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Artykuły w czasopismach na temat „Rapd analysis of E. aerogenes”

Autor: Grafiati

Data publikacji: 3 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Abdul Rahim, Muhammad Khairulanwar, Nur Mas Ayu Jamaludin, Jacinta Santhanam, Azrul Azlan Hamzah, and Muhamad Ramdzan Buyong. "Rapid ESKAPE Pathogens Detection Method using Tapered Dielectrophoresis Electrodes via Crossover Frequency Analysis." Sains Malaysiana 49, no. 12 (2020): 2913–25. http://dx.doi.org/10.17576/jsm-2020-4912-04.

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This paper introduces the versatile of an electrokinetic technique by using the non-uniform electric field for dielectrophoresis (DEP) application. This technique is defined as electromicrofluidics. The potential application for portable and real time detection method of Enterococcus faecium (EF), Staphylococcus aureus (SA), Klebsiella pneumoniae (KP), Acinetobacter baumannii (AB), Pseudomonas aeruginosa (PA) and Enterobacter aerogenes (EA), which are the (ESKAPE) bacteria. The MATLAB analytical modelling was used in simulating the polarisation factor and velocities of bacteria based on Clausi

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Tićac, Mateo, Tanja Grubić Kezele, Maja Abram, and Marina Bubonja-Šonje. "Evaluation of the Microbiological Performance and Potential Clinical Impact of New Rapid Molecular Assays for the Diagnosis of Bloodstream Infections." Microorganisms 13, no. 3 (2025): 616. https://doi.org/10.3390/microorganisms13030616.

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Bloodstream infection (BSI) is a critical medical emergency associated with a high mortality rate. Rapid and accurate identification of the causative pathogen and the results of antimicrobial susceptibility testing are crucial for initiating appropriate antimicrobial therapy. The aim of this study was to evaluate the performance of a new rapid PCR Molecular Mouse System (MMS) for the identification of Gram-negative bacteria (GNB) and GNB resistance genes directly from a positive blood culture (BC). The validation of these rapid multiplex assays was carried out in a real hospital setting. A tot

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Ibraheim, Hanaa, Khadeeja Madhi, Alyaa Jasim, and Hasanain Gharban. "<b>Molecular identification of <i>Klebsiella</i> species from pneumonic goats, Iraq</b>." Open Veterinary Journal 14, no. 11 (2024): 2980. https://doi.org/10.5455/ovj.2024.v14.i11.26.

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<b>Background:</b> In goats, acute and chronic respiratory infections are often characterized by a rapid progressing clinical course with a little opportunity to develop an effective antibiotic therapy. <b>Aim:</b> This study aimed to identify Klebsiella spp. in pneumonic goats, assess its antibiotic susceptibility, and confirm the molecular phylogenetics of Klebsiella spp. <b>Methods:</b> A total of 80 pneumonic goats were selected from the slaughterhouses located in Basra province (Iraq) during June to November 2023, and each animal was subjected to obtain

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Patel, Vipul R., and Nikhil Bhatt. "Isolation, development and identification of salt-tolerant bacterial consortium from crude-oil-contaminated soil for degradation of di-azo dye Reactive Blue 220." Water Science and Technology 72, no. 2 (2015): 311–21. http://dx.doi.org/10.2166/wst.2015.208.

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The objective of this study was development and characterization of a halophilic bacterial consortium for rapid decolorization and degradation of a wide range of dyes and their mixtures. The 16S rRNA gene analysis of developed halophilic consortium VN.1 showed that the bacterial consortium contained six bacterial strains, which were identified as Pseudomonas fluorescens HM480360, Enterobacter aerogenes HM480361, Shewanella sp. HM589853, Arthrobacter nicotianae HM480363, Bacillus beijingensis HM480362 and Pseudomonas aeruginosa JQ659549. Halophilic consortium VN.1 was able to decolorize up to 2

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Ngaffo, Carine M. N., Simplice B. Tankeo, Michel-Gael F. Guefack, et al. "Phytochemical analysis and antibiotic-modulating activity of Cocos nucifera, Glycine max and Musa sapientum methanol extracts against multidrug resistant Gram-negative bacteria." Investigational Medicinal Chemistry and Pharmacology 4, no. 2 (2021): 1–12. http://dx.doi.org/10.31183/imcp.2020.00053.

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Background: The rapid emergence of multidrug resistant (MDR) bacteria is occurring worldwide, endangering the efficacy of antibiotics, which have transformed medicine and saved millions of lives. Antibiotic-resistant infections are already widespread in the Sub-Saharan Africa and across the globe. To extend the search for new and more efficient antimicrobial drugs from natural sources, this work has been carried out to study the phytochemical composition and the antibacterial activities of some Cameroonian dietary plants (Cocos nucifera, Glycine max and Musa sapientum) against several MDR Gram

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HUANG, FANG, SHUANG LI, LAN LOU, JUNJUN MO, and HAO XU. "Comparative Genomic Analysis and Phenotypic Characterization of Bronchoscope-Associated Klebsiella aerogenes." Polish Journal of Microbiology 70, no. 3 (2021): 409–12. http://dx.doi.org/10.33073/pjm-2021-038.

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Bronchoscopes have been linked to outbreaks of nosocomial infections. The phenotypic and genomic profiles of bronchoscope-associated Klebsiella aerogenes isolates are largely unknown. In this work, a total of 358 isolates and 13 isolates were recovered from samples after clinical procedures and samples after decontamination procedures, respectively, over the five months. Antimicrobial susceptibility testing found seven K. aerogenes isolates exhibiting a low-level resistance to antimicrobial agents. Among seven K. aerogenes isolates, we found five sequence types (STs) clustered into three main

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Choi, Sunghee, Seulgi Kim, Seojin Woo, and Dongju Lee. "Comparative Study of Reduced Graphene Oxide Aerogels and Films for Supercapacitor Electrodes." Journal of Powder Materials 32, no. 1 (2025): 23–29. https://doi.org/10.4150/jpm.2024.00472.

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Supercapacitors, renowned for their high power density and rapid charge-discharge rates, are limited by their low energy density. This limitation has prompted the need for advanced electrode materials. The present study investigated reduced graphene oxide (rGO) in two distinct structures, as a film and as an aerogel, for use as supercapacitor electrodes. The rGO film, prepared by vacuum filtration and thermal reduction, exhibited a compact, lamellar structure, while the aerogel, synthesized through hydrothermal treatment, was a highly porous three-dimensional network. Electrochemical analyses

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Gawel, Nick J., Rory Mellinger, Eric Stout, and R. Sauve. "RAPD Analysis of Acer." HortScience 30, no. 4 (1995): 813F—813. http://dx.doi.org/10.21273/hortsci.30.4.813f.

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DNA from 27 Acer species was used for RAPD analysis. A relatively high number of phylogenically informative polymorphisms were detected, as would be expected in intraspecific comparisons. Principle coordinates analysis was used to discern groupings among the species and a RAPD-based phylogeny was constructed. As expected when making comparisons among species, very high levels of polymorphism were found. Cultivars that grouped together in the principle components analysis also grouped together in the phylogenic analysis. Parts of the phylogenic analysis do not agree with morphology-based phylog

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Tran, Que-Tien, Myrielle Dupont, Jean-Philippe Lavigne та ін. "Occurrence of Efflux Mechanism and Cephalosporinase Variant in a Population of Enterobacter aerogenes and Klebsiella pneumoniae Isolates Producing Extended-Spectrum β-Lactamases". Antimicrobial Agents and Chemotherapy 53, № 4 (2009): 1652–56. http://dx.doi.org/10.1128/aac.00822-08.

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ABSTRACT We investigated the occurrence of multidrug resistance in 44 Enterobacter aerogenes and Klebsiella pneumoniae clinical isolates. Efflux was involved in resistance in E. aerogenes isolates more frequently than in K. pneumoniae isolates (100 versus 38% of isolates) and was associated with the expression of phenylalanine arginine β-naphthylamide-susceptible active efflux. AcrA-TolC overproduction in E. aerogenes isolates was noted. An analysis of four E. aerogenes isolates for which cefepime MICs were high revealed no modification in porin expression but a new specific mutation in the Am

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Wu, Y. J., Y. Chen, J. Wang, C. X. Zhu, and B. L. Xu. "RAPD analysis of jasmine rice-specific genomic structure." Genome 49, no. 6 (2006): 716–19. http://dx.doi.org/10.1139/g06-018.

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Total genomic DNA was extracted from 29 samples of rice seed, including jasmine rice Oryza sativa L. subsp indica 'KDML105', 'KDML105'-derived varieties, nonaromatic Thailand rice, and japonica rice. Polymorphism in RAPD profiles was analyzed to explore the genomic structure specific to jasmine rice. The degree of band sharing was used to evaluate genetic distance between varieties and to construct a phylogenetic tree. RD15, CNTLR85033, and CNT87040 were found to be closest to 'KDML105', which was consistent with the true relation among them. Four RAPD fragments that cooperatively distinguishe

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Kehrenberg, Corinna, and Stefan Schwarz. "Molecular Analysis of Tetracycline Resistance inPasteurella aerogenes." Antimicrobial Agents and Chemotherapy 45, no. 10 (2001): 2885–90. http://dx.doi.org/10.1128/aac.45.10.2885-2890.2001.

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ABSTRACT Tetracycline-resistant Pasteurella aerogenes isolates obtained from the intestinal tract of swine were investigated for theirtet genes by PCR analysis and hybridization experiments. In contrast to Pasteurella isolates from the respiratory tract, tet(H) genes were detected in the chromosomal DNA of only 2 of the 24 isolates, one of which also carried two copies of atet(B) gene. All other P. aerogenes isolates carried tet(B) genes, which are the predominanttet genes among Enterobacteriaceae. A single isolate harbored a tet(B) gene as part of a truncated Tn10 element on the 4.8-kb plasmi

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12

Salem Alsaffar, Raed. "Analysis of the Genetic Distance of Several Generations of Barley (Hordeum valulgare L) by RAPD-PCR Technique." Bionatura 8, no. 1 (2023): 1–4. http://dx.doi.org/10.21931/rb/2023.08.01.23.

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Random-amplified-polymorphic-DNA(RAPD) was assayed to detect the genetic variation of 6 barley generations from Iraq. Four primers generated a total of 17 scoreable bands in RAPD analysis) and resolving power, the three polymorphic primers differed (Rp). The use of RAPD marker systems to detect the genetic distance among barley generation was discovered to be beneficial. The RAPD dendrograms indicate a diverse grouping of 6 barely specimens, although we did see that certain groups were identical in several cases. As a result, the RAPD molecular markers reveal two genetic groups in the few spec

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Scott, Michelle Pellissier, Kenneth M. Haymes, and Scott M. Williams. "Parentage analysis using RAPD PCR." Nucleic Acids Research 20, no. 20 (1992): 5493. http://dx.doi.org/10.1093/nar/20.20.5493.

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Rasmussen, H. N., J. E. Olsen, and O. F. Rasmussen. "RAPD analysis of Yersinia enterocolitica." Letters in Applied Microbiology 19, no. 5 (1994): 359–62. http://dx.doi.org/10.1111/j.1472-765x.1994.tb00475.x.

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Miyata, M., T. Aoki, V. Inglis, T. Yoshida, and M. Endo. "RAPD analysis ofAeromonas salmonicidaandAeromonas hydrophila." Journal of Applied Bacteriology 79, no. 2 (1995): 181–85. http://dx.doi.org/10.1111/j.1365-2672.1995.tb00933.x.

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Säull, T., C. Lind-Halldén, and C. Halldén. "Primer Mixtures in RAPD Analysis." Hereditas 132, no. 3 (2004): 203–8. http://dx.doi.org/10.1111/j.1601-5223.2000.00203.x.

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Tigano, Myrian S., and Salah Aljanabi. "RAPD Analysis of Nomuraea rileyi." Journal of Invertebrate Pathology 75, no. 3 (2000): 240–42. http://dx.doi.org/10.1006/jipa.1999.4920.

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Crockett, Phillip A., Prem L. Bhalla, C. K. Lee, and Mohan B. Singh. "RAPD analysis of seed purity in a commercial hybrid cabbage (Brassica oleracea var. capitata) cultivar." Genome 43, no. 2 (2000): 317–21. http://dx.doi.org/10.1139/g99-034.

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The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F1-hybrid and its parental lines was subjected to RAPD screening with 36 random decamer arbitrary primers. A total of 241 scorable products were observed with 54 (22%) being polymorphic. The RAPD data showed that the parental lines of this commercial cabbage cultivar were not very closely related. Two primers were

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Haase, A., A. Melder, H. Smith-Vaughan, D. Kemp, and B. Currie. "RAPD analysis of isolates ofBurkholderia pseudomalleifrom patients with recurrent melioidosis." Epidemiology and Infection 115, no. 1 (1995): 115–21. http://dx.doi.org/10.1017/s0950268800058179.

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SummaryTwenty-seven isolates ofBurholderia pseudomallei(formerlyPseudomonas pseudomallei) from ten patients with recurrent melioidosis were analysed by RAPD. In two cases RAPD patterns in recurrent isolates differed from the original isolates; one was considered a likely reinfection while the other may represent relapse from one of two strains initially infecting the patient. In two cases where a change in antibiotic resistance had occurred between original and relapse isolates, slight changes in RAPD patterns were found with one of the four primers used. In the other six cases the relapse was

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Warner, Jennifer M., and James D. Oliver. "Randomly Amplified Polymorphic DNA Analysis of Starved and Viable but Nonculturable Vibrio vulnificusCells." Applied and Environmental Microbiology 64, no. 8 (1998): 3025–28. http://dx.doi.org/10.1128/aem.64.8.3025-3028.1998.

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ABSTRACT Vibrio vulnificus is an estuarine bacterium capable of causing a rapidly fatal infection in humans. Because of the low nutrient levels and temperature fluctuations found in the organism’s natural habitat, the starvation state and viable but nonculturable (VBNC) state are of particular interest. A randomly amplified polymorphic DNA (RAPD) PCR protocol was developed previously for the detection of V. vulnificus strains grown in rich media and has been applied to starved and VBNC cells of V. vulnificusin the present study. As cells were subjected to starvation in artificial seawater, cha

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Lu, J., Z. Liu, and Y. Zheng. "Estimation of Genetic Variation in Grapes Based on RAPD and Isozyme Analysis." HortScience 32, no. 3 (1997): 513E—513. http://dx.doi.org/10.21273/hortsci.32.3.513e.

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Genetic relationships among 42 grape accession of at least 15 species were estimated and compared using RAPD and isozyme techniques. These accessions were either hybrids or wild collections of the Asiatic species, the American species, the European grape (V. vinifera), and muscadine grape (V. rotundifolia). A total of 196 RAPD fragments were generated from twenty 10-mer primers. The pairwise similarities among the accession ranged from 0.46 to 0.94. A dendrogram was generated based on the RAPD similarity coefficients. Species/accessions were basically grouped together in accordance with their

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Grant, Mike L., Diana M. Miller, and Alastair Culham. "Genetic and Morphological Analysis of the Origin and Identity of Perennial Lavatera (Malvaceae) Cultivars." Journal of the American Society for Horticultural Science 126, no. 5 (2001): 593–98. http://dx.doi.org/10.21273/jashs.126.5.593.

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Knowledge of the origin of Lavatera L. (tree mallows) cultivars helps to predict their cultural requirements. Eighteen accessions representing 15 cultivars, 14 accessions of 7 species, and 5 accessions of an F1 hybrid between the putative parents of the cultivars were sampled for morphological variation and for randomly amplified polymorphic DNA (RAPD) fingerprint variation. Species-specific molecular markers were identified from the RAPD profiles. Chimeral elements were not distinguishable by RAPD analysis. Principal component analysis identified the majority of the cultivars to be selections

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G. Baldaniya, Vipul, Ajay V. Narwade, Pathik B. Patel, and Nilima Karmakar. "Molecular diversity analysis of rice (Oryza sativa L.) genotypes using RAPD and SSR marker." emergent Life Sciences Research 08, no. 02 (2022): 113–23. http://dx.doi.org/10.31783/elsr.2022.82113123.

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Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeat (SSR) molecular markers were used for detecting the genetic variability of 20 rice genotypes using 79 SSRs and 30 RAPD primers. Among primers used, a set of 16 SSR and 14 RAPD markers showed polymorphism, and banding patterns were scored as 1 (present) or 0 (absent) in the datasheet which was further analyzed by using Jaccard’s similarity coefficient and SAHN clustering. The number of alleles, PIC value, and heterozygosity for individual 16 SSR and 14 RAPD markers were used to assess the degree of polymorphism among the rice ge

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Kuddus, Ruhul H., Nayema N. Kuddus, and Igor Dvorchik. "DNA polymorphism in the living fossil Ginkgo biloba from the Eastern United States." Genome 45, no. 1 (2002): 8–12. http://dx.doi.org/10.1139/g01-115.

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Random amplified polymorphic DNA (RAPD) analysis is a valuable tool in studying inter- and intra-specific genetic variations, patterns of gene expression, and for the identification of specific genes using nearly isogenic variants. Here we used RAPD analysis to study the genetic variation in Ginkgo biloba grown in the eastern United States. Our results support the evidence that Southern blot hybridization of RAPD using probes made from cloned DNA fragments allows a more accurate analysis of the RAPD pattern than dye-stained gels or Southern blot hybridization of RAPD blots using probes made fr

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Raddová, J., M. Beránek, I. Oukropec, and M. Vachůn. "RAPD Analysis of peaches within Czech National collection." Czech Journal of Genetics and Plant Breeding 39, No. 4 (2011): 113–19. http://dx.doi.org/10.17221/3728-cjgpb.

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The Random Amplified Polymorphic DNA (RAPD) technique was used to study the genetic diversity and relationships within the collection of the Czech National Plant Genetic Resources (PGR) of peaches (Prunus persica L.). The aim of the work was to elaborate a dendrogram of genetic similarity and to divide collection into clusters. 46 primers were applied to 6 cultivars differing in the place of origin, the fruit shape, the fruit colour, and in some other morphological characteristics. 12 primers were chosen which gave polymorphic repeatable strong and middle strong bands. They were subsequently u

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Sevindik, Emre, Mehmet Yavuz Paksoy, Feyzanur Topseçer, and Melike Aydoğan. "Phylogenetic Analysis of Chrysochamela (Fenzl) Boiss. (Brassicaceae) Taxa Growing in Turkey." Turkish Journal of Agriculture - Food Science and Technology 7, no. 12 (2019): 2129–32. http://dx.doi.org/10.24925/turjaf.v7i12.2129-2132.2839.

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In this study, we performed a genetic diversity analysis using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter simple sequence repeat) markers for some Chrysochamela species growing in Turkey. PCR was performed using RAPD and ISSR primers and genomic DNA samples from each specimen, and the resulting bands were scored. Phylogenetic relationships and genetic distances between the studied taxa were calculated using the PAUP analysis program. According to the RAPD analysis, the closest genetic distance was found to be 0.20000 between C. noeana populations, while the most distant values wer

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Soto, S. M., B. Guerra, M. A. González-Hevia, and M. C. Mendoza. "Potential of Three-Way Randomly Amplified Polymorphic DNA Analysis as a Typing Method for TwelveSalmonella Serotypes." Applied and Environmental Microbiology 65, no. 11 (1999): 4830–36. http://dx.doi.org/10.1128/aem.65.11.4830-4836.1999.

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ABSTRACT The potential of a three-way randomly amplified polymorphic DNA (RAPD) procedure (RAPD typing) for typing Salmonella enterica strains assigned to 12 serotypes was analyzed. The series of organisms used included 235 strains (326 isolates) collected mainly from clinical samples in the Principality of Asturias and 9 reference strains. RAPD typing was performed directly with broth cultures of bacteria by using three selected primers and optimized PCR conditions. The profiles obtained with the three primers were used to define RAPD types and to evaluate the procedure as a typing method at

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Novy, R. G., N. Vorsa, and K. Patten. "Identifying Genotypic Heterogeneity in `McFarlin' Cranberry: A Randomly-amplified Polymorphic DNA (RAPD) and Phenotypic Analysis." Journal of the American Society for Horticultural Science 121, no. 2 (1996): 210–15. http://dx.doi.org/10.21273/jashs.121.2.210.

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Highly variable productivity among Vaccinium macrocarpon (Ait.) Pursh `McFarlin' bogs in Washington has been noted by growers. The fruiting habits of 12 Washington `McFarlin' bogs, ranging from 5.7-28.4 t/ha productivity were characterized. Uprights from each bog were characterized using RAPD markers, and then used in a greenhouse pollination experiment to determine if variation in fruiting and fertility phenotypes could be associated with RAPD profiles. Fifteen RAPD profiles were identified, and genetic heterogeneity was high among the 12 bogs. An association between RAPD profiles and reprodu

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Stafne, Eric T., John R. Clark, and Kim S. Lewers. "MOLECULAR MARKER-DERIVED GENETIC SIMILARITY ANALYSIS OF A SEGREGATING BLACKBERRY POPULATION." HortScience 40, no. 3 (2005): 874b—874. http://dx.doi.org/10.21273/hortsci.40.3.874b.

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A tetraploid blackberry population that segregates for two important morphological traits, thornlessness and primocane fruiting, was tested with molecular marker analysis. Both randomly amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers were used to screen a population of 98 genotypes within the population plus the two parents, `Arapaho' and `Prime-Jim' (APF-12). RAPD analysis averaged 3.4 markers per primer, whereas SSR analysis yielded 3.0 markers per primer pair. Similarity coefficient derived from the Dice index averaged over all individuals was 63% for RAPD markers,

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Novy, Richard, Nicholi Vorsa, and Kim Patten. "011 RAPD ANALYSIS IDENTIFIES GENETIC VARIATION AND RELATEDNESS WITHIN THE CRANBERRY VARIETY MCFARLIN." HortScience 29, no. 5 (1994): 429b—429. http://dx.doi.org/10.21273/hortsci.29.5.429b.

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The cranberry cultivar `HcFarlin', selected from a natural bog in Massachusetts in 1874, has become the most widely grown cultivar in the Northwestern U.S.A. Washington state growers have noted variable productivity among `McFarlin' bogs. The determination of whether there is a genetic basis for the variability has been made difficult by a paucity of reliable morphological descriptors in cranberry. A random amplified polymorphic DNA (RAPD) analysis of 45 clones sampled from 12 WA `McFarlin' bogs identified 17 unique RAPD profiles. Cluster analysis identified 7 groups having various numbers of

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Adarsh, Anupam, Pankaj Kumar, Bishun D. Prasa, Randhir Kumar, and Hemant K. Singh. "Genetic Diversity Analysis of Bitter Gourd (Momordica charantia L.) Germplasm using Molecular Markers." Indian Journal of Plant Genetic Resources 36, no. 03 (2023): 442–48. http://dx.doi.org/10.61949/0976-1926.2023.v36i03.14.

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Bitter gourd [Momordica charantia L.] is an important cucurbitaceous crop of medicinal and nutraceutical value. The present study was conducted to compare the efficacy of random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) molecular markers for genetic diversity analysis of bitter gourd. Twenty-one genotypes of bitter gourd, collected from different parts of India, were used for diversity analysis using two different types of markers. RAPD and ISSR markers yielded an average of 7.16 and 5.73 amplicon per primer, respectively. In the present study, RAPD and ISSR sho

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Kazan, Kemal, John M. Manners, and Don F. Cameron. "Inheritance of random amplified polymorphic DNA markers in an interspecific cross in the genus Stylosanthes." Genome 36, no. 1 (1993): 50–56. http://dx.doi.org/10.1139/g93-007.

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The inheritance of random amplified polymorphic DNA (RAPD) markers generated via the polymerase chain reaction amplification of genomic DNA sequences in an F2 family of an interspecific cross between Stylosanthes hamata and S. scabra was investigated. An initial comparison between the parental species, S. hamata cv. Verano and S. scabra cv. Fitzroy, demonstrated that 34% of detected RAPD bands were polymorphic. Of 90 primers tested, 35 showed relatively simple and reliably scorable polymorphisms and were used for segregation analysis. Sixty F2 individuals were scored for the segregation of 73

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Qu, Min. "Astronautical Mutation Breeding in Alfalfa and its Molecular Analysis." Advanced Materials Research 183-185 (January 2011): 2041–45. http://dx.doi.org/10.4028/www.scientific.net/amr.183-185.2041.

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Astronautical mutagenesis is a new breeding technique. In space, the variation are produced by means of the comprehensive effect like high vacuum, microgravity, intense radiation and so on. The mutated seeds by astronautical mutagenesis will be selected to breed the new cultivars. Astronautical mutation breeding opens up a new way for breeding. In this study, the genetic variation of 10 “Zhaodong” Alfalfa mutated lines produced by astronautical mutagenesis were investigated using RAPD method. For RAPD analysis, 90 primers were tested and 21 primers were selected for the detailed RAPD makers. C

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Alam, Riffat Ara, Md Ahashan Habib, and Sheikh Shamimul Alam. "Karyotype and RAPD Analysis in Five Potato Varieties (Solanum tuberosum L.)." Bangladesh Journal of Botany 41, no. 1 (2012): 105–10. http://dx.doi.org/10.3329/bjb.v41i1.11088.

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Two native and three high yielding potato varieties available in Bangladesh were studied cytogenetically at molecular level to get a preliminary idea about their genomes. These varieties possess 2n = 4x = 48 chromosomes. Each variety has definite centromeric formula. Presence of maximum metacentric chromosomes suggested their karyotypes as symmetric type. The range of individual chromosomal and total length of 2n chromosome complements was also distinct in five varieties. Heteromorphicity regarding centromeric position suggesting the occurrence of structural aberration in respective homologous

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Polashock, James J., and Nicholi Vorsa. "Development of SCAR Markers for DNA Fingerprinting and Germplasm Analysis of American Cranberry." Journal of the American Society for Horticultural Science 127, no. 4 (2002): 677–84. http://dx.doi.org/10.21273/jashs.127.4.677.

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DNA fingerprinting has been useful for genotypic classification of American cranberry (Vaccinium macrocarpon Ait.). Polymerase chain reaction (PCR) based methodologies including randomly amplified polymorphic DNA (RAPD) markers are relatively easy to use, and inexpensive as compared to other methods. However, RAPD markers have some limitations including seamless interlaboratory transferability and susceptibility to certain types of error. An alternative method, sequence characterized amplified regions (SCARs), was developed for cranberry germplasm analysis. Nine primer sets were designed from

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Al-Andal, Abeer, Mahmoud Moustafa, and Suliman Alruman. "Taxonomic variation among Schinus molle L. plants associated with a slight change in elevation." Bangladesh Journal of Plant Taxonomy 24, no. 2 (2017): 205–14. http://dx.doi.org/10.3329/bjpt.v24i2.35116.

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This study examined the degree of variations in DNA fingerprints associated with slight altitudinal change of Schinus molle grown in Abha region, Saudi Arabia. Seven populations from Schinus molle plants located at 2193.0, 2246.0, 2197.7, 2441.0, 2372.0, 2250.6 and 2175.0 meters had been investigated. The degree of genetic variability was evaluated using random amplified polymorphic DNA (RAPD), mixed RAPD and intersimple sequence repeat markers (ISSR). The genetic similarity coefficients from RAPD analysis revealed the maximum similarity value (89.9%) was between population at 2250.6 m and pop

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Ganter, Philip F., and Bryan Quarles. "Analysis of population structure of cactophilic yeast from the genusPichia:P.cactophilaandP.norvegensis." Canadian Journal of Microbiology 43, no. 1 (1997): 35–44. http://dx.doi.org/10.1139/m97-006.

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DNAs from 40 strains of Pichia cactophila and Pichia norvegensis, yeasts characteristic of cactus necroses, were compared using randomly amplified polymorphic DNA (RAPD) banding patterns and killer/sensitive phenotypes. Both species belong to the same species complex within the genus. The levels of between-strain RAPD variation were high in both species (higher in the automictic P. cactophila than in the heterothallic P. norvegensis), although there is little variation in physiological abilities within either species. Although each species was a separate lineage, RAPD analysis confirms that th

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Mulyani, Yuniar, Eulis Reni Sundari, Nisa Nur Aidin, Astyani Lesmana, and Aisyah. "Genetic Diversity Analysis of Poeciliidae Fish Using RAPD-PCR Method." Asian Journal of Fisheries and Aquatic Research 24, no. 3 (2023): 44–51. http://dx.doi.org/10.9734/ajfar/2023/v24i3636.

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There are many species of Poeciliidae fish in nature. Poeciliidae is a family of freshwater fish in the order Cyprinodontiformes and are well-known aquarium fish, such as guppies, mollies, platies and swordtails. Therefore, genetic analysis on Poeciliidae is important to collect information about biodiversity, genetic classification, and study developments that occurred during evolution. One approach to determine genetic diversity in many fish species can use Random Amplification Polymorphic DNA (RAPD). This study aims to analyze the genetic diversity of Poeciliidae fish using the RAPD-PCR met

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Suktha, Phansiri, Poramane Chiochan, Atiweena Krittayavathananon, Sangchai Sarawutanukul, Sathyamoorthi Sethuraman, and Montree Sawangphruk. "In situ mass change and gas analysis of 3D manganese oxide/graphene aerogel for supercapacitors." RSC Advances 9, no. 49 (2019): 28569–75. http://dx.doi.org/10.1039/c9ra05444h.

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Manganese oxide nanoparticles decorated on 3D reduced graphene oxide aerogels (3D MnO<sub>x</sub>/rGO<sub>ae</sub>) for neutral electrochemical capacitors were successfully produced by a rapid microwave reduction process within 20 s.

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Vlastníková, H., K. Moravcová, and M. Pidra. "The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones." Horticultural Science 31, No. 4 (2011): 136–39. http://dx.doi.org/10.17221/3807-hortsci.

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Nine identification RAPD markers (Moravcov&aacute; et al. 2003) were used to distinguish 24 clones and grapevine cultivars. No polymorphism was detected among all the tested clones of Chardonnay, Pinot gris and Zweigeltrebe from Pole&scaron;ovice. Pinot noir, Pinot gris, Pinot blanc and Pinot Meunier were indistinguishable within clones, they also showed the identical RAPD profile within cultivars (except discussed sample No. 26). On the other hand, Auxerrois as a relative to cultivars of Pinot group showed unique patterns and may be classified as a different cultivar. Some irregularit

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Okatani, Alexandre Tomomitsu, Hideki Hayashidani, Toshio Takahashi, Takahide Taniguchi, Masuo Ogawa, and Ken-ichi Kaneko. "Randomly Amplified Polymorphic DNA Analysis ofErysipelothrix spp." Journal of Clinical Microbiology 38, no. 12 (2000): 4332–36. http://dx.doi.org/10.1128/jcm.38.12.4332-4336.2000.

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The usefulness of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was studied. Eighty-one strains and 18 random primers were tested. Among the tested primers, the primers NK51 (GGTGGTGGTATC) and NK6 (CCCGCGCCCC) produced noticeable results. The primer NK51 revealed four species-specific RAPD patterns. Of the 66 strains of E. rhusiopathiae, 64 had the same unique band of 884 bp. Of the 12 strains of E. tonsillarum, 11 produced a 1,265-bp band. In addition, two strains, previously thought to be E. rh

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Venieri, D., A. Vantarakis, G. Komninou, and M. Papapetropoulou. "Differentiation of faecal Escherichia coli from human and animal sources by random amplified polymorphic DNA-PCR (RAPD-PCR)." Water Science and Technology 50, no. 1 (2004): 193–98. http://dx.doi.org/10.2166/wst.2004.0053.

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In this study the assessment of randomly amplified polymorphic DNA (RAPD) analysis was established as a molecular epidemiological tool. RAPD analysis was performed to differentiate faecal Escherichia coli isolates from human and animal sources. E. coli strains (128) were isolated from human and animal faeces (from cattle and sheep). Genomic DNA was extracted and randomly amplified polymorphic DNA-PCR (RAPD-PCR) fingerprinting was performed. Seven arbitrary primers were tested with a view to discriminating between E. coli isolates from humans and E. coli isolates from animals. RAPD profiles wer

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Ozbey, G., Ertas HB, and A. Muz. "Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey." Veterinární Medicína 50, No. 12 (2012): 526–30. http://dx.doi.org/10.17221/5660-vetmed.

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Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer (OPG-11). Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT strains were found to have different RAPD profiles. In addition, the RAPD assay showed almost similar DNA profiles among the tested strains of the serotypes A, B, D and E. The strain of serotype C did give a

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Jan, C. H., D. H. Byrne, J. Manhart, and H. Wilson. "Rose Germplasm Analysis with RAPD Markers." HortScience 34, no. 2 (1999): 341–45. http://dx.doi.org/10.21273/hortsci.34.2.341.

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The genus Rosa consists of more than 100 species classified into four subgenera, Eurosa, Platyrhodon, Hesperhodos, and Hulthemia, and distributed widely throughout the northern hemisphere. The subgenus Eurosa includes 11 sections. The other subgenera are monotypic. One hundred and nineteen accessions and 213 markers of 36 rose species that include eight sections of the subgenus Eurosa and one species each from the subgenera Hesperhodos and Platyrhodon were used to calculate a similarity matrix, which was clustered with the unweighted pair group method using arithmetic means (UPGMA). The RAPD m

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Mandal, P. K., I. M. Santha, and S. L. Mehta. "RAPD Analysis of Lathyrus sativus Somaclones." Journal of Plant Biochemistry and Biotechnology 5, no. 2 (1996): 83–86. http://dx.doi.org/10.1007/bf03262987.

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de Wet, Juanita, Michael J. Wingfield, Teresa A. Coutinho, and Brenda D. Wingfield. "Characterization of Sphaeropsis sapinea Isolates from South Africa, Mexico, and Indonesia." Plant Disease 84, no. 2 (2000): 151–56. http://dx.doi.org/10.1094/pdis.2000.84.2.151.

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Post-hail-associated dieback of Pinus patula and P. radiata trees, induced by Sphaeropsis sapinea (=Diplodia pinea), is a common and important disease in commercial pine plantations. Two morphotypes, A and B, have been described for this fungus based on differences in cultural characteristics, conidial morphology, and virulence among isolates from the north central United States. The existence of the two described morphotypes was later verified through the use of random amplified polymorphic DNA (RAPD) analyses, and the morphotypes were designated as the A and B RAPD marker groups. The objecti

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Servienė, Elena, Irena Kemežienė, Jūratė Kasperovičienė, Brigita Čapukoitienė, Vida Rančelienė, and Judita Koreivienė. "Optimization of DNA isolation and PCR parameters for RAPD analysis of Gonyostomum semen (Raphidophyceae)." Botanica Lithuanica 18, no. 1 (2012): 40–45. http://dx.doi.org/10.2478/v10279-012-0006-6.

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Abstract Servienė E., Kemežienė I., Kasperovičienė J., čapukoitienė B., Rančelienė V., Koreivienė J., 2012: Optimization of DNA isolation and PCR parameters for RAPD analysis of Gonyostomum semen (Raphidophyceae) [DNR izoliavimas ir PGR parametrų optimizavimas Gonyostomum semen (Raphidophyceae) dumblių RAPD analizei]. - Bot. Lith., 18(1): 40-45. The genomic DNA purification method for Gonyostomum semen algae was optimized by applying different DNA purification techniques and rational modifications. This method allowed to obtain high quality DNA preparations suitable for the phylogenetic analys

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Vu-Thien, Hoang, Carole Dulot, Didier Moissenet, Brigitte Fauroux, and Antoine Garbarg-Chenon. "Comparison of Randomly Amplified Polymorphic DNA Analysis and Pulsed-Field Gel Electrophoresis for Typing of Moraxella catarrhalis Strains." Journal of Clinical Microbiology 37, no. 2 (1999): 450–52. http://dx.doi.org/10.1128/jcm.37.2.450-452.1999.

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Randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) for the analysis of 13 Moraxella catarrhalis isolates, 11 successive strains isolated from sputa of five children and 2 isolates obtained the same day from twins, were compared. RAPD and PFGE both yielded nine types from the 13 isolates, showing a chronic colonization with one strain in three patients and a successive colonization with different strains in two patients. The promising results obtained with RAPD should be confirmed with a larger number of strains, but RAPD seems as suitable as PFGE for the typi

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Wettasinghe, Ruwanthi C., and Ellen B. Peffley. "Optimizing RAPD Markers for Onion Genomic DNA Analysis." HortScience 30, no. 4 (1995): 877E—877. http://dx.doi.org/10.21273/hortsci.30.4.877e.

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Random amplified polymorphic DNA (RAPD) have potential as genetic markers that may facilitate selection in plant improvement. To obtain clear, reproducible, and repeatable RAPD bands, four DNA extraction protocols and two Taq polymerases were compared. DNA extraction followed modified Tai and Tanksley (PMBR), Dellaporta et al. (PMBR), and Guilllemant et al. (PMBR) protocols, and a plant tissue DNA isolation kit from Gentra Systems was used. The modified Guillemant protocol was selected because of ease of extraction and cost effectiveness. Genotypes studied were TG1015Y (Allium cepa). Polymeras

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Okan, Kemalcan, Zekiye Özkara, Emre Sevindik, Mustafa Sevindik, and Yavuz Paksoy. "Molecular markers analysis of endemic Bornmuellera hausskn. spp. (Brassicaceae) in Türkiye." Genetika 56, no. 2 (2024): 295–304. http://dx.doi.org/10.2298/gensr2402295o.

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In this study, molecular characterisation of T?rkiye's endemic species Bornmuellera cappadocica (Willd.) Cullen & T.R. Dudley, Bornmuellera glabrescens (Boiss. & Balansa) Cullen & T.R. Dudley, Bornmuellera kiyakii Ayta? & Aksoy and Bornmuellera angustifolia (Hausskn. ex Bornm.) Cullen & T.R.Dudley was carried out using ten RAPD and ten ISSR primers. In RAPD-PCR analysis, 66 bands were obtained and the polymorphism rate was 96.96%. In the ISSR-PCR analysis, 119 bands were obtained and the polymorphism rate was 95.79%. In the UPGMA (Unweighted Pair Group Method with Arithmati

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