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Artykuły w czasopismach na temat „Related organelles”

Autor: Grafiati

Data publikacji: 2 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Dell'Angelica, Esteban C., Chris Mullins, Steve Caplan, and Juan S. Bonifacino. "Lysosome‐related organelles." FASEB Journal 14, no. 10 (2000): 1265–78. http://dx.doi.org/10.1096/fasebj.14.10.1265.

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DELL'ANGELICA, E. C. "Lysosome-related organelles." FASEB Journal 14, no. 10 (2000): 1265–78. http://dx.doi.org/10.1096/fj.14.10.1265.

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Cutler, D. F. "Introduction: lysosome-related organelles." Seminars in Cell & Developmental Biology 13, no. 4 (2002): 261–62. http://dx.doi.org/10.1016/s108495210200054x.

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Smith, R. S., and W. S. Kendal. "The recovery of organelle transport and microtubule integrity in myelinated axons that are frozen and thawed." Canadian Journal of Physiology and Pharmacology 63, no. 4 (1985): 292–97. http://dx.doi.org/10.1139/y85-053.

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Myelinated axons of Xenopus laevis were rapidly frozen in liquid nitrogen and thawed in a potassium glutamate based medium. Organelles within isolated, thawed axons were visualized by light microscopy. After thawing, organelles were stationary for about 5 min. Following this quiescent period, organelles exhibited a low frequency oscillation in the longitudinal direction of the axon; some of the organelles then began to move in either the anterograde or retrograde directions. Electron microscopic examination of axonal cross sections showed that few microtubules were present immediately after th

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Okamoto, Koji. "Organellophagy: Eliminating cellular building blocks via selective autophagy." Journal of Cell Biology 205, no. 4 (2014): 435–45. http://dx.doi.org/10.1083/jcb.201402054.

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Maintenance of organellar quality and quantity is critical for cellular homeostasis and adaptation to variable environments. Emerging evidence demonstrates that this kind of control is achieved by selective elimination of organelles via autophagy, termed organellophagy. Organellophagy consists of three key steps: induction, cargo tagging, and sequestration, which involve signaling pathways, organellar landmark molecules, and core autophagy-related proteins, respectively. In addition, posttranslational modifications such as phosphorylation and ubiquitination play important roles in recruiting a

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Ci, Yali, and Lei Shi. "Compartmentalized replication organelle of flavivirus at the ER and the factors involved." Cellular and Molecular Life Sciences 78, no. 11 (2021): 4939–54. http://dx.doi.org/10.1007/s00018-021-03834-6.

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AbstractFlaviviruses are positive-sense single-stranded RNA viruses that pose a considerable threat to human health. Flaviviruses replicate in compartmentalized replication organelles derived from the host endoplasmic reticulum (ER). The characteristic architecture of flavivirus replication organelles includes invaginated vesicle packets and convoluted membrane structures. Multiple factors, including both viral proteins and host factors, contribute to the biogenesis of the flavivirus replication organelle. Several viral nonstructural (NS) proteins with membrane activity induce ER rearrangement

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Higuchi, Yujiro. "Membrane traffic related to endosome dynamics and protein secretion in filamentous fungi." Bioscience, Biotechnology, and Biochemistry 85, no. 5 (2021): 1038–45. http://dx.doi.org/10.1093/bbb/zbab004.

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ABSTRACTIn eukaryotic cells, membrane-surrounded organelles are orchestrally organized spatiotemporally under environmental situations. Among such organelles, vesicular transports and membrane contacts occur to communicate each other, so-called membrane traffic. Filamentous fungal cells are highly polarized and thus membrane traffic is developed to have versatile functions. Early endosome (EE) is an endocytic organelle that dynamically exhibits constant long-range motility through the hyphal cell, which is proven to have physiological roles, such as other organelle distribution and signal tran

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Figon, Florent, Ilse Hurbain, Xavier Heiligenstein, et al. "Catabolism of lysosome-related organelles in color-changing spiders supports intracellular turnover of pigments." Proceedings of the National Academy of Sciences 118, no. 35 (2021): e2103020118. http://dx.doi.org/10.1073/pnas.2103020118.

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Pigment organelles of vertebrates belong to the lysosome-related organelle (LRO) family, of which melanin-producing melanosomes are the prototypes. While their anabolism has been extensively unraveled through the study of melanosomes in skin melanocytes, their catabolism remains poorly known. Here, we tap into the unique ability of crab spiders to reversibly change body coloration to examine the catabolism of their pigment organelles. By combining ultrastructural and metal analyses on high-pressure frozen integuments, we first assess whether pigment organelles of crab spiders belong to the LRO

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Lu, Sha, Zhiqi Dai, Yunxi Cui, and De-Ming Kong. "Recent Development of Advanced Fluorescent Molecular Probes for Organelle-Targeted Cell Imaging." Biosensors 13, no. 3 (2023): 360. http://dx.doi.org/10.3390/bios13030360.

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Fluorescent molecular probes are very powerful tools that have been generally applied in cell imaging in the research fields of biology, pathology, pharmacology, biochemistry, and medical science. In the last couple of decades, numerous molecular probes endowed with high specificity to particular organelles have been designed to illustrate intracellular images in more detail at the subcellular level. Nowadays, the development of cell biology has enabled the investigation process to go deeply into cells, even at the molecular level. Therefore, probes that can sketch a particular organelle’s loc

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Liu, Fangfang, Seng Kah Ng, Yanfen Lu, Wilson Low, Julian Lai, and Gregory Jedd. "Making two organelles from one: Woronin body biogenesis by peroxisomal protein sorting." Journal of Cell Biology 180, no. 2 (2008): 325–39. http://dx.doi.org/10.1083/jcb.200705049.

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Woronin bodies (WBs) are dense-core organelles that are found exclusively in filamentous fungi and that seal the septal pore in response to wounding. These organelles consist of a membrane-bound protein matrix comprised of the HEX protein and, although they form from peroxisomes, their biogenesis is poorly understood. In Neurospora crassa, we identify Woronin sorting complex (WSC), a PMP22/MPV17-related membrane protein with dual functions in WB biogenesis. WSC localizes to large peroxisome membranes where it self-assembles into detergent-resistant oligomers that envelop HEX assemblies, produc

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Wang, Meng. "INTER-ORGANELLE CROSSTALK IN THE REGULATION OF AGING AND LONGEVITY." Innovation in Aging 7, Supplement_1 (2023): 340. http://dx.doi.org/10.1093/geroni/igad104.1132.

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Abstract In eukaryotic cells, specific functions are compartmentalized into various organelles that are evolutionarily conserved across species. Many of these organelles play crucial roles in regulating cellular homeostasis and organismal health. Lysosomes, mitochondria, lipid droplets, and endoplasmic reticulum (ER) are highly metabolic active and support the metabolic balance of lipids, amino acids, carbohydrates, and nucleotides. These organelles also regulate signal transduction, transcriptional responses, and epigenetic dynamics, and contribute to the control of lifespan and healthspan in

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YENER, Yeşim, Mustafa YILMAZ, Burak KIRAS, and Mert ŞEN. "Preservice science teachers’ cognitive structures regarding the cell and its organelles." Acta Didactica Napocensia 17, no. 1 (2024): 178–97. http://dx.doi.org/10.24193/adn.17.1.14.

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Learning the cell, one of the basic subjects of science education, by preservice teachers in a comprehensive way is important for making sense of its vital activities and teaching the subject in a meaningful way in the future. The study aimed to determine preservice science teachers' cognitive structures related to the cell and its organelles before and after the Biology course. The study group consists of 32 preservice science teachers. The single-group interrupted time series design was adopted in the study. Data were collected through the word association test. As a result of the data analy

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Morgan, Anthony J. "Ca2+ dialogue between acidic vesicles and ER." Biochemical Society Transactions 44, no. 2 (2016): 546–53. http://dx.doi.org/10.1042/bst20150290.

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Extracellular stimuli evoke the synthesis of intracellular second messengers, several of which couple to the release of Ca2+ from Ca2+-storing organelles via activation of cognate organellar Ca2+-channel complexes. The archetype is the inositol 1,4,5-trisphosphate (IP3) and IP3 receptor (IP3R) on the endoplasmic reticulum (ER). A less understood, parallel Ca2+ signalling cascade is that involving the messenger nicotinic acid adenine dinucleotide phosphate (NAADP) that couples to Ca2+ release from acidic Ca2+ stores [e.g. endo-lysosomes, secretory vesicles, lysosome-related organelles (LROs)].

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14

Shiflett, April M., and Patricia J. Johnson. "Mitochondrion-Related Organelles in Eukaryotic Protists." Annual Review of Microbiology 64, no. 1 (2010): 409–29. http://dx.doi.org/10.1146/annurev.micro.62.081307.162826.

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Zayani, Zoofa, Arash Matinahmadi, Alireza Tavakolpournegari, and Seyed Hesamoddin Bidooki. "Exploring Stressors: Impact on Cellular Organelles and Implications for Cellular Functions." Stresses 5, no. 2 (2025): 26. https://doi.org/10.3390/stresses5020026.

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Cellular stressors have been demonstrated to exert a substantial influence on the functionality of organelles, thereby impacting cellular homeostasis and contributing to the development of disease pathogenesis. This review aims to examine the impact of diverse stressors, including environmental, chemical, biological, and physical factors, on critical organelles such as the cell membrane, mitochondria, endoplasmic reticulum, Golgi apparatus, lysosomes, and membrane-less organelles. The intricate molecular mechanisms underlying cellular stress responses, encompassing oxidative stress, protein mi

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16

Gasparotto, Matteo, Yi-Shin Lee, Alessandra Palazzi, Marcella Vacca, and Francesco Filippini. "Nuclear and Cytoplasmatic Players in Mitochondria-Related CNS Disorders: Chromatin Modifications and Subcellular Trafficking." Biomolecules 12, no. 5 (2022): 625. http://dx.doi.org/10.3390/biom12050625.

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Aberrant mitochondrial phenotypes are common to many central nervous system (CNS) disorders, including neurodegenerative and neurodevelopmental diseases. Mitochondrial function and homeostasis depend on proper control of several biological processes such as chromatin remodeling and transcriptional control, post-transcriptional events, vesicle and organelle subcellular trafficking, fusion, and morphogenesis. Mutation or impaired regulation of major players that orchestrate such processes can disrupt cellular and mitochondrial dynamics, contributing to neurological disorders. The first part of t

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Di Mattia, Thomas, Catherine Tomasetto, and Fabien Alpy. "A Third Musketeer on the ER: MOSPD2 is a Novel VAP-related Receptor for FFAT Motifs." Contact 1 (January 2018): 251525641880973. http://dx.doi.org/10.1177/2515256418809730.

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Interorganelle membrane contact sites are subcellular structures that favor exchange and communication inside the cell. Such microdomains are built by molecular bridges that create a physical connection between two distinct organelles. The field of contact sites is now flourishing with discoveries of new tethering molecules. In that context, we identified by an unbiased proteomic approach a novel scaffold protein named MOtile SPerm Domain-containing protein 2 (MOSPD2). MOSPD2 is an endoplasmic reticulum (ER)-resident protein that is able to interact with several organelle-bound proteins that p

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Picca, Anna, Riccardo Calvani, Hélio José Coelho-Junior, Francesco Landi, Roberto Bernabei, and Emanuele Marzetti. "Inter-Organelle Membrane Contact Sites and Mitochondrial Quality Control during Aging: A Geroscience View." Cells 9, no. 3 (2020): 598. http://dx.doi.org/10.3390/cells9030598.

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Mitochondrial dysfunction and failing mitochondrial quality control (MQC) are major determinants of aging. Far from being standalone organelles, mitochondria are intricately related with cellular other compartments, including lysosomes. The intimate relationship between mitochondria and lysosomes is reflected by the fact that lysosomal degradation of dysfunctional mitochondria is the final step of mitophagy. Inter-organelle membrane contact sites also allow bidirectional communication between mitochondria and lysosomes as part of nondegradative pathways. This interaction establishes a function

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Kim, Jinoh, and Hua Bai. "Peroxisomal Stress Response and Inter-Organelle Communication in Cellular Homeostasis and Aging." Antioxidants 11, no. 2 (2022): 192. http://dx.doi.org/10.3390/antiox11020192.

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Peroxisomes are key regulators of cellular and metabolic homeostasis. These organelles play important roles in redox metabolism, the oxidation of very-long-chain fatty acids (VLCFAs), and the biosynthesis of ether phospholipids. Given the essential role of peroxisomes in cellular homeostasis, peroxisomal dysfunction has been linked to various pathological conditions, tissue functional decline, and aging. In the past few decades, a variety of cellular signaling and metabolic changes have been reported to be associated with defective peroxisomes, suggesting that many cellular processes and funct

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20

Hermann, Greg J., Lena K. Schroeder, Caroline A. Hieb, et al. "Genetic Analysis of Lysosomal Trafficking inCaenorhabditis elegans." Molecular Biology of the Cell 16, no. 7 (2005): 3273–88. http://dx.doi.org/10.1091/mbc.e05-01-0060.

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The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other gl

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21

Collier, Sophie L., Sarah N. Farrell, Christopher D. Goodman, and Geoffrey I. McFadden. "Modes and mechanisms for the inheritance of mitochondria and plastids in pathogenic protists." PLOS Pathogens 21, no. 1 (2025): e1012835. https://doi.org/10.1371/journal.ppat.1012835.

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Pathogenic protists are responsible for many diseases that significantly impact human and animal health across the globe. Almost all protists possess mitochondria or mitochondrion-related organelles, and many contain plastids. These endosymbiotic organelles are crucial to survival and provide well-validated and widely utilised drug targets in parasitic protists such as Plasmodium and Toxoplasma. However, mutations within the organellar genomes of mitochondria and plastids can lead to drug resistance. Such mutations ultimately challenge our ability to control and eradicate the diseases caused b

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Havemann, Gregory D., and Thomas A. Bobik. "Protein Content of Polyhedral Organelles Involved in Coenzyme B12-Dependent Degradation of 1,2-Propanediol in Salmonella enterica Serovar Typhimurium LT2." Journal of Bacteriology 185, no. 17 (2003): 5086–95. http://dx.doi.org/10.1128/jb.185.17.5086-5095.2003.

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ABSTRACT Salmonella enterica forms polyhedral organelles during coenzyme B12-dependent growth on 1,2-propanediol (1,2-PD). Previously, these organelles were shown to consist of a protein shell partly composed of the PduA protein, the majority of the cell's B12-dependent diol dehydratase, and additional unidentified proteins. In this report, the polyhedral organelles involved in B12-dependent 1,2-PD degradation by S. enterica were purified by a combination of detergent extraction and differential and density gradient centrifugation. The course of the purification was monitored by electron micro

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Marks, Michael S., Harry FG Heijnen, and Graça Raposo. "Lysosome-related organelles: unusual compartments become mainstream." Current Opinion in Cell Biology 25, no. 4 (2013): 495–505. http://dx.doi.org/10.1016/j.ceb.2013.04.008.

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Yang, Rui, Tao Zhu, Jingyang Xu, et al. "Organic Fluorescent Probes for Monitoring Micro-Environments in Living Cells and Tissues." Molecules 28, no. 8 (2023): 3455. http://dx.doi.org/10.3390/molecules28083455.

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As a vital parameter in living cells and tissues, the micro-environment is crucial for the living organisms. Significantly, organelles require proper micro-environment to achieve normal physiological processes, and the micro-environment in organelles can reflect the state of organelles in living cells. Moreover, some abnormal micro-environments in organelles are closely related to organelle dysfunction and disease development. So, visualizing and monitoring the variation of micro-environments in organelles is helpful for physiologists and pathologists to study the mechanisms of the relative di

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Lee, Dongun, and Jeong Hee Hong. "Niemann-Pick Disease Type C (NPDC) by Mutation of NPC1 and NPC2: Aberrant Lysosomal Cholesterol Trafficking and Oxidative Stress." Antioxidants 12, no. 12 (2023): 2021. http://dx.doi.org/10.3390/antiox12122021.

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Cholesterol trafficking is initiated by the endocytic pathway and transported from endo/lysosomes to other intracellular organelles. Deficiencies in cholesterol-sensing and binding proteins NPC1 and NPC2 induce accumulation in lysosomes and the malfunction of trafficking to other organelles. Each organelle possesses regulatory factors to induce cholesterol trafficking. The mutation of NPC1 and NPC2 genes induces Niemann-Pick disease type C (NPDC), which is a hereditary disease and causes progressive neurodegeneration, developmental disability, hypotonia, and ataxia. Oxidative stress induces da

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BRENT HEATH, I., and SUSAN G.W. KAMINSKYJ. "The organization of tip-growth-related organelles and microtubules revealed by quantitative analysis of freeze-substituted oomycete hyphae." Journal of Cell Science 93, no. 1 (1989): 41–52. http://dx.doi.org/10.1242/jcs.93.1.41.

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The distribution of organelles and microtubules in hyphal tips of the oomycete, Saprolegnia ferax, were quantitatively determined at high resolution from serial-section electron microscopy of freeze-substituted cells. All the organelles and the microtubules were non-uniformly distributed, each showing a characteristic longitudinal gradient starting at a different point behind the tip. In addition, when the cytoplasmic cross-sectional area was divided into radial regions, all organelles occurred preferentially in either the central (mitochondria and Golgi bodies) or the peripheral (microtubules

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Audano, Matteo, Silvia Pedretti, Simona Ligorio, et al. "“The Loss of Golden Touch”: Mitochondria-Organelle Interactions, Metabolism, and Cancer." Cells 9, no. 11 (2020): 2519. http://dx.doi.org/10.3390/cells9112519.

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Mitochondria represent the energy hub of cells and their function is under the constant influence of their tethering with other subcellular organelles. Mitochondria interact with the endoplasmic reticulum, lysosomes, cytoskeleton, peroxisomes, and nucleus in several ways, ranging from signal transduction, vesicle transport, and membrane contact sites, to regulate energy metabolism, biosynthetic processes, apoptosis, and cell turnover. Tumorigenesis is often associated with mitochondrial dysfunction, which could likely be the result of an altered interaction with different cell organelles or st

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MARGOS, G., L. H. BANNISTER, A. R. DLUZEWSKI, J. HOPKINS, I. T. WILLIAMS, and G. H. MITCHELL. "Correlation of structural development and differential expression of invasion-related molecules in schizonts ofPlasmodium falciparum." Parasitology 129, no. 3 (2004): 273–87. http://dx.doi.org/10.1017/s0031182004005657.

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During asexual developmentPlasmodiumschizonts undergo a series of complex biochemical and structural changes. Using tightly synchronized cultures of 2P. falciparumlines (clone C10 and strain ITO4) for light microscopy and fluorescence imaging we monitored the timing and sequence of expression of proteins associated with invasion-related organelles. Antibodies to rhoptry, micronemal and dense granule proteins (Rhoptry Associated Protein 1, Apical Membrane Antigen 1, Erythrocyte Binding Antigen 175, Ring-infected Erythrocyte Surface Antigen) and to pellicle-associated proteins (Merozoite Surface

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Wang, Jian, Long Bai, Yingying Jing, and Jiacan Su. "Organelles and Extracellular Vesicles in Bone Aging." Organelle 3 (June 5, 2025): 3. https://doi.org/10.61747/0ifp.202503003.

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Bone aging is characterized by a decline in bone mass and structural integrity, increasing fracture and osteoporosis risks in the elderly. This process involves cellular and molecular changes, including impaired osteoblast and osteoclast activity, reduced osteocyte viability, and disrupted intercellular communication within the bone microenvironment. Organelles such as mitochondria, endoplasmic reticulum (ER), lysosomes, and the Golgi apparatus play crucial roles in maintaining cellular homeostasis. During aging, mitochondria show reduced ATP production and increased reactive oxygen species, l

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JOHNSON, L., J. S. GRUMBLES, S. CHASTAIN, H. F. GOSS, and C. S. PETTY. "Leydig Cell Cytoplasmic Content Is Related to Daily Sperm Production in Men." Journal of Andrology 11, no. 2 (1990): 155–60. http://dx.doi.org/10.1002/j.1939-4640.1990.tb01595.x.

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The relationship between the abundance of specific Leydig cell organelles and daily sperm production (DSP) was determined. Testes from 10 men (26–53 years of age) were obtained at autopsy within 10 h of traumatic death or heart failure and fixed by vascular perfusion. Testicular tissue was processed for light and electron microscopy. DSP/testis and Leydig cell cytoplasmic volume/testis were determined by stereology of histologic sections. The Leydig cell organelle content was determined by point counting electron micrographs for smooth endoplasmic reticulum (SER), rough endoplasmic reticulum,

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Scott, Iain, and Richard J. Youle. "Mitochondrial fission and fusion." Essays in Biochemistry 47 (June 14, 2010): 85–98. http://dx.doi.org/10.1042/bse0470085.

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Mitochondria are highly dynamic cellular organelles, with the ability to change size, shape and position over the course of a few seconds. Many of these changes are related to the ability of mitochondria to undergo the highly co-ordinated processes of fission (division of a single organelle into two or more independent structures) or fusion (the opposing reaction). These actions occur simultaneously and continuously in many cell types, and the balance between them regulates the overall morphology of mitochondria within any given cell. Fission and fusion are active processes which require many

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Watts, Colin. "Lysosomes and lysosome‐related organelles in immune responses." FEBS Open Bio 12, no. 4 (2022): 678–93. http://dx.doi.org/10.1002/2211-5463.13388.

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Luzio, J. P., Y. Hackmann, N. M. G. Dieckmann, and G. M. Griffiths. "The Biogenesis of Lysosomes and Lysosome-Related Organelles." Cold Spring Harbor Perspectives in Biology 6, no. 9 (2014): a016840. http://dx.doi.org/10.1101/cshperspect.a016840.

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Blaby-Haas, Crysten E., and Sabeeha S. Merchant. "Lysosome-related Organelles as Mediators of Metal Homeostasis." Journal of Biological Chemistry 289, no. 41 (2014): 28129–36. http://dx.doi.org/10.1074/jbc.r114.592618.

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Osanai, Kazuhiro. "Rab38 Mutation and the Lung Phenotype." International Journal of Molecular Sciences 19, no. 8 (2018): 2203. http://dx.doi.org/10.3390/ijms19082203.

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Rab38 is highly expressed in alveolar type II cells, melanocytes, and platelets. These cells are specifically-differentiated cells and contain characteristic intracellular organelles called lysosome-related organelles, i.e., lamellar bodies in alveolar type II cells, melanosomes in melanocytes, and dense granules in platelets. There are Rab38-mutant rodents, i.e., chocolate mice and Ruby rats. While chocolate mice only show oculocutaneous albinism, Ruby rats show oculocutaneous albinism and prolonged bleeding time and, hence, are a rat model of Hermansky-Pudlak syndrome (HPS). Most patients wi

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Ellis, Kathryn, Jennifer Bagwell, and Michel Bagnat. "Notochord vacuoles are lysosome-related organelles that function in axis and spine morphogenesis." Journal of Cell Biology 200, no. 5 (2013): 667–79. http://dx.doi.org/10.1083/jcb.201212095.

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The notochord plays critical structural and signaling roles during vertebrate development. At the center of the vertebrate notochord is a large fluid-filled organelle, the notochord vacuole. Although these highly conserved intracellular structures have been described for decades, little is known about the molecular mechanisms involved in their biogenesis and maintenance. Here we show that zebrafish notochord vacuoles are specialized lysosome-related organelles whose formation and maintenance requires late endosomal trafficking regulated by the vacuole-specific Rab32a and H+-ATPase–dependent ac

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Raimundo, Nuno. "IMPACT OF MITOCHONDRIAL MALFUNCTION IN AGING IS MEDIATED BY IMPAIRED LYSOSOMES AND ENDOPLASMIC RETICULUM." Innovation in Aging 7, Supplement_1 (2023): 592. http://dx.doi.org/10.1093/geroni/igad104.1938.

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Abstract The hallmarks of aging are well established, and include mitochondrial malfunction, deregulated nutrient sensing, loss of proteostasis, and senescence, among others. While these hallmarks are all part of the aging process, they have typically been studied independently. We have found that mitochondrial malfunction can affect lysosomal activity, impacting nutrient sensing, and our that the crosstalk between mitochondria and other organelles such as endoplasmic reticulum and lysosomes affects cellular senescence, proteostasis and intercellular communication. Chronic mitochondrial malfun

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Yoo, Byung-Chun, Narendra S. Yadav, Emil M. Orozco, and Hajime Sakai. "Cas9/gRNA-mediated genome editing of yeast mitochondria and Chlamydomonas chloroplasts." PeerJ 8 (January 6, 2020): e8362. http://dx.doi.org/10.7717/peerj.8362.

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We present a new approach to edit both mitochondrial and chloroplast genomes. Organelles have been considered off-limits to CRISPR due to their impermeability to most RNA and DNA. This has prevented applications of Cas9/gRNA-mediated genome editing in organelles while the tool has been widely used for engineering of nuclear DNA in a number of organisms in the last several years. To overcome the hurdle, we designed a new approach to enable organelle genome editing. The plasmids, designated “Edit Plasmids,” were constructed with two expression cassettes, one for the expression of Cas9, codon-opt

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León-Avila, Gloria, and Jorge Tovar. "Mitosomes of Entamoeba histolytica are abundant mitochondrion-related remnant organelles that lack a detectable organellar genome." Microbiology 150, no. 5 (2004): 1245–50. http://dx.doi.org/10.1099/mic.0.26923-0.

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The existence of mitochondrion-related relict organelles (mitosomes) in the amitochondrial human pathogen Entamoeba histolytica and the detection of extranuclear DNA-containing cytoplasmic structures (EhKOs) has led to the suggestion that a remnant genome from the original mitochondrial endosymbiont might have been retained in this organism. This study reports on the mutually exclusive distribution of Cpn60 and extranuclear DNA in E. histolytica and on the distribution of Cpn60-containing mitosomes in this parasite. In situ nick-translation coupled to immunofluorescence microscopy failed to de

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Chen, Bochao, Haotong Yuan, Wei Zhang, Jingjing Hu, Xiaoding Lou, and Fan Xia. "AIEgen-Peptide Bioprobes for the Imaging of Organelles." Biosensors 12, no. 8 (2022): 667. http://dx.doi.org/10.3390/bios12080667.

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Organelles are important subsystems of cells. The damage and inactivation of organelles are closely related to the occurrence of diseases. Organelles’ functional activity can be observed by fluorescence molecular tools. Nowadays, a series of aggregation-induced emission (AIE) bioprobes with organelles-targeting ability have emerged, showing great potential in visualizing the interactions between probes and different organelles. Among them, AIE luminogen (AIEgen)-based peptide bioprobes have attracted more and more attention from researchers due to their good biocompatibility and photostability

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Gaffke, Lidia, Karolina Pierzynowska, Estera Rintz, Zuzanna Cyske, Izabela Giecewicz, and Grzegorz Węgrzyn. "Gene Expression-Related Changes in Morphologies of Organelles and Cellular Component Organization in Mucopolysaccharidoses." International Journal of Molecular Sciences 22, no. 5 (2021): 2766. http://dx.doi.org/10.3390/ijms22052766.

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Mucopolysaccharidoses (MPS) are inherited metabolic diseases characterized by accumulation of incompletely degraded glycosaminoglycans (GAGs) in lysosomes. Although primary causes of these diseases are mutations in genes coding for enzymes involved in lysosomal GAG degradation, it was demonstrated that storage of these complex carbohydrates provokes a cascade of secondary and tertiary changes affecting cellular functions. Potentially, this might lead to appearance of cellular disorders which could not be corrected even if the primary cause of the disease is removed. In this work, we studied ch

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Aung, Kyaw, Xinchun Zhang, and Jianping Hu. "Peroxisome division and proliferation in plants." Biochemical Society Transactions 38, no. 3 (2010): 817–22. http://dx.doi.org/10.1042/bst0380817.

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Peroxisomes are eukaryotic organelles with crucial functions in development. Plant peroxisomes participate in various metabolic processes, some of which are co-operated by peroxisomes and other organelles, such as mitochondria and chloroplasts. Defining the complete picture of how these essential organelles divide and proliferate will be instrumental in understanding how the dynamics of peroxisome abundance contribute to changes in plant physiology and development. Research in Arabidopsis thaliana has identified several evolutionarily conserved major components of the peroxisome division machi

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Koch, Johannes, and Cécile Brocard. "Membrane elongation factors in organelle maintenance: the case of peroxisome proliferation." BioMolecular Concepts 2, no. 5 (2011): 353–64. http://dx.doi.org/10.1515/bmc.2011.031.

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AbstractSeparation of metabolic pathways in organelles is critical for eukaryotic life. Accordingly, the number, morphology and function of organelles have to be maintained through processes linked with membrane remodeling events. Despite their acknowledged significance and intense study many questions remain about the molecular mechanisms by which organellar membranes proliferate. Here, using the example of peroxisome proliferation, we give an overview of how proteins elongate membranes. Subsequent membrane fission is achieved by dynamin-related proteins shared with mitochondria. We discuss b

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Yeates, Todd O., Cheryl A. Kerfeld, Sabine Heinhorst, Gordon C. Cannon, and Jessup M. Shively. "Protein-based organelles in bacteria: carboxysomes and related microcompartments." Nature Reviews Microbiology 6, no. 9 (2008): 681–91. http://dx.doi.org/10.1038/nrmicro1913.

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Raposo, Graça, Benoit Fevrier, Willem Stoorvogel, and Michael S. Marks. "Lysosome-Related Organelles: a View from Immunity and Pigmentation." Cell Structure and Function 27, no. 6 (2002): 443–56. http://dx.doi.org/10.1247/csf.27.443.

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Raposo, Graça, Michael S. Marks, and Daniel F. Cutler. "Lysosome-related organelles: driving post-Golgi compartments into specialisation." Current Opinion in Cell Biology 19, no. 4 (2007): 394–401. http://dx.doi.org/10.1016/j.ceb.2007.05.001.

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Sant’Anna, Celso, Fabiola Parussini, Daniela Lourenço, Wanderley Souza, Juan Jose Cazzulo, and Narcisa Leal Cunha-e-Silva. "All Trypanosoma cruzi developmental forms present lysosome-related organelles." Histochemistry and Cell Biology 130, no. 6 (2008): 1187–98. http://dx.doi.org/10.1007/s00418-008-0486-8.

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Makiuchi, Takashi, and Tomoyoshi Nozaki. "Highly divergent mitochondrion-related organelles in anaerobic parasitic protozoa." Biochimie 100 (May 2014): 3–17. http://dx.doi.org/10.1016/j.biochi.2013.11.018.

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Dyall, Sabrina D., Carla M. Koehler, Maria G. Delgadillo-Correa, et al. "Presence of a Member of the Mitochondrial Carrier Family in Hydrogenosomes: Conservation of Membrane-Targeting Pathways between Hydrogenosomes and Mitochondria." Molecular and Cellular Biology 20, no. 7 (2000): 2488–97. http://dx.doi.org/10.1128/mcb.20.7.2488-2497.2000.

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ABSTRACT A number of microaerophilic eukaryotes lack mitochondria but possess another organelle involved in energy metabolism, the hydrogenosome. Limited phylogenetic analyses of nuclear genes support a common origin for these two organelles. We have identified a protein of the mitochondrial carrier family in the hydrogenosome ofTrichomonas vaginalis and have shown that this protein, Hmp31, is phylogenetically related to the mitochondrial ADP-ATP carrier (AAC). We demonstrate that the hydrogenosomal AAC can be targeted to the inner membrane of mitochondria isolated from Saccharomyces cerevisia

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Zhang, Guang, Chunping Jiang, Zhongxia Wang, Weibo Chen, Wen Gu, and Yitao Ding. "Dehydroabietic Acid Derivative QC2 Induces Oncosis in Hepatocellular Carcinoma Cells." BioMed Research International 2014 (2014): 1–11. http://dx.doi.org/10.1155/2014/682197.

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Aim.Rosin, the traditional Chinese medicine, is reported to be able to inhibit skin cancer cell lines. In this report, we investigate the inhibitory effect against HCC cells of QC2, the derivative of rosin’s main components dehydroabietic acid.Methods.MTT assay was used to determine the cytotoxicity of QC2. Morphological changes were observed by time-lapse microscopy and transmission electron microscopy and the cytoskeleton changes were observed by laser-scanning confocal microscopy. Cytomembrane integrity and organelles damage were confirmed by detection of the reactive oxygen (ROS), lactate

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