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Rozprawy doktorskie na temat „Polymerase chain reaction”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Feugeas, Olivier. "Pcr (polymerase chain reaction) et vih." Lille 2, 1990. http://www.theses.fr/1990LIL2M264.

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Lantz, Pär-G. "PCR-based detection of microorganisms in complex biological samples." Lund : Dept. of Applied Microbiology, Lund University, 1998. http://catalog.hathitrust.org/api/volumes/oclc/39178906.html.

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Verhaegen, Monique Elise. "Novel approaches in quantitative polymerase chain reaction." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape10/PQDD_0021/MQ52489.pdf.

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4

Chiou, Jeffrey Tsungshuan. "A novel capillary polymerase chain reaction machine." Thesis, Massachusetts Institute of Technology, 2001. http://hdl.handle.net/1721.1/8864.

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Thesis (Ph.D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2001.<br>Includes bibliographical references (p. 254-268).<br>I built a novel prototype capillary polymerase chain reaction machine. The purpose was to perform a single reaction as fast as possible with a reaction volume - 100 nl. The PCR mix is in the form of a 1 /1 droplet that moves between three heat zones inside of a 1 mm I.D. capillary filled with mineral oil via pneumatic actuation. A laser beam waveguides down the capillary until it strikes the drop, at which point it scatters. The scatter is picked

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5

Clackson, Timothy Piers. "Antibody engineering using the polymerase chain reaction." Thesis, University of Cambridge, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.316695.

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6

Linley, M. "The detection of polymerase inhibiting lesions using the polymerase arrest polymerase chain reaction assay." Thesis, Swansea University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.637924.

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There is a constant need to determine the genotoxic potential of the agents to which the human population is exposed. The stringent testing of new products is legislatively controlled and dependent on the accumulation of sufficient scientific data to allow an analysis of the risk. It is important to predetermine any risks in the workplace prior to the presentation of disease and to provide factual public information on personal exposure e.g. the risks associated with UV light. Various experimental assays have been developed to assess the genotoxicity, mutagenicity and mcarcinogenicity of given

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Nebbali, M. "Human gene mapping using the polymerase chain reaction." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317395.

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Borges, Pinto Lais Izabel. "Alu-polymerase chain reaction genomic fingerprinting in neuroblastoma." Thesis, University of Newcastle Upon Tyne, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.366679.

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9

Erill, Sagalés Ivan. "High-speed Polymerase chain reaction in CMOS-compatible chips." Doctoral thesis, Universitat Autònoma de Barcelona, 2002. http://hdl.handle.net/10803/3031.

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En la última década del siglo XX, el campo de los microsistemas para análisis total (µ-TAS) y, más concretamente, el de los DNA-chips ha adquirido una importancia preponderante en el ámbito de los microsistemas. En gran parte, el creciente interés por estos dispositivos se debe a las substanciales mejoras que prometen: análisis más rápidos, baratos y automatizados, pero también es debido a la posibilidad de implementar técnicas analíticas antes impensables (e.g. chips de hibridación). En el caso particular de los DNA-chips, se han desarrollado prototipos funcionales para PCR, LCR, electrofores

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10

Aydin, Gamze. "Detection Of Genetically Modified Maize Via Polymerase Chain Reaction." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12605495/index.pdf.

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In recent years, foods produced by genetic engineering technology have been on the world food market. The biosafety aspects, regulations, and labelling of these foods are still contentious issues in most countries. It is necessary to have approval for the use of GMOs in the production of food. Thus, detection and quantification of GMOs play crucial role for developing regulations on GM foods. In this study, raw and processed maize samples were analysed for genetic modification using a DNA based detection method, the Polymerase Chain Reaction. Ten raw food and 18 processed maize food incl

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11

Ballagi-Pordány, András. "Application of polymerase chain reaction (PCR) in veterinary virology /." Uppsala : Sveriges lantbruksuniv, 1995. http://epsilon.slu.se/avh/1995/91-576-4997-9.gif.

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12

Woolford, Alison Jane. "Use of polymerase chain reaction for detection of mycobacteria." Thesis, University of Surrey, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308497.

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13

Gurram, Neil (Neil K. ). "A mathematical model of polymerase chain reaction induced stutter." Thesis, Massachusetts Institute of Technology, 2016. http://hdl.handle.net/1721.1/106012.

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Thesis: M. Eng., Massachusetts Institute of Technology, Department of Electrical Engineering and Computer Science, 2016.<br>This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.<br>Cataloged from student-submitted PDF version of thesis.<br>Includes bibliographical references (page 48).<br>This is a thesis on understanding stutter present in capillary electropherogram readouts as this methodology forms the basis of current DNA fingerprinting. The readouts come from taking samples of various initial temp

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14

Lewis, Monte. "Thermal cycling design alternatives for the polymerase chain reaction." College Park, Md. : University of Maryland, 2005. http://hdl.handle.net/1903/3061.

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Thesis (M.S.) -- University of Maryland, College Park, 2005.<br>Thesis research directed by: Dept. of Mechanical Engineering. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.

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15

Way, Jaw-Shiow Chu. "Specific detection of Salmonella by multiplex polymerase chain reaction." Diss., The University of Arizona, 1993. http://hdl.handle.net/10150/186207.

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This study evaluates the use of multiplex polymerase chain reaction (PCR) technology for detection of Salmonella species in pure cultures and also in environmental samples. Three sets of oligonucleotide primers were used in the PCR assay: PhoP primers specific to a 299 bp region in the phoP/phoQ loci of coliform pathogenic bacteria such as Salmonella, Shigella, E. coli and Citrobacter species, served as a presumptive indicator of enteric bacteria. Subsequently Hin and H-1i primers, which targeted a 236 bp region of the hin/H2 gene, and a 173 bp region of the H-1-i flagellar gene in Salmonella

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16

Sikorsky, Jan A. "Effect of DNA base modification on polymerase chain reaction efficiency and fidelity." Huntington, WV : [Marshall University Libraries], 2005. http://www.marshall.edu/etd/descript.asp?ref=554.

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17

Muwonge, Abubaker. "Detection Of Genetically Modified Potatoes By The Polymerase Chain Reaction." Master's thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/12605783/index.pdf.

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Quite a number of important crops have been genetically modified with genes for agronomically important traits, such as insect and viral resistance. As the numbers of genetically modified foods continue to increase on the market, the need for rapid development of GMO detection methods is indispensable. This study was carried out to detect if genetically modified potatoes exist on food market in Turkey. Thirty samples from different places were collected. Using a DNA based PCR method, potato samples were examined for the presence of 35S promoter, Nos terminator, neomycin phosphotransferase

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18

White, Adam. "Development and application of microfluidic single-cell polymerase chain reaction." Thesis, University of British Columbia, 2015. http://hdl.handle.net/2429/55734.

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Methods for single-cell analysis are critical to revealing cell-to-cell variability in biological systems, such as during development or onset of disease, where the characteristics of heterogeneity and minority cell populations are obscured by population-averaged measurements. Analysis of individual cells has been limited due to challenges associated with small amounts of starting material, combined with the cost and throughput required to examine large numbers of cells. Microfluidic approaches are well suited to single-cell analysis, providing increased sensitivity, economy of scale, and auto

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19

Tsang, Tsui-ying Stella, and 曾璀瑩. "Application of quantitative polymerase chain reaction in the diagnosisof thalassaemia." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B45010948.

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20

Gamma, Torres Rafael Enrique. "Application of polymerase chain reaction to rhinovirus detection and analysis." Thesis, University of Essex, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293589.

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21

Koc, Yasemin. "Optimization of continuous flow polymerase chain reaction with microfluidic reactors." Thesis, Imperial College London, 2008. http://hdl.handle.net/10044/1/8184.

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The polymerase chain reaction (PCR) is an enzyme catalyzed technique, used to amplify the number of copies of a specific region of DNA. This technique can be used to identify, with high-probability, disease-causing viruses and/or bacteria, the identity of a deceased person, or a criminal suspect. Even though PCR has had a tremendous impact in clinical diagnostics, medical sciences and forensics, the technique presents several drawbacks. For example, the costs associated with each reaction are high and the reaction is prone to contamination due to its inherent efficiency and high sensitivity. B

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22

Allmann, Michael. "Applications of the polymerase chain reaction (PCR) in food chemistry /." [S.l.] : [s.n.], 1994. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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23

Mello, Richard. "IDENTIFICATION OF DQ ALPHA POLYMORPHISM USING THE POLYMERASE CHAIN REACTION." VCU Scholars Compass, 1991. https://scholarscompass.vcu.edu/etd/5213.

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This was a study of detection systems for DQ alpha HLA polymorphism that could be exploited for the demonstration of simulated chimerism. Polymorphic segments of DO alpha DNA were amplified by the polymerase chain reaction (PCR). Simulated chimerism was represented by a mixture of minor and major component DNA. The goal was to detect minor component DNA in the presence of major component DNA utilizing various laboratory techniques. Techniques studied included probe strip typing with the AmpliType HLA-DO Alpha test kit, allele-specific amplification, polyacrylamide gel electrophoresis, restrict

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24

Tsang, Tsui-ying Stella. "Application of quantitative polymerase chain reaction in the diagnosis of thalassaemia /." View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36433895.

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25

Tsui, Wai-yan. "Determination of PTEN mutations in prostate cancer in Chinese." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23736173.

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26

顔鴻儀 and Hung-yee Ngan. "Molecular diagnosis of penicilliosis marneffei." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970035.

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27

Basten, Graham Paul. "Induction of phase II enzymes by isothiocyanates : an investigation using quantitative RT-PCR." Thesis, University of East Anglia, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393316.

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28

Ngan, Hung-yee. "Molecular diagnosis of penicilliosis marneffei." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23595978.

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McMullen, Kevin Patrick. "Inhibitory effects of food matrices on real-time reverse transcription polymerase chain reaction detection of foodborne viruses." [Tampa, Fla. : s.n.], 2003. http://purl.fcla.edu/fcla/etd/SFE0000100.

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30

Sonmezalp, C. Zeynep. "Detection Of Genetically Modified Insect Resistant Tomato Via Polymerase Chain Reaction." Master's thesis, METU, 2004. http://etd.lib.metu.edu.tr/upload/3/12605493/index.pdf.

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Tomato, which is one of the most important component of human diet, has been genetically modified to develop some properties like delayed ripening and insect resistance. In order to give a choice to the consumer, it is necessary to detect and label GM foods. This study was carried out to detect genetically modified tomato samples purchased from different food markets of Turkey. PCR method was used to detect genetically modified insect resistant tomatoes. The DNAs of collected samples were isolated according to CTAB DNA extraction protocol and the amplification capacity of isolated sa

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31

Duman, Zeynep. "Polymerase Chain Reaction (pcr) For Detection Of Borrelia Burgdorferi Sensu Lato." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/2/12609199/index.pdf.

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The present study aimed detection of a human pathogen B. bugdorferi sensu lato species in suspected Lyme borreliosis (LB) patients in Turkey by PCR analysis and supportive serologic tests. The 152 clinical samples (140 serum and blood, 10 cerebrospinal fluid (CSF), 1 synovial fluid, 1 skin biopsy specimens) from 140 patients sent from 22 different cities of Turkey to The Spirochetal Diseases Diagnosis Laboratory of Central Veterinary Control and Research Institute were analysed. Serum samples were subjected to ELISA with a commercial kit and all of the blood, CSF, synovial fluid and skin biops

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32

Ros, Bascuñana Carlos. "Diagnostic application of the polymerase chain reaction (PCR) in veterinary microbiology /." Uppsala : Swedish Univ. of Agricultural Sciences (Sveriges lantbruksuniv.), 1997. http://epsilon.slu.se/avh/1997/91-576-5247-3.gif.

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Walker, Ken R. "Rapid detection of Listeria monocytogenes in salad by polymerase chain reaction." Auburn, Ala, 2005. http://repo.lib.auburn.edu/2005%20Summer/master's/WALKER_KEN_28.pdf.

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34

Phaneuf, Christopher. "Infrared laser-mediated polymerase chain reaction in a polymer microfluidic device." Diss., Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/53068.

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The ability to rapidly, sensitively, and accurately detect the presence of a pathogen is a vital capability for first responders in the assessment and treatment of scenarios such as disease outbreak and bioterrorism. Nucleic acid tests such as the polymerase chain reaction (PCR) are supplanting traditional techniques due to the improved speed, specificity, sensitivity, and simplicity. Still, amplification by PCR is often the bottleneck when processing genetic samples. Conventional PCR machines are bulky, slow, and consume large reagent volumes and an affordable, compact, efficient, easy-to-use

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35

Tully, Gillian. "DNA profiling for forensic identification : evaluation of polymerase chain reaction methods." Thesis, Cardiff University, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.264882.

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36

Diss, Timothy Charles. "The polymerase chain reaction in the characterisation and diagnosis of lymphomas." Thesis, University College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.338653.

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Organji, Sameer R. A. "Detection of #Beta#-lactam resistant Streptococcus pneumoniae by polymerase chain reaction." Thesis, University of Bristol, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.297824.

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Sim, Steven Poh Chuen. "An integrated chip-based device for droplet-flow polymerase chain reaction." Thesis, Imperial College London, 2016. http://hdl.handle.net/10044/1/56111.

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The polymerase chain reaction (PCR) is an important in-vitro technique in molecular biology for amplifying trace quantities of deoxyribonucleic acid (DNA). PCR is carried out by mixing the DNA molecules to be amplified with primers, polymerase enzymes and deoxynucleotide triphosphates (dNTPs) in a suitable buffer solution. A conventional thermal-cycler is then used to cycle the PCR mixture between multiple temperatures for denaturation, annealing and extension. Bench-top thermal cyclers have large thermal masses and use large sample volumes, leading to overly long cycling times, excessive ener

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39

Liao, Yu-Hua. "Polymerase chain reaction based cloning of acetate kinase in propionibacterium acidipropionici." The Ohio State University, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=osu1072778140.

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Holladay, Ervin Blair. "Viral gene detection in oral neoplasms using the polymerase chain reaction." The Ohio State University, 1991. http://rave.ohiolink.edu/etdc/view?acc_num=osu1345129906.

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Holladay, E. Blair. "Viral gene detection in oral neoplasms using the polymerase chain reaction /." The Ohio State University, 1991. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487694702781994.

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42

Liao, Yu-Hua. "Polymerase chain reaction based cloning of acetate kinase in Propionibacterium acidipropionici /." Connect to this title online, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1072778140.

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43

Liu, Tingting. "Electrokinetic Real-Time Polymerase Chain Reaction Toward Point-Of-Care Diagnosis." Diss., The University of Arizona, 2015. http://hdl.handle.net/10150/579083.

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Rapid diagnosis of infectious disease and timely initiation of proper clinical antibiotic treatment is the determinant in obtaining the optimal clinical outcomes and reducing emergences of multidrug-resistant organisms. In particular, acute infections require the detection to be accomplished in limited time with high sensitivity due to the low concentration of organisms causing the infections. Real-time Polymerase Chain Reaction can provide quantitative identification of specific genetic materials and has revolutionized clinical microbiology laboratory diagnosis. It is becoming a standard for

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44

Su, Ying-Tu, and 蘇英圖. "Application of MicroEmusification：Emulsion Polymerase Chain Reaction." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/51940901593932821213.

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碩士<br>國立臺灣海洋大學<br>機械與機電工程學系<br>97<br>In this thesis, soft lithography technique was used to produce a micro emulsion chip. This chip can handle tiny volume of DNA solution for polymerase chain reation (ePCR). The DNA solution is dispersed in the emulsified droplets in a continuous oil phase, and the droplets become reaction space for PCR. As a result, the consistency of the multiple types of DNA templates existing in the original reaction space can be improved by emulsification process. Also, it will reduce competitive sequence interference and improve DNA magnification. Differing from usual m

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45

Gi, Kao Li, and 高麗姬. "ABO Genotyping for Mutiplex Polymerase Chain Reaction." Thesis, 1999. http://ndltd.ncl.edu.tw/handle/82466547226309854097.

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碩士<br>中央警察大學<br>刑事警察研究所<br>87<br>Abstract ABO blood group is clinically the most important blood group system in transfusion medicine including fours common different phenotypes . It has been a valuable tool in transfusion medicine , physical anthropology , disputed parentage testing , human identification , and in forensic analysis . Historically , forensic and clinical labatories utilize serological techniques to identify ABO blood type . The testing of forensic samples for ABO types using serological techniques has several drawbacks . We has simplified the identification of ABO t

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46

Chen, Jhao-Rong, and 陳昭榮. "Micro Rayleigh-Bénard polymerase chain reaction system." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/05888212591754965876.

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碩士<br>國立清華大學<br>工程與系統科學系<br>93<br>Polymerase chain reaction (PCR) is a molecular biological method for the in vitro amplification of nucleic acid molecule. In this thesis, the research object was to design a micro PCR system which involved a Rayleigh-Bénard convection PCR chip, measurement circuits, and temperature control circuits. Rayleigh-Bénard convection PCR chip was easy to be fabricated, and the sample solution in it can transit its temperature immediately. Thus, the faster the speed of flow is, the higher heating and cooling rate is. Rayleigh-Bénard convection PCR chip can be divid

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Tao, Yo-Shen, and 刁宥升. "Design Of Polymerase Chain Reaction System Controller." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/76827215729605694932.

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碩士<br>國立暨南國際大學<br>電機工程學系<br>90<br>DNA analysis is a crucial process for biotechnology. Polymerase chain reaction (PCR) is an imperative step for performing DNA analysis. PCR can proliferate a small quantity of DNA sample to a large quantity enough for DNA analysis in a short time. Our goal is to develop a PCR system chip by integrating standard CMOS process and MEMS process. In this thesis, an 8-bit 4-level pipelined programmable micro-controller for temperature control during PCR process is designed by HDL description and implemented by using field programmable gate array (FPGA). T

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Gao, Li-Ji, and 高麗姬. "ABO Genotyping for Multiplex Polymerase Chain Reaction." Thesis, 1998. http://ndltd.ncl.edu.tw/handle/7433nn.

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碩士<br>中央警察大學<br>刑事警察研究所<br>87<br>ABO blood group is clinically the most important blood group system in transfusion medicine including fours common different phenotypes . It has been a valuable tool in transfusion medicine , physical anthropology , disputed parentage testing , human identification , and in forensic analysis . Historically , forensic and clinical labatories utilize serological techniques to identify ABO blood type . The testing of forensic samples for ABO types using serological techniques has several drawbacks . We has simplified the identification of ABO types by taking advan

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49

Lin, Min-Han. "PDMS (polydimethylsiloxane) Based Polymerase Chain Reaction Component Design." 2005. http://www.cetd.com.tw/ec/thesisdetail.aspx?etdun=U0001-2707200515463700.

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張澔任. "Detection of Chlamydophila abortus by polymerase chain reaction." Thesis, 2000. http://ndltd.ncl.edu.tw/handle/59334353234665564833.

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碩士<br>國立中興大學<br>獸醫微生物學研究所<br>88<br>The purpose of this study was to develop a specific polymerase chain reaction for rapid detection of Chlamydophila abortus. We selected the published chlamydial omp A and 16S-23S rRNA gene sequences from GeneBank, and respectively aligned these sequences to find suitable regions for Chlamydophila abortus-specific primer design. The alignment of 16S-23S rRNA gene sequences revealed no suitable region for primer design, while the alignment of omp A gene　sequences revealed many suitable regions for Chlamydophila abortus- specific primer design. We designed sever

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