand-Tournamille, Florence Missud, et al. "Persistence of Chronic Inflammation Despite Years of Transfusion Program in SCD Patients: Changing Red Blood Cells Is Not Sufficient to Treat Sickle Cell Disease." Blood 138, Supplement 1 (2021): 764. http://dx.doi.org/10.1182/blood-2021-147787.

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Abstract Sickle cell disease (SCD) is a severe hemoglobinopathy due to abnormal hemoglobin S (HbS). Although red blood cell dysfunction is at the core of the SCD pathophysiology, several studies have highlighted the important role of inflammatory cells like neutrophils. One of the most serious complications of SCD is cerebral vasculopathy (CV), due to the occlusion of one or more intracranial or cervical arteries. In 1998, the STOP study demonstrated that monthly blood transfusions could reduce the risk of stroke by 90% in children with CV. However, there is large heterogeneity in the evolutio

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Gherroucha, Dounia, Louiza Benhamza, and Mohamed Gharbi. "Prevalence of parasitic lesions in lungs and livers of cattle and sheep at Constantine’s slaughterhouse, Northeast Algeria." Revue d’élevage et de médecine vétérinaire des pays tropicaux 75, no. 1 (2022): 19–24. http://dx.doi.org/10.19182/remvt.36868.

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A cross-sectional survey was conducted at Constantine’s slaughterhouse in Northeast Algeria between February 2018 and February 2019. Livers and lungs of 2574 sheep and 1036 cattle were examined for the presence of lesions. The overall prevalence of lung lesions was 33% and 32% in sheep and cattle, respectively. The most frequent lesion in sheep lungs was red hepatization (19%) followed by pleurisy (12%). In cattle, the most frequent lung lesion was cystic echinococcosis (24%), followed by pleurisy (7%) and emphysema (5%). Lungworm infection (9%), atelectasis (0.2%) and suppurative bronchopneum

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Sláma, P., Z. Sládek, and D. Ryšánek. "Effect of Isolation Techniques on Viability of Bovine Blood Neutrophils." Acta Veterinaria Brno 75, no. 3 (2006): 343–53. http://dx.doi.org/10.2754/avb200675030343.

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The effect of selected isolation methods on the viability of neutrophil granulocytes (neutrophils) from the blood of healthy Holstein x Bohemian Red Pied crossbred heifers was evaluated. Two methods of neutrophil isolation were used: a) neutrophil isolation on the basis of hypotonic erythrocyte lysis (in two variants: after the erythrocyte lysis proper, the cells were centrifuged at either 200 g or 1000 g), and b) neutrophil isolation with FACS Lysing Solution as the lysing agent. The viability of the isolated neutrophils was evaluated on the basis of apoptosis and necrosis. The results obtain

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Davis, Andrew K., and Joseph R. Milanovich. "Lead-phase and red-stripe color morphs of red-backed salamanders Plethodon cinereus differ in hematological stress indices: A consequence of differential predation pressure?" Current Zoology 56, no. 2 (2010): 238–43. http://dx.doi.org/10.1093/czoolo/56.2.238.

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Abstract Throughout the animal kingdom there are species that have two or more phenotypic forms or ‘morphs’, and many of these are amphibians. In North America, the red-backed salamander Plethodon cinereus can have either a red dorsal stripe or no dorsal stripe (lead-phase form), and evidence to date indicates the lead-phase form incurs a greater number of attacks from predators. In a recent collection of 51 P. cinereus, blood smears of both color morphs (35 red-stripe, 16 lead-phase) were examined to obtain numbers of circulating leukocytes (via light microscopy), which can be used to indirec

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Essig, Fabian, Alexander M. Kollikowski, Mirko Pham, et al. "Immunohistological Analysis of Neutrophils and Neutrophil Extracellular Traps in Human Thrombemboli Causing Acute Ischemic Stroke." International Journal of Molecular Sciences 21, no. 19 (2020): 7387. http://dx.doi.org/10.3390/ijms21197387.

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Ischemic stroke caused by thromboembolic occlusion of large cerebral arteries, such as the internal carotid (ICA) and/or the middle cerebral artery (MCA), is treated by mechanical thrombectomy (MT). MT allows salvage of the vessel-occluding thrombemboli, which most frequently originate from the left atrium or the left ventricle of the heart or from sites of plaque rupture within large arteries above the heart. Clot composition may influence the efficacy of (intravenous) thrombolysis and MT, respectively. We analyzed 37 human thrombemboli obtained from acute ischemic stroke patients during MT w

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Bennewitz, Margaret, Mark Gladwin, and Prithu Sundd. "Role of neutrophils in pulmonary vaso-occlusion during sickle cell disease acute chest syndrome (CAM3P.200)." Journal of Immunology 192, no. 1_Supplement (2014): 114.1. http://dx.doi.org/10.4049/jimmunol.192.supp.114.1.

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Abstract Sickle cell disease (SCD) is a hematological disorder that leads to sickling of red blood cells within the microcirculation. The sickle red blood cells (sRBCs) are not only less deformable, but also express α4β1, ICAM-4, Sialyl Lewis X and glycolipids not expressed on non SCD-RBCs. These rigid sRBCs can get sequestered in the microcirculation to cause painful vaso-occlusive crisis (VOC) in SCD patients. In the lung, VOC can contribute to acute chest syndrome, which is a form of acute lung injury. Several factors including the pro-adhesive sRBCs, the large marginated pool of neutrophil

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Damianaki, Athena, Elias Stagakis, Semeli Mastrodemou, et al. "Increased Frequency of Paroxysmal Nocturmal Hemoglobinuria Type Cells in Patients with Chronic Idiopathic Neutropenia Correlates with the Severity of the Disease and the Presence of Skewed T-Cell Expansions." Blood 126, no. 23 (2015): 4778. http://dx.doi.org/10.1182/blood.v126.23.4778.4778.

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Abstract Introduction: Chronic idiopathic neutropenia (CIN) of adults is an acquired disorder of granulopoiesis characterized by an unexplained, prolonged reduction in the number of peripheral blood (PB) neutrophils. We have previously shown that CIN pathophysiology is related to T-cell activation and increased apoptosis of granulocytic progenitor cells. Notably, in our cohort of patients we exclude cases with antineutrophil antibody activity, i.e. autoimmune neutropenia cases. Aim of the study: Preliminary data have shown the presence of minor populations of paroxysmal nocturmal hemoglobinuri

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Berkow, RL, and RW Dodson. "Purification and functional evaluation of mature neutrophils from human bone marrow." Blood 68, no. 4 (1986): 853–60. http://dx.doi.org/10.1182/blood.v68.4.853.853.

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Abstract Human myeloid maturation proceeds within the bone marrow and results in a mature neutrophil that is released into the peripheral circulation. Previous reports have indicated that neutrophils from bone marrow demonstrate decreased adherence, impaired phagocytosis, and decreased nitroblue tetrazolium dye reduction when stimulated. Due to lack of a suitable method for isolating purified bone marrow neutrophils, these studies have been performed by microscopic techniques. We now report a method for isolating 1 X 10(8) neutrophils [bands plus polymorphonuclear leukocytes (PMNs)] from 10 mL

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Berkow, RL, and RW Dodson. "Purification and functional evaluation of mature neutrophils from human bone marrow." Blood 68, no. 4 (1986): 853–60. http://dx.doi.org/10.1182/blood.v68.4.853.bloodjournal684853.

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Human myeloid maturation proceeds within the bone marrow and results in a mature neutrophil that is released into the peripheral circulation. Previous reports have indicated that neutrophils from bone marrow demonstrate decreased adherence, impaired phagocytosis, and decreased nitroblue tetrazolium dye reduction when stimulated. Due to lack of a suitable method for isolating purified bone marrow neutrophils, these studies have been performed by microscopic techniques. We now report a method for isolating 1 X 10(8) neutrophils [bands plus polymorphonuclear leukocytes (PMNs)] from 10 mL of bone

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