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1

Silva, Maria José Cavalcante da, Sebastião Ferreira Palmeira Junior, Kleber Fortes Junior, et al. "IAA production of indigenous isolate of plant growth promoting rhizobacteria in the presence of tryptophan." March 2020, no. 14(03):2020 (March 20, 2020): 537–44. http://dx.doi.org/10.21475/ajcs.20.14.03.p2239.

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The plant growth-promoting rhizobacteria (PGPRBs) is an interesting way to promote increased vegetable production. Here, we aimed to isolate, identify, and characterize PGPRBs by using biochemical tests, sequencing of 16S ribossomal DNA, in vitro and screening for indoleacetic acid production. The isolates were identified through VITEK® 2 Compact equipment, which is an automated system. We identified microorganisms such as Alcaligenes faecalis sp. faecalis, Pseudomonas putida, Proteus vulgaris, Providencia rettgeri, Serratia marcescens e Myroides sp. by performing vitek 2 biochemical tests. Th
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Praveen, Kumar S. V., John Indulekha, K. Valarmathy, and B. K. Manjunatha. "Statistical Optimization of Crude Oil Degradation using Bacterial Consortium and their Enzymes." Research Journal of Biotechnology 18, no. 11 (2023): 115–24. http://dx.doi.org/10.25303/1811rjbt01150124.

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The present study investigated the application of central composite design in enhanced crude oil remediation using bacterial consortium. The bacterial strains viz. KG-2, SMG-8, NR-3 and OK-6(2) isolated from soil were selected for the crude oil utilization studies. The strains were identified as Pseudomonas stutzeri-KX344913, P. stutzeri-KX289657, Providencia rettgeri-KX289656 and P. rettgeri-KX344914 respectively using 16s rDNA sequence. The preliminary degradation was done using the DCPIP redox-indicator and further degradation was conducted by using Response Surface Methodology (RSM) and th
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Ayyal Al‐Gburi, Nagham Mohammed. "Isolation and Molecular Identification and Antimicrobial Susceptibility of Providencia spp. from Raw Cow’s Milk in Baghdad, Iraq." Veterinary Medicine International 2020 (November 18, 2020): 1–6. http://dx.doi.org/10.1155/2020/8874747.

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A total of sixty raw milk samples were collected from (street vendors and shops) from Baghdad city, Iraq. The samples were inoculated into peptone water and, then, subcultured onto McConkey agar and Blood agar. Identification of isolates was confirmed by microscopic examination, cultural characteristic, biochemical tests, Vitek (VITEK®2 system), and Biolog GN substrate reactions followed by 16S rRNA and specific genes sequencing. Of 60 raw cow’s milk samples, Providencia spp. were identified only in 4 samples (6.67%) and P. rettgeri was the most common, 2/4 (50%), followed by P. stuartii and P
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Singh, N. K., D. K. Verma, A. K. Upadhyay, and U. N. Rai. "Isolation and Characterization of Arsenic Tolerant Bacteria Collected from Arsenic Contaminated Site of West Bengal, India." INTERNATIONAL JOURNAL OF PLANT AND ENVIRONMENT 5, no. 01 (2019): 57–62. http://dx.doi.org/10.18811/ijpen.v5i01.10.

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In the present study, arsenic tolerant bacteria were isolated and characterized from rhizospheric soil of rice plant growing in the arsenic contaminated area of West Bengal, India. Among 31 bacterial isolates, nine isolates were showed tolerance at higher concentration of arsenate (As V; 20000 mg mL-1). Selected arsenic tolerance bacterial isolates were characterized based on biochemical analysis, partial 16S rDNA gene sequencing and phylogenetic analysis. Results revealed that selected arsenic tolerant bacterial strains were lavished at 370C and neutral pH 7 and showed a positive response for
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MRAMBA, F., A. BROCE, and L. ZUREK. "Isolation of Enterobacter sakazakii from Stable Flies, Stomoxys calcitrans L. (Diptera: Muscidae)†." Journal of Food Protection 69, no. 3 (2006): 671–73. http://dx.doi.org/10.4315/0362-028x-69.3.671.

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Enterobacter sakazakii is an opportunistic foodborne pathogen that causes meningitis, enterocolitis, and sepsis, primarily in immunocompromised infants. Previously, it was suggested that stable flies, Stomoxys calcitrans, were a vector or reservoir of this pathogen. In our study, by means of a culturing approach combined with 16S rDNA PCR–restriction fragment length polymorphism genotyping and sequencing, we screened 928 individual stable flies collected in Kansas and Florida. Two stable flies (0.2%) were positive for E. sakazakii. In addition, 411 (44%) stable flies carried bacteria-forming r
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6

Rabhi, Nour El Houda, Hafsa Cherif-Silini, Allaoua Silini, et al. "Alleviation of Salt Stress via Habitat-Adapted Symbiosis." Forests 13, no. 4 (2022): 586. http://dx.doi.org/10.3390/f13040586.

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Halotolerant plant-growth-promoting rhizobacteria (PGPR) could not only promote plant growth, but also help in counteracting the detrimental effects of salt stress. In the present study, a total of 76 bacteria were isolated from the rhizosphere, non-rhizospheric soil and endophytes of the halophyte Salsola tetrandra, collected from natural saline soils in Algeria. Phylogenetic analysis based on the 16S rDNA sequence of Gram-negative bacteria (n = 51) identified, showed seventeen representative isolates grouped into four genera (Pseudomonas, Acinetobacter, Enterobacter, and Providencia). These
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Boumerdassi, Hanane, Lydia Neïla Djouadi, Abderrahmane Hambli, Marie-Laure Fardeau, Hadda-Imen Ouzari, and Farida Nateche. "Physicochemical and Microbiological Water Quality Assessment of a Northwestern Algerian Dam: Detection of Ichtyopathogenic Bacteria." Polish Journal of Microbiology 72, no. 2 (2023): 187–98. http://dx.doi.org/10.33073/pjm-2023-020.

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Abstract Freshwater fish are often exposed to threats from anthropogenic or natural origins, such as pathogenic or opportunistic microorganisms responsible for a broad range of severe infections. In this study, we aimed to assess this microbiological threat to fish in an Algerian northwestern dam Sekkak (Tlemcen) by evaluating the diversity of ichtyopathogenic bacteria. In order to determine the water quality, physicochemical analyses of the dam water were carried out in situ. Ichtyopathogenic bacteria were isolated on selective media and identified by API galleries and molecular techniques (P
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8

Cochrane, Christine A., Karen Freeman, Emma Woods, Sarah Welsby, and Steven L. Percival. "Biofilm evidence and the microbial diversity of horse wounds." Canadian Journal of Microbiology 55, no. 2 (2009): 197–202. http://dx.doi.org/10.1139/w08-115.

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Evidence of biofilms in human chronic wounds are thought to be responsible for preventing healing in a timely manner. However, biofilm evidence in horse wounds has not yet been documented. Consequently, this study aimed to determine whether biofilms could be detected in wounds, and to investigate the microbiology of chronic wounds in horses. Prior to analysis, wound surfaces were irrigated with 5 mL of sterile saline to remove debris. All wounds were swabbed twice (1 cm2area) using sterile cotton-tipped swabs. In addition to this, 2 tissue biopsies were taken to investigate evidence of biofilm
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9

Adesoji, Ayodele T., and Adeniyi A. Ogunjobi. "Identification of Aminoglycoside Resistance Genes From Bacteria Isolated From Selected Municipal Drinking Water Distribution Systems in Southwestern Nigeria." Infection Control & Hospital Epidemiology 41, S1 (2020): s255. http://dx.doi.org/10.1017/ice.2020.818.

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Background: Multidrug-resistant bacteria can lead to treatment failure, resulting in infectious diseases being transferred through nonpotable water. Aminoglycosides are an important class of antibiotics that are abused in Nigeria. Few studies have investigated aminoglycoside-modifying genes (AMGs) that are likely responsible for resistance in Nigeria bacteria isolates. Therefore, we aimed to characterize AMGs from isolates in drinking water distribution systems (DWDS) in southwestern Nigeria. Methods: Multidrug-resistant bacteria (n = 181) that had been previously characterized by 16S rDNA seq
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Trivedi, Mahendra Kumar, Shrikant Patil, Harish Shettigar, Khemraj Bairwa, and Snehasis Jana. "Effect of Biofield Treatment on Phenotypic and Genotypic Characteristic of Provindencia rettgeri." Molecular Biology 4, no. 3 (2015). https://doi.org/10.4172/2168-9547.1000129.

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Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence. The samples of P. rettgeri (ATCC 9250) were divided into three groups: Gr.I (control), Gr.II (treatment, revived), and Gr.III (treatment, lyophilized). The Gr.II an
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11

Trivedi, Mahendra. "Effect of biofield treatment on phenotypic and genotypic characteristic of Provindencia rettgeri." OMICS International, July 27, 2015. https://doi.org/10.5281/zenodo.813390.

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Providencia rettgeri (P. rettgeri) is a clinically significant Gram-negative bacterium of genus Providencia, and commonly associated with hospital-acquired infection like urinary tract infection (UTI), gastroenteritis, and ocular infections. Present study was designed to evaluate the effect of biofield treatment on P. rettgeri against antimicrobial susceptibility, biochemical reaction pattern, biotype number, and 16S rDNA sequence. The samples of P. rettgeri (ATCC 9250) were divided into three groups: Gr.I (control), Gr.II (treatment, revived), and Gr.III (treatment, lyophilized). The Gr.II an
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12

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella morganii." Advanced Techniques in Biology & Medicine 3, no. 3 (2015). https://doi.org/10.5281/zenodo.166862.

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Morganella morganii (M. morganii) is one of the important nosocomial pathogen associated with the urinary tract infections and bacteremia. The aim of this study was to evaluate the effect of Mr. Trivedi’s biofield energy treatment on M. morganii in the lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, biotype number and genotype. M. morganii cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 25829) number and stored according to the recommended storage protocols unt
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13

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, Gopal Nayak, Mayank Gangwar, and Snehasis Jana. "Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella morganii." Advanced Techniques in Biology & Medicine 3, no. 3 (2015). https://doi.org/10.5281/zenodo.49018.

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Morganella morganii (M. morganii) is one of the important nosocomial pathogen associated with the urinary tract infections and bacteremia. The aim of this study was to evaluate the effect of Mr. Trivedi’s biofield energy treatment on M. morganii in the lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, biotype number and genotype. M. morganii cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 25829) number and stored according to the recommended storage protocols unt
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14

Trivedi, Mahendra, Alice Branton, Dahryn Trivedi, and Gopal Nayak. "Antibiogram and Genotypic Analysis using 16S rDNA after Biofield Treatment on Morganella Morganii." OMICS International, September 17, 2015. https://doi.org/10.5281/zenodo.813410.

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Morganella morganii (M. morganii) is one of the important nosocomial pathogen associated with the urinary tract infections and bacteremia. The aim of this study was to evaluate the effect of Mr. Trivedi's biofield energy treatment on M. morganii in the lyophilized as well as revived state for antimicrobial susceptibility pattern, biochemical characteristics, biotype number and genotype. M. morganii cells were procured from MicroBioLogics Inc., USA in sealed packs bearing the American Type Culture Collection (ATCC 25829) number and stored according to the recommended storage protocols until nee
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15

Ganegoda, Sathya S., and Pathmalal M. Manage. "Isolation and identification of novel, native geosmin and 2-methylisoborneol degrading bacteria." Water Practice & Technology, February 26, 2025. https://doi.org/10.2166/wpt.2025.032.

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ABSTRACT Geosmin and 2-methylisoborneol (2-MIB) are compounds responsible for taste and odor issues in water, rendering drinking water unpalatable and prompting customer complaints. The present study aimed to identify geosmin and 2-MIB degrading bacteria. The Biolog MT2 assay was used to determine bacterial metabolism on geosmin and 2-MIB. Out of 100 and 23 native bacteria screened using the Biolog MT2 assay, 73 bacterial isolates tested positive. Degradation kinetics identified 10 bacteria capable of degrading both geosmin and 2-MIB. These bacteria were further characterized through 16S rDNA
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16

Mc Gann, Patrick, Matthew R. Geringer, Lindsey R. Hall, et al. "Pan-drug resistant Providencia rettgeri contributing to a fatal case of COVID-19." Journal of Medical Microbiology 70, no. 8 (2021). http://dx.doi.org/10.1099/jmm.0.001406.

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Following prolonged hospitalization that included broad-spectrum antibiotic exposure, a strain of Providencia rettgeri was cultured from the blood of a patient undergoing extracorporeal membrane oxygenation treatment for hypoxic respiratory failure due to COVID-19. The strain was resistant to all antimicrobials tested including the novel siderophore cephalosporin, cefiderocol. Whole genome sequencing detected ten antimicrobial resistance genes, including the metallo-β-lactamase bla NDM-1, the extended-spectrum β-lactamase bla PER-1, and the rare 16S methyltransferase rmtB2.
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17

Fan, Lixia, Jiwen Pan, Jifeng Zeng, et al. "An outbreak of Providencia rettgeri bacteremia at a Ptyas mucosus farm in Hainan, China." Frontiers in Microbiology 15 (July 11, 2024). http://dx.doi.org/10.3389/fmicb.2024.1353603.

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AimTo describe the histopathology and etiology of an outbreak of respiratory disease at a Ptyas mucosus farm in Hainan, China.Methods and resultsThe etiology was confirmed by gross examination and microscopic analysis. The bacterial isolates from blood and internal organs were identified by biochemical analysis and 16S rRNA gene sequencing. The virulence and antibiotic resistance characteristics of the isolates were further demonstrated by polymerase chain reaction (PCR), disk diffusion testing, and LD50 analysis in Kunming mice. Histopathological analysis of the diseased P. mucosus revealed s
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18

FUADI, Hanifah, Prayoga SURYADARMA, Khaswar SYAMSU, et al. "Isolation and selection of siderophore-producing bacteria from roots of Simadu pineapple (Ananas comosus) in Subang District, West Java." E-Journal Menara Perkebunan 90, no. 2 (2022). http://dx.doi.org/10.22302/iribb.jur.mp.v90i2.502.

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Abstrak Bakteri mampu menghasilkan siderofor umtuk mengkelat besi dalam lingkungan dan digunakan oleh tanaman sebagai kofaktor dalam pembentukan klorofil untuk pembentukan glukosa. Penelitian ini bertujuan untuk mendapatkan bakteri penghasil siderofor tinggi dari akar nanas Simadu, Kabupaten Subang Provinsi Jawa Barat. Bakteri diisolasi dari akar nanas Simadu, kemudian diseleksi dengan membedakan morfologinya dan diidentifikasi sebagai penghasil siderofor pada media Chrome Azurol S (CAS). Diperoleh 10 isolat bakteri (M1 sampai dengan M10) yang mampu menghasilkan siderofor. Isolat bakteri M7 me
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19

Ahsan, Ali, Sana Tanveer, Komal Shahzadi, Sidra Gull, and Basharat Ali. "Genetic and Functional Diversity of Bacterial Strains Associated with Prunus persica (L.)." Journal of Advances in Microbiology, September 17, 2022, 75–89. http://dx.doi.org/10.9734/jamb/2022/v22i1030505.

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Aims: This study aimed to evaluate the genetic and functional diversity of bacteria associated with the surfaces of Prunus persica (L.) Batsch.
 Methodology: A total of 15 peach samples from various areas of Lahore city were collected and processed according to standard microbiological protocols. The final taxonomic status of bacterial strains was confirmed by 16S rRNA gene sequencing. Bacterial strains were screened for plant growth-promoting attributes that included auxin production, phosphate, and zinc solubilization, and hydrogen cyanide (HCN) production. Antimicrobial activity of str
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Mullinax, Sarah R., Andrea M. Darby, Anjali Gupta, Patrick Chan, Brittny R. Smith, and Robert L. Unckless. "A suite of selective pressures supports the maintenance of alleles of a Drosophila immune peptide." eLife 12 (May 30, 2025). https://doi.org/10.7554/elife.90638.3.

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The innate immune system provides hosts with a crucial first line of defense against pathogens. While immune genes are often among the fastest evolving genes in the genome, in Drosophila, antimicrobial peptides (AMPs) are notable exceptions. Instead, AMPs may be under balancing selection, such that over evolutionary timescales, multiple alleles are maintained in populations. In this study, we focus on the Drosophila AMP Diptericin A, which has a segregating amino acid polymorphism associated with differential survival after infection with the Gram-negative bacteria Providencia rettgeri. Dipter
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Zhao, Weiwei, Anqi Chen, Na Yuan, et al. "The Role of High Mobility Group Box B-1 in the Prognosis of Colorectal Cancer Based on the Changes in the Intestinal Mucosal Barrier." Technology in Cancer Research & Treatment 23 (January 2024). http://dx.doi.org/10.1177/15330338231198972.

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Background: To investigate the expression of high mobility group box B-1 (HMGB-1) in patients with colorectal cancer (CRC) and its association with clinicopathological features and prognosis in colorectal carcinoma by combining bioinformatics and clinical data analysis, and to clarify the role of HMGB-1. To examine whether HMGB-1 expression is related to the damage of the intestinal mucosal barrier, and then explore the potential HMGB-1-dependent mechanisms affecting the progression of CRC. Methods: CRC datasets of GSE12945, GSE17536, and GSE17537 from the public gene chip database were screen
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22

Sun, Qiang, Yunjie Shi, Xiaoben Liang, et al. "Interaction between the intestinal flora and the severity of diversion colitis after low anterior resection of rectal cancer." Frontiers in Oncology 13 (March 14, 2023). http://dx.doi.org/10.3389/fonc.2023.1001819.

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BackgroundDiversion colitis (DC) is nonspecific inflammation of the distal intestinal mucosa following disruption of colonic continuity with colonic dysfunction. The colonscopic score is a good tool for differentiating the severity of patients with DC. At present, no studies have analyzed the pathogenesis of DC from the perspective of the diversity and and differences of intestinal flora.MethodsRetrospective study: Clinical information were collected from patients with low rectal cancer admitted to the Department of Anorectal Surgery, Changzheng Hospital, from April 2017 to April 2019. These p
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Dong, Lingzhi, Yaben Li, Caixin Yang, et al. "Species-level microbiota of ticks and fleas from Marmota himalayana in the Qinghai-Tibet Plateau." Frontiers in Microbiology 14 (June 21, 2023). http://dx.doi.org/10.3389/fmicb.2023.1188155.

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IntroductionTicks and fleas, as blood-sucking arthropods, carry and transmit various zoonotic diseases. In the natural plague foci of China, monitoring of Yersinia pestis has been continuously conducted in Marmota himalayana and other host animals, whereas other pathogens carried by vectors are rarely concerned in the Qinghai-Tibet Plateau.MethodsIn this study, we investigated the microbiota of ticks and fleas sampling from M. himalayana in the Qinghai-Tibet Plateau, China by metataxonomics combined with metagenomic methods.ResultsBy metataxonomic approach based on full-length 16S rDNA amplico
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Yang, Fengqin, Qiongshan Ma, Xiangyan Zhang, et al. "The gut bacterial composition across life stages of Sarcophaga peregrina (Diptera: Sarcophagidae) and the effects of amikacin on their development." Journal of Medical Entomology, June 21, 2024. http://dx.doi.org/10.1093/jme/tjae071.

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Abstract Insects and microorganisms, ubiquitous organisms in the natural world, have developed intricate relationships throughout their evolutionary histories. However, most studies have concentrated on specific time points or life stages, but some limited studies have investigated the dynamics of microbial diversity within insects across life stages. Here, 16S rDNA sequencing technology was used to investigate the gut bacterial community across the life stages of Sarcophaga peregrina (Robineau-Desvoidy) (Diptera: Sarcophagidae). The results revealed that the gut bacterial diversity of S. pere
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Ren, Yue, Mengfan Tao, Xiaoliang Wang, et al. "Gut microbiota and quantitative traits divergence at different altitude of long-tailed dwarf hamsters, Cricetulus longicaudatus." Frontiers in Microbiology 15 (January 24, 2025). https://doi.org/10.3389/fmicb.2024.1531629.

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To investigate the community structure and diversity of gut microflora and their function in body mass regulation, as well as the effects of various locations on gut microbiota and Cricetulus longicaudatus body mass regulation at various elevations. We examined the diversity, abundance, and community structure of the gut microbiota of long-tailed dwarf hamsters from eight regions in Shanxi province during summer using 16S rDNA sequencing technology and analyzed the relationships between these microbiota and environmental variables as well as morphological indicators. The results revealed Firmi
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