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Artykuły w czasopismach na temat „Skin irritation test”

Autor: Grafiati

Data publikacji: 2 czerwca 2025

Data aktualizacji: 31 lipca 2025

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1

Bunga Eloystina, Laurencia Julia, Silvy Aldila, Valentina Girsang, and Anifatus Sa'adah. "Irritation Test of Optimum Preparation of Celery Extract Face Clay Cleanser With Sodium Lauryl Sulfate (SLS) and Cocamidopropyl Betaine (CAPB) as Surfactants." Science and Community Pharmacy Journal 3, no. 2 (2024): 211–16. https://doi.org/10.63520/scpj.v3i2.580.

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Surfactant is one of the important things in making face clay cleanser preparations. Sodium Lauryl Sulfate (SLS) is an anionic type surfactant that is often used in cosmetic products. The use of Sodium Lauryl Sulfate (SLS) can cause irritation and dry skin. Combination with cocamidopropyl betaine (CAPB) which is amophoteric can reduce skin irritation. Based on this, a safety test is needed which aims to meet the preparation requirements before being marketed to the wider community. One of the safety tests that can be done is the skin irritation test. The test method used is the human patch tes

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Deswani, Deswani, Sri Djuwitaningsih, Harpolia Cartika, and Paul Malau. "Irritation Test of Cream Preparations Made from Purple Cabbage Extract Against Healthy Skin Using The Path Test Method." JURNAL INFO KESEHATAN 23, no. 1 (2025): 1–9. https://doi.org/10.31965/infokes.vol23.iss1.1458.

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Testing for skin irritation caused by new substances is a critical component of safety evaluation procedures. Pharmaceutical formulations and active ingredients intended for topical application have the potential to cause skin irritation, necessitating thorough safety assessments prior to public distribution. This study aimed to evaluate the primary irritation potential of a purple cabbage extract cream using the patch test method on healthy older adults. The experimental research involved 18 volunteers, who were administered 0.625% purple cabbage extract cream and a placebo preparation withou

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Deswani, Deswani, Sri Djuwitaningsih, and Harpolia Cartika. "Irritation Test of Cream Preparations Made from Purple Cabbage Extract Against Healthy Skin Using The Path Test Method." JURNAL INFO KESEHATAN 22, no. 4 (2024): 858–66. https://doi.org/10.31965/infokes.vol22.iss4.1458.

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Testing for skin irritation caused by new substances is a critical component of safety evaluation procedures. Pharmaceutical formulations and active ingredients intended for topical application have the potential to cause skin irritation, necessitating thorough safety assessments prior to public distribution. This study aimed to evaluate the primary irritation potential of a purple cabbage extract cream using the patch test method on healthy older adults. The experimental research involved 18 volunteers, who were administered 0.625% purple cabbage extract cream and a placebo preparation withou

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Kandárová, Helena, Patrick Hayden, Erin Spiller, Mitch Klausner, Joseph Kubilus, and John Sheasgreen. "In vitro skin irritation test: Increasing the sensitivity of the EpiDerm skin irritation protocol evaluated in the ECVAM skin irritation validation study." Toxicology Letters 172 (October 2007): S81. http://dx.doi.org/10.1016/j.toxlet.2007.05.230.

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Draelos, Zoe, Emil Tanghetti, Linda Stein Gold, et al. "Dermal Irritation, Sensitization, and Safety of Fixed-Dose Triple-Combination Clindamycin Phosphate 1.2%/Benzoyl Peroxide 3.1%/Adapalene 0.15% Gel in Healthy Participants." SKIN The Journal of Cutaneous Medicine 7, no. 4 (2023): s227. http://dx.doi.org/10.25251/skin.7.supp.227.

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Introduction: IDP-126 polymeric mesh gel (clindamycin phosphate 1.2%/benzoyl peroxide [BPO] 3.1%/adapalene 0.15%) is the first triple-combination, fixed-dose topical acne product in development and it addresses major acne pathophysiological processes. IDP-126 demonstrated superior efficacy to vehicle and component dyads, with good safety/tolerability in a phase 2 study of participants with moderate-to-severe acne. The objectives of this study were to assess dermal irritation/sensitization and safety of IDP-126 gel in two phase 1 studies and to compare irritancy of IDP-126 gel and commercially

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Amasa, Wayessa, Dante Santiago, Seblework Mekonen, and Argaw Ambelu. "Are Cosmetics Used in Developing Countries Safe? Use and Dermal Irritation of Body Care Products in Jimma Town, Southwestern Ethiopia." Journal of Toxicology 2012 (2012): 1–8. http://dx.doi.org/10.1155/2012/204830.

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Background. Rabbit skin model was used to test skin irritation of the most commonly used cosmetic products in Jimma town, southwestern Ethiopia. The most commonly used cosmetics were Dove, Glysolid, College, Top Society, Fair and Lovely, Nivea, Lux, Magic fruit world, Solea, Body talk, Kris, Holly, Victoria, and Sweet Heart.Methods. Intact and abraded rabbit skins were tested for erythema and edema under shade and under sun exposure. Draize Primary Irritation Index (PII) was used to calculate skin irritation of each cosmetic. Cosmetic ingredients were analyzed from the labels.Results and Discu

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Suhendriyo, Suhendriyo, Dwi Astuti Handayani, and Susilo Yulianto. "Test Physical and Test Irritation Provision Cream Extract Aromatic Ginger ( Kaempferia Galanga L.)." ULIL ALBAB : Jurnal Ilmiah Multidisiplin 4, no. 4 (2025): 1192–99. https://doi.org/10.56799/jim.v4i4.8232.

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Galangal rhizome is used in traditional medicine which has effectiveness as an anti-inflammatory and antitoxin. Galangal rhizome contains flavonoids, polyphenols, saponins, essential oils. The content of polyphenols in the form of ethylcinnamate can provide anti-inflammatory effects. The content of saponins, flavonoids, essential oils in galangal rhizome as a treatment for mycosis. The purpose of this study was to determine the results of physical tests and irritation tests of galangal extract cream preparations including organoleptic tests, homogeneity tests, spreadability tests, adhesion tes

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Banerjee, Subham, Pronobesh Chattopadhyay, Animesh Ghosh, Manash Pratim Pathak, Shweta Singh, and Vijay Veer. "Acute Dermal Irritation, Sensitization, and Acute Toxicity Studies of a Transdermal Patch for Prophylaxis Against (±) Anatoxin-A Poisoning." International Journal of Toxicology 32, no. 4 (2013): 308–13. http://dx.doi.org/10.1177/1091581813489996.

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The skin irritating, sensitizing, and acute dermal toxicity potential of a novel combinational prophylactic transdermal patch, mainly composed of eserine and pralidoxime chloride as active pharmaceutical ingredients, against (±) anatoxin-a poisoning were investigated in rabbits, guinea pigs, and rats in compliance with the Organisation for Economic Cooperation and Development guidelines. In primary skin irritation test, rabbits were dermally attached with the therapeutically active transdermal patch or with a placebo patch for 72 hours. The transdermal patches did not induce any adverse reacti

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Basketter, David, Dagmar Jírova, and Helena Kandárová. "Review of skin irritation/corrosion hazards on the basis of human data: a regulatory perspective." Interdisciplinary Toxicology 5, no. 2 (2012): 98–104. http://dx.doi.org/10.2478/v10102-012-0017-2.

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Abstract Regulatory classification of skin irritation has historically been based on rabbit data, however current toxicology processes are transitioning to in vitro alternatives. The in vitro assays have to provide sufficient level of sensitivity as well as specificity to be accepted as replacement methods for the existing in vivo assays. This is usually achieved by comparing the in vitro results to classifications obtained in animals. Significant drawback of this approach is that neither in vivo nor in vitro methods are calibrated against human hazard data and results obtained in these assays

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Gfeller, W., W. Kobel, and G. Seifert. "Overview of animal test methods for skin irritation." Food and Chemical Toxicology 23, no. 2 (1985): 165–68. http://dx.doi.org/10.1016/0278-6915(85)90009-2.

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Ghazali, Ahmad Rohi, Nor Fadilah Rajab, Muhammad Firas Zainuddin, and Nazeha Ahmat. "Assessment of Skin Irritation and Sensitisation Effects by Topical Pterostilbene." Biomedical and Pharmacology Journal 14, no. 4 (2021): 1917–27. http://dx.doi.org/10.13005/bpj/2290.

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Pterostilbene has dermal medicinal benefits such as anti-inflammatory, antioxidative effects and photoprotective properties against UVB radiation. The purpose of this study was to evaluate the dermal toxicity of pterostilbene via skin irritation and sensitisation. A skin irritation test was done according to the Organization Economic Co-operation and Development 404 guideline with the scoring of irritation based on erythema and oedema in 5 albino rabbits were observed up to 14 days. The sensitisation test using the Buehler Test in accordance with the ISO 10993-10 guideline was used to study th

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12

S, Panji Ratih, Sih Wahyu R, Erna Fitriyany, Cikra I, Finna Calya, and Septiana Sari. "PEMANFAATAN LIMBAH KULIT BAWANG PUTIH (Allium sativum L.) SEBAGAI BAHAN AKTIF PEMBUATAN SABUN CAIR." Jurnal Riset Kefarmasian Indonesia 5, no. 3 (2023): 427–38. http://dx.doi.org/10.33759/jrki.v5i3.392.

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Utilization of household waste in Indonesia is still very rarely done, one of which is the use of garlic skin waste. From research by Allemann & Baumann in 2008, garlic skin is rich in antioxidant compounds so it can protect skin cells from free radical damage if used as a skincare product, one of which is liquid bath soap for the body. This research aims to formulate garlic skin extract as liquid soap. The concentration used is 10%. The liquid soap formulation used was 17 g of SLS, 3 g of NaCl, 9 g of Cocamidopropyl Betaine, 9 g of Glycerin, Fragrances, and Dyes to taste. Garlic skin extr

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Botham, Philip A., Lesley K. Earl, Julia H. Fentem, Roland Roguet, and Johannes J. M. van de Sandt. "Alternative Methods for Skin Irritation Testing: The Current Status." Alternatives to Laboratory Animals 26, no. 2 (1998): 195–211. http://dx.doi.org/10.1177/026119299802600205.

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The ECVAM Skin Irritation Task Force was established in November 1996, primarily to prepare a report on the current status of the development and validation of alternative tests for skin irritation and corrosion and, in particular, to identify any appropriate non-animal tests for predicting human skin irritation which were sufficiently well-developed to warrant ECVAM supporting their prevalidation/validation. The task force based its discussions around the proposed testing strategy for skin irritation/corrosion emanating from an OECD workshop held in January 1996. The following have been revie

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14

SoonOk, Woo,, Sangmi Han, Inpyo Hong, and Kim, Sung-kuk. "In vitro Skin Irritation Test of Honeypolis using Human Skin Model." Journal of Apiculture 33, no. 4 (2018): 277–82. http://dx.doi.org/10.17519/apiculture.2018.11.33.4.277.

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Novasari, Hastrina, Asti Pratiwi, and Nopri Mahmiara. "EFEKTIVITAS FORMULASI SEDIAAN LOTION DARI EKSTRAK ETANOL DAUN ALPUKAT (PERSEA AMERICANA MILL) SEBAGAI PELEMBAB KULIT." JURNAL FARMASIMED (JFM) 3, no. 2 (2021): 57–62. http://dx.doi.org/10.35451/jfm.v3i2.571.

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Avocado plant can be formulated into a lotion because preparation based on previous research avocado leavescontain high antioxidant is represented with flavonoid compound. The purpose of this study is to know avocado leaves can be formulated into the preparation of lotion and to know the preparation of lotion from extract etanol avocado leavescan moisturaize the skin. The research uses exsperimental method. Avocado leaves is exstrach by maceration process. Exanination of lotion preparation by this research is phytochemical screening test, pH test,irritation test and skin moisturaise test

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16

Han, Sang Mi, Kwang Gill Lee, Joo Hong Yeo, and Sok Cheon Pak. "Dermal and Ocular Irritation Studies of Honeybee (Apis mellifera L.) Venom." American Journal of Chinese Medicine 40, no. 04 (2012): 795–800. http://dx.doi.org/10.1142/s0192415x12500590.

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The aim of this study was to access the irritant properties of bee venom (BV) after its application to skin and eye mucous membranes of the rabbit. The animals were also observed for clinical signs and mortality after the application of the test material. Six animals were used for the skin irritation test and nine rabbits for the eye irritation test. The acute BV application to the rabbit skin revealed no appreciable clinical signs throughout the observation period of 72 h and there was no mortality seen. In the eye irritation test, eye reactions were read and graded 24, 48, 72, 96 and 168 h a

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Tama Octi R. Ramli and Mazaya Fadhila. "Uji Iritasi Gel Ekstrak Etanol Herba Pegagan (Centella Asiatica L ) Dengan Gelling Agent Carbopol 940." Journal Pharma Saintika 6, no. 1 (2022): 08–15. http://dx.doi.org/10.51225/jps.v6i1.16.

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Gel is a form of cosmetics, before being marketed cosmetics must pass a safety test. The safety test can be carried out using the patch test method, in which the test substance is attached to normal skin, then covered and carried out for 24 hours. Herb gotu kola has the property of accelerating wound healing. Centella asiatica herb extract was prepared by maceration method with 96% ethanol as a solvent and formulated in gel form using carbopol as a gelling agent. The preparations were made into 2 formulas with a ratio of formula 1 without extract and formula 2 using 12.5 mg extract. The irrita

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Cruzan, George, Walden E. Dalbey, Christopher J. D'Aleo, and Edward J. Singer. "A Composite Model for Multiple Assays of Skin Irritation." Toxicology and Industrial Health 2, no. 3 (1986): 309–20. http://dx.doi.org/10.1177/074823378600200309.

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A model for skin irritation was developed for simultaneous evalua tion of the influence on irritation of abrasion, occlusion, and dura tion of treatment and for fulfillment of requirements for labeling considerations under DOT, CPSC-FHSA, OSHA, and EEC. This model greatly reduces the number of animals required to address submissions under multiple agencies compared to performing each test separately. In this model, which we have called a Composite Skin Irritation test, a test material is placed on three pairs of intact and abraded sites on each rabbit; one pair of sites is occluded for 4 hours

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Rahmi, Dwinna, Retno Yunilawati, Ira Setiawati, et al. "ANTIOXIDANT ACTIVITY, SKIN IRRITAION POTENTIAL AND CHEMICAL COMPOSITION OF CLOVE LEAF OIL FROM WEST JAVA INDONESIA." Jurnal Sains Materi Indonesia 23, no. 1 (2021): 24. http://dx.doi.org/10.17146/jsmi.2021.23.1.6398.

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ANTIOXIDANT ACTIVITY, SKIN IRRITATON POTENTIAL, AND CHEMICAL COMPOSITION OF CLOVE LEAF OIL FROM WEST JAVA INDONESIA. Essential oils with specific chemical compositions have the potential as a source of antioxidants in cosmetics, however, in general, essential oils are not safe because of the potential for skin irritation. This study aims is to perform fractionation to obtain clove oil with a chemical composition that is safe on the skin and does not reduce its antioxidant activity. Fractionation was performed at a temperature of 100 – 285 oC. Chemical composition was determined by Mass Chromat

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Situmorang, Easter C. H., Robert A. Bara, Veibe Warouw, et al. "Anti UV, the Sun Protection Factor (SPF), and Irritation Test of Coastal Vegetation Ricinus communis." BIO Web of Conferences 92 (2024): 02017. http://dx.doi.org/10.1051/bioconf/20249202017.

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This study aims to test anti-UV activity, determine the SPF, and perform an irritation test on human skin from Ricinus communis leaf extract. The anti-UV and SPF values were determined using a UV-Vis Spectrophotometer. Furthermore, the irritation test was performed by observing the skin of human objects after cream with R. communis extract was applied. The results show that R. communis leaf extract has anti-UV-B activity, With SPF value categorized as high according to the US FDA at a concentration of 1000 ppm. Furthermore, observations from 20 participants from the patch test found no skin re

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Easter, C.H. Situmorang, A. Bara Robert, Warouw Veibe, et al. "Testing of UV Protection Activity, SPF Determination, and Irritation Assessment of Coastal Vegetation Ipomoea Pes-Caprae." INTERNATIONAL JOURNAL OF HEALTH & MEDICAL RESEARCH 03, no. 11 (2024): 805–10. https://doi.org/10.5281/zenodo.14192038.

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Total solar radiation contains approximately 10% ultraviolet light. Ultraviolet rays have benefits such as providing warmth, light, and helping to synthesise vitamin D3. However, ultraviolet rays can also have negative impacts on the skin such as browning, hyperpigmentation, erythema, and premature ageing. Natural sunscreens with high SPF are useful in reducing the negative effects of ultraviolet rays. This study aims to determine anti-UV activity, SPF value, and assessment of human skin irritation or allergy to sunscreen cream from Ipomoea pes-caprae leaves. Anti-UV activity test and SPF valu

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Walz, D. "Quantitative assessment of irritation in the mouse skin test." Food and Chemical Toxicology 23, no. 2 (1985): 199–203. http://dx.doi.org/10.1016/0278-6915(85)90017-1.

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Politano, Valerie T., Daniel A. Isola, Jon Lalko, and Anne Marie Api. "The Effects of Vehicles on the Human Dermal Irritation Potentials of Allyl Esters." International Journal of Toxicology 25, no. 3 (2006): 183–93. http://dx.doi.org/10.1080/10915810600683275.

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Allyl esters, frequently used in the fragrance industry, often contain a certain percentage of free allyl alcohol. Allyl alcohol is known to have a potential for delayed skin irritation. Also present in the finished product are different solvent systems, or vehicles, which are used to deliver the fragrances based upon their intended application. This study was conducted to determine whether different vehicles affect the skin irritation potential of five different allyl esters. The allyl esters tested were allyl amyl glycolate, allyl caproate, allyl (cyclohexyloxy)acetate, allyl cyclohexylpropi

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Kandárová, Helena, Patrick Hayden, Mitch Klausner, Joseph Kubilus, Paul Kearney, and John Sheasgreen. "In VitroSkin Irritation Testing: Improving the Sensitivity of the EpiDerm Skin Irritation Test Protocol." Alternatives to Laboratory Animals 37, no. 6 (2009): 671–89. http://dx.doi.org/10.1177/026119290903700611.

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Farage, Miranda A., and Howard Maibach. "Cumulative Skin Irritation Test of Sanitary Pads in Sensitive Skin and Normal Skin Population." Cutaneous and Ocular Toxicology 26, no. 1 (2007): 37–43. http://dx.doi.org/10.1080/15569520601183831.

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Maulina, Novia, Faisal Akhmal Muslikh, Ginanjar Putri Nastiti, et al. "Formulasi Sabun Mandi Cair Berbasis Virgin Coconut Oil (VCO): Optimalisasi Pemanfaatan Produk Lokal Untuk Perawatan Kulit dan Pencegahan Dehidrasi." Bioscientist : Jurnal Ilmiah Biologi 13, no. 1 (2025): 628. https://doi.org/10.33394/bioscientist.v13i1.14160.

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Skin dehydration is a common issue affecting 40–50% of the adult population, resulting in dry, rough, and dull skin, as well as itching, irritation, and premature aging. The "back to nature" trend has increased interest in natural skincare products that are more environmentally friendly and gentler on the skin. Virgin coconut oil (VCO) is a popular natural ingredient known for its ability to maintain skin moisture and provide protection. This study aims to develop a liquid soap formulation using VCO to address skin dehydration. The research methods included formulation and evaluation of liquid

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H.I., Muhammad Aiman, S. P. Koh, Y. S. Sew, et al. "Anti-inflammatory efficacy and skin irritation study of Cleome gynandra extract: an animal model and pre-clinical evaluation." Food Research 8, Supplementary 3 (2024): 92–103. http://dx.doi.org/10.26656/fr.2017.8(s3).14.

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Cleome gynandra, a kind of medicinal herb which has strong antioxidant properties has been traditionally used for treating various ailments including skin inflammatory diseases. To date, the anti-inflammatory efficacy of C. gynandra based cream has not been reported. The aim of the study was to evaluate the anti-inflammatory activities of C. gynandra leaf extract cream using an in vivo arachidonic acid-induced ear edema in the rat model. The expression of genes related to rat ear inflammation was measured to evaluate the topical anti-inflammatory activity of C. gynandra cream. The toxicity eff

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Winarti, Lina, Shifwatu Dzakkiyah, Eka Deddy Irawan, and Erlia Narulita. "Optimisation of seed oil and powder of Momordica charantia in the formulation of body scrub cream." Pharmacy Education 23, no. 4 (2023): 127–31. http://dx.doi.org/10.46542/pe.2023.234.127131.

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Background: Momordica charantia seed oil and powder are potential ingredients for a body scrub. The seeds contain fatty acids to moisturise the skin, and the mineral content to remove dead skin cells and nourish the skin. Objective: To determine the optimal efficiency of Momordica charantia seed oil and powder on body scrub cream produce. Methods: The body scrub used W/O (water-in-Oil) cream base. Simplex Lattice Design was used to determine five formulas. The irritation test with the Draize Skin Test method was carried out on rats (n = three, in each formula), and the Primary Dermal Irritatio

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Retnowati, Dini, Retno Sari, Esti Hendradi, and Septiani Septiani. "The stability and irritability study of the chitosan–Aloe vera spray gel as wound healing." Journal of Basic and Clinical Physiology and Pharmacology 32, no. 4 (2021): 651–56. http://dx.doi.org/10.1515/jbcpp-2020-0407.

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Abstract Objectives Chitosan is a natural polysaccharide widely used in various clinical applications including regeneration of skin tissue. Aloe vera has properties in healing burns on the skin, anti-inflammatory effect, and leaves a protective layer on the skin after drying so it provides protection to the wound. The spray gel of chitosan–A. vera was developed as a wound healing that has combined of effect of both component and easy to use. The purpose of this study was to determine the physical stability and irritability of chitosan–A. vera spray gel. Methods The spray gel stability test wa

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Ermawati, Nur. "UJI IRITASI SEDIAAN GEL ANTIJERAWAT FRAKSI LARUT ETIL ASETAT EKSTRAK ETANOL DAUN BINAHONG (Anredera cordiofolia (Ten.) Steenis) PADA KELINCI." Pena Jurnal Ilmu Pengetahuan dan Teknologi 32, no. 2 (2018): 33. http://dx.doi.org/10.31941/jurnalpena.v32i2.804.

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<pre><em>Gel is a pharmaceutical preparation with good penetration power and long contact time with skin so it can overcome the problem of acne</em><em>. </em><em>Gelling agent is the main ingredient in gel preparation. </em><em>Skin irritation test </em><em>is an important part of the pharmaceutical preparation procedures</em><em>. </em><em>In Material Safety Data Sheet states that Hydroxy Propyl Methyl Cellulose (HPMC) and Carbopol can cause skin irritation problems.</em><em> </em><em>

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Bagchi, Debasis, Yasuhiro Shikishima, Orie Yoshinari, et al. "Safety and Toxicological Evaluation of a Novel Citrus Sudachi Extract Powder." Functional Foods in Health and Disease 6, no. 10 (2016): 677. http://dx.doi.org/10.31989/ffhd.v6i10.301.

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Background: Citrus sudachi, an evergreen tree found primarily in the prefecture of Tokushima, Japan, is a widely used popular citrus fruit used in cooking and also consumed as a juice. Citrus sudachi peels are rich in flavonoids including sudachitin (5,7,4’-trihydroxy-6,8,3’-trimethoxyflavone), and exhibit potent antioxidant, antimicrobial and anti-diabetic properties, while several limonoids and their glucosides are found in its seeds. We examined the broad spectrum safety of a novel light yellow to golden yellow Citrus sudachi Extract Powder (organic, nutritive) from the dried fruit rind (25

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Arifin, Arfiani, Nur Ida, and Rosmiyanti Rosmiyanti. "FORMULASI DAN UJI IRITASI SEDIAAN LULUR KRIM CANGKANG SOTONG (Sepia sp.) TERHADAP KELINCI (Oryctolagus cuniculus)." Jurnal Riset Kefarmasian Indonesia 5, no. 1 (2023): 68–83. http://dx.doi.org/10.33759/jrki.v5i1.359.

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Cuttlefish shell (Sepia sp.) contains calcium carbonate which has benefits in removing dead skin cells, regulating skin pigmentation, shrinking pores, removing acne and black spots and making skin brighter. The aims of this study were to formalize dosage form of cuttlefish shell cream scrub (Sepia sp.) that meets the physical quality requirements and to determine the potential irritation of cuttlefish shell (Sepia sp.) cream scrubs in rabbits (Oryctolagus cuniculus). Reseacrh methods include the dosage form of cuttlefish shell powder using sieve mesh 44, cream scrub formulations with variation

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Jírová, Dagmar, David Basketter, Manfred Liebsch, et al. "Comparison of human skin irritation patch test data within vitroskin irritation assays and animal data." Contact Dermatitis 62, no. 2 (2010): 109–16. http://dx.doi.org/10.1111/j.1600-0536.2009.01640.x.

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Hyun, Su Bin, Sungmin Bae, and Chang-Gu Hyun. "Antioxidant Activities of Jeju Wax Apple (Syzygium samarangense) and Safety of Human Keratinocytes and Primary Skin Irritation Test." Cosmetics 7, no. 2 (2020): 39. http://dx.doi.org/10.3390/cosmetics7020039.

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This study analyzed the antioxidant activity, cell viability, and human skin primary irritation test using the hot-water extracts of the Syzygium samarangense. As a result of the recent warmer climate, tropical plants have flourished on Jeju Island, and S. samarangense is one of these plants known to have biological activities. In this study, the hot-water extract of S. samarangense leaf and branch was analyzed. Antioxidant activity was measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethyl-benzthiazoline-6-sulfonic acid)) assays, and the DMPD (dimethyl-4-phenylenedi

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Lu, Huai Xiu, Bin Deng, Long Quan Shao, et al. "A New Type of Colored Alumina/Glass Composite Biological Safety Assessment - Oral Mucous Membrane Irritation and Skin Sensitivity Tests." Advanced Materials Research 177 (December 2010): 462–65. http://dx.doi.org/10.4028/www.scientific.net/amr.177.462.

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Objective: to describe and demonstrate an oral mucosa irritation and a skin sensitivity test for evaluation of the biological safety of a colored alumina/glass composite (AGC). Methods: colored alumina/glass composite and control materials were sutured to mucine bilateral cheek pouch mucosa, in a position for gross observation and for histological and cytological examination; skin irritation activity of the composite was tested by intradermal injection of a guinea pig test solution, by inducing, enhancing, and stimulating the allergic response observed after local tissue exposure. Results: col

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Ikeda, Hidefumi, and Hideki Nishiura. "Study of in vitro Skin Irritation Test Targeted for Sensitive Skin." Journal of Society of Cosmetic Chemists of Japan 47, no. 1 (2013): 9–18. http://dx.doi.org/10.5107/sccj.47.9.

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Ryu, Y. H., K. M. Jung, S. H. Lee, K. M. Lim, and B. H. Kim. "Optimized in vitro skin irritation test using reconstructed human skin equivalent model, Keraskin(." Toxicology Letters 196 (July 2010): S140. http://dx.doi.org/10.1016/j.toxlet.2010.03.487.

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Fentem, Julia H., and Philip A. Botham. "ECVAM's Activities in Validating Alternative Tests for Skin Corrosion and Irritation." Alternatives to Laboratory Animals 30, no. 2_suppl (2002): 61–67. http://dx.doi.org/10.1177/026119290203002s09.

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ECVAM has funded and managed validation studies on in vitro tests for skin corrosion, resulting in the validities of four in vitro tests being endorsed by the ECVAM Scientific Advisory Committee: the rat skin transcutaneous electrical resistance (TER) assay, two tests based on the use of commercial reconstituted human skin equivalents, EPISKIN™ and EpiDerm™, and another commercially-produced test, CORROSITEX®. In the European Union (EU), a new test method on skin corrosion (B.40), incorporating the rat skin TER and human skin model assays, was included in Annex V of Directive 67/548/EEC in mid

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Farage, Miranda, and Angela Stadler. "Cumulative irritation patch test of sanitary pads on sensitive skin." Journal of Cosmetic Dermatology 4, no. 3 (2005): 179–83. http://dx.doi.org/10.1111/j.1473-2165.2005.00307.x.

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Surjanto, Surjanto, Ridwanti Batubara, and Dedy Syahputera Rangkuti. "Safety Test of Agarwood Leaves Tea (Aquilaria malaccencis Lamk.) Through Skin Sensitization Test on Albino Rabbit." Open Access Macedonian Journal of Medical Sciences 7, no. 22 (2019): 3896–99. http://dx.doi.org/10.3889/oamjms.2019.528.

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BACKGROUND: Agarwood tree (Aquilaria malaccensis Lamk) is a tree from the Thymeleaceae tribe that has a high selling value. The part that is often used is the sapwood of the agarwood tree, used as a base for perfumes and traditional medicines. Agarwood farmers in Langkat have used their leaves as tea drinks, brewing aloes leaves. Before being widely produced by the community, it is necessary to test the safety of agarwood leaf tea products through skin sensitization test. AIM: To find out whether steeping aloes leaves irritate the skin or not. METHODS: The test method using test an

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Kim, Sang Ho, Myeong kyu Park, Ja Kyung Seol, et al. "Evaluation of potential eye or skin irritation/corrosion in rabbit exposed to TiO2 photocatalyst (GST)." Environmental Analysis Health and Toxicology 36, no. 3 (2021): e2021022. http://dx.doi.org/10.5620/eaht.2021022.

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TiO2 NPs photocatalyst is widely used in a variety of applications and products in the environmental and energy fields, including self-cleaning surfaces, air and water purification systems, sterilization, hydrogen evolution, and photoelectrochemical conversion. The possible biological and safety effects of TiO2 dermal exposure and absorption have not been well studied and more investigations on the potential health hazards of the TiO2 are needed. This study aimed to investigate potential effect of local lesions (eye and skin irritation/corrosion) for new TiO2 material powder, GST produced thro

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Lee, Ye-Jin, Yang Xu, and Chang-Gu Hyun. "5,7-Dihydroxy-4-Methylcoumarin as a Functional Compound for Skin Pigmentation and Human Skin Safety." Pharmaceuticals 18, no. 4 (2025): 463. https://doi.org/10.3390/ph18040463.

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Background/Objectives: This study aims to investigate the effects of 5,7-dihydroxy-4-methylcoumarin (5,7D-4MC) on melanogenesis in B16F10 murine melanoma cells and to evaluate its safety as a potential ingredient for functional cosmetics and therapeutic agents targeting pigmentation-related disorders. Method: The cytotoxicity of 5,7D-4MC was assessed using an MTT assay, and melanin content and tyrosinase activity were measured at different concentrations (25, 50, 100 µM). Western blot analyses were conducted to evaluate the expression of key melanogenesis-related proteins (TYR, TRP-1, TRP-2, a

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Ardhany, Syahrida Dian, Susi Novaryatiin, Mohammad Rizki Fadhil Pratama, and Zulkhurnain Utar. "IRRITATION TEST OF BAWANG DAYAK (Eleutherine bulbosa(Mill.) Urb.) EXTRACT CREAM WITH HUMAN PATCH TEST METHOD." Jurnal Farmasi Sains dan Praktis 7, no. 1 (2021): 74–80. http://dx.doi.org/10.31603/pharmacy.v7i1.4854.

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Topical agents indicated for the treatment of acne have the potential to cause irritation or allergic contact dermatitis. This study investigates the irritancy potential of anti-acne cream of bawang dayak (Eleutherine bulbosa (Mill.) Urb.) previously tested for microbiological effectiveness with the lowest concentration of 5% and the highest concentration of 20%. The method used in this study is the human patch test. A total of 20 volunteers were recruited for the patch test study, testing the cream. The result showed that all volunteers did not experience irritation both in the 5% or 20% bawa

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Prihantini, Malinda. "Molecular Complex Gel Formulation of Glycolic Acid-Chitosan Combination with Variations Of Xanthan Gum Base Concentration And Irritation Test." Jurnal Ilmu Farmasi dan Farmasi Klinik 21, no. 2 (2024): 285–93. https://doi.org/10.31942/jiffk.v21i2.12331.

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External causes such as UV exposure cause more severe skin damage than internal factors, hence it is essential to utilize cosmeceutical treatments containing AHAs such as glycolic acid. Because using glycolic acid without modification or monitoring might cause skin irritation, a molecular compound of glycolic acid-chitosan is created in a gel preparation with a xanthan gum foundation to improve consumer comfort and effectiveness. The goal of this study was to create a molecular complex gel of glycolic acid and chitosan (AG-CS) with varying xanthan gum concentrations and conduct irritation test

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Susanti, Ni Made Pitri, I. Nyoman Triadhi Wisesa, and Chenme Juwianti. "IN SILICO TOXICITY OF ANTHOCYANIN COMPOUNDS (CYANIDIN AND PEONIDIN)." Journal of Pharmaceutical Science and Application 5, no. 1 (2023): 53. http://dx.doi.org/10.24843/jpsa.2023.v05.i01.p07.

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Backgrounds: Cyanidin and peonidin, anthocyanin compounds, have many in silico, in vitro, and in vivo activities, including antioxidant, anticancer, antihyperlipidemic, and antidiabetic. Toxicity testing is carried out to determine the potential hazard that may be produced by the test compound. Objective: This study was aimed to determine the in silico toxicity of anthocyanins (cyanidin and peonidin) using Toxtree v3.1.0 software. Methods: In silico toxicity testing was carried out using 2D structures of cyanidin and peonidin with Cramer rules, Verhaar scheme, Benigni/Bossa rulebase, Kroes TTC

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Rajesh, Kumawat, Sachdev Mukta, Ramnane Mukesh, B. Swathi, and U. V. Babu. "Assessment of Cumulative Irritation and Sensitization Potential of Himalaya's Baby Skin Care Products in Adult Healthy Volunteers by Human Repeat Insult Patch Test (HRIPT)." International Journal of Toxicological and Pharmacological Research 12, no. 6 (2022): 174–84. https://doi.org/10.5281/zenodo.11669534.

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This study was to assess the mildness, gentleness and allergenic potency of 18 marketed baby skin care products. This open-label, controlled, single-center, HRIPT was conducted in 2 studies as per BIS 4011:2018 guidelines. Each study included 200 healthy participants in the age of 18-65years (of either sex). 10 products in study 1 and 8 products in study 2 were evaluated in different phases. Induction phase, products were repeatedly applied on the back of study subjects in the form of occlusive patch for 24 hours for 3 times a week, for 3 weeks. The reactions were assessed 48 hours after every

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Wilhelm, K. P., F. Pasche, C. Surber, and H. I. Maibach. "Sodium hydroxide-induced subclinical irritation. A test for evaluating stratum corneum barrier function." Acta Dermato-Venereologica 70, no. 6 (1990): 463–67. http://dx.doi.org/10.2340/00015555-70463467.

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This report concerns the development of a short, simple, non-invasive test for assessing sensitivity to irritant dermatitis. Application of NaOH (0.005-2.0 mol/l) to human skin resulted in significantly greater skin surface water loss directly after exposure (1-15 min) than of control (water). The increase in skin surface water loss after NaOH application was dose-dependent (0.005-0.1 mol/l) and application time-dependent (1-10 min). Application times exceeding 10 min did not further increase skin surface water loss and doses higher than 0.1 mol/l reversed the effect on skin surface water loss

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Walters, Russel M., Lisa Gandolfi, M. Catherine Mack, et al. "In Vitro Assessment of Skin Irritation Potential of Surfactant-based Formulations by Using a 3-D Skin Reconstructed Tissue Model and Cytokine Response." Alternatives to Laboratory Animals 44, no. 6 (2016): 523–32. http://dx.doi.org/10.1177/026119291604400611.

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The personal care industry is focused on developing safe, more efficacious, and increasingly milder products, that are routinely undergoing preclinical and clinical testing before becoming available for consumer use on skin. In vitro systems based on skin reconstructed equivalents are now established for the preclinical assessment of product irritation potential and as alternative testing methods to the classic Draize rabbit skin irritation test. We have used the 3-D EpiDerm™ model system to evaluate tissue viability and primary cytokine interleukin-1α release as a way to evaluate the potentia

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Jirova, Dagmar, David Basketter, Hana Bendová, et al. "Comparison of results of the 4h human skin irritation patch test and in vitro human skin irritation test (EpiDerm SIT) with existing classifications of chemicals." Toxicology Letters 180 (October 2008): S108. http://dx.doi.org/10.1016/j.toxlet.2008.06.434.

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Fusco, Laura, Marina Garrido, Cristina Martín, et al. "Skin irritation potential of graphene-based materials using a non-animal test." Nanoscale 12, no. 2 (2020): 610–22. http://dx.doi.org/10.1039/c9nr06815e.

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