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Rozprawy doktorskie na temat „Paper-based diagnostics”

Autor: Grafiati

Data publikacji: 6 września 2023

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1

Miller, Eric Alexander. "Development of thermostable affinity reagents for low-cost, paper-based medical diagnostics." Thesis, Massachusetts Institute of Technology, 2019. https://hdl.handle.net/1721.1/122849.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Chemical Engineering, 2019<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references.<br>The timely diagnosis and treatment of disease in resource-constrained settings requires the development of robust point-of-care (POC) diagnostics, which provide accurate results and can be employed by users with minimal medical training and limited access to basic infrastructure. One of the most common POC diagnostic formats is the immunochromatographic rapid diagnostic test, which traditionally uses nitrocellulose-i

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Ward, Jennifer Guerin. "Nitrocellulose Paper Based Microfluidic Platform Development and Surface Functionalization with Anti-IgE Aptamers." DigitalCommons@CalPoly, 2012. https://digitalcommons.calpoly.edu/theses/746.

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The purpose of this thesis project was to demonstrate the ability to utilize the capabilities of aptamers so that they may act as capture reagents for paper microfluidic devices. Several characterization experiments were conducted as a precursor before the final experimentation was performed. Paper characterization, manufacturing protocols for printing and heating, as well as 3D chip fabrication were all performed and analyzed. The results confirmed that the control of fluid through a 3D microfluidic device based in nitrocellulose is possible. For the biochemistry portion of this thesis rep

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Lama, Lara. "Novel methods for improving rapid paper-based protein assays with gold nanoparticle detection." Licentiate thesis, KTH, Proteomik och nanobioteknologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-214065.

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This thesis describes methods for improving sensitivity in rapid singleplex and multiplex microarray assays. The assays utilize the optical characteristics of colloidal gold nanoparticles for the colorimetric detection of proteins. Multiplexed detection in sandwich immunoassays is limited by cross-reactivity between different detection antibodies. The cross-reactivity between antibodies can contribute to increased background noise - decreasing the Limit-of-Detection of the assay - or generate false positive signals. Paper I shows improved assay sensitivity in a multiplexed vertical flow assay

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Glavan, Ana. "Chemical Approaches to the Surface Engineering of Paper and Cellulose-Based Materials for Microfluidics, Electronics and Low-Cost Diagnostics." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:26718749.

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Paper (and other cellulose-based materials such as cotton thread and fabrics) are underexploited as materials for the construction of “high-tech” and “lab-on-a-chip” devices. One major drawback of paper is its tendency to absorb water from the environment and, with wetting, to change its mechanical properties; other challenges relate to control over the attachment of molecules (e.g. antibodies, DNA) and cells on its surface, and to the addition of electronic function. The goal of this thesis is to develop paper as a substrate for a range of applications— microfluidics, substrates for electroni

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Katis, Ioannis. "Laser direct write techniques for the fabrication of paper-based diagnostic devices." Thesis, University of Southampton, 2015. https://eprints.soton.ac.uk/388397/.

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We report on the use of laser direct-write techniques for the fabrication of point-of-care paper-based diagnostic sensors. These include laser-based deposition, laser ablation and laser-induced photo-polymerisation. Firstly, Laser Induced Forward Transfer (LIFT) was employed to deposit biomolecules from a donor film onto paper receivers. Paper was chosen as the ideal receiver because of its inherent properties which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged antibodies were LIFT-printed and their viability was confirmed via

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Tageson, Mackenzie Elizabeth. "FUNCTIONAL 3-D CELLULOSE & NITROCELLULOSE PAPER-BASED, MULITPLEX DIAGNOSTIC PLATFORMS WITHOUT COUPLING AGENTS." DigitalCommons@CalPoly, 2013. https://digitalcommons.calpoly.edu/theses/1128.

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The purpose of this thesis was to demonstrate device functionality of 3-D paper-based, multiplex platforms, µPADs, without the use of coupling agents between layers. Previously, these platforms were fabricated with double-sided tape and cellulose powder to try to augment proper fluid routing, but difficulties with this method occurred. An acrylic housing unit with strategically placed pressure tabs was designed to aid horizontal and vertical fluid routing through the platform, thus eliminating the inconsistencies associated with coupling agents. Channel characterization studies, a COMSOLTM sim

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Ben, Aissa Soler Alejandra. "Rapid diagnostic test for the detection of communicable diseases." Doctoral thesis, TDX (Tesis Doctorals en Xarxa), 2020. http://hdl.handle.net/10803/670392.

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La prevenció i el control de les malalties transmissibles depenen, en gran mesura, de la detecció ràpida i eficaç. Els mètodes convencionals per a la detecció d'un patogen, com ara el cultiu microbiològic, generalment requereixen molt de temps, són laboriosos, necessiten personal qualificat i no són aptes com a eines de diagnòstic en el punt d'atenció. El desenvolupament de mètodes de diagnòstic ràpid en el marc dels criteris ASSURED, de l'anglès (A) Affordable, (SS) Sensitive i Didàctiques, (O) User-friendly, (R) Rapid and Robust, (I) Equipment free, and (d) Deliverable to those who need i

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Mitchell, Haydn Thomas. "AN INVESTIGATION OF POLY(N-ISOPROPYLACRYLAMIDE) FOR APPLICATIONS WITH MICROFLUIDIC PAPER-BASED ANALYTICAL DEVICES." DigitalCommons@CalPoly, 2014. https://digitalcommons.calpoly.edu/theses/1248.

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N,N′-methylenebisacrylamide-crosslinked poly(N-isopropylacrylamide), also known as P(NIPAM), was developed as a fluid delivery system for use with microfluidic paper-based analytical devices (microPADs). MicroPADs are postage-stamp-sized devices made out of paper that can be used as platforms for low-cost, simple-to-use point-of-care diagnostic assays. P(NIPAM) is a thermally responsive polymer that absorbs aqueous solutions at room temperature and will expel the solutions to microPADs when heated. The fluid delivery characteristics of P(NIPAM) were assessed, and P(NIPAM) was able to deliver m

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Morbioli, Giorgio Gianini. "Funcionalização de celulose para ensaios bioanalíticos em dispositivos microfluídicos baseados em papel (μPADs)." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/75/75135/tde-23062015-105938/.

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A funcionalização da matriz celulósica é um ponto essencial para o aprimoramento dos dispositivos microfluídicos baseados em papel (µPADs). Ela permite minimizar o preparo de amostras e a interferência do usuário, principais fontes de erro no processo analítico. A oxidação da celulose durante uma hora com m-periodato de sódio e a imobilização química de enzimas a partir da formação de bases de Schiff (iminas), via a adição direta da enzima ao substrato oxidado sem a necessidade de outras etapas, é um processo rápido e de baixo custo, apresentando grande potencialidade de aplicação nos di

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Braff, Dana. "Technological advancements towards paper-based biomolecular diagnostics." Thesis, 2017. https://hdl.handle.net/2144/27009.

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Clinically tractable diagnostics must be low-cost, rapid, sensitive, easy to use, and adaptable to new targets. With its rational design, synthetic biology holds promise for developing diagnostic technologies that can address these needs. In particular, progress in synthetic biology has led to improved circuit-building abilities and a large collection of biomolecular sensors. However, these technologies fundamentally require transcription and translation, limiting their applicability to cellular contexts In vitro cell-free expression systems that contain transcription and translation machiner

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"Microfluidic Paper-Based Analytical Devices: Sample Preparation, Medical Monitoring and Diagnostics." 2013.

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Rodriguez, Natalia Maria. "A paper-based point-of-care molecular diagnostic platform for the developing world." Thesis, 2016. https://hdl.handle.net/2144/14636.

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The disproportionate burden of infectious disease and lack of appropriate diagnostic tools in the developing world suggest that future health technology development efforts need to more effectively target these resource-limited settings. Microfluidic systems, like lab-on-a-chip technologies, offer the potential to miniaturize the large, complex processes performed in first-world laboratories onto a portable chip for use in remote settings. The problem with these systems is that they require equipment for fluidic handling and many other aspects of diagnostic assays such as sample preparation a

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(11192533), Kaleb M. Byers. "Platforms and Molecular Mechanisms for Improving Signal Transduction and Signal Enhancement in Multi-step Point-Of-Care Diagnostics." Thesis, 2021.

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<p>Swift recognition of disease-causing pathogens at the point-of-care enables life-saving treatment and infection control. However, current rapid diagnostic devices often fail to detect the low concentrations of pathogens present in the early stages of infection, causing delayed and even incorrect treatments. Rapid diagnostics that require multiple steps and/or elevated temperatures to perform have a number of barriers to use at the point-of-care and in the field, and despite efforts to simplify these platforms for ease of use, many still require diagnostic-specific training for the healthcar

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Kaur, Navjot. "FLIPP-NAAT: A paper-based LAMP assay for point-of-care TB detection." Thesis, 2021. https://etd.iisc.ac.in/handle/2005/5232.

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Molecular diagnostic techniques like polymerase chain reaction (PCR) revolutionized the world of healthcare. Instead of waiting for infectious agents to physically grow as in conventional culture-based tests, genetic material of pathogens could be used as a biomarker to detect their presence. Though PCR has deeply penetrated and greatly benefited the field of disease diagnosis, infrastructure required to perform PCR testing makes it unaffordable and hence inaccessible for majority of world population. The COVID-19 pandemic has strongly highlighted many such grave limitations of existing molecu

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(10725807), Jiangshan Wang. "A RAPID PAPER-BASED COLORIMETRIC MOLECULAR TEST FOR SARS-COV-2 POINT-OF-CARE DIAGNOSTIC." Thesis, 2021.

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<p>In the year of 2020, an international pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has afflicted tens of millions of people’s life also disrupting global economics. Diagnostic testing is an important part of ensuring public health until a vaccine that has been shown to be safe and effective is made available to the general public. Most tests for detecting COVID-19 utilize quantitative polymerase chain reaction (qPCR) assays, which is a specific and relatively simple quantitative assay that could provide adequate sensitivity for diagnosing early infection.

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Rijo, Catarina Filipa Braz. "A paper-based low-cost label-free biosensing silver core-gold shell nanostructure for SERS to be applied to breast cancer diagnostics." Master's thesis, 2019. http://hdl.handle.net/10362/91955.

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Breast cancer (BCa) is one of the most common and deadly diseases in women worldwide. In 2018, 2.1 million cases were diagnosed with BCa from which 626,679 women died. Therefore, an early diagnosis is imperative for treatment and a cure success rate. Recent studies have concluded that exosomes can be used as biomarkers, since they participate in the communication between cells, carrying genetic information from the mother cells. Common methods of detection of exosomes as Enzyme-Linked Immunosorbent Assays (ELISA) are used, however, large amounts of highly concentrated sample, special preparat

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Wu, Po-Chen, and 吳柏辰. "Paper-Based Diagnostic System." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/v3f4j8.

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碩士<br>國立臺灣大學<br>化學工程學研究所<br>101<br>Paper-based sensors are rapidly immerging to meet the needs for point-of-care diagnose. Due to its low cost, portable, widely available and easy to operate properties, it has attracted lots of attention in biochemical analysis field. In this work, paper 96 well plate was fabricated via wax printing method to build up a multiple diagnostic system. horseradish peroxidase (HRP enzyme) was conjugated with monoclonal antibody to vascular endothelial grow factor (VEGF antibody), and the quantification of the enzyme was carried out on paper 96 well plate. The influe

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Shen, Shu-Wei, and 沈書維. "Colorimetric-Based Tuberculosis?Diagnosis Using Gold Nanoparticles?on Paper-Based Diagnostic Devices." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/99565950911230803082.

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碩士<br>國立中興大學<br>生醫工程研究所<br>102<br>Tuberculosis has always been considered one of the most serious infectious disease, with the advancement of science and medical technology, people continuously find new ways of diagnosing tuberculosis from old theories, but still unable to develop a real-time, convenient and effective diagnostics. In this study, a colorimetric sensing strategy employing gold nanoparticles and a paper assay platform has been developed for tuberculosis diagnosis. Unmodified gold nanoparticles and single-stranded detection oligonucleotides are used to achieve rapid diagnosis, thr

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Fernandes, Alexandra Agostinho Gomes. "An electrochromic paper-based device as a diagnostic test for Cystic Fibrosis." Master's thesis, 2018. http://hdl.handle.net/10362/58114.

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Cystic Fibrosis (CF) is an inherited disorder affecting more than 70000 people worldwide, especially Caucasian populations with a carrier prevalence of 1/3000. Currently, it has no cure but an early diagnosis remains a critical issue. Sweat chloride test has been the gold standard to diagnose CF since the affected present sweat chloride concentrations ≥ 60 mM. In this work, a planar electrochromic “point-of-care” device, based on tungsten trioxide nanoparticles produced by microwave assisted hydrothermal synthesis, was developed as a first approach for CF diagnostic testing especially in resou

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"RNA-Based Computing Devices for Intracellular and Diagnostic Applications." Doctoral diss., 2019. http://hdl.handle.net/2286/R.I.54869.

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abstract: The fundamental building blocks for constructing complex synthetic gene networks are effective biological parts with wide dynamic range, low crosstalk, and modularity. RNA-based components are promising sources of such parts since they can provide regulation at the level of transcription and translation and their predictable base pairing properties enable large libraries to be generated through in silico design. This dissertation studies two different approaches for initiating interactions between RNA molecules to implement RNA-based components that achieve translational regulation.

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"Thermal Actuation and Fluidic Characterization of a Fluorescence-Based Multiplexed Detection System." Master's thesis, 2018. http://hdl.handle.net/2286/R.I.50454.

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abstract: This work describes efforts made toward the development of a compact, quantitative fluorescence-based multiplexed detection platform for point-of-care diagnostics. This includes the development of a microfluidic delivery and actuation system for multistep detection assays. Early detection of infectious diseases requires high sensitivity dependent on the precise actuation of fluids. Methods of fluid actuation were explored to allow delayed delivery of fluidic reagents in multistep detection lateral flow assays (LFAs). Certain hydrophobic materials such as wax were successfully imple

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