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Artykuły w czasopismach na temat „Sigme proteins”

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Data publikacji: 6 września 2023

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1

Raman, Sahadevan, Xiaoling Puyang, Tan-Yun Cheng, David C. Young, D. Branch Moody, and Robert N. Husson. "Mycobacterium tuberculosis SigM Positively Regulates Esx Secreted Protein and Nonribosomal Peptide Synthetase Genes and Down Regulates Virulence-Associated Surface Lipid Synthesis." Journal of Bacteriology 188, no. 24 (2006): 8460–68. http://dx.doi.org/10.1128/jb.01212-06.

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ABSTRACT The Mycobacterium tuberculosis genome encodes 12 alternative sigma factors, several of which regulate stress responses and are required for virulence in animal models of acute infection. In this work we investigated M. tuberculosis SigM, a member of the extracytoplasmic function subfamily of alternative sigma factors. This sigma factor is expressed at low levels in vitro and does not appear to function in stress response regulation. Instead, SigM positively regulates genes required for the synthesis of surface or secreted molecules. Among these are genes encoding two pairs of Esx secr
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Nakunst, Diana, Christof Larisch, Andrea T. Hüser, Andreas Tauch, Alfred Pühler, and Jörn Kalinowski. "The Extracytoplasmic Function-Type Sigma Factor SigM of Corynebacterium glutamicum ATCC 13032 Is Involved in Transcription of Disulfide Stress-Related Genes." Journal of Bacteriology 189, no. 13 (2007): 4696–707. http://dx.doi.org/10.1128/jb.00382-07.

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ABSTRACT The gene for the extracytoplasmic function (ECF) sigma factor SigM was deleted from the chromosome of the gram-positive soil bacterium Corynebacterium glutamicum to elucidate the role of the SigM protein in the regulation of gene expression. Comparative DNA microarray hybridizations of the C. glutamicum wild type and sigM-deficient mutant C. glutamicum DN1 revealed 23 genes with enhanced expression in the sigM-proficient strain, encoding functions in the assembly of iron-sulfur clusters (suf operon), thioredoxin reductase (trxB), thioredoxins (trxC, trxB1), chaperones (groES, groEL, c
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Fernandes, Norvin D., Qi-long Wu, Dequan Kong, Xiaoling Puyang, Sumeet Garg, and Robert N. Husson. "A Mycobacterial Extracytoplasmic Sigma Factor Involved in Survival following Heat Shock and Oxidative Stress." Journal of Bacteriology 181, no. 14 (1999): 4266–74. http://dx.doi.org/10.1128/jb.181.14.4266-4274.1999.

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ABSTRACT Extracytoplasmic function (ECF) sigma factors are a heterogeneous group of alternative sigma factors that regulate gene expression in response to a variety of conditions, including stress. We previously characterized a mycobacterial ECF sigma factor, SigE, that contributes to survival following several distinct stresses. A gene encoding a closely related sigma factor, sigH, was cloned fromMycobacterium tuberculosis and Mycobacterium smegmatis. A single copy of this gene is present in these and other fast- and slow-growing mycobacteria, including M. fortuitum and M. avium. While the M.
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Singh, Rakesh Kumar, Lav Kumar Jaiswal, Tanmayee Nayak, et al. "Expression, Purification, and In Silico Characterization of Mycobacterium smegmatis Alternative Sigma Factor SigB." Disease Markers 2022 (May 20, 2022): 1–11. http://dx.doi.org/10.1155/2022/7475704.

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Sigma factor B (SigB), an alternative sigma factor (ASF), is very similar to primary sigma factor SigA (σ70) but dispensable for growth in both Mycobacterium smegmatis (Msmeg) and Mycobacterium tuberculosis (Mtb). It is involved in general stress responses including heat, oxidative, surface, starvation stress, and macrophage infections. Despite having an extremely short half-life, SigB tends to operate downstream of at least three stress-responsive extra cytoplasmic function (ECF) sigma factors (SigH, SigE, SigL) and SigF involved in multiple signaling pathways. There is very little informatio
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5

White, Mark J., Hongjun He, Renee M. Penoske, Sally S. Twining, and Thomas C. Zahrt. "PepD Participates in the Mycobacterial Stress Response Mediated through MprAB and SigE." Journal of Bacteriology 192, no. 6 (2010): 1498–510. http://dx.doi.org/10.1128/jb.01167-09.

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ABSTRACT Currently, one-third of the world's population is believed to be latently infected with Mycobacterium tuberculosis. The mechanisms by which M. tuberculosis establishes latent infection remain largely undefined. mprAB encodes a two-component signal transduction system required by M. tuberculosis for aspects of persistent infection. MprAB regulates a large and diverse group of genetic determinants in response to membrane stress, including the extracytoplasmic function (ECF) sigma factor sigE and the HtrA-like serine protease pepD. Recent studies have demonstrated that PepD functions as
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6

Kim, Eun Sook, Ju Yeon Song, Dae Wi Kim, Keith F. Chater, and Kye Joon Lee. "A Possible Extended Family of Regulators of Sigma Factor Activity in Streptomyces coelicolor." Journal of Bacteriology 190, no. 22 (2008): 7559–66. http://dx.doi.org/10.1128/jb.00470-08.

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ABSTRACT SCO4677 is one of a large number of similar genes in Streptomyces coelicolor that encode proteins with an HATPase_c domain resembling that of anti-sigma factors such as SpoIIAB of Bacillus subtilis. However, SCO4677 is not located close to genes likely to encode a cognate sigma or anti-anti-sigma factor. SCO4677 was found to regulate antibiotic production and morphological differentiation, both of which were significantly enhanced by the deletion of SCO4677. Through protein-protein interaction screening of candidate sigma factor partners using the yeast two-hybrid system, SCO4677 prot
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7

Kazmierczak, Mark J., Martin Wiedmann, and Kathryn J. Boor. "Alternative Sigma Factors and Their Roles in Bacterial Virulence." Microbiology and Molecular Biology Reviews 69, no. 4 (2005): 527–43. http://dx.doi.org/10.1128/mmbr.69.4.527-543.2005.

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SUMMARY Sigma factors provide promoter recognition specificity to RNA polymerase holoenzyme, contribute to DNA strand separation, and then dissociate from the core enzyme following transcription initiation. As the regulon of a single sigma factor can be composed of hundreds of genes, sigma factors can provide effective mechanisms for simultaneously regulating expression of large numbers of prokaryotic genes. One newly emerging field is identification of the specific roles of alternative sigma factors in regulating expression of virulence genes and virulence-associated genes in bacterial pathog
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8

S Thompson, L., and E. J Harry. "Alternative sigma factors: the master regulators." Microbiology Australia 27, no. 3 (2006): 118. http://dx.doi.org/10.1071/ma06118.

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When a bacterial cell encounters a change in environmental conditions, it responds by producing a different complement of cellular proteins. Which proteins are produced and maintained is regulated in a number of ways, including regulation of gene transcription, stabilising or degrading mRNA transcripts, post translational modifications and targeted degradation of proteins.
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9

Yang, Fumeng, Wenjun Wang, Qian Liu, et al. "The application of Six Sigma to perform quality analyses of plasma proteins." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 57, no. 2 (2019): 121–27. http://dx.doi.org/10.1177/0004563219892023.

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Background The Six Sigma theory is an important tool for laboratory quality management. It has been widely used in clinical chemistry, haematology and other disciplines. The aim of our study was to evaluate the analytical performance of plasma proteins by application of Sigma metric and to compare the differences among three different allowable total errors in evaluating the analytical performance of plasma proteins. Methods Three different allowable total error values were used as quality goals. Data from an external quality assessment were used as bias, and the cumulative coefficient of vari
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10

Mortillaro, N. A., and A. E. Taylor. "Microvascular permeability to endogenous plasma proteins in the jejunum." American Journal of Physiology-Heart and Circulatory Physiology 258, no. 6 (1990): H1650—H1654. http://dx.doi.org/10.1152/ajpheart.1990.258.6.h1650.

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Steady-state lymph flow and lymph (CL) and plasma (CP) protein concentrations were measured at venous outflow pressures of 0, 10, 20, and 30 mmHg in an autoperfused segment of cat jejunum. In addition to determining total protein concentrations in lymph and plasma, polyacrylamide gradient gel electrophoresis was used to determine lymph and plasma protein concentrations of albumin and nine other plasma proteins. The osmotic reflection coefficient (sigma d) for total proteins, albumin, and each of the nine protein fractions was estimated using CL/CP at a capillary filtration rate independent sta
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11

Parker, R. E., R. J. Roselli, and K. L. Brigham. "Effects of prolonged elevated microvascular pressure on lung fluid balance in sheep." Journal of Applied Physiology 58, no. 3 (1985): 869–75. http://dx.doi.org/10.1152/jappl.1985.58.3.869.

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Experiments were conducted in seven chronically instrumented unanesthetized sheep to estimate the osmotic reflection coefficient (sigma d) for total proteins and the solvent-drag reflection coefficients (sigma f) for six endogenous protein fractions. We measured the lymph-to-plasma ratio of total proteins (CL/CP) and six protein fractions during base-line conditions and after left atrial pressure elevations of 24–26 h per elevation. We also monitored pulmonary arterial pressure, left atrial pressure, systemic arterial pressure, and lung lymph flow at the various levels of pulmonary microvascul
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12

Wilson, Megan J., and Iain L. Lamont. "Mutational Analysis of an Extracytoplasmic-Function Sigma Factor To Investigate Its Interactions with RNA Polymerase and DNA." Journal of Bacteriology 188, no. 5 (2006): 1935–42. http://dx.doi.org/10.1128/jb.188.5.1935-1942.2006.

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ABSTRACT The extracytoplasmic-function (ECF) family of sigma factors comprises a large group of proteins required for synthesis of a wide variety of extracytoplasmic products by bacteria. Residues important for core RNA polymerase (RNAP) binding, DNA melting, and promoter recognition have been identified in conserved regions 2 and 4.2 of primary sigma factors. Seventeen residues in region 2 and eight residues in region 4.2 of an ECF sigma factor, PvdS from Pseudomonas aeruginosa, were selected for alanine-scanning mutagenesis on the basis of sequence alignments with other sigma factors. Fourte
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13

Negrini, D., D. Venturoli, M. I. Townsley, and R. K. Reed. "Permeability of parietal pleura to liquid and proteins." Journal of Applied Physiology 76, no. 2 (1994): 627–33. http://dx.doi.org/10.1152/jappl.1994.76.2.627.

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The permselectivity of the parietal pleura was determined in spontaneously breathing anesthetized rabbits and dogs. In rabbits, we injected intrapleurally 5 ml of 1-g/dl albumin solution containing 100 microCi of 131I-labeled albumin plus 100 microCi of either lactate dehydrogenase (LDH) or alpha 2–125I-macroglobulin. Dogs received 100 ml of 1-g/dl albumin solution containing 100 microCi of 131I-albumin plus 100 microCi of alpha 2–125I-macroglobulin. A transpleural pressure gradient was set, lowering the intracapsular pressure to -30 cmH2O. The solvent drag reflection coefficients (sigma f) we
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14

Maron, M. B., and C. F. Pilati. "Calculation of the reflection coefficient from measurements of endogenous vascular indicators." Journal of Applied Physiology 64, no. 4 (1988): 1746–48. http://dx.doi.org/10.1152/jappl.1988.64.4.1746.

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The solvent drag reflection coefficient (sigma) for total proteins can be estimated by comparing the relative degrees of concentration of erythrocytes and plasma proteins that occur during fluid filtration in an isolated perfused organ. In this analysis, we evaluated the accuracy of equations proposed by Pilati and Maron [Am. J. Physiol. 247 (Heart Circ. Physiol. 16): H1-H7, 1984] and Wolf et al. [Am. J. Physiol. 253 (Heart Circ. Physiol. 22): H194-H204, 1987] to calculate sigma from these concentration changes. We calculated sigma with each equation using data generated from a mathematical mo
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15

Liberek, K., and C. Georgopoulos. "Autoregulation of the Escherichia coli heat shock response by the DnaK and DnaJ heat shock proteins." Proceedings of the National Academy of Sciences 90, no. 23 (1993): 11019–23. http://dx.doi.org/10.1073/pnas.90.23.11019.

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All organisms respond to various forms of stress, including heat shock. The heat shock response has been universally conserved from bacteria to humans. In Escherichia coli the heat shock response is under the positive transcriptional control of the sigma 32 polypeptide and involves transient acceleration in the rate of synthesis of a few dozen genes. Three of the heat shock genes--dnaK, dnaJ, and grpE--are special because mutations in any one of these lead to constitutive levels of heat shock gene expression, implying that their products negatively autoregulate their own synthesis. The DnaK, D
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16

Gao, Forson, Amy E. Danson, Fuzhou Ye, Milija Jovanovic, Martin Buck, and Xiaodong Zhang. "Bacterial Enhancer Binding Proteins—AAA+ Proteins in Transcription Activation." Biomolecules 10, no. 3 (2020): 351. http://dx.doi.org/10.3390/biom10030351.

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Bacterial enhancer-binding proteins (bEBPs) are specialised transcriptional activators. bEBPs are hexameric AAA+ ATPases and use ATPase activities to remodel RNA polymerase (RNAP) complexes that contain the major variant sigma factor, σ54 to convert the initial closed complex to the transcription competent open complex. Earlier crystal structures of AAA+ domains alone have led to proposals of how nucleotide-bound states are sensed and propagated to substrate interactions. Recently, the structure of the AAA+ domain of a bEBP bound to RNAP-σ54-promoter DNA was revealed. Together with structures
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17

Alhadlaq, Meshari Ahmed, Jeffrey Green, and Bassam K. Kudhair. "Analysis of Kytococcus sedentarius Strain Isolated from a Dehumidifier Operating in a University Lecture Theatre: Systems for Aerobic Respiration, Resisting Osmotic Stress, and Sensing Nitric Oxide." Microbial Physiology 31, no. 2 (2021): 135–45. http://dx.doi.org/10.1159/000512751.

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A strain of <i>Kytococcus sedentarius</i> was isolated from a dehumidifier operating in a university lecture theatre. Genome analysis and phenotypic characterisation showed that this strain, <i>K. sedentarius</i> MBB13, was a moderately halotolerant aerobe with a branched aerobic electron transport chain and genes that could contribute to erythromycin resistance. The major compatible solute was glycine betaine, with ectoine and proline being deployed at higher osmolarities. Actinobacteria possess multiple WhiB-like (Wbl) regulatory proteins, and <i>K. sedentarius&
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18

Kuwana, Ritsuko, Satoko Yamamura, Hiromi Ikejiri, et al. "Bacillus subtilis spoVIF (yjcC) gene, involved in coat assembly and spore resistance." Microbiology 149, no. 10 (2003): 3011–21. http://dx.doi.org/10.1099/mic.0.26432-0.

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In systematic screening four sporulation-specific genes, yjcA, yjcB, yjcZ and yjcC, of unknown function were found in Bacillus subtilis. These genes are located just upstream of the cotVWXYZ gene cluster oriented in the opposite direction. Northern blot analysis showed that yjcA was transcribed by the SigE RNA polymerase beginning 2 h (t 2) after the onset of sporulation, and yjcB, yjcZ and yjcC were transcribed by the SigK RNA polymerase beginning at t 4 of sporulation. The transcription of yjcZ was dependent on SigK and GerE. The consensus sequences of the appropriate sigma factors were foun
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Huang, Pi H., Ying J. Li, Yu P. Su, Long H. Lee, and Hung J. Liu. "Epitope mapping and functional analysis of sigma A and sigma NS proteins of avian reovirus." Virology 332, no. 2 (2005): 584–95. http://dx.doi.org/10.1016/j.virol.2004.12.005.

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da Silva Neto, José F., Tie Koide, Suely L. Gomes, and Marilis V. Marques. "The Single Extracytoplasmic-Function Sigma Factor of Xylella fastidiosa Is Involved in the Heat Shock Response and Presents an Unusual Regulatory Mechanism." Journal of Bacteriology 189, no. 2 (2006): 551–60. http://dx.doi.org/10.1128/jb.00986-06.

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ABSTRACT Genome sequence analysis of the bacterium Xylella fastidiosa revealed the presence of two genes, named rpoE and rseA, predicted to encode an extracytoplasmic function (ECF) sigma factor and an anti-sigma factor, respectively. In this work, an rpoE null mutant was constructed in the citrus strain J1a12 and shown to be sensitive to exposure to heat shock and ethanol. To identify the X. fastidiosa σE regulon, global gene expression profiles were obtained by DNA microarray analysis of bacterial cells under heat shock, identifying 21 σE-dependent genes. These genes encode proteins belongin
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McQuade, Ryan S., Natalia Comella, and Alan D. Grossman. "Control of a Family of Phosphatase Regulatory Genes (phr) by the Alternate Sigma Factor Sigma-H ofBacillus subtilis." Journal of Bacteriology 183, no. 16 (2001): 4905–9. http://dx.doi.org/10.1128/jb.183.16.4905-4909.2001.

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ABSTRACT A family of 11 phosphatases can help to modulate the activity of response regulator proteins in Bacillus subtilis. Downstream of seven of the rap (phosphatase) genes arephr genes, encoding secreted peptides that function as phosphatase regulators. By using fusions to lacZ and primer extension analysis, we found that six of the sevenphr genes are controlled by the alternate sigma factor sigma-H. These results expand the potential of sigma-H to contribute to the output of several response regulators by controlling expression of inhibitors of phosphatases.
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Ostrov, David A., Andrew P. Bluhm, Danmeng Li, et al. "Highly Specific Sigma Receptor Ligands Exhibit Anti-Viral Properties in SARS-CoV-2 Infected Cells." Pathogens 10, no. 11 (2021): 1514. http://dx.doi.org/10.3390/pathogens10111514.

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(1) Background: There is a strong need for prevention and treatment strategies for COVID-19 that are not impacted by SARS-CoV-2 mutations emerging in variants of concern. After virus infection, host ER resident sigma receptors form direct interactions with non-structural SARS-CoV-2 proteins present in the replication complex. (2) Methods: In this work, highly specific sigma receptor ligands were investigated for their ability to inhibit both SARS-CoV-2 genome replication and virus induced cellular toxicity. This study found antiviral activity associated with agonism of the sigma-1 receptor (e.
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23

Maron, Michael B., and Cahrles F. Pilati. "Effect of papaverine on pulmonary vascular permeability to proteins." Journal of Applied Physiology 66, no. 6 (1989): 2919. http://dx.doi.org/10.1152/jappl.1989.66.6.2919-s.

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Page 1367–1371: Michael B. Maron and Charles F. Pilati. “Effect of papaverine on pulmonary vascular permeability to proteins.” Page 1370: right-hand column, sentence beginning on line 23 should read: For example, we previously found that histamine does not decrease sigma in this preparation, although we found it to do so in an isolated perfused canine forelimb preparation (11).
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Maron, M. B., and C. F. Pilati. "Effect of papaverine on pulmonary vascular permeability to proteins." Journal of Applied Physiology 65, no. 3 (1988): 1367–71. http://dx.doi.org/10.1152/jappl.1988.65.3.1367.

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Previous studies have suggested that papaverine, a drug commonly used in studies of transvascular fluid and solute exchange to eliminate confounding effects of changes in vascular tone, may itself increase vascular permeability. In this study, we determined the ability of papaverine to alter pulmonary vascular protein permeability by measuring the osmotic reflection coefficient (sigma) for total proteins in a canine isolated perfused left lower lung lobe (LLL) preparation. The reflection coefficient, determined by the hematocrit-protein double-indicator technique, for control LLL's was 0.83 +/
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Shadel, G. S., and D. A. Clayton. "A Saccharomyces cerevisiae mitochondrial transcription factor, sc-mtTFB, shares features with sigma factors but is functionally distinct." Molecular and Cellular Biology 15, no. 4 (1995): 2101–8. http://dx.doi.org/10.1128/mcb.15.4.2101.

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In Saccharomyces cerevisiae mitochondria, sc-mtTFB is a 341-amino-acid transcription factor required for initiation of transcription from mitochondrial DNA promoters. Specific transcription in vitro requires only sc-mtTFB and the bacteriophage-related core sc-mtRNA polymerase. Mutational analysis of sc-mtTFB has defined two regions of the protein that are important for normal function both in vivo and in vitro. These regions overlap portions of the protein that exhibit similarity to conserved region 2 of bacterial sigma factors. One mutation in this region of sc-mtTFB (tyrosine 108 to arginine
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Lee, Jong-Hee, Petros C. Karakousis, and William R. Bishai. "Roles of SigB and SigF in the Mycobacterium tuberculosis Sigma Factor Network." Journal of Bacteriology 190, no. 2 (2007): 699–707. http://dx.doi.org/10.1128/jb.01273-07.

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ABSTRACTTo characterize the roles of SigB and SigF in sigma factor regulation inMycobacterium tuberculosis, we used chemically inducible recombinant strains to conditionally overexpresssigBandsigF.Using whole genomic microarray analysis and quantitative reverse transcription-PCR, we investigated the resulting global transcriptional changes aftersigBinduction, and we specifically tested the relative expression of other sigma factor genes after knock-in expression ofsigBandsigF. Overexpression ofsigBresulted in significant upregulation of genes encoding several early culture filtrate antigens (E
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Babu, Suganthan Rajan, Yumiko Sato, Shoji Yagi, Gyedu Ampaabeng, and Nobuo Shimamoto. "1P208 Possible contradiction of the consensus mechanism of E. coli sigma-70 and the observed activity of its N- terminus half proteins(7. Nucleic acid binding protein,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)." Seibutsu Butsuri 46, supplement2 (2006): S198. http://dx.doi.org/10.2142/biophys.46.s198_4.

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Sexton, W. L., R. J. Korthuis, and M. H. Laughlin. "Ischemia-reperfusion injury in isolated rat hindquarters." Journal of Applied Physiology 68, no. 1 (1990): 387–92. http://dx.doi.org/10.1152/jappl.1990.68.1.387.

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The purpose of this study was to determine the suitability of the maximally vasodilated (papaverine) isolated rat hindquarters preparation to study the effects of ischemia and reperfusion on the microvasculature of skeletal muscle. The osmotic reflection coefficient for plasma proteins (sigma) and total vascular resistance (RT, mmHg.ml-1.min.100 g-1) were determined before ischemic periods of 30, 60, 120, 180, and 240 min in intact (with skin) and 30, 60, and 120 min in skinned hindquarters and again after 60 min of reperfusion. In both intact and skinned hindquarters, reductions in sigma and
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Bosso, F. J., M. B. Maron, C. F. Pilati, and D. G. Jarjoura. "Pulmonary vascular protein sieving capability after exposure to high vascular pressures." Journal of Applied Physiology 73, no. 1 (1992): 50–58. http://dx.doi.org/10.1152/jappl.1992.73.1.50.

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We evaluated the ability of the canine in situ left lower lobe (LLL) vasculature to sieve endogenous plasma proteins of various molecular radii (34–124 A) after LLL arterial pressure had been transiently elevated to 23.8 +/- 0.9 (control group, n = 5) or 92.3 +/- 1.4 (SE) Torr (high-pressure group, n = 9) by restricting LLL venous outflow under conditions of constant flow. After LLL flow was returned to natural perfusion, left atrial pressure was elevated in step increments, and LLL lymph and blood samples were collected until filtration-independent lymph-to-plasma protein concentration ratios
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Sipahutar, Rizki Ananda, and Ismail. "Analisis Pengendalian Kualitas Produk Kemasan Makanan Ternak Dengan Metode Six Sigma Dan Analisa Kaizen di PT. Central Proteina Prima Tbk." Journal Technology and Industrial Engineering (JTIE) 1, no. 2 (2023): 153–61. http://dx.doi.org/10.59840/jtie.v1i2.170.

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Guna menjaga image tersebut, pastinya industri ini senantiasa berinovasi dalam perihal kenaikan mutu produk yang bertujuan buat tingkatkan performansi industri dengan menggapai zero defect. Metode Six Sigma merupakan konsep statistik yang mengukur sebuah proses yang berkaitan dengan cacat ataupun kehancuran. Metode ini dinilai selaku suatu metode yang sanggup melaksanakan pendekatan secara komprehensif, yang secara dramatis hendak tingkatkan mutu dari produk yang dihasilkan. Bersumber pada hasil pengolahan informasi dengan metode Six Sigma, jumlah penciptaan 140, 330 jumlah cacat penciptaan te
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Parashar, Archana, Kimberley R. Colvin, Dawn R. D. Bignell, and Brenda K. Leskiw. "BldG and SCO3548 Interact Antagonistically To Control Key Developmental Processes in Streptomyces coelicolor." Journal of Bacteriology 191, no. 8 (2009): 2541–50. http://dx.doi.org/10.1128/jb.01695-08.

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ABSTRACT The similarity of BldG and the downstream coexpressed protein SCO3548 to anti-anti-sigma and anti-sigma factors, respectively, together with the phenotype of a bldG mutant, suggests that BldG and SCO3548 interact as part of a regulatory system to control both antibiotic production and morphological differentiation in Streptomyces coelicolor. A combination of bacterial two-hybrid, affinity purification, and far-Western analyses demonstrated that there was self-interaction of both BldG and SCO3548, as well as a direct interaction between the two proteins. Furthermore, a genetic compleme
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Laborenz, Janina, Yury S. Bykov, Katharina Knöringer, et al. "The ER protein Ema19 facilitates the degradation of nonimported mitochondrial precursor proteins." Molecular Biology of the Cell 32, no. 8 (2021): 664–74. http://dx.doi.org/10.1091/mbc.e20-11-0748.

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Ema19 is an integral endoplasmic reticulum (ER) protein with relevance for mitochondrial biogenesis. This yeast, representative of the TMEM97/sigma 2 receptor family, serves as quality control factor which supports the degradation of nonimported mitochondrial proteins. This study again demonstrates the important role of the ER in mitochondrial protein targeting.
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Pampura, A. N., E. F. Zhukalina, M. A. Morenko, and O. P. Usenova. "Modern approaches to the diagnosis and management of children with allergy to cow’s milk proteins." Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics) 68, no. 2 (2023): 39–46. http://dx.doi.org/10.21508/1027-4065-2023-68-2-39-46.

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Allergy to cow’s milk proteins is the most common cause of allergic reactions in young children, with a significant impact on the quality of life of children and their families. The most significant biomarker of herbivore milk allergy is allergen-specific IgE (sIgE), which can be assessed both for the whole allergen (for example, cow’s milk (CM), mare’s milk, goat’s milk, etc.) and a specific molecule, included in their composition. This article focuses on the use of sIgE in infants with suspected cow’s milk protein allergy.
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Jiang, Yuan-Hong, Jia-Fong Jhang, Lori A. Birder, and Hann-Chorng Kuo. "Sensory Receptor, Inflammatory, and Apoptotic Protein Expression in the Bladder Urothelium of Patients with Different Subtypes of Interstitial Cystitis/Bladder Pain Syndrome." International Journal of Molecular Sciences 24, no. 1 (2023): 820. http://dx.doi.org/10.3390/ijms24010820.

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The aim of this study was to investigate the expression levels of sensory receptors, inflammatory proteins, and pro-apoptotic proteins in the urothelium of non-Hunner’s interstitial cystitis (NHIC) bladders of patients with different clinical and cystoscopic phenotypes. The urothelia from the bladders of 52 NHIC patients were harvested. The expression of sensory receptors, including TRPV1, TRPV4, TRPA1, H1-receptors, and sigma-1 receptors; the inflammatory proteins p38 and tryptase; and the pro-apoptotic proteins, such as caspase-3, BAD, and BAX in the urothelium, were investigated using immun
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35

Min, K. T., and M. D. Yudkin. "Activity of mutant sigma F proteins truncated near the C terminus." Journal of Bacteriology 174, no. 22 (1992): 7144–48. http://dx.doi.org/10.1128/jb.174.22.7144-7148.1992.

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Miyazaki, Eishi, Jong-Min Chen, Chiew Ko, and William R. Bishai. "The Staphylococcus aureus rsbW(orf159) Gene Encodes an Anti-Sigma Factor of SigB." Journal of Bacteriology 181, no. 9 (1999): 2846–51. http://dx.doi.org/10.1128/jb.181.9.2846-2851.1999.

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ABSTRACT SigB, a newly discovered alternative sigma factor ofStaphylococcus aureus, has been shown to play an important role in stress responses and the regulation of virulence factors. ThersbW (orf159) gene is immediately upstream ofsigB. Its gene product is homologous to Bacillus subtilis RsbW which under appropriate conditions binds toB. subtilis SigB and functions as an anti-sigma factor or negative posttranslational regulator. To define the function ofS. aureus RsbW, both the S. aureus SigB and RsbW proteins were expressed in Escherichia coli and purified. Cross-linking experiments with t
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37

Martínez-Salazar, Jaime M., Emmanuel Salazar, Sergio Encarnación, Miguel A. Ramírez-Romero, and Javier Rivera. "Role of the Extracytoplasmic Function Sigma Factor RpoE4 in Oxidative and Osmotic Stress Responses in Rhizobium etli." Journal of Bacteriology 191, no. 13 (2009): 4122–32. http://dx.doi.org/10.1128/jb.01626-08.

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ABSTRACT The aims of this study were to functionally characterize and analyze the transcriptional regulation and transcriptome of the Rhizobium etli rpoE4 gene. An R. etli rpoE4 mutant was sensitive to oxidative, saline, and osmotic stresses. Using transcriptional fusions, we determined that RpoE4 controls its own transcription and that it is negatively regulated by rseF (regulator of sigma rpoE4; CH03274), which is cotranscribed with rpoE4. rpoE4 expression was induced not only after oxidative, saline, and osmotic shocks, but also under microaerobic and stationary-phase growth conditions. The
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38

Schiff, L. A., M. L. Nibert, M. S. Co, E. G. Brown, and B. N. Fields. "Distinct binding sites for zinc and double-stranded RNA in the reovirus outer capsid protein sigma 3." Molecular and Cellular Biology 8, no. 1 (1988): 273–83. http://dx.doi.org/10.1128/mcb.8.1.273-283.1988.

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By atomic absorption analysis, we determined that the reovirus outer capsid protein sigma 3, which binds double-stranded RNA (dsRNA), is a zinc metalloprotein. Using Northwestern blots and a novel zinc blotting technique, we localized the zinc- and dsRNA-binding activities of sigma 3 to distinct V8 protease-generated fragments. Zinc-binding activity was contained within an amino-terminal fragment that contained a transcription factor IIIA-like zinc-binding sequence, and dsRNA-binding activity was associated with a carboxy-terminal fragment. By these techniques, new zinc- and dsRNA-binding acti
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39

Schiff, L. A., M. L. Nibert, M. S. Co, E. G. Brown, and B. N. Fields. "Distinct binding sites for zinc and double-stranded RNA in the reovirus outer capsid protein sigma 3." Molecular and Cellular Biology 8, no. 1 (1988): 273–83. http://dx.doi.org/10.1128/mcb.8.1.273.

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By atomic absorption analysis, we determined that the reovirus outer capsid protein sigma 3, which binds double-stranded RNA (dsRNA), is a zinc metalloprotein. Using Northwestern blots and a novel zinc blotting technique, we localized the zinc- and dsRNA-binding activities of sigma 3 to distinct V8 protease-generated fragments. Zinc-binding activity was contained within an amino-terminal fragment that contained a transcription factor IIIA-like zinc-binding sequence, and dsRNA-binding activity was associated with a carboxy-terminal fragment. By these techniques, new zinc- and dsRNA-binding acti
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40

Rippe, B., M. Townsley, J. C. Parker, and A. E. Taylor. "Osmotic reflection coefficient for total plasma protein in lung microvessels." Journal of Applied Physiology 58, no. 2 (1985): 436–42. http://dx.doi.org/10.1152/jappl.1985.58.2.436.

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The osmotic reflection coefficient (sigma) for total plasma proteins was estimated in 11 isolated blood-perfused canine lungs. Sigma's were determined by first measuring the capillary filtration coefficient (Kf,C in ml X min-1 X 100g-1 X cmH2O-1) using increased hydrostatic pressures and time 0 extrapolation of the slope of the weight gain curve. Kf,C averaged 0.19 +/- 0.05 (mean +/- SD) for 14 separate determinations in the 11 lungs. Following a Kf,C determination, the isogravimetric capillary pressure (Pc,i) was determined and averaged 9.9 +/- 0.5 cmH2O for all controls reported in this stud
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41

Miller, Eric S., Elizabeth Kutter, Gisela Mosig, Fumio Arisaka, Takashi Kunisawa, and Wolfgang Rüger. "Bacteriophage T4 Genome." Microbiology and Molecular Biology Reviews 67, no. 1 (2003): 86–156. http://dx.doi.org/10.1128/mmbr.67.1.86-156.2003.

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SUMMARY Phage T4 has provided countless contributions to the paradigms of genetics and biochemistry. Its complete genome sequence of 168,903 bp encodes about 300 gene products. T4 biology and its genomic sequence provide the best-understood model for modern functional genomics and proteomics. Variations on gene expression, including overlapping genes, internal translation initiation, spliced genes, translational bypassing, and RNA processing, alert us to the caveats of purely computational methods. The T4 transcriptional pattern reflects its dependence on the host RNA polymerase and the use of
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42

Reed, R. K., M. I. Townsley, and A. E. Taylor. "Estimation of capillary reflection coefficients and unique PS products in dog paw." American Journal of Physiology-Heart and Circulatory Physiology 257, no. 3 (1989): H1037—H1041. http://dx.doi.org/10.1152/ajpheart.1989.257.3.h1037.

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Lymphatic protein flux (Js) obtained in canine hindpaws at low lymph flows were used to determine the capillary osmotic reflection coefficients (sigma d) and unique permeability surface area (PS) products for total proteins, albumin, immunoglobulin (Ig)G, and IgM. This new analysis is based on the phenomenon that when maximal diffusion occurs across the capillary membrane, the Peclet number [x = Jv(1 - sigma d)/PS] attains a unique value defined only by sigma d. The diffusive flux is maximal when the relationship between protein flux and transcapillary fluid flux (Jv) changes from a curvilinea
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43

Paul, Deborupa, and Sanmitra Ghosh. "An overview of heat-stress response regulation in Gram-negative bacteria considering Escherichia coli as a model organism." Journal of Experimental Biology and Agricultural Sciences 10, no. 1 (2022): 190–200. http://dx.doi.org/10.18006/2022.10(1).190.200.

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Response to heat stress (HSR) is a key stress response for endurance in Escherichia coli mediated by transcriptional factor σ-32. Even though there has been extensive investigation on the contribution of proteins and chaperones in retaining protein stability in cells under stress conditions, limited information is available regarding the dynamic nature of mechanisms regulating the activity of the highly conserved heat shock proteins (Hsps). Several gene expression-based studies suggest the pivotal role of Hsp70 (DnaK) in the regulation of the expression of heat shock genes (Hsg). Direct intera
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44

Ehrhart, I. C., and W. F. Hofman. "Pressure-dependent increase in lung vascular permeability to water but not protein." Journal of Applied Physiology 72, no. 1 (1992): 211–18. http://dx.doi.org/10.1152/jappl.1992.72.1.211.

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Simultaneous measures of vascular permeability to fluid (capillary filtration coefficient, Kf) and to plasma proteins (solvent drag reflection coefficient, sigma) were obtained over venous pressures (Pv) from 14 to 105 Torr in the isolated ventilated canine lung lobe (n = 70) pump perfused with autologous blood. The sigma was obtained from the relative increase in the concentration of plasma proteins vs. erythrocytes during fluid filtration. Kf's were obtained from two gravimetric methods as well as from change in hematocrit. All Kf's increased (P less than 0.05) as Pv was increased. However,
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45

Gordon, Nadria D., Geri L. Ottaviano, Sarah E. Connell та ін. "Secreted-Protein Response to σU Activity in Streptomyces coelicolor". Journal of Bacteriology 190, № 3 (2007): 894–904. http://dx.doi.org/10.1128/jb.01759-07.

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ABSTRACT The filamentous bacterium Streptomyces coelicolor forms an aerial mycelium as a prerequisite to sporulation, which occurs in the aerial hyphae. Uncontrolled activity of the extracytoplasmic function sigma factor σU blocks the process of aerial mycelium formation in this organism. Using a green fluorescent protein transcriptional reporter, we have demonstrated that sigU transcription is autoregulated. We have defined a σU-dependent promoter sequence and used this to identify 22 likely σU regulon members in the S. coelicolor genome. Since many of these genes encode probable secreted pro
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46

Wolf, M. B., P. D. Watson, and D. R. Scott. "Integral-mass balance method for determination of solvent drag reflection coefficient." American Journal of Physiology-Heart and Circulatory Physiology 253, no. 1 (1987): H194—H204. http://dx.doi.org/10.1152/ajpheart.1987.253.1.h194.

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We have developed the integral-mass balance (IMB) method to measure the solvent drag reflection coefficient (sigma f) for transcapillary macromolecular transport in skeletal muscle and other organs. Of course, sigma f is calculated from the cumulative amounts of water and macromolecule that move convectively across the microvascular membrane as determined from changes in hematocrit and plasma macromolecule concentration over a period of fluid filtration. We have investigated the effects of both theoretical and experimental factors that affect the validity and accuracy of the method. The effect
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47

Hahn, Mi-Young, Sahadevan Raman, Mauricio Anaya, and Robert N. Husson. "The Mycobacterium tuberculosis Extracytoplasmic-Function Sigma Factor SigL Regulates Polyketide Synthases and Secreted or Membrane Proteins and Is Required for Virulence." Journal of Bacteriology 187, no. 20 (2005): 7062–71. http://dx.doi.org/10.1128/jb.187.20.7062-7071.2005.

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ABSTRACT Mycobacterium tuberculosis sigL encodes an extracytoplasmic function (ECF) sigma factor and is adjacent to a gene for a membrane protein (Rv0736) that contains a conserved HXXXCXXC sequence. This motif is found in anti-sigma factors that regulate several ECF sigma factors, including those that control oxidative stress responses. In this work, SigL and Rv0736 were found to be cotranscribed, and the intracellular domain of Rv0736 was shown to interact specifically with SigL, suggesting that Rv0736 may encode an anti-sigma factor of SigL. An M. tuberculosis sigL mutant was not more susce
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Mathews, Sarah A., and Peter Timms. "Identification and Mapping of Sigma-54 Promoters inChlamydia trachomatis." Journal of Bacteriology 182, no. 21 (2000): 6239–42. http://dx.doi.org/10.1128/jb.182.21.6239-6242.2000.

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ABSTRACT The first ς54 promoters in Chlamydia trachomatis L2 were mapped upstream of hypothetical proteins CT652.1 and CT683. Comparative genomics indicated that these ς54 promoters and potential upstream activation binding sites are conserved in orthologous C. trachomatis D,C. trachomatis mouse pneumonitis strain, andChlamydia pneumoniae (CWL029 and AR39) genes.
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Reed, R. K., M. I. Townsley, R. J. Korthuis, and A. E. Taylor. "Analysis of lymphatic protein flux data. V. Unique PS products and sigma dS at low lymph flows." American Journal of Physiology-Heart and Circulatory Physiology 261, no. 3 (1991): H728—H740. http://dx.doi.org/10.1152/ajpheart.1991.261.3.h728.

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The selectivity of the capillary membrane to protein (osmotic reflection coefficient, sigma d) can be measured at high transcapillary volume flow when the capillary membrane can be considered as a true sieve. However, the diffusive capacity of the membrane (permeability-surface area product, PS) for macromolecules has not been directly measured, only estimated by assuming that transcapillary volume flow was zero. Based on unique properties of the Peclet number, a parameter that describes the ratio of solute convective flux relative to diffusive capacity, we have developed three new techniques
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Malhotra, Sonal, Laura A. Silo-Suh, Kalai Mathee, and Dennis E. Ohman. "Proteome Analysis of the Effect of Mucoid Conversion on Global Protein Expression in Pseudomonas aeruginosa Strain PAO1 Shows Induction of the Disulfide Bond Isomerase, DsbA." Journal of Bacteriology 182, no. 24 (2000): 6999–7006. http://dx.doi.org/10.1128/jb.182.24.6999-7006.2000.

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ABSTRACT Pseudomonas aeruginosa strains that cause chronic pulmonary infections in cystic fibrosis patients typically undergo mucoid conversion. The mucoid phenotype indicates alginate overproduction and is often due to defects in MucA, an antisigma factor that controls the activity of sigma-22 (AlgT [also called AlgU]), which is required for the activation of genes for alginate biosynthesis. In this study we hypothesized that mucoid conversion may be part of a larger response that activates genes other than those for alginate synthesis. To address this, a two-dimensional (2-D) gel analysis wa
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