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Artykuły w czasopismach na temat „PAS Stain”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Hirota, Shigeru, Minoru Nomoto, Sadahiro Hosobe, Yutaka Aoyagi, and Hitoshi Asakura. "Mucin in Primary Liver Carcinomas: Combined Hepatocellular-Cholangiocarcinoma or Variant Hepatocellular Carcinoma." Canadian Journal of Gastroenterology 10, no. 1 (1996): 12–16. http://dx.doi.org/10.1155/1996/808592.

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OBJECTIVE: To investigate whether the presence of mucin defines a combined hepatocellular-cholangiocarcinoma or merely a variant of usual hepatocellular carcinoma (HCC).METHODS: From 1979-92, 124 cases of usual HCC were studied at Niigata University Hospital, Niigata City, Japan, and several affiliated hospitals. Histological diagnoses were determined according to World Health Organization (WHO) criteria. Hematoxylin and eosin stain, periodic acid-Schiff (PAS) stain, PAS stain after diastase digestion (D-PAS) and silver stain tests were performed. Cases containing D-PAS-positive substances wer

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Alkhamiss, Abdullah Saleh. "Evaluation of Better Staining Method among Hematoxylin and Eosin, Giemsa and Periodic Acid Schiff-Alcian Blue for the Detection of Helicobacter pylori in Gastric Biopsies." Malaysian Journal of Medical Sciences 27, no. 5 (2020): 53–61. http://dx.doi.org/10.21315/mjms2020.27.5.6.

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Background: This study was undertaken to evaluate the preferred method (Giemsa or periodic acid Schiff-Alcian blue [PAS-AB] stains) of detecting Helicobacter pylori (H. pylori) in gastric mucosal biopsies in terms of sensitivity, specificity and applicability. To the best of my knowledge, this is the first report comparing Giemsa and PAS-AB staining for the detection of H. pylori in such biopsies. Methods: The formalin-fixed paraffin-embedded blocks of 49 gastric biopsies from different patients were collected from the archive of anatomical pathology at King Abdulaziz Medical City, National Gu

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Ghritlahare, Himanta, A. Einstein, Swatantra Patel, Rashmi C. Kerketta, Shubhangi D. Mishra, and Shirish K. Kujur. "Cost-Effective Staining Alternatives to Immunohistochemistry for Keratin Demonstration in Oral Epithelial Pathologies." Journal of Pharmacy and Bioallied Sciences 16, Suppl 3 (2024): S2827—S2829. http://dx.doi.org/10.4103/jpbs.jpbs_313_24.

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ABSTRACT Background: Demonstrating keratin is crucial in diagnosing various epithelial pathologies and is typically done through histological and immunohistochemical (IHC) methods. While IHC staining for keratin is highly specific, it can be costly and time-consuming. Therefore, common histological staining methods for keratin, suitable for routine histotechniques labs, are gaining importance. Methodology: Twenty formalin-fixed, paraffin-embedded tissue blocks, each representing histologically confirmed normal oral mucosa (NOM), hyperorthokeratosis (HOK), and well-differentiated squamous cell

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4

Belkacem, Oussama. "USE OF ROUTINE SPECIAL STAINS FOR GASTRIC AND ESOPHAGEAL BIOPSIES." EPH - International Journal of Medical and Health Science 4, no. 1 (2018): 80–84. http://dx.doi.org/10.53555/eijmhs.v4i1.29.

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Problem: Special stains such as the Giemsa, the Alcian blue (AB) and the Periodic Acid Schiff (PAS) are widely used for gastric and/or esophageal biopsies for the diagnosis of Helicobacter pylori (H. pylori) and intestinal metaplasia (IM). The purpose of our study was to determine if these stains are actually needful Methods: We retrospectively studied 209 gastric and esophageal biopsies. We evaluated the H. pylori status on hematoxylin and eosin (H&E) stained slides and the presence of IM and finally we examined the special stains. Results: 23% of cases were H. pylori-positive

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5

Jyoti, Ravi, Wadhwan Vijay, Sharma Preeti, and Yadav Lalita. "Qualitative analysis of keratin in oral squamous cell carcinoma using alcian blue–periodic acid schiff, Ayoub-Shklar, and modified papanicolaou as special stains." Journal of Oral and Maxillofacial Pathology 29, no. 1 (2025): 61–65. https://doi.org/10.4103/jomfp.jomfp_203_24.

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Abstract Background: The morphological features of keratinisation can impact histopathological diagnosis. Routine haematoxylin and eosin (H&E) stain has its limitations in moderately differentiated cases of oral squamous cell carcinoma (OSCC). Aims: The aim of this study was to evaluate and compare the characteristics of staining and identification of keratin by routine H&E stain, Alcian Blue–Periodic Acid Schiff (AB-PAS), Ayoub-Shklar, and Modified Papanicolaou (PAP) stain in OSCC cases. Material and Methods: Overall, 30 cases of OSCC were included in experimental groups, and 20 cases

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Madhusmita, Choudhury, Kumar Deka Monoj, Das Arindam, Das Soumistha, and Ahmed Choudhury Salman. "A Clinicopathological Study of Patients with Cutaneous Granulomatous Lesions with Reference to Special Stain." International Journal of Pharmaceutical and Clinical Research 16, no. 2 (2024): 743–57. https://doi.org/10.5281/zenodo.11074365.

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Granulation reaction pattern is defined as “Granulomatous inflammation is a form of chronic inflammation characterized by collections of activated macrophages, often with T lymphocytes, and sometimes associated with central necrosis”. Granulomatous disease comprises some of the widespread diseases in the world such as leprosy and tuberculosis. Granulomatous inflammation in the skin could be due to infectious or non-infectious causes. Common infectious causes of granulomatous skin lesions include leprosy, TB, leishmaniasis and fungal infections. Uses of special stains such as ZN sta

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Tandon, Megha, Sanjay Singh, and Ragini Tilak. "Comparison of different methods to determine the viability of dermatophytes in skin scrapings." IP Indian Journal of Clinical and Experimental Dermatology 9, no. 1 (2023): 49–54. http://dx.doi.org/10.18231/j.ijced.2023.008.

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One of the most prevalent infectious dermatoses seen in dermatology outpatient clinics is superficial fungal infections of the skin, hair, and nails. Filamentous fungi, dermatophytes, which invade and colonise the keratinised layers of the skin, are the main cause of skin fungal infection. Sometimes there is a discrepancy between the results of microscopy and culture for dermatophytes. If the microscopy of scrapings is positive due to presence of non-viable fungus, no growth will take place in culture. Culture is the method used to determine the presence of viable dermatophytes. However, the p

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8

Hao, Xingpei, Joon Yim, David Freedman, et al. "PAS stain based histological classification and severity grading of toenail onychomycosis." Medical Mycology 58, no. 4 (2019): 453–59. http://dx.doi.org/10.1093/mmy/myz075.

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Abstract Onychomycosis is a common world-wide health issue. Accurate detection is essential for treatment. Multiple studies have shown that PAS-stain based histological visualization of fungal elements is superior to either direct microscopy with 20% potassium hydroxide, or fungal culture. However, PAS stain based histological classification and severity grading of onychomycosis are lacking in the literature. Here we reported a fungal detection rate of 47.87% based on an analysis of 13,805 toenails processed for H&E and PAS stains over a three year period. Based on the analysis of fungal d

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9

Levett, Kaitlyn, Carina Wasko, and A. Hafeez Diwan. "The Answer Is Blowin' in the Wind: Styling Trauma of the Hair Shaft as a Piedra Mimic." American Journal of Dermatopathology 47, no. 8 (2025): 638–39. https://doi.org/10.1097/dad.0000000000002990.

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Abstract: Hair shaft disorders/abnormalities are usually easily diagnosed with the help of in-office trichoscopy and, if necessary, electron microscopy. Here we present a distinctive case of damaged, stripped hair cuticles clinically mimicking white piedra in a patient with a history of weekly hair blowout treatments. We demonstrate that staining sampled hair shafts with Periodic Acid-Schiff stain (PAS) and Gomori Methamine Silver stain (GMS) stains can be helpful to highlight cuticle fraying in instances where other explanations for these pseudonits have been ruled out. These “balled up” cuti

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Warsi, Sumbul, Fatma Lubna, Medha Mani Gupta, and Ruquiya Afrose. "Histoplasmosis: A case report." IP Archives of Cytology and Histopathology Research 9, no. 2 (2024): 121–25. http://dx.doi.org/10.18231/j.achr.2024.025.

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Histoplasmosis is common in certain regions of America, Asia and Africa. Cutaneous manifestation of Histoplasma capsulatum shows a wide spectrum of lesions. Histopathology plays a very important role in the detection and confirmation of diagnosis. Special fungus stains such as GMS (Gomori’s methenamine silver stain) and PAS (Periodic acid Schiff) stains can be used for its study.

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Tsang, William Y. W., and John K. C. Chan. "Microwave-Assisted PAS Stain for Frozen Sections." American Journal of Surgical Pathology 16, no. 8 (1992): 809. http://dx.doi.org/10.1097/00000478-199208000-00012.

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Hebert, A., and G. Love. "PAS Staining Variability in Fungal Infections of Skin." American Journal of Clinical Pathology 162, Supplement_1 (2024): S112. http://dx.doi.org/10.1093/ajcp/aqae129.249.

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Abstract Introduction/Objective Developed in 1946 by J. F. A. McManus, the Periodic Acid Schiff (PAS) stain is among the most frequently used stains in histopathology as a cost-effective ancillary study for the detection of fungal organisms. Despite its wide use in dermatopathology, little research exists comparing PAS to its commonly used counterpart, Gomori methenamine silver (GMS), or to hematoxylin and eosin (H&E) alone. The variation in PAS staining between different fungi causing skin infections has been poorly studied. Methods/Case Report Eighteen cases of fungal skin infections fro

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13

Rodríguez-Tovar, Luis E., Alejandra Villarreal-Marroquín, Alicia M. Nevárez-Garza, et al. "Histochemical study of Encephalitozoon cuniculi spores in the kidneys of naturally infected New Zealand rabbits." Journal of Veterinary Diagnostic Investigation 29, no. 3 (2017): 269–77. http://dx.doi.org/10.1177/1040638716668559.

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Encephalitozoon cuniculi is an important microsporidian pathogen that is considered an emergent, zoonotic, and opportunistic. It infects both domestic and laboratory rabbits, generating severe chronic interstitial and granulomatous nephritis with fibrosis and granulomatous encephalitis. Encephalitozoonosis is diagnosed in paraffin-embedded sections by examining the spores in the host tissues. The spores are difficult to observe when the samples are stained with hematoxylin and eosin (H&E), particularly when there is an inflammatory reaction and tissue damage. The spores are easily mistaken

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Simoes, Jose Pedro Cannas, and Polly Schoning. "Canine Mast Cell Tumors: A Comparison of Staining Techniques." Journal of Veterinary Diagnostic Investigation 6, no. 4 (1994): 458–65. http://dx.doi.org/10.1177/104063879400600410.

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Twelve histochemical methods; affinity staining with avidin peroxidase, wheat germ agglutinin, and concavalin-A agglutinin; and an immunohistochemical stain with Kpl (CD68) antibody were compared for their relative effectiveness in staining canine mast cell tumors. Stains were compared in 28 mast cell tumors and 19 histiocytomas. The effectiveness of the histochemical methods and the lectins decreased as the mast cells became less differentiated. None of the staining methods were positive on histiocytomas. Periodic acid-Schiff (PAS) gave positive results in a few cases of mast cell tumors wher

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15

Khurshid Sipra, Muhammad Wajid, Zahid Mehmood Akhtar, and Tayyaba Batool. "Sensitivity of PAS histopathology stain for the diagnosis of onychomycosis at multicenter teaching hospitals, Pakistan." Professional Medical Journal 26, no. 08 (2019): 1213–17. http://dx.doi.org/10.29309/tpmj/2019.26.08.257.

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The aim of this study was to determine the sensitivity of Periodic acid –Schiff (PAS) staining an early and quick effective diagnostic test of nail clipping with routine tests in the diagnosis of Onychomycosis. Background: The routine gold standard for diagnosis of fungal nail infections has been direct microscopy (KOH mount) and mycological culture which often yield delayed or weak/false negative results. However recent studies have suggested that nail plate biopsy using PAS stain is rapid method of detection that grasped the diagnosis and manage the disease. Study Design: Cross sectional des

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Dworak, Otto, and Christian Wittekind. "A 30-S PAS Stain for Frozen Sections." American Journal of Surgical Pathology 16, no. 1 (1992): 87–88. http://dx.doi.org/10.1097/00000478-199201000-00014.

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De Rosa, Gaetano, Giovanna Giordano, Amedeo Boscaino, Luigi Terracciano, Vittoria Donofrio, and Gianfranco De Dominicis. "Intracystic Papillary Carcinoma of the Male Breast. A Case Report: (Histochemical, immunohistochemical and ultrastructural study)." Tumori Journal 78, no. 1 (1992): 37–42. http://dx.doi.org/10.1177/030089169207800109.

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We report a case of intracystic papillary carcinoma of the male breast in a 70-year-old male Caucasian. Grossly, the tumor was a cystic lesion measuring 6 cm in diameter. It contained hemorrhagic fluid and a mural nodule with filiform projections. PAS stain with and without digestion revealed small clumps of diastase-resistent material in the cytoplasm of the neoplastic cells. Grimelius stain was positive. Immunoperoxidase stains were negative for neuron-specific enolase, S100 protein, cromogranin and synaptophysin and were positive for carcinoembryonic antigen and epithelial membrane antigen.

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18

Chairani, Chairani, Fitra Wahyuni, Tofrizal Tofrizal, and Salsabila Salsabila. "STRUKTUR HISTOLOGI DAN JUMLAH SEL GOBLET PADA SEDIAAN HISTOPATOLOGIS RADANG USUS BESAR DENGAN PEWARNAAN HEMATOXYLIN-EOSIN (HE) DAN PERIODIC ACID-SCHIFF (PAS)." Ensiklopedia of Journal 4, no. 3 (2022): 182–86. http://dx.doi.org/10.33559/eoj.v4i3.137.

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Abstract: Goblet cells and the mucus they produce serve as an important barrier to prevent pathogens invading the mucosal tissue that can cause inflammation. In the inflamed tissue, will have changed in the structure and number of goblet cells. Hematoxylin-Eosin (HE) staining is a standard stain used to examine the histological structure of tissues while Periodic Acid-Schiff (PAS) is a histological stain other than HE that can stain carbohydrates and carbohydrate-rich macromolecules. The purpose of this study was to observe differences in the structure and number of goblet cells in the inflamm

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Altenburg, T. M., H. Degens, W. van Mechelen, A. J. Sargeant, and A. de Haan. "Recruitment of single muscle fibers during submaximal cycling exercise." Journal of Applied Physiology 103, no. 5 (2007): 1752–56. http://dx.doi.org/10.1152/japplphysiol.00496.2007.

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In literature, an inconsistency exists in the submaximal exercise intensity at which type II fibers are activated. In the present study, the recruitment of type I and II fibers was investigated from the very beginning and throughout a 45-min cycle exercise at 75% of the maximal oxygen uptake, which corresponded to 38% of the maximal dynamic muscle force. Biopsies of the vastus lateralis muscle were taken from six subjects at rest and during the exercise, two at each time point. From the first biopsy single fibers were isolated and characterized as type I and II, and phosphocreatine-to-creatine

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Hajar, Tamar, Ramon Fernández-Martínez, Gabriela Moreno-Coutiño, Elsa Vásquez del Mercado, and Roberto Arenas. "Modified PAS stain: A new diagnostic method for onychomycosis." Revista Iberoamericana de Micología 33, no. 1 (2016): 34–37. http://dx.doi.org/10.1016/j.riam.2014.10.002.

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Gowda, R. Yashica, and Thejasvi Krishnamurthy. "Histomorphological Study of Nail Changes in Various Dermatoses — An Understudied Entity in Dermatopathology." Journal of Medical Sciences and Health 8, no. 3 (2022): 252–57. http://dx.doi.org/10.46347/jmsh.v8i3.22.98.

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Background: The nail is our most versatile tool and serves a very important function of protecting the distal phalanx. The nail unit shows specific changes that are markers for a wide range of local and systemic conditions. Objectives: The objectives of the study are to identify the spectrum of histomorphological features in various nail lesions and to highlight the role of histopathology in diagnosis of nail diseases. Methods: This is a descriptive study done over a period of 18 months. The nail biopsy specimens were received in 10 % formalin, softened with 10% nitric acid, processed and stai

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Audon, P., H. Sigurdarson, Y. Guzman, et al. "Immunohistochemical diagnosis of onychomycosis by monoclonal antibodies detection of dermatophyte T. rubrum." American Journal of Clinical Pathology 156, Supplement_1 (2021): S45—S46. http://dx.doi.org/10.1093/ajcp/aqab191.092.

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Abstract Introduction/Objective Onychomycosis is one of the most common nail infection disorders, caused predominantly by T. rubrum. Currently, PAS stain is the gold standard for histological onychomycosis detection. However, it does not differentiate between the types of fungi. In addition, PAS stains will only provide a positive or negative result. This leaves physicians to prescribe medications that may be ineffective in treating the root cause of the infection. By discerning the causative specie, physicians can provide a more targeted and effective anti-fungal therapy. A novel monoclonal a

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M. Patel, Mandakini, Bhavna N. Gamit, Sharmistha M. Patel, Mubin I. Patel, and Shivangi S. Gandhi. "Acinic cell carcinoma, papillary-cystic variant: a rare case diagnosed in fine needle aspiration cytology." International Journal of Research in Medical Sciences 6, no. 3 (2018): 1046. http://dx.doi.org/10.18203/2320-6012.ijrms20180638.

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Acinic Cell Carcinoma is a rarest malignant epithelial neoplasm arising from pleuripotent stem cells. ACC constitute 6% of all salivary gland tumor. Most common site is parotid. It is slowly growing tumor with female preponderance most commonly occur during fifth and sixth decades of life. Diagnosis by imaging study are complex, but USG is helpful in evaluating tumor. We are presenting a 9year female, complaint of a painless, freely movable, atraumatic swelling on left cheek measuring 2x1cm2, slowly enlarging since, 8 months. FNAC was performed from the swelling and routine stain (H and E, MGG

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Salahudeen, Mohamed, Anita Ramdas, Arthi Elumalai, Kandasamy Ravichandran, and Sheela Kuruvila. "Efficacy of periodic acid–Schiff stain in the diagnosis of onychomycosis – A cross-sectional study." Journal of Current Research in Scientific Medicine 8, no. 1 (2022): 32–36. http://dx.doi.org/10.4103/jcrsm.jcrsm_3_22.

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Background: Onychomycosis is the fungal infection of the nail which needs to be differentiated from other causes of dystrophic nails due to its long-term treatment. Direct microscopic examination with potassium hydroxide (KOH) mount along with fungal culture shows inconsistent sensitivity. Thus, there is a need of more accurate and efficient method to detect onychomycosis. Aims and Objectives: This study aims to compare periodic acid–Schiff (PAS) stain with KOH and nail fungal culture in the diagnosis of onychomycosis. Materials and Methods: This was a cross-sectional study conducted in the de

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Maysoon Faraj Elrashdy, Rima Ahmed Benomran, Nabeia Ali Gheryani, and Abeer Hussien Amer. "A histopathological study on the effects of cyclophosphamide on the hepatic tissue of female golden hamsters." International Journal of Frontiers in Life Science Research 3, no. 1 (2022): 022–29. http://dx.doi.org/10.53294/ijflsr.2022.3.1.0047.

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The aim of this study was to study the acute toxic effect of cyclophosphamide on the histological structure of the liver tissue in Syrian golden hamsters. Twenty-seven adult female hamsters were injected intraperitoneally with 0, 100 and 200 mg/kg body weight cyclophosphamide. The dosing was administrated on alternating days for one week as an acute dosing schedule and humane sacrifice was done on day 7. Hepatic tissue was collected and processed with paraffin technique for histological examination then stained with hematoxylin and eosin stains (H&E) and Periodic acid–Schiff stain (PAS). T

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Al-shiaty, Rami A., and Bacem A. E. Ottoman. "Recurrent pyogenic granuloma: an update." International Journal of Scientific Reports 1, no. 1 (2015): 22. http://dx.doi.org/10.18203/issn.2454-2156.intjscirep20150196.

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<p class="abstract"><strong><span lang="EN-US">Background: </span></strong>Given the fierce controversy about the nature of pyogenic granulomas, starting with its unfitting name and ending up with its ideal treatment modality, this paper tries to numerically identify some predisposing factors of recurrence.</p><p class="abstract"><strong><span lang="EN-US">Methods: </span></strong>The literature was initially reviewed and a total of twenty recurrent cases of pyogenic granuloma were contrasted, on one hand, to their initial appea

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Sindhoor, Sharada Rai, and Deepa Adiga S A. "Periodic acid-schiff staining: A counterpart of the immunoreactivity seen by direct immunofluorescence in bullous pemphigoid." Indian Journal of Pathology and Oncology 11, no. 3 (2024): 238–42. http://dx.doi.org/10.18231/j.ijpo.2024.054.

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Periodic-Acid-Schiff (PAS) stain has been put forward to differentiate between bullous pemphigoid (BP) and epidermolysis bullosa acquisita (EBA). PAS stain shows correspondence with DIF staining patterns of EBA and BP with respect to basement membrane staining. Histological hall-mark of this entity is subepidermal blister containing eosinophils. But there can be other findings observed in this entity which can cause pitfalls in the diagnosis. Our study demonstrates these rare findings to avoid the misdiagnosis.To study the utility of Periodic-acid-Schiff stain in differentiating BP and EBA on

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Sood, Ridhi, Ruchita Tyagi, Pavneet Kaur Selhi, Gursheen Kaur, Harpreet Kaur, and Akashdeep Singh. "Role of FNA and Special Stains in Rapid Cytopathological Diagnosis of Pulmonary Nocardiosis." Acta Cytologica 62, no. 3 (2018): 178–82. http://dx.doi.org/10.1159/000488134.

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Background: Nocardia, a gram-positive aerobic bacillus of the Actinomycetales family, is a significant opportunistic pathogen in immunocompromised individuals. Clinical and radiological features of pulmonary nocardiosis are nonspecific and can be misdiagnosed as tuberculosis, pneumocystis, staphylococcal or fungal infections, or as malignancy. Aspiration cytology with special stains is a quick and effective approach for accurate diagnosis. Materials and Methods: We present 7 cases of pulmonary nocardiosis, admitted to the pathology department in a tertiary-care hospital in Punjab. Clinical fin

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Ponmar, Madhurima, Dharshini Sathishkumar, Susanne Alexander Pulimood, and Meera Thomas. "Patterns of Periodic Acid–Schiff Staining in Subepidermal Bullous Disorders with Emphasis on Bullous Pemphigoid." Current Medical Issues 22, no. 1 (2024): 1–6. http://dx.doi.org/10.4103/cmi.cmi_69_23.

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Abstract Background: Direct immunofluorescence (DIF) including salt split skin technique is quintessential in the diagnosis of subepidermal bullous disorders. These techniques are expensive and require technical expertise, limiting their diagnostic utility. The property of periodic-acid–Schiff to stain the basement membrane and the patterns produced by it was observed in a cohort of subepidermal bullous disorders, to see if it offers an alternative cost-effective testing method. Materials and Methods: This was a retrospective, observational study conducted in the department of general patholog

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Saxena, Rushali, Smita Singh, and Kiran Agarwal. "Gastric amphicrine carcinoma: A histopathological diagnostic conundrum." Indian Journal of Pathology and Oncology 10, no. 2 (2023): 191–94. http://dx.doi.org/10.18231/j.ijpo.2023.040.

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Amphicrine carcinoma is rarely reported in literature. It is characterised by dual positivity of neuroendocrine markers and mucin stain in the same cells depicting divergent differentiation. Recently published WorldHealth Organisation (WHO) classification of neuroendocrine neoplasm of 2022 differentiates it from adenocarcinoma and mixed neuroendocrine-nonneuroendocrine neoplasms (MiNEN) it shows varied histomorphology and on rare occasions neuroendocrine features may not be evident in poorly differentiated carcinoma. Hence, diagnosis based solely on histomorphology can be misleading. This is a

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Liaquat, S., A. Bryson, and M. Mochel. "Examination Of Nail Clippings For Onychomycosis: Histologic Observations With Microbiologic Correlations." American Journal of Clinical Pathology 154, Supplement_1 (2020): S42. http://dx.doi.org/10.1093/ajcp/aqaa161.088.

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Abstract Introduction/Objective Appropriate management of onychomycosis, the most common disease of the nail unit, depends on accurate and timely diagnosis. Histologic evaluation of nail clippings, paired with histochemical techniques such as Periodic acid-Schiff (PAS), is a rapid, sensitive method for diagnosis of onychomycosis. While studies have compared testing modalities for onychomycosis, few have systematically evaluated histologic changes of the nail plate and correlated findings with concomitant microbiologic culture. Methods A consecutive series of nail clipping specimens between Jul

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Oliveira, L. A., E. A. Viscone, A. A. Medeiros-Ronchi, and M. B. Bandarra. "Histopathological features of the brain extracellular matrix from dogs with canine distemper." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 75, no. 5 (2023): 831–42. http://dx.doi.org/10.1590/1678-4162-12651.

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ABSTRACT Canine distemper causes demyelinating leucoencephalitis, like human multiple sclerosis. The encephalic microenvironment, including the extracellular matrix, is an important key factor of this lesion, already described in multiple sclerosis but not proved in canine distemper. Thereby, the aim of this work is to characterize the extracellular matrix in the encephalon of dogs with canine distemper. Samples of cortex and cerebellum of 14 naturally infected dogs with canine distemper virus were collected after being sent for necropsy in the Animal Pathology Laboratory of the Veterinary Hos

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Majeed, Muhammad Mudassar, and Mulazim Hussain Bukhari. "Evaluation for Granulomatous Inflammation on Fine Needle Aspiration Cytology Using Special Stains." Pathology Research International 2011 (June 30, 2011): 1–8. http://dx.doi.org/10.4061/2011/851524.

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Background. Tuberculosis is the commonest infectious disease in the developing world. Many diagnostic tests are devised for its detection including direct smear examination. This study was designed to determine the frequency of cases positive for AFB and positive for fungus in patients diagnosed to have granulomatous inflammation on Fine Needle Aspiration Cytology using special stains. Materials and Methods. A descriptive cross-sectional survey was done on 100 cases of granulomatous inflammation consistent with tuberculosis diagnosed on fine needle aspiration cytology at the Department of Path

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Yassin, Asseel. "Histomorphometric Study of the Thyroid Gland of the Adult Male Squirrel (Sciurus Anomalus)." Kufa Journal For Veterinary Medical Sciences 15, no. 1 (2024): 1–9. http://dx.doi.org/10.36326/kjvs/2024/v15i114552.

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The aim of the current study is to determine the histological and histochemical characteristics of the thyroid glands in local male squirrels. So, thyroid gland tissue was freshly collected from an adult healthy local male squirrel for histological preparation and examination. All histological sections were prepared and stained with routine stains H & E, in addition to specific stains including Masson Trichrome and PAS stains. The histological results showed that thyroid glands consisted of glandular parenchyma enclosed by a capsule which mainly consisted of collagen fibers and sent septa

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Farhan, Noor, and Ahmed Hussein. "Histological comparison of pancreas and duodenum in Barn Owl, Moorhen and Budgie birds." Tikrit Journal for Agricultural Sciences 25, no. 1 (2025): 88–100. https://doi.org/10.25130/tjas.25.1.7.

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The study aimed to describe the histological features of Pancreas and Duodenum in Common Moorhen (Galinula choropus), Barn Owl (Tyto alba) and common Budgie (Melopsittacus undulatus). Compare histological parameters of Pancreas and Duodenum among these birds. In the current study, Histochemistry of Pancreas showed that the use of Masson trichrome staining in Owl’s pancreas shows presence of collagen fibers in the septa between lobes and also within the capsule surrounded the organ. while this stain shows positive reaction only in capsule in Moorhen and Budgie bird pancreas. Owl bird and budger

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Kobayashi, Naomi, Thomas W. Bauer, Daisuke Togawa, et al. "A Molecular Gram Stain Using Broad Range PCR and Pyrosequencing Technology." Diagnostic Molecular Pathology 14, no. 2 (2005): 83–89. http://dx.doi.org/10.1097/01.pas.0000162753.38284.1a.

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Lin, Juan, Zhu-Feng Chen, Guo-Dong Guo, and Xin Chen. "Impact of Alcian blue and periodic acid Schiff expression on the prognosis of gastric signet ring cell carcinoma." World Journal of Gastrointestinal Oncology 16, no. 3 (2024): 687–98. http://dx.doi.org/10.4251/wjgo.v16.i3.687.

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BACKGROUND The Alcian blue (AB) and periodic acid Schiff (PAS) stains are representative mucus markers in gastric signet ring cell carcinoma (SRCC). They are low-cost special staining methods used to detect acidic mucus and neutral mucus, respectively. However, the clinical importance of the special combined AB and PAS stain is unclear. AIM To investigate AB expression, PAS expression and the AB-to-PAS (A/P) ratio in gastric SRCC patients and to assess patient prognosis. METHODS Paraffin-embedded sections from 83 patients with gastric SRCC were stained with AB and PAS, and signet ring cell pos

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Shivji, Sameer, Ipshita Kak, Stephanie L. Reid, et al. "Feasibility and Performance of Elastin Trichrome as a Primary Stain in Colorectal Cancer Resection Specimens." American Journal of Surgical Pathology 45, no. 10 (2021): 1419–27. http://dx.doi.org/10.1097/pas.0000000000001707.

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Liang, Wen-Yih, Wei-Chin Chang, Chih-Yi Hsu, et al. "Retrospective Evaluation of Elastic Stain in the Assessment of Serosal Invasion of pT3N0 Colorectal Cancers." American Journal of Surgical Pathology 37, no. 10 (2013): 1565–70. http://dx.doi.org/10.1097/pas.0b013e31828ea2de.

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Crist, Henry, Max Hennessy, Jacob Hodos, et al. "Acute Invasive Fungal Rhinosinusitis: Frozen Section Histomorphology and Diagnosis with PAS Stain." Head and Neck Pathology 13, no. 3 (2018): 318–26. http://dx.doi.org/10.1007/s12105-018-0965-8.

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Alfahaad, Hamad, Hamza Alshehri, Ebtisam Aljerayan, Albaraa Alwabel, and Abdullah Mushabeb Alfarwan. "Successful Treatment of Lipoid Proteinosis in a Young Adult with Isotretinoin." Journal of Pharmaceutical Research International 35, no. 10 (2023): 29–33. http://dx.doi.org/10.9734/jpri/2023/v35i107351.

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Lipoid proteinosis (LP) is an extremely rare autosomal recessive genodermatosis, characterized by persistent hoarseness and classical skin lesions. Amorphous hyaline depositions may affect any organ, but mainly the face and extremities. These depositions cause limitations in tongue movement, and hence, difficulty speaking. Biopsies of skin lesions usually show massive deposits of homogeneous, acidic, hyaline-like material at the epidermal junctions and superficial dermis that stains positive for periodic acid-Schiff stain (PAS-D). At present, there is no specific treatment for this extremely r

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Arvind, Chouhan, and Ahmad Fuzail. "Evaluation for Granulomatous Inflammation on Fine Needle Aspiration Cytology using Special Stains." International Journal of Pharmaceutical and Clinical Research 15, no. 4 (2023): 1371–83. https://doi.org/10.5281/zenodo.12680527.

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<strong>Background:</strong> Tuberculosis is the commonest infectious disease in the developing countries like India. Many diagnostic tests are devised for its detection including direct smear examination. This study was designed to determine the frequency of cases positive for AFB and positive for fungus in patients diagnosed to have granulomatous inflammation on Fine Needle Aspiration Cytology using special stains. <strong>Materials and Methods: </strong>A descriptive cross-sectional survey was done on 100 cases of granulomatous inflammation consistent with tuberculosis diagno

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Erkan, Melike, and Yasemin Ayun. "Morphological and histochemical examination of male and female gonads in Homarus gammarus (L. 1758)." Open Life Sciences 9, no. 1 (2014): 37–48. http://dx.doi.org/10.2478/s11535-013-0148-7.

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AbstractThe reproductive organs of both male and female European lobsters (Homarus gammarus) are H-shaped gonads that lie dorsal to the gut on the large hepatopancreas. The ovary consists of a pair of tubular, parallel lobules with a connecting bridge. The germarium of the ovary containing oogonia is concentrated in the center of the ovarian lobe. As oogonesis proceeds, the oocytes move to the peripheral regions of the ovary. The follicle cells begin to surround the oocytes in the previtellogenic stage, and the mature oocytes are completely surrounded by the follicle cells. Carbohydrates exist

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Karim, Abdulkarim, Ali Hassan, Khalid Kadhim, and Khalid Kadhim. "The immunity function of rodlet cells in the intestine of Binni fish (Mesopotamichthys sharpeyi)." Journal of Advanced Veterinary and Animal Research 9, no. 2 (2022): 282. http://dx.doi.org/10.5455/javar.2022.i594.

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Objective: Rodlet cells produce secretions of glycoproteins in nature. This study investigated the microscopic morphology, histochemical and immunohistochemical reactions, and distribution of the rodlet cells in the gut of Binni fish (Mesopotamichthys sharpeyi). Materials and Methods: Thirty samples were obtained from the cranial, middle, and caudal por¬tions of Binni intestine immediately after being euthanized, fixed in Bouin’s solution for 18 h at 24°C, and had undergone routine histological processing, different conventional histochemical stains, and immunostaining with TNF-α and S100 prot

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Lee, You-Jin, Noriyuki Nagai, Chong-Huat Siar, et al. "Angioarchitecture of Primary Oral Malignant Melanomas." Journal of Histochemistry & Cytochemistry 50, no. 11 (2002): 1555–62. http://dx.doi.org/10.1177/002215540205001116.

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Angiogenesis is an essential process in the progression of malignant tumors. However, little is known of the angioarchitecture in primary oral malignant melanoma. We sought to determine this by the use of periodic acid-Schiff (PAS) stain, endothelial markers (CD34, CD105) and laminin, and by transmission electron microscopy in two cases. The results demonstrated that endothelium-lined vessels dominated the tumor microvasculature and these stained positively for PAS, laminin, and endothelial markers. Mosaic and tumorlined vessels were infrequently encountered. Most PAS-positive patterned networ

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El Jalkh, Jason, Pia Maria Obeid, Dorra Guermazi, Aya Soubra, and Elie Saliba. "Skin-Colored Papules on the Neck of a Postmenopausal Woman: A Diagnostic Challenge." Dermatopathology 12, no. 2 (2025): 15. https://doi.org/10.3390/dermatopathology12020015.

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A 64-year-old patient presented for management of symptomatic skin-colored papules symmetrically distributed over the lateral neck over the past two years, which failed to improve on multiple topical corticosteroids, antifungal creams, and topical calcineurin inhibitor. Histopathologic examination showed a regular epidermis with increased melanophages in the papillary dermis, without vacuolar degeneration of the basement membrane. Verhoeff Van Gieson stain highlighted a band-like zone of attenuated elastic fibers in the papillary dermis, while Von Kossa stain was negative for calcified fibers.

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Lafta, Israa, Wasan Abdulhameed, and Nahla AL-Bakri. "Histochemical Study of Human Placental Tissues in Gestational Diabetic Mellitus." Iraqi Journal of Embryos and Infertility Researches 10, no. 2 (2021): 29–38. http://dx.doi.org/10.28969/ijeir.v10.i2.r3.

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Gestational diabetes mellitus (GDM) is a serious pregnancy complication in which a woman who has never had diabetes develops chronic hyperglycemia during her pregnancy. Normal placental function is essential for optimal fetal growth. The transport of glucose from the mother to the fetus is critical for fetal nutrient demands and can be stored as glycogen in the placenta. However, the function of this glycogen deposition is unknown: It may well be a source of fuel for a placenta itself or the storage reservoir for the later use by the fetus in times of need. While the significance of the placen

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Rafal Yousuf and Azhar saleem Khalaf. "Histomorphological and histochemical Study of the jejunum in moorhen (Gallinula chloropus) in Iraq." University of Thi-Qar Journal of agricultural research 12, no. 2 (2023): 120–30. http://dx.doi.org/10.54174/utjagr.v12i2.272.

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The goal of the current investigation was to gather information on the histology and histochemical composition of the jejunum in moorhens (Gallinula chloropus). In order to conduct the current investigation, six moorhens were gathered. They were purchased from regional vendors in particular markets in Baghdad, Iraq. To collect the intestine, birds were put down after being dissected, and the specimens were then fixed in 10% neutral buffered formalin. The blocks were sectioned at 6 m thickness and stained with Mayer's hematoxylin and eosin, Masson trichrome stain, periodic acid Schiff reagent (

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Ahmed Lafta, Israa, Wasan Adnan Abdulhameed, and Nahla A. AL-Bakri. "Histochemical Study of Human Placental Tissues in Gestational Diabetic Mellitus." IraQi Journal of Embryos and Infertility Researches 11, no. 2 (2021): 29–38. http://dx.doi.org/10.28969/ijeir.v11.i2.r3.

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Gestational diabetes mellitus (GDM) is a serious pregnancy complication in which a woman who has never had diabetes develops chronic hyperglycemia during her pregnancy. Normal placental function is essential for optimal fetal growth. The transport of glucose from the mother to the fetus is critical for fetal nutrient demands and can be stored as glycogen in the placenta. However, the function of this glycogen deposition is unknown: It may well be a source of fuel for a placenta itself or the storage reservoir for the later use by the fetus in times of need. While the significance of the placen

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Obead, J. "Histochemical study of Hydatid cyst isolated from sheep within the abattoirs Kerbala province, Iraq." Scientific Progress & Innovations 28, no. 1 (2025): 182–86. https://doi.org/10.31210/spi2025.28.01.28.

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Hydatid Cysts of Echinococcosis (CE) is a highly endemic parasitic zoonotic disease in Iraq that has a significant effect on the animals production (sheep, cattle, and goats) and human general health. It can affect any organ in the body, particularly the liver and lungs of the intermediate host. Carnivorous animals, notably dogs, operate as the definitive host for Echinococcus granulosus, whereas herbivorous animals, mostly sheep, are considered the intermediate host. Twenty liver samples afflicted with hydatid disease were acquired for this investigation, which employed a range of specialized

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