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Artykuły w czasopismach na temat „PBR322”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Bharathi, A., та H. Polasa. "Elimination of ColE1(pBR322 and pBR329) plasmids inEscherichia coliby α-santonin". FEMS Microbiology Letters 68, № 1-2 (1990): 213–15. http://dx.doi.org/10.1111/j.1574-6968.1990.tb04151.x.

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2

FRENKEL, L., and H. BREMER. "Increased Amplification of Plasmids pBR322 and pBR327 by Low Concentrations of Chloramphenicol." DNA 5, no. 6 (1986): 539–44. http://dx.doi.org/10.1089/dna.1.1986.5.539.

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3

Takahashi, H., H. Kojima, and H. Saito. "A new site-specific endonuclease, ScaI, from Streptomyces caespitosus." Biochemical Journal 231, no. 1 (1985): 229–32. http://dx.doi.org/10.1042/bj2310229.

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A new site-specific endonuclease has been isolated from Streptomyces caespitosus and named ScaI. Based on analysis of sequences around the restriction sites in pBR322 and pBR325, the recognition sequence of ScaI endonuclease was deduced to be a new hexanucleotide 5′-AGTACT-3′. The cleavage site was determined by comparing the ScaI-cleaved product of a primer-extended M13mp18-SCA DNA, which contains an AGTACT sequence, with dideoxy chain terminator ladders of the same DNA. ScaI was found to cleave the recognition sequence between the internal T and A, leaving flush ends to the cleaved fragments

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4

Guzman, Elena C., Francisco J. Carrillo, and Alfonso Jimenez-Sanchez. "Differential inhibition of the initiation of DNA replication in stringent and relaxed strains ofEscherichia coli." Genetical Research 51, no. 3 (1988): 173–77. http://dx.doi.org/10.1017/s0016672300024265.

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SummaryStarvation for isoleucine inhibits chromosome, minichromosome and pBR322 DNA replication in a stringent strain ofE. coli, but does not do so in a relaxed mutant. Starvation for other amino acids inhibits either chromosome and minichromosome replication in both strains. From these results we conclude thatoriCand pBR322 replication are stringently regulated and that isoleucine seems not to be essential for the protein synthesis required at the initiation oforiCreplication. Deprivation of isoleucine in a Rel−strain gives rise to amplification of minichromosome and pBR322 with a better yiel

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5

Kuhn, S., C. E. Vorgias, and P. Traub. "Interaction in vitro of non-epithelial intermediate filament proteins with supercoiled plasmid DNA." Journal of Cell Science 87, no. 4 (1987): 543–54. http://dx.doi.org/10.1242/jcs.87.4.543.

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Sucrose gradient analysis of reaction products obtained from non-epithelial intermediate filament (IF) subunit proteins and a mixture of supercoiled, relaxed and linearized plasmid pBR322 DNA at low ionic strength revealed that limited amounts of these polypeptides interacted exclusively with the supercoiled form of the plasmid DNA. These results were corroborated by electron-microscopic analysis of the reaction products, which showed that only circles of supercoiled pBR322 DNA were completely and smoothly covered with vimentin. IFs reconstituted from pure vimentin reacted with supercoiled pBR

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6

Lakshmi, V. V., and H. Polasa. "Curing of pBR322 and pBR329 plasmids inEscherichia colibycis-dichlorodiamine platinum(II) chloride (Cis-DDP)." FEMS Microbiology Letters 78, no. 2-3 (1991): 281–84. http://dx.doi.org/10.1111/j.1574-6968.1991.tb04456.x.

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7

Chiang, C. S., and H. Bremer. "Stability of pBR322-derived plasmids." Plasmid 20, no. 3 (1988): 207–20. http://dx.doi.org/10.1016/0147-619x(88)90027-3.

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8

Trevors, J. T., J. D. Van Elsas, M. E. Starodub, and L. S. Van Overbeek. "Survival of and plasmid stability in Pseudomonas and Klebsiella spp. introduced into agricultural drainage water." Canadian Journal of Microbiology 35, no. 7 (1989): 675–80. http://dx.doi.org/10.1139/m89-110.

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Cell survival and plasmid stability in Pseudomonas fluorescens R2f and Pseudomonas putida CYM 318 containing respectively, plasmid RP4 and pRK2501, and Klebsiella aerogenes NCTC 418 harboring plasmid pBR322 were studied in sterile and nonsterile agricultural drainage water under both aerobic and anaerobic conditions and in the absence and presence of added nutrients. Both Pseudomonas strains survived well in sterile drainage water incubated aerobically, with or without added nutrients. However, Klebsiella aerogenes NCTC 418 (pBR322) only survived in the presence of added nutrients. Pseudomonas

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9

Suryowati, Trini, John Jackson Yang, Lusia Sri Sunarti, and Maria Bintang. "The Effect of Torbangun (Coleus amboinicus Lour) Leaf Extract on Antibiotic Resistant Bacteria Escherichia coli pBR322 and Toxicity Tests on Artemia salina Leach." Indonesian Journal of Applied Research (IJAR) 5, no. 1 (2024): 1–8. http://dx.doi.org/10.30997/ijar.v5i1.388.

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Inappropriate antibiotic use can lead to antibiotic resistance, causing many problems in the treatment of Escherichia coli infection. One way to avoid resistance is to use the traditional medicine Coleus amboinicus Lour, also known as Torbangun in Indonesia. The purpose of this study was to determine the inhibitory ability of 70% ethanol extract of Torbngun leaf against Escherichia coli pBR322 has plasmid pBR322, which is resistant to antibiotics Ampicillin and Tetracycline based on activity and minimum inhibitory concentration. Extraction was carried out using the maceration method. The prese

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10

Yakushevich, Ludmila V., and Larisa A. Krasnobaeva. "Double energy profile of pBR322 plasmid." AIMS Biophysics 8, no. 2 (2021): 221–32. http://dx.doi.org/10.3934/biophy.2021016.

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11

Cronan, John E. "pBR322 vectors having tetracycline-dependent replication." Plasmid 84-85 (March 2016): 20–26. http://dx.doi.org/10.1016/j.plasmid.2016.02.004.

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12

Weston-Hafer, Kathleen, and Douglas E. Berg. "Specificity of deletion events in pBR322." Plasmid 21, no. 3 (1989): 251–53. http://dx.doi.org/10.1016/0147-619x(89)90050-4.

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13

Parveen, Sabiha, Mohammad Usman, Sartaj Tabassum, and Farukh Arjmand. "Synthesis and characterization of Co(ii) and Fe(ii) peptide conjugates as hydrolytic cleaving agents and their preferential enantiomeric disposition for CT-DNA: structural investigation of l-enantiomers by DFT and molecular docking studies." RSC Advances 5, no. 88 (2015): 72121–31. http://dx.doi.org/10.1039/c5ra15742k.

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14

Kumbhar, Amar S., Shirish G. Damle, Suryasarathi T. Dasgupta, Sandhya Y. Rane, and Avinash S. Kumbhar. "Nuclease Activity of Oxo-bridged Diiron Complexes." Journal of Chemical Research 23, no. 2 (1999): 98–99. http://dx.doi.org/10.1177/174751989902300216.

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15

Parveen, Sabiha, Sartaj Tabassum, and Farukh Arjmand. "Synthesis of chiral R/S-pseudopeptide-based Cu(ii) & Zn(ii) complexes for use in targeted delivery for antitumor therapy: enantiomeric discrimination with CT-DNA and pBR322 DNA hydrolytic cleavage mechanism." RSC Advances 7, no. 11 (2017): 6587–97. http://dx.doi.org/10.1039/c6ra24770a.

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16

Tsuge, Kenji, and Mitsuhiro Itaya. "Recombinational Transfer of 100-Kilobase Genomic DNA to Plasmid in Bacillus subtilis 168." Journal of Bacteriology 183, no. 18 (2001): 5453–58. http://dx.doi.org/10.1128/jb.183.18.5453-5458.2001.

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ABSTRACT Transformation of Bacillus subtilis by a plasmid requires a circular multimeric form. In contrast, linearized plasmids can be circularized only when homologous sequences are present in the host genome. A recombinational transfer system was constructed with this intrinsic B. subtilis recombinational repair pathway. The vector, pGETS103, a derivative of the θ-type replicating plasmid pTB19 of thermophilic Bacillus, had the full length of Escherichia coli plasmid pBR322. A multimeric form of pGETS103 yielded tetracycline-resistant transformants ofB. subtilis. In contrast, linearized pGET

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17

Mann, Brandon A., and James M. Slauch. "Transduction of Low-Copy Number Plasmids by Bacteriophage P22." Genetics 146, no. 2 (1997): 447–56. http://dx.doi.org/10.1093/genetics/146.2.447.

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The generalized transducing bacteriophage of Salmonella typhimurium, P22, can transduce plasmids in addition to chromosomal markers. Previous studies have concentrated on transduction of pBR322 by P22 and P22HT, the high transducing mutant of P22. This study investigates the mechanism of P22HT transduction of low-copy number plasmids, namely pSC101 derivatives. We show that P22HT transduces pSC101 derivatives that share homology with the chromosome by two distinct mechanisms. In the first mechanism, the plasmid integrates into the chromosome of the donor by homologous recombination. This chrom

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18

Santika, I. Wayan Martadi, and Putu Eka Arimbawa. "Amplifikasi Gen Bla Dan Ori Untuk Konstruksi Vektor Ekspresi Vaksin Rekombinan Dengan Metode PCR." Syntax Literate ; Jurnal Ilmiah Indonesia 7, no. 1 (2022): 292. http://dx.doi.org/10.36418/syntax-literate.v7i1.6054.

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Vaksin rekombinan subunit protein memiliki tingkat keamanan yang lebih baik dibandingkan vaksin yang dilemahkan atau dimatikan sehingga terdapat peningkatan kebutuhan vaksin tersebut. Peningkatan kebutuhan ini harus diantisipasi dengan peningkatan kapasitas produksi. Vektor ekspresi yang memiliki efisiensi transkripsi yang tinggi dan membawa ori dengan jumlah salinan rendah-sedang dapat meningkatkan kapasitas produksi vaksin rekombinan subunit protein. Penelitian ini bertujuan untuk mengamplifikasi gen bla dan ori dari plasmid pBR322 dengan jumlah salinan rendah-sedang dengan metode PCR. Gen b

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19

Ferguson, Shaun, Nikolaj B. Abel, Dugald Reid, et al. "A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes." PLOS ONE 18, no. 11 (2023): e0291680. http://dx.doi.org/10.1371/journal.pone.0291680.

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For decades, Agrobacterium rhizogenes (now Rhizobium rhizogenes), the causative agent of hairy root disease, has been harnessed as an interkingdom DNA delivery tool for generating transgenic hairy roots on a wide variety of plants. One of the strategies involves the construction of transconjugant R. rhizogenes by transferring gene(s) of interest into previously constructed R. rhizogenes pBR322 acceptor strains; little has been done, however, to improve upon this system since its implementation. We developed a simplified method utilising bi-parental mating in conjunction with effective counters

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20

Reddy, Gopal, Padma Shridhar, and H. Polasa. "Elimination of Col E1 (pBR322 and pBR329) plasmids inEscherichia coli on treatment with hexammine ruthenium(III) chloride." Current Microbiology 13, no. 5 (1986): 243–46. http://dx.doi.org/10.1007/bf01568646.

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21

Yakushevich, L. V., and L. A. Krasnobaeva. "Plasmid pBR322 and Nonlinear Conformational Distortions (Kinks)." Mathematical Biology and Bioinformatics 14, no. 1 (2019): 327–39. http://dx.doi.org/10.17537/2019.14.327.

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Plasmid pBR322 containing two coding regions in the matrix chain is a convenient object to study the DNA nonlinear dynamics that is known to play an important role in the processes of transcription, replication, denaturation and transmission of structural changes and information along the DNA molecule. The aim of the present work is to study by the methods of mathematical modeling the dynamics of local conformational distortions – kinks, in the plasmid pBR322. To calculate the dynamic characteristics of the kinks, we applied the method of McLaughlin-Scott, complemented by the block method. Thi

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22

WANG, TIAN P., JACQUES KAGAN, R. W. TUVESON та G. R. WANG. "α-TERTHIENYL PHOTOSENSITIZES DAMAGE TO pBR322 DNA". Photochemistry and Photobiology 53, № 4 (1991): 463–67. http://dx.doi.org/10.1111/j.1751-1097.1991.tb03657.x.

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23

Kagan, Jacques, Xinsheng Chen, Tian P. Wang, and P. Forlo. "PSORALENS CLEAVE pBR322 DNA UNDER ULTRAVIOLET RADIATION." Photochemistry and Photobiology 56, no. 2 (1992): 185–93. http://dx.doi.org/10.1111/j.1751-1097.1992.tb02146.x.

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24

Phillips, Steven J. "Recomblnant DNA in Wastewater: pBR322 Degradation Kinetics." Journal of Occupational and Environmental Medicine 32, no. 6 (1990): 497. http://dx.doi.org/10.1097/00043764-199006000-00003.

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25

Lodge, J. K., T. Kazic, and D. E. Berg. "Formation of supercoiling domains in plasmid pBR322." Journal of Bacteriology 171, no. 4 (1989): 2181–87. http://dx.doi.org/10.1128/jb.171.4.2181-2187.1989.

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26

Salgo, M. G., K. Stone, G. L. Squadrito, J. R. Battista, and W. A. Pryor. "Peroxynitrite Causes DNA Nicks in Plasmid pBR322." Biochemical and Biophysical Research Communications 210, no. 3 (1995): 1025–30. http://dx.doi.org/10.1006/bbrc.1995.1759.

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27

McNamara, P. T., I. Winicov, and R. E. Harrington. "Preferential nucleosome placement on pBR322 restriction fragments." Biochemical and Biophysical Research Communications 138, no. 1 (1986): 110–17. http://dx.doi.org/10.1016/0006-291x(86)90253-6.

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28

Fox, HB, P. De Togni, G. McMahon, et al. "Fate of the DNA in plasmid-containing Escherichia coli minicells ingested by human neutrophils." Blood 69, no. 5 (1987): 1394–400. http://dx.doi.org/10.1182/blood.v69.5.1394.1394.

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Abstract Escherichia coli minicells containing the plasmid pSC101 (approximately 10 kb) or pBR322 (approximately 4 kb) were opsonized and incubated with human neutrophils. The neutrophils responded to the minicells as they would to native E coli: they ingested the minicells, discharged their granule contents into the minicell-containing phagosomes, and expressed a respiratory burst. After one hour of incubation, the fate of the ingested plasmid DNA was examined. No DNA degradation was detected by trichloroacetic acid precipitation or agarose gel electrophoresis. Moreover, when pBR322 recovered

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29

Fox, HB, P. De Togni, G. McMahon, et al. "Fate of the DNA in plasmid-containing Escherichia coli minicells ingested by human neutrophils." Blood 69, no. 5 (1987): 1394–400. http://dx.doi.org/10.1182/blood.v69.5.1394.bloodjournal6951394.

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Escherichia coli minicells containing the plasmid pSC101 (approximately 10 kb) or pBR322 (approximately 4 kb) were opsonized and incubated with human neutrophils. The neutrophils responded to the minicells as they would to native E coli: they ingested the minicells, discharged their granule contents into the minicell-containing phagosomes, and expressed a respiratory burst. After one hour of incubation, the fate of the ingested plasmid DNA was examined. No DNA degradation was detected by trichloroacetic acid precipitation or agarose gel electrophoresis. Moreover, when pBR322 recovered from ing

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30

Solomko, A. P., A. V. Ryndich, T. G. Titok, et al. "The transgenic mice containing pATV-8 AND pBR322 plasmids." Biopolymers and Cell 4, no. 5 (1988): 267–69. http://dx.doi.org/10.7124/bc.000236.

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31

Legerski, R. J., J. E. Penkala, C. A. Peterson, and D. A. Wright. "Repair of UV-induced lesions in Xenopus laevis oocytes." Molecular and Cellular Biology 7, no. 12 (1987): 4317–23. http://dx.doi.org/10.1128/mcb.7.12.4317-4323.1987.

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We characterized a DNA repair system in frog oocytes by comicroinjection of UV-irradiated pBR322 DNA and radiolabeled nucleotides. Repair synthesis was monitored by incorporation of label into recovered pBR322 DNA and by a novel method in which the removal of UV photoproducts was determined from the shift of DNA topoisomers that occurs during gel electrophoresis upon repair of these lesions. We investigated the effects of several drugs in the oocyte system and found that although novobiocin, an inhibitor of topoisomerase II, was an effective inhibitor of repair, VM-26, another inhibitor of top

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32

Legerski, R. J., J. E. Penkala, C. A. Peterson, and D. A. Wright. "Repair of UV-induced lesions in Xenopus laevis oocytes." Molecular and Cellular Biology 7, no. 12 (1987): 4317–23. http://dx.doi.org/10.1128/mcb.7.12.4317.

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We characterized a DNA repair system in frog oocytes by comicroinjection of UV-irradiated pBR322 DNA and radiolabeled nucleotides. Repair synthesis was monitored by incorporation of label into recovered pBR322 DNA and by a novel method in which the removal of UV photoproducts was determined from the shift of DNA topoisomers that occurs during gel electrophoresis upon repair of these lesions. We investigated the effects of several drugs in the oocyte system and found that although novobiocin, an inhibitor of topoisomerase II, was an effective inhibitor of repair, VM-26, another inhibitor of top

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33

Lakshmi, V. V., P. Sridhar, B. T. Khan, and H. Polasa. "Mixed-Ligand Complexes of Platinum(II) as Curing Agents for pBR322 and pBR329 (ColE1) Plasmids in Escherichia coli." Microbiology 134, no. 7 (1988): 1977–81. http://dx.doi.org/10.1099/00221287-134-7-1977.

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34

Clark, Leann, Isabel Martinez-Argudo, Tom J. Humphrey, and Mark A. Jepson. "GFP plasmid-induced defects in Salmonella invasion depend on plasmid architecture, not protein expression." Microbiology 155, no. 2 (2009): 461–67. http://dx.doi.org/10.1099/mic.0.025700-0.

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We have investigated the impact of plasmids and GFP expression on invasion of cultured epithelial cells by Salmonella enterica Typhimurium strain SL1344. The invasiveness of SL1344 carrying plasmids derived from pBR322, encoding promoterless GFP or constitutively expressed rpsM-GFP, was compared under optimal growth conditions with that of SL1344(pBR322), unmodified SL1344 and a strain with chromosome-integrated rpsM-GFP. The strain carrying pBR322 exhibited normal invasion, but the presence of modified plasmids impaired invasiveness, and impairment was exacerbated by plasmid-encoded chloramph

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35

Moreno, Virtudes, Julia Lorenzo, Francesc X. Aviles, et al. "Studies of the Antiproliferative Activity of Ruthenium (II) Cyclopentadienyl-Derived Complexes with Nitrogen Coordinated Ligands." Bioinorganic Chemistry and Applications 2010 (2010): 1–11. http://dx.doi.org/10.1155/2010/936834.

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Four cationic ruthenium(II) complexes with the formula[Ru(η5-C5H5)(PPh3)2]+, withL=5-phenyl-1H-tetrazole (TzH)1, imidazole (ImH)2, benzo[1,2-b;4,3-b′] dithio-phen-2-carbonitrile (Bzt)3, and [5-(2-thiophen-2-yl)-vinyl]-thiophene-2-carbonitrile] (Tvt)4were prepared and characterized in view to evaluate their potentialities as antitumor agents. Studies by Circular Dichroism indicated changes in the secondary structure of ct-DNA. Changes in the tertiary structure of pBR322 plasmid DNA were also observed in gel electrophoresis experiment and the images obtained by atomic force microscopy (AFM) sugg

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36

Tabassum, Tahsin, Tasmin Tabassum, Nafisa Tabassum, Syeda Muntaka Maniha, and Rashed Noor. "Stability of plasmid pBR322 within Escherichia coli cells." MOJ Biology and Medicine 6, no. 1 (2021): 17–19. http://dx.doi.org/10.15406/mojbm.2021.06.00123.

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nsertion of plasmids into the bacterial cells is of great significance especially in course of the transfer of drug resistance, virulence and other traits. Retention of plasmids within the host bacteria is therefore an important factor for bacterial homeostasis. Current study inferred the pBR322 plasmid stability within the Escherichia coli competent cells. The calcium chloride heat shock method was used for the transformation purpose. The plasmid retention phenomenon was assessed through the replica plating. The results positively showed the plasmid retention within E. coli.

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37

Forte, Piero, Luisa Leoni, Beatrice Sampaolese, and Maria Savino. "Cooperativity in nucleosomes assembly on supercoiled pBR322 DNA." Nucleic Acids Research 17, no. 21 (1989): 8683–94. http://dx.doi.org/10.1093/nar/17.21.8683.

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Sutcliffe, J. "pBR322 and the advent of rapid DNA sequencing." Trends in Biochemical Sciences 20, no. 2 (1995): 87–90. http://dx.doi.org/10.1016/s0968-0004(00)88965-3.

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Tully, Douglas B., and John A. Cidlowski. "pBR322 contains glucocorticoid regulatory element dna consensus sequences." Biochemical and Biophysical Research Communications 144, no. 1 (1987): 1–10. http://dx.doi.org/10.1016/s0006-291x(87)80467-9.

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40

Topal, Michael D., Randy J. Thresher, Michael Conrad, and Jack Griffith. "NaeI endonuclease binding to pBR322 DNA induces looping." Biochemistry 30, no. 7 (1991): 2006–10. http://dx.doi.org/10.1021/bi00221a038.

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41

Schnaith, Leah M. T., Richard S. Hanson, and Lawrence Que. "Preferential hydrolysis of pBR322 plasmid by diiron complexes." Journal of Inorganic Biochemistry 51, no. 1-2 (1993): 525. http://dx.doi.org/10.1016/0162-0134(93)85551-i.

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42

Worthey, A. L., J. F. Kane, and D. R. Orvos. "Fate of pBR322 DNA in a wastewater matrix." Journal of Industrial Microbiology and Biotechnology 22, no. 3 (1999): 164–66. http://dx.doi.org/10.1038/sj.jim.2900629.

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43

Chiang, C. S., and H. Bremer. "Maintenance of pBR322-derived plasmids without functional RNAI." Plasmid 26, no. 3 (1991): 186–200. http://dx.doi.org/10.1016/0147-619x(91)90042-u.

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Morehouse, S. M., J. Cronin, L. Gomba, C. .Cooper, and G. Perna. "Interaction of Copper (II) complexes with pBR322 DNA." Journal of Inorganic Biochemistry 43, no. 2-3 (1991): 451. http://dx.doi.org/10.1016/0162-0134(91)84433-a.

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45

Zhang, Weiping, Emil M. Berberov, Jessica Freeling, D. He, Rodney A. Moxley, and David H. Francis. "Significance of Heat-Stable and Heat-Labile Enterotoxins in Porcine Colibacillosis in an Additive Model for Pathogenicity Studies." Infection and Immunity 74, no. 6 (2006): 3107–14. http://dx.doi.org/10.1128/iai.01338-05.

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ABSTRACT Although heat-stable (ST) and heat-labile (LT) enterotoxins produced by enterotoxigenic Escherichia coli (ETEC) have been documented as important factors associated with diarrheal diseases, investigations assessing the contributions of individual enterotoxins to the pathogenesis of E. coli infection have been limited. To address the individual roles of enterotoxins in the diarrheal disease caused by K88-positive ETEC in young pigs, enterotoxin-positive and -negative isogenic E. coli strains were constructed by using pBR322 to clone and express LT and STb. Four strains, K88+ astA, K88+

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46

S.Sreeremya. "Caryophanon latum." Research and Advances in Pharmacy and Life Sciences 1, no. 1 (2018): 18–22. https://doi.org/10.5281/zenodo.1921301.

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Caryophanon latum is a unique microorganism, which are microaerophilic, which hae a recognition site in an ideal bacterial vector PBR322.Majorly Caryophanon latum is isolated from the cow dung, these paper provides information on techniques implemented on isolation and culturing Caryophanon latum.

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Peterson, D. O., K. K. Beifuss, and K. L. Morley. "Context-dependent gene expression: cis-acting negative effects of specific procaryotic plasmid sequences on eucaryotic genes." Molecular and Cellular Biology 7, no. 4 (1987): 1563–67. http://dx.doi.org/10.1128/mcb.7.4.1563-1567.1987.

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A sequence element within pBR322 DNA mediates a cis-acting negative effect on expression from eucaryotic genes in transient expression assays. The negative element overlaps with sequences that inhibit DNA replication, but its effect is observed in the absence of detectable replication of transfected DNA.

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Peterson, D. O., K. K. Beifuss, and K. L. Morley. "Context-dependent gene expression: cis-acting negative effects of specific procaryotic plasmid sequences on eucaryotic genes." Molecular and Cellular Biology 7, no. 4 (1987): 1563–67. http://dx.doi.org/10.1128/mcb.7.4.1563.

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A sequence element within pBR322 DNA mediates a cis-acting negative effect on expression from eucaryotic genes in transient expression assays. The negative element overlaps with sequences that inhibit DNA replication, but its effect is observed in the absence of detectable replication of transfected DNA.

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Aceto, Serena, Antimo Di Maro, Barbara Conforto, et al. "Nicking activity on pBR322 DNA of ribosome inactivating proteins from Phytolacca dioica L. leaves." Biological Chemistry 386, no. 4 (2005): 307–17. http://dx.doi.org/10.1515/bc.2005.037.

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Abstract Ribosome-inactivating proteins isolated from Phytolacca dioica L. leaves are rRNA-N-glycosidases, as well as adenine polynucleotide glycosylases. Here we report that some of them cleave supercoiled pBR322 dsDNA, generating relaxed and linear molecules. PD-L1, the glycosylated major form isolated from the winter leaves of adult P. dioica plants, produces both free 3′-OH and 5′-P termini randomly distributed along the DNA molecule, as suggested by labelling experiments with [α-32P]dCTP and [γ-32P]dATP. Moreover, when the reaction is carried out under low-salt conditions, cleavage is obs

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Conter, Annie. "Plasmid DNA Supercoiling and Survival in Long-Term Cultures of Escherichia coli: Role of NaCl." Journal of Bacteriology 185, no. 17 (2003): 5324–27. http://dx.doi.org/10.1128/jb.185.17.5324-5327.2003.

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ABSTRACT The relationship between the survival of Escherichia coli during long-term starvation in rich medium and the supercoiling of a reporter plasmid (pBR322) has been studied. In aerated continuously shaken cultures, E. coli lost the ability to form colonies earlier in rich NaCl-free Luria-Bertani medium than in NaCl-containing medium, and the negative supercoiling of plasmid pBR322 declined more rapidly in the absence of NaCl. Addition of NaCl at the 24th hour restored both viability and negative supercoiling in proportion to the concentration of added NaCl. Addition of ofloxacin, a quino

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