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Artykuły w czasopismach na temat „RFLP”

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1

Trevisan, Giovani, Aditi Sharma, Phillip Gauger, et al. "PRRSV2 genetic diversity defined by RFLP patterns in the United States from 2007 to 2019." Journal of Veterinary Diagnostic Investigation 33, no. 5 (2021): 920–31. http://dx.doi.org/10.1177/10406387211027221.

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The genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) increases over time. In 1998, restriction-fragment length polymorphism (RFLP) pattern analysis was introduced to differentiate PRRSV wild-type strains from VR2332, a reference strain from which a commercial vaccine (Ingelvac PRRS MLV) was derived. We have characterized here the PRRSV genetic diversity within selected RFLP families over time and U.S. geographic space, using available ISU-VDL data from 2007 to 2019. The 40,454 ORF5 sequences recovered corresponded to 228 distinct RFLPs. Four RFLPs [2-5-2 (21.2%)

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2

Young, Nevin Dale. "Restriction Fragment Length Polymorphisms (RFLPS) and Crop Improvement." Experimental Agriculture 28, no. 4 (1992): 385–98. http://dx.doi.org/10.1017/s0014479700020093.

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SUMMARYRestriction fragment length polymorphisms (RFLPs) are genetic markers based on cloned molecules of DNA. Using RFLPs, scientists can construct high density genetic linkage maps and locate economically important genes. Once a gene for a valuable trait has been mapped with RFLPs, desirable genotypes can be selected using RFLPs rather than scoring for the trait itself. This is important when a trait is recessive, difficult to score, or obscured by other characters. RFLPs are particularly valuable in mapping genes controlling complex polygenic characters, including those fundamental to crop

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Meagher, R. B., M. D. McLean, and J. Arnold. "Recombination within a subclass of restriction fragment length polymorphisms may help link classical and molecular genetics." Genetics 120, no. 3 (1988): 809–18. http://dx.doi.org/10.1093/genetics/120.3.809.

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Abstract Restriction fragment length polymorphisms (RFLPs) are being used to construct complete linkage maps for many eukaryotic genomes. These RFLP maps can be used to predict the inheritance of important phenotypic loci and will assist in the molecular cloning of linked gene(s) which affect phenotypes of scientific, medical and agronomic importance. However, genetic linkage implies very little about the actual physical distances between loci. An assay is described which uses genetic recombinants to measure physical distance from a DNA probe to linked phenotypic loci. We have defined the subs

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4

Gedil, Melaku Ayele, Crispin Wye, Simon Berry, et al. "An integrated restriction fragment length polymorphism - amplified fragment length polymorphism linkage map for cultivated sunflower." Genome 44, no. 2 (2001): 213–21. http://dx.doi.org/10.1139/g00-111.

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Restriction fragment length polymorphism (RFLP) maps have been constructed for cultivated sunflower (Helianthus annuus L.) using three independent sets of RFLP probes. The aim of this research was to integrate RFLP markers from two sets with RFLP markers for resistance gene candidate (RGC) and amplified fragment length polymorphism (AFLP) markers. Genomic DNA samples of HA370 and HA372, the parents of the F2 population used to build the map, were screened for AFLPs using 42 primer combinations and RFLPs using 136 cDNA probes (RFLP analyses were performed on DNA digested with EcoRI, HindIII, Ec

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5

Deynze, A. E. Van, B. S. Landry, and K. P. Pauls. "The identification of restriction fragment length polymorphisms linked to seed colour genes in Brassica napus." Genome 38, no. 3 (1995): 534–42. http://dx.doi.org/10.1139/g95-069.

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Restriction fragment length polymorphisms (RFLPs) linked to genes controlling seed colour were identified in rapeseed (Brassica napus). The efficiency of the RFLP analysis was enhanced by utilizing bulked segregant analysis, DNA clones that had previously been used to construct a RFLP map of B. napus, and a doubled-haploid (DH) population segregating for seed colour. Markers for two of the three seed colour genes segregating in the DH population were identified on the basis of χ2 analyses of marker distributions among visually classified black-, brown-, and yellow-seeded DH lines as well as AN

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6

Seldin, M. F., H. C. Morse, J. P. Reeves, C. L. Scribner, R. C. LeBoeuf, and A. D. Steinberg. "Genetic analysis of autoimmune gld mice. I. Identification of a restriction fragment length polymorphism closely linked to the gld mutation within a conserved linkage group." Journal of Experimental Medicine 167, no. 2 (1988): 688–93. http://dx.doi.org/10.1084/jem.167.2.688.

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A linkage map of distal mouse chromosome 1 was generated using restriction fragment length polymorphism (RFLP) analysis of DNA prepared from 95 [C3H-gld/gld X Mus spretus)F1 X C3H-gld/gld] backcross mice. The gene order was: (centromere) C4bp, Ren-1,2, Ly-5, [At-3/gld], Apoa-2/Ly-17, Spna-1 (telomere). All mice expressing the phenotype of gld homozygotes were homozygous for the At-3 RFLP characteristic of C3H mice and none of the mice heterozygous for At-3 RFLPs had characteristics of gld homozygotes, demonstrating close linkage between these genes. The identification of an RFLP closely linked

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7

Jena, K. K., G. S. Khush, and G. Kochert. "Comparative RFLP mapping of a wild rice, Oryza officinalis, and cultivated rice, O. sativa." Genome 37, no. 3 (1994): 382–89. http://dx.doi.org/10.1139/g94-054.

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A comparative RFLP map was constructed in a wild rice, Oryza officinalis, by using 139 genomic and cDNA probes that had been used previously to map RFLPs in O. sativa. Nine of the 12 chromosomes of O. officinalis were highly homosequential to those of O. sativa. A major rearrangement of gene order was detected in chromosome 1 and small inversions were found in chromosomes 3 and 11. Fourteen translocated RFLP markers were found, and chromosome 11 contained a high frequency of such translocated segments. Results were consistent with meiotic and trisomie analysis, which suggested that the genomes

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8

Landry, Benoit S., Nathalie Hubert, René Crete, Morgan S. Chang, Steven E. Lincoln, and Takeomi Etoh. "A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin)." Genome 35, no. 3 (1992): 409–20. http://dx.doi.org/10.1139/g92-061.

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F2 segregation analyses of DNA restriction fragment length polymorphisms (RFLPs) detected between a cabbage line (No. 86-16-5) resistant to race 2 of Plasmodiophora brassicae (Woronin), the fungus responsible for clubroot disease, and a rapid cycling line (CrGC No. 85) was used to construct a detailed genetic map of Brassica oleracea. RFLP markers were random and seedling-specific cDNA clones. The 201 loci so far mapped in B. oleracea covered 1112 cM. They are assembled into nine major linkage groups and four small linkage groups. Twelve loci were found unlinked to any other markers. Twenty-on

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9

Garcia, G. M., H. T. Stalker, and G. Kochert. "Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers." Genome 38, no. 1 (1995): 166–76. http://dx.doi.org/10.1139/g95-021.

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Forty-six introgression lines (F10C9) from a cross between Arachis hypogaea L. (2n = 4x = 40) and A. cardenasii Krapov. &W.C. Gregory (2n = 2x = 20) were analyzed for the introgression of A. cardenasii chromosome segments. Seventy-three RFLP probes and 70 RAPD primers, expressing from one to four A. cardenasii-specific bands, were used to evaluate the set of introgression lines. Thirty-four RFLP probes and 45 RAPD primers identified putative A. cardenasii introgressed chromosome segments in one or more lines. Introgressed segments were detected by RFLP analysis in 10 of the 11 linkage grou

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10

King, Joseph J., James M. Bradeen, and Michael J. Havey. "Variability for Restriction Fragment-length Polymorphisms (RFLPs) and Relationships among Elite Commercial Inbred and Virtual Hybrid Onion Populations." Journal of the American Society for Horticultural Science 123, no. 6 (1998): 1034–37. http://dx.doi.org/10.21273/jashs.123.6.1034.

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Nuclear RFLPs were used to estimate relationships among 14 elite commercial inbreds of bulb onion (Allium cepa) from Holland, Japan, and the United States. Variability for known alleles at 75 RFLP loci and 194 polymorphic fragments revealed by 69 anonymous cDNA probes and a clone of alliinase were scored to yield genetically characterized and uncharacterized data sets, respectively. The inbred onion populations possessed more than two alleles at 20 of 43 (46%) codominant RFLP loci. Relationships among the inbreds were estimated by cluster analysis of simple-matching (genetically characterized

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11

Kennard, Wayne, Arian Dijkhuizen, Michael Havey, and Jack Staub. "PROGRESS TOWARD DEVELOPMENT OF AN RFLP MAP FOR CUCUMBER." HortScience 25, no. 9 (1990): 1159a—1159. http://dx.doi.org/10.21273/hortsci.25.9.1159a.

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The analysis of genetic linkage in cucumber (Cucumis sativus has primarily involved morphological and disease resistance markers. Linkage analysis in cucumber would benefit from more markers. Restriction fragment length polymorphisms (RFLPs) can occur in relatively large numbers within a single segregating family. Research is presently underway to construct an RFLP map of cucumber. Pst I partial genomic and cDNA libraries of cucumber have been constructed as sources of probes for RFLP analysis. Cucumber DNA from 16 accessions of cucumber and one accession of C. sativus var. hardwickii were dig

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12

Echt, C. S., K. K. Kidwell, S. J. Knapp, T. C. Osborn, and T. J. McCoy. "Linkage mapping in diploid alfalfa (Medicago sativa)." Genome 37, no. 1 (1994): 61–71. http://dx.doi.org/10.1139/g94-008.

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A genome map of cultivated alfalfa was constructed using segregating restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNAs (RAPDs) in a diploid backcross population generated from noninbred parents. Among the 153 loci scored in 87 progeny, four segregation ratios were observed for codominant and dominant markers: 1:1, 1:2:1, 1:1:1:1, and 3:1. Deviations from expected Mendelian ratios (p < 0.05) were observed for 34% of the loci studied. A genome map was assembled from two separate linkage maps, each constructed from a subset of the segregation data. One lin

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13

Givney, Rodney, Alison Vickery, Anne Holliday, Mary Pegler, and Richard Benn. "Evolution of an Endemic Methicillin-ResistantStaphylococcus aureus Population in an Australian Hospital from 1967 to 1996." Journal of Clinical Microbiology 36, no. 2 (1998): 552–56. http://dx.doi.org/10.1128/jcm.36.2.552-556.1998.

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The evolution over 30 years of a population of methicillin-resistant Staphylococcus aureus (MRSA) from a tertiary referral hospital was studied by phylogenetic analysis ofSmaI-generated restriction fragment length polymorphisms (RFLPs). The results suggest that a new clone of MRSA appeared at the hospital in the early 1980s, which, although usually retaining its ancestral phage-type, developed four different RFLP pulsotypes in the next 16 years. This finding indicates that multiple RFLP patterns in MRSA do not necessarily represent multiple clones deriving from different mec gene transfer even

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14

Hulbert, S. H., T. W. Ilott, E. J. Legg, S. E. Lincoln, E. S. Lander, and R. W. Michelmore. "Genetic analysis of the fungus, Bremia lactucae, using restriction fragment length polymorphisms." Genetics 120, no. 4 (1988): 947–58. http://dx.doi.org/10.1093/genetics/120.4.947.

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Abstract Restriction fragment length polymorphisms (RFLPs) were developed as genetic markers for Bremia lactucae, the biotrophic Oomycete fungus which causes lettuce downy mildew. By using 55 genomic and cDNA probes, a total of 61 RFLP loci were identified among three heterothallic isolates of B. lactucae. Of these 61 RFLP loci, 53 were heterozygous in at least one of the three strains and thus were informative for linkage analysis in at least one of two F1 crosses that were performed. Analysis of the cosegregation of these 53 RFLPs, eight avirulence loci and the mating type locus allowed the

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15

Landry, Benoit S., Nathalie Hubert, Takeomi Etoh, John J. Harada, and Stephen E. Lincoln. "A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences." Genome 34, no. 4 (1991): 543–52. http://dx.doi.org/10.1139/g91-084.

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F2 segregation analyses of DNA restriction fragment length polymorphisms (RFLPs) detected between two cultivars of canola ('Westar' × Topas') was used to construct a detailed genetic map of Brassica napus. RFLP markers were from a seedling-specific cDNA library. They were either randomly selected or previously characterized as seedling-specific cDNA clones. The 120 loci so far mapped in B. napus covered 1413 recombination units. They are assembled into 19 linkage groups. Seventeen loci were found unlinked to any other markers. Few polymorphisms were detected with the seedling-specific cDNAs an

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16

Galgaro, Leticia, Catalina Romero Lopes, Marcos Gimenes, José FM Valls, and Gary Kochert. "Genetic variation between several species of sections Extranervosae, Caulorrhizae, Heteranthae, and Triseminatae (genus Arachis) estimated by DNA polymorphism." Genome 41, no. 3 (1998): 445–54. http://dx.doi.org/10.1139/g98-004.

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Genetic variation within and among accessions of the genusArachis representing sections Extranervosae, Caulorrhizae, Heteranthae, and Triseminatae was evaluated using RFLP and RAPD markers. RAPD markers revealed a higher level of genetic diversity than did RFLP markers, both within and among the species evaluated. Phenograms based on various band-matching algorithms revealed three major clusters of similarity among the sections evaluated. The first group included the species from section Extranervosae, the second group consisted of sections Triseminatae, Caulorrhizae, and Heteranthae, and the

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Griffiths, H. M., W. A. Sinclair, E. Boudon-Padieu, et al. "Phytoplasmas Associated with Elm Yellows: Molecular Variability and Differentiation from Related Organisms." Plant Disease 83, no. 12 (1999): 1101–4. http://dx.doi.org/10.1094/pdis.1999.83.12.1101.

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Restriction fragment length polymorphism (RFLP) analyses were performed on polymerase chain reaction (PCR) amplimers of phytoplasmal DNA from eight samples obtained from Ulmus spp. (elms) affected by elm yellows (EY) in Italy and the United States, from Catharanthus roseus infected with strain EY1, and from five other plant species infected with phytoplasmas of the EY group sensu lato (group 16SrV). RFLP profiles obtained with restriction enzyme TaqI from ribosomal DNA amplified with primer pair P1/P7 differentiated elm-associated phytoplasmas from strains originally detected in Apocynum canna

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Kuspa, A., and W. F. Loomis. "REMI-RFLP mapping in the Dictyostelium genome." Genetics 138, no. 3 (1994): 665–74. http://dx.doi.org/10.1093/genetics/138.3.665.

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Abstract A set of 147 Dictyostelium discoideum strains was constructed by random integration of a vector containing rare restriction sites. The strains were generated by transformation using restriction enzyme-mediated integration (REMI) which results in the integration of linear DNA fragments into randomly distributed genomic restriction sites. Restriction fragment length polymorphism (RFLP) was generated in a single genomic site in each strain. These REMI-RFLP strains were used to confirm gene linkages previously supported by two other physical mapping techniques: yeast artificial chromosome

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IQBAL, S. J., D. S. PLAHA, G. H. LINFORTH, and R. DALGLEISH. "Hypophosphatasia: diagnostic application of linked DNA markers in the dominantly inherited adult form." Clinical Science 97, no. 1 (1999): 73–78. http://dx.doi.org/10.1042/cs0970073.

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Hypophosphatasia is a rare disease characterized by low serum levels of tissue non-specific alkaline phosphatase (TNSALP) and a spectrum of skeletal disease varying from the severest form with death in utero to mild with no clinical abnormality in adults. Currently, the diagnosis of hypophosphatasia is made on the basis of clinical findings, radiography, low serum alkaline phosphatase levels and raised abnormal phosphorylated metabolites; there are elevations in serum pyridoxal 5′-phosphate, urinary phosphoethanolamine and inorganic pyrophosphate. In borderline cases the biochemical diagnosis

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Jordan, D. R., R. E. Casu, P. Besse, B. C. Carroll, N. Berding, and C. L. McIntyre. "Markers associated with stalk number and suckering in sugarcane colocate with tillering and rhizomatousness QTLs in sorghum." Genome 47, no. 5 (2004): 988–93. http://dx.doi.org/10.1139/g04-040.

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Two important factors influencing sugar yield, the primary focus of sugarcane plant breeding programs, are stalk number and suckering. Molecular markers linked to both of these traits are sought to assist in the identification of high sugar yield, high stalk number, low-suckering sugarcane clones. In this preliminary mapping study, 108 progeny from a biparental cross involving two elite Australian sugarcane clones were evaluated at two sites for two years for both stalk number and suckering. A total of 258 DNA markers, including both restriction fragment length polymorphisms (RFLPs) and radio-

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21

Whisson, SC, BJ Howlett, ECY Liew, DJ Maclean, JM Manners, and JAG Irwin. "An Assessment of Genetic Relationships between Members of the Phytophthora megasperma Complex and Phytophthora vignae using Molecular Markers." Australian Systematic Botany 6, no. 4 (1993): 295. http://dx.doi.org/10.1071/sb9930295.

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Genetic relationships between Phytophora megasperma f. sp. glycinea (Pmg) and morphologically similar taxa, P. megasperma f. sp. medicaginis (Pmm), P. megasperma f. sp. trifolii (Pmt), P. megasperma from Douglas Fir (PmDF) and asparagus (PmAS) and Phytophthora vignae, were explored by restriction fragment length polymorphism (RFLP) analysis of nuclear DNA using random genomic multi-copy, cDNA, and ribosomal DNA probes as well as random amplified polymorphic DNA (RAPDs) and RFLP analysis of ribosomal intergenic spacer regions amplified by the polymerase chain reaction (PCR). Each method detecte

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Zerba, K. E., A. M. Kessling, J. Davignon, and C. F. Sing. "Genetic structure and the search for genotype-phenotype relationships: an example from disequilibrium in the Apo B gene region." Genetics 129, no. 2 (1991): 525–33. http://dx.doi.org/10.1093/genetics/129.2.525.

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Abstract We analyzed allelic associations (disequilibria) for four restriction fragment length polymorphisms (RFLPs) in the region of the 43-kb Apo B gene in a sample of 233 unrelated individuals from Montreal, Canada, sampled for health. This total sample (T) included 160 individuals of known French Canadian (FC) ancestry. We present a rigorous application of current methodology to these samples, including estimation of type II error probabilities and correlations between markers for estimates of disequilibria. We then consider the utility of these estimates of allelic disequilibria for the i

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Liou, Pan-chi, Fred G. Gmitter, and Gloria A. Moore. "MOLECULAR CHARACTERIZATION AND LINKAGE MAPPING OF THE CITRUS GENOME USING ISOZYME AND RFLP MARKERS." HortScience 25, no. 9 (1990): 1154c—1154. http://dx.doi.org/10.21273/hortsci.25.9.1154c.

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Citrus genetic studies and cultivar improvement have been difficult with conventional techniques. Alternative approaches are needed to enhance efficiency of such studies. Our objectives were to characterize the Citrus genome and to initiate development of a linkage map using RFLP and isozyme analysis. Methods of Citrus DNA extraction were developed to allow the isolation of chromosomal DNA of acceptable quality for recombinant' DNA manipulations. A PstI Citrus genomic library was constructed to create DNA clones for the RFLP survey. A rapid, reliable procedure was developed to facilitate scree

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Keller, S. M., J. M. McDermott, R. E. Pettway, M. S. Wolfe, and B. A. McDonald. "Gene Flow and Sexual Reproduction in the Wheat Glume Blotch Pathogen Phaeosphaeria nodorum (Anamorph Stagonospora nodorum)." Phytopathology® 87, no. 3 (1997): 353–58. http://dx.doi.org/10.1094/phyto.1997.87.3.353.

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Restriction fragment length polymorphisms (RFLPs) were used to characterize the genetic structures of three field populations of Phaeosphaeria nodorum from Texas, Oregon, and Switzerland. Data from seven nuclear RFLP loci were used to estimate gene diversity and genetic distances and to make indirect measures of gene flow between populations. Three of the seven RFLP loci differed significantly in allele frequencies across populations. On average, 96% of the total gene diversity was found within populations. There was little evidence for population subdivision, suggesting that gene flow was not

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Gedeon, A. K., J. C. Mulley, and A. Fratini. "AnMsp1 RFLP atD16S10." Nucleic Acids Research 17, no. 5 (1989): 2146. http://dx.doi.org/10.1093/nar/17.5.2146.

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Patterson, N. A., and M. Kapoor. "Detection of additional restriction fragment length polymorphisms among the weakly virulent (nonaggressive) and highly virulent (aggressive) isolates of Leptosphaeria maculans." Canadian Journal of Microbiology 41, no. 12 (1995): 1135–41. http://dx.doi.org/10.1139/m95-158.

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Isolates of Leptosphaeria maculans were analyzed for their genetic relatedness based on DNA restriction fragment length polymorphisms (RFLPs), employing as Southern hybridization probes a combination of heat shock responsive genes (hsp70 and hsp80 from Neurospora crassa), the cutinase gene of Magnaporthe grisea, and cloned genomic DNA sequences from a virulent strain. Southern hybridization analysis revealed a high frequency of DNA polymorphism. Restriction fragments generated by each enzyme-probe combination resulted in distinct banding patterns, clearly separating the isolates into two group

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Buse, J. B., R. Rifai-Haddad, S. Lees, et al. "Major histocompatibility complex restriction fragment length polymorphisms define three diabetogenic haplotypes in BB and BBN rats." Journal of Experimental Medicine 162, no. 2 (1985): 444–58. http://dx.doi.org/10.1084/jem.162.2.444.

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Class I and II major histocompatibility complex (MHC) probes can be used to subdivide diabetes-prone BB rats and their BBN control strain, coderived from the same outbred colony by selection against diabetes. Class II probes (A-alpha in particular) distinguish four restriction fragment length polymorphisms (RFLP), termed 1a, 1b, 2a, and 2b, in the BBN population, only one of which (2a) is found in BB rats. The degree of class II RFLP in the population studied is RT1.B-alpha greater than or equal to RT1.B-beta greater than RT1.D-alpha greater than or equal to RT1.D-beta, suggesting that intra-c

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ASHER, A. J., L. S. WALDRON, and M. L. POWER. "Rapid identification of Giardia duodenalis assemblages in NSW using terminal-restriction fragment length polymorphism." Parasitology 139, no. 8 (2012): 1005–13. http://dx.doi.org/10.1017/s0031182012000388.

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SUMMARYHumans are infected by 2 genetic assemblages (A and B) of Giardia duodenalis, a protozoan parasite that causes gastro-intestinal disease. Sub-assemblages AI, AII, BIII and BIV are commonly identified in human cases. Detection requires amplification of G. duodenalis loci. Subsequent DNA sequencing or restriction fragment length polymorphism (RFLP) identifies sub-assemblages but is expensive (DNA sequencing) or insensitive (RFLP). This study investigated a fluorescence-based detection method, using terminal-restriction fragment length polymorphism (T-RFLP) of the glutamate dehydrogenase g

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Rupe, J. C., J. C. Correll, J. C. Guerber, et al. "Differentiation of the sudden death syndrome pathogen of soybean, Fusarium solani f.sp. glycines, from other isolates of F. solani based on cultural morphology, pathogenicity, and mitochondrial DNA restriction fragment length polymorphisms." Canadian Journal of Botany 79, no. 7 (2001): 829–35. http://dx.doi.org/10.1139/b01-051.

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Forty-four isolates consisting of Fusarium solani (Mart.) Sacc. f.sp. glycines Roy, Fusarium solani f.sp. phaseoli (Burkholder) W.C. Snyder & H.N. Hans., and F. solani, collected from a variety of hosts and locations, were compared based on pathogenicity on soybean and mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs). The 24 isolates of F. solani f.sp. glycines caused more severe sudden death syndrome (SDS) foliar symptoms and root rot on soybean compared with all other isolates. All isolates of F. solani f.sp. glycines belonged to a single mtDNA RFLP haplotype.

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Lakshmi, M., M. Parani, Nivedita Ram, and Ajay Parida. "Molecular phylogeny of mangroves VI. Intraspecific genetic variation in mangrove species Excoecaria agallocha L. (Euphorbiaceae)." Genome 43, no. 1 (2000): 110–15. http://dx.doi.org/10.1139/g99-109.

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Genomic DNA from 84 individuals of Excoecaria agallocha from seven mangrove populations were analysed for random amplified polymorphic DNAs (RAPDs) using 16 random 10-mer primers. Polymorphism within populations varied from 20% to 31%. At the interpopulation level, 111/149 (74%) of RAPDs were polymorphic. Restriction fragment length polymorphism (RFLP) analysis of 21 individuals (3 individuals randomly selected from the 7 populations) using 30 probe-enzyme combinations revealed a high level of interpopulation polymorphism (62.2%) indicating interpopulation genetic divergence. The polymorphic R

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VICTOIR, K., A. L. BAÑULS, J. AREVALO, et al. "The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia." Parasitology 117, no. 1 (1998): 1–13. http://dx.doi.org/10.1017/s0031182098002789.

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In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a refe

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Smead, Deborah L., Robert L. Nussbaum, and Jennifer M. Puck. "RFLPs in human X-linked PGK1: a new probe for the PstI RFLP demonstrates strong linkage disequilibrium with the Bgll RFLP." Nucleic Acids Research 17, no. 18 (1989): 7551. http://dx.doi.org/10.1093/nar/17.18.7551.

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Tzeng, T. H., L. K. Lyngholm, C. F. Ford, and C. R. Bronson. "A restriction fragment length polymorphism map and electrophoretic karyotype of the fungal maize pathogen Cochliobolus heterostrophus." Genetics 130, no. 1 (1992): 81–96. http://dx.doi.org/10.1093/genetics/130.1.81.

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Abstract A restriction fragment length polymorphism (RFLP) map has been constructed of the nuclear genome of the plant pathogenic ascomycete Cochliobolus heterostrophus. The segregation of 128 RFLP and 4 phenotypic markers was analyzed among 91 random progeny of a single cross; linkages were detected among 126 of the markers. The intact chromosomal DNAs of the parents and certain progeny were separated using pulsed field gel electrophoresis and hybridized with probes used to detect the RFLPs. In this way, 125 markers were assigned to specific chromosomes and linkages among 120 of the markers w

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34

NENENG, Liswara, Rudy Agung NUGROHO, Yukio KOMAI, Naru TAKAYAMA, and Koji KAWAMURA. "Water Quality Measurements with a Simple Molecular Analysis (PCR-RFLP) of the Microbiome in a Metropolitan River System in Japan." Walailak Journal of Science and Technology (WJST) 17, no. 3 (2019): 257–68. http://dx.doi.org/10.48048/wjst.2020.5869.

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Urbanization has affected natural freshwater environments by contamination with sewage, toxic chemicals, and excess nutrients, which cause algal bloom, pollution, and ecosystem degradation. To ensure sustainable use of natural waters, appropriate monitoring methods are required. This study aims to investigate the diversity of the microbial community in a metropolitan river system in Japan using a low-cost DNA-based approach, PCR (Polymerase Chain Reaction)-RFLP (Restriction Fragment Length Polymorphism), as a potential bioindicator of environmental change. Surface waters were sampled in seven

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35

Prince, James P., and Steven D. Tanksley. "Restriction fragment length polymorphisms in plant breeding and genetics." Proceedings of the Royal Society of Edinburgh. Section B. Biological Sciences 99, no. 3-4 (1992): 23–29. http://dx.doi.org/10.1017/s0269727000005479.

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SynopsisThe usefulness of restriction fragment length polymorphisms (RFLPs) in plant breeding and genetics is discussed, with particular emphasis on tagging genes, map-based cloning, the assessment of genetic variability and distances, and comparative genome mapping.The Department of Plant Breeding and Biometry has currently established tight linkages between RFLPs and more than 20 genes of economic importance. Approximately half of these genes confer resistance to major pathogens including nematodes, bacteria, fungi, and viruses. Other genes tagged are involved in various aspects of crop qual

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Jackson, C. J., A. J. Fox, D. R. A. Wareing, D. N. Hutchinson, and D. M. Jones. "The application of genotyping techniques to the epidemiological analysis ofCampylobacter jejuni." Epidemiology and Infection 117, no. 2 (1996): 233–44. http://dx.doi.org/10.1017/s0950268800001400.

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SummaryCampylobacter jejuniserogroup reference strains and collections of sporadic and outbreak- associated isolates were examined for restriction fragment length polymorphisms (RFLPs), usingC. jejunirandom chromosomal and 16S rRNA gene probes. A collection of 48 Penner (HS) and 14 Lior (HL) serogroup reference strains, plus 10 clinical isolates, generated 35 RFLP and 26 ribotype patterns. In combination the two loci generated 48 distinct genotypes. Both probes were able to differentiate between certain random isolates of the same HS/HL serogroups but greater discrimination was obtained with R

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Råberg, Ulrika, Nils O. S. Högberg, and Carl Johan Land. "Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi." Holzforschung 59, no. 6 (2005): 696–702. http://dx.doi.org/10.1515/hf.2005.111.

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AbstractIn the present work PCR technology was used as a tool to detect the early stages of wood decay and was compared with microscopic evaluation. The wood decay fungiPostia placentaandConiophora puteanawere detectable in interior wood samples by terminal restriction fragment length polymorphism (T-RFLP) after 2weeks of incubation with monocultures, while microscopic detection of hyphae was not possible until after 7 weeks. A potential problem when fungal communities are studied with T-RFLPs of rDNA is that intra-specific variation complicates data analysis. In this work, we show that intra-

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Prince, James P., Vincent K. Lackney, Carmichael Angeles, James R. Blauth, and Molly M. Kyle. "A survey of DNA polymorphism within the genus Capsicum and the fingerprinting of pepper cultivars." Genome 38, no. 2 (1995): 224–31. http://dx.doi.org/10.1139/g95-027.

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Interspecific genetic variation was examined in the genus Capsicum based on shared restriction fragments in Southern analyses. Four distinct clusters were delineated among 21 accessions of cultivated and wild pepper (C. annuum, C. baccatum, C. chacoense, C. chinense, and C. frutescens). Three tight clusters comprised of accessions belonging to C. annuum, C. frutescens, and C. baccatum, respectively, were formed, along with a fourth cluster comprised of one accession each of C. chinense and C. chacoense. All accessions were differentiated by this technique, and the clusters corresponded closely

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Stalker, H. T., J. S. Dhesi, and G. Kochert. "Genetic diversity within the species Arachis duranensis Krapov. &W.C. Gregory, a possible progenitor of cultivated peanut." Genome 38, no. 6 (1995): 1201–12. http://dx.doi.org/10.1139/g95-158.

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Eighteen accessions of a diploid wild peanut species (Arachis duranensis) were analyzed using morphological, intercrossing, cytological, and RFLP data. Abundant variation was found for morphological characters and for RFLP patterns both between and within accessions, and each accession could be uniquely identified by RFLP pattern. Several plants were found to be F1 hybrids between different accessions, indicating that intercrossing had occurred when these were planted for seed increase. Patterns of RFLP diversity were found to correspond with geographic distribution. Analysis of the number of

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Pate, M., M. Moravkova, B. Krt, I. Pavlik, and M. Ocepek. "Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP." Veterinární Medicína 54, No. 6 (2009): 270–79. http://dx.doi.org/10.17221/3084-vetmed.

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ABSTRACT: Apart from birds, <I>Mycobacterium avium</I> subsp. <I>avium (MAA) </I> is often isolated from granulomatous lesions in pigs and occasionally from cattle and other animals. The objectives of this study were the detection of IS<I>901</I> restriction fragment length polymorphism (RFLP) types of <I>MAA</I> isolates from different species of domestic animals between the years 1998 and 2004 and the comparison of the detected RFLP types with previously described RFLP types collected in the database of the OIE Reference Laboratory for Avian Tu

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Reinisch, A. J., J. M. Dong, C. L. Brubaker, D. M. Stelly, J. F. Wendel, and A. H. Paterson. "A detailed RFLP map of cotton, Gossypium hirsutum x Gossypium barbadense: chromosome organization and evolution in a disomic polyploid genome." Genetics 138, no. 3 (1994): 829–47. http://dx.doi.org/10.1093/genetics/138.3.829.

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Abstract We employ a detailed restriction fragment length polymorphism (RFLP) map to investigate chromosome organization and evolution in cotton, a disomic polyploid. About 46.2% of nuclear DNA probes detect RFLPs distinguishing Gossypium hirsutum and Gossypium barbadense; and 705 RFLP loci are assembled into 41 linkage groups and 4675 cM. The subgenomic origin (A vs. D) of most, and chromosomal identity of 14 (of 26), linkage groups is shown. The A and D subgenomes show similar recombinational length, suggesting that repetitive DNA in the physically larger A subgenome is recombinationally ine

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42

Noli, Enrico, Silvio Salvi, and Roberto Tuberosa. "Comparative analysis of genetic relationships in barley based on RFLP and RAPD markers." Genome 40, no. 5 (1997): 607–16. http://dx.doi.org/10.1139/g97-080.

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Genetic relationships have seldom been analyzed with different types of molecular markers in order to compare the information provided by each marker class. We investigated genetic relationships among nine barley cultivars using separate cluster analyses based on restriction fragment length polymorphisms (RFLPs) and random amplified polymorphic DNAs (RAPDs). Genomic DNA restricted with three enzymes and hybridized with 68 probes revealed 415 RFLPs (74.2% of all bands). Among the 128 primers used for RAPD analysis, 100 provided a reproducible profile, 89 of which revealed 202 polymorphic and 56

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Alizadeh, A., M. Arlat, A. Sarrafi, C. A. Boucher, and G. Barrault. "Restriction Fragment Length Polymorphism Analyses of Iranian Strains of Xanthomonas campestris from Cereals and Grasses." Plant Disease 81, no. 1 (1997): 31–35. http://dx.doi.org/10.1094/pdis.1997.81.1.31.

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Restriction fragment length polymorphism (RFLP) analyses of the genomic DNA of 45 Xanthomonas campestris strains from cereals and grasses in Iran, and of 17 reference strains, were performed using two probes originating from X. campestris and including hrp genes. The Iranian strains studied belonged to three clearly distinct RFLP groups related to the grouping previously established on the basis of biochemical and physiological characters and host range. RFLP group 1 encompassed all the strains pathogenic to barley but not to the other plants tested (i.e., wheat, rye, Bromus inermis, Lolium mu

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Nagashima, Koji, Takayoshi Hisada, Maremi Sato, and Jun Mochizuki. "Application of New Primer-Enzyme Combinations to Terminal Restriction Fragment Length Polymorphism Profiling of Bacterial Populations in Human Feces." Applied and Environmental Microbiology 69, no. 2 (2003): 1251–62. http://dx.doi.org/10.1128/aem.69.2.1251-1262.2003.

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ABSTRACT New primer-enzyme combinations for terminal restriction fragment length polymorphism (T-RFLP) targeting of the 16S rRNA gene were constructed by using the T-RFLP analysis program (designated TAP T-RFLP) located at the Ribosomal Database Project website, and their performance was examined empirically. By using the fluorescently labeled 516f primer (Escherichia coli positions 516 to 532) and 1510r primer (positions 1510 to 1492), the 16S rRNA gene was amplified from human fecal DNA. The resulting amplified product was digested with RsaI plus BfaI or with BslI. When the T-RFLP was carrie

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Sibley, L. D., A. J. LeBlanc, E. R. Pfefferkorn, and J. C. Boothroyd. "Generation of a restriction fragment length polymorphism linkage map for Toxoplasma gondii." Genetics 132, no. 4 (1992): 1003–15. http://dx.doi.org/10.1093/genetics/132.4.1003.

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Abstract We have constructed a genetic linkage map for the parasitic protozoan, Toxoplasma gondii, using randomly selected low copy number DNA markers that define restriction fragment length polymorphisms (RFLPs). The inheritance patterns of 64 RFLP markers and two phenotypic markers were analyzed among 19 recombinant haploid progeny selected from two parallel genetic crosses between PLK and CEP strains. In these first successful interstrain crosses, these RFLP markers segregated into 11 distinct genetic linkage groups that showed close correlation with physical linkage groups previously defin

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Pappalardo, Anna Maria, Marta Giuga, Alessandra Raffa, et al. "COIBar-RFLP Molecular Strategy Discriminates Species and Unveils Commercial Frauds in Fishery Products." Foods 11, no. 11 (2022): 1569. http://dx.doi.org/10.3390/foods11111569.

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The DNA analysis is the best approach to authenticate species in seafood products and to unveil frauds based on species substitution. In this study, a molecular strategy coupling Cytochrome Oxidase I (COI) DNA barcoding with the consolidated methodology of Restriction Fragment Length Polymorphisms (RFLPs), named COIBar-RFLP, was applied for searching pattern of restriction enzyme digestion, useful to discriminate seven different fish species (juveniles of Engraulis encrasicolus and Sardina pilchardus sold in Italy as “bianchetto” and Aphia minuta sold as “rossetto”; icefish Neosalanx tangkahke

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Kam-Morgan, L. N. W., B. S. Gill, and S. Muthukrishnan. "DNA restriction fragment length polymorphisms: a strategy for genetic mapping of D genome of wheat." Genome 32, no. 4 (1989): 724–32. http://dx.doi.org/10.1139/g89-503.

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The use of restriction fragment length polymorphisms (RFLPs) as genetic markers in bread wheat, Triticum aestivum, and a wild wheat progenitor, Aegilops squarrosa, was investigated. The objectives were (i) to identify RFLP loci; (ii) to assign cDNA sequences onto specific chromosomes and chromosome arms; and (iii) to determine linkage relationships between RFLP loci. A low level of polymorphism was found, utilizing barley cDNA clones as probes, in hexaploid cultivated wheats. However, accessions of A. squarrosa revealed greater polymorphism. Wheat–barley alien addition lines were used to assig

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Halward, Tracy M., H. Thomas Stalker, Elizabeth A. Larue, and Gary Kochert. "Genetic variation detectable with molecular markers among unadapted germ-plasm resources of cultivated peanut and related wild species." Genome 34, no. 6 (1991): 1013–20. http://dx.doi.org/10.1139/g91-156.

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Peanut germ plasm consists of the cultivated allotetraploid species Arachis hypogaea L. and a large number of wild species, which are nearly all diploids. Our previous work indicated a very low level of genetic variability in American cultivars, as assayed by restriction fragment length polymorphism (RFLP) analysis. Since American cultivars might represent a narrow genetic base, we expanded our study to include unadapted germ-plasm lines from the various South American centers of origin, Africa, and China, where considerable morphological and physiological variability has been reported to exis

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Olufowote, Johnson O., Yunbi Xu, Xiuli Chen, et al. "Comparative evaluation of within-cultivar variation of rice (Oryza sativa L.) using microsatellite and RFLP markers." Genome 40, no. 3 (1997): 370–78. http://dx.doi.org/10.1139/g97-050.

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The objective of this study was to determine an efficient way of detecting within-cultivar variation in rice varieties obtained from national and international germplasm collections. Seventy-one rice cultivars were evaluated for within-cultivar variation using a combination of phenotypic, RFLP, and microsatellite or simple sequence length polymorphism (SSLP). Variation between individuals within an accession and between duplicate accessions within a cultivar was detected even in cultivars that had been purified by phenotypic evaluation. Landrace cultivars were more heterogeneous and displayed

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Nocelli, E., T. Giovannini, M. Bioni, and R. Alicchio. "RFLP- and RAPD-based genetic relationships of seven diploid species of Avena with the A genome." Genome 42, no. 5 (1999): 950–59. http://dx.doi.org/10.1139/g99-029.

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Relatively few molecular analyses are available for diploid oat species, which constitute the majority of the wild species of Avena and, therefore, the principal natural reservoir of variability. The present work reports an RAPD- (random amplified polymorphic DNA) and RFLP-(restriction fragment length polymorphism) based study of the intra- and interspecific variability of seven diploid A-genome oat species. Both types of markers resulted in valid tools for identifying polymorphisms both within and between species. The two statistical analyses, UPGMA (unweighted pair group method, arithmetic m

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