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Artykuły w czasopismach na temat „Neutrophils”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Forlow, S. Bradley, Jill R. Schurr, Jay K. Kolls, Gregory J. Bagby, Paul O. Schwarzenberger, and Klaus Ley. "Increased granulopoiesis through interleukin-17 and granulocyte colony-stimulating factor in leukocyte adhesion molecule–deficient mice." Blood 98, no. 12 (2001): 3309–14. http://dx.doi.org/10.1182/blood.v98.12.3309.

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Abstract Many mutant mice deficient in leukocyte adhesion molecules display altered hematopoiesis and neutrophilia. This study investigated whether peripheral blood neutrophil concentrations in these mice are elevated as a result of accumulation of neutrophils in the circulation or altered hematopoiesis mediated by a disrupted regulatory feedback loop. Chimeric mice were generated by transplanting various ratios of CD18+/+ and CD18−/− unfractionated bone marrow cells into lethally irradiated wild-type mice, resulting in approximately 0%, 10%, 50%, 90%, or 100% CD18 null neutrophils in the bloo

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Gong, Henry H., Matthew J. Worley, Kyle A. Carver, Caleb J. Godin, and Jane C. Deng. "Deficient neutrophil responses early in influenza infection promote viral replication and pulmonary inflammation." PLOS Pathogens 21, no. 1 (2025): e1012449. https://doi.org/10.1371/journal.ppat.1012449.

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Neutrophils play key protective roles in influenza infections, yet excessive neutrophilic inflammation is a hallmark of acute lung injury during severe infections. Phenotypic heterogeneity is increasingly recognized in neutrophil populations; however, how functional variation in neutrophils between individuals determine the diverse outcomes of influenza remains unclear. To examine immunologic responses that may drive varying outcomes in influenza, we infected C57BL/6 (B6) and A/J mice with mouse-adapted influenza A virus A/PR/8/34 H1N1. A self-resolving dose in B6 mice was lethal in A/J mice,

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McGovern, Toby K., Michael Chen, Benoit Allard, Kjell Larsson, James G. Martin, and Mikael Adner. "Neutrophilic oxidative stress mediates organic dust-induced pulmonary inflammation and airway hyperresponsiveness." American Journal of Physiology-Lung Cellular and Molecular Physiology 310, no. 2 (2016): L155—L165. http://dx.doi.org/10.1152/ajplung.00172.2015.

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Airway exposure to organic dust (OD) from swine confinement facilities induces airway inflammation dominated by neutrophils and airway hyperresponsiveness (AHR). One important neutrophilic innate defense mechanism is the induction of oxidative stress. Therefore, we hypothesized that neutrophils exacerbate airway dysfunction following OD exposure by increasing oxidant burden. BALB/C mice were given intranasal challenges with OD or PBS (1/day for 3 days). Mice were untreated or treated with a neutrophil-depleting antibody, anti-Ly6G, or the antioxidant dimethylthiourea (DMTU) prior to OD exposur

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Mizgerd, J. P., B. B. Meek, G. J. Kutkoski, D. C. Bullard, A. L. Beaudet, and C. M. Doerschuk. "Selectins and neutrophil traffic: margination and Streptococcus pneumoniae-induced emigration in murine lungs." Journal of Experimental Medicine 184, no. 2 (1996): 639–45. http://dx.doi.org/10.1084/jem.184.2.639.

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The roles of selectins in the pulmonary margination and emigration of neutrophils were investigated by using mice genetically deficient in both E- and P-selectins (E/P mutants) and/or by intravenous injections of fucoidin (inhibiting both L- and P-selectins). E/P mutants were neutrophilic (14.7 +/- 4.9 x 10(6) vs. 0.8 +/- 0.1 x 10(6) neutrophils/ml). This neutrophilia was associated with increased margination of neutrophils within pulmonary capillaries (39.7 +/- 9.4 vs. 4.6 +/- 1.1 neutrophil profiles per 100 red blood cell profiles) but no change in margination within noncapillary pulmonary m

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Yamasaki, Akira, Ryota Okazaki, and Tomoya Harada. "Neutrophils and Asthma." Diagnostics 12, no. 5 (2022): 1175. http://dx.doi.org/10.3390/diagnostics12051175.

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Although eosinophilic inflammation is characteristic of asthma pathogenesis, neutrophilic inflammation is also marked, and eosinophils and neutrophils can coexist in some cases. Based on the proportion of sputum cell differentiation, asthma is classified into eosinophilic asthma, neutrophilic asthma, neutrophilic and eosinophilic asthma, and paucigranulocytic asthma. Classification by bronchoalveolar lavage is also performed. Eosinophilic asthma accounts for most severe asthma cases, but neutrophilic asthma or a mixture of the two types can also present a severe phenotype. Biomarkers for the d

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Weinmann, Pamela, Karin Scharffetter-Kochanek, S. Bradley Forlow, Thorsten Peters, and Barbara Walzog. "A role for apoptosis in the control of neutrophil homeostasis in the circulation: insights from CD18-deficient mice." Blood 101, no. 2 (2003): 739–46. http://dx.doi.org/10.1182/blood-2002-01-0239.

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The control of neutrophil turnover in the circulation is a key event in homeostasis and inflammation. Using CD18- deficient (CD18−/−) mice that show a 19-fold increase of blood neutrophil counts when compared with wild-type animals (CD18+/+), we found that apoptosis of peripheral neutrophils was significantly reduced from 27.4% in the wild-type to 4.8% inCD18−/− mice within 4 hours after isolation as measured by analysis of DNA content. This was confirmed by detecting CD16 expression, nuclear morphology, and internucleosomal DNA degradation. In contrast, no difference in apoptosis was observed

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Borges, Leandro, Tania Cristina Pithon-Curi, Rui Curi, and Elaine Hatanaka. "COVID-19 and Neutrophils: The Relationship between Hyperinflammation and Neutrophil Extracellular Traps." Mediators of Inflammation 2020 (December 2, 2020): 1–7. http://dx.doi.org/10.1155/2020/8829674.

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Coronavirus disease 2019 (COVID-19) is a virus-induced respiratory disease that may progress to acute respiratory distress syndrome (ARDS) and is triggered by immunopathological mechanisms that cause excessive inflammation and leukocyte dysfunction. Neutrophils play a critical function in the clearance of bacteria with specific mechanisms to combat viruses. The aim of this review is to highlight the current advances in the pathways of neutrophilic inflammation against viral infection over the past ten years, focusing on the production of neutrophil extracellular traps (NETs) and its impact on

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Wang, Guoshun, and Hang Pong Ng. "Myeloid CFTR Loss-of-function Causes Persistent Neutrophilic Inflammation in Cystic Fibrosis." Journal of Immunology 202, no. 1_Supplement (2019): 187.33. http://dx.doi.org/10.4049/jimmunol.202.supp.187.33.

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Abstract Persistent neutrophilic inflammation is a hallmark manifestation of cystic fibrosis (CF). However, the mechanism underlying this phenomenal clinical symptom remains incompletely understood. Here we report a pivotal role of CFTR in myeloid immune cells in control of neutrophilic inflammation. Myeloid CFTR-Knockout (Mye-Cftr−/−) mice and Wild-type (WT) mice were challenged peritoneally with zymosan at different doses. The lethal-dose challenge resulted in significantly higher mortality in Mye-Cftr−/− mice, indicating an intrinsic defect in host protection against inflammation in CF. The

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9

Inauen, W., D. N. Granger, C. J. Meininger, M. E. Schelling, H. J. Granger, and P. R. Kvietys. "Anoxia-reoxygenation-induced, neutrophil-mediated endothelial cell injury: role of elastase." American Journal of Physiology-Heart and Circulatory Physiology 259, no. 3 (1990): H925—H931. http://dx.doi.org/10.1152/ajpheart.1990.259.3.h925.

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The aim of this study was to assess the role of neutrophilic elastase in anoxia-reoxygenation-induced, neutrophil-mediated injury to microvascular endothelium. Cultured bovine microvascular endothelial cells were grown to confluence and labeled with 51Cr. The endothelial cells were exposed to a 30-min period of anoxia and subsequently reoxygenated. Endothelial cell injury, quantitated as 51Cr release and cell detachment, was determined 8 h after reoxygenation. Addition of neutrophils upon reoxygenation enhanced the anoxia-reoxygenation-induced increase in 51Cr release and cell detachment. The

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10

Tomar, Bhawna, Hans-Joachim Anders, Jyaysi Desai, and Shrikant R. Mulay. "Neutrophils and Neutrophil Extracellular Traps Drive Necroinflammation in COVID-19." Cells 9, no. 6 (2020): 1383. http://dx.doi.org/10.3390/cells9061383.

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The COVID-19 pandemic is progressing worldwide with an alarming death toll. There is an urgent need for novel therapeutic strategies to combat potentially fatal complications. Distinctive clinical features of severe COVID-19 include acute respiratory distress syndrome, neutrophilia, and cytokine storm, along with severe inflammatory response syndrome or sepsis. Here, we propose the putative role of enhanced neutrophil infiltration and the release of neutrophil extracellular traps, complement activation and vascular thrombosis during necroinflammation in COVID-19. Furthermore, we discuss how ne

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11

Gadjeva, Mihaela, Abirami Kugadas, Anastasia Petenkova, Jennifer Geddes-McAlister, Michael K. Mansour, and David Sykes. "Neutrophil maturation and their response to infectious pathogens are regulated by microbiota." Journal of Immunology 202, no. 1_Supplement (2019): 127.22. http://dx.doi.org/10.4049/jimmunol.202.supp.127.22.

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Abstract It has long been considered that a neutrophil’s response to various infectious challenges is innately pre-determined. Here, we provide data that demonstrates that neutrophil proteomes are modulated by the microbiota. We found that the proteomic signatures of mature neutrophils derived from germ free (GF) and specific pathogen free (SPF) mice were significantly different. In the absence of microbiota, mature neutrophils lacked GM-CSF-driven priming. To identify molecular pathways, we set-up an in vitro system where neutrophil progenitors were transduced with lenti-guides to knock-down

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12

Wang, Jun-Xia, and Peter Nigrovic. "CD177 participates in a novel mechanism for regulating neutrophil recruitment (P3093)." Journal of Immunology 190, no. 1_Supplement (2013): 43.9. http://dx.doi.org/10.4049/jimmunol.190.supp.43.9.

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Abstract Background: Neutrophils are the sine qua non of inflammatory arthritis, and contribute to pain, swelling, and tissue injury, rendering neutrophil migration to the joint a potential therapeutic target. We recently found that the murine neutrophil surface protein Ly6G modulates chemotaxis via interaction with β2 integrins. Whereas CD177 is a neutrophil-specific human protein in the same gene family, we sought to identify the role of CD177 in human neutrophil recruitment. Method: Flow cytometry was used to analyze the levels of cell surface proteins in circulating neutrophils and in neut

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13

Teske, Sabine, Andrea A. Bohn, Jean F. Regal, Joshua J. Neumiller, and B. Paige Lawrence. "Activation of the aryl hydrocarbon receptor increases pulmonary neutrophilia and diminishes host resistance to influenza A virus." American Journal of Physiology-Lung Cellular and Molecular Physiology 289, no. 1 (2005): L111—L124. http://dx.doi.org/10.1152/ajplung.00318.2004.

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Unlike their role in bacterial infection, less is known about the role of neutrophils during pulmonary viral infection. Exposure to pollutant 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD, dioxin) results in excess neutrophils in the lungs of mice infected with influenza A virus. TCDD is the most potent agonist for the aryl hydrocarbon receptor (AhR), and exposure to AhR ligands has been correlated with exacerbated inflammatory lung diseases. However, knowledge of the effects of AhR agonists on neutrophils is limited. Likewise, the factors regulating neutrophil responses during respiratory viral

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14

Grisham, M. B., J. Everse, and H. F. Janssen. "Endotoxemia and neutrophil activation in vivo." American Journal of Physiology-Heart and Circulatory Physiology 254, no. 5 (1988): H1017—H1022. http://dx.doi.org/10.1152/ajpheart.1988.254.5.h1017.

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There is a growing body of data to suggest that marginated granulocytes mediate much of the pulmonary damage observed during endotoxemia. The mechanism(s) by which endotoxemia initiates neutrophil margination and cytotoxicity remain either controversial or unknown. The objectives of this study were 1) to determine the temporal relationship between endotoxin-induced decreases in mean arterial pressure and circulating neutrophils, 2) to monitor neutrophil activation in vivo by measuring myeloperoxidase (MPO) activity in the plasma and lymph, and 3) to assess the interaction between endotoxin and

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15

Mariscalco, M. Michele, M. Hossein Tcharmtchi, and C. Wayne Smith. "P-Selectin Support of Neonatal Neutrophil Adherence Under Flow: Contribution of L-Selectin, LFA-1, and Ligand(s) for P-Selectin." Blood 91, no. 12 (1998): 4776–85. http://dx.doi.org/10.1182/blood.v91.12.4776.

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Abstract To further define the neonatal neutrophil's ability to localize to inflamed tissue compared with adult cells, we examined the neonatal neutrophil interactions with P-selectin monolayers under two conditions: (1) attachment under constant shear stress and flow and (2) detachment where cells were allowed to attach in the absence of shear stress and then shear stress is introduced and increased in step-wise increments. Cord blood and adult neutrophils had minimal interactions with unstimulated human umbilical vein endothelial cells (HUVECs) at a constant shear stress of 2 dynes/cm2. Ther

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Mariscalco, M. Michele, M. Hossein Tcharmtchi, and C. Wayne Smith. "P-Selectin Support of Neonatal Neutrophil Adherence Under Flow: Contribution of L-Selectin, LFA-1, and Ligand(s) for P-Selectin." Blood 91, no. 12 (1998): 4776–85. http://dx.doi.org/10.1182/blood.v91.12.4776.412k32_4776_4785.

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To further define the neonatal neutrophil's ability to localize to inflamed tissue compared with adult cells, we examined the neonatal neutrophil interactions with P-selectin monolayers under two conditions: (1) attachment under constant shear stress and flow and (2) detachment where cells were allowed to attach in the absence of shear stress and then shear stress is introduced and increased in step-wise increments. Cord blood and adult neutrophils had minimal interactions with unstimulated human umbilical vein endothelial cells (HUVECs) at a constant shear stress of 2 dynes/cm2. There was a m

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17

Cavallaro, Elena C., Kar-Kate Liang, Kevin D. Forsyth, and Dani-Louise Dixon. "Neutrophil polarization in the airways of infants with bronchiolitis." Journal of Immunology 198, no. 1_Supplement (2017): 55.30. http://dx.doi.org/10.4049/jimmunol.198.supp.55.30.

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Abstract Pulmonary neutrophilia is observed in pediatric patients with viral-induced bronchiolitis. Despite an alleviation in clinical symptoms, our findings suggest that airway neutrophil infiltration and activation does not appear to resolve by discharge in hospitalized infants. The recent identification of neutrophil plasticity and polarization into distinct phenotypic pro-inflammatory (N1) and immunosuppressive (N2) subsets provides an unexplored avenue for regulation of the neutrophilic inflammatory response in bronchiolitis. Admission nasopharyngeal aspirate (NPA) samples were assessed f

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Teddleton, Hannah G., Javier J. Garza, Scott P. Greiner, and Scott A. Bowdridge. "157 Effect of Sheep Breed on Neutrophil Chemotaxis toHaemonchus Contortus Larval Antigen." Journal of Animal Science 101, Supplement_1 (2023): 105. http://dx.doi.org/10.1093/jas/skad068.126.

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Abstract Parasite-resistant St. Croix sheep generate a potent neutrophilic response to larval stages of Haemonchus contortus. Protective neutrophil responses can be best characterized by increased neutrophil infiltration to the abomasum within the first 3 days of infection. These data indicate breed differences in neutrophil chemotaxis and response to H. contortus infection. The purpose of this study was to determine differences in neutrophil chemotaxis by H. contortus third-stage larval (L3) antigen (HcLA). Suffolk (SUF) and St. Croix (STC) neutrophils were isolated and applied to matrigel-co

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Nwakoby, Izuchukwu E., Krishna Reddy, Puja Patel, et al. "Fas-Mediated Apoptosis of Neutrophils in Sera of Patients with Infection." Infection and Immunity 69, no. 5 (2001): 3343–49. http://dx.doi.org/10.1128/iai.69.5.3343-3349.2001.

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ABSTRACT In the presence of infection, neutropenia is considered to be a marker of poor prognosis; conversely, neutrophilia may not be a determinant of a better prognosis. Since apoptotic neutrophils are compromised functionally, we evaluated the effect of infection on neutrophil apoptosis. The rate of apoptosis was greater for neutrophils isolated from patients with infection than for healthy controls.Escherichia coli did not directly modulate the rate of neutrophil apoptosis. However, sera from infected patients promoted (P < 0.001) neutrophil apoptosis. Interestingly, the sera of patient

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White, Mitchell R., Tesfaldet Tecle, Erika C. Crouch, and Kevan L. Hartshorn. "Impact of neutrophils on antiviral activity of human bronchoalveolar lavage fluid." American Journal of Physiology-Lung Cellular and Molecular Physiology 293, no. 5 (2007): L1293—L1299. http://dx.doi.org/10.1152/ajplung.00266.2007.

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Surfactant protein D (SP-D) and neutrophils participate in the early innate immune response to influenza A virus (IAV) infection. SP-D increases neutrophil uptake of IAV and modulates neutrophil respiratory burst responses to IAV; however, neutrophil proteases have been shown to degrade SP-D, and human neutrophil peptide defensins bind to SP-D and can cause precipitation of SP-D from bronchoalveolar lavage fluid (BALF). BALF has significant antiviral activity against IAV. We first added neutrophils to BALF during incubation with IAV. Addition of neutrophils to BALF caused significantly greater

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Machado, Isabel Daufenback, José Roberto Santin, Carine Cristiane Drewes, et al. "Alterations in the profile of blood neutrophil membrane receptors caused by in vivo adrenocorticotrophic hormone actions." American Journal of Physiology-Endocrinology and Metabolism 307, no. 9 (2014): E754—E763. http://dx.doi.org/10.1152/ajpendo.00227.2014.

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Elevated levels of adrenocorticotrophic hormone (ACTH) mobilize granulocytes from bone marrow into the blood, although these neutrophils are refractory to a full migratory response into inflamed tissues. Here, we investigated the dependence of glucocorticoid receptor activation and glucocorticoid-regulated protein annexin A1 (ANXA1) on ACTH-induced neutrophilia and the phenotype of blood neutrophil after ACTH injection, focusing on adhesion molecule expressions and locomotion properties. ACTH injection (5 μg ip, 4 h) induced neutrophilia in wild-type (WT) mice and did not alter the elevated nu

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Harvath, L., K. B. Yancey, and S. I. Katz. "Selective inhibition of human neutrophil chemotaxis to N-formyl-methionyl-leucyl-phenylalanine by sulfones." Journal of Immunology 137, no. 4 (1986): 1305–11. http://dx.doi.org/10.4049/jimmunol.137.4.1305.

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Abstract The therapeutic efficacy of the sulfones, dapsone, and sulfoxone in neutrophilic dermatoses may be related to the effects of these drugs on neutrophil function. Therefore we determined whether neutrophil chemotactic migration to various chemoattractants could be inhibited by sulfones in vitro. The chemotactic responses of human neutrophils from healthy donors were tested by using N-formyl-methionyl-leucyl-phenylalanine (F-met-leu-phe), purified human C5a, and leukocyte-derived chemotactic factor (LDCF). Therapeutic concentrations of sulfones selectively inhibited neutrophil chemotaxis

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Djimde, Moussa, Kassoum Kayentao, Japhet Kabalu Tshiongo, et al. "Variation in neutrophil levels and artemisinin-based combination therapy efficacy in West-Africa." Journal of Infection in Developing Countries 17, no. 09 (2023): 1337–45. http://dx.doi.org/10.3855/jidc.17089.

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Introduction: Polymorphonuclear neutrophils (PMN) are involved in pathogen clearance by phagocytosis. However, the role of PMNs in the efficacy of artemisinin-based combination therapy (ACT) is poorly understood. Methodology: In a prospective longitudinal in vivo study, neutrophil rates were compared with malaria carriage after treatment with different ACTs: Artemether - lumefantrine (AL), Artesunate - amodiaquine (ASAQ), Dihydroartemisinin - piperaquine (DP) or Pyronaridine artesunate (PA). The study cases were classified as having neutropenia, normal neutrophil levels or neutrophilia dependi

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Albahrani, Khuzama, Jumanah Alessa, Baraa Falemban, Mayyadah Abdullah Alkuwayti, and Jamal Hussen. "NETosis and Calcium influx in Dromedary Camel Neutrophils after in vitro Toll-like Receptor Stimulation." World's Veterinary Journal 13 (March 25, 2023): 214–21. http://dx.doi.org/10.54203/scil.2023.wvj23.

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Neutrophilic granulocytes are vital immune cells of the early response to pathogens. They contribute to the antimicrobial response through phagocytosis, production of reactive oxygen species, cytokine production, degranulation, and NET-formation. Neutrophil extracellular traps (NETs), also known as NETosis, are a critical antibacterial effector mechanism of cells of myeloid effector cells, including neutrophils and macrophages. Toll-like receptors (TLRs) are pattern recognition receptors (PRRs) that mediate pathogen sensing through the recognition of microbial structures known as pathogen-asso

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Yamamoto, Gaku, Mahiru Kawano, Michiko Bun, et al. "Abstract 5329: Ovarian cancer predisposes neutrophils to form neutrophil extracellular traps(NETs)." Cancer Research 84, no. 6_Supplement (2024): 5329. http://dx.doi.org/10.1158/1538-7445.am2024-5329.

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Abstract Objective: Neutrophil extracellular trap (NETs) are neutrophil-derived extracellular DNA released in response to inflammation. In contrast to their primary host-defensive role, NETs have been recently reported to promote cancer progression. The aim of this study is to investigate the role of NETs in ovarian cancer progression. Method: The clinical data of ovarian cancer patients who underwent primary surgery at Osaka University Medical Hospital were retrospectively reviewed (n=340). The relationship between neutrophilia, peritoneal dissemination and prognosis were analyzed. Prior to i

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Shelite, Thomas R., Nicole L. Mendell, Donald H. Bouyer, David Hughes Walker, and Lynn Soong. "The role of neutrophils during Orientia infection." Journal of Immunology 196, no. 1_Supplement (2016): 66.28. http://dx.doi.org/10.4049/jimmunol.196.supp.66.28.

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Abstract Scrub typhus is a seriously neglected disease with approximately one-third of the world’s population at risk of being infected with Orientia tsutsugamushi, and the occurrence of over one million scrub typhus cases annually illustrate its importance in global health. All scrub typhus case studies that report blood cell counts, describe neutrophilia during the course of infection. Patients with confirmed scrub typhus have significant increases in activated neutrophil proteins in serum, and the increase of neutrophil recruiting cytokines. We also observed neutrophilia as well as neutroph

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Brinkworth, Jessica F., Kathrine Van Etten, Priya Bhatt, et al. "Functional comparison of human and non-human primate neutrophil responses." Journal of Immunology 202, no. 1_Supplement (2019): 73.21. http://dx.doi.org/10.4049/jimmunol.202.supp.73.21.

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Abstract Humans and apes are neutrophilic, maintaining high levels (50–70% of circulating leukocytes) of this short-lived, highly reactive cell type (neutrophils), while most primates maintain significantly lower proportions(20–40% circulating leukocytes). Neutrophils are key limiters of bacterial and parasitic infection, amplifying inflammation, phagocytosing and presenting, and trapping invading foreign material, as well as committing bystander tissue damage in these efforts. The comparative neutrophilia of humans, therefore, suggests alterations in neutrophil activities such that higher pro

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Hilda, J. Nancy, Sulochana Das, Srikanth P. Tripathy, and Luke Elizabeth Hanna. "Role of neutrophils in tuberculosis: A bird's eye view." Innate Immunity 26, no. 4 (2019): 240–47. http://dx.doi.org/10.1177/1753425919881176.

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Neutrophils are innate immune cells implicated in the process of killing Mycobacterium tuberculosis early during infection. Once the mycobacteria enter the human system, neutrophils sense and engulf them. By secreting bactericidal enzymes and α-defensins like human neutrophil peptides loaded in their granule armory, neutrophils kill the pathogen. Peripheral blood neutrophils secrete a wide range of cytokines like IL-8, IL-1-β and IFN-γ in response to mycobacterial infection. Thus they signal and activate distant immune cells thereby informing them of prevailing infection. The activated monocyt

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Sandri, Silvana, Cristina Bichels Hebeda, Milena Fronza Broering, et al. "Role of Annexin A1 Secreted by Neutrophils in Melanoma Metastasis." Cells 12, no. 3 (2023): 425. http://dx.doi.org/10.3390/cells12030425.

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Annexin A1 (AnxA1) is highly secreted by neutrophils and binds to formyl peptide receptors (FPRs) to trigger anti-inflammatory effects and efferocytosis. AnxA1 is also expressed in the tumor microenvironment, being mainly attributed to cancer cells. As recruited neutrophils are player cells at the tumor sites, the role of neutrophil-derived AnxA1 in lung melanoma metastasis was investigated here. Melanoma cells and neutrophils expressing AnxA1 were detected in biopsies from primary melanoma patients, which also presented higher levels of serum AnxA1 and augmented neutrophil–lymphocyte ratio (N

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Kologrivova, E. N., R. I. Pleshko, O. V. Cheremisina, and M. A. Boldyshevskaya. "Hypersegmentation of neutrophil nuclei in peripheral blood of patients with localized and advanced cancer of the larynx and laryngopharynx." Medical Immunology (Russia) 25, no. 5 (2023): 1111–16. http://dx.doi.org/10.15789/1563-0625-hon-2715.

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Neutrophilic granulocytes have a wide spectrum of functional activity. In recent years, the functional significance of neutrophils in the development and course of malignant neoplasms has been discussed. It has been shown that neutrophilic granulocytes can play pro- or antitumor activity. The aim of the study was to assess the structural and functional features of neutrophils in patients with varying degrees of prevalence of cancer of the larynx and laryngopharynx. Forty-one patients (aged 35-67) with newly diagnosed cancer of the larynx and laryngopharynx were examined and divided into subgro

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Bohner, Ariel M., Manasi Gadkari, Michelle Makiya, et al. "In vivocell tracking reveals the pattern of neutrophil tissue distribution at baseline and in response to glucocorticoid treatment." Journal of Immunology 210, no. 1_Supplement (2023): 79.02. http://dx.doi.org/10.4049/jimmunol.210.supp.79.02.

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Abstract Neutrophils are the most abundant circulating immune cell in humans and primates, and they play a central role in inflammation and innate immunity. Glucocorticoids (GCs) are the cornerstone of anti-inflammatory and immunosuppressive therapies. While GCs are known to induce neutrophilia, little is known about the underlying kinetics or molecular mechanisms. To better define the kinetics of GC-induced neutrophilia, we studied healthy human volunteers and rhesus macaques, and found that the neutrophilia peaks within 4 hours of GC infusion and lasts beyond 72 hours. Flow cytometry, nuclea

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Slavinsky, A. A., L. M. Chuprinenko, V. S. Verevkina, and E. S. Sevostyanova. "Blood and cell infiltrate neutrophilic leucocytes As inflammation markers in chronic endometritis: A prospective non-randomised controlled trial." Kuban Scientific Medical Bulletin 28, no. 2 (2021): 59–72. http://dx.doi.org/10.25207/1608-6228-2021-28-2-59-72.

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Background. Inflammation declares itself with the presence of cellular tissue infiltrate, which composition reflects the inflammation type. Chronic inflammation is predominated by mononuclear cell infiltration with a certain amount of neutrophils, which role and significance are not fully understood to date.Objectives. Assessment of the infiltrated neutrophil count at various chronic endometritis severity and its dependency on the functional and metabolic activity in neutrophilic leucocytes in peripheral blood.Methods. This prospective non-randomised controlled trial estimated the CD45+ leucoc

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Panova, Veera, Mayuri Gogoi, Noe Rodriguez-Rodriguez, et al. "Group-2 innate lymphoid cell-dependent regulation of tissue neutrophil migration by alternatively activated macrophage-secreted Ear11." Mucosal Immunology 14, no. 1 (2020): 26–37. http://dx.doi.org/10.1038/s41385-020-0298-2.

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AbstractType-2 immunity is characterised by interleukin (IL)-4, IL-5 and IL-13, eosinophilia, mucus production, IgE, and alternatively activated macrophages (AAM). However, despite the lack of neutrophil chemoattractants such as CXCL1, neutrophils, a feature of type-1 immunity, are observed in type-2 responses. Consequently, alternative mechanisms must exist to ensure that neutrophils can contribute to type-2 immune reactions without escalation of deleterious inflammation. We now demonstrate that type-2 immune-associated neutrophil infiltration is regulated by the mouse RNase A homologue, eosi

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von Vietinghoff, Sibylle, Gisela Tunnemann, Claudia Eulenberg, et al. "NB1 mediates surface expression of the ANCA antigen proteinase 3 on human neutrophils." Blood 109, no. 10 (2007): 4487–93. http://dx.doi.org/10.1182/blood-2006-10-055327.

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Abstract Antineutrophil cytoplasmic antibodies (ANCAs) with specificity for proteinase 3 (PR3) are central to a form of ANCA-associated vasculitis. Membrane PR3 (mPR3) is expressed only on a subset of neutrophils. The aim of this study was to determine the mechanism of PR3 surface expression on human neutrophils. Neutrophils were isolated from patients and healthy controls, and hematopoietic stem cells from cord blood served as a model of neutrophil differentiation. Surface expression was analyzed by flow cytometry and confocal microscopy, and proteins were analyzed by Western blot experiments

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Gordy, Claire, Heather Pua, Gregory D. Sempowski, and You-Wen He. "Regulation of steady-state neutrophil homeostasis by macrophages." Blood 117, no. 2 (2011): 618–29. http://dx.doi.org/10.1182/blood-2010-01-265959.

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Abstract The timely clearance of apoptotic neutrophils from inflammation sites is an important function of macrophages; however, the role of macrophages in maintaining neutrophil homeostasis under steady-state conditions is less well understood. By conditionally deleting the antiapoptotic gene cellular FLICE-like inhibitory protein (C-FLIP) in myeloid cells, we have generated a novel mouse model deficient in marginal zone and bone marrow stromal macrophages. These mice develop severe neutrophilia, splenomegaly, extramedullary hematopoiesis, decreased body weight, and increased production of gr

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Cain, Derek W., Yoshihiro Ueda, Thomas Matt Holl, Pilar B. Snowden, Motonari Kondo, and Garnett Kelsoe. "A comparison of “steady-state” and “emergency” granulopoiesis: evidence of a single pathway for neutrophil production (87.5)." Journal of Immunology 182, no. 1_Supplement (2009): 87.5. http://dx.doi.org/10.4049/jimmunol.182.supp.87.5.

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Abstract Normally, neutrophil pools are maintained by steady-state granulopoiesis in bone marrow (BM). Inflammation, however, elicits neutrophilias that outstrip the capacity of steady-state granulopoiesis, and are thought to rely on a distinct program of accelerated production known as "emergency" granulopoiesis. In mice immunized with alum adjuvant, IL-1RI expression by radioresistant cells is required for both the mobilization of neutrophils from BM and increased proliferation by hematopoietic progenitors. Thus, an IL-1RI dependent trans-acting factor(s) mediates the accelerated neutrophil

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Teddleton, Hannah G., Scott P. Greiner, and Scott A. Bowdridge. "21 Ancylostoma caninum-derived neutrophil inhibitory factor impairs ovine neutrophil chemotaxis to Haemonchus contortus larval antigen in Suffolk but not St. Croix sheep." Journal of Animal Science 102, Supplement_1 (2024): 56–57. http://dx.doi.org/10.1093/jas/skae019.067.

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Abstract Parasite-resistant St. Croix sheep (STC) generate a potent neutrophilic response to larval stages of Haemonchus contortus (Hc) as demonstrated by abomasal neutrophil infiltration as early as 3 days after infection. This phenomenon is delayed in parasite-susceptible Suffolk sheep (SUF) potentially contributing to larval establishment. An excretory/secretory (E/S) product, common to many helminths, is neutrophil inhibitory factor (NIF) which negatively affects neutrophil migration and activity. Due to differences in neutrophil accumulation between STC and SUF sheep, we hypothesized that

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Lodge, Katharine M., Andrew S. Cowburn, Wei Li, and Alison M. Condliffe. "The Impact of Hypoxia on Neutrophil Degranulation and Consequences for the Host." International Journal of Molecular Sciences 21, no. 4 (2020): 1183. http://dx.doi.org/10.3390/ijms21041183.

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Neutrophils are key effector cells of innate immunity, rapidly recruited to defend the host against invading pathogens. Neutrophils may kill pathogens intracellularly, following phagocytosis, or extracellularly, by degranulation and the release of neutrophil extracellular traps; all of these microbicidal strategies require the deployment of cytotoxic proteins and proteases, packaged during neutrophil development within cytoplasmic granules. Neutrophils operate in infected and inflamed tissues, which can be profoundly hypoxic. Neutrophilic infiltration of hypoxic tissues characterises a myriad

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Terashi, Kenji, Mikio Oka, Shigehiro Ohdo, et al. "Close Association between Clearance of Recombinant Human Granulocyte Colony-Stimulating Factor (G-CSF) and G-CSF Receptor on Neutrophils in Cancer Patients." Antimicrobial Agents and Chemotherapy 43, no. 1 (1999): 21–24. http://dx.doi.org/10.1128/aac.43.1.21.

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ABSTRACT Recombinant human granulocyte colony-stimulating factor (rhG-CSF) is used to counter chemotherapy-induced neutropenia. Our previous study showed an inverse correlation between serum rhG-CSF levels and the number of circulating neutrophils in cancer patients (H. Takatani, H. Soda, M. Fukuda, M. Watanabe, A. Kinoshita, T. Nakamura, and M. Oka, Antimicrob. Agents Chemother. 40:988–991, 1996). The aim of this study was to clarify the relationship between rhG-CSF clearance and G-CSF receptors on circulating neutrophils. In five cancer patients receiving chemotherapy, a bolus dose of rhG-CS

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Obana, Rintaro, and Masaaki Tamagawa. "Evaluation of Neutrophil’s Rotation on Transport Process of Membrane Concentration and its Underwater Propulsion by CFD Analysis." CFD Letters 17, no. 11 (2025): 220–28. https://doi.org/10.37934/cfdl.17.11.220228.

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In the immunological systems, neutrophil propulsion is driven by the concentration gradient of cytokines. However, there is no quantitative relationship between concentration gradient and propulsion. In our previous study, assuming that neutrophil moves by the concentration Marangoni effect, the concentration gradient of cytokine on the neutrophil membrane was experimentally examined. The results showed that the cytokine concentration gradient on the neutrophil membrane changed periodically. It was suggested that neutrophils move while rotating under cytokine concentration gradient. However, o

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Lopez, CM, Sciullo MP Di, Casas FN Claveles, et al. "Therapeutic targets to reduce the contribution of pulmonary neutrophilic inflammation towards obesity-associated co-morbidities: a mini-review." Open Journal of Pharmaceutical Science and Research 1, no. 1 (2019): 123–33. https://doi.org/10.36811/ojpsr.2019.110006.

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Epidemiology and experimental models have shown a close link between adipose tissue inflammation, systemic inflammation and pulmonary neutrophilic inflammation, which predispose obese patients to pulmonary diseases, obesity-associated co-morbidities and cancer. Increased content and activation of neutrophils in the lung microvasculature, resulting from peripheral activation of neutrophils, and increased adhesion of neutrophils to the lung microvasculature are important factors explaining the increased susceptibility of obese patients towards respiratory diseases and loss of insulin sensitivity

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Hsu, Alan Y., Decheng Wang, Sheng Liu, et al. "Phenotypical microRNA screen reveals a noncanonical role of CDK2 in regulating neutrophil migration." Proceedings of the National Academy of Sciences 116, no. 37 (2019): 18561–70. http://dx.doi.org/10.1073/pnas.1905221116.

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Neutrophil migration is essential for inflammatory responses to kill pathogens; however, excessive neutrophilic inflammation also leads to tissue injury and adverse effects. To discover novel therapeutic targets that modulate neutrophil migration, we performed a neutrophil-specific microRNA (miRNA) overexpression screen in zebrafish and identified 8 miRNAs as potent suppressors of neutrophil migration. Among those,miR-199decreases neutrophil chemotaxis in zebrafish and human neutrophil-like cells. Intriguingly, in terminally differentiated neutrophils,miR-199alters the cell cycle-related pathw

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Song, Zhimin, Guangming Huang, Luana Chiquetto Paracatu, et al. "NADPH oxidase controls pulmonary neutrophil infiltration in the response to fungal cell walls by limiting LTB4." Blood 135, no. 12 (2020): 891–903. http://dx.doi.org/10.1182/blood.2019003525.

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Abstract Leukocyte reduced NADP (NADPH) oxidase plays a key role in host defense and immune regulation. Genetic defects in NADPH oxidase result in chronic granulomatous disease (CGD), characterized by recurrent bacterial and fungal infections and aberrant inflammation. Key drivers of hyperinflammation induced by fungal cell walls in CGD are still incompletely defined. In this study, we found that CGD (CYBB−) neutrophils produced higher amounts of leukotriene B4 (LTB4) in vitro after activation with zymosan or immune complexes, compared with wild-type (WT) neutrophils. This finding correlated w

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Kumar, Sachin, Juying Xu, Magdalena Chrzanowska-Wodnicka, and Marie-Dominique Filippi. "The Small Gtpase Rap1b Negatively Regulates Neutrophil Migration During Inflammation By Limiting Trans-Cellular Diapedesis." Blood 122, no. 21 (2013): 320. http://dx.doi.org/10.1182/blood.v122.21.320.320.

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Abstract Neutrophils are the first line of cellular defense against infecting microorganisms by moving rapidly toward sites of infection. Impaired neutrophil recruitment and functions can cause life-threatening infections, while excessive neutrophil tissue infiltration contributes to inflammatory disorders and tissue injury. A number of key positive regulators of neutrophil tissue infiltration have been identified. However, the mechanisms that protect from unwanted inflammation by negative regulation of neutrophil recruitment and functions are still unrecognized. Rap1b is an evolutionary conse

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Lira, S. A., P. Zalamea, J. N. Heinrich, et al. "Expression of the chemokine N51/KC in the thymus and epidermis of transgenic mice results in marked infiltration of a single class of inflammatory cells." Journal of Experimental Medicine 180, no. 6 (1994): 2039–48. http://dx.doi.org/10.1084/jem.180.6.2039.

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Transgenic mice expressing the chemokine N51/KC in thymus, skin, and tongue showed a marked infiltration of a single class of inflammatory cells (neutrophils) in the sites of transgene expression. In the thymus, neutrophils were most numerous in the cortex and juxta-medullary regions, often forming aggregates or clusters. A similar, but less intense, neutrophilic infiltrate occurred in close proximity to the epidermal basal layer of the tongue and skin. No morphologic evidence of injury was observed in the thymus, skin, or tongue of these transgenic mice, indicating that N51/KC expression indu

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Kast, Richard E. "Research Supporting a Pilot Study of Metronomic Dapsone during Glioblastoma Chemoirradiation." Medical Sciences 9, no. 1 (2021): 12. http://dx.doi.org/10.3390/medsci9010012.

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This short note presents previous research data supporting a pilot study of metronomic dapsone during the entire course of glioblastoma treatment. The reviewed data indicate that neutrophils are an integral part of human glioblastoma pathophysiology, contributing to or facilitating glioblastoma growth and treatment resistance. Neutrophils collect within glioblastoma by chemotaxis along several chemokine/cytokine gradients, prominently among which is interleukin-8. Old data from dermatology research has shown that the old and inexpensive generic drug dapsone inhibits neutrophils’ chemotaxis alo

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Badve, Sunil, Andrea Blumstein, Peter Wiernik, and Howard Ratech. "Non-Hodgkin Malignant Lymphoma With Tissue Neutrophilia." Archives of Pathology & Laboratory Medicine 124, no. 5 (2000): 735–38. http://dx.doi.org/10.5858/2000-124-0735-nhmlwt.

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Abstract Context.—Neutrophils, in the absence of necrosis, are uncommon in non-Hodgkin malignant lymphoma. Recently, a neutrophil-rich type of Ki-1 (CD30)-positive, anaplastic large cell lymphoma was described. Objectives.—We report 3 cases of nonanaplastic large cell lymphoma with an abundance of tissue neutrophils; 2 cases were associated with breast carcinoma and possible infection. Results.—Peripheral blood neutrophilia was noted in only 1 of these 3 patients. Neutrophilia in the lymph nodes occurred in either a sinusoidal or interstitial pattern. Multiple biopsies were available for revie

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Zheng, Leyu, Moujie Rang, Carolin Fuchs, et al. "The Posttraumatic Increase in the Adhesion of GPCR EMR2/ADGRE2 to Circulating Neutrophils Is Not Related to Injury Severity." Cells 12, no. 22 (2023): 2657. http://dx.doi.org/10.3390/cells12222657.

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Trauma triggers a rapid innate immune response to aid the clearance of damaged/necrotic cells and their released damage-associated molecular pattern (DAMP). Here, we monitored the expression of EMR2/ADGRE2, involved in the functional regulation of innate immune cells, on circulating neutrophils in very severely and moderately/severely injured patients up to 240 h after trauma. Notably, neutrophilic EMR2 showed a uniform, injury severity- and type of injury-independent posttraumatic course in all patients. The percentage of EMR2+ neutrophils and their EMR2 level increased and peaked 48 h after

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Sládek, Z., and D. Ryšánek. "Apoptosis of neutrophilic granulocytes of bovine virgin mammary gland in scanning electron microscopy." Veterinární Medicína 46, No. 7–8 (2001): 185–89. http://dx.doi.org/10.17221/7881-vetmed.

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The objective of this work was the morphologic analysis of apoptosis of neutrophilic granulocytes (hereinafter referred to as neutrophils) in scanning electron microscopy (SEM) in comparison with morphological features distinguishable by light microscopy. This study was performed on 12 bovine virgin mammary glands washed with physiological buffered solution (PBS) prior to the induction of cell influx by PBS. Twenty-four hours after influx induction the cell suspension was obtained by the lavage of mammary glands with PBS. The particular lavages were cytologicaly and bacteriologicaly examined.

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Mittmann, Laura A., Florian Haring, Johanna B. Schaubächer, et al. "Uncoupled biological and chronological aging of neutrophils in cancer promotes tumor progression." Journal for ImmunoTherapy of Cancer 9, no. 12 (2021): e003495. http://dx.doi.org/10.1136/jitc-2021-003495.

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BackgroundBeyond their fundamental role in homeostasis and host defense, neutrophilic granulocytes (neutrophils) are increasingly recognized to contribute to the pathogenesis of malignant tumors. Recently, aging of mature neutrophils in the systemic circulation has been identified to be critical for these immune cells to properly unfold their homeostatic and anti-infectious functional properties. The role of neutrophil aging in cancer remains largely obscure.MethodsEmploying advanced in vivo microscopy techniques in different animal models of cancer as well as utilizing pulse-labeling and cell

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