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Rozprawy doktorskie na temat „Real-time cell analysis”

Autor: Grafiati

Data publikacji: 9 listopada 2024

Data aktualizacji: 31 lipca 2025

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1

Hong, Soonjin Barbee Kenneth A. "Quantitative analysis of cell-surface interactions and cell adhesion process in real-time /." Philadelphia, Pa. : Drexel University, 2008. http://hdl.handle.net/1860/2840.

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Schulz, Craig. "Microsequential injection systems for the real-time monitoring of glucose metabolism of live cells by enzymatic assay /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/8694.

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Maiuri, Paolo. "Single-cell and real-time analysis of transcription rates from integrated HIV-1 provirus." Doctoral thesis, Scuola Normale Superiore, 2009. http://hdl.handle.net/11384/85935.

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Viral RNA biogenesis is a crucial step in the replication of retroviruses that require both the production of a genomic RNA as well as of translation templates. Cellular and viral factors concur in the biogenesis of RNA at the specific sub-nuclear chromatin site where the reaction takes place. The possibility of tracking viral RNA in living cells gives the unique possibility of measuring the kinetic parameters of RNA biogenesis as well as defining the dynamic recruitment of host and viral factors to the site of replication. In order to study the activation of HIV-1 gene expression from the int

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Shoshi, Astrit [Verfasser]. "Magnetic lab-on-a-chip for cell analysis : magnetoresistive-based real-time monitoring of dynamic cell-environment interactions / Astrit Shoshi." Bielefeld : Universitaetsbibliothek Bielefeld, 2013. http://d-nb.info/1036974502/34.

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Li, Min. "Kinetic analysis of Human T-cell leukemia virus type 1 gene expression." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1228156327.

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Zhou, Daming. "Modeling and Multi-Dimensional Analysis of a Proton Exchange Membrane Fuel Cell." Thesis, Bourgogne Franche-Comté, 2017. http://www.theses.fr/2017UBFCA011/document.

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Un des freins à la commercialisation de masse de la pile à combustible et notamment de la technologie à membrane échangeuse de proton vient de sa faible durée de vie due à la difficulté de contrôler le système sous certaines conditions. Pour pallier à ce problème, l’élaboration d’un modèle mathématique précis de la pile à combustible à membrane échangeuse de protons permettant d’observer les variables internes et l'état de la pile à combustible au cours de son fonctionnement permettrait le développement de la stratégie de contrôle du système.Cette thèse propose d’élaborer un modèle dynamique m

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Czerwieniec, Gregg Allen. "Single cell analysis using bio-aerosol mass spectrometry : development and applications for the real-time detection of bio-warfare pathogens /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.

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AL, DOSSARY REEM. "Activation of human endogenous retrovirus K and cellular modifications in human melanoma cell lines: gene expression analysis." Doctoral thesis, Università degli Studi di Roma "Tor Vergata", 2010. http://hdl.handle.net/2108/1389.

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Presso i laboratori dell’Università di Tor Vergata è stato sviluppato un sistema in vitro dotato di caratteristiche idonee allo studio dei meccanismi che sono alla base dello sviluppo e della progressione del melanoma. Tale sistema è costituito da una linea cellulare di melanoma umano, isolata da una lesione metastatica di una paziente e caratterizzata dalla capacità di crescere in adesione (linea TVM-A12), da cui sono state ottenute due distinte linee cellulari, dotate invece della caratteristica di crescita in sospensione, derivate l’una mediante tecnica della diluizione limite (Clonesp), e

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Boussemaere, Luc. "Investigating off-axis digital holographic microscopy with a source of partial spatial coherence as a real-time sensor for cell cultures." Doctoral thesis, Universite Libre de Bruxelles, 2015. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/209086.

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Bio-pharmaceutical industry is a vast growing market and recent recommendations of the Food and Drug Administration have put a large emphasis on the characterization of biological processes and models. As a consequence, there is a high incentive on developing modern sensors in order to more accurately monitor and control processes. In that way, Digital Holographic Microscopy (DHM) presents unique features thanks to the refocusing and quantitative phase contrast imaging capabilities. In this thesis we investigate the usage of DHM to monitor yeast cultures that are often used in both the bio-pha

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Pretorius, Ashley. "Functional analysis of the mouse RBBP6 gene using Interference RNA." Thesis, University of the Western Cape, 2007. http://etd.uwc.ac.za/index.php?module=etd&action=viewtitle&id=gen8Srv25Nme4_4435_1264363734.

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<p>The aim of this thesis was to investigate the cellular role of the mouse RBBP6 gene using the interference RNA (RNAi) gene targeting technology and also to understand the relevance of two promoters for the RBBP6 gene.</p>

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Thompson, Bruce Thomas. "Fourier transform infrared spectroscopic measurement of carbon monoxide and nitric oxide in sidestream cigarette smoke in real time using a hollow waveguide gas cell and nonimaging optics." Diss., Available online, Georgia Institute of Technology, 2004:, 2004. http://etd.gatech.edu/theses/available/etd-06232004-172923/unrestricted/thompson%5Fbruce%5Ft%5F200407%5Fphd.pdf.

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Thesis (Ph. D.)--Chemistry and Biochemistry, Georgia Institute of Technology, 2005. Directed by Boris Mizaikoff.<br>Hunt, William, Committee Member ; Weck, Marcus, Committee Member ; Mizaikoff, Boris, Committee Chair ; Janata, Jiri, Committee Member ; Orlando, Thomas, Committee Member. Includes bibliographical references.

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Du, Toit Jean. "An analysis of the effect of transformation on global– and gene–specific DNA methylation in four cultured cell lines / Jean du Toit." Thesis, North-West University, 2010. http://hdl.handle.net/10394/4560.

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DNA methylation plays a role in several biological functions, such as gene expression regulation, and several endogenous and exogenous factors affect these DNA methylation patterns in the cell. One such alteration of a cell line's DNA methylation pattern is caused by the insertion of a vector into the cell line. Using the cytosine–extension assay and realtime methylation–specific PCR, alterations of DNA methylation levels on both global and gene–specific levels were investigated. In some cell lines the cellular transformation led to an increase in DNA methylation levels, and in others a decrea

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Normand, Camille. "La rhinοpneumοnie équine : caractérisatiοn mοléculaire et cellulaire de l'herpèsvirus équin 4, un mοdèle d'étude pοur l'appοrt de cοnnaissances dans la pathοgénie de la maladie, la survie et l'intégrité du virus ainsi que l'identificatiοn de mοlécules antivirales". Electronic Thesis or Diss., Normandie, 2024. http://www.theses.fr/2024NORMC405.

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Jusqu’en 1981, l’herpèsvirus équin 4 (HVE-4) et l’HVE-1, responsables de la rhinopneumonie, étaient considérés comme deux sous-types d’un même virus. Il a depuis été démontré que l’HVE-4 était très peu impliqué dans les avortements et son implication dans la forme nerveuse de la maladie n’est pas démontrée à ce jour. C’est probablement la raison qui fait que ce virus est moins étudié que l’HVE-1. Ce travail de thèse a porté sur trois aspects importants de la rhinopneumonie chez le cheval afin d’apporter de la connaissance sur l’HVE-4 et d’évaluer l’intérêt d’utiliser ce virus comme modèle. Nou

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Abrahams, Beynon. "The effects of various combinations of different classes of anticancer drugs and tyrosine kinase inhibitors on the human MCF-7 breast carcinoma cell line." Thesis, University of the Western Cape, 2014. http://hdl.handle.net/11394/3846.

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Magister Scientiae (Medical Bioscience) - MSc(MBS)<br>This study investigated the effects of TKIs on the growth and proliferation of MCF-7 breast carcinoma cells in culture. MCF-7 cells were exposed to different concentrations of TKIs alone and in combination with each other. Inhibition of cell growth by TKIs used individually occurred in a dose- and time-dependent manner. When EGFR Inhibitor I, EGFR Inhibitor II/BIBX1382 and the multi-specific EGFR/ErbB-2/ErB-4 Inhibitor were used in combination with each other at equimolar log dose concentrations, the combined effects on cell growth was sign

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Xavier, Flavia Dias. "Padrão de expressão e significado prognóstico dos genes BCL2, BCL6, CCND2, FN1, LMO2 e SCYA3 pela técnica de PCR em tempo real com linfoma difuso de grandes células B tratado com rituximabe." Universidade de São Paulo, 2013. http://www.teses.usp.br/teses/disponiveis/5/5167/tde-24062013-114437/.

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Introdução: O linfoma difuso de grandes células B é o mais freqüente grupo de linfoma não- Hodgkin, perfazendo quase 50% dos casos no serviço de hematologia do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo e Instituto do Câncer do Estado de São Paulo. Possui heterogeneidade clínica e biológica traduzida em mais de vinte subtipos na Organização Mundial da Saúde. Sua terapêutica se baseia na associação do anticorpo monoclonal anti-CD20 e quimioterapia com antracíclico, esquema que resulta em 43,5% de sobrevida global em 10 anos. Determinantes de prognóstico clínico

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Puebla-Osorio, Nahum. "Transcriptional analysis of chicken immune cells following exposure to 2,3,7,8,-tetrachlorodibenzo-p-dioxin (TCDD)." Texas A&M University, 2004. http://hdl.handle.net/1969.1/3165.

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In the present investigation, microarray analysis was used to identify potential TCDD gene targets. Three microarray experiments were performed to study the effect of TCDD in an established chicken B-cell line (DT40), in a chicken macrophage cell line (HD11), and in the bursa of Fabricius from embryos exposed in ovo at 6 days of incubation. From the DT40 microarray analyses, clones with sequence similarity to the apoptotic genes caspase 8 and caspase 9, and the transcription factor NFΜB, among others, were identified. Real-time quantitative polymerase chain reaction (RT-PCR) revealed that

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Giedt, Randy James. "Real-Time Acquisition and Analysis of Endothelial Mitochondrial Superoxide Radical Production and Membrane Potential During In Vitro Ischemia/Reperfusion." The Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1243541457.

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Albuquerque, Andreia de, Sepp Kaul, Georg Breier, Petra Krabisch, and Nikos Fersis. "Multimarker Analysis of Circulating Tumor Cells in Peripheral Blood of Metastatic Breast Cancer Patients: A Step Forward in Personalized Medicine." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-136627.

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Aim: To develop an immunomagnetic assay for the isolation of circulating tumor cells (CTCs) followed by the analysis of a multimarker panel, which will enable the characterization of these malignant cells with high accuracy. Patients and Methods: Peripheral blood (PB) was collected from 32 metastatic breast cancer patients and 42 negative controls. The antibodies BM7 and VU1D9 were used for immunomagnetic tumor cell enrichment. A real-time reverse transcription-polymerase chain reaction (RT-PCR) approach for the markers KRT19, SCGB2A2, MUC1, EPCAM, BIRC5 and ERBB2 was used for CTC detection an

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Albuquerque, Andreia de, Sepp Kaul, Georg Breier, Petra Krabisch, and Nikos Fersis. "Multimarker Analysis of Circulating Tumor Cells in Peripheral Blood of Metastatic Breast Cancer Patients: A Step Forward in Personalized Medicine." Karger, 2012. https://tud.qucosa.de/id/qucosa%3A27718.

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Aim: To develop an immunomagnetic assay for the isolation of circulating tumor cells (CTCs) followed by the analysis of a multimarker panel, which will enable the characterization of these malignant cells with high accuracy. Patients and Methods: Peripheral blood (PB) was collected from 32 metastatic breast cancer patients and 42 negative controls. The antibodies BM7 and VU1D9 were used for immunomagnetic tumor cell enrichment. A real-time reverse transcription-polymerase chain reaction (RT-PCR) approach for the markers KRT19, SCGB2A2, MUC1, EPCAM, BIRC5 and ERBB2 was used for CTC detection an

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Zikmund, Tomáš. "Matematické metody pro zpracování obrazu v biologických pozorováních." Doctoral thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2014. http://www.nusl.cz/ntk/nusl-234208.

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The dissertation deals with the image processing in digital holographic microscopy and X-ray computed tomography. The focus of the work lies in the proposal of data processing techniques to meet the needs of the biological experiments. Transmitted light holographic microscopy is particularly used for quantitative phase imaging of transparent microscopic objects such as living cells. The phase images are affected by the phase aberrations that make the analysis particularly difficult. Here, we present a novel algorithm for dynamical processing of living cells phase images in a time-lapse sequenc

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de, Albuquerque Andreia, Ilja Kubisch, Georg Breier, et al. "Multimarker Gene Analysis of Circulating Tumor Cells in Pancreatic Cancer Patients: A Feasibility Study." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2014. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-133530.

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Objective: The aim of this study was to develop an immunomagnetic/real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay and assess its clinical value for the molecular detection of circulating tumor cells (CTCs) in peripheral blood of pancreatic cancer patients. Methods: The presence of CTCs was evaluated in 34 pancreatic cancer patients before systemic therapy and in 40 healthy controls, through immunomagnetic enrichment, using the antibodies BM7 and VU1D9 [targeting mucin 1 and epithelial cell adhesion molecule (EpCAM), respectively], followed by real-time RT-PCR analysis

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de, Albuquerque Andreia, Ilja Kubisch, Georg Breier, et al. "Multimarker Gene Analysis of Circulating Tumor Cells in Pancreatic Cancer Patients: A Feasibility Study." Karger, 2012. https://tud.qucosa.de/id/qucosa%3A27513.

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Objective: The aim of this study was to develop an immunomagnetic/real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay and assess its clinical value for the molecular detection of circulating tumor cells (CTCs) in peripheral blood of pancreatic cancer patients. Methods: The presence of CTCs was evaluated in 34 pancreatic cancer patients before systemic therapy and in 40 healthy controls, through immunomagnetic enrichment, using the antibodies BM7 and VU1D9 [targeting mucin 1 and epithelial cell adhesion molecule (EpCAM), respectively], followed by real-time RT-PCR analysis

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PIZZOLATO, ALBERTO. "Topology optimization for energy problems." Doctoral thesis, Politecnico di Torino, 2018. http://hdl.handle.net/11583/2710567.

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The optimal design of energy systems is a challenge due to the large design space and the complexity of the tightly-coupled multi-physics phenomena involved. Standard design methods consider a reduced design space, which heavily constrains the final geometry, suppressing the emergence of design trends. On the other hand, advanced design methods are often applied to academic examples with reduced physics complexity that seldom provide guidelines for real-world applications. This dissertation offers a systematic framework for the optimal design of energy systems by coupling detailed physical an

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Guidi, Mònica. "Micro RNA-Mediated regulation of the full-length and truncated isoforms of human neurotrophic tyrosine kinase receptor type 3 (NTRK 3)." Doctoral thesis, Universitat Pompeu Fabra, 2009. http://hdl.handle.net/10803/7114.

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Neurotrophins and their receptors are key molecules in the development of the<br/>nervous system. Neurotrophin-3 binds preferentially to its high-affinity receptor<br/>NTRK3, which exists in two major isoforms in humans, the full-length kinaseactive<br/>form (150 kDa) and a truncated non-catalytic form (50 kDa). The two<br/>variants show different 3'UTR regions, indicating that they might be differentially<br/>regulated at the post-transcriptional level. In this work we explore how<br/>microRNAs take part in the regulation of full-length and truncated NTRK3,<br/>demonstrating that the two isof

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Lu, Chun-Han, and 呂俊翰. "Real-time Fluorescence Detection System for Cell Analysis." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/58921215391122022950.

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碩士<br>國立屏東科技大學<br>車輛工程系所<br>103<br>In this study, a real-time fluorescence detection system for cell growth analysis is proposed utilizing the CMOS (Complementary Metal-Oxide-Semiconductor) modules with blue LEDs. The RAW 264.7 murine macrophage cell labels with CFSE (Carboxyfluorescein succinimidyl ester) fluorescence dye and the blue LEDs are for cells fluorescence excitation for real time cell growth fluorescence monitoring. The CMOS modules and LEDs are controlled using custom-made LABVIEW code to capture real time cell growth fluorescence images automatically. The cell growth images are a

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Hsu, Che Wei, and 許哲瑋. "Development of a microfluidic chip for perfusion three-dimensional cell culture and real-time dynamic cell growth analysis." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/23123445306536155343.

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碩士<br>長庚大學<br>醫療機電工程研究所<br>101<br>A perfusion three dimensional (3D) cell culture microfluidic chip has been developed for the real-time and non-invasive impedimetric monitoring of cell dynamics. The chip can continuously estimate the cell proliferation or chemosensitivity under different culture conditions. It is capable of onsite detecting the cell number or viability in the 3D cell culture construct without sacrificing the cultured cells. The chip consists of a substrate, a cover layer and a fluidic layer. The chip substrate is a glass substrate and a pair of 3D vertical electrodes is fabri

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Chang, Chia-Hao, and 張家豪. "Evaluation of digital real-time PCR assay as a molecular diagnostic tool for single-cell analysis." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/ycm2d3.

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博士<br>國立臺灣大學<br>高分子科學與工程學研究所<br>106<br>In a single-cell study, isolating and identifying single cells are essential, but these processes often require a large investment of time or money. The aim of this study was to isolate and analyse single cells using a novel platform, the PanelChip™ Analysis System, which includes 2500 microwells chip and a digital real-time polymerase chain reaction (dqPCR) assay, in comparison with a standard RT-PCR (qPCR) assay. Through the serial dilution of a known concentration standard, namely pUC19, the accuracy and sensitivity levels of two methodologies were com

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Malik, Neelam. "Gene expression analysis of squamous cell carcinoma of the oesophagus using a novel real time PCR probe system." Thesis, 2010. http://hdl.handle.net/10413/706.

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Squamous cell carcinoma of the oesophagus (OSCC) is a common malignancy that occurs with high frequency in certain parts of the world, including South Africa. The aetiology of OSCC has remained unclear although many studies suggest that it is caused by a combination of variable risk factors. Recent reports implicate a variety of genetic factors in the carcinogenesis of OSCC but their involvement is yet to be defined.<br>Thesis (M.Med)-University of KwaZulu-Natal, Durban, 2010.

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Yuan, Fan. "Optimization of microelectrode sensor sensitivity for real-time monitoring important physiological parameters of human renal epithelial cell." Thesis, 2020. https://hdl.handle.net/2144/40715.

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In order to calculate specific impedance of cell-covered electrodes in a Equation of morphological parameters of cell per se, an ECIS model of Human Renal Epithelial Cell are created by analysis partial differential equations describing three intrinsic pathways of electrical currents in the system. Based on this cell model, this research explores how some adjustable dimensional parameters of electrode-configuration impact sensor sensitivity by changing the overall impedance contribution of electrical double layer. Namely, it includes electrode planner area, spacing between working and counter

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LIN, YU-JEN, and 林俞任. "Investigation on Equivalent Circuit Analysis of Large-area Arrayed TiO2/ZnO Dye-sensitized Solar Cell Modified by Graphene Integrated with Magnetic Beads and Study on Wireless-based Remote Real-time Monitoring System." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/rsa2ju.

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Kuo, Chien-Hung, and 郭建宏. "Investigation on Photovoltaic Properties of Flexible Arrayed Dye-sensitized Solar Cell Based on IGZO/ TiO2 Double Layered Structure Modified by Graphene under the Low Illumination and Study on Impedance Analysis and Wireless-based Remote Real-time Monitoring System." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/a6xv27.

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碩士<br>國立雲林科技大學<br>電子工程系<br>106<br>In this thesis, a way to improve the photovoltaic conversion efficiency (η) of dye-sensitized solar cell (DSSC) has been provided. The structure was divided into two parts. In the first part, the reduced graphene oxide (RGO) - TiO2 composite was fabricated by using hydro-thermal method, which was acted as the dye - adsorbed layer. In the second part, the indium gallium zinc oxide (IGZO) was deposited between dye-adsorbed layer and electrolyte by using sputter system. The DSSC was investigated by electrochemical impedance spectroscopy (EIS), sun light simulatio

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Shimoga, Muddappa Vinay Kumar. "Electrochemical model based condition monitoring of a Li-ion battery using fuzzy logic." Thesis, 2014. http://hdl.handle.net/1805/5588.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>There is a strong urge for advanced diagnosis method, especially in high power battery packs and high energy density cell design applications, such as electric vehicle (EV) and hybrid electric vehicle segment, due to safety concerns. Accurate and robust diagnosis methods are required in order to optimize battery charge utilization and improve EV range. Battery faults cause significant model parameter variation affecting battery internal states and output. This work is focused on developing diagnosis method to reliably detect various

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