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Rozprawy doktorskie na temat „RNA-binding”

Autor: Grafiati
Data publikacji: 4 czerwca 2021
Data aktualizacji: 25 lipca 2025

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1

Maticzka, Daniel [Verfasser], and Rolf [Akademischer Betreuer] Backofen. "Modelling binding preferences of RNA-binding proteins." Freiburg : Universität, 2017. http://d-nb.info/1135134146/34.

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2

Khanal, Reecha. "Identification of RNA Binding Proteins and RNA Binding Residues Using Effective Machine Learning Techniques." ScholarWorks@UNO, 2019. https://scholarworks.uno.edu/honors_theses/128.

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Identification and annotation of RNA Binding Proteins (RBPs) and RNA Binding residues from sequence information alone is one of the most challenging problems in computational biology. RBPs play crucial roles in several fundamental biological functions including transcriptional regulation of RNAs and RNA metabolism splicing. Existing experimental techniques are time-consuming and costly. Thus, efficient computational identification of RBPs directly from the sequence can be useful to annotate RBP and assist the experimental design. Here, we introduce AIRBP, a computational sequence-based method,
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3

Deumer, Claudia D. "RNA-binding proteins in yeast mitochondria." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2002. http://nbn-resolving.de/urn:nbn:de:swb:14-1035897639531-83407.

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This work focused on the further characterisation of Idhp and of the Krebs cycle enzymes citrate synthase 1 (Cit1p) and malate dehydrogenase 1 (Mdh1p) both of which have been identified as RNA-binding proteins without known RNA recognition motifs. Besides analysing their effects on mitochondrial translation and their organisation in protein complexes the work focused on the characterisation of the RNA-binding properties of recombinant Cit1p and Mdh1p: · Cit1p and Mdh1p play no essential role in mitochondrial protein synthesis. · Idhp is in a complex of molecular weight larger than the cytochro
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4

Loushin, Newman Carrie Lee. "Characterization of QKI RNA binding function /." Full text (PDF) from UMI/Dissertation Abstracts International, 2000. http://wwwlib.umi.com/cr/utexas/fullcit?p3004323.

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5

Crombie, Catriona Ann. "Histone hairpin binding protein, an RNA binding protein, essential for development." Thesis, University of Aberdeen, 2003. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU602058.

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Histones are proteins found in the nuclei of eukaryotic cells where they are complexed to DNA in chromatin. Rephcation-dependent histones are expressed only during S-phase. Regulation of expression of replication-dependent histone genes requires a highly conserved hairpin RNA element in the 3' untranslated region of histone mRNAs. Replication-dependent histone mRNAs are not polyadenylated; their 3' end is formed by an endonucleolytic cleavage event, 3' of a hairpin element, which is recognised by the Hairpin Binding Protein, HBP (also known as Stem-Loop Binding Protein, SLBP). This protein-RNA
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6

Whittington, Christi Leigh. "Molecular Dynamics of the RNA Binding Cavity of Influenza A Non-structural Protein 1 (NS1) RNA Binding Domain." Scholar Commons, 2012. http://scholarcommons.usf.edu/etd/4256.

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Molecular dynamics simulations were performed on the influenza A non-structural protein 1 (NS1) RNA binding domain (RBD), a homodimer. Fourteen simulations were performed at 298K, nine ionized with 0.1M KCl and five with no ions. Several analysis techniques were employed to study RBD residue flexibility. The focus of the study was the RNA binding cavity formed by side chains of helix 2 (chain A) and helix 2’ (chain B) and cavity intermonomeric salt bridges. Opening of the salt bridges D29–R46’ and D29’–R46 was observed in several of the trajectories. The RNA binding cavity has large flexibilit
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7

Blancafort, Pilar. "Making conformation-specific RNA-binding zinc fingers." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape7/PQDD_0023/NQ47598.pdf.

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8

Waterman, David Geoffrey. "Structural studies on prokaryotic RNA-binding proteins." Thesis, University of York, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.441058.

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9

Fesser, Stephanie Marion. "Contribution of RNA binding proteins to substrate specificity in small RNA biogenesis." Diss., Ludwig-Maximilians-Universität München, 2013. http://nbn-resolving.de/urn:nbn:de:bvb:19-173105.

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10

Attig, Jan. "Impact of retrotransposon-derived RNA elements and their recognition by RNA binding proteins." Thesis, University of Cambridge, 2015. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.709161.

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11

Brackett, David Michael. "Ligand binding and catalysis in an RNA aptamer /." For electronic version search Digital dissertations database. Restricted to UC campuses. Access is free to UC campus dissertations, 2005. http://uclibs.org/PID/11984.

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12

Hill, G. R. "NMR studies of DNA and RNA binding proteins." Thesis, University of Cambridge, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.604060.

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HMG-D is a 112-residue, non-histone chromosomal protein from <i>Drosophila melanogaster </i>and is a member of the class of non-sequence specific HMGB proteins. The present project was based on the observation that other HMGB complexes that had been solved by NMR had a phenylalanine residue at a key interfacial location (corresponding to position 12 in HMG-D), whereas those like HMG-D that gave few intermolecular NOE cross peaks generally had a tyrosine at this location. This tyrosine was known to be involved in hydrogen-bonding to the DNA in a related complex that had been solved crystallogra
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13

Sang, Andrea Elizabeth. "Molecular and physiological studies of RNA-binding proteins." Thesis, University of Newcastle Upon Tyne, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244469.

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14

Robertson, Alexander De Jong. "Understanding regulation of mRNA by RNA binding proteins." Thesis, Massachusetts Institute of Technology, 2014. http://hdl.handle.net/1721.1/87474.

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Thesis: Ph. D., Massachusetts Institute of Technology, Computational and Systems Biology Program, 2014.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references (pages 167-187).<br>Posttranscriptional regulation of mRNA by RNA-binding proteins plays key roles in regulating the transcriptome over the course of development, between tissues and in disease states. The specific interactions between mRNA and protein are controlled by the proteins' inherent affinities for different RNA sequences as well as other features such as translation and RNA structure which affect the ac
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15

Wolf, Joshua Jaeger. "Post-transcriptional coordination by an RNA-binding protein." Thesis, Massachusetts Institute of Technology, 2010. http://hdl.handle.net/1721.1/57893.

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Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2010.<br>Cataloged from PDF version of thesis.<br>Includes bibliographical references.<br>RNA-binding proteins can regulate the stability, localization, and translation of their target mRNAs. Post-transcriptional regulation can orchestrate dynamic changes in gene expression, and can coordinate multiple cellular processes in response to various stimuli. Filamentous growth in Saccharomyces cerevisiae is a morphogenetic switch that occurs in response to nitrogen starvation and requires alterations in cell growth, cell cycle
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16

Brugiolo, Mattia. "The dynamic RNA-binding behavior of SR proteins." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-191373.

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In the cell, the genetic information encoded in the DNA is transcribed to RNA. All RNAs that are transcribed in the cell are initially produced as precursor RNAs, which have to undergo various steps of processing to obtain their mature form. The maturation and processing for all RNA classes requires the activity of multiple RNA binding proteins (RBPs). An important family of RBPs that is involved in RNA maturation and processing is the SR-protein family. SR proteins are important for the regulation of a multitude of processes that include: splicing, transcription, export, RNA stabilization,
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17

Zinnall, Ulrike. "Functional characterization of the RNA-binding protein HDLBP." Doctoral thesis, Humboldt-Universität zu Berlin, 2021. http://dx.doi.org/10.18452/23301.

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Der Sekretionsweg ist essenziell für die Funktion von Zellen und beginnt, wenn mRNAs, die für Membran- und Sekretionsproteine codieren, an das endoplasmatische Retikulum (ER) gebracht werden. Allerdings ist wenig darüber bekannt, inwiefern RNA-bindende Proteine zur Erkennung und Translation von ER lokalisierten mRNAs beitragen. In dieser Arbeit haben wir das humane RNA-bindende Protein HDLBP charakterisiert. Wir haben durch PAR-CLIP-, Zellfraktionierungs- und RNA-Seqeuenzierexperimente festgestellt, dass HDLBP an mehr als 80% aller ER lokalisierten mRNAs bindet. Analysen zu HDLBPs Bindungsmo
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18

Morris, Katherine Louise. "Investigation of RNA binding proteins regulated by mTOR." Thesis, University of Leicester, 2017. http://hdl.handle.net/2381/39742.

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The mammalian target of rapamycin (mTOR) is a serine/threonine protein kinase which plays a key role in the transduction of cellular energy signals, in order to coordinate and regulate a wide number of processes including cell growth and proliferation via control of protein synthesis and protein degradation. For a number of human diseases where mTOR signalling is dysregulated, including cancer, the clinical relevance of mTOR inhibitors is clear. However, understanding of the mechanisms by which mTOR controls gene expression is incomplete, with implications for adverse toxicological effects of
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19

Dwyer, Holly. "Characterization of putative Porphyromonas gingivalis RNA-binding proteins." VCU Scholars Compass, 2014. http://scholarscompass.vcu.edu/etd/3550.

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Porphyromonas gingivalis (P. gingivalis) is a gram-negative, anaerobic bacterium recognized as a major player in progression of periodontal disease. P. gingivalis survives in the oral cavity while being exposed to dynamic environmental conditions such as pH, temperature, nutrient availability and host immune responses such as oxygen tension and nitrosative stress. Survival and pathogenesis of P. gingivalis in the oral cavity require mechanisms to regulate gene expression in response to the extracellular signals. Little is known about the regulatory mechanisms of P. gingivalis in the oral cavit
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20

Collins, K. M. "Target recognition by multi-domain RNA-binding proteins." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1460867/.

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Multi-functional RNA binding proteins regulate and coordinate the many steps of RNA metabolism. Accurate functioning of these processes is vital in cells and misregulation has been linked to many human diseases. RNA binding proteins contain multiple RNA binding domains. The ability to perform multiple functions depends on the recognition of a diverse range of targets and domains are used combinatorially to achieve this. In this thesis I ask how the sequence specificity of low affinity RNA-binding domains and the interplay between said domains plays a role in RNA target selectivity. Within this
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21

Wang, Hai. "Design, synthesis and RNA binding of aminoglycoside antibiotics /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 1998. http://wwwlib.umi.com/cr/ucsd/fullcit?p9820848.

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22

Moro, Alberto Maria. "Functional characterization of the RNA binding protein RALY." Doctoral thesis, University of Trento, 2013. http://eprints-phd.biblio.unitn.it/1086/1/Albertomaria_Moro_thesis_Final_version.pdf.

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Of 25000 genes encoded from genome, more than 90% are subject to alternative splicing or other post-transcriptional modifications. All these events produce a high number of different proteins that form the basis for the high variety of cells. The RNAbinding proteins (RBPs) play crucial roles in this variability by regulating many steps of biological processes regarding RNA metabolism. The heterogeneous nuclear ribonucleoproteins (hnRNPs) belong to big family of RBPs involved in many aspects of RNA metabolism including RNA stability, intracellular transport and translation. More recently, RALY,
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23

Malaurie, Emilie. "Structural and ligand binding studies of EDEN-BP and CPEB1 RNA binding proteins." Thesis, University of Nottingham, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.546689.

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24

Stark, Jeremy M. "SR proteins can function during alternative splicing to mediate exon/exon associations /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/5020.

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25

Zhong, Jun. "A double-stranded RNA binding protein that is important for murine spermatogenesis and growth /." Thesis, Connect to this title online; UW restricted, 1999. http://hdl.handle.net/1773/10301.

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26

Kok, Kin-hang. "Roles of human double-stranded RNA binding proteins TRBP and PACT in RNA interference." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B38523218.

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27

Kok, Kin-hang, and 郭健恆. "Roles of human double-stranded RNA binding proteins TRBP and PACT in RNA interference." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B38523218.

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28

Best, Andrew Jonathan. "Identification and characterisation of RNA targets of the RNA binding proteins TRa2a and TRa2B." Thesis, University of Newcastle upon Tyne, 2014. http://hdl.handle.net/10443/2775.

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Alternative splicing – the production of multiple messenger RNA isoforms from a single gene – is regulated in part by RNA binding proteins. The overall aim of this study was to identify and characterise novel targets of the RNA binding proteins Tra2α and Tra2β, in order to further understand their biological functions. Tra2β is implicated in male germ cell development and in the initial stages of this project, I utilised data from a previous Tra2β HITS-CLIP experiment to validate and characterise novel RNA targets from the mouse testis using minigenes. These included a large testis-enriched ex
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29

Kylberg, Karin. "Transcription and transport of a messenger RNP particle : novel regulatory mechanisms /." Stockholm : Karolinska institutet, 2007. http://diss.kib.ki.se/2007/978-91-7357-318-4/.

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30

Silva, Ana. "Structural and functional studies of several RNA-binding proteins." Thesis, University of York, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.516585.

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31

Todorova, Tanya (Tanya Todorova). "Function and regulation of PARP13 binding to cellular RNA." Thesis, Massachusetts Institute of Technology, 2015. http://hdl.handle.net/1721.1/97789.

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Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 2015.<br>Cataloged from PDF version of thesis. Vita.<br>Includes bibliographical references.<br>Poly(ADP-ribose) polymerase-13 (PARP13) is a member of the PARP family of proteins - enzymes that use NAD+ to synthesize a posttranslational protein modification called poly(ADP-ribose) (PAR). PARPs function in multiple cellular pathways, and recently several members of the family have been implicated in regulating various steps in RNA metabolism, from splicing to translation and decay. PARP1 3 is the best-understood RNA-r
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32

Freese, Peter (Peter Dale). "Biochemical and functional characterization of human RNA binding proteins." Thesis, Massachusetts Institute of Technology, 2018. http://hdl.handle.net/1721.1/115601.

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Thesis: Ph. D., Massachusetts Institute of Technology, Computational and Systems Biology Program, 2018.<br>This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.<br>Cataloged from student-submitted PDF version of thesis.<br>Includes bibliographical references (pages 205-226).<br>RNA not only shuttles information between DNA and proteins but also carries out many other essential cellular functions. Nearly all steps of an RNA's life cycle are controlled by approximately one thousand RNA binding proteins (
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33

Kheirollahi, Kouhestani Mahsa. "Analysis of RNA binding proteins using stable cell lines." Thesis, University of Newcastle upon Tyne, 2014. http://hdl.handle.net/10443/2504.

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Over 94% of human genes are alternatively spliced. Alternative splicing regulation is necessary for some developmental pathways. Many proteins including RNA binding proteins such as STAR family, hnRNPs, SR and SR-like proteins are involved in alternative splicing regulation. Some members of these proteins also have important roles in gene expression, transcription, signal transduction, RNA metabolism, cell cycle regulation and cancer. Although these proteins are known to be involved in alternative splicing control of specific targets, mechanisms of many of their actions and protein interaction
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34

Brown, James W. "RNA polymerase binding sites and polyadenylated RNAs in archaebacteria /." The Ohio State University, 1988. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487591658174843.

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35

SPATARO, CLARISSA. "IDENTIFICATION OF NEW MYC DEPENDENCIES AMONG RNA-BINDING PROTEINS." Doctoral thesis, Università degli Studi di Milano, 2022. http://hdl.handle.net/2434/909490.

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Increased expression and activity of the MYC protein is a widespread cancer hallmark and renders tumor cells addicted to sustained activation of a variety of other gene products. Identification of those dependencies can offer new therapeutic approaches against MYC-driven tumors. Previous studies showed that RNA processing events have a critical role in MYC-induced tumorigenesis and survival. Moreover, we and others observed that multiple genes encoding RNABinding Proteins (RBPs) were positively regulated upon MYC activation in various cell types. Hence, we hypothesized that the activity
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36

DeLaney, Elizabeth Erin. "RNA Recognition by the Pattern Recognition Receptor RIG-I: Roles of RNA Binding, Multimerization, and RNA-dependent ATPase Activity." Case Western Reserve University School of Graduate Studies / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=case1405015903.

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37

Cass, Danielle Marie. "Role of two RNA binding properties in pre-mRNA splicing /." view abstract or download file of text, 2007. http://proquest.umi.com/pqdweb?did=1400950811&sid=2&Fmt=2&clientId=11238&RQT=309&VName=PQD.

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Thesis (Ph. D.)--University of Oregon, 2007.<br>Typescript. Includes vita and abstract. Includes bibliographical references (leaves 67-80). Also available for download via the World Wide Web; free to University of Oregon users.
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38

Long, Jennifer Connie. "Identification of in vivo RNA tragets of the RNA-binding proteins Acinus and hnRNP A1." Thesis, University of Edinburgh, 2009. http://hdl.handle.net/1842/4227.

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RNA-binding proteins play a central role in the post-transcriptional regulation of gene expression; however, little is known about the endogenous transcripts to which they bind. Here, I have used the ultra-violet cross-linking and immuno-precipitation (CLIP) technique to identify RNA targets directly bound to two RNA-binding proteins: Acinus and hnRNP A1. Acinus (apoptotic chromatin condensation inducer in the nucleus) contains a region that is homologous to the RNA binding domain of the Drosophila splicing regulator sex-lethal, and a serine and arginine rich region similar to that seen in the
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39

Howe, Peter W. A. "NMR studies of the RNA binding domain of U1A protein and its complexes with RNA." Thesis, University of Cambridge, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313879.

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40

Nashchekin, Dmitri. "A Y-box protein/RNA helicase complex links mRNP assembly on the gene to mRNA translation /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-811-8/.

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41

Nguyen, Loc Tien. "BIV TAR RNA binding glycine mutant Tat peptides| An integrated modeling and binding assay approach." Thesis, San Jose State University, 2015. http://pqdtopen.proquest.com/#viewpdf?dispub=1602943.

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<p> Interactions between viral encoded regulatory proteins and RNA target sequences control gene expression of Lentiviruses, including human immunodeficiency virus (HIV). &nbsp;Bovine immunodeficiency virus (BIV) provides a simpler model of interaction between the viral trans-activating protein (Tat) and trans-activation response RNA element (TAR), using Tat peptides binding to TAR RNA fragments. The resulting characterization of the hinge region of native BIV TAR-Tat complex was confirmed by more comprehensive calculations, involving an exhaustive generation of lattice chains. This modeled
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42

Prichard, Lisa. "The role of the IQ motif, a protein kinase C and calmodulin regulatory domain, in neuroplasticity, RNA processing, and RNA metabolism /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/6302.

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43

Sidiqi, Mahjooba. "The structure and RNA-binding of poly (C) protein 1." University of Western Australia. School of Biomedical, Biomolecular and Chemical Sciences, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0077.

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[Truncated abstract] Regulation of mRNA stability is an important posttranscriptional mechanism involved in the control of gene expression. The rate of mRNA decay can differ greatly from one mRNA to another and may be regulated by RNA-protein interactions. A key determinant of mRNA decay are sequence instability (cis) elements often located in the 3' untranslated region (UTR) of many mRNAs. For example, the AU rich elements (AREs), are such well characterized elements, and most commonly involved in promoting mRNA degradation, and specific binding of proteins to these elements leading to the st
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44

Agostini, Federico 1985. "Predictions of RNA-binding ability and aggregation propensity of proteins." Doctoral thesis, Universitat Pompeu Fabra, 2014. http://hdl.handle.net/10803/318159.

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RNA-binding proteins (RBPs) control the fate of a multitude of coding and non-coding transcripts. Formation of ribonucleoprotein (RNP) complexes fine-tunes regulation of post-transcriptional events and influences gene expression. Recently, it has been observed that non-canonical proteins with RNA-binding ability are enriched in structurally disordered and low-complexity regions that are generally involved in functional and dysfunctional associations. Therefore, it is possible that interactions with RNA protect unstructured protein domains from aberrant associations or aggregation. Nevertheless
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45

Zhang, Tong. "Characterization of the shuttling properties of RNA-binding TIA proteins." Doctoral thesis, Universite Libre de Bruxelles, 2005. http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/210999.

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46

Durie, Danielle. "RNA Binding Protein HuR Regulates the Expression of Bcl-xL." Thèse, Université d'Ottawa / University of Ottawa, 2012. http://hdl.handle.net/10393/23206.

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The RNA-binding protein HuR controls key cellular processes by binding target mRNAs and regulating them at various post-transcriptional levels. HuR can function as an Internal Ribosome Entry Site (IRES) trans-acting factor that regulates the IRES-mediated translation of XIAP. Since XIAP and Bcl-xL expression was reported to be co-regulated, we investigated whether HuR is also a regulat or of Bcl-xL expression. We found that HuR binds the 3’end of the Bcl-xL 5’UTR in-vitro. In U2OS cells, we showed that loss of HuR by siRNA significantly increased Bcl-xL protein expression while Bcl-2 and Mcl-1
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47

Swale, Christopher. "RNA binding and assembly of human influenza A virus polymerases." Thesis, Université Grenoble Alpes (ComUE), 2015. http://www.theses.fr/2015GREAV053/document.

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Le virus de la grippe A est un virus à ARN négatif appartenant à la famille des Orthomyxoviriadea dont la réplication se produit dans le noyau des cellules infectées. L'organisation du génome est segmentée en huit segments d'ARNv de polarité négative, codant pour un minimum de 16 protéines virales différentes. Ces ARN viraux (ARNv) sont en complexe avec de nombreuses copies de nucléoprotéines et liés par leurs extrémités 5' et 3' au complexe hétérotrimérique de l'ARN-polymérase ARN-dépendante composé des sous unités PA, PB1 et PB2. Cet assemblage macromoléculaire (ARNv / polymérase / NP) nommé
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48

Valeiras, Brenda. "Probing RNA binding specificities of AID/APOBEC proteins by iCLIP." Thesis, University of Cambridge, 2019. https://www.repository.cam.ac.uk/handle/1810/288562.

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The AID/APOBEC protein family comprises a group of cytosine deaminases found in vertebrates that are capable of modifying cytosine to uracil in the context of RNA or singlestranded DNA. They exert diverse valuable physiological functions including antibody diversification and restriction of viral infection. However, off-target mutations have also been shown to contribute to cancer development, making it crucial to better understand the interactions and mechanisms that regulate AID/APOBEC activity and editing site fidelity. In this regard, a new focus on RNA as a putative regulator of AID/APOBE
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Spellman, Rachel Claudine Helen. "Investigation of polypyrimidine tract binding protein function by RNA interference." Thesis, University of Cambridge, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.614210.

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Zinnall, Ulrike [Verfasser]. "Functional characterization of the RNA-binding protein HDLBP / Ulrike Zinnall." Berlin : Humboldt-Universität zu Berlin, 2021. http://d-nb.info/1241116946/34.

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