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Artykuły w czasopismach na temat „Protein and mRNA levels”

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Data publikacji: 6 września 2023

Data aktualizacji: 31 lipca 2025

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1

Gerstenfeld, L. C., M. H. Finer, and H. Boedtker. "Altered beta-actin gene expression in phorbol myristate acetate-treated chondrocytes and fibroblasts." Molecular and Cellular Biology 5, no. 6 (1985): 1425–33. http://dx.doi.org/10.1128/mcb.5.6.1425-1433.1985.

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Phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, was shown to have opposite effects on the cellular morphology and steady-state levels of beta-actin mRNA in embryonic chicken muscle fibroblasts and sternal chondrocytes. When fibroblasts were treated with PMA, they formed foci of densely packed cells, ceased to adhere to culture plates, and had significantly reduced levels of beta-actin mRNA and protein. Conversely, when treated with PMA, floating chondrocytes attached to culture dishes, spread out, and began to accumulate high levels of beta-actin mRNA and proteins. In the stern

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2

Gerstenfeld, L. C., M. H. Finer, and H. Boedtker. "Altered beta-actin gene expression in phorbol myristate acetate-treated chondrocytes and fibroblasts." Molecular and Cellular Biology 5, no. 6 (1985): 1425–33. http://dx.doi.org/10.1128/mcb.5.6.1425.

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Phorbol-12-myristate-13-acetate (PMA), a potent tumor promoter, was shown to have opposite effects on the cellular morphology and steady-state levels of beta-actin mRNA in embryonic chicken muscle fibroblasts and sternal chondrocytes. When fibroblasts were treated with PMA, they formed foci of densely packed cells, ceased to adhere to culture plates, and had significantly reduced levels of beta-actin mRNA and protein. Conversely, when treated with PMA, floating chondrocytes attached to culture dishes, spread out, and began to accumulate high levels of beta-actin mRNA and proteins. In the stern

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3

Willis, Dianna E., Erna A. van Niekerk, Yukio Sasaki, et al. "Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs." Journal of Cell Biology 178, no. 6 (2007): 965–80. http://dx.doi.org/10.1083/jcb.200703209.

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Subcellular regulation of protein synthesis requires the correct localization of messenger RNAs (mRNAs) within the cell. In this study, we investigate whether the axonal localization of neuronal mRNAs is regulated by extracellular stimuli. By profiling axonal levels of 50 mRNAs detected in regenerating adult sensory axons, we show that neurotrophins can increase and decrease levels of axonal mRNAs. Neurotrophins (nerve growth factor, brain-derived neurotrophic factor, and neurotrophin-3) regulate axonal mRNA levels and use distinct downstream signals to localize individual mRNAs. However, myel

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4

Maicas, E., F. G. Pluthero, and J. D. Friesen. "The accumulation of three yeast ribosomal proteins under conditions of excess mRNA is determined primarily by fast protein decay." Molecular and Cellular Biology 8, no. 1 (1988): 169–75. http://dx.doi.org/10.1128/mcb.8.1.169-175.1988.

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The suggestion that compensation for overabundant mRNA of the genes for Saccharomyces cerevisiae ribosomal protein (r-protein) L3, L29, or rp59 occurs by translation repression has been reinvestigated. First, analysis of the distribution of these three mRNAs in polysome profiles revealed no differences between normal and mRNA-overproducing strains, indicating that initiation of r-protein translation is not repressed under conditions of mRNA overaccumulation. Second, experiments involving radioactive pulse-labeling of proteins were done by using a modified method of data collection and analysis

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5

Maicas, E., F. G. Pluthero, and J. D. Friesen. "The accumulation of three yeast ribosomal proteins under conditions of excess mRNA is determined primarily by fast protein decay." Molecular and Cellular Biology 8, no. 1 (1988): 169–75. http://dx.doi.org/10.1128/mcb.8.1.169.

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The suggestion that compensation for overabundant mRNA of the genes for Saccharomyces cerevisiae ribosomal protein (r-protein) L3, L29, or rp59 occurs by translation repression has been reinvestigated. First, analysis of the distribution of these three mRNAs in polysome profiles revealed no differences between normal and mRNA-overproducing strains, indicating that initiation of r-protein translation is not repressed under conditions of mRNA overaccumulation. Second, experiments involving radioactive pulse-labeling of proteins were done by using a modified method of data collection and analysis

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6

Veletza, S. V., K. V. Nichols, I. Gross, H. Lu, D. W. Dynia, and J. Floros. "Surfactant protein C: hormonal control of SP-C mRNA levels in vitro." American Journal of Physiology-Lung Cellular and Molecular Physiology 262, no. 6 (1992): L684—L687. http://dx.doi.org/10.1152/ajplung.1992.262.6.l684.

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We have studied hormonal regulation of the surfactant protein C (SP-C) in fetal 18-dah rat lung explants. SP-C mRNA was detected in Northern blots with a specific rat SP-C cDNA probe and quantified by densitometry. Treatment of the explants with dexamethasone resulted in a dose-dependent increase of the SP-C mRNA level. Transcriptional assays have shown that the regulation of SP-C mRNA by dexamethasone involves a transcriptional step. Administration of the cAMP analogues, 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) or dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), produced

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7

Muller, M., J. Gauley, and John J. Heikkila. "Hydrogen peroxide induces heat shock protein and proto-oncogene mRNA accumulation in Xenopus laevis A6 kidney epithelial cells." Canadian Journal of Physiology and Pharmacology 82, no. 7 (2004): 523–29. http://dx.doi.org/10.1139/y04-059.

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In this study, we examined the effect of hydrogen peroxide on the accumulation of various mRNAs encoding heat shock proteins (hsps) and proto-oncogenes in Xenopus A6 kidney epithelial cells. Hydrogen peroxide treatment enhanced the accumulation of hsp90, hsp70, hsp30, c-jun, c-fos, and actin mRNAs with distinct temporal patterns. Although hsp70, c-fos, and c-jun mRNA levels peaked at 1–2 h before declining, hsp30 and hsp90 mRNA levels were maximal at 4–6 h. Other mRNAs, including heat shock cognate hsc70, immunoglobulin binding protein, and ribosomal L8, were unaffected. Treatment of kidney ce

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8

Laxminarayana, Dama, Islam U. Khan, Nilamadhab Mishra, Irene Olorenshaw, Kjetil Taskén та Gary M. Kammer. "Diminished Levels of Protein Kinase A RIα and RIβ Transcripts and Proteins in Systemic Lupus Erythematosus T Lymphocytes". Journal of Immunology 162, № 9 (1999): 5639–48. http://dx.doi.org/10.4049/jimmunol.162.9.5639.

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Abstract Deficient type I protein kinase A phosphotransferase activity occurs in the T cells of 80% of subjects with systemic lupus erythematosus (SLE). To investigate the mechanism of this deficient isozyme activity, we hypothesized that reduced amounts of type I regulatory (RI) isoform transcripts, RIα and RIβ, may be associated with a diminution of RIα and/or RIβ protein. Sixteen SLE subjects with a mean (±1 SD) SLE disease activity index of 12.4 ± 7.2 were studied. Controls included 16 normal subjects, six subjects with primary Sjögren’s syndrome (SS), and three subjects with SS/SLE overl

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9

Berger, Lloyd C., Jnanankur Bag, and Bruce H. Sells. "Translation of poly(A)-binding protein mRNA is regulated by growth conditions." Biochemistry and Cell Biology 70, no. 9 (1992): 770–78. http://dx.doi.org/10.1139/o92-117.

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Translational efficiency of a minor group of mRNAs is regulated by serum levels in 3T6 fibroblasts. Included within this group is the poly(A)-binding protein (PABP) mRNA. We analyzed the distribution of PABP mRNA in polysome profiles and found a large percentage of this mRNA to be translationally repressed in both actively growing (~ 60%) and resting cells (~ 70%). Elevated serum levels induced a distinct bimodal distribution of this mRNA between actively translated and repressed fractions. Similarly, treatment of cells with low doses of cycloheximide also generated a partial shift of represse

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10

Gygi, Steven P., Yvan Rochon, B. Robert Franza, and Ruedi Aebersold. "Correlation between Protein and mRNA Abundance in Yeast." Molecular and Cellular Biology 19, no. 3 (1999): 1720–30. http://dx.doi.org/10.1128/mcb.19.3.1720.

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ABSTRACT We have determined the relationship between mRNA and protein expression levels for selected genes expressed in the yeastSaccharomyces cerevisiae growing at mid-log phase. The proteins contained in total yeast cell lysate were separated by high-resolution two-dimensional (2D) gel electrophoresis. Over 150 protein spots were excised and identified by capillary liquid chromatography-tandem mass spectrometry (LC-MS/MS). Protein spots were quantified by metabolic labeling and scintillation counting. Corresponding mRNA levels were calculated from serial analysis of gene expression (SAGE) fr

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11

Chen, Xu-Qiao, Carlos A. Barrero, Rodrigo Vasquez-Del Carpio, et al. "Posiphen Reduces the Levels of Huntingtin Protein through Translation Suppression." Pharmaceutics 13, no. 12 (2021): 2109. http://dx.doi.org/10.3390/pharmaceutics13122109.

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Posiphen tartrate (Posiphen) is an orally available small molecule that targets a conserved regulatory element in the mRNAs of amyloid precursor protein (APP) and α-synuclein (αSYN) and inhibits their translation. APP and αSYN can cause neurodegeneration when their aggregates induce neurotoxicity. Therefore, Posiphen is a promising drug candidate for neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease. Posiphen’s safety has been demonstrated in three independent phase I clinical trials. Moreover, in a proof of concept study, Posiphen lowered neurotoxic proteins an

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12

Udy, Dylan B., and Robert K. Bradley. "Nonsense-mediated mRNA decay uses complementary mechanisms to suppress mRNA and protein accumulation." Life Science Alliance 5, no. 3 (2021): e202101217. http://dx.doi.org/10.26508/lsa.202101217.

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Nonsense-mediated mRNA decay (NMD) is an essential, highly conserved quality control pathway that detects and degrades mRNAs containing premature termination codons. Although the essentiality of NMD is frequently ascribed to its prevention of truncated protein accumulation, the extent to which NMD actually suppresses proteins encoded by NMD-sensitive transcripts is less well-understood than NMD-mediated suppression of mRNA. Here, we describe a reporter system that permits accurate quantification of both mRNA and protein levels via stable integration of paired reporters encoding NMD-sensitive a

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13

Huang, S., and J. W. Hershey. "Translational initiation factor expression and ribosomal protein gene expression are repressed coordinately but by different mechanisms in murine lymphosarcoma cells treated with glucocorticoids." Molecular and Cellular Biology 9, no. 9 (1989): 3679–84. http://dx.doi.org/10.1128/mcb.9.9.3679-3684.1989.

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P1798 murine lymphosarcoma cells cease to proliferate upon exposure to 10(-7) M dexamethasone and exhibit a dramatic inhibition of rRNA and ribosomal protein synthesis (O. Meyuhas, E. Thompson, Jr., and R. P. Perry, Mol. Cell Biol. 7:2691-2699, 1987). These workers demonstrated that ribosomal protein synthesis is regulated primarily at the level of translation, since dexamethasone did not alter mRNA levels but shifted the mRNAs from active polysomes into inactive messenger ribonucleoproteins. We have examined the effects of dexamethasone on the biosynthesis of initiation factor proteins in the

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14

Huang, S., and J. W. Hershey. "Translational initiation factor expression and ribosomal protein gene expression are repressed coordinately but by different mechanisms in murine lymphosarcoma cells treated with glucocorticoids." Molecular and Cellular Biology 9, no. 9 (1989): 3679–84. http://dx.doi.org/10.1128/mcb.9.9.3679.

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P1798 murine lymphosarcoma cells cease to proliferate upon exposure to 10(-7) M dexamethasone and exhibit a dramatic inhibition of rRNA and ribosomal protein synthesis (O. Meyuhas, E. Thompson, Jr., and R. P. Perry, Mol. Cell Biol. 7:2691-2699, 1987). These workers demonstrated that ribosomal protein synthesis is regulated primarily at the level of translation, since dexamethasone did not alter mRNA levels but shifted the mRNAs from active polysomes into inactive messenger ribonucleoproteins. We have examined the effects of dexamethasone on the biosynthesis of initiation factor proteins in the

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15

Bishola Tshitenge, Tania, and Christine Clayton. "Interactions of the Trypanosoma brucei brucei zinc-finger-domain protein ZC3H28." Parasitology 149, no. 3 (2021): 356–70. http://dx.doi.org/10.1017/s003118202100189x.

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AbstractIn Trypanosoma brucei and related Kinetoplastids, regulation of gene expression occurs mostly post-transcriptionally, and RNA-binding proteins play a critical role in the regulation of mRNA and protein abundance. Trypanosoma brucei ZC3H28 is a 114 KDa cytoplasmic mRNA-binding protein with a single C(x)7C(x)5C(x)sH zinc finger at the C-terminus and numerous proline-, histidine- or glutamine-rich regions. ZC3H28 is essential for normal bloodstream-form trypanosome growth, and when tethered to a reporter mRNA, ZC3H28 increased reporter mRNA and protein levels. Purification of N-terminally

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16

Esser, K. A., and E. C. Hardeman. "Changes in contractile protein mRNA accumulation in response to spaceflight." American Journal of Physiology-Cell Physiology 268, no. 2 (1995): C466—C471. http://dx.doi.org/10.1152/ajpcell.1995.268.2.c466.

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Ten rats were exposed to 9 days of zero gravity aboard the National Aeronautics and Space Administration SLS-1 space mission (June 1991). Levels of fast and slow isoform mRNAs from six contractile protein gene families were quantified in the flight soleus and extensor digitorum longus (EDL) muscles. The gene families studied were myosin light chain-1 (MLC-1), myosin light chain-2 (MLC-2), troponin (Tn) T, TnI, TnC, and tropomyosin. In the EDL muscle there was no change in slow mRNA levels with a general increase in fast mRNA levels from 23 to 232%. Changes in slow mRNA levels were seen in the

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17

Ševaljević, L., S. Ivanović-Matić, M. Petrović, M. Glibetić, D. Pantelić, and G. Poznanović. "Regulation of plasma acute-phase protein and albumin levels in the liver of scalded rats." Biochemical Journal 258, no. 3 (1989): 663–68. http://dx.doi.org/10.1042/bj2580663.

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At 12 h after scalding of rats a doubling of the hepatocyte nuclear DNA content, which arose from the presence of additional complete genomes and not from amplification of genes coding for the major acute-phase proteins or albumin, was observed. Examination of relative transcription rates per control DNA mass revealed that alpha 1-acid-glycoprotein and cysteine-proteinase-inhibitor genes remained constitutive, alpha- and gamma-fibrinogen and haptoglobin genes underwent transcriptional activation for 290 and 339% respectively, whereas the relative transcription rate of albumin decreased to 65%

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18

LI, Ying, Ulrike MENDE, Carol LEWIS та Eva J. NEER. "Maintenance of cellular levels of G-proteins: different efficiencies of αs and αo synthesis in GH3 cells". Biochemical Journal 318, № 3 (1996): 1071–77. http://dx.doi.org/10.1042/bj3181071.

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G-proteins couple membrane-bound receptors to intracellular effectors. Each cell has a characteristic complement of G-protein α, β and γ subunits that partly determines the cell's response to external signals. Very little is known about the mechanisms that set and maintain cellular levels of G-proteins or about potential points of regulation. We have assayed the steady-state levels of mRNA and protein for two types of G-protein subunits, αs and αo, in rat brain, heart and GH3 cells, and found that in all these cases, it takes 9- to 20-fold more mRNA to produce a given amount of αs protein than

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19

Tokuoka, Masafumi, Mizuki Tanaka, Kazuhisa Ono, Shinobu Takagi, Takahiro Shintani, and Katsuya Gomi. "Codon Optimization Increases Steady-State mRNA Levels in Aspergillus oryzae Heterologous Gene Expression." Applied and Environmental Microbiology 74, no. 21 (2008): 6538–46. http://dx.doi.org/10.1128/aem.01354-08.

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ABSTRACT We investigated the effect of codon optimization on the expression levels of heterologous proteins in Aspergillus oryzae, using the mite allergen Der f 7 as a model protein. A codon-optimized Der f 7 gene was synthesized according to the frequency of codon usage in A. oryzae by recursive PCR. Both native and optimized Der f 7 genes were expressed under the control of a high-level-expression promoter with their own signal peptides or in a fusion construct with A. oryzae glucoamylase (GlaA). Codon optimization markedly increased protein and mRNA production levels in both nonfused and Gl

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20

Chen, Chuan, Xu Zhang, Fei Shang, Haipeng Sun, Baolin Sun, and Ting Xue. "The Staphylococcus aureus Protein-Coding GenegdpSModulatessarSExpression via mRNA-mRNA Interaction." Infection and Immunity 83, no. 8 (2015): 3302–10. http://dx.doi.org/10.1128/iai.00159-15.

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Staphylococcus aureusis an important Gram-positive pathogen responsible for numerous diseases ranging from localized skin infections to life-threatening systemic infections. The virulence ofS. aureusis essentially determined by a wide spectrum of factors, including cell wall-associated proteins and secreted toxins that are precisely controlled in response to environmental changes. GGDEF domain protein fromStaphylococcus(GdpS) is the only conserved staphylococcal GGDEF domain protein that is involved not in c-di-GMP synthesis but in the virulence regulation ofS. aureusNCTC8325. Our previous stu

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21

BAL ALBAYRAK, Merve Gülsen, Sevinc YANAR, Murat KASAP, and Gürler AKPINAR. "ACE2 ve TMPRSS2 Genlerinin Farklı Hücre Hatlarındaki İfade Düzeyleri." Acta Medica Nicomedia 6, no. 2 (2023): 260–68. http://dx.doi.org/10.53446/actamednicomedia.1253701.

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Objective: ACE2 and TMPRSS2 proteins have received increased attention gained emphasis together with the pandemic COVID-19. These proteins have roles in respiratory and hypertension disorders as well as cardiovascular and renal diseases. The objective of this work was to examine the mRNA and protein levels of ACE2 and TMPRSS2 in cell lines derived from various tissue origins. Methods: After the growth of 14 different cell lines, protein and mRNA were isolated from the cell pellets. The amounts of mRNAs and proteins were then determined and quantified using RT-PCR and ELISA. Results

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22

Kedersha, N., and P. Anderson. "Stress granules: sites of mRNA triage that regulate mRNA stability and translatability." Biochemical Society Transactions 30, no. 6 (2002): 963–69. http://dx.doi.org/10.1042/bst0300963.

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Mammalian stress granules (SGs) are cytoplasmic domains into which mRNAs are sorted dynamically in response to phosphorylation of eukaryotic initiation factor (eIF) 2α, a key regulatory step in translational initiation. The activation of one or more of the eIF2α kinases leads to SG assembly by decreasing the levels of eIF2-GTP-tRNAMet, the ternary complex that is normally required for loading the initiator methionine onto the 48 S preinitiation complex to begin translation. This stress-induced scarcity of eIF2-GTP-tRNAMet allows the RNA-binding proteins TIA-1 (T-cell internal antigen-1) and TI

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23

Ishikawa, Tomomoto, Keumsil Hwang, Deborah Lazzarino та Patricia L. Morris. "Sertoli Cell Expression of Steroidogenic Acute Regulatory Protein-Related Lipid Transfer 1 and 5 Domain-Containing Proteins and Sterol Regulatory Element Binding Protein-1 Are Interleukin-1β Regulated by Activation of c-Jun N-Terminal Kinase and Cyclooxygenase-2 and Cytokine Induction". Endocrinology 146, № 12 (2005): 5100–5111. http://dx.doi.org/10.1210/en.2005-0567.

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In testicular Sertoli cells, IL-1β regulates steroid, lactate, and transferrin secretion; although each influences germ cell development and spermatogenesis, little is known about the signaling mechanisms involved. In other cell types, IL-1β potently induces reactive oxygen species and/or cyclooxygenase-2 (COX-2). In contrast, in Sertoli cells, IL-1β does not generate reactive oxygen species, but rapidly phosphorylates c-Jun-NH2-terminal kinase (JNK), but not p44/42 or p38 MAPK. Phosphorylated JNK stimulates COX-2 activity, mediating the expression of ILs and steroidogenic acute regulatory (St

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24

Roth, Kelly M., Maria K. Wolf, Marie Rossi, and J. Scott Butler. "The Nuclear Exosome Contributes to Autogenous Control of NAB2 mRNA Levels." Molecular and Cellular Biology 25, no. 5 (2005): 1577–85. http://dx.doi.org/10.1128/mcb.25.5.1577-1585.2005.

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ABSTRACT The RNA-processing exosome is a complex of riboexonucleases required for 3′-end formation of some noncoding RNAs and for the degradation of mRNAs in eukaryotes. The nuclear form of the exosome functions in an mRNA surveillance pathway that retains and degrades improperly processed precursor mRNAs within the nucleus. We report here that the nuclear exosome controls the level of NAB2 mRNA, encoding the nuclear poly(A)+-RNA-binding protein Nab2p. Mutations affecting the activity of the nuclear, but not the cytoplasmic, exosome cause an increase in the amount of NAB2 mRNA. Cis- and trans-

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25

Fortelny, Nikolaus, Christopher M. Overall, Paul Pavlidis, and Gabriela V. Cohen Freue. "Can we predict protein from mRNA levels?" Nature 547, no. 7664 (2017): E19—E20. http://dx.doi.org/10.1038/nature22293.

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Watkins, David C., Peter J. Rapiejko, Manuel Ros, Hsien-yu Wang, and Craig C. Malbon. "G-Protein mRNA levels during adipocyte differentiation." Biochemical and Biophysical Research Communications 165, no. 3 (1989): 929–34. http://dx.doi.org/10.1016/0006-291x(89)92692-2.

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Strowski, M. Z., G. Sparmann, H. Weber, et al. "Caerulein pancreatitis increases mRNA but reduces protein levels of rat pancreatic heat shock proteins." American Journal of Physiology-Gastrointestinal and Liver Physiology 273, no. 4 (1997): G937—G945. http://dx.doi.org/10.1152/ajpgi.1997.273.4.g937.

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We have recently reported that preconditioning through hyperthermia induces expression of pancreatic heat shock proteins (HSPs) and protects against caerulein pancreatitis. Here, we investigate caerulein-mediated effects on pancreatic HSPs without prior hyperthermia. Caerulein time and dose dependently increased pancreatic mRNA levels of the constitutive isoform of HSP70 (HSC70). However, pancreatic HSC70 protein levels were decreased, as were HSP60 protein levels. Also, in contrast to hyperthermia preconditioning, caerulein did not induce measurable levels of mRNA or protein of the inducible

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Lopez, Nelson, John Halladay, William Walter, and Elizabeth A. Craig. "SSB, Encoding a Ribosome-Associated Chaperone, Is Coordinately Regulated with Ribosomal Protein Genes." Journal of Bacteriology 181, no. 10 (1999): 3136–43. http://dx.doi.org/10.1128/jb.181.10.3136-3143.1999.

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ABSTRACT Genes encoding ribosomal proteins and other components of the translational apparatus are coregulated to efficiently adjust the protein synthetic capacity of the cell. Ssb, a Saccharomyces cerevisiae Hsp70 cytosolic molecular chaperone, is associated with the ribosome-nascent chain complex. To determine whether this chaperone is coregulated with ribosomal proteins, we studied the mRNA regulation of SSB under several environmental conditions. Ssb and the ribosomal protein rpL5 mRNAs were up-regulated upon carbon upshift and down-regulated upon amino acid limitation, unlike the mRNA of

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Schoolwerth, A. C., P. A. deBoer, A. F. Moorman, and W. H. Lamers. "Changes in mRNAs for enzymes of glutamine metabolism in kidney and liver during ammonium chloride acidosis." American Journal of Physiology-Renal Physiology 267, no. 3 (1994): F400—F406. http://dx.doi.org/10.1152/ajprenal.1994.267.3.f400.

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Changes in protein and mRNAs for enzymes of glutamine metabolism were determined in rat kidney cortex at different times after induction of NH4Cl acidosis. After NH4Cl, phosphoenolpyruvate carboxykinase (PEPCK) mRNA increased 16-fold by 10 h (P < 0.05) and then returned to control levels by 30 h. In situ hybridization (ISH) showed that PEPCK mRNA was confined to medullary rays; after NH4Cl, expression of PEPCK expanded throughout the cortex, reaching a maximal intensity at 10 h. Phosphate-dependent glutaminase (PDG) and glutamate dehydrogenase (GDH) mRNAs increased 8- and 2.6-fold, respecti

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Yeo, Seung Geun, Sung Jong Lee, Ji Woo Lee, Sujung Oh, and Dong Choon Park. "Levels of endoplasmic reticulum stress-related mRNA in peritoneal fluid of patients with endometriosis or gynaecological cancer." Journal of International Medical Research 49, no. 12 (2021): 030006052110653. http://dx.doi.org/10.1177/03000605211065376.

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Objective To compare the levels of endoplasmic reticulum (ER) stress-associated mRNAs and the clinical characteristics of patients with endometriosis or gynaecological cancer. Methods This prospective study obtained intraperitoneal fluid samples from female patients that underwent surgery. The levels of ER stress mRNAs in the peritoneal fluid, including C/EBP-homologous protein (CHOP), X-box binding protein 1 (sXBP1), activating transcription factor 6 (ATF6), immunoglobulin heavy chain-binding protein (BiP), inositol-requiring enzyme 1α (IRE1α) and protein kinase RNA-like endoplasmic reticulum

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Majumder, Mithu, Ibrahim Yaman, Francesca Gaccioli, et al. "The hnRNA-Binding Proteins hnRNP L and PTB Are Required for Efficient Translation of the Cat-1 Arginine/Lysine Transporter mRNA during Amino Acid Starvation." Molecular and Cellular Biology 29, no. 10 (2009): 2899–912. http://dx.doi.org/10.1128/mcb.01774-08.

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ABSTRACT The response to amino acid starvation involves the global decrease of protein synthesis and an increase in the translation of some mRNAs that contain an internal ribosome entry site (IRES). It was previously shown that translation of the mRNA for the arginine/lysine amino acid transporter Cat-1 increases during amino acid starvation via a mechanism that utilizes an IRES in the 5′ untranslated region of the Cat-1 mRNA. It is shown here that polypyrimidine tract binding protein (PTB) and an hnRNA binding protein, heterogeneous nuclear ribonucleoprotein L (hnRNP L), promote the efficient

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Wagner, Eric J., and William F. Marzluff. "ZFP100, a Component of the Active U7 snRNP Limiting for Histone Pre-mRNA Processing, Is Required for Entry into S Phase." Molecular and Cellular Biology 26, no. 17 (2006): 6702–12. http://dx.doi.org/10.1128/mcb.00391-06.

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ABSTRACT Metazoan replication-dependent histone mRNAs are the only eukaryotic mRNAs that are not polyadenylated. The cleavage of histone pre-mRNA to form the unique 3′ end requires the U7 snRNP and the stem-loop binding protein (SLBP) that binds the 3′ end of histone mRNA. U7 snRNP contains three novel proteins, Lsm10 and Lsm11, which are part of the core U7 Sm complex, and ZFP100, a Zn finger protein that helps stabilize binding of the U7 snRNP to the histone pre-mRNA by interacting with the SLBP/pre-mRNA complex. Using a reporter gene that encodes a green fluorescent protein mRNA ending in a

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Avignon, A., M. L. Standaert, K. Yamada, H. Mischak, B. Spencer та R. V. Farese. "Insulin increases mRNA levels of protein kinase C-α and -β in rat adipocytes and protein kinase C-α, -β and -θ in rat skeletal muscle". Biochemical Journal 308, № 1 (1995): 181–87. http://dx.doi.org/10.1042/bj3080181.

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Effects of insulin of levels of mRNA encoding protein kinase C (PKC)-alpha, PKC-beta, PKC-epsilon and PKC-theta were examined by ribonuclease protection assay in primary cultures of rat adipocytes in vitro, and in rat adipose tissue and gastrocnemius muscle in vivo. In all cases, insulin increased the levels of PKC-alpha mRNA and PKC-beta mRNA, and, in muscle, insulin also increased the level of PKC-theta mRNA. PKC-epsilon mRNA levels, on the other hand, were not altered significantly. Insulin also stimulated the apparent translocation of PKC-alpha, -beta, -epsilon and -theta, to the membrane

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Stallknecht, B., P. H. Andersen, J. Vinten, et al. "Effect of physical training on glucose transporter protein and mRNA levels in rat adipocytes." American Journal of Physiology-Endocrinology and Metabolism 265, no. 1 (1993): E128—E134. http://dx.doi.org/10.1152/ajpendo.1993.265.1.e128.

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Physical training increases insulin-stimulated glucose transport and the number of glucose transporters in adipocytes measured by cytochalasin B binding. In the present study we used immunoblotting to measure the abundance of two glucose transporters (GLUT-4, GLUT-1) in white adipocytes from trained rats. Furthermore, the abundance of the mRNAs for these proteins and glucose transport was measured. Rats were swim-trained for 10 wk, and adipocytes were isolated from epididymal fat pads. The amount of GLUT-4/adipocyte volume unit was significantly higher in trained animals compared with both age

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Laye, Matthew J., Thomas P. J. Solomon, Kristian Karstoft, Karin K. Pedersen, Susanne D. Nielsen, and Bente K. Pedersen. "Increased shelterin mRNA expression in peripheral blood mononuclear cells and skeletal muscle following an ultra-long-distance running event." Journal of Applied Physiology 112, no. 5 (2012): 773–81. http://dx.doi.org/10.1152/japplphysiol.00997.2011.

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Located at the end of chromosomes, telomeres are progressively shortened with each replication of DNA during aging. Integral to the regulation of telomere length is a group of proteins making up the shelterin complex, whose tissue-specific function during physiological stress is not well understood. In this study, we examine the mRNA and protein levels of proteins within and associated with the shelterin complex in subjects ( n = 8, mean age = 44 yr) who completed a physiological stress of seven marathons in 7 days. Twenty-two to 24 h after the last marathon, subjects had increased mRNA levels

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Castagnola, P., M. Gennari, R. Morello, et al. "Cartilage associated protein (CASP) is a novel developmentally regulated chick embryo protein." Journal of Cell Science 110, no. 12 (1997): 1351–59. http://dx.doi.org/10.1242/jcs.110.12.1351.

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A subtracted cDNA library was generated to identify cDNAs specific for chondrocyte mRNAs preferentially expressed at the hypertrophic stage with respect to early differentiation stages. The characterization of a cDNA isolated from this library that hybridizes with a 1.8 kb mRNA is described here. This mRNA is expressed at extremely low levels in dedifferentiated chondrocytes cultured in adherent conditions, at very low levels in differentiating chondrocytes and at very high levels in hypertrophic chondrocytes cultured in suspension conditions. In the developing chick embryo this mRNA is detect

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Yeh, Kwo-Yih, Mary Yeh, and Jonathan Glass. "Expression of intestinal brush-border membrane hydrolases and ferritin after segmental ischemia-reperfusion in rats." American Journal of Physiology-Gastrointestinal and Liver Physiology 275, no. 3 (1998): G572—G583. http://dx.doi.org/10.1152/ajpgi.1998.275.3.g572.

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Jejunal expression of three brush-border membrane (BBM) enzymes, intestinal alkaline phosphatase (IAP), lactose-phlorizin hydrolase (LPH), and sucrase-isomaltase (SI), and a cytosolic protein, ferritin (Ft), was investigated after transient segmental ischemia-reperfusion (I/R). I/R reduced mucosal IAP, LPH, and SI mRNAs to 36%, 11%, and 38% of normal jejunal levels after 3 h of reperfusion and to 22%, 8%, and 51% of normal jejunal levels after 6 h of reperfusion, respectively. Intriguingly, in the internal control jejunum IAP and LPH mRNAs also decreased significantly. LPH and SI mRNA rapidly

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Milland, J., A. Tsykin, T. Thomas, A. R. Aldred, T. Cole, and G. Schreiber. "Gene expression in regenerating and acute-phase rat liver." American Journal of Physiology-Gastrointestinal and Liver Physiology 259, no. 3 (1990): G340—G347. http://dx.doi.org/10.1152/ajpgi.1990.259.3.g340.

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The integration of growth and the acute-phase response is investigated by comparing the mRNA levels in rat liver during acute inflammation with those after partial hepatectomy. Northern analysis is carried out for the mRNAs for thiostatin, alpha 2-macroglobulin, alpha 1-antitrypsin, inter-alpha-trypsin inhibitor subunit 1, haptoglobin, ceruloplasmin, transferrin, vitamin D-binding protein, alpha 1-acid glycoprotein, beta-fibrinogen, apolipoproteins A-IV and E, albumin, transthyretin, alpha 2-HS-glycoprotein, retinol-binding protein, beta-tubulin, c-myc protooncogene, glyceraldehyde-3-phosphate

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39

Hu, S., Y. Li, J. Wang, et al. "Human Saliva Proteome and Transcriptome." Journal of Dental Research 85, no. 12 (2006): 1129–33. http://dx.doi.org/10.1177/154405910608501212.

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This paper tests the hypothesis that salivary proteins and their counterpart mRNAs co-exist in human whole saliva. Global profiling of human saliva proteomes and transcriptomes by mass spectrometry (MS) and expression microarray technologies, respectively, revealed many similarities between saliva proteins and mRNAs. Of the function-known proteins identified in saliva, from 61 to 70% were also found present as mRNA transcripts. For genes not detected at both protein and mRNA levels, we made further efforts to determine if the counterpart is present. Of 19 selected genes detected only at the pr

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Duffy, Carol, Ekaette F. Mbong, and Joel D. Baines. "VP22 of Herpes Simplex Virus 1 Promotes Protein Synthesis at Late Times in Infection and Accumulation of a Subset of Viral mRNAs at Early Times in Infection." Journal of Virology 83, no. 2 (2008): 1009–17. http://dx.doi.org/10.1128/jvi.02245-07.

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ABSTRACT VP22, encoded by the UL49 gene, is one of the most abundant proteins of the herpes simplex virus 1 (HSV-1) tegument. In the present study we show VP22 is required for optimal protein synthesis at late times in infection. Specifically, in the absence of VP22, viral proteins accumulated to wild-type levels until ∼6 h postinfection. At that time, ongoing synthesis of most viral proteins dramatically decreased in the absence of VP22, whereas protein stability was not affected. Of the individual proteins we assayed, VP22 was required for optimal synthesis of the late viral proteins gE and

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Kawase, Atsushi, Akira Kazaoka, Rei Yamamoto, Risa Minakata, Hiroaki Shimada, and Masahiro Iwaki. "Changes in Transporters and Metabolizing Enzymes in the Livers of Rats with Bile Duct Ligation." Journal of Pharmacy & Pharmaceutical Sciences 22 (September 19, 2019): 457–65. http://dx.doi.org/10.18433/jpps30637.

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Purpose: Bile duct ligation (BDL) in experimental animals is widely used as an animal model of liver cholestasis and fibrosis. The transcriptional process and plasma membrane localization of transporters are regulated by nuclear receptors and scaffold proteins, respectively. However, the detailed changes of these factors in the livers of BDL rats remain unclear. To clarify the effects of BDL on the levels of transporters and metabolizing enzymes, nuclear receptors, and scaffold proteins, we investigated changes in mRNA and protein levels of livers from BDL rats. Methods: Membrane proteins and

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Lan, Ping, Wenfeng Li, and Wolfgang Schmidt. "Complementary Proteome and Transcriptome Profiling in Phosphate-deficient Arabidopsis Roots Reveals Multiple Levels of Gene Regulation." Molecular & Cellular Proteomics 11, no. 11 (2012): 1156–66. http://dx.doi.org/10.1074/mcp.m112.020461.

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Phosphate (Pi) deficiency impairs plant growth and productivity in many agricultural ecosystems, causing severe reductions in crop yield. To uncover novel aspects in acclimation to Pi starvation, we investigated the correlation between Pi deficiency-induced changes in transcriptome and proteome profiles in Arabidopsis roots. Using exhaustive tandem mass spectrometry-based shotgun proteomics and whole-genome RNA sequencing to generate a nearly complete catalog of expressed mRNAs and proteins, we reliably identified 13,298 proteins and 24,591 transcripts, subsets of 356 proteins and 3106 mRNAs w

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Dykeman, Eric C. "Modelling ribosome kinetics and translational control on dynamic mRNA." PLOS Computational Biology 19, no. 1 (2023): e1010870. http://dx.doi.org/10.1371/journal.pcbi.1010870.

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The control of protein synthesis and the overall levels of various proteins in the cell is critical for achieving homoeostasis. Regulation of protein levels can occur at the transcriptional level, where the total number of messenger RNAs in the overall transcriptome are controlled, or at the translational level, where interactions of proteins and ribosomes with the messenger RNA determine protein translational efficiency. Although transcriptional control of mRNA levels is the most commonly used regulatory control mechanism in cells, positive-sense single-stranded RNA viruses often utilise tran

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Hartmann, Claudia, Corinna Benz, Stefanie Brems, et al. "Small Trypanosome RNA-Binding Proteins TbUBP1 and TbUBP2 Influence Expression of F-Box Protein mRNAs in Bloodstream Trypanosomes." Eukaryotic Cell 6, no. 11 (2007): 1964–78. http://dx.doi.org/10.1128/ec.00279-07.

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ABSTRACT In the African trypanosome Trypanosoma brucei nearly all control of gene expression is posttranscriptional; sequences in the 3′-untranslated regions of mRNAs determine the steady-state mRNA levels by regulation of RNA turnover. Here we investigate the roles of two related proteins, TbUBP1 and TbUBP2, containing a single RNA recognition motif, in trypanosome gene expression. TbUBP1 and TbUBP2 are in the cytoplasm and nucleus, comprise ca. 0.1% of the total protein, and are not associated with polysomes or RNA degradation enzymes. Overexpression of TbUBP2 upregulated the levels of sever

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Warringer, Jonas, Malin Hult, Sergi Regot, Francesc Posas, and Per Sunnerhagen. "The HOG Pathway Dictates the Short-Term Translational Response after Hyperosmotic Shock." Molecular Biology of the Cell 21, no. 17 (2010): 3080–92. http://dx.doi.org/10.1091/mbc.e10-01-0006.

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Cellular responses to environmental changes occur on different levels. We investigated the translational response of yeast cells after mild hyperosmotic shock by isolating mRNA associated with multiple ribosomes (polysomes) followed by array analysis. Globally, recruitment of preexisting mRNAs to ribosomes (translational response) is faster than the transcriptional response. Specific functional groups of mRNAs are recruited to ribosomes without any corresponding increase in total mRNA. Among mRNAs under strong translational up-regulation upon shock, transcripts encoding membrane-bound proteins

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Dominguez, J. H., C. C. Hale, and M. Qulali. "Studies of renal injury. I. Gentamicin toxicity and expression of basolateral transporters." American Journal of Physiology-Renal Physiology 270, no. 2 (1996): F245—F253. http://dx.doi.org/10.1152/ajprenal.1996.270.2.f245.

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Gentamicin nephrotoxicity may arise in part from alterations in the expression of genes critical for renal proximal tubule metabolism. We tested the hypothesis that gentamicin suppressed the gene expression of the Na+/Ca2+ exchanger (NaCaX), glucose transporter 1 (GLUT1) and alpha 1-subunit of Na(+)-K(+)-ATPase (alpha 1-NKA) in renal tubules. The products of these genes mediate Na(+)-dependent Ca2+ efflux, glucose efflux and influx, and ATP-dependent Na+ efflux across tubular basolateral membranes, respectively. After 10 days of gentamicin intoxication (40 mg/kg ip, twice daily), levels of mRN

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Eble, D. M., and F. G. Spinale. "Contractile and cytoskeletal content, structure, and mRNA levels with tachycardia-induced cardiomyopathy." American Journal of Physiology-Heart and Circulatory Physiology 268, no. 6 (1995): H2426—H2439. http://dx.doi.org/10.1152/ajpheart.1995.268.6.h2426.

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Chronic supraventricular tachycardia (SVT)-induced cardiomyopathy is associated with left ventricular (LV) dilatation, increased wall stress, neurohormonal activation, and no change in LV mass. To determine mechanisms for changes in LV geometry and function with SVT cardiomyopathy, LV and myocyte function, contractile protein content and mRNA levels, and cytoskeletal protein structure and mRNA levels were examined in 12 pigs with SVT cardiomyopathy (paced at 240 beats/min for 3 wk) and in 12 controls. With SVT cardiomyopathy, LV fractional shortening fell by 61%, and end-diastolic dimension in

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Li, Jiawei, Yi Zhang, Cheng Yang, and Ruiming Rong. "Discrepant mRNA and Protein Expression in Immune Cells." Current Genomics 21, no. 8 (2020): 560–63. http://dx.doi.org/10.2174/1389202921999200716103758.

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With the development of single-cell mRNA sequencing (scRNA-seq), researchers have attempted to identify new methods for performing in-depth studies of immune cells. However, the discrepancies between the mRNA levels and the levels of surface proteins have confused many researchers. Here, we report a significant and interesting phenomenon in which the mRNA and protein expression levels were mismatched in immune cells. We concluded that scRNA-seq should be combined with other sequencing methods in single-cell studies (e.g., CITE-seq). The simultaneous assessment of both mRNA and protein expressi

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Banik, Jewel, Katherine Bronson, Gwen Childs, et al. "OR08-2 Functional Association of the Stem Cell Protein Musashi With LSM14B in Control of mRNA Translation." Journal of the Endocrine Society 6, Supplement_1 (2022): A452—A453. http://dx.doi.org/10.1210/jendso/bvac150.941.

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Abstract The Musashi RNA-binding protein functions as a gatekeeper of cell maturation and maintains stem cell plasticity by regulating the translation of target mRNAs. The adult anterior pituitary tissue expresses a high level of Musashi and also demonstatres a high level of cell plasticity, indicating a Musashi-dependent function in maintaining endocrine homeostasis in the anterior pituitary. Towards understanding the mechanism(s) by which Musashi functions to control cell plasticity, we have identified co-associated proteins necessary for Musashi function. The two Musashi isoforms (Musashi1

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D'Agostino, D. M., B. K. Felber, J. E. Harrison, and G. N. Pavlakis. "The Rev protein of human immunodeficiency virus type 1 promotes polysomal association and translation of gag/pol and vpu/env mRNAs." Molecular and Cellular Biology 12, no. 3 (1992): 1375–86. http://dx.doi.org/10.1128/mcb.12.3.1375-1386.1992.

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Biochemical examination of the Rev-dependent expression of gag mRNAs produced from gag-Rev-responsive element (RRE) expression plasmids showed a large discrepancy between the level of cytoplasmic gag mRNA and the produced Gag protein. Significant levels of the mRNA produced in the absence of Rev were localized in the cytoplasm, while very low levels of Gag protein were produced. In the presence of Rev, the levels of mRNA increased by 4- to 16-fold, while the Gag protein production increased by 800-fold. These findings indicated that in addition to promoting nucleus-to-cytoplasm transport, Rev

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