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Rozprawy doktorskie na temat „PCR-RFLP”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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Šuranská, Hana. "Identifikace vinných kvasinek metodou PCR-RFLP." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216494.

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This thesis deals with identification of the wine yeasts by applying the PCR-RFLP method. The identification and characteristic of the yeasts has gone through substantial changes in recent years. There have been introduced new methods of taxonomic classifying based on the molecular methods, which are oriented to easy and fast identification. One of these methods is the PCR-RFLP method. The amplification of the 5•8S-ITS rDNA sequence by the polymerase chain reaction with use of the primers ITS1 and ITS4 leads to the amplification of the specific sequence of DNA. Such multiplied DNA is after rep

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Ziebell, Kim. "Evaluation of PCR and PCR-RFLP protocols to identify the Shiga toxins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0026/MQ51107.pdf.

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Olivová, Radana. "Optimalizace metody PCR-RFLP pro taxonomické zařazení kvasinek." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216560.

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This thesis deal with optimalization method PCR – RFLP for taxonomy enlistment of yeasts. Conventional identification methods for yeasts are time-consuming. Molecular biological method based on PCR are instrumental towards fast and precise identification as compared to conventional phenotypic methods. In this thesis molecular biological method PCR – RFLP was used for identification and enlistment of yeasts. This metod follow repeating spacers of ribozomal DNA of yeast, characteristic for each species and strain. By the help of PCR were amplified specific partitions of DNA. These fragments of D

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Arraes, Ana Carolina Palmeira. "Detecção da diversidade molecular de Candida spp. isoladas de UTI neonatal." reponame:Repositório Institucional da UFBA, 2013. http://www.repositorio.ufba.br/ri/handle/ri/11817.

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Submitted by Hiolanda Rêgo (hiolandar@gmail.com) on 2013-06-10T20:55:35Z No. of bitstreams: 1 Dissertação_ICS_ Ana Carolina Arraes.pdf: 1876812 bytes, checksum: c4740a70b3675be50d2ebafeb0ba8fe3 (MD5)<br>Made available in DSpace on 2013-06-10T20:55:35Z (GMT). No. of bitstreams: 1 Dissertação_ICS_ Ana Carolina Arraes.pdf: 1876812 bytes, checksum: c4740a70b3675be50d2ebafeb0ba8fe3 (MD5)<br>CAPES<br>A incidência de candidemia tem aumentado nos últimos anos e o espectro das espécies de Candida tem mudado com a emergência das espécies não-albicans e com o aumento da resistência antifúngica. A técn

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Malvárez, Macedo Gabriela. "Aplicação das metodologias PCR/RFLP para caracterização de fungos ectomicorrízicos em eucalipto /." reponame:Repositório Institucional da UFSC, 1999. https://repositorio.ufsc.br/handle/123456789/112290.

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MEDEIROS, Rafael Acioli. "Caracterização da Leishmania infantum e Leishmania (Viannia) braziliensis em cães provenientes da Região Metropolitana do Recife, Pernambuco." Universidade Federal de Pernambuco, 2013. https://repositorio.ufpe.br/handle/123456789/12218.

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Submitted by Milena Dias (milena.dias@ufpe.br) on 2015-03-12T17:45:21Z No. of bitstreams: 2 Dissertação Rafael Medeiros.pdf: 841724 bytes, checksum: 770fac09975639de3352fde1d0ea2ec3 (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5)<br>Made available in DSpace on 2015-03-12T17:45:21Z (GMT). No. of bitstreams: 2 Dissertação Rafael Medeiros.pdf: 841724 bytes, checksum: 770fac09975639de3352fde1d0ea2ec3 (MD5) license_rdf: 1232 bytes, checksum: 66e71c371cc565284e70f40736c94386 (MD5) Previous issue date: 2013<br>CAPES<br>As Leishmanioses são uma antropozoonose co

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Björkbom, Tommy. "PCR-RFLP analys av mt-DNA hos Öring (Salmo trutta) i Gävleborgs län." Thesis, University of Gävle, Department of Electronics, Mathematics and Natural Sciences, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-7624.

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Sultan, Amal Hassan. "The development of molecular tools for identification of Leishmania species by PCR-RFLP." Thesis, University of Liverpool, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.479067.

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Abreu, Daniel Paiva Barros de. "Caracteriza??o fenot?pica, genot?pica e perfil de sensibilidade a antif?ngicos de isolados cl?nicos de c?es e gatos pertencentes ao Complexo Sporothrix schenckii oriundos do estado do Rio de Janeiro." Universidade Federal Rural do Rio de Janeiro, 2017. https://tede.ufrrj.br/jspui/handle/jspui/2031.

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Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-09-13T12:31:48Z No. of bitstreams: 1 2017 - Daniel Paiva Barros de Abreu.pdf: 1307092 bytes, checksum: b384d4db2cb316d3f640b111bc8efba4 (MD5)<br>Made available in DSpace on 2017-09-13T12:31:48Z (GMT). No. of bitstreams: 1 2017 - Daniel Paiva Barros de Abreu.pdf: 1307092 bytes, checksum: b384d4db2cb316d3f640b111bc8efba4 (MD5) Previous issue date: 2017-02-20<br>Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico - CNPq<br>Dimorphic fungi belonging to Sporothrix schenckii complex are responsible for sporotrichosis, importa

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Dlapalová, Kristýna. "Izolace a charakterizace autochtonních kvasinek z interspecifické odrůdy vinné révy." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217112.

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The aim of this thesis is the isolation and identification of yeasts obtained from the wine berries and the characterization of the collection yeast by using processes of PCR - RFLP. The type yeasts were obtained from the collection of yeasts of CCY in Bratislava, yeasts from the wine berries were collected from the species of Hibernal wine from the wineries of Štěpán Maňák. Identification of individual yeast is then based on analysis of the DNA segment in the area of 5,8S - ITS using primers ITS1 and ITS4. The restriction analysis was performed using restriction endonucleases HaeIII, HinfI, H

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Spengler, C. J. "PCR-RFLP typification of microbes used in the production of a fermented fish product." Thesis, Stellenbosch : Stellenbosch University, 2001. http://hdl.handle.net/10019.1/52397.

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Thesis (MScFoodSc)--Stellenbosch University, 2001.<br>ENGLISH ABSTRACT: The preservation of various fresh fish products is achieved by either smoking, salting, canning, freezing or fermenting a highly perishable raw product. Since many of these facilities are not readily available, the use of fermentation as a means of preserving the product has been extensively practiced. However, the fermentation of fish is a time consuming practise and only by accelerating the process would it be possible to ensure the production of a more cost effective and readily available safe end-product. The q

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Okuda, Liria Hiromi. "Orthopoxvirus bovino: inquérito soroepidemiológico e caracterização de amostras pela técnica de PCR e RFLP." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-09122013-152339/.

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No Brasil, casos de doença exantemática em bovinos e humanos têm sido relatados em diversas regiões, cujo agente causal é o virus vaccinia pertencente ao gênero Orthopoxirus da família Poxviridae. Classicamente, a varíola bovina é causada pelo Cowpoxvirus, entretanto, outros Poxvirus, como o vírus vaccinia podem desenvolver sintomatologia clínica semelhante. Além de comprometer a cadeia produtiva de leite, uma vez que dificulta a ordenha, predispõe a mastite e descarte do produto, é uma zoonose e atualmente está incluída no diagnóstico de doença vesicular. A origem desses casos bem como a epid

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Alvarez, Vega Luis Guillermo. "Detección de Staphylococcus pseudintermedius y Staphylococcus aureus aislados de piodermias caninas mediante PCR-RFLP." Bachelor's thesis, Universidad Nacional Mayor de San Marcos, 2019. https://hdl.handle.net/20.500.12672/11838.

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A nivel mundial la piodermia es una de las enfermedades de la piel más diagnosticada en caninos. Entre los agentes involucrados se encontraba Staphylococcus intermedius, el que se creía el principal agente de las piodermias caninas; sin embargo, en el año 2005 fue reclasificado en 3 especies: S. intermedius, S. pseudintermedius y S. delphini. Posteriormente, estudios en diferentes países reportaron que el Staphylococcus pseudintermedius es en realidad el agente bacteriano más frecuentemente aislado en piodermias, seguidamente surgieron los primeros reportes de aislados resistentes a met

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Georges, Agnès. "Chlamydia Trachomatis et tetracyclines : étude de souches récidivantes par PCR-RFLP du gène omp1." Bordeaux 2, 1994. http://www.theses.fr/1994BOR2P094.

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Pereira, Lucas Carlos Gomes. "Importância dos polimorfismos C3435T e C1236T do gene de resistência a múltiplas drogas (MDR1) na resposta ao tratamento com mesilato de imatinib em pacientes com Leucemia Mielóide Crônica (LMC)." Universidade Federal de Goiás, 2013. http://repositorio.bc.ufg.br/tede/handle/tede/3100.

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Submitted by Cássia Santos (cassia.bcufg@gmail.com) on 2014-09-19T12:13:58Z No. of bitstreams: 2 Dissertacao Lucas C G Pereira.pdf: 869540 bytes, checksum: 9c40d119515c83b8b52606c52be6703d (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)<br>Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2014-09-19T12:56:29Z (GMT) No. of bitstreams: 2 Dissertacao Lucas C G Pereira.pdf: 869540 bytes, checksum: 9c40d119515c83b8b52606c52be6703d (MD5) license_rdf: 23148 bytes, checksum: 9da0b6dfac957114c6a7714714b86306 (MD5)<br>Made available in DSpace on

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Vitaliano, Sérgio Netto. "Isolamento e caracterização biológica e genotípica de Toxoplasma gondii em animais selvagens do Brasil." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-04102012-182312/.

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Toxoplasma gondii é um protozoário formador de cistos capaz de infectar diversas espécies de aves e mamíferos domésticos e selvagens, incluindo o homem. Apesar de evidências sorológicas da infecção por T. gondii em animais selvagens, pouco se sabe sobre o papel da vida selvagem na cadeia epidemiológica e tampouco a susceptibilidade das variadas espécies a este parasito. O presente trabalho consistiu no isolamento e caracterização genotípica de T. gondii de tecidos de animais selvagens, de vida livre e de cativeiro, provenientes de diversas localidades do Brasil e na detecção sorológica de anti

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Andrade, Vanessa da Silva Silveira. "Polimorfismos genéticos, susceptibilidade e resposta ao tratamento em crianças portadoras de leucemia linfoblástica aguda." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/17/17135/tde-27082007-085802/.

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As leucemias constituem o câncer mais comum da infância, representando 30% de todas as neoplasias infantis. Dentre elas, a leucemia linfoblástica aguda (LLA) é a mais freqüente, atingindo 75% dos casos pediátricos de leucemias. A ocorrência da LLA tem sido relacionada com a exposição a alguns fatores ambientais (químico, físico e biológicos) e maternos (uso de drogas e dieta) tanto no desenvolvimento intra-útero como após o nascimento. No entanto, o processo de leucemogênese, particularmente com relação à importância da susceptibilidade genética herdade e fatores ambientais, ainda não foi eluc

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Fialová, Lenka. "Izolace a identifikace kvasinek z vinice pro jejich možné využití k výrobě vína." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240584.

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The aim of this work was to isolate the Saccharomyces cerevisiae yeast from the surface of wine grapes of the Malverina and Sauvignon varieties. Isolated yeasts were identified by the PCR-RFLP method. 5,8S ITS rDNA was amplified using PCR and restricition endonucleases HaeIII, HhaI, HinfI and TaqI were used for the restriction analysis which followed. The results were processed by UPGMA cluster analysis using the BioNumerics programme. The dominant genus on the surface of the Malverina variety grapes was Brettanomyces/Dekkera, while on the surface of the Sauvignon grapes we found mainly yeasts

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Michálek, Petr. "Sledování vlivu použité autochtonní kultury kvasinek na kvasný proces výroby vína." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2015. http://www.nusl.cz/ntk/nusl-217130.

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This thesis deals with the identification of wine yeasts isolated from the grape must using PCR-RFLP method. The yeasts were isolated from Pinot Noir grape variety must. Grapes were grown and produced in accordance with the requirements placed on organic and integrated farming. Samples were processed in the laboratory, where pure cultures of individual yeast were obtained. A commercial kit was used for yeast DNA isolation. Obtained DNA was used for further analysis. Using the polymerase chain reaction and the primers ITS1 and ITS4 a specific segment of 5.8S rDNA-ITS region was amplified. The P

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陳子明 and Tsz-ming Chan. "Comparison of molecular epidemiological study on Mycobacterium tuberculosis using IS6110 RFLP and IS6110 PCR typing." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2000. http://hub.hku.hk/bib/B31969689.

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Chan, Tsz-ming. "Comparison of molecular epidemiological study on Mycobacterium tuberculosis using IS6110 RFLP and IS6110 PCR typing." Hong Kong : University of Hong Kong, 2000. http://sunzi.lib.hku.hk/hkuto/record.jsp?B22029850.

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Esmerini, Patrícia de Oliveira. "Ocorrência e isolamento de Toxoplasma gondii e Neospora spp. em equídeos do Brasil." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-11062013-103048/.

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Neospora caninum é um parasito intracelular obrigatório, formador de cistos, que acomete vários animais domésticos e silvestres, tendo maior importância nas espécies canina e bovina, nas quais causa problemas nervosos e reprodutivos. Os canídeos do gênero Canis são os únicos reconhecidos como hospedeiros definitivos do N. caninum até o momento, nos quais ocorre a fase sexuada de multiplicação, resultando na eliminação de oocistos pelas fezes. Toxoplasma gondii também é um coccídio responsável por uma das zoonoses de maior importância e ocorrência em todo o mundo. A fase assexuada de desenvolvi

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Mazurowski, Artur. "Analiza wpływu polimorfizmu wybranych genów w odniesieniu do cech reprodukcyjnych loch ras polska biała zwisłoucha i wielka biała polska." Rozprawa doktorska, Uniwersytet Technologiczno-Przyrodniczy w Bydgoszczy, 2018. http://dlibra.utp.edu.pl/Content/1171.

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Celem pracy była identyfikacja polimorfizmu w odrębie genów: RYR1, AREG, EGF, SPP1, GH, GH-RH, PIT1, BF, ZAR1 oraz określenie ich struktury genetycznej w populacji loch ras polska biała zwisłoucha (pbz) i wielka biała polska (wbp), a także analiza wpływu polimorfizmu wybranych genów na cechy reprodukcyjne<br>The aim of the present was to identify polymorphism within the genes: RYR1, AREG, EGN, SPP1, GH, GH-RH, PIT1, BF, ZAR1 and to determine their genetic structure in two Polish sow breads: Polish Landrace and Polish Large White breeds. In addition the aim was to find associations between poly

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Škodová, Anna. "Identifikace kvasinek rodu Saccharomyces z listů, bobulí a moštu révy vinné." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2008. http://www.nusl.cz/ntk/nusl-216401.

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This diploma thesis is concerned about the PCR-RFLP method for identification of yeasts genus Saccharomyces from leaves, grapes and must of white wine. The thesis describes an obtaining of pure cultures of yeasts, isolation DNA and amplification of specific segments of DNA using PCR method. Splitting of this segments by restriction endonukleases and a detection of final fragments by zone electrophoresis are mentioned too. Length of fragments and numbers of restriction fragments, which are characteristic of every species, enable a determination of microorganisms and their inclusion into the sys

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Del, Valle Luis J., Michael L. Jaramillo, Miguel Talledo, et al. "Development of a 16S rRNA PCR-RFLP Assay for Bartonella Identification: Applicability in the Identification of Species Involved in Human Infections." Horizon Research Publishing, 2014. http://hdl.handle.net/10757/322342.

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Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic

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Krátká, Veronika. "Výběr vhodných autochtonních kvasinek pro výrobu vína." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2014. http://www.nusl.cz/ntk/nusl-217062.

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The aim of this diploma thesis was the selection of appropriate indigenous yeasts for wine production. Tested yeasts were isolated from the grapes from the winery Maňák Žádovice. The yeasts isolated within theses in 2009 – 2012 have been also tested, and commercial wine yeast have been tested for comparison. In the theoretical part the focus is on the technology of wine, in particular fermentation. The work is also focused on yeasts metabolism and taxonomy. There was described the principle of PCR-RFLP, and methods used to characterize the properties of isolated yeasts. In the experimental par

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Piza, Vanessa Mirabelli Toledo [UNESP]. "Detecção e diferenciação do vírus da bronquite infecciosa pela técnica de imunocaptura-RT-PCR E RFLP." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/94869.

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Made available in DSpace on 2014-06-11T19:27:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-07-06Bitstream added on 2014-06-13T20:16:37Z : No. of bitstreams: 1 piza_vmt_me_jabo.pdf: 496784 bytes, checksum: 8e82f3b02d746dfe3daa52fd23b8be9e (MD5)<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)<br>Nesse estudo foi desenvolvido e aplicado o procedimento de imunocaptura para realizar a reação de transcrição reversa seguida pela reação em cadeia da polimerase (IC-RT-PCR) a fim de ser amplificada uma região 5'_ proximal do gene 81 do vírus da bronquite infecciosa (

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Piza, Vanessa Mirabelli Toledo. "Detecção e diferenciação do vírus da bronquite infecciosa pela técnica de imunocaptura-RT-PCR E RFLP /." Jaboticabal : [s.n.], 2007. http://hdl.handle.net/11449/94869.

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Orientador: Hélio José Montassier<br>Banca: Liana Brentano<br>Banca: Maria da Glória Buzinaro<br>Resumo: Nesse estudo foi desenvolvido e aplicado o procedimento de imunocaptura para realizar a reação de transcrição reversa seguida pela reação em cadeia da polimerase (IC-RT-PCR) a fim de ser amplificada uma região 5'_ proximal do gene 81 do vírus da bronquite infecciosa (VBI) com 228 pb e de forma a fazer a detecção e a diferenciação desse vírus, comparando-se os resultados dessa metodologia com os obtidos na técnica convencional de RT-PCR. Todas as 11 estirpes do VBI testadas foram amplificada

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Trevisani, Natascha. "Genotipagem de isolados de Toxoplasma gondii obtidos de Gallus gallus naturalmente infectados no estado de Santa Catarina." Universidade do Estado de Santa Catarina, 2013. http://tede.udesc.br/handle/handle/878.

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Made available in DSpace on 2016-12-08T16:24:13Z (GMT). No. of bitstreams: 1 PGCA13MA101.pdf: 699148 bytes, checksum: 49c5f8f78753d18ac0c7b049d5052f86 (MD5) Previous issue date: 2013-02-21<br>Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>Toxoplasmosis is a widely-distributed zoonosis that can infect warm-blooded animals, including birds and humans. Chickens, as well as other birds, can be considered indicators of environmental contamination. Toxoplasma gondii has a distinct clonal populational structure of three lineages (I, II and III) with high recombination, resulting in

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Moreira, Abdiel Aparecido. ""Pesquisa de sítios de restrição enzimática em segmento da ORF K1 do genoma de herpesvírus humano tipo 8 (HHV-8) em isolados clínicos de São Paulo: relação com subtipos virais e implantação da técnica de RFLP (Restriction Fragment Length Polymorphism Analyses) para determinar subtipos virais"." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/9/9136/tde-01102004-164856/.

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A epidemia da Síndrome de Imunodeficiência Adquirida (AIDS) fez aumentar a incidência de sarcoma de Kaposi (SK) em todos os países e o SK passou a ser considerado doença definidora de AIDS. Desde a descoberta de seu agente etiológico, o herpesvírus humano tipo 8 (HHV-8), vários estudos vêm sendo realizados com o objetivo de caracterizar subtipos virais presentes em todas as formas de SK: clássica, endêmica, iatrogênica e epidêmica. Os sistemas rotineiramente usados na subtipagem do HHV-8 têm usado o seqüenciamento de um gene que contém regiões hipervariáveis e que codifica uma glicoproteína de

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Contieri, Marcelo Bittencourt. "Estudo comparativo entre os métodos genético e morfológico para a identificação entre espécies, utilizando o tecido ósseo como matéria." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/10/10133/tde-26012007-142544/.

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Na medicina legal a identificação de produtos de origem animal vem atingindo grande importância. A grande maioria das amostras encontradas na cena do crime são fragmentos de tecido não facilmente degradável, entre estes o tecido ósseo. Por ter sua estrutura composta por uma matriz mineral o osso preserva sua característica morfológica e material genético viável. Os objetivos do presente trabalho foram: caracterizar morfologicamente o tecido íntegro, agregando assim mais informações a respeito da espécie animal, e verificar se os dados da literatura quanto à identificação genética são confiávei

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Paulino, Rosangela Clara. "Detecção molecular de Giardia sp em amostras fecais e água : extração de DNA genomico, PCR e RFLP /." oai:ufpr.br:234179, 2005. http://200.17.209.5:8000/cgi-bin/gw_42_13/chameleon.42.13a?host=localhost%201111%20DEFAULT&sessionid=VTLS&function=CARDSCR&search=KEYWORD&pos=1&u1=12101&t1=234179.

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Orientadora : Vanete Thomaz Soccol<br>Tese (doutorado) - Universidade Federal do Paraná, Setor de Tecnologia, Programa de Pós-Graduaçao em Processos Biotecnológicos. Defesa: Curitiba, 2005<br>Inclui bibliografia<br>Área de concentração: Saude humana e animal

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Frota, Maria Zeli Moreira, and 92-98231-9393. "Avaliação de métodos de extração de DNA e de identificação de dermatófitos por análise de PCR-RFLP." Universidade Federal do Amazonas, 2011. http://tede.ufam.edu.br/handle/tede/5883.

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May, Duncan Robert. "Population genetics of Penaeus vannamei on the West Coast of Mexico." Thesis, Bangor University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312405.

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Markowska, Agnieszka Magdalena. "Polimorfizm genu białka prionowego owiec w kodonach: 127, 141, 151, 171 i 198." Rozprawa doktorska, [Nakł.aut.], 2013. http://dlibra.utp.edu.pl/Content/561.

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Celem pracy było zbadanie polimorfizmu genu PRNP w kodonach: 127, 141, 151, 171 i 189 owiec (n=424) pochodzących z Polski, Słowacji i Włoch. Oszacowano frekwencje alleli i genotypów oraz porównano badane populacje owiec pod względem zmienności w tym zakresie

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FREITAS, L. B. "Caracterização Molecular de Papilomavírus Humano (HPV) e Vírus Adeno-Associado (AAV) em Lesões Intraepiteliais de Colo Uterino: Um Estudo de Seguimento." Universidade Federal do Espírito Santo, 2014. http://repositorio.ufes.br/handle/10/4597.

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Made available in DSpace on 2016-08-29T15:35:05Z (GMT). No. of bitstreams: 1 tese_7779_TESE LBF-2014.pdf: 10712510 bytes, checksum: 8a20db56f2fe6ca735be70a109f4ef61 (MD5) Previous issue date: 2014-06-04<br>O câncer de colo uterino (CCU), cujo agente etiológico é o papilomavírus humano (HPV), é um dos tipos de câncer mais frequentes em mulheres em todo o mundo, não só em incidência como também em mortalidade. Alguns genótipos de HPV, denominados de alto risco (HR-HPV), e suas variantes gênicas, estão mais associados à indução de lesões malignas, sendo HPV16 e 18 os mais frequentes. Algumas in

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Šerý, Filip. "Sledování vlivu použité komerční kultury kvasinek na kvasný proces výroby vína." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2013. http://www.nusl.cz/ntk/nusl-216920.

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This diploma thesis focuses on isolation and taxonomic classification of yeast species isolated during the red wine (Pinot noir) fermentation. Grapes were grown under organic and integrated farming in South Moravia wine region, Czech Republic. Processing was controlled – for inoculation was used strain Saccharomyces cerevisiae BS6. Polymerase chain reaction followed by restriction fragment lenght polymorphism of PCR-amplified fragments (PCR-RFLP) was used for yeast species identification. For DNA analysis we used coding region of 5.8S ITS rDNA which was amplified using ITS1-ITS4 primers. Ampli

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Cabral, Cynthia Hatsue Kitayama. "Determina??o de hapl?tipos do gene ?s em portadores de anemia falciforme." Universidade Federal do Rio Grande do Norte, 2010. http://repositorio.ufrn.br:8080/jspui/handle/123456789/18543.

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Made available in DSpace on 2015-03-03T14:03:52Z (GMT). No. of bitstreams: 1 CynthiaHKC_DISSERT.pdf: 2413327 bytes, checksum: 062d4378968e15e34eed7123a46717b9 (MD5) Previous issue date: 2010-05-11<br>Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico<br>Haplotypes linked to the ?S gene represent patterns of DNA polymorphisms along chromosome 11 of individuals bearing the ?S gene. Analysis of haplotypes, in addition to serving as an important source for anthropological studies about the ethnic origin of a population, contributes to a better understanding of the variations in c

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Paiva, Samuel Rezende. "Caracterização da diversidade genética de ovinos no Brasil com quatro técnicas moleculares." Universidade Federal de Viçosa, 2005. http://www.locus.ufv.br/handle/123456789/10554.

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Submitted by Marco Antônio de Ramos Chagas (mchagas@ufv.br) on 2017-06-06T11:18:38Z No. of bitstreams: 1 texto completo.pdf: 909512 bytes, checksum: 95d360795ba1a42ae10b3949c99caca0 (MD5)<br>Made available in DSpace on 2017-06-06T11:18:38Z (GMT). No. of bitstreams: 1 texto completo.pdf: 909512 bytes, checksum: 95d360795ba1a42ae10b3949c99caca0 (MD5) Previous issue date: 2005-12-14<br>Fundação de Amparo a Pesquisa do Estado de Minas Gerais<br>Os ovinos foram introduzidos no Brasil principalmente pelos portugueses e espanhóis durante o processo de colonização. Por motivos sócio-culturais, a

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Sadel, Peter. "Identifikace kvasinek z interspecifické odrůdy vinné révy." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240551.

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The main goal of my diploma thesis was to identify and characterize yeasts from must Hibernal and also collection yeasts by using methods called RFLP-PCR (Restriction Fragment Length Polymorphism - Polymerase Chain Reaction). Theory was the first part of my diploma thesis which dealt with wine, yeasts and molecular methods. Theory section was followed by experimental section divided into two parts. The main goal of the first part was to characterize and identify yeasts from must Hibernal by using PCR and RFLP-PCR methods. In the samples there were found yeasts Saccharomyces and Pichia. The sec

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41

Masoudi, Mehrnoush. "Identification of variants within the coding region and 5'-flanking region of the k-casein encoding gene in Holsteins using PCR-RFLP and PCR-SSCP analyses." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23409.

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Single-strand conformation polymorphism analysis (SSCP) and restriction fragment length polymorphism analysis (RFLP) were used to determine the genotype of Holsteins at the $ kappa$-casein ($ kappa$-CN) locus. A 432-bp fragment within exon IV containing nucleotide substitutions diagnostic of the A- and B-variants of $ kappa$-CN was amplified using the polymerase chain reaction (PCR). The sires from the earliest years of the AI industry had a significantly higher (p $<$ 0.01) frequency of allele than sires in modern usage. These data indicate that selection or milk production parameters may dis

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42

Mohamed, Ahmed Abdel-Rady Mahmoud. "Bestimmung der Wirtstierarten in Blutmahlzeiten von Tsetsefliegen (Diptera: Glossinidae) mittels der Polymerasekettenreaktion und Restriktionsfragment-Längenpolymorphismus- Analyse (PCR-RFLP)." Berlin : Mensch-und-Buch-Verl, 2004. http://www.diss.fu-berlin.de/2004/175/index.html.

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Corcelle, Benjamin. "Mise au point et évaluation d'un test pour le diagnostic des hémoglobinoses S et C par PCR-RFLP." Nantes, 2001. http://www.theses.fr/2001NANT012P.

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Schlögl, Paulo Sérgio. "Análise da diversidade genética em regiões não codificadoras de DNAs de cloroplastos em araucaria angustifolia por PCR-RFLP." Florianópolis, SC, 2000. http://repositorio.ufsc.br/xmlui/handle/123456789/79098.

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Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas.<br>Made available in DSpace on 2012-10-17T23:11:38Z (GMT). No. of bitstreams: 0Bitstream added on 2014-09-25T17:02:36Z : No. of bitstreams: 1 170181.pdf: 4522961 bytes, checksum: 7850488cbaae2f803b9149bd8813629b (MD5)<br>Caracteriza a variabilidade genética em regiões não codificadoras dos genomas de cloroplasto de Araucaria angustifolia, em populações naturais do Estado de Santa Catarina, utilizando a reação de PCR seguida por RFLP. Estuda como é distribuida a diversidade nas populações da espécie,

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45

Arnaldo, Marcela. "Relações entre fluxos de óxido nitroso (N2O) com umidade e genes associados à desnitrificação em floresta e sistemas agrícolas." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-05112014-152017/.

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O óxido nitroso (N2O) é um importante gás de efeito estufa (GEE) e, nos ecossistemas terrestres, é produzido principalmente pelo processo de desnitrificação. Esse ocorre em condições anaeróbias e, portanto, é fortemente estimulado pelo aumento do teor de umidade do solo. Entretanto, solos sob diferentes usos podem exibir taxas de emissão de N2O distintas, mesmo quando apresentam teores de umidade equivalentes. Ainda não está claro se isso se deve somente ao fato de os mesmos diferirem quanto a atributos físicos e químicos capazes de afetar a atividade dos organismos desnitrificantes ou se tamb

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46

Ayala, Claudia de Oliveira. "Sorologia de antígenos flagelares de amostras de Escherichia coli Enteropatogênicas (EPEC) e E. coli produtoras da Toxina de Shiga (STEC) isoladas de diferentes animais e análise comparativa do gene fliC por PCR-RFLP." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-02022010-100052/.

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A espécie Escherichia coli constitui um grupo de bactérias tipicamente não patogênicas e que fazem parte do trato intestinal de humanos e animais. As amostras são sorotipadas com base em seus antígenos de superfície O (somático), H (flagelar) e K (capsular). O antígeno flagelar correspondente ao filamento é formado pela polimerização da flagelina, codificada pelo gene fliC. O presente trabalho empregou a técnica de PCR-RFLP para analisar os padrões de antígenos flagelares de 112 amostras de EPEC e STEC. Quatorze amostras não amplificaram o gene fliC, 17 tiveram seu antígeno flagelar determinad

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47

Beníčková, Romana. "Sledování vlivu použití autochtonní kvasinky při výrobě vína v podmínkách vinařství." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2014. http://www.nusl.cz/ntk/nusl-217061.

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This thesis deals with identification of yeasts by applying the RFLP-PCR method. Objective of the thesis was to identify the yeasts present in wine from Grüner Veltliner during fermentation. Identification was made by amplification of 5,8S-ITS sequences of DNA by the polymerase chain reaction with primers ITS1 and ITS4. Amplified DNA was submitted to the restriction analysis by restriction endonuclease HaeIII, HinfI and HhaI. By restriction analysis with a specific enzyme, the amplified DNA is chopped into the specific fragments which are characteristic for given kind of yeasts. In the analyse

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48

Augustová, Kamila. "Taxonomické zařazení kvasinek rodu Saccharomyces." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2011. http://www.nusl.cz/ntk/nusl-216792.

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The theoretical part discusses the yeasts and their taxonomic classification using traditional methods and using modern methods. Detail the work is concerned with descriptions of modern molecular-biology methods. The practical part was analyzed DNA by PCR-fingerprinting (rep-PCR) type of yeasts, which we received from the CCY and subsequent analysis of yeast samples obtained from grape musts. One of the grape must was obtained in 2009 (white grape variety) and the second in 2010 (red grape variety). Both grape musts come as integrated vineyards and organic. Grape musts samples were obtained fr

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Petrášová, Ludmila. "Sledování vlivu kvasinek na chemické a senzorické vlastnosti vyráběného vína." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2016. http://www.nusl.cz/ntk/nusl-240527.

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The thesis deals with monitoring of the yeast's impact to the chemical and sensorial characteristics of produced wine. The aim of experimental part was obtaining of the aroma-active substances in the fermenting must and wine Hibernal and wine Pinot Blanc. SPME-GC-MS methods were used for identify wines and must aroma. The same autochthonous yeasts were used for wine production. This yeast was isolated from surface of grapes in our laboratory. It was found that both wines have a similar aromatic profile. The next aim was the monitoring of chemical parameters of samples and their changes. For th

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Sanches, Letícia da Cruz [UNESP]. "Caracterização das espécies de leishmania em sangue periférico de cães por PCR-RFLP NA área endêmica de Bauru/SP." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/128128.

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Made available in DSpace on 2015-10-06T13:03:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-06-16. Added 1 bitstream(s) on 2015-10-06T13:18:30Z : No. of bitstreams: 1 000849239.pdf: 306516 bytes, checksum: cab162ea96c4456d9e47531798b2c3eb (MD5)<br>Leishmaniasis is a major public health problem in the world. The protozoa of the genus Leishmania has worldwide distribution and epidemiology of the disease depends on the characteristics of the parasites. Leishmaniasis are divided into visceral leishmaniasis and cutaneous. The dog plays a key role in the transmission of L. infantum to

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