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1

Sh. H. Kamil, R. F. ALjasani, and H. I. ALShammari. "ISOLATION, IDENTEFECATION AND EFFECINCY OF PSEUDOMONAS FLUORESCENS BACTERIA TO TERMITE MICROCEROTERMIS DIVERSUS." IRAQI JOURNAL OF AGRICULTURAL SCIENCES 54, no. 6 (2023): 1583–93. http://dx.doi.org/10.36103/ijas.v54i6.1859.

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This study was conducted to isolate the bacteria Pseudomonase fluorescens from the termite, locust, and American cockroach in the Iraqi environment and to diagnose it based on morphological, biochemical, and molecular diagnosis using the polymerase chain reaction (PCR), as well as test its pathogenicity and efficacy to termites under laboratory conditions. The results of morphological , biochemical, and molecular diagnosis using polymerase chain reaction (PCR) tests showed the isolated bacterial isolates are similar to P. fluorescens .The results of efficiency of different isolates of P. fluor
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Wiedmann, Martin, Denise Weilmeier, Sean S. Dineen, Robert Ralyea, and Kathryn J. Boor. "Molecular and Phenotypic Characterization ofPseudomonas spp. Isolated from Milk." Applied and Environmental Microbiology 66, no. 5 (2000): 2085–95. http://dx.doi.org/10.1128/aem.66.5.2085-2095.2000.

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ABSTRACT Putative Pseudomonas spp. isolated predominantly from raw and processed milk were characterized by automated ribotyping and by biochemical reactions. Isolates were biochemically profiled using the Biolog system and API 20 NE and by determining the production of proteases, lipases, and lecithinases for each isolate. Isolates grouped into five coherent clusters, predominated by the species P. putida (cluster A), P. fluorescens (cluster B), P. fragi (as identified by Biolog) or P. fluorescens (as identified by API 20 NE) (cluster C), P. fragi (as identified by Biolog) or P. putida (as id
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Rai, Anuj Kumar, Laxmi Priya Sahoo, Monalisha Sahoo, Tania Seth, and Chakradhar Patra. "Induced Resistance Mechanism in Plant and Its Importance in Agriculture." International Journal of Plant & Soil Science 36, no. 5 (2024): 1–22. http://dx.doi.org/10.9734/ijpss/2024/v36i54497.

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The search for a successful and efficient natural phenomenon of induced resistance in plants was prompted by the harmful effects that chemical pesticides and their degradation products had on the environment and human health. Ray was the first to identify plant resistance to diseases in 1901. When arabidopsis plants were injected with the pathogenic bacteria Pseudomonas fluorescens, which colonises roots, induced resistance was initially observed in these plants. There are two different kinds of induced resistance: induced biochemical defense and induced structural defense. Biochemical defense
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4

Salaheddin, K., V. Valluvaparidasan, D. Ladhalakshmi, and R. Velazhahan. " Management of bacterial blight of cotton using a mixture of Pseudomonas fluorescens and Bacillus subtilis." Plant Protection Science 46, No. 2 (2010): 41–50. http://dx.doi.org/10.17221/20/2009-pps.

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The potential of antagonistic rhizobacteria in the management of bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum (Xam) was evaluated under greenhouse and field conditions. In this study, 93 bacterial isolates from the rhizosphere of cotton were screened for their efficacy in inhibiting the growth of Xam in vitro. Among them, 21 isolates were found to inhibit the in vitro growth of Xam. These isolates were identified as Pseudomonas fluorescens and Bacillus subtilis based on phenotypic characteristics, biochemical properties and using 16S-23S intergenic transcribed sp
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Ząbkiewicz, Anna, Magdalena Myga-Nowak, Katarzyna Bandurska, Justyna Paczyńska, Anna Szybecka, and Piotr Krupa. "The Application of PCR Reaction for Identification of MHB Bacteria Species." Archives of Environmental Protection 40, no. 2 (2014): 115–22. http://dx.doi.org/10.2478/aep-2014-0020.

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Abstract This study characterizes mycorrhiza helper bacteria (MHB) from selected unpolluted locations as well as subjected to industrial emissions. To determine the species of bacteria isolated from the roots of ectomycorrhizal pine and birch, a method based on the sequence analysis of a 16S rRNA gene was used. The isolated bacteria were initially characterized by available biochemical methods and phenotypic observation. On the selected bacteria representatives isolation of DNA was performed, on which the PCR reaction was carried out. In this way amplified samples were automatically sequenced
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Lee, Jung-Kul, and Huimin Zhao. "Identification and Characterization of the Flavin:NADH Reductase (PrnF) Involved in a Novel Two-Component Arylamine Oxygenase." Journal of Bacteriology 189, no. 23 (2007): 8556–63. http://dx.doi.org/10.1128/jb.01050-07.

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ABSTRACT Two-component oxygenases catalyze a wide variety of important oxidation reactions. Recently we characterized a novel arylamine N-oxygenase (PrnD), a new member of the two-component oxygenase family (J. Lee et al., J. Biol. Chem. 280:36719-36728, 2005). Although arylamine N-oxygenases are widespread in nature, aminopyrrolnitrin N-oxygenase (PrnD) represents the only biochemically and mechanistically characterized arylamine N-oxygenase to date. Here we report the use of bioinformatic and biochemical tools to identify and characterize the reductase component (PrnF) involved in the PrnD-c
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7

Al-Bayati, Majid Ahmed, Mohammed Fadhil AboKsour, Buthainah Mohammed Taha, Nadheema Hammood Hussein, and Sarah Yahya Haider. "Prevalence of <i>BlaOXA-48, BlaDIM-1</i>, and <i>BlaKPC1 </i>genes in carbapenem-resistant <i>Pseudomonas </i>species isolated from wastewater and clinical samples from Baghdad hospitals." Journal of Applied and Natural Science 17, no. 1 (2025): 24–30. https://doi.org/10.31018/jans.v17i1.6129.

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Antibiotic resistance poses a significant threat to public health, driven by misuse and overuse of antibiotics in healthcare and agriculture. Among Gram-negative bacteria, carbapenem-resistant Pseudomonas species are particularly worrisome due to their ability to harbor resistance genes such as blaOXA-48, blaDIM-1, and blaKPC1. The present study aimed to determine the prevalence of blaOXA-48, blaDIM-1, and blaKPC1 genes in Pseudomonas isolates from wastewater and clinical samples collected from two Baghdad hospitals. Sixty-nine (69) samples, including blood, urine, wounds, ear swabs, and waste
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8

Zhao, Shuai, Guoli Yang, Xiaochen Xie, et al. "Whole-Cell Display of Phosphotransferase in Escherichia coli for High-Efficiency Extracellular ATP Production." Biomolecules 12, no. 1 (2022): 139. http://dx.doi.org/10.3390/biom12010139.

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Adenosine triphosphate (ATP), as a universal energy currency, takes a central role in many biochemical reactions with potential for the synthesis of numerous high-value products. However, the high cost of ATP limits industrial ATP-dependent enzyme-catalyzed reactions. Here, we investigated the effect of cell-surface display of phosphotransferase on ATP regeneration in recombinant Escherichia coli. By N-terminal fusion of the super-folder green fluorescent protein (sfGFP), we successfully displayed the phosphotransferase of Pseudomonas brassicacearum (PAP-Pb) on the surface of E. coli cells. Th
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9

Elshafie, Hazem S., Sadeek A. Sadeek, Ippolito Camele, and Amira A. Mohamed. "Biochemical Characterization of New Gemifloxacin Schiff Base (GMFX-o-phdn) Metal Complexes and Evaluation of Their Antimicrobial Activity against Some Phyto- or Human Pathogens." International Journal of Molecular Sciences 23, no. 4 (2022): 2110. http://dx.doi.org/10.3390/ijms23042110.

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Four novel ligand-metal complexes were synthesized through the reaction of Fe(III), pleaseCo(II), Zn(II), and Zr(IV) with Schiff base gemifloxacin reacted with ortho-phenylenediamine (GMFX-o-phdn) to investigate their biological activities. Elemental analysis, FT-IR, 1H NMR, UV-visible, molar conductance, melting points, magnetic susceptibility, and thermal analyses have been carried out for insuring the chelation process. The antimicrobial activity was carried out against Monilinia fructicola, Aspergillus flavus, Penicillium italicum, Botrytis cinerea, Escherichia coli, Bacillus cereus, Pseud
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10

Balaž, J., R. Iličić, S. Maširević, D. Jošić, and S. Kojić. "First Report of Pseudomonas syringae pv. syringae Causing Bacterial Leaf Spots of Oil Pumpkin (Cucurbita pepo) in Serbia." Plant Disease 98, no. 5 (2014): 684. http://dx.doi.org/10.1094/pdis-07-13-0714-pdn.

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Oil pumpkin (Cucurbita pepo L.) is commonly used for oil production, mainly in central and eastern Europe (1). In Serbia, it grows only in the north (Vojvodina Province), up to 1,500 ha. In June 2008, typical bacterial spot symptoms (dark green, water-soaked, transparent and greasy spots with yellow margins) were observed for the first time, cultivated at the experimental fields near Backi Petrovac. Since then, bacterial spots were regularly observed on oil pumpkin in the beginning of the growing seasons and during rainy weather, with disease incidence ranging from 5 to 20%. Bacteria isolated
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11

Benlioglu, K., Ü. Özyilmaz, and D. Ertan. "First Report of Bacterial Blight Caused by Pseudomonas syringae pv. pisi on Pea in Turkey." Plant Disease 94, no. 7 (2010): 923. http://dx.doi.org/10.1094/pdis-94-7-0923a.

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In April of 2009, leaf blight symptoms were observed on field peas (Pisum sativum L.) grown in Söke, Torbali, and Ödemis counties in the Aegean Region of Turkey. Field inspections revealed disease incidence as high as 45% and the disease was found in 13 commercial fields. Initial symptoms consisted of small, dark green, water-soaked lesions on leaves, stipules, and stems near ground level. Lesions often enlarged and coalesced and turned chocolate brown with a water-soaked margin. Stem infections usually coalesced and girdled the stem spreading upward to stipules and leaflets forming a fan-like
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12

Ibrar Wali, Syeda Asma Bano, Sobia Nisa, et al. "Isolation and Identification of Bacillus Subtilis and Pseudomonas Fluorescens from Wheat Rhizosphere and Their Use as Biocontrol Agents." Indus Journal of Bioscience Research 2, no. 2 (2024): 918–31. https://doi.org/10.70749/ijbr.v2i02.295.

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Some bacteria may be used as biocontrol agents against fungal pathogens. Biocontrol agents are environment friendly and cost effective for controlling different plant pathogens. Fungal plant pathogens cause detrimental effects on plants causing diseases and yield loss. The bacterial strains Pseudomonas fluorescens and Bacillus subtilis live abundantly in rhizospheric soil and have antagonistic activity against other organisms. The objective of present study was to isolate and identify the Pseudomonas fluorescens and Bacillus subtilis from rhizospheric soil of Triticum aestivum and their use as
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13

Bouaichi, .A, Benkirane R., A.AMEUR, S.SADIK, Benbouazza A., and H. Achbani E. "Characterization of Pantoea agglomerans Strains Isolated from Olive Knot disease in Morocco." Biolife 3, no. 2 (2022): 528–34. https://doi.org/10.5281/zenodo.7270238.

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<strong>ABSTRACT</strong> On the base of prospecting conducted during the agriculture season 2012-2013, the results revealed that the olive knot disease caused by <em>Pseudomonas</em> <em>savastanoi</em> pv.<em>savastanoi</em> was observed in different olive groves in Morocco. The disease incidence varies with location and cultivar. However, high disease incidence was reported in Taounate, Ouazzane and Meknes. The biochemical and the molecular tests were revealed the presence of saprophytic bacteria; <em>Pantoea agglomerans</em>, <em>Enterobacter</em> sp., <em>Pseudomonas </em>sp., associated
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14

Rathour, T. P., P. K. Nimbolkar, L. Wangchu, A. S. Mailappa, and N. Devachandra. "Responses of Different Levels of Pruning and Biofertilizer on Fruit Growth and Quality of Assam Lemon [Citrus limon (L.) Burm] under the Foothills of Arunachal Pradesh, India." International Journal of Environment and Climate Change 13, no. 8 (2023): 1356–65. http://dx.doi.org/10.9734/ijecc/2023/v13i82081.

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The present experiment was conducted in the Department of Fruit Science, College of Horticulture and Forestry, Pasighat, Arunachal Pradesh, during the year 2021-2022. The trial was laid out using two factorial RBD replicated thrice. Assam lemon trees were pruned at 25% (P1), 50% (P2) from the top apex and soil drenching with Pseudomonas fluorescens @ 90g/plant (B1), Trichoderma @ 90g/plant (B2), Azotobacter @ 15g/plant (B3) and a combination of PGPR (Pseudomonas fluorescens) @ 90g/plant + Trichoderma @ 90g/plant + Azotobacter @ 15g/plant (B4) at two feet away from the tree trunk with the inter
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15

Ilieva, Yana, Maya Margaritova Zaharieva, Lyudmila Dimitrova, et al. "Preliminary Data on Escherichia coli, Yersinia enterocolitica, and Other Bacteria, as Well as Absent African Swine Fever Virus in the Gut Microbiota of Wild Mice and Voles from Bulgaria." Microbiology Research 14, no. 4 (2023): 1788–819. http://dx.doi.org/10.3390/microbiolres14040123.

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Small mammals are bioindicator organisms, and, through their gut microbiota (GM), could be carriers of pathogens and resistant bacteria. Also, wild GM composition has been suggested to have large implications for conservation efforts. Seventeen bacterial species were obtained from intestinal samples of Bulgarian yellow-necked mice (Apodemus flavicollis) and bank voles (Myodes glareolus) via classic microbiological cultivation and biochemical identification. Twelve Gram-negative—Escherichia coli, Yersinia enterocolitica, Yersinia kristensenii, Hafnia alvei, Serratia liquefaciens, Serratia marce
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Hsueh, Po-Ren, Lee-Jene Teng, Hui-Ju Pan, et al. "Outbreak of Pseudomonas fluorescensBacteremia among Oncology Patients." Journal of Clinical Microbiology 36, no. 10 (1998): 2914–17. http://dx.doi.org/10.1128/jcm.36.10.2914-2917.1998.

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From 7 to 24 March 1997, four patients developed Pseudomonas fluorescens bacteremia at the hospital; one on the oncology ward and the other three in the chemotherapy room. These patients all had underlying malignancies and had the Port-A-Cath (Smiths Industries Medical Systems, Deltec, Inc., St. Paul, Minn.) implants. Three patients had primary bacteremia, and one had Port-A-Cath-related infection. None of these patients had received a blood transfusion before the episodes of bacteremia. All patients recovered: two received antimicrobial agents with in vitro activity against the isolates, and
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17

Khaledi, Fahimeh, Hamidreza Balouchi, Mohsen Movahhedi Dehnavi, Amin Salehi, and Beata Dedicova. "Mitigating Drought Stress in Maize: Synergistic Effects of Zinc Sulfate and Pseudomonas spp. on Physiological and Biochemical Responses." Plants 14, no. 10 (2025): 1483. https://doi.org/10.3390/plants14101483.

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This study aimed to evaluate the synergistic effects of zinc sulfate and Pseudomonas spp. in terms of mitigating drought stress in maize (Zea mays L.) by analyzing physiological, biochemical, and morphological responses under field conditions. A two-year (2018–2019) field experiment investigated two irrigation levels (optimal and moderate stress) and twelve treatment combinations of zinc sulfate application methods (without fertilizer, soil, foliar, and seed priming) with zinc-solubilizing bacteria (no bacteria, Pseudomonas fluorescens, and Pseudomonas aeruginosa). Drought stress significantly
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Tasić, Srđan, and Aleksandar Janjić. "PSEUDOMONAS FLUORESCENS IN SHEEP MILK GREEK YOGHURT FROM VLASINA – A BIOCHEMICAL CHARACTERIZATION." KNOWLEDGE - International Journal 54, no. 3 (2022): 421–24. http://dx.doi.org/10.35120/kij5403421t.

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Pseudomonas fluorescens is an aerobic, rod-shaped, non-sporulating gram-negative bacteria, mostlyfound in soil, decaying organic matter and feces. This species contaminates the milk mainly through animal feeddust, silage, utensils and polluted water. In addition to casein digestion, this psychotropic and lipolytic speciesgenerates butyric and caproic acids by fermentation of milk fat, which release a strong unpleasant odor and give arancid and bitter taste to dairy products. In this study we tested 48 hours old sheep's sour milk, produced in ahousehold in Vlasina, southeastern Serbia, using tr
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19

E. H., Achbani, S. Sadik, R. El Kahkahi, A. Benbouazza, and H. Mazouz. "First Report on Pseudomonas marginalis Bacterium Causing Soft Rot of Onion in Morocco." Atlas Journal of Biology 3, no. 2 (2017): 218–23. http://dx.doi.org/10.5147/ajb.v3i2.33.

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Pseudomonas marginalis is an important postharvest pathogen causing soft rot in a wide variety of harvested fruits and vegetables. Isolated strains from rotten onion bulbs based on morphological characteristics, were tested for pathogenecity on tobacco. Pathogenic strains underwent a biochemical test which detected the presence of P. marginalis. Symptoms were reproduced by inoculating this species to onion leaves and bulbs. Based on ARN16S sequencing, reported associated species (Pantoea agglomerans, Pseudomonas fluorescens, Klebsiella oxytoca) were also confirmed. This study reports for the f
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20

Sharma, Harsha, Mohd Anul Haq, Ashok Kumar Koshariya, Anil Kumar, Sandeep Rout, and Karthikeyan Kaliyaperumal. "“Pseudomonas fluorescens” as an Antagonist to Control Okra Root Rotting Fungi Disease in Plants." Journal of Food Quality 2022 (April 22, 2022): 1–8. http://dx.doi.org/10.1155/2022/5608543.

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The common bacteria found in fruit and vegetables are Pseudomonas fluorescens which is Germ-negative and is rod-shaped. Pseudomonas fluorescens has been originated from the rhizosphere of Roorkee-grown okra. The presented work involves recognizing and controlling the isolates of Pseudomonas fluorescens. The scope of the proposed work is that the technique used here is a unique strategy to plant protection and control of rotting fungus diseases based on the recognition and management of Pseudomonas fluorescens isolates. Antagonist effect occurs commonly in vegetable and fruit plants. The main g
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Liao, Ching-Hsing, and Daniel E. McCallus. "Biochemical and Genetic Characterization of an Extracellular Protease from Pseudomonas fluorescensCY091." Applied and Environmental Microbiology 64, no. 3 (1998): 914–21. http://dx.doi.org/10.1128/aem.64.3.914-921.1998.

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ABSTRACT Pseudomonas fluorescens CY091 cultures produce an extracellular protease with an estimated molecular mass of 50 kDa. Production of this enzyme (designated AprX) was observed in media containing CaCl2 or SrCl2 but not in media containing ZnCl2, MgCl2, or MnCl2. The requirement of Ca2+ (or Sr2+) for enzyme production was concentration dependent, and the optimal concentration for production was determined to be 0.35 mM. Following ammonium sulfate precipitation and ion-exchange chromatography, the AprX in the culture supernatant was purified to near electrophoretic homogeneity. Over 20% o
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22

Hamza, Amany, Ahmed Mohamed, and Aly Derbalah. "Unconventional alternatives for control of tomato root rot caused by Rhizoctonia solani under greenhouse conditions." Journal of Plant Protection Research 56, no. 3 (2016): 298–305. http://dx.doi.org/10.1515/jppr-2016-0046.

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Abstract This study was done to assess the antifungal effect of some biocontrol agents effective microorganisms (EMs1), Pseudomonas fluorescens, and Bacillus pumilus, titanium dioxide (TiO2) nanoparticles, black cumin and wheat germ oils as well as the recommended fungicide (flutolanil) against root rot of tomato. Moreover, gas chromatography-mass spectrometry (GC-MS) examination was completed to identify the bioactive compounds in plant oils (dark cumin and wheat germ). Also the impact of these medicines on some biochemical and growth parameters of tomato was examined. Flutolanil was the best
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KALIESWARI, N. "Biochemical characterization of Pseudomonas fluorescens against Alterneria alternata in ashwagandha." AGRICULTURE UPDATE 12, Special-5 (2017): 1190–95. http://dx.doi.org/10.15740/has/au/12.techsear(5)2017/1190-1195.

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Mustafa Sameer Midhat and Suha Maher Abed. "Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 087–98. http://dx.doi.org/10.30574/gscbps.2023.23.1.0144.

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The bacteria Pseudomonas aeruginosa were isolated and diagnosed and compared with Pseudomonas fluorescens for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and Pseudomonas fluorescens and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitative level using the phytic technique. Carry out a sensitivi
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Sivagamasundari, U., and A. Gandhi. "Enhancement of Vegetative Parameters of Brinjal in Proplates by the Application of Bacterial Endophytes - Azospirillum brasilense and Pseudomonas fluorescens." International Letters of Natural Sciences 27 (October 2014): 14–18. http://dx.doi.org/10.18052/www.scipress.com/ilns.27.14.

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An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing abi
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Sivagamasundari, U., and A. Gandhi. "Enhancement of Vegetative Parameters of Brinjal in Proplates by the Application of Bacterial Endophytes - <i>Azospirillum brasilense</i> and<i> Pseudomonas fluorescens</i>." International Letters of Natural Sciences 27 (October 15, 2014): 14–18. http://dx.doi.org/10.56431/p-bq77ed.

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An experiment was carried out with endophytic fixing bacteria Azospirillum brasilense and Pseudomonas fluorescens isolated from brinjal, in different combinations with inorganic fertilizers by seed inoculation of brinjal to observe preliminary vegetative growth at 15th and 30th day and pigment contents in vegetable nursery bed (proplates). A total number of 28 endophytic bacteria isolated from brinjal from three localities (Annamalai University, Karaikal and Putthur). Further the isolates were subjected to various biochemical tests for their species level identification and nitrogen fixing abi
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27

Mustafa, Sameer Midhat, and Maher Abed Suha. "Isolation and identification of pathogenic species of the genus Pseudomonas and study of antibiotic resistance." GSC Biological and Pharmaceutical Sciences 23, no. 1 (2023): 087–98. https://doi.org/10.5281/zenodo.7924953.

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The bacteria&nbsp;<em>Pseudomonas aeruginosa</em>&nbsp;were isolated and diagnosed and compared with&nbsp;<em>Pseudomonas fluorescens</em>&nbsp;for the period between September 2021 and March 2022, in Ghazi Hariri Hospital, Yarmouk and the Medical City.) to investigate Pseudomonas aeruginosa and&nbsp;<em>Pseudomonas fluorescens</em>&nbsp;and other species study antibiotic resistance. (90) samples were collected using a cotton swab and a sterile container. The diagnosis was made based on the culture characteristics and biochemical tests, after which the diagnosis was confirmed at the qualitativ
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Debta, Harish, T. K. Kunhamu, A. V. Santhoshkumar, V. Jamaludheen, N. K. Binu, and K. C. Jisha. "Biochemical Changes and Germination Response of Indian Sandalwood (Santalum album L.) Seeds Subjected to Biopriming Techniques and Variable Post-priming Storage." Archives of Current Research International 24, no. 10 (2024): 321–35. http://dx.doi.org/10.9734/acri/2024/v24i10935.

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Understanding of the storage behaviour of endangered plant species such as Indian Sandalwood, Santalum album L. is important for successful conservation. We tested biopriming method with five biopriming agents (e.g. Effective microorganisms; Plant growth promoting rhizobacteria (PGPR-I and PGPR-II); Pseudomonas fluorescens and Piriformospora indica) with four uniform durations of treatments viz. 2, 4, 6 and 8 days. Hydropriming was also performed with similar condition for comparison of seed performance. The study examined the biochemical changes that occur in Sandalwood seeds during different
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Orio, A. G. Albarracín, E. Brücher, M. C. Plazas, et al. "First Report of Stewart's Wilt of Maize in Argentina Caused by Pantoea stewartii." Plant Disease 96, no. 12 (2012): 1819. http://dx.doi.org/10.1094/pdis-07-12-0668-pdn.

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Stewart's wilt is a serious disease of corn (Zea mays L.) caused by the bacterium Pantoea stewartii subsp. stewartii (Pss). Typical symptoms of infected fields and dent corn are longitudinal streaks with irregular or wavy margins, which are parallel to the veins and may extend the length of the leaf. These pale to green yellow lesions become dry and brown as the disease progresses producing a leaf blight (4). During the growing seasons 2010 to 2011 and 2011 to 2012, symptoms of bacterial leaf blight of corn were observed in central Argentina maize fields, with an incidence of 54% in Córdoba pr
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Patait, Neha N., Apet, K. T., Chapke, S. M., and Magar, S. J. "Induction of Systemic Resistance Developed by Bioagents to Protect Papaya against Papaya Ring Spot Virus." Journal of Advances in Biology & Biotechnology 28, no. 3 (2025): 217–27. https://doi.org/10.9734/jabb/2025/v28i32084.

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Papaya ring spot disease, caused by the Papaya ring spot virus (PRSV), is a major limiting factor in papaya cultivation in the Marathwada region of Maharashtra, it has potential to cause up to 100% yield loss. The present study aimed to investigate the effect of eleven bioagents viz., Trichoderma asperellum, Trichoderma harzianum, Verticillium lecanii, Bacillus subtilis, Pseudomonas fluorescens, PPMF, Streptomyces californicus, Metarhizium anisopliae, Beauveria bassiana, Biomix and an untreated control, in inducing resistance against PRSV under greenhouse conditions. The bioagents were applied
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31

Vela, Ana I., María C. Gutiérrez, Enevold Falsen, et al. "Pseudomonas simiae sp. nov., isolated from clinical specimens from monkeys (Callithrix geoffroyi)." International Journal of Systematic and Evolutionary Microbiology 56, no. 11 (2006): 2671–76. http://dx.doi.org/10.1099/ijs.0.64378-0.

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An unusual Gram-negative, catalase- and oxidase-positive, rod-shaped bacterium isolated from different clinical samples from two monkeys (Callithrix geoffroyi) was characterized by phenotypic and molecular genetic methods. The micro-organism was tentatively identified as a Pseudomonas species on the basis of the results of cellular morphological and biochemical tests. Fatty acid studies confirmed this generic placement and comparative 16S rRNA gene sequencing studies demonstrated that the unknown isolates were phylogenetically closely related to each other (100 % sequence similarity) and were
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32

Karnwal, Arun, and Aradhana Dohroo. "Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan." F1000Research 7 (January 25, 2018): 112. http://dx.doi.org/10.12688/f1000research.13644.1.

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Background: It is assumed that plant growth regulators produced by beneficial bacterial species could also influence plant growth. IAA is a major plant growth regulator responsible for stimulation of plant growth. There are several microorganisms which are naturally responsible for L- tryptophan metabolism. Methods: In total, 56 indigenous morphologically distinct isolates from rice roots were selected and subsequently characterized with biochemical tests, 16S rRNA sequencing and plant growth promoting activities. Pseudomonas fluorescens RE1 (GenBank: MF102882.1) and RE17 (GenBank: MF103672.1)
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33

Smith-Grenier, L. L., and A. Adkins. "Isolation and characterization of soil microorganisms capable of utilizing the herbicide diclofop-methyl as a sole source of carbon and energy." Canadian Journal of Microbiology 42, no. 3 (1996): 221–26. http://dx.doi.org/10.1139/m96-033.

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Six nonfermentative Gram-negative bacilli were isolated from Manitoban soils after enrichment with diclofopmethyl. Microscopic examination and physiological and biochemical tests have identified the organisms as Sphingomonas paucimobilis, Acinetobacter baumannii, Chryseomonas luteola, Pseudomonas aureofaciens, Pseudomonas cepacia, and Pseudomonas fluorescens. Growth curve studies showed that each of the isolates was able to grow in minimal medium with diclofop-methyl as the sole source of carbon and energy. Chemical analysis confirmed that all of the added diclofop-methyl (1.5 μg ∙ mL−1) had b
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34

Dynes, James J., John R. Lawrence, Darren R. Korber, George D. W. Swerhone, Gary G. Leppard, and Adam P. Hitchcock. "Morphological and biochemical changes inPseudomonas fluorescensbiofilms induced by sub-inhibitory exposure to antimicrobial agents." Canadian Journal of Microbiology 55, no. 2 (2009): 163–78. http://dx.doi.org/10.1139/w08-109.

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Confocal laser scanning microscopy (CLSM) and scanning transmission X-ray microscopy (STXM) were used to examine the morphological and biochemical changes in Pseudomonas fluorescens biofilms grown in the presence of subinhibitory concentrations of 4 antimicrobial agents: triclosan, benzalkonium chloride, chlorhexidine dihydrochloride, and trisodium phosphate. CLSM analyses using the stains SYTO9 and propidium iodide indicated that the antimicrobial agents affected cell membrane integrity and cellular density to differing degrees. However, fluorescein diacetate assays and plate counts demonstra
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35

Sukdolak, S., S. Solujic-Sukdolak, and Lj Krstic. "Microbiological transformation of a synthetic benzofurane type lactone." Journal of the Serbian Chemical Society 65, no. 2 (2000): 103–11. http://dx.doi.org/10.2298/jsc0002103s.

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The aim of this work was the investigation of the biotransformation ability of a synthetic benzofurane type g-lactone. This work was based on the microbiological reactions of Aspergillus niger, Saccharomyces cerevisiae, Bacillus mycoides, Agrobacterium tumefaciens, Pseudomonas glicinea and Pseudomonas fluorescens to obtain new active derivatives. Four biotransformation products were determined. The lactone was used for two purposes, as an additive for the nutrient substrate and as a source of organic carbon.
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36

Adham, Nehad Z., Abeer Abd El-Hady та Nadia Naim. "Biochemical studies on the microbial Δ1-dehydrogenation of cortisol by Pseudomonas fluorescens". Process Biochemistry 38, № 6 (2003): 897–902. http://dx.doi.org/10.1016/s0032-9592(02)00098-5.

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37

Barelmann, Insa, Kambiz Taraz, Herbert Budzikiewicz, Valérie Geoffroy, and Jean-Marie Meyer. "The Structures of the Pyoverdins from Two Pseudomonas fluorescens Strains Accepted Mutually by Their Respective Producers." Zeitschrift für Naturforschung C 57, no. 1-2 (2002): 9–16. http://dx.doi.org/10.1515/znc-2002-1-202.

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From Pseudomonas fluorescens PL7 and PL8 structurally related pyoverdins were isolated and their primary structures were elucidated by spectroscopic methods and degradation reactions. Despite of some structural differences both Fe(III) complexes are taken up by either strain with a high rate. The implications regarding the recognition at the cell surface are discussed.
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38

Kirner, Sabine, Philip E. Hammer, D. Steven Hill, et al. "Functions Encoded by Pyrrolnitrin Biosynthetic Genes from Pseudomonas fluorescens." Journal of Bacteriology 180, no. 7 (1998): 1939–43. http://dx.doi.org/10.1128/jb.180.7.1939-1943.1998.

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ABSTRACT Pyrrolnitrin is a secondary metabolite derived from tryptophan and has strong antifungal activity. Recently we described four genes,prnABCD, from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin. In the work presented here, we describe the function of each prn gene product. The four genes encode proteins identical in size and serology to proteins present in wild-type Pseudomonas fluorescens, but absent from a mutant from which the entire prn gene region had been deleted. The prnA gene product catalyzes the chlorination of l-tryptophan to form 7-chloro-l-tryptophan.
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39

LIMA, Ana Beatriz Mori, Lara Stefânia Netto de Oliveira LEÃO-VASCONCELOS, Dayane de Melo COSTA, et al. "Pseudomonas spp. ISOLATED FROM THE ORAL CAVITY OF HEALTHCARE WORKERS FROM AN ONCOLOGY HOSPITAL IN MIDWESTERN BRAZIL." Revista do Instituto de Medicina Tropical de São Paulo 57, no. 6 (2015): 513–14. http://dx.doi.org/10.1590/s0036-46652015000600009.

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This cross-sectional study, performed in an oncology hospital in Goiania, aimed to characterize the prevalence of oral colonization and antimicrobial susceptibility of Pseudomonas spp. isolated from the saliva of healthcare workers. Microorganisms were subjected to biochemical tests, susceptibility profile, and phenotypic detection. Of 76 participants colonized with Gram negative bacilli, 12 (15.8%) harbored Pseudomonas spp. Of all isolates, P. aeruginosa (75.0%), P. stutzeri (16.7%), and P. fluorescens (8.3%), were resistant to cefoxitin, and therefore likely to be AmpC producers. The results
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ADAN GÖKBULUT, Aysun, and Alper ARSLANOĞLU. "Purification and biochemical characterization of an extracellular lipase from psychrotolerant Pseudomonas fluorescens KE38." TURKISH JOURNAL OF BIOLOGY 37 (2013): 538–46. http://dx.doi.org/10.3906/biy-1211-10.

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Singh, Saurabh Kumar, P. N. Singh, P. C. Shrivastava, D. C. Dimri, S. Tripathi, and Alakh Narayan. "Influence of Pseudomonas fluorescens and ferrous sulphate on biochemical parameters of peach leaves." Journal of Applied Horticulture 21, no. 01 (2019): 77–80. http://dx.doi.org/10.37855/jah.2019.v21i01.14.

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42

Koche, Mina, RM Gade, RB Kothikar, and Aparna Tekade. "Biochemical studies on genotypic characterization of Pseudomonas fluorescens isolates by PCR-RAPD analysis." International Journal of Chemical Studies 8, no. 2 (2020): 2915–17. http://dx.doi.org/10.22271/chemi.2020.v8.i2as.9191.

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43

NAGAI, Katsuhiro, Shin OHTA, Hiroshi ZENDA, Hideki MATSUMOTO, and Masahiro MAKINO. "Biochemical Characterization of a Pseudomonas fluorescens Strain Isolated from a Benzalkonium Chloride Solution." Biological & Pharmaceutical Bulletin 19, no. 6 (1996): 873–75. http://dx.doi.org/10.1248/bpb.19.873.

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44

Chakraborty, Kajal, and R. Paulraj. "Purification and Biochemical Characterization of an Extracellular Lipase from Pseudomonas fluorescens MTCC 2421." Journal of Agricultural and Food Chemistry 57, no. 9 (2009): 3859–66. http://dx.doi.org/10.1021/jf803797m.

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45

de Souza, Jorge T., Marjan de Boer, Pieter de Waard, Teris A. van Beek, and Jos M. Raaijmakers. "Biochemical, Genetic, and Zoosporicidal Properties of Cyclic Lipopeptide Surfactants Produced by Pseudomonas fluorescens." Applied and Environmental Microbiology 69, no. 12 (2003): 7161–72. http://dx.doi.org/10.1128/aem.69.12.7161-7172.2003.

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ABSTRACT Zoospores play an important role in the infection of plant and animal hosts by oomycetes and other zoosporic fungi. In this study, six fluorescent Pseudomonas isolates with zoosporicidal activities were obtained from the wheat rhizosphere. Zoospores of multiple oomycetes, including Pythium species, Albugo candida, and Phytophthora infestans, were rendered immotile within 30 s of exposure to cell suspensions or cell culture supernatants of the six isolates, and subsequent lysis occurred within 60 s. The representative strain SS101, identified as Pseudomonas fluorescens biovar II, reduc
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46

Boulkour Touioui, Souraya, Nadia Zaraî Jaouadi, Khelifa Bouacem, et al. "Biochemical and molecular characterization of a novel metalloprotease from Pseudomonas fluorescens strain TBS09." International Journal of Biological Macromolecules 107 (February 2018): 2351–63. http://dx.doi.org/10.1016/j.ijbiomac.2017.10.116.

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47

Theng, A. D., R. M. Gade, P. R. Padghan, and Priya C. Atram. "BIOCHEMICAL AND MORPHOLOGICAL CHARACTERIZATION OF PSEUDOMONAS FLUORESCENS ISOLATES AND ITS EFFICACY AGAINST DAMPING-OFF IN CHILLI." Journal of Plant Disease Sciences 19, no. 2 (2024): 128–33. https://doi.org/10.48165/jpds.2024.19.02.07.

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This study examines three isolates of Pseudomonas fluorescens (PDKV Pf1, Pf2, Pf3) obtained from the chilli rhizosphere, focusing on their biochemical and morphological characteristics and their efficacy in suppressing Pythium aphanidermatum, the causal agent of damping-off in chilli. The isolates were evaluated for their ability to produce hydrogen cyanide (HCN) and indole-3-acetic acid (IAA), essential plant growth-promoting compounds. Morphologically, all isolates were identified as gram-negative and rod-shaped. Among them, PDKV Pf1 displayed the strongest biochemical activity, testing posi
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VAIDYA, KEDAR, FAHMIN AHMED, and SASISANKER PADMANABHAN. "ENHANCEMENT OF PHENOLIC CONTENT AND ANTIOXIDANT PROPERTIES THROUGH CHEMICAL AND BIOCHEMICAL REATMENT OF LIGNIN-RICH RICE STRAW RESIDUE." Cellulose Chemistry and Technology 58, no. 5-6 (2024): 529–39. http://dx.doi.org/10.35812/cellulosechemtechnol.2024.58.49.

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Valorization of lignin in biorefineries is important as this can lead to enhancement of chemical and physical properties of lignin, making it suitable for applications in polymers, composites and adhesives. Lignin valorization requires overcoming recalcitrance and tailoring for different uses. Here, lignin was extracted from rice straw lignin-rich residue at pH 12, using 4% magnesium oxide and 6% sodium hydroxide on a dry w/w basis. The extracted lignin-rich stream, which had a pH in the range of 9-10, was used further for biochemical transformation. Towards that end, Pseudomonas putida, Sphin
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49

Shandookh, Fatima Kareem, Melad Khalaf Mohammed, and Ahmed Darweesh Jabbar. "Bioremediation of hydrocarbon pollutants by Pseudomonas putida under optimal conditions." Advancements in Life Sciences 11, no. 4 (2024): 761. http://dx.doi.org/10.62940/als.v11i4.1963.

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Background: The extreme toxicity of petroleum products to human and environmental health, particularly when linked to large-scale unintentional spills, has made them one of the most serious and current environmental pollution issue. Petroleum products are a complex mixture of hydrocarbons.Methods: Oil-contaminated soil samples were gathered and utilized to isolate hydrocarbon-degrading bacterial isolates. The bacterial isolates possessing bioremediation capabilities were identified using phenotypic determination and biochemical properties. The Optimal conditions including incubation period, te
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50

Suresh, Perumal, Murukesan Rekha, Subramanian Gomathinayagam, et al. "Characterization and Assessment of 2, 4-Diacetylphloroglucinol (DAPG)-Producing Pseudomonas fluorescens VSMKU3054 for the Management of Tomato Bacterial Wilt Caused by Ralstonia solanacearum." Microorganisms 10, no. 8 (2022): 1508. http://dx.doi.org/10.3390/microorganisms10081508.

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Microbial bio-products are becoming an appealing and viable alternative to chemical pesticides for effective management of crop diseases. These bio-products are known to have potential to minimize agrochemical applications without losing crop yield and also restore soil fertility and productivity. In this study, the inhibitory efficacy of 2,4-diacetylphloroglucinol (DAPG) produced by Pseudomonas fluorescens VSMKU3054 against Ralstonia solanacearum was assessed. Biochemical and functional characterization study revealed that P. fluorescens produced hydrogen cyanide (HCN), siderophore, indole ac
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