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1

Haenni, Marisa, Laure Galofaro, Mathilde Ythier, et al. "Penicillin-Binding Protein Gene Alterations in Streptococcus uberis Isolates Presenting Decreased Susceptibility to Penicillin." Antimicrobial Agents and Chemotherapy 54, no. 3 (2010): 1140–45. http://dx.doi.org/10.1128/aac.00915-09.

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ABSTRACT Streptococcus uberis is an environmental pathogen commonly causing bovine mastitis, an infection that is generally treated with penicillin G. No field case of true penicillin-resistant S. uberis (MIC > 16 mg/liter) has been described yet, but isolates presenting decreased susceptibility (MIC of 0.25 to 0.5 mg/liter) to this drug are regularly reported to our laboratory. In this study, we demonstrated that S. uberis can readily develop penicillin resistance in laboratory-evolved mutants. The molecular mechanism of resistance (acquisition of mutations in penicillin-binding protein 1A
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2

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, et al. "Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen." Journal of Microbial & Biochemical Technology 7, no. 5 (2015): 269–73. https://doi.org/10.4172/1948-5948.1000223.

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Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr. B strain was used in this experiment bearing the American Type Culture Collection (ATCC 12386) number and stored according to the recommended storage protocol. The revived and lyophilized state of ATCC strains of S. agalactiae gr. B were selected for the stud
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3

Trivedi, Mahendra Kumar, Alice Branton, Dahryn Trivedi, et al. "Effect of Biofield Energy Treatment on Streptococcus group B: A Postpartum Pathogen." Journal of Microbial & Biochemical Technology 7, no. 5 (2015): 269–73. https://doi.org/10.5281/zenodo.167155.

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Streptococcus agalactiae group B (S. agalactiae gr. B) is widespread in nature mainly causes bacterial septicemia and neonatal meningitis. The current study was attempted to investigate the effect of biofield treatment on S. agalactiae gr. B with respect of antimicrobial sensitivity, biochemical reactions and bio typing. S. agalactiae gr. B strain was used in this experiment bearing the American Type Culture Collection (ATCC 12386) number and stored according to the recommended storage protocol. The revived and lyophilized state of ATCC strains of S. agalactiae gr. B were selected for the stud
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4

Oliveira, Pâmella Schramm, Aline Rossato, Larissa da Silva Silveira, et al. "GRAPHENE OXIDE AND REDUCED GRAPHENE OXIDE." International Journal for Innovation Education and Research 9, no. 12 (2021): 142–69. http://dx.doi.org/10.31686/ijier.vol9.iss12.3572.

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To present a possible new alternative for wound treatment, this work evaluated the biological safety and therapeutic efficacy of graphene oxide (GO) and reduced graphene oxide (rGO) nanoparticles (NPs). First, the nanostructures were studied in silico and showed to be able to inhibit the production of some pro-inflammatory cytokines and stimulate the production of the anti-inflammatory cytokine IL-10, especially rGO. The results of the morphological and structural characterization of GO NPs synthesized from the Hummers method and reduced by ascorbic acid, were consistent with the literature, c
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5

Faccone, Diego, Florencia Lalonardi, Sofia Abel, et al. "Multiple-Clones of Streptococcus agalactiae harbouring lnuB gene." Journal of Infection in Developing Countries 4, no. 09 (2010): 580–82. http://dx.doi.org/10.3855/jidc.941.

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Six S. agalactiae displaying an L-phenotype were recovered from recto-vaginal screening culture from 3 hospitals. S. agalactiae isolates showed susceptible MICs to erythromycin, MIC≤0.12mg/L, and azithromycin, MIC≤0.25mg/L, but resistant MICs to clindamycin, MIC=4mg/L, and lincomycin, MIC=64-128mg/L. Isolates were PCR positive for lnuB gene. Four clones were discriminated by ApaI-PFGE (n): A (3), B (1), C (1) and D (1). Here we describe for the first time the polyclonal emergence of S. agalactiae harboring the lnuB gene in Latin-America.
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6

Han, Long, Wen Zheng, Zhangjiang He, Shengyan Qian, Xiaoya Ma, and Jichuan Kang. "Endophytic fungus Biscogniauxia petrensis produces antibacterial substances." PeerJ 11 (June 7, 2023): e15461. http://dx.doi.org/10.7717/peerj.15461.

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Widespread drug resistance and limited antibiotics challenge the treatment of pathogenic bacteria, which leads to a focus on searching for new antimicrobial lead compounds. We found the endophytic fungus Biscogniauxia petrensis MFLUCC14-0151 from the medicinal plant Dendrobium harveyanum had antibacterial activity for the first time. This work aimed to reveal the capacity of Biscogniauxia petrensis MFLUCC14-0151 against foodborne pathogenic bacteria and identify its bioactive substances. Bioassay-guided isolation led to the discovery of six infrequent active monomers, including (10R)-Xylariter
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7

Syawal, Henni, Rahman Karnila, Angraika Dirta, and Ronal Kurniawan. "Ekstrak Daun Rhizophora sp. Menghambat Pertumbuhan Bakteri Streptococcus agalactiae dan Edwarsiella tarda (RHIZOPHORA SP. LEAF EXTRACT INHIBITS THE GROWTH OF Streptococcus agalactiae AND Edwarsiella tarda)." Jurnal Veteriner 18, no. 4 (2018): 604. http://dx.doi.org/10.19087/jveteriner.2017.18.4.604.

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This study aimed to observe the inhibition capability of Rhizophora sp. leaf extract towards the Streptococcus agalactiae and Edwardsiella tarda bacteria. Rhizophora sp. leaf was extracted using ethanol. Inhibition action of Rhizophora sp. leaf extract towards Streptococcus agalactiae and Edwardsiella tarda was tested on TSA solid media. The concentration of Rhizophora sp. leaf extract used were 1000, 2000, 3000, 4000, 5000, 6000, 7000, 8000, 9000, 10000 ppm, and novobiocin antibiotic was used as a control. Each treatment was conducted three times. MIC (minimum inhibition concentration) was pe
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8

Šukele, Renāte, Ance Bārzdiņa, Rudīte Koka, et al. "Antibacterial Activity of Tanacetum vulgare L. Extracts against Clinical Isolates of Bovine Mastitis." Applied Sciences 13, no. 5 (2023): 3369. http://dx.doi.org/10.3390/app13053369.

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A bovine mastitis is an infectious disease, which is usually treated with antibiotics. Alternatively, herbal medicine has been proposed due to bacterial resistance. The aim of this study was to determine the antibacterial activity of the acetonic and ethanolic extracts of dried flowers and leaves of Tanacetum vulgare L. against bovine mastitis-inducing clinical isolates such as Escherichia coli, Streptococcus agalactiae, Streptococcus uberis, Serratia liquefaciens, Staphylococcus aureus, and reference cultures of S. aureus and E. coli. The extracts of T. vulgare showed partial antibacterial ac
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9

Betriu, C., E. Culebras, I. Rodríguez-Avial, M. Gómez, B. A. Sánchez, and J. J. Picazo. "In Vitro Activities of Tigecycline against Erythromycin-Resistant Streptococcus pyogenes and Streptococcus agalactiae: Mechanisms of Macrolide and Tetracycline Resistance." Antimicrobial Agents and Chemotherapy 48, no. 1 (2004): 323–25. http://dx.doi.org/10.1128/aac.48.1.323-325.2004.

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ABSTRACT The activity of tigecycline was tested against erythromycin-resistant streptococci (107 Streptococcus pyogenes and 98 Streptococcus agalactiae strains). The presence of erythromycin and tetracycline resistance genes was determined by PCR. Among S. pyogenes strains the most prevalent gene was mef(A) (91.6%). The erm(B) gene was the most prevalent (65.3%) among S. agalactiae strains. Tigecycline proved to be very active against all the isolates tested (MIC at which 90% of the isolates tested were inhibited, 0.06 μg/ml), including those resistant to tetracycline.
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10

Achard, Adeline, Corinne Villers, Vianney Pichereau, and Roland Leclercq. "New lnu(C) Gene Conferring Resistance to Lincomycin by Nucleotidylation in Streptococcus agalactiae UCN36." Antimicrobial Agents and Chemotherapy 49, no. 7 (2005): 2716–19. http://dx.doi.org/10.1128/aac.49.7.2716-2719.2005.

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ABSTRACT Streptococcus agalactiae UCN36 was resistant to lincomycin (MIC = 16 μg/ml) but susceptible to clindamycin (MIC = 0.12 μg/ml) and erythromycin (MIC = 0.06 μg/ml). A 4-kb HindIII fragment was cloned from S. agalactiae UCN36 total DNA on plasmid pUC18 and introduced into Escherichia coli AG100A, where it conferred resistance to lincomycin. The sequence analysis of the fragment showed the presence of a 1,724-bp element delineated by imperfect inverted repeats (22 of 25 bp) and inserted in the operon for capsular synthesis of S. agalactiae UCN36. This element carried two open reading fram
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11

Yang, Qingwen, Chenghuan Zhang, Xuesong Liu, et al. "The pharmacokinetics and pharmacodynamics of cefquinome against Streptococcus agalactiae in a murine mastitis model." PLOS ONE 18, no. 1 (2023): e0278306. http://dx.doi.org/10.1371/journal.pone.0278306.

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Cefquinome is a new generation cephalosporin that is effective in the treatment of mastitis in animals. In this study, we evaluated the associations between the specific pharmacokinetics and pharmacodynamics (PK/PD) of cefquinome and its antibacterial activity against Streptococcus agalactiae in a mouse model of mastitis. After a single intramammary dose of cefquinome (30, 60, 120, and 240 μg/mammary gland), the concentration of cefquinome in plasma was analysed by liquid chromatography with tandem mass spectrometry (HPLC/MS–MS). The PK parameters were calculated using a one-compartment first-
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12

Li, Guiqiu, Ying Wei, Yan Guo, et al. "Omadacycline Efficacy against Streptococcus Agalactiae Isolated in China: Correlation between Resistance and Virulence Gene and Biofilm Formation." Computational Intelligence and Neuroscience 2022 (April 25, 2022): 1–8. http://dx.doi.org/10.1155/2022/7636983.

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This study aimed to evaluate the activity, resistance, clonality of MIC distribution, and the correlation between virulence and resistance genes and biofilm formation of omadacycline (OMC) in clinics for Streptococcus agalactiae isolates from China. 162 isolates were collected retrospectively in China. The S. agalactiae were collected from the body’s cervical secretions, wound secretions, ear swabs, secretions, semen, venous blood, cerebrospinal fluid, pee, etc. The MIC of OMC against S. agalactiae was determined by broth microdilution. The inhibition zone diameters of OMC and other common ant
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13

Shan, Yuxue, Na Yang, Da Teng, et al. "Recombinant of the Staphylococcal Bacteriophage Lysin CHAPk and Its Elimination against Streptococcus agalactiae Biofilms." Microorganisms 8, no. 2 (2020): 216. http://dx.doi.org/10.3390/microorganisms8020216.

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Bovine mastitis is the most important infectious disease, causing significant losses in the dairy industry, in which Streptococcus agalactiae is a major pathogen. In this study, lysin CHAPk, derived from bacteriophage K, was expressed heterogeneously, and its antimicrobial and anti-biofilm effects against S. agalactiae isolated from bovine mastitis were further analyzed. CHAPk was expressed in Escherichia coli BL21 (DE3), in which the purified yield of CHAPk was up to 14.6 mg/L with the purity of 95%. Time-killing kinetic curves showed that CHAPk fastly killed S. agalactiae in TSB medium and i
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14

Arain, Muhammad Bilawal, Ali Gul Soomro, Shamsuddin Bughio, Rehana Buriro, Aijaz Ali, and Saeed Ahmed Soomro. "Antibacterial Potential of Aloe vera against Staphylococcus aureus and Streptococcus agalactiae isolated from Mastitic Milk." Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences 59, no. 2 (2022): 71–78. http://dx.doi.org/10.53560/ppasb(59-2)686.

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The extensive use of antibiotics has developed antibacterial resistance and also may cause toxic effects (hepatotoxicity, nephrotoxicity) on vital organs. To overcome this problem, Scientists gain attention towards medicinal plants. Pure Aloe vera (AV) is a common alternative antimicrobial medicine, hence, the current study was conducted to explore its antibacterial potential and compared it with a commonly used antibiotic amoxicillin. During this study, clinically positive mastitis milk samples (n=50) were collected from buffaloes, after microbial culture analysis. Various concentrations (C1=
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15

Bray, Beverley A., Iain C. Sutcliffe, and Dean J. Harrington. "Impact of lgt mutation on lipoprotein biosynthesis and in vitro phenotypes of Streptococcus agalactiae." Microbiology 155, no. 5 (2009): 1451–58. http://dx.doi.org/10.1099/mic.0.025213-0.

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Although Streptococcus agalactiae, the group B Streptococcus, is a leading cause of invasive neonatal disease worldwide the molecular basis of its virulence is still poorly understood. To investigate the role of lipoproteins in the physiology and interaction of this pathogen with host cells, we generated a mutant S. agalactiae strain (A909ΔLgt) deficient in the Lgt enzyme and thus unable to lipidate lipoprotein precursors (pro-lipoproteins). The loss of pro-lipoprotein lipidation did not affect the viability of S. agalactiae or its growth in several different media, including cation-depleted m
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16

Fléchard, Maud, and Philippe Gilot. "Physiological impact of transposable elements encoding DDE transposases in the environmental adaptation of Streptococcus agalactiae." Microbiology 160, no. 7 (2014): 1298–315. http://dx.doi.org/10.1099/mic.0.077628-0.

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We have referenced and described Streptococcus agalactiae transposable elements encoding DDE transposases. These elements belonged to nine families of insertion sequences (ISs) and to a family of conjugative transposons (TnGBSs). An overview of the physiological impact of the insertion of all these elements is provided. DDE-transposable elements affect S. agalactiae in a number of aspects of its capability to adapt to various environments and modulate the expression of several virulence genes, the scpB–lmB genomic region and the genes involved in capsule expression and haemolysin transport bei
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17

Samen, Ulrike, Beate Heinz, Heike Boisvert, Bernhard J. Eikmanns, Dieter J. Reinscheid, and Frédéric Borges. "Rga is a regulator of adherence and pilus formation in Streptococcus agalactiae." Microbiology 157, no. 8 (2011): 2319–27. http://dx.doi.org/10.1099/mic.0.044933-0.

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Streptococcus agalactiae is the leading cause of bacterial sepsis and meningitis in neonates and is also the causative agent of several serious infections in immunocompromised adults. S. agalactiae encounters multiple niches during an infection, suggesting that regulatory mechanisms control the expression of specific virulence factors in this bacterium. The present study describes the functional characterization of a gene from S. agalactiae, designated rga, which encodes a protein with significant similarity to members of the RofA-like protein (RALP) family of transcriptional regulators. After
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Wu, Yankang, Na Yang, Ruoyu Mao, Ya Hao, Da Teng, and Jianhua Wang. "In Vitro Pharmacodynamics and Bactericidal Mechanism of Fungal Defensin-Derived Peptides NZX and P2 against Streptococcus agalactiae." Microorganisms 10, no. 5 (2022): 881. http://dx.doi.org/10.3390/microorganisms10050881.

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(1) Background: Based on the hazard of Streptococcus agalactiae to human and animal health and the increasing drug resistance, it is urgent to develop new antimicrobial agents with high bactericidal activity and low drug resistance against S. agalactiae. This study aims to investigate in vitro pharmacodynamics and bactericidal mechanism of fungal defensin-derived peptides NZX and P2 against S. agalactiae. (2) Methods: Minimum inhibitory concentration (MIC) and mutant prevention concentration (MPC) were determined by broth dilution method and AGAR plate dilution method. Cell membrane integrity
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Pritchard, David G., Shengli Dong, John R. Baker, and Jeffrey A. Engler. "The bifunctional peptidoglycan lysin of Streptococcus agalactiae bacteriophage B30." Microbiology 150, no. 7 (2004): 2079–87. http://dx.doi.org/10.1099/mic.0.27063-0.

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A group B streptococcal (GBS) bacteriophage lysin gene was cloned and expressed in Escherichia coli. The purified recombinant enzyme, calculated to have a molecular mass of 49 677 Da, lysed GBS cells. The susceptibility of GBS cells to lysis by the enzyme depended upon the growth stage at which they were harvested, with early exponential phase cells most sensitive. Calcium ions enhanced the activity of the enzyme. The enzyme also lysed other β-haemolytic streptococci, including groups A, C, E and G streptococci, but not common oral streptococci, including Streptococcus mutans. The generation o
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Dong, Jing, Yuze Zhang, Qiuhong Yang, Yongtao Liu, Shun Zhou, and Xiaohui Ai. "Fraxetin Targeting to Sortase A Decreases the Pathogenicity of Streptococcus agalactiae to Nile Tilapia." Animals 14, no. 9 (2024): 1337. http://dx.doi.org/10.3390/ani14091337.

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Sortase A (SrtA) is responsible for anchoring surface proteins to the cell wall, and has been identified as a promising target developing anti-infective drugs of Gram-positive bacteria. The aim of the study was to identify inhibitors of Streptococcus agalactiae (S. agalactiae) SrtA from natural compounds to overcome the spread of antibiotic resistance in aquaculture. Here, we found that the MIC of fraxetin against S. agalactiae was higher than 256 μg/mL, indicating that fraxetin had no anti- S. agalactiae activity. But fraxetin could dose-dependently decrease the activity of SrtA in vitro at c
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Lorca, Guillermo, Diego Ballestero, Elisa Langa, and María Rosa Pino-Otín. "Enhancing Antibiotic Efficacy with Natural Compounds: Synergistic Activity of Tannic Acid and Nerol with Commercial Antibiotics against Pathogenic Bacteria." Plants 13, no. 19 (2024): 2717. http://dx.doi.org/10.3390/plants13192717.

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The search for synergies between natural products and commercial antibiotics is a promising strategy against bacterial resistance. This study determined the antimicrobial capacity of Nerol (NE) and Tannic Acid (TA) against 14 pathogenic bacteria, including ESKAPE pathogens. TA exhibited the lowest Minimum Inhibitory Concentrations (MICs) at 162.5 µg/mL against Pasteurella aerogenes and 187.5 µg/mL against Acinetobacter baumannii (WHO priority 1). NE showed its lowest MIC of 500 µg/mL against both Pasteurella aerogenes and Salmonella enterica. A total of 35 combinations of NE and 13 of TA with
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Paulová, Tereza, Lucie Malíková, Davide Lanzoni, et al. "Inhibitory Potential of Cannabis Biomass Extracts on Livestock-Associated Staphylococcal and Streptococcal Pathogens." Microorganisms 13, no. 2 (2025): 432. https://doi.org/10.3390/microorganisms13020432.

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Diseases caused by staphylococci and streptococci are a serious burden on livestock production, causing significant losses. In addition, the associated antibiotic resistance of these pathogens often makes treatment impossible or prolonged. Cannabis sativa L. contains many compounds with antibacterial properties and shows great potential as a natural antimicrobial agent for agricultural use against both of these bacterial species. The aim of this study was to compare the in vitro antibacterial activity of ethanol extracts from five cultivars of hemp, namely, Bialobrzeskie, Felina 32, Futura 75,
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Patel, Krina M., Bhavdip B. Parmar, Kamlesh A. Sadariya, and Shailesh K. Bhavsar. "Assessment of in vitro antibacterial activity and MIC of cinnamon bark powder ethanolic and aqueous extracts against bacteria." Journal of Phytopharmacology 11, no. 5 (2022): 324–29. http://dx.doi.org/10.31254/phyto.2022.11502.

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The study was planned to evaluate the in vitro antibacterial activity and minimum inhibitory concentration (MIC) of cinnamon (Cinnamomum zeylanicum) bark powder ethanolic and aqueous extracts. Screening of cinnamon powder ethanolic and aqueous extracts for antibacterial sensitivity and MIC against Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus, Listeria monocytogenes, Escherichia coli and Pseudomonas aeruginosa was carried out. ABST was performed by the disc diffusion method. The cinnamon powder ethanolic and aqueous extracts were suspended in a solution containing 10% dimeth
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Nguyễn Thị Trúc Quyên, Lê Linh Chi, Đoàn Văn Cường та ін. "Khả năng đối kháng vi khuẩn <i>Streptococcus agalactiae</i> phân lập trên cá rô phi (<i>Oreochromis</i> spp.) bởi một số cao chiết thảo dược". Tạp chí Khoa học - Công nghệ Thủy Sản, Trường Đại học Nha Trang, № 03 (30 вересня 2019): 124–32. http://dx.doi.org/10.53818/jfst.03.2019.375.

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Nghiên cứu đánh giá khả năng ức chế tăng trưởng của vi khuẩn Streptococcus agalactiae phân lập được trên cá rô phi của một số cao chiết có nguồn gốc thảo dược. Dịch chiết của năm loại thảo dược (quế, gừng, xuyên tâm liên, diếp cá, tía tô) được pha trong dung môi ethanol 96% và methanol 99,8%, sau khi xử lý nhiệt, lọc và cô quay chân không tạo được các cao chiết có nồng độ 2000 mg/ml. Kết quả cho thấy, cao chiết vỏ quế (trong ethanol 96% hoặc methanol 99,8%) cho hiệu quả kháng khuẩn cao nhất với cả Streptococcus agalactiae SA3 và SA4, ở mức đối kháng mạnh với đường kính vòng vô khuẩn lần lượt l
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Sela, Floresha, Marija Karapandzova, Gjose Stefkov, et al. "Chemical composition and antimicrobial activity of berry essential oil of Juniperus oxycedrus L. (Cupressaceae) grown wild in Republic of Macedonia." Macedonian Pharmaceutical Bulletin 59 (April 2013): 41–48. http://dx.doi.org/10.33320/maced.pharm.bull.2013.59.005.

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Chemical composition and antimicrobial activity of essential oil isolated from berries from 2 different samples of Juniperus oxycedrus L. (Cupressaceae), growing wild in Republic of Macedonia was investigated. Performing GC/FID/MS analysis, one hundred components were identified, representing 96.0-98.95% of the oil. The major components were α-pinene (22.54- 27.12%), myrcene (11.26- 15.13%) and limonene (2.78-18.06%). Antimicrobial screening of the J. oxycedrus essential oils was made by disc diffusion and broth dilution method against 16 bacterial isolates of Gram positive and Gram negative b
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Yazdani, Morteza, Annamária Kincses, and Judit Hohmann. "Lanostane Triterpenes with Antimicrobial Activity: A Study of the Pholiol Series from the Hungarian Edible Mushroom Pholiota populnea." Acta Pharmaceutica Hungarica 94, no. 1 (2024): 1–6. https://doi.org/10.33892/aph.2024.94.1-6.

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Aims: Pholiota populnea is an edible macrofungus, distributed worldwide wherever cottonwood occurs. This study aimed to explore the potential antimicrobial properties of isolated lanostane triterpenes from P. populnea, namely pholiols A-D, E, G, H, J, L, and Q. Methods: A diverse range of microorganisms, including Gram-negative, and Gram-positive bacterial and fungal strains were employed to assess the antimicrobial activity of these natural triterpenes. Results: Among the investigated compounds, pholiol C [3β-acetoxy-2α-(3-hydroxy-3-methylglutaroyloxy)-12β,25-dihydroxylanosta-8-en-24-one] exh
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Petinaki, Efthymia, Véronique Guérin-Faublée, Vianney Pichereau, et al. "Lincomycin Resistance Gene lnu(D) in Streptococcus uberis." Antimicrobial Agents and Chemotherapy 52, no. 2 (2007): 626–30. http://dx.doi.org/10.1128/aac.01126-07.

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ABSTRACT Streptococcus uberis UCN 42, isolated from a case of bovine mastitis, was intermediately resistant to lincomycin (MIC = 2 μg/ml) while remaining susceptible to clindamycin (MIC = 0.06 μg/ml) and erythromycin. A 1.1-kb SacI fragment was cloned from S. uberis UCN 42 total DNA on plasmid pUC 18 and introduced into Escherichia coli AG100A, where it conferred resistance to both clindamycin and lincomycin. The sequence analysis of the fragment showed the presence of a new gene, named lnu(D), that encoded a 164-amino-acid protein with 53% identity with Lnu(C) previously reported to occur in
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Pangprasit, Noppason, Anyaphat Srithanasuwan, Witaya Suriyasathaporn, and Wasana Chaisri. "Antibacterial properties of lauric acid in combination with organic acids against major pathogens causing dairy mastitis." Veterinary Integrative Sciences 19, no. 1 (2020): 37–44. http://dx.doi.org/10.12982/vis.2021.003.

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The objective of this study was to determine the antibacterial properties of lauric acid in combination with acetic acid and lactic acid against major dairy mastitis pathogens including Staphylococcus aureus, Streptococcus agalactiae, Streptococcus uberis, Escherichia coli and Klebsiella spp. The antibacterial effect of each acid and the acid mixtures was evaluated by their minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) using broth microdilution method. The differences in, MIC and MBC values of lauric acid and acid mixtures for each pathogen were calculate
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Abdul Razak, Laith A., Nadirah Musa, Aya Jabar, and Najiah Musa. "Therapeutic potentials of Excoecaria agallocha against gram-positive and gram-negative fish bacterial pathogens." Journal of Ideas in Health 2, no. 2 (2019): 87–94. http://dx.doi.org/10.47108/jidhealth.vol2.iss2.21.

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Background: The present investigation of a mangrove plant, Excoecaria agallocha, which is a popular medicinal substitute for the treatment of microbial ailments, were evaluated for potential antimicrobial activity against pathogenic bacteria Escherichia coli and Streptococcus agalactiae in tilapia, Oreochromis niloticus.&#x0D; Methods: Antibacterial activity was performed using agar diffusion method, disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiotic susceptibility assays. Experimental fish fed diet containing 0 (control), 5,
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Traczewski, Maria M., and Steven D. Brown. "Proposed MIC and Disk Diffusion Microbiological Cutoffs and Spectrum of Activity of Retapamulin, a Novel Topical Antimicrobial Agent." Antimicrobial Agents and Chemotherapy 52, no. 11 (2008): 3863–67. http://dx.doi.org/10.1128/aac.00399-08.

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ABSTRACT Retapamulin, the first pleuromutilin antimicrobial agent approved for the topical treatment of skin infections in humans, was tested against 987 clinical isolates representing 30 species and/or resistance groups. MICs were determined along with disk diffusion zone diameters using a 2-μg disk. Population distribution and MIC versus disk zone diameter scattergrams were analyzed to determine microbiological MIC cutoff values and inhibition zone correlates. Minimum bactericidal concentrations were performed on a smaller subset of key species. The retapamulin MIC90 against 234 Staphylococc
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Pinto, Gabriele Marques, Juliana Barbosa Succar, Cristiane Pimentel Victorio, and Maria Cristina de Assis. "Plant volatiles from the Brazilian restinga with bactericidal activity against multiresistant bacteria." Concilium 22, no. 7 (2022): 598–612. http://dx.doi.org/10.53660/clm-724-769.

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This study aimed to evaluate the bactericidal activity of Myrtaceae foliar volatile compounds against strains of Pseudomonas aeruginosa (PAO-1) and Burkholderia cenocepacia (ET-12). Although these bacteria are clinically important to human health, they, along with other bacteria (Escherichia coli, Staphylococcus aureus and Streptococcus agalactiae), also have a high profile of antimicrobial resistance associated with bovine mastitis. The chemical composition of volatiles found in Myrtaceae foliar volatiles was also characterized. To accomplish this, the essential oil was extracted from dry lea
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Pinto, Gabriele Marques, Succar Juliana Barbosa, Cristiane Pimentel Victório, and Maria Cristina Assis. "Plant volatiles from the Brazilian restinga with bactericidal activity against multiresistant bacteria." Concilium 22, no. 7 (2022): 598–612. https://doi.org/10.5281/zenodo.15564479.

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This study aimed to evaluate the bactericidal activity of Myrtaceae foliar volatile compounds against strainsof Pseudomonas aeruginosa (PAO-1) and Burkholderia cenocepacia (ET-12). Although these bacteria areclinically important to human health, they, along with other bacteria (Escherichia coli, Staphylococcusaureus and Streptococcus agalactiae), also have a high profile of antimicrobial resistance associated withbovine mastitis. The chemical composition of volatiles found in Myrtaceae foliar volatiles was alsocharacterized. To accomplish this, the essential oil was extracted from dry leaves o
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Portal, Edward A. R., Caitlin Farley, Teresa Iannetelli, et al. "Agar-Dilution Is Comparable to Broth Dilution for MIC Determination in Streptococcus agalactiae." Antibiotics 14, no. 2 (2025): 156. https://doi.org/10.3390/antibiotics14020156.

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Background: Streptococcus agalactiae (Group B Streptococcus, GBS) is a leading cause of neonatal sepsis in high-income countries. While intrapartum antibiotic screening reduces this risk, increasing resistance to macrolides and lincosamides in Europe since the 1990s has limited therapeutic options for penicillin-allergic patients. Reports of reduced beta-lactam susceptibility in GBS further emphasise the need for robust antimicrobial resistance (AMR) surveillance. However, broth microdilution (BMD) methods are unsuitable for large-scale antimicrobial susceptibility testing (AST). Objective: To
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Châu Thuỳ, Phương, My Hồ Thị Kim, Nga Nguyễn Thị Quỳnh та ін. "NGHIÊN CỨU KHẢ NĂNG KHÁNG VI KHUẨN Streptococus agalactiae CỦA CÁC LOẠI CAO CHIẾT THẢO DƯỢC TRÊN CÁ RÔ PHI (Oreochromis sp.)". Tạp chí Khoa học và công nghệ nông nghiệp Trường Đại học Nông Lâm Huế 7, № 1 (2023): 3452–64. http://dx.doi.org/10.46826/huaf-jasat.v7n1y2023.1033.

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Nghiên cứu đánh giá khả năng kháng các chủng vi khuẩn Streptococcus agalactiae thuộc kiểu huyết thanh III và Ib trên cá rô phi của một số loại cao chiết thảo dược: tía tô (Perilla frutescens), kinh giới (Elsholtzia ciliata), bạch chỉ (Angelica dahurica), xuyên tâm liên (Andrographis paniculata), cỏ mực (Eclipta prostrata), cỏ gà (Cynodon dactylon), sài đất (Wedelia chinensis), mướp đắng (Momordica charantia). Các loại cao chiết được chiết xuất trong 3 loại dung môi: nước cất, ethanol 96% và methanol 99,8%, được xử lý nhiệt và cô quay để loại bỏ dung môi. Kết quả đánh giá khả năng kháng khuẩn c
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Piccinelli, Giorgio, Prabhavathi Fernandes, Carlo Bonfanti, Francesca Caccuri, Arnaldo Caruso, and Maria Antonia De Francesco. "In VitroActivity of Solithromycin against Erythromycin-Resistant Streptococcus agalactiae." Antimicrobial Agents and Chemotherapy 58, no. 3 (2013): 1693–98. http://dx.doi.org/10.1128/aac.02210-13.

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ABSTRACTThein vitroantibacterial activity of solithromycin (CEM-101) against macrolide-resistant isolates (n= 62) ofStreptococcus agalactiae(group B streptococcus [GBS]) was determined. Phenotypic characterization of macrolide-resistant strains was performed by double-disc diffusion testing. A multiplex PCR was used to identify theerm(B),erm(TR), andmef(A/E) genes, capsular genotypes, and alpha-like (Alp) protein genes from the GBS strains. Determination of MIC was carried out using the microdilution broth method. The Etest method was used for penicillin, azithromycin, clarithromycin, and eryt
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Pfaller, Michael A., Robert K. Flamm, Ronald N. Jones, David J. Farrell, and Rodrigo E. Mendes. "Activities of Tedizolid and Linezolid Determined by the Reference Broth Microdilution Method against 3,032 Gram-Positive Bacterial Isolates Collected in Asia-Pacific, Eastern Europe, and Latin American Countries in 2014." Antimicrobial Agents and Chemotherapy 60, no. 9 (2016): 5393–99. http://dx.doi.org/10.1128/aac.00881-16.

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ABSTRACTTedizolid and linezolidin vitroactivities against 3,032 Gram-positive pathogens collected in Asia-Pacific, Eastern European, and Latin American medical centers during 2014 were assessed. The isolates were tested for susceptibility by the current reference broth microdilution methods. Due to concern over the effect of MIC endpoint criteria on the results of testing the oxazolidinones tedizolid and linezolid, MIC endpoint values were read by two methods: (i) reading the MIC at the first well where the trailing began without regard for pinpoint trailing, according to CLSI M07-A10 and M100
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37

Rahchamani, Reza, Saman Zarooni, and Matia Sadat Borhani. "The Chemical Composition and Antibacterial Effect of Essential Oils of Rosemary and Basil in Milk." Iranian Journal of Veterinary Medicine 19, no. 03 (2025): 539–48. https://doi.org/10.32598/ijvm.19.3.1005517.

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Background: According to the diverse side effects of antibiotics, new and natural antibacterial substances are needed to treat bacterial diseases, and one of these substances is the essential oils (EOs) of medicinal plants. Milk fat and protein may reduce the antimicrobial impact of EOs. Objectives: This study aims to investigate the antibacterial activity of rosemary and basil EOs compared to lincospectinomycin antibiotic against three mastitis-causing bacteria, Streptococcus agalactiae, Staphylococcus aureus, and Escherichia coli in milk media. Methods: Chemical compounds in EOs were identif
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38

Tran, Thi My Duyen, Trong Tuan Nguyen, and Thi Tuyet Hoa Tran. "In vitro antibacterial activity of several plant extracts against fish bacterial pathogens." Can Tho University Journal of Science 13, Aquaculture (2021): 106–12. http://dx.doi.org/10.22144/ctu.jen.2021.023.

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Crude methanol extract of 9 Vietnamese plants were in vitro screened for their antibacterial activity against three common freshwater fish pathogens including Aeromonas hydrophila, Edwardsiella ictaluri, and Streptococcus agalactiae. Agar disc diffusion method was used to evaluate the antibacterial activity, then solvent extract was performed for the extracts which exhibited the strongest and a broad-spectrum antibacterial activity. Minimal inhibitory concentration (MIC) was conducted for effective plant extracts using broth dilution method. The results indicated that most of the plant extract
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39

Yanik, Keramettin, Emin Guluzade, Kemal Bilgin, et al. "Ceftaroline activity on certain respiratory tract and wound infection agents at the minimum inhibitory concentration level." Journal of Infection in Developing Countries 9, no. 10 (2015): 1086–90. http://dx.doi.org/10.3855/jidc.6300.

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Introduction: The aim of this study was to investigate the effectiveness of ceftaroline against agents frequently isolated from respiratory tract and wound infections. Methodology: The study included a total of 250 strains isolated from various clinical specimens, among which were Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus dysagalactiae, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catharralis. The bacteria were identified using the matrix-assisted laser desorption/ionization time-of-flight method and conventional methods. The bacteria’s antibiotic susce
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40

Karpiński, Tomasz M., and Artur Adamczak. "Fucoxanthin—An Antibacterial Carotenoid." Antioxidants 8, no. 8 (2019): 239. http://dx.doi.org/10.3390/antiox8080239.

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Fucoxanthin is a carotenoid produced by brown algae and diatoms. This compound has several biological properties such as antioxidant, anti-obesity, anti-diabetic, anticancer, and antimicrobial activities. Unfortunately, until now the latter effect has been poorly confirmed. The aim of this study was an evaluation of fucoxanthin activity against 20 bacterial species. Antimicrobial effect of fucoxanthin was determined by using the agar disc-diffusion and micro-dilution methods. The studied carotenoid acted against 13 bacteria growing in aerobic conditions. It was observed to have a significantly
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41

Mbarga, Manga J. A., Razan Marouf, Irina V. Podoprigora, Kitio L. D. Anyutoulou, Yuri V. Chapurin, and Irina N. Sharova. "Long exposure impact of antibiotics subinhibitory doses and silver nanoparticles on uropathogenic bacteria." RUDN Journal of Medicine 27, no. 3 (2023): 391–402. http://dx.doi.org/10.22363/2313-0245-2023-27-3-391-402.

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Relevance. Although the primary purpose of using antibiotics is to treat infectious diseases, their misuse gradually leads to loss of their effectiveness. The aim of the current investigation was to explore the changes that occur in uropathogenic bacteria after long exposure to antimicrobials. Materials and Methods. We compared the effects of long-term exposure to ampicillin, cefazoline, kanamycin and silver nanoparticles (AgNPs) on susceptibility, biofilm formation and planktonic bacteria in 4 clinical uropathogenic strains namely Escherichia coli (UPEC), Staphylococcus aureus (S. aureus), En
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42

Sela, Floresha, Marija Karapandzova, Gjose Stefkov, et al. "Chemical composition and antimicrobial activity of leaves essential oil of Juniperus communis (Cupressaceae) grown in Republic of Macedonia." Macedonian Pharmaceutical Bulletin 59 (June 2013): 23–32. http://dx.doi.org/10.33320/maced.pharm.bull.2013.59.003.

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Chemical composition and antimicrobial activity of essential oils isolated from leaves of three different samples of wild growing Juniperus communis L. (Cupressaceae) from R. Macedonia was investigated. Essential oil yield ranged from 7.3 to 9.0 ml/kg. Performing GC/ FID/MS analysis, ninety components were identified, representing 86.07-93.31% of the oil. The major components of the leaves essential oil (LEO) were α-pinene (21.37-28.68%) and sabinene (2.29-16.27%), followed by limonene, terpinen-4-ol, β-elemene, trans-(E)-caryophyllene, germacrene D and δ-cadinene. Antimicrobial screening of t
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43

Bolte, Josef, Yanchao Zhang, Nicole Wente, and Volker Krömker. "In Vitro Susceptibility of Mastitis Pathogens Isolated from Clinical Mastitis Cases on Northern German Dairy Farms." Veterinary Sciences 7, no. 1 (2020): 10. http://dx.doi.org/10.3390/vetsci7010010.

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The present research study investigated the susceptibility of common mastitis pathogens—obtained from clinical mastitis cases on 58 Northern German dairy farms—to routinely used antimicrobials. The broth microdilution method was used for detecting the Minimal Inhibitory Concentration (MIC) of Streptococcus agalactiae (n = 51), Streptococcus dysgalactiae (n = 54), Streptococcus uberis (n = 50), Staphylococcus aureus (n = 85), non-aureus staphylococci (n = 88), Escherichia coli (n = 54) and Klebsiella species (n = 52). Streptococci and staphylococci were tested against cefquinome, cefoperazone,
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Ferreira Eduardo da Costa, Andréia, João Alfredo Moraes, Jessica Silva Santos de Oliveira, et al. "Reactive oxygen species involved in apoptosis induction of human respiratory epithelial (A549) cells by Streptococcus agalactiae." Microbiology 162, no. 1 (2016): 94–99. http://dx.doi.org/10.1099/mic.0.000202.

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Adikesavalu, Harresh, Sayani Banerjee, Avijit Patra, and Thangapalam Jawahar Abraham. "Meningoencephalitis in farmed monosex Nile tilapia (Oreochromis niloticus L.) caused by Streptococcus agalactiae." Archives of Polish Fisheries 25, no. 3 (2017): 187–200. http://dx.doi.org/10.1515/aopf-2017-0018.

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Abstract Aquaculture of tilapia is a new research venture in India. With intensification in farming practices, tilapia are increasingly susceptible to bacterial infections. This article describes the isolation and identification of pathogenic bacteria from cultured monosex Nile tilapia, Oreochromis niloticus (L.), that experienced moderate to severe mortalities in West Bengal, India between September and August 2014 and histopathological alterations in various organs. Gram-positive diplococci, identified as Streptococcus agalactiae with Streptococcus identification kits and 16S rDNA sequencing
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46

Dagne, Abebe, Sileshi Degu, Abiy Abebe, and Daniel Bisrat. "Antibacterial Activity of a Phenylpropanoid from the Root Extract of Carduus leptacanthus Fresen." Journal of Tropical Medicine 2023 (September 6, 2023): 1–6. http://dx.doi.org/10.1155/2023/4983608.

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Background. The emergence and rapid spread of antimicrobial drug-resistance microorganisms exacerbate the treatment of infectious diseases, underscoring the importance of finding new, safe, and effective drugs. In Ethiopia, the roots of Carduus leptacanthus have traditionally been employed to treat microbial infectious diseases The aim of this study was to evaluate the antibacterial activity of the root extract and its primary components against six bacterial strains (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus agalactiae, Escherichia coli, Pseudomonas aeruginosa, and Kleb
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47

Sutcliffe, Iain C., Gary W. Black, and Dean J. Harrington. "Bioinformatic insights into the biosynthesis of the Group B carbohydrate in Streptococcus agalactiae." Microbiology 154, no. 5 (2008): 1354–63. http://dx.doi.org/10.1099/mic.0.2007/014522-0.

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Sharon, Joshi, Thangapalam Jawahar Abraham, Arya Sen, et al. "Effects of Streptococcus agalactiae infection and oral florfenicol administration on the hemato-biochemistry, erythrocyte morphology and histopathology of Oreochromis niloticus." Journal of Fisheries 13, no. 1 (2025): 131209. https://doi.org/10.17017/j.fish.720.

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Streptococcosis is considered one of the most important diseases affecting farmed tilapia, causing severe economic losses. Antimicrobial therapy is the principal control measure applied during outbreaks. This research assessed the efficacy of florfenicol (FFC) when fed at 15 mg kg biomass–1 day–1 for 10 days against Streptococcus agalactiae infection in Oreochromis niloticus in terms of survival, changes in hemato-biochemistry, erythro-morphology, and histoarchitecture of the vital organs. Streptococcus agalactiae was moderately virulent to O. niloticus with a lethal dose (LD50) of 1.26×108 ce
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Chen, Ko-Hung, Yu-Tsung Huang, Chun-Hsing Liao, Wang-Hui Sheng, and Po-Ren Hsueh. "In VitroActivities of Tedizolid and Linezolid against Gram-Positive Cocci Associated with Acute Bacterial Skin and Skin Structure Infections and Pneumonia." Antimicrobial Agents and Chemotherapy 59, no. 10 (2015): 6262–65. http://dx.doi.org/10.1128/aac.00390-15.

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ABSTRACTTedizolid is a novel, expanded-spectrum oxazolidinone with potent activity against a wide range of Gram-positive pathogens. A total of 425 isolates of Gram-positive bacteria were obtained consecutively from patients with acute bacterial skin and skin structure infections (ABSSSIs) or pneumonia. These isolates included methicillin-susceptibleStaphylococcus aureus(MSSA) (n= 100), methicillin-resistantStaphylococcus aureus(MRSA) (n= 100),Streptococcus pyogenes(n= 50),Streptococcus agalactiae(n= 50),Streptococcus anginosusgroup (n= 75),Enterococcus faecalis(n= 50), and vancomycin-resistant
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Desmond, Anna, Fiona O’Halloran, Lesley Cotter, Colin Hill, and Des Field. "Bioengineered Nisin A Derivatives Display Enhanced Activity against Clinical Neonatal Pathogens." Antibiotics 11, no. 11 (2022): 1516. http://dx.doi.org/10.3390/antibiotics11111516.

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Neonatal infection is a significant cause of mortality and morbidity in infants. The global incidence of multi-drug resistance continues to rise among neonatal pathogens, indicating a need for alternative treatment strategies. Nisin is an antimicrobial peptide that exhibits broad-spectrum activity against a wide variety of clinical pathogens and can be used in combination with antibiotics to improve their effectiveness. This study examined the activity of nisin and bioengineered derivatives against multi-drug resistant Streptococcus agalactiae and Staphylococcus capitis isolates and investigat
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