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Autor: Grafiati

Data publikacji: 7 grudnia 2024

Data aktualizacji: 31 lipca 2025

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1

Helin, S., E. Arponen, J. Rajander, J. Aromaa, S. Johansson, and O. Solin. "Increased target volume and hydrogen content in [11C]CH4 production." Helmholtz-Zentrum Dresden - Rossendorf, 2015. http://nbn-resolving.de/urn:nbn:de:bsz:d120-qucosa-166344.

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Introduction High starting radioactivity is usually advantageous for producing radiopharmaceuticals with high specific radioactivity. However, the [11C]CH4 yields from N2-H2 gas target fall short from theoretical amounts, as calculated from the cross section for the well-known 14N(p,α)11C nuclear reaction1. The beneficial effect of increased target chamber temperature on [11C]CH4 yields has recently been brought forward by us2 and others3. In addition to the temperature effect, our attention has also been on the hydrogen content factor. This study intends to examine the N2-H2 target performan

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Rêgo, de Vasconcelos Bruna. "Phosphates-based catalysts for synthetic gas (syngas) production using CO2 and CH4." Thesis, Ecole nationale des Mines d'Albi-Carmaux, 2016. http://www.theses.fr/2016EMAC0004/document.

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Parmi les produits issus de la biomasse ou de la transformation des déchets organiques, le CO2 et le CH4 sont des intermédiaires chimiques importants qui ont de forts impacts environnementaux. En effet, ils sont les principaux gaz responsables de l'effet de serre et leur atténuation est un enjeu majeur. Une voie intéressante pour la valorisation de ces gaz est le reformage à sec du méthane (DRM), qui convertit le CO2 et le CH4 en gaz de synthèse (mélange d'hydrogène et de monoxyde de carbone). Ce mélange peut être utilisé pour plusieurs applications telles que la production de méthanol, d'éthe

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GOMEZ, CAMACHO CARLOS ENRIQUE. "Enhancement of bioenergy production (H2 + CH4) from organic waste in anaerobic fermentation processes." Doctoral thesis, Politecnico di Torino, 2019. http://hdl.handle.net/11583/2738393.

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YAMASHITA, Hiroshi. "Numerical Study on NOx Production of Transitional Fuel Jet Diffusion Flame." The Japan Society of Mechanical Engineers, 2000. http://hdl.handle.net/2237/8999.

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Gregoire, Manon. "Valorisation catalytique du CO2 via l’hydrogénation pour la production de méthane." Electronic Thesis or Diss., Littoral, 2024. http://www.theses.fr/2024DUNK0713.

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Cette étude porte sur la valorisation du CO2 par le procédé de méthanation. Elle vise à développer des matériaux catalytiques efficaces et stables pour cette réaction. L’espèce active utilisée dans différentes familles de matériaux sera le nickel. Dans un premier temps, nous avons focalisé notre travail sur des catalyseurs au nickel supporté sur différentes silices afin d’étudier l’influence de la taille des particules. Le premier, Ni/SiO2 est le catalyseur classiquement utilisé de Ni sur de la silice commerciale avec des tailles de particules métalliques d’environ 12 nm assez élevées et situé

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Chhajed, Pawan. "Diffusion Characterization of Coal for Enhanced Coalbed Methane Production." OpenSIUC, 2011. https://opensiuc.lib.siu.edu/theses/645.

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This thesis explores the concept of displacement of sorbed methane and enhancement of methane recovery by injection of CO2 into coal, while sequestering CO2. The objective of this study was to investigate the diffusion behavior of San Juan Basin coal under single and competitive gas environments. The movement of gas in a coalbed reservoir starts in the coal matrix with diffusion towards the naturally occurring cleat network surrounding the matrix blocks. The gas production potential from coalbed reservoirs under different gas environments was, therefore, estimated by studying the diffusion beh

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Hao, Yushan. "Characterization of Peat Bog CO2 and CH4 Production Potentials in relation to Peat Physico-chemical Properties and Vegetation Composition." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1562338709421684.

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Filho, Adibe Luiz Abdalla. "Produção de gases, síntese microbiana pelo radiofósforo e digestibilidade do babaçu e mofumbo em dietas de ovinos." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/64/64133/tde-05052015-094343/.

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Quando a escassez de alimentos em pastagens naturais compromete a alimentação animal, os pequenos ruminantes podem incorporar em suas dietas as folhas de outras plantas, como árvores e arbustos, muitas delas ricas em metabólitos secundários como taninos e que ainda carecem de estudos acerca de seus efeitos na produtividade animal. Com o objetivo de verificar a possibilidade de utilização das folhas de Orbignya phalerata (Babaçu) e Combretum leprosum (Mofumbo) na alimentação e avaliar o efeito da inclusão na produção de ovinos, dois estudos foram conduzidos no Laboratório de Nutrição Animal do

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Wei, Tzu-Hsiang Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Transient production of biopharmaceutical proteins." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2009. http://handle.unsw.edu.au/1959.4/43708.

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The creation of stable mammalian cell lines for biopharmaceutical production often require several months, and is unfavourable for the rapid production of multiple drug candidates for screening in the early stages of development. Biopharmaceutical production by transient transfection provides a possible alternative of quickly producing these early stage drug candidates. The Epi-CHO transient expression system, which consists of a Chinese hamster ovary (CHO) cell line (CHO-T) expressing the murine polyomavirus Large T-Antigen (LT), emonstrated enhanced transient recombinant protein production.

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Kunaparaju, Raj Kumar Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Epi-CHO, an episomal expression system for recombinant protein production in CHO cells." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2008. http://handle.unsw.edu.au/1959.4/41499.

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The current project is to develop a transient expression system for Chinese Hamster Ovary (CHO) cells based on autonomous replication and retention of plasmid DNA. The expression system, named Epi-CHO comprises (1) a recombinant CHO-K1 cell line encoding the Polyoma (Py) virus large T-Antigen (PyLT-Ag), and (2) a DNA expression vector, pPy/EBV encoding the Py Origin (PyOri) for autonomous replication and encoding the Epstein-Barr virus (EBV), Nuclear Antigen-1 (EBNA-1) and EBV Origin of replication (OriP) for plasmid retention. The CHO-K1 cell line expressing PyLT-Ag, named CHO-T was adapted t

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Lima, e. Castro Paula Maria. "Optimisation of CHO cell growth and recombinant interferon-γ production". Thesis, University College London (University of London), 1993. http://discovery.ucl.ac.uk/1317969/.

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The optimisation of recombinant protein production by animal cell cultures is important for the economic feasibility of these processes. Simultaneously with product yield, product authenticity is a crucial aspect to consider as it may per se affect the therapeutic value of such proteins. More defined culture media are being developed, particularly to ensure batch product consistency. A Chinese Hamster Ovary cell line (CHO 320) producing human interferon-γ (IFN-γ), a glycosylated protein, was chosen to investigate the effects of the culture environment on (I) cell growth, (2) product yield and

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12

Akin, Ilina V. "Measurement Of The Cross Section Ratio Chi-c2/chi-c1 For Prompt Chi-c Production With Cms Experiment." Phd thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614979/index.pdf.

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The prompt production of &chi<br>c quarkonia is studied in proton-proton collisions at 7 TeV, using data collected by CMS in 2011 corresponding to an integrated luminosity of 4.6 fb &minus<br>1. The &chi<br>c mesons are reconstructed through their radiative decays to J/&psi<br>and photon with J/&psi<br>&rarr<br>&mu<br>+&mu<br>&minus<br>. The photons are reconstructed through their conversion in electron-positron pairs in the tracking detector which gives a mass resolution sufficient for resolving these states. The ratio of the prompt production cross sections for the &chi<br>c1 and &chi<br>c2

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Lemoigne, Yves. "Hadro-production d'états CHI du charmonium auprès du SPS." Paris 11, 1987. http://www.theses.fr/1987PA112333.

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L’observation d’états CHI du charmonium en hadro-production, nécessite une résolution très fine pour séparer des états de masses très proches (45 MeV entre deux états CHI de masse supérieure à 3510 MeV). L’expérience WA11 a pu séparer les états CHI(3510) et CHI(3555), mesurer leur section efficace en production π – bérylium à 185 GeV / c et chiffrer la contribution de ces états à la production du J /ψ (17,7 % et 12,8 % ). L’étude détaillée qui a été menée sur les événements CHI(3555) n’est pratiquement produit que par fusion gluon – gluon, alors que le CHI(3510) est issu également des fusions

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14

Connolly, Helen. "The formation of halocarbons by marine organisms." Thesis, Bangor University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280676.

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Szula, Ewa. "Metabolic profiling and imaging of CHO cells for fusion protein production." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/metabolic-profiling-and-imaging-of-cho-cells-for-fusion-protein-production(ec83142c-0d97-437e-8d0f-d767887bcde5).html.

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Fc-fusion proteins (e.g. EPO-Fc) are the most often created fusion proteins due to their beneficial biological and pharmacological properties. The economic success of Fc-fusion proteins and other biopharmaceuticals production however, greatly depends on a robust, low-cost and highly effective protein mammalian cell extraction system . Understanding of how cells respond to a protein production environment based on metabolic profiles provides new goals for bioengineering of cell lines for best performance in biomanufacturing. Furthermore, insights on how individual cell metabolism and therefore

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Yang, Ming. "Ammonia effects on CHO cell growth, metabolism, erythropoietin production and glycosylation." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ56157.pdf.

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Hanson, Eleanor. "The epigenetics (histone PTMs) of therapeutic protein production in CHO cells." Thesis, University of Sheffield, 2019. http://etheses.whiterose.ac.uk/22945/.

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Chinese hamster ovary (CHO) cells are an important host cell system for the manufacturing of biopharmaceuticals. Epigenetics have been shown to have an important role in controlling gene expression in CHO cells, and thus affect the rate of protein production. It is hoped that by understanding the role of epigenetics in CHO cells improvements can be made in the production of biopharmaceuticals. In this work we have studied how histone PTMs differ between cell lines and throughout culture. These changes have then been correlated with growth rate, cell size and specific productivity. Mass spectro

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Maria, Sophie. "Développement d'un bioprocédé continu couplant la production et la purification d'un anticorps recombinant." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0898/document.

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Les anticorps monoclonaux sont une classe de bio médicaments en plein essor. Leur production est largement étudiée afin d’obtenir des rendements de plus en plus élevés et de réduire les coûts. Cette thèse décrit le développement d’un procédé complet en continu, de la production d’anticorps recombinants par des cellules mammifères jusqu’à leur purification. L’objectif est de coupler la culture cellulaire en mode perfusion à la purification par chromatographie semi-continue. Le développement du procédé se fait en bioréacteur avec une lignée de cellules d’ovaires de hamster chinois (CHO-DP12) tra

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Porncharoennop, Chompoonuth. "Metabolite profiling associated with productive recombinant CHO cell culture." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/metabolite-profiling-associated-with-productive-recombinant-cho-cell-culture(3bb6bdaf-d8dc-4249-b77b-159e9e77307a).html.

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A positive correlation between the flux of TCA cycle and productivity of Chinese Hamster Ovary (CHO) cells has been reported. Earlier work in this laboratory revealed that supplementation with nutrients that enter the TCA cycle (combination of glucose (Glc), pyruvate (Py), aspartate (Asp), asparagine (Asn) and glutamate (Glu)) significantly increased maximum viable cell density and antibody production of recombinant CHO cells. Increased amounts of extracellular citrate was associated with feeding conditions. It was hypothesized that increased flux through the TCA cycle and related metabolism w

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Ottosson, Anton. "Integration of Hydrogen Production via Water Electrolysis at a CHP Plant : A feasibility study." Thesis, Luleå tekniska universitet, Institutionen för teknikvetenskap och matematik, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:ltu:diva-83717.

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Hydrogen gas (H2), that is not produced from fossil oil or natural gas, is expected to become a cornerstone in the energy transition strategy in Europe. The recent years, technological and economic advances in the electrolyzer area, along with political and corporate support, have put H2 at the forefront of many countries’ climate change agenda. Consequently, green H2 is poised to play a large role in the coming energy transition to combat climate change. The possible advantages of integrating H2 production with a combined heat and power plant, or CHP, is investigated in this study. More preci

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Melchior, Frédéric. "Obtention et production d'anticorps monoclonaux pour la détéction directe d'alcaligenes eutrophus CH34." Metz, 1998. http://docnum.univ-lorraine.fr/public/UPV-M/Theses/1998/Melchior.Frederic.SMZ9844.pdf.

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Alcaligenes eutrophus CH34, bactérie à gram négatif et ayant un métabolisme chimiolithotrophe facultatif, possède deux mégaplasmides pMOL28 et pMOL30 de résistances aux métaux lourds (Cd, Zn, Co, Ni, Cu, Tl, chromates, Hg et Pb. Des membranes externes de cette bactérie, cultivée avec (0,0096 g/l) ou sans fer, ont été isolées par la méthode de Forsberg et al. (1970) légèrement modifiée par Falla et al. (1990). Des marqueurs spécifiques, KDO (3-céto-2-déoxyoctonate) et SDH (succinate déshydrogénase), de la membrane externe et de la membrane cytoplasmique respectivement ont été dosés. Ils indique

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MELCHIOR, FREDERIC Bauda Pascale. "OBTENTION ET PRODUCTION D'ANTICORPS MONOCLONAUX POUR LA DETECTION DIRECTE D'ALCALIGENES EUTROPHUS CH34 /." [S.l.] : [s.n.], 1998. ftp://ftp.scd.univ-metz.fr/pub/Theses/1998/Melchior.Frederic.SMZ9844.pdf.

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Michel, Johannes. "The Sustainability of Decentralized Bioenergy Production : Case Study: The 'Bioenergy Village' Bollewick." Thesis, Uppsala universitet, Institutionen för geovetenskaper, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-194437.

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The concept of Sustainable Development is an interdisciplinary science. Transcending various academic fields the concept shows paths how the needs of present and future generations can be met through economic development on a finite natural resource base. Global warming and rising sea levels are just two of a series of phenomena that are directly attributable to human-induced increasing greenhouse gas levels in the atmosphere as consequence of the combustion of fossil fuels. Therefore, reducing greenhouse gas emissions through the use of renewable resources such as bioenergy are of vital impor

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Sallent, Roger. "Return temperature influence of a district heating network on the CHP plant production costs." Thesis, University of Gävle, University of Gävle, Department of Technology and Built Environment, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-4989.

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<p><strong></strong>The aim of this Project is to study the influence of high return temperatures in district heating on the costs for heat and power production in a CHP plant.When the temperatures of the water coming back to the heating plant are so high, the overall performance of heat and power production is decreased and, consequently, also the production costs. Along the project, the influence of this temperature on the different parts of a CHP plant are analysed as well as the economical impact it has. At the same time, some general impacts on the entire network are mentioned.</p><p> </p

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Jonckeau, Agathe. "Production, purification et caractérisation d’une gonadotropine chorionique équine recombinante à usage vétérinaire." Thesis, Bordeaux, 2016. http://www.theses.fr/2016BORD0208.

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Des hormones gonadotropiques sont utilisées pour la maîtrise de la reproduction dans le domaine vétérinaire. Ces hormones sont actuellement extraites de tissus ou de fluides animaux. L’entreprise CEVA Santé Animale a récemment fait le choix stratégique de produire ces hormones par voie recombinante. L’objectif de cette étude était d’obtenir une gonadotropine chorionique équine recombinante, reCG, pure et biologiquement active, à partir d’une lignée de cellules mammifères CHO. Les étapes de production, de purification et de caractérisation de l’hormone recombinante ont été développées. Les cell

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Lamotte, Damien. "Production et glycosylation de l'interféron-gamma humain par des cellules CHO cultivées en bioréacteurs discontinus et perfusés : influence des conditions opératoires et du potentiel de glycosylation des cellules." Vandoeuvre-les-Nancy, INPL, 1997. http://www.theses.fr/1997INPL061N.

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L’objectif général de ce travail est l'étude de la production et de la glycosylation de l'interféron-gamma humain recombinant (IFN) exprimé par différentes lignées de cellules animales CHO (Chinese Hamster Ovary) cultivées en bioréacteurs discontinus et perfusés. La première partie traite de l'influence de l'intégration du gène de l'[alpha]2,6-sialyltransferase ([alpha]2,6-ST) dans une lignée CHO produisant l'IFN sur les cinétiques de cultures en réacteurs discontinus (croissance cellulaire, consommation des nutriments et production d'IFN), et sur l'état de glycosylation de la glycoprotéine pr

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Binge, Alexandra. "Development of endogenous and synthetic CHO promoter expression systems for recombinant protein production." Thesis, University of Kent, 2018. https://kar.kent.ac.uk/66640/.

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Chinese hamster ovary (CHO) cells are the most commonly used mammalian cell expression system for the industrial production of biotherapeutic recombinant proteins. Mammalian expression systems are a popular choice for the production of complex biopharmaceuticals due to their ability to correctly fold and assemble recombinant proteins and to carry out necessary human-like post-translational modifications. Traditionally, viral promoters are used to drive transgene expression in mammalian cells. This study reports on the investigation of endogenous CHO promoters as potential alternatives to the c

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De, Villiers Ann-Marie. "Production and glycosylation of a recombinant protein from Chinese hamster ovary (CHO) cells." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71663.

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Thesis (MScEng)--Stellenbosch University, 2012.<br>ENGLISH ABSTRACT: Recombinant glycoproteins are important biopharmaceuticals, providing solutions for numerous previously untreatable illnesses, in everything from cancer to infertility. Most recombinant biopharmaceuticals are produced in mammalian cells due to their ability to provide the correct post-translational processing for use in humans. The post-translation processing influences many of the protein’s properties including pharmacokinetics, bioactivity, secretion, half-life, solubility, recognition and antigenicity. The aim of this thes

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Stevens, Kristoffer. "Integratedenergy storage system for optimal energy production : A case study on Johannes CHP biofuel plant." Thesis, Högskolan i Gävle, Avdelningen för bygg- energi- och miljöteknik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:hig:diva-14906.

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This project served to analyze the effects that energy storage can have on energy production. The study was aimed at Johannes CHP bio fuel. Johannes produces electricity for the SE3 region and heat for the district heating in Gävle. The electricity market is the main driving factor for energy production. It is ideal for Johannes to produce as much electricity as possible during high Elspot prices. Two accumulator tanks are already installed at Johannes so the surplus of heat can be stored from high electrical production. This study served to utilize the planning horizons for the future Elspot

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Bjørnstad, Pål Marius. "Central exclusive production of χ mesons at LHCb". Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/central-exclusive-production-of-chi-mesons-at-lhcb(64c3f7d8-3c71-4e4b-a592-69a6e27424d7).html.

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The LHCb experiment is a forward spectrometer at the Large Hadron Collider, covering a range of pseudorapidity 1:9 < η < 4:9. It has a very precise vertex detector called the VELO, near the interaction point. The monitoring software for the VELO is discussed in this thesis. In proton-proton interactions, Central Exclusive Production (CEP) is a process where the protons remain intact after the interaction, and an additional simple central system is produced. CEP processes are selected experimentally by requiring that there is no activity in the detector apart from the central system. The instal

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Goulart, Dickson C. "J/PSI production via chi sub C decays in fixed target proton-nucleus collisions." Cincinnati, Ohio University of Cincinnati, 2004. http://www.ohiolink.edu/etd/view.cgi?acc%5Fnum=ucin1100189506.

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Huang, Edwin P. C. Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Recombinant protein production utilising a metallothionein expression system and a Super-CHO cell line." Awarded by:University of New South Wales. School of Biotechnology and Biomolecular Sciences, 2006. http://handle.unsw.edu.au/1959.4/24940.

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A novel metal-inducible and amplifiable metallothionein (MT) expression system, pNK, was firstly optimised and characterised for the production of a reporter protein, human growth hormone (hGH) in a suspension CHO cell line grown in a serum-free media. The pNK-based hGH production was demonstrated in cadmium-free condition under various fermentation modes (batch, fed-batch and perfusion) and scales (flask to bench-top bioreactor). Improvement of specific productivity of recombinant protein from pNK was shown to be possible by addition of butyrate or substrate substitution of glutamine by gluta

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Almanan, Maha. "CD4+ T cell Production of IL-10 and Regulation of Immune Responses in Aging." University of Cincinnati / OhioLINK, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1535466924008943.

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Achaica, Santos Angelica, Huamanchumo Jackeline Liz Arce, Salcedo Angel De Jesús Calle, Huamán Rocio del Pilar Silva, and Xiuwen Zhou. "Hao Hao Chi." Bachelor's thesis, Universidad Peruana de Ciencias Aplicadas (UPC), 2020. http://hdl.handle.net/10757/654898.

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El trabajo realizado presenta un modelo de negocio que pertenece a la industria de alimentos y bebidas, y se dedicará a la producción y comercialización de bocaditos chinos congelados y empaquetados al vacío en el departamento de Lima, Perú. Mediante la recopilación de información obtenida del análisis PESTEL, se determinó la situación actual de los factores Macroentorno del país para estar preparados ante situaciones inusuales. Asimismo, se consideró relevante el estudio de las cinco fuerzas de Porter para abordar los factores de Microentorno que tiene relación con la industria que deseamos i

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Vidlund, Anna. "Sustainable production of bio-energy products in the sawmill industry." Licentiate thesis, KTH, Chemical Engineering and Technology, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-1734.

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<p>One of the great challenges facing society is to convert theglobal energy system to a sustainable process. Currently, 80%of the world´s energy is supplied through the combustionof fossil fuels. Not only are the fossil resources limited, theutilisation also increases the level of greenhouse gases in theatmosphere. The convertion to a sustainable energy system isproblematic since the technology needed to exploit mostnon-fossil energy sources is not yet fully developed, e.g.solar energy. Biofuel is an available renewable energy sourcewhich is already widely used in many countries. If an effect

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Lipscomb, Matthew L. "Perfusion cultures of recombinant CHO cells: Effects on specific productivity, production stability, and protein glycosylation." Diss., Connect to online resource, 2005. http://wwwlib.umi.com/dissertations/fullcit/3165829.

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Fox, Stephen Richard. "Active hypothermic growth : a novel means for increasing total recombinant protein production by CHO cells." Thesis, Massachusetts Institute of Technology, 2005. http://hdl.handle.net/1721.1/32333.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 2005.<br>Includes bibliographical references (p. 225-240).<br>Recombinant human glycoproteins produced by Chinese Hamster Ovary (CHO) cells are an important class of therapeutic molecules and investigating means of improving the production rate and product quality of these glycoproteins is therefore of great interest. Culturing CHO cells under mild hypothermia (30-33 ⁰C) leads to growth arrest in the G₀/G₁ phase of the cell cycle and, in some cases, causes an increase in specific productivity of recombinant

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ÖHMAN, AXEL. "Green hydrogen production at Igelsta CHP plant : A techno-economic assessment conducted at Söderenergi AB." Thesis, KTH, Skolan för industriell teknik och management (ITM), 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-299434.

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The energy transition taking place in various parts of the world will have many effects on the current energy systems as an increasing amount of intermittent power supply gets installed every year. In Sweden, just as many other countries, this will cause both challenges and opportunities for today´s energy producers. Challenges that may arise along with an increasingly fluctuating electricity production include both power deficits at certain times and regions but also hours of over-production which can cause electricity prices to drop significantly. Such challenges will have to be met by both

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Le, Floch François. "Production et glycosylation d'EPO par cellules CHO : caractérisation par électrophorèse capillaire au cours de procédés discontinus." Vandoeuvre-les-Nancy, INPL, 2003. http://docnum.univ-lorraine.fr/public/INPL_T_2003_LE_FLOCH_F.pdf.

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Ce travail a pour objectif d'améliorer la maîtrise de la glycosylation des protéines recombinantes produites par culture de cellules animales, ceci par l'étude de l'influence du procédé de culture sur la structure glycannique d'une protéine modèle: l'érythopoi͏̈étine (EPO) produite par cellules CHO. La construction du système d'étude a d'abord nécessité plusieurs étapes: la génération d'un clone CHO producteur d'EPO performant, la mise au point d'une méthode de purification de la protéine par immuno- affinité et l'adaptation d'une technique d'analyse de la glycosylation aux contraintes de notr

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Mathias, Sven [Verfasser]. "Identification, characterization and removal of intracellular secretion bottlenecks in industrial CHO production cell lines / Sven Mathias." Ulm : Universität Ulm, 2021. http://d-nb.info/1239737203/34.

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Abajyan, Anaida. "Characterization of altered cytokine production by memory CD4 T cells in NZBxW murine model of SLE." Doctoral thesis, Humboldt-Universität zu Berlin, 2019. http://dx.doi.org/10.18452/20094.

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Der systemische Lupus erythematodes (SLE) ist eine Autoimmunerkrankung, bei der eine Vielzahl an Organen betroffen sein kann. Hierbei spielen T-Zellen mit gestörter Zytokinproduktion, insbesondere von IL-2 und IFN-γ, eine besondere Rolle. Mit Fortschreiten der Krankheit sinkt die Anzahl an IL-2-Produzenten und gleichzeitig steigt die Anzahl an IFN-γ-Produzenten. Während die Rolle von IFN-γ in SLE bisher kontrovers diskutiert wird, wirkt sich die verringerte Produktion von IL-2 beispielsweise negativ auf regulatorische T-Zellen aus, was zur Pathogenese der Krankheit beiträgt. In dieser Arbeit

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Daianova, Lilia. "Lignocellulosic Ethanol Production Potential and Regional Transportation Fuel Demand." Licentiate thesis, Mälardalens högskola, Akademin för hållbar samhälls- och teknikutveckling, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-13176.

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Road traffic dominates in domestic Swedish transportation and is highly dependent on fossil fuels, petrol and diesel. Currently, the use of renewable fuels in transportation accounts for less than 6% of the total energy use in transport. The demand for bioethanol to fuel transportation is growing and cannot be met through current domestic production alone. Lignocellulosic ethanol derived from agricultural crop residues may be a feasible alternative source of ethanol for securing a consistent regional fuel supply in Swedish climatic conditions. This licentiate thesis focuses on regional transp

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Pereira, João Nuno dos Santos. "Establishing a high titer transient gene expression process in conditioned media for CHO-DG44 cells." Master's thesis, Faculdade de Ciências e Tecnologia, 2011. http://hdl.handle.net/10362/6149.

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Dissertação para obtenção do Grau de Mestre em Biotecnologia<br>Transient gene expression (TGE) allows for fast protein production in mammalian cells and has become a very important technology in the product development pipeline of biopharmaceuticals. Polyethylenimine (PEI) mediated, high-density transfections have allowed for transient processes exceeding ~300mg/L in CHO-DG44 cells. As such, the bottleneck of TGE is no more in the titers, but in the scale-up to volumes higher than 1L, because of the need for a medium exchange before transfection. It is known that if the transfection is done

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Elshereef, Abdalla Adel Sabry Abdelrazik Mohamed [Verfasser]. "Optimization of transient protein production by chemically transfected CHO suspension cells / Abdalla Adel Sabry Abdelrazik Mohamed Elshereef." Hannover : Technische Informationsbibliothek (TIB), 2018. http://d-nb.info/1166088472/34.

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Betts, Zeynep. "Assessment of the influence of chromatin elements on stability of recombinant protein production in amplified CHO cells." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/assessment-of-the-influence-of-chromatin-elements-on-stability-of-recombinant-protein-production-in-amplified-cho-cells(c7045083-e47b-4e64-8d96-71e59babd14d).html.

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Therapeutic proteins require proper folding and post-translational modifications (PTMs) to be effective and biologically active. Chinese hamster ovary (CHO) cells are the most frequently used host for commercial production of therapeutic proteins and DHFR-mediated gene amplification is extensively applied to generate cell lines with increased protein production. However, decreased protein productivity is observed unpredictably during the time required for scale-up with consequences for yield, time, finance and regulatory approval. Ubiquitous Chromatin Opening Elements (UCOEs) are DNA elements

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Elshereef, Abdalla [Verfasser]. "Optimization of transient protein production by chemically transfected CHO suspension cells / Abdalla Adel Sabry Abdelrazik Mohamed Elshereef." Hannover : Technische Informationsbibliothek (TIB), 2018. http://d-nb.info/1166088472/34.

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Agha, Mujtaba Hassan. "Integrated management of energy and production : scheduling of batch process and Combined Heat & Power (CHP) plant." Thesis, Toulouse, INPT, 2009. http://www.theses.fr/2009INPT050G/document.

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Dans un contexte de développement durable, la question énergétique constitue un des problèmes majeurs des décennies à venir. Bien que la solution pour faire face à la raréfaction de certaines ressources, l'augmentation globale de la demande l'augmentation des émissions de CO2, réside dans le développement des énergies renouvelables, il est clair que ces nouvelles technologies ne seront matures que dans plusieurs décennies. A court terme, les énergies fossiles demeureront la source principale d'énergie primaire. Il est donc essentiel de promouvoir de nouvelles méthodologies permettant une utili

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STRIPPOLI, LAURA. "Transposon-based technology enhances the generation of stable and high-producing CHO clones for industrial production of recombinant proteins and antibodies." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2015. http://hdl.handle.net/10281/68384.

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Proteine ricombinanti e anticorpi sono reagenti chiave per lo sviluppo di saggi immunodiagnostici. Tuttavia proteine che hanno modifiche post-transcrizionali complesse o più subunità, come gli anticorpi, sono raramente espresse in microrganismi. Infatti, per ottenere una corretta conformazione sono necessarie le modifiche post-transcrizionali tipiche delle cellule di mammifero. Negli ultimi anni sono stati sviluppati diversi metodi per il trasferimento genico, ma nonostante ciò è ancora difficile ottenere linee cellulari stabili e altamente produttive. Le strategie convenzionali si basano sull

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Saunders, Fay Louise. "An investigation into the role of chromatin modifying elements on the production of recombinant antibodies from CHO Cells." Thesis, Open University, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.533111.

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Bydén, William, and David Fridlund. "Carbon Negative Heat and Power with Biochar Production : An Economic Analysis of a Combined Pyrolysis and CHP plant." Thesis, KTH, Skolan för industriell teknik och management (ITM), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-279608.

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On the fourth of November 2016, The Paris Agreement entered into force, stating that nations worldwide should pursue efforts to limit the global temperature increase to 1,5 °C. Since then, the Intergovernmental Panel on Climate Change has specified that carbon dioxide removal, such as biochar sequestration, is necessary to achieve this goal. Biochar is a solid and porous material, rich in carbon, produced when biomass undergoes a process called pyrolysis and can, if buried in soil, sequester carbon for hundreds or even thousands of years while at the same time acting as a soil amendment. When

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