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Artykuły w czasopismach na temat „Stro1”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 1 lutego 2022

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1

Francois, Sabine, Yizhuo Zhang, Morad Bensidhoum, Christelle Mazurier, Aisha Nasef, Sandrine Bouchet, Noelle Mathieu i in. "Stro-1 Positive and Stro-1 Negative Human Mesenchymal Stem Cells Express Different Levels of Immunosuppression." Blood 106, nr 11 (16.11.2005): 2305. http://dx.doi.org/10.1182/blood.v106.11.2305.2305.

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Abstract Mesenchymal stem cells (MSC) have been shown to elicit immunosuppressive effect on allogeneic lymphocyte response. However, MSC are heterogeneous and data on the inhibitory abilities of different MSC subsets are lacking. The Stro-1 antigen potentially defines a more pure and more primitive MSC subset We compared the suppressive property of expanded Stro-1+ and Stro-1− MSC when add to mixed lymphocyte reactions (MLR) or to mitogen response assays. Results showed that T lymphocyte proliferation was significantly more inhibited by expanded Stro-1+ (11.0%~63.7% of maximal response) than by expanded stro-1− MSC (35.5%~106% of maximal response) (p<0.01). Results indicated that as few as 1,000 expanded Stro-1+ MSC could induce a similar inhibition of lymphocyte proliferation than 10 times more (10,000 cells) expanded Stro-1− MSC −. Stro-1+ MSC was the main population responsible for T cell inhibition. The expression of genes coding for inhibitors of T-cell activation and proliferation (IL-10, LIF, IDO, TGF-b1, HGF, HLA-G), for adhesion molecules (VCAM, LFA-3), and for chemotactic factors (IL-8, SDF-1) was analysed by semi-quantitative real time RT-PCR in the Stro1+ and Stro1- MSC. Results are expressed as a fold increase in the mRNA level in expanded Stro-1+ compared to Stro-1− MSC. IL-10 (0.24 fold p=0.002) and TGF-β1 (0.43 fold, p=0.03) were down regulated in expanded Stro-1+ compared to Stro-1− MSC. LIF (106 fold, p=0.025), IDO (6130 fold, p=0.04), HGF (24 fold, p=0.01), and HLA-G (260 fold, p= 0.02) were up regulated in expanded Stro-1+ compared to Stro-1− MSC. VCAM-1 and LFA-3 were up regulated in expanded Stro-1+ compared to Stro-1− MSC (28.2 fold, p=0.03 and 298.4 fold, p=0.04 for VCAM-1 and LFA-3 respectively): IL-8 was over expressed in expanded Stro-1+ (4.9 fold, p=0.0015). There was no significant difference between expanded Stro-1+ and Stro-1− MSC for SDF-1. These results suggest that LIF, IDO, HGF, HLA-G inhibitory factors and VCAM1, LFA-3 adhesion molecules are mainly responsible for the different T cell inhibition observed between Stro-1+ and Stro-1− MSC. These findings suggest that in clinics a Stro-1+ pre-selection of MSC might produce a more effective immunosuppression especially for the prevention and the treatment of graft versus host disease.

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Mas, Aymara, Lauren Prusinski, Qiwei Yang, Patricia Diaz-Gimeno, Lelyand Stone, Michael P. Diamond, Carlos Simón i Ayman Al-Hendy. "Role of Stro1+/CD44+ stem cells in myometrial physiology and uterine remodeling during pregnancy†". Biology of Reproduction 96, nr 1 (23.12.2016): 70–80. http://dx.doi.org/10.1095/biolreprod.116.143461.

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Choi, S. A., J. H. Lee, K. J. Kim, E. Y. Kim, K. S. Park, Y. B. Park, X. Li, Y. N. Ha, J. Y. Park i M. K. Kim. "291 ISOLATION AND CHARACTERIZATION OF MESENCHYMAL STEM CELLS DERIVED FROM HUMAN AMNIOTIC FLUID". Reproduction, Fertility and Development 23, nr 1 (2011): 243. http://dx.doi.org/10.1071/rdv23n1ab291.

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Adult stem cells have the capacity to differentiate into several different cell types, although their differentiation potential is limited compared with that of embryonic stem cells. Thus, adult stem cells are regarded as an exciting source for new cell therapies. Recent observations also indicate that stem cells derived from second-trimester amniocentesis are pluripotent – capable of differentiating into multiple lineages, including representatives of all 3 embryonic germ layers. In addition, amniotic fluid stem cells can be used in the generation of disease- or patient-specific stem cells, and amniotic fluid stem cells could be an ideal source for autologous cell replacement therapy in the later life of the fetus. The aim of the present study was to investigate isolation and characterisation of human amniotic fluid-derived mesenchymal stem cells (hAFS). We successfully isolated and characterised hAFS. Amniotic fluid samples were collected in the second trimester (median gestational age: 16 weeks, range: 15–17 weeks) for prenatal diagnosis. Specimens (2 mL) were centrifuged and incubated in low-glucose DMEM supplemented with 10% FBS, 25 ng of basic fibroblast growth factor, and 10 ng of epidermal growth factor at 37°C with 5% CO2. Human amniotic fluid cell (passage 6) expression of stem cell specific markers OCT-4, SOX2, Rex1, FGF4, and NANOG was confirmed by RT-PCR. Flow cytometric analysis showed that hAFS (passage 10) were positive for CD44, CD29, CD146, STRO1, and CD90 but negative for CD19. Immunocytochemical analysis of hAFS (passage 11) also showed the expression of OCT-4, SSEA-1, CD44, CD29, CD146, STRO1, and CD90, but hAFS were negative for CD19 and CD14. In conclusion, according to the previous studies on other mammalians, hAFS are an appropriate source of pluripotent stem cells. Here, we demonstrated that hAFS have a high expression of stem cell specific marker, including embryonic stem cell marker and mesenchymal stem cell marker. Therefore, amniotic fluid may be a suitable alternative source of multipotent stem cells.

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Mokhtari, Saloomeh, Evan Joseph Colletti, Chad Sanada, Zanetta S. Lamar, Paul J. Simmons, Anthony Atala, Diane S. Krause, Esmail D. Zanjani, Christopher D. Porada i Graca Almeida-Porada. "A Human Bone Marrow-Derived Stromal Cell Population with Hemogenic Potential". Blood 126, nr 23 (3.12.2015): 1201. http://dx.doi.org/10.1182/blood.v126.23.1201.1201.

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Abstract During ontogeny, definitive hematopoietic stem/progenitor cells (HSC) are thought to arise from vascular endothelial cells, through an endothelial-to-hematopoietic transition, a natural process that occurs in unique, specialized embryonic hemogenic endothelial cells. Developmental studies, and experiments using pluripotent stem cells in an effort to recapitulate this process and thereby gain a better understanding of the emergence of definitive hematopoiesis, have collectively led to the prevailing view that the hemogenic endothelium constitutes a transient population of cells within the embryo that rapidly disappears during development and is absent in the adult. Herein, we provide the first evidence that at early time points of gestation, prior to the establishment of hematopoiesis, a unique subpopulation of Stro-1+ cells present within the inner part of the developing human bone marrow co-expresses APLNR, a marker of angiogenic mesoderm. Moreover, these Stro-1+APLNR+ cells express multiple other markers described for hemogenic endothelium, and subsequently contribute to the vasculature, cartilage, and bone. Importantly, we also show that cells expressing these same markers of primitive mesoderm/hemogenic endothelium persist at low frequency within the adult marrow. These adult-derived cells can be extensively expanded in vitro without loss of potential, but lack hematopoietic colony-forming potential in vitro. However, upon transplantation into a fetal microenvironment, clonally-derived populations of these adult Stro1+ isolated stromal progenitors (SIPs) not only contribute to the vasculature and nascent BM niches, but also efficiently generate, at a clonal level, hematopoietic stem cells (HSC) that are capable of robust, multilineage hematopoietic reconstitution, with generation of both myeloid and lymphoid cells upon serial transplantation. In conclusion, our studies have thus uncovered the latent potential of a highly expandable population of seemingly vestigial adult human somatic cells, whose ontogenic history includes a phenotype identical to that described for hemogenic endothelium. We have also shown that, if provided with the appropriate/necessary inductive factors, these unique adult cells are capable of giving rise to hematopoietic cells that fulfill the gold standard criteria for bona fide HSC. Therefore, these cells could potentially be more amenable to reprogramming technologies, to produce HSC that could be used to treat/cure a broad variety of blood diseases. Disclosures No relevant conflicts of interest to declare.

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Leible, Ludwig, S. Kälber i Gunnar Kappler. "Kraftstoff, Strom und Wärme aus Stroh und Waldrestholz". TATuP - Zeitschrift für Technikfolgenabschätzung in Theorie und Praxis 16, nr 3 (1.11.2007): 94–97. http://dx.doi.org/10.14512/tatup.16.3.94.

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Guzmán-Uribe, Daniela, Keila Neri Alvarado Estrada, Amaury de Jesús Pozos Guillén, Silvia Martín Pérez i Raúl Rosales Ibáñez. "Development of A Three-Dimensional Tissue Construct from Dental Human Ectomesenchymal Stem Cells: In Vitro and In Vivo Study". Open Dentistry Journal 6, nr 1 (28.12.2012): 226–34. http://dx.doi.org/10.2174/1874210601206010226.

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Application of regenerative medicine technology provides treatment for patients with several clinical problems, like loss of tissue and its function. The investigation of biological tooth replacement, dental tissue engineering and cell culture, scaffolds and growth factors are considered essential. Currently, studies reported on the making of threedimensional tissue constructs focused on the use of animal cells in the early stages of embryogenesis applied to young biomodels. The purpose of this study was the development and characterization of a three-dimensional tissue construct from human dental cells. The construct was detached, cultured and characterized in mesenchymal and epithelial cells of a human tooth germ of a 12 year old patient. The cells were characterized by specific membrane markers (STRO1, CD44), making a biocomplex using Pura Matrix as a scaffold, and it was incubated for four days and transplanted into 30 adult immunosuppressed male Wistar rats. They were evaluated at 6 days, 10 days and 2 months, obtaining histological sections stained with hematoxylin and eosin. Cell cultures were positive for specific membrane markers, showing evident deviations in morphology under phase contrast microscope. Differentiation and organization were noted at 10 days, while the constructs at 2 months showed a clear difference in morphology, organization and cell type. It was possible to obtain a three-dimensional tissue construct from human dental ectomesenchymal cells achieving a degree of tissue organization that corresponds to the presence of cellular stratification and extracellular matrix.

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Leible, Ludwig, S. Kälber i Gunnar Kappler. "Kraftstoff, Wärme oder Strom aus Stroh und Waldrestholz – ein systemanalytischer Vergleich". TATuP - Zeitschrift für Technikfolgenabschätzung in Theorie und Praxis 15, nr 1 (1.04.2006): 61–72. http://dx.doi.org/10.14512/tatup.15.1.61.

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Elkafas, Hoda, Mohamed Ali, Engy Elmorsy, Rehab Kamel, Winston E. Thompson, Osama Badary, Ayman Al-Hendy i Qiwei Yang. "Vitamin D3 Ameliorates DNA Damage Caused by Developmental Exposure to Endocrine Disruptors in the Uterine Myometrial Stem Cells of Eker Rats". Cells 9, nr 6 (12.06.2020): 1459. http://dx.doi.org/10.3390/cells9061459.

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Early-life exposure of the myometrium to endocrine-disrupting chemicals (EDCs) has been shown to increase the risk of uterine fibroid (UF) prevalence in adulthood. Vitamin D3 (VitD3) is an unique, natural compound that may reduce the risk of developing UFs. However, little is known about the role and molecular mechanism of VitD3 on exposed myometrial stem cells (MMSCs). We investigated the role and molecular mechanism underlying VitD3 action on DNA damage response (DDR) defects in rat MMSCs due to developmental exposure to diethylstilbestrol (DES), with the additional goal of understanding how VitD3 decreases the incidence of UFs later in life. Female newborn Eker rats were exposed to DES or a vehicle early in life; they were then sacrificed at 5 months of age (pro-fibroid stage) and subjected to myometrial Stro1+/CD44+ stem cell isolation. Several techniques were performed to determine the effect of VitD3 treatment on the DNA repair pathway in DES-exposed MMSCs (DES-MMSCs). Results showed that there was a significantly reduced expression of RAD50 and MRE11, key DNA repair proteins in DES-exposed myometrial tissues, compared to vehicle (VEH)-exposed tissues (p < 0.01). VitD3 treatment significantly decreased the DNA damage levels in DES-MMSCs. Concomitantly, the levels of key DNA damage repair members, including the MRN complex, increased in DES-MMSCs following treatment with VitD3 (p < 0.01). VitD3 acts on DNA repair via the MRN complex/ATM axis, restores the DNA repair signaling network, and enhances DDR. This study demonstrates, for the first time, that VitD3 treatment attenuated the DNA damage load in MMSCs exposed to DES and classic DNA damage inducers. Moreover, VitD3 targets primed MMSCs, suggesting a novel therapeutic approach for the prevention of UF development.

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Chmilewsky, F., R. Liang, M. Kanazawa, I. About, L. F. Cooper i A. George. "C5L2 Regulates DMP1 Expression during Odontoblastic Differentiation". Journal of Dental Research 98, nr 5 (31.01.2019): 597–604. http://dx.doi.org/10.1177/0022034518820461.

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The presence of stem cells within the dental-pulp tissue as well as their differentiation into a new generation of functional odontoblast-like cells constitutes an important step of the dentin-pulp regeneration. Recent investigations demonstrated that the complement system activation participates in 2 critical steps of dentin-pulp regeneration: pulp progenitor’s recruitment and pulp nerve sprouting. Surprisingly, its implication in odontoblastic differentiation has not been addressed yet. Since the complement receptor C5a receptor-like 2 (C5L2) is expressed by different stem cells, the aim of this study is to investigate if the dental pulp stem cells express C5L2 and if this receptor participates in odontoblastic differentiation. Immunohistochemistry performed on human third molar pulp sections showed a perivascular co-localization of the mesenchymal stem cell markers STRO1 and C5L2. In vitro immunofluorescent staining confirmed that hDPSCs express C5L2. Furthermore, we determined by real-time polymerase chain reaction that the expression of C5L2 is highly modulated in human dental pulp stem cells (hDPSCs) undergoing odontoblastic differentiation. Moreover, we showed that this odontogenesis-regulated expression of C5L2 is specifically potentiated by the proinflammatory cytokine TNFα. Using a C5L2-siRNA silencing strategy, we provide direct evidence that C5L2 constitutes a negative regulator of the dentinogenic marker DMP1 (dentin matrix protein 1) expression by hDPSCs. Our findings suggest a direct correlation between the odontoblastic differentiation and the level of C5L2 expression in hDPSCs and identify C5L2 as a negative regulator of DMP1 expression by hDPSCs during the odontoblastic differentiation and inflammation processes. This work is the first to demonstrate the involvement of C5L2 in the biological function of stem cells, provides an important knowledge in understanding odontoblastic differentiation of dental pulp stem cells, and may be useful in future dentin-pulp engineering strategies.

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Peraldo-Neia, Caterina, Annamaria Massa, Francesca Vita, Marco Basiricò, Chiara Raggi, Paola Bernabei, Paola Ostano i in. "A Novel Multidrug-Resistant Cell Line from an Italian Intrahepatic Cholangiocarcinoma Patient". Cancers 13, nr 9 (23.04.2021): 2051. http://dx.doi.org/10.3390/cancers13092051.

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Chemotherapy resistance is a relevant clinical issue in tumor treatment, in particular in biliary tract carcinoma (BTC), for which there are no effective therapies, neither in the first nor in the second line. The development of chemoresistant cell lines as experimental models to investigate the mechanisms of resistance and identify alternative druggable pathways is mandatory. In BTC, in which genetics and biological behavior depend on the etiology, ethnicity, and anatomical site of origin, the creation of models that better recapitulate these characteristics is even more crucial. Here we have established and characterized an intrahepatic cholangiocarcinoma (iCCA) cell line derived from an Italian patient, called 82.3. Cells were isolated from a patient-derived xenograft (PDX) and, after establishment, immunophenotypic, biological, genetic, molecular characteristics, and tumorigenicity in vivo in NOD/SCID mice were investigated. 82.3 cells exhibited epithelial morphology and cell markers (EPCAM, CK7, and CK19); they also expressed different cancer stem markers (CD44, CD133, CD49b, CD24, Stro1, PAX6, FOXA2, OCT3/4), α–fetoprotein and under anchorage-independent and serum-free conditions were capable of originating cholangiospheres. The population doubling time was approximately 53 h. In vitro, they demonstrated a poor ability to migrate; in vivo, 82.3 cells retained their tumorigenicity, with a long latency period (16 weeks). Genetic identity using DNA fingerprinting analysis revealed 16 different loci, and the cell line was characterized by a complex hyperdiploid karyotype. Furthermore, 82.3 cells showed cross-resistance to gemcitabine, 5-fluorouracil, carboplatin, and oxaliplatin; in fact, their genetic profile showed that 60% of genes (n = 168), specific for drug resistance and related to the epithelial-mesenchymal transition, were deregulated in 82.3 cells compared to a control iCCA cell line sensitive to chemotherapeutics. RNA sequencing analysis revealed the enrichment for genes associated with epithelial to mesenchymal transition (EMT), vasculature development, and extracellular matrix (ECM) remodeling, underlining an aggressive phenotype. In conclusion, we have created a new iCCA cell line of Caucasian origin: this could be exploited as a preclinical model to study drug resistance mechanisms and to identify alternative therapies to improve the prognosis of this tumor type.

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Janel, Alexandre, Nathalie Boiret-Dupré, Juliette Berger, Céline Bourgne, Richard Lemal, Frédérique Dubois-Galopin, Pierre Déchelotte i in. "Bone Marrow Cell Aggregates: A Way of Collecting the Adult Human Hematopoietic Stem Cell Niche". Blood 124, nr 21 (6.12.2014): 4363. http://dx.doi.org/10.1182/blood.v124.21.4363.4363.

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Abstract Hematopoietic stem cell (HSC) function is critical in maintaining hematopoiesis continuously throughout the lifespan of an organism and any change in their ability to self-renew and/or to differentiate into blood cell lineages induces severe diseases. Postnatally, HSC are mainly located in bone marrow where their stem cell fate is regulated through a complex network of local influences, thought to be concentrated in the bone marrow (BM) niche. Despite more than 30 years of research, the precise location of the HSC niche in human BM remains unclear because most observations were obtained from mice models. BM harvesting collects macroscopic coherent tissue aggregates in a cell suspension variably diluted with blood. The qualitative interest of these tissue aggregates, termed hematons, was already reported (first by I. Blaszek's group (Blaszek et al., 1988, 1990) and by our group (Boiret et al., 2003)) yet they remain largely unknown. Should hematons really be seen as elementary BM units, they must accommodate hematopoietic niches and must be a complete ex vivo surrogate of BM tissue. In this study, we analyzed hematons as single tissue structures. Biological samples were collected from i) healthy donor bone marrow (n= 8); ii) either biological samples collected for routine analysis by selecting bone marrow with normal analysis results (n=5); or iii) from spongy bone collected from the femoral head during hip arthroplasty (n=4). After isolation of hematons, we worked at single level, we used immunohistochemistry techniques, scanning electronic microscopy, confocal microscopy, flow cytometry and cell culture. Each hematon constitutes a miniature BM structure organized in lobular form around the vascular tree. Hematons are organized structures, supported by a network of cells with numerous cytoplasmic expansions associated with an amorphous structure corresponding to the extracellular matrix. Most of the adipocytes are located on the periphery, and hematopoietic cells can be observed as retained within the mesenchymal network. Although there is a degree of inter-donor variability in the cellular contents of hematons (on average 73 +/- 10 x103 cells per hematon), we observed precursors of all cell lines in each structure. We detected a higher frequency of CD34+ cells than in filtered bone marrow, representing on average 3% and 1% respectively (p<0.01). Also, each hematon contains CFU-GM, BFU-E, CFU-Mk and CFU-F cells. Mesenchymal cells are located mainly on the periphery and seem to participate in supporting the structure. The majority of mesenchymal cells isolated from hematons (21/24) sustain in vitro hematopoiesis. Interestingly, more than 90% of the hematons studied contained LTC-ICs. Furthermore, when studied using confocal microscopy, a co-localization of CD34+ cells with STRO1+ mesenchymal cells was frequently observed (75% under 10 µm of the nearest STRO-1+ cell, association statistically highly significant; p <1.10-16). These results indicate the presence of one or several stem cell niches housing highly primitive progenitor cells. We are confirming these in vitro data with an in vivo xenotransplantation model. These structures represent the elementary functional units of adult hematopoietic tissue and are a particularly attractive model for studying homeostasis of the BM niche and the pathological changes occurring during disease. Disclosures No relevant conflicts of interest to declare.

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Winterhagen, Johannes. "Strom an, Strom aus". VDI nachrichten 75, nr 21 (2021): 13. http://dx.doi.org/10.51202/0042-1758-2021-21-13.

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Balqis, Dafina, Yudhi Adrianto i Jongky Hendro Prayitno. "HBA1C LEVELS IN TYPE 2 DIABETES MELLITUS PATIENTS WITH AND WITHOUT INCIDENCE OF THROMBOTIC STROKE (Kadar HbA1c Pasien Diabetes Melitus Tipe 2 dengan dan Tanpa Kejadian Strok Infark Trombotik)". INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 23, nr 1 (11.04.2018): 56. http://dx.doi.org/10.24293/ijcpml.v23i1.1185.

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Strok saat ini menjadi salah satu penyebab utama kematian global. Hubungan antara kejadian strok dengan diabetes telahlama diketahui. Kontrol gula darah, yang dipantau melalui kadar HbA1c, telah menunjukkan hubungan dengan strok dan penyakitkardiovaskular lain. Kajian ini untuk menentukan perbedaan kadar HbA1c antara pasien diabetes melitus tipe 2 dengan dan tanpakejadian strok infark trombotik. Metode penelitian yang digunakan adalah analisis retrospektif menggunakan rekam medis pasienselama 3,5 tahun. Penelitian ini mengumpulkan data kadar HbA1c dari 443 pasien diabetes melitus tipe 2 kemudian membandingkanrerata kadar HbA1c antara pasien diabetes mellitus tipe 2 dengan kejadian strok trombotik (n=74) dan tanpa kejadian strok trombotik(n=369). Perbandingan tingkat HbA1c juga dilakukan secara terpisah antara laki-laki dan perempuan. Kajian ini menemukan reratakadar HbA1c yang tinggi di kedua kelompok sampel (10,49%±2,53% untuk kelompok dengan kejadian strok infark trombotik dan10,44%±2,8% untuk kelompok tanpa kejadian strok infark trombotik) dengan perbandingan sarana p>0,05. Perbandingan yangdilakukan secara terpisah di laki-laki dan perempuan juga menunjukkan hasil yang sama dengan p>0,05. Sebagai simpulan, kadarHbA1c di kedua kelompok penelitian sama-sama tinggi dan tidak ada perbedaan bermakna kadar HbA1c yang ditemukan di pasiendengan diabetes tipe 2 dengan dan tanpa kejadian strok trombotik.

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Hofstätter, Reinhard. "Haustechnik 2.0 – „Kabel statt Rohre“". HLH 70, nr 02 (2019): 99–100. http://dx.doi.org/10.37544/1436-5103-2019-02-99.

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Warmwasser aus Strom war lange Zeit verpönt. Strom war zu wertvoll zum Verheizen. Aber die Fachwelt hat es bereits erkannt: Günstiger Photovoltaik-Strom ermöglicht ein Revival dezentraler elektrischer Warmwasserspeicher im Wohnungsbau. Dieser Artikel gibt Einblicke in die Power-to-Heat Technologie, die das Erwärmen von Wasser mit Strom zur effizienten Energienutzung macht.

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Tursina, Alya, Widayanti Widayanti i Ariko Rahmat Putra. "Hubungan Letak Lesi Strok Iskemik dengan Kualitas Tidur di RSAU dr. M. Salamun Bandung". Jurnal Integrasi Kesehatan & Sains 1, nr 2 (31.07.2019): 127–30. http://dx.doi.org/10.29313/jiks.v1i2.4637.

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Prevalensi strok di Indonesia terus meningkat seiring dengan usia harapan hidup yang semakin meningkat dan menyebabkan banyak kematian. Pasien strok terutama strok iskemik dapat mengalami berbagai macam gangguan tidur. Macam gangguan tidur pada penderita strok bergantung pada defisit neurologis yang dialaminya. Tujuan penelitian ini mengetahui hubungan lokasi lesi strok iskemik daerah kortikal dan subkortikal dengan kualitas tidur menggunakan pemeriksaan Pittsburgh Sleep Quality Index (PSQI) di RSAU dr. M. Salamun Bandung. Penelitian ini merupakan penelitian observasional dengan rancangan penelitian potong lintang. Penelitian dilakukan terhadap 38 penderita yang pertama kali didiagnosis strok iskemik yang dirawat inap di Bagian Neurologi RSAU dr. M. Salamun Bandung pada bulan Juni sampai Oktober 2016. Instrumen penelitian berupa kuesioner PSQI yang dilakukan pada hari ke-7 pasien dirawat inap. Statistical for Social Science (SPSS) versi 17 digunakan untuk mengolah data. Hasil penelitian menunjukkan terdapat hubungan bermakna lokasi lesi strok iskemik daerah kortikal dan subkortikal dengan kualitas tidur (p=0,215). Penderita strok iskemik dengan lokasi lesi hipodens subkortikal yang mengalami gangguan kualitas tidur sebesar 82,1% dan lokasi lesi hipodens kortikal sebesar 33,3%

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Yin, Weihong, Christopher D. Porada, Stephen Walker, Colin Bishop i Graca Almeida-Porada. "RUNX1C-Mediated Reprogramming Of Human Bone Marrow Stromal Cells into Early Blood Progenitors". Blood 122, nr 21 (15.11.2013): 2463. http://dx.doi.org/10.1182/blood.v122.21.2463.2463.

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Abstract Somatic cell reprogramming to the hematopoietic lineage, either through a pluripotent state or directly, opens the possibility of production of a ready source of autologous hematopoietic stem cells (HSC) that can be used to treat/cure a wide variety of blood disorders. While it has previously been shown that dermal fibroblasts (HFF) can be directly reprogrammed to the hematopoietic lineage, the efficiency was relatively low and the resultant hematopoietic cells lacked multilineage differentiative potential. Stro1(+) isolated stromal progenitors (SIPs) can easily be isolated from the bone marrow (BM) and expanded ex-vivo to obtain clinically significant numbers of cells. In similarity to HSC, SIPs are derived from the mesoderm, and are intimately linked with HSC specification during ontogeny. As such, they are likely to be epigenetically closer to HSC than HFF, and therefore good candidates for reprogramming into hematopoietic cells. To verify the uniqueness of SIPs for reprogramming, we transduced SIPs and HFF with OCT4 and/or RUNX1C, a master transcription factor (TF) that triggers the developmental onset of definitive hematopoiesis, in the following combinations: 1) OCT4 alone; 2) RUNX1C alone; or 3) OCT4+RUNX1C. We then performed a timeline of gene/cell surface marker expression (using microarray, qRT-PCR, and flow cytometry) from day 3-16 post-transduction. Visual inspection of the cultures showed that, while reprogrammed colonies began to appear in SIPs cultures at day 9, no colonies were seen during this time period in HFF cultures. Flow cytometry and molecular analyses of colonies obtained from OCT4+RUNX1C combination demonstrated that expression of CD41, the earliest marker of commitment to the hematopoietic lineage, commenced within only 3-4 days and peaked at day 5-6, by which time ∼20% of SIPs expressed this marker. This peak in CD41 expression coincided with commencement of expression of CD34 and CD45, and maximal induction of several hematopoiesis-specific TFs and phenotypic markers such as PU.1, HOXB4, GATA2, MIXL, WNT3, KDR, CDX4, which occurred at 1-3 logs higher levels in SIPs than HFF. Further studies demonstrated that the chromatin remodeling function of OCT4 could be replaced with the histone methyltransferase inhibitor Bix-01294, with the combination of RUNX1C and Bix-01294 inducing levels of CD34 and CD41 expression by day 5 that were similar to those achieved with RUNX1C plus OCT4. The present studies thus take several important steps towards making the promise of producing autologous hematopoietic cells for transplantation via direct reprogramming a reality. Disclosures: No relevant conflicts of interest to declare.

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Rogoziński, Piotr. "Obowiązek zawiadamiania stron o kontynuacji rozprawy w aspekcie gwarancji procesowych oraz zasady koncentracji procesu". Ruch Prawniczy, Ekonomiczny i Socjologiczny 75, nr 2 (2.11.2018): 99–111. http://dx.doi.org/10.14746/rpeis.2013.75.2.8.

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Abstrakt: W artykule zwrócono uwagę na kwestię obowiązku zawiadamiania przez sąd stron o kontynuacji rozprawy w świetle tego, że zapewnienie rzeczywistej możliwości udziału stron w rozprawie głównej świadczy o respektowaniu przez dany system prawny najważniejszych zasad i gwarancji procesowych. Obowiązek zawiadamiania stron o kontynuacji rozprawy należy rozpatrywać w świetle gwarancji procesowych stron oraz zasady koncentracji procesu, gdyż pojęcia te odzwierciedlają kierunki najważniejszych interesów, jakie ścierają się w procesie, a mianowicie: interesów indywidualnych (przede wszystkim stron) oraz interesu społecznego. Interesy te są przy tym często rozbieżne. Kwestia zawiadamiania stron o kontynuacji rozprawy aktualizuje się w sytuacji, gdy zachodzi przerwanie ciągłości rozprawy. Na gruncie obowiązującego prawa ma to miejsce w związku z następującymi instytucjami procesowymi: zawieszeniem postępowania, odroczeniem rozprawy, przerwą w rozprawie, odroczeniem wydania wyroku i wznowieniem przewodu sądowego. Wątpliwości pojawiają się zwłaszcza wtedy, gdy przerwanie ciągłości rozprawy następuje z jednoczesnym wskazaniem czasu i miejsca jej kontynuowania, a nie wszystkie strony są obecne w chwili ogłaszania decyzji. W artykule podjęto próbę zanalizowania niektórych istotnych problemów nasuwających się w związku z zawiadamianiem stron o kontynuacji rozprawy na tle poszczególnych wyżej wskazanych instytucji procesowych.

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Rachmania, Nindita, Nia Kurnia Sholihat i Esti Dyah Utami. "Hubungan Karakteristik Pasien dengan Kepatuhan Minum Obat dan Kualitas Hidup Pasien Rawat Jalan Strok Iskemik di RSUD Banyumas". Acta Pharmaciae Indonesia : Acta Pharm Indo 8, nr 1 (31.03.2020): 16. http://dx.doi.org/10.20884/1.api.2020.8.1.2359.

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Pasien strok memiliki risiko tinggi pada terjadinya strok berulang sehingga pasien strok perlu diberikan terapi pencegahan yaitu obat antiplatelet. Kualitas hidup pada pasien pasca strok dapat membantu mengevaluasi terapi yang telah diterapkan. Tujuan penelitian ini adalah untuk mengetahui karakteristik pasien apa saja yang berhubungan terhadap kepatuhan minum obat dan kualitas hidup pasien strok iskemik di RSUD Banyumas. Jumlah sampel penelitian sebanyak 44 orang dan menggunakan teknik total sampling. Kepatuhan minum obat diukur menggunakan kuesioner MARS-5 sedangkan kuesioner WHOQOL-Bref digunakan untuk mengukur kualitas hidup. Hasil yang didapat pada penelitian ini adalah tidak ada hubungan antara karakteristik pasien yaitu jenis kelamin, usia, pendidikan, pekerjaan dan status tinggal terhadap kepatuhan minum obat antiplatelet. Terdapat hubungan yang signifikan antara karakteristik pasien yaitu usia (p=0.004), pendidikan (p=0.000), pekerjaan (p=0.013) dan status tinggal (p=0.042) terhadap kualitas hidup pasien strok iskemik.

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Bensidhoum, Morad, Alain Chapel, Sabine Francois, Christelle Demarquay, Christelle Mazurier, Loic Fouillard, Sandrine Bouchet i in. "Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment". Blood 103, nr 9 (1.05.2004): 3313–19. http://dx.doi.org/10.1182/blood-2003-04-1121.

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Abstract The Stro-1 antigen potentially defines a mesenchymal stem cell (MSC) progenitor subset. We here report on the role of human ex vivo-expanded selected Stro-1+ or Stro-1- MSC subsets on the engraftment of human CD34+ cord blood cells in the nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mouse model. The data show that cotransplantation of expanded Stro-1- cells with CD34+ cells resulted in a significant increase of human CD45, CD34, CD19, and CD11b cells detected in blood or in bone marrow (BM) and spleen as compared with the infusion of CD34+ cells alone. Infusion into mice of expanded Stro-1+ and Stro-1- cells (without CD34+ cells) showed that the numbers of Stro-1+-derived (as assessed by DNA analysis of human β-globin with quantitative polymerase chain reaction [PCR]) were higher than Stro-1--derived cells in spleen, muscles, BM, and kidneys, while more Stro-1--derived than Stro-1+-derived cells were found in lungs. The transduction of expanded Stro-1+ cells with an enhanced green fluorescent protein (eGFP) gene did not modify their cytokine release and their homing in NOD/SCID mouse tissues. The difference between the hematopoietic support and the homing capabilities of expanded Stro-1+ and Stro-1- cells may be of importance for clinical therapeutic applications: Stro-1+ cells may rather be used for gene delivery in tissues while Stro-1- cells may rather be used to support hematopoietic engraftment. (Blood. 2004;103:3313-3319)

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Yang, Xue Chun, i Zheng Hao Liu. "Running Speed Analysis of Car on Dual Strop Ropeway with Headwind Load". Advanced Materials Research 562-564 (sierpień 2012): 1522–25. http://dx.doi.org/10.4028/www.scientific.net/amr.562-564.1522.

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This paper indicates the influence on the operation of the strop ropeway under the action of headwind, and establishes a curve equation of strop ropeway with wind load in accordance with static balance theory. It makes a dynamic analysis on bi-cable tackle under the action of headwind load, and determines the acceleration of the tackle under wind load. Meanwhile, it analyzes the whole course of the bi-cable strop ropeway, the influence of the wind load on the running speed and result of the strop tackle under the headwind. The paper summarizes the calculation process for the speed of bi-cable strop tackles under different conditions and finally tested and verified it through illustration.

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Unseld, Robert. "Schlüsselfaktor Strom". ATZelektronik 14, nr 10 (październik 2019): 14–15. http://dx.doi.org/10.1007/s35658-019-0115-4.

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Larena-Avellaneda, Axel. "Unter Strom". Heilberufe 60, nr 7 (lipiec 2008): 24–26. http://dx.doi.org/10.1007/s00058-008-0110-2.

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Dewi, I. Gusti Ayu Agung Silvia Wulan, i David Hizkia Tobing. "Kualitas hidup pascastrok peserta yoga pada Komunitas Ambarashram, Ubud, Bali". Jurnal Psikologi Udayana 6, nr 02 (31.10.2019): 347. http://dx.doi.org/10.24843/jpu.2019.v06.i02.p14.

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Strok merupakan faktor penyebab kematian kedua di dunia yang menyerang fungsi otak dan mengakibatkan kelumpuhan pada separuh bagian tubuh yang disebabkan oleh tekanan darah tinggi, penyakit jantung, stres, faktor kelelahan dan kurang berolahraga. Strok merupakan penyebab utama kualitas hidup yang buruk karena adanya gejala sisa yang memengaruhi keseluruhan aspek kehidupan. Kualitas hidup memengaruhi kondisi pada penderita strok seperti kondisi fisik, psikologis dan hubungan sosial. Upaya untuk mengurangi dampak strok dapat dilakukan dengan melibatkan seluruh anggota tubuh untuk bergerak secara rutin, serta dengan melakukan manajemen perawatan diri. Salah satu upaya yang dapat dilakukan adalah yoga. Yoga menjadi pengobatan bidang kedokteran serta perawatan psikologis. Yoga merupakan teknik pelatihan pengontrol kecemasan serta keharmonisan antara pikiran dan tubuh yang memiliki manfaat mengurangi keluhan fisik dan mengendalikan kestabilan emosi. Tujuan dari penelitian ini untuk mengetahui bagaimana kualitas hidup peserta yoga pascastrok yang mengikuti yoga pada sebuah komunitas Ambarashram yang terletak di Ubud, Bali. Metode yang digunakan dalam penelitian ini adalah metode kualitatif dengan pendekatan fenomenologi. Pada peneltian ini terdapat lima responden dengan rentang usia 40 – 55 tahun yang memiliki riwayat strok dan mengikuti yoga. Data dikumpulkan dengan teknik wawancara mendalam dan observasi. Hasil dari penelitian ini menunjukkan bahwa kondisi individu pascastrok digambarkan melalui empat tema yaitu penyebab strok, dampak strok, manfaat yoga dan gejala jika tidak rutin melakukan yoga. Responden dalam penelitian ini mengalami perubahan kualitas hidup ke arah positif setelah mengikuti yoga secara rutin. Kata kunci: Kualitas hidup, strok, yoga.

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Thierry 9, Dominique, Y. Z. Zhang 1, A. Chapel 2, M. Benshidoum 3, C. Mazurier 4, S. Bouchet 5, A. Nashef 6, M. C. Lopez 7, N. C. Gorin 8 i L. Fouillard 9. "Stro-1 Positive and Stro-1− Negtive Human Mesenchymal Stem Cells Express Different Levels of Immunosuppression." Blood 104, nr 11 (16.11.2004): 4964. http://dx.doi.org/10.1182/blood.v104.11.4964.4964.

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Abstract Mesenchymal stem cells (MSCs), have been shown to elicit immunosuppressive effect on allogeneic lymphocyte response. However, MSCs are heterogeneous and data on the inhibitory abilities of different MSC subsets are lacking. In the present study, we selected Stro-1+ cells from human bone marrow and evaluated the inhibitory capability of this MSC subset in mixed lymphocyte reactions (MLRs) or in mitogen stimulation asssays, in comparison to that of Stro-1− cells. To evaluate the two MSC subsets for immunomodulation in vitro, we added 1,000–30,000 Stro-1+ or Stro-1− cells to MLR at the beginning of the experiment. When comparing the inhibitory effects of the two subsets, PBLs proliferation was significantly more inhibited by Stro-1+MSCs (11.0%–63.7%) than by stro-1−MSCs (35.5%-106%) (P<0.01). Furthermore, as few as 1,000 Stro-1+ MSC could inhibit lymphocyte proliferation more effectively than 10 times more (10,000 cells) Stro-1−cells. As it was observed with the mixed lymphocyte reaction, suppression of the response to the mitogen also occurred in a dose dependent fashion, but to a lesser extent with the Stro-1−cells (25.5%–80.1% vs 7.5%–38.4% in Stro-1+cells) (P<0.05). To investigate whether the difference of suppressive effect that we observed between Stro-1+ and Stro-1− cells, still exist when MSC subsets are separated physically from PBL, we performed MLR in the upper chamber of a transwell and we seeded the lower chamber either with Stro-1+ or Stro-1− cells. The inhibitory effect of Stro-1+ cells was significantly more profound than the one observed when Stro-1− cells were used in the Transwell culture system (p<0.05) (Figure 3), demonstrating that one or several soluble factors was involved in production of different suppressive effects. Cytokine and chemokine genes, IL-10, TGF-β1, SDF-1, SCF and IL-6 expression were evaluated in both MSC subsets by quantitative RT-PCR. Low levels of IL-6, SCF, SDF-1 were observed in Stro-1+, which induced a fold increase around 1 (0,96 ± 0,32; 0,96 ± 0,24; 0,96 ± 0,24), indicating that there is no signifiant difference of these genes expression between the two MSC subsets. However, we observed in Stro-1+ a decreased gene expression for IL-10 (0,24 fold ± 0,59; p <0,05) and for TGF b1 (0,43 fold ± 0,32; p <0,05). This finding suggested that the candidate T-cell inhibitory factors TGF b1, IL-10, which are lower expressed in Stro-1+ cells, are not responsible for the more profound inhibition of immunoreactivity by Stro-1+ cells. We show here that significant differences do exist within these two subsets. Stro-1+ cells inhibit lymphocyte proliferation significantly more profoundly than Stro-1−cells. The difference is in part mediated by soluble factors, but not IL-10 and TGF-β1. These results point to the notion that Stro-1+ cells can elicit more powerful immunosuppressive ability and a pre-selection of Stro-1+MSC for clinical use may be advisable. These findings suggest that pre-selection of MSC before clinical use might produce more effective immunosuppression in different therapeutic applications, especially in clinics for the prevention of graft versus host disease (GVHD).

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Wieczorek, Mariusz. "Duty of Loyalty of the Employment Relationship Parties". Annales Universitatis Mariae Curie-Skłodowska, sectio G, (Ius) 65, nr 2 (30.12.2018): 301–14. http://dx.doi.org/10.17951/g.2018.65.2.301-314.

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Putri, Tissi Liskawini, Ratna Akbari Ganie i Aldy S. Rambe. "NEUTROPHIL-LYMPHOCYTE RATIO AND HIGH SENSITIVITY C-REACTIVE PROTEIN AS ISCHEMIC STROKE OUTCOME PREDICTOR (Rasio Neutrofil–Limfosit dan High Sensitivity C–Reactive Protein sebagai Peramal Hasilan Strok Iskemik Akut)". INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 23, nr 3 (14.04.2018): 240. http://dx.doi.org/10.24293/ijcpml.v23i3.1201.

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Proses inflamasi merupakan perjalanan penyakit dari strok iskemik akut, yang melibatkan penumpukan mediator inflamasi daninfiltrasi leukosit. Nilai Rasio Neutrofil-Limfosit (RNL) di beberapa penelitian dapat digunakan untuk meramalkan strok akibat iskemikakut yang caranya mudah dilakukan. High sensitivity C Reactive Protein (hs-CRP) merupakan reaktan tahap akut yang kadarnyameningkat di strok iskemik. Oleh karena itu bermanfaat sebagai petanda peramal hal terkait. Penelitian ini bertujuan untuk mengetahuiperbedaan nilai antara RNL dan hs-CRP dalam meramalkan hasilan pasien strok iskemik akut. Metode penelitian analitik observasionaldengan rancangan kohort prospektif. Hasil dinilai dengan modified Rankin Scale (mRS) (1–2=baik; 3–6=buruk) dan Barthel Index (BI)(0–20=ketergantungan jumlah keseluruhan, 21-60=berat; 1–90=sedang; 91–99=ringan dan 100=normal). Dari 43 sampel, didapatkanlaki-laki 24 orang (55,8%) dan perempuan 19 orang (44,2%) dengan rerata usia 57,12 ± 9,8 tahun. hubungan positif didapatkansedang dan bermakna antara RNL dengan hasilan mRS dan BI pasien strok iskemik akut (r=0,585; p=0,001 dan r=0,564; p=0,001).Hubungan positif didapatkan kuat dan bermakna antara hs-CRP dan hasilan mRS (r=0,614; p=0,001) serta didapatkan hubunganpositif dengan kekuatan sangat kuat dan bermakna antara hs-CRP dan hasilan n BI pasien strok iskemik akut (r=0,881; p=0,001).Dengan membandingkan ketepatan kedua data didapatkan RNL 86% dan hs-CRP 88% (p=0,6554). Perbedaan tidak bermakna terdapatantara nilai RNL dan hs-CRP sebagai peramal hasilan pasien strok iskemik akut.

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Brändlein, Philip. "Essity stellt Zewa teils auf Stroh um". Lebensmittel Zeitung 73, nr 23 (2021): 14. http://dx.doi.org/10.51202/0947-7527-2021-23-014-2.

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Wang, Xiaoxiao, Yanlan Wang, Xubin Dai, Tianyu Chen, Fanqiao Yang, Shuangye Dai, Qianmin Ou, Yan Wang i Xuefeng Lin. "Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells". Stem Cells International 2016 (2016): 1–9. http://dx.doi.org/10.1155/2016/4027542.

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Periodontitis is the most common cause of tooth loss and bone destruction in adults worldwide. Human periodontal ligament stem cells (hPDLSCs) may represent promising new therapeutic biomaterials for tissue engineering applications. Stromal precursor antigen-1 (STRO-1) has been shown to have roles in adherence, proliferation, and multipotency. Parathyroid hormone (PTH) has been shown to enhance proliferation in osteoblasts. Therefore, in this study, we aimed to compare the functions of STRO-1(+) and STRO-1(−) hPDLSCs and to investigate the effects of PTH on the osteogenic capacity of STRO-1(+) hPDLSCs in order to evaluate their potential applications in the treatment of periodontitis. Our data showed that STRO-1(+) hPDLSCs expressed higher levels of the PTH-1 receptor (PTH1R) than STRO-1(−) hPDLSCs. In addition, intermittent PTH treatment enhanced the expression of PTH1R and osteogenesis-related genes in STRO-1(+) hPDLSCs. PTH-treated cells also exhibited increased alkaline phosphatase activity and mineralization ability. Therefore, STRO-1(+) hPDLSCs represented a more promising cell resource for biomaterials and tissue engineering applications. Intermittent PTH treatment improved the capacity for STRO-1(+) hPDLSCs to repair damaged tissue and ameliorate the symptoms of periodontitis.

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Wrońska, Marta, i Małgorzata Plechawska-Wójcik. "Analiza porównawcza biblioteki jQuery Mobile i frameworka Bootstrap w wytwarzaniu stron responsywnych". Journal of Computer Sciences Institute 2 (30.12.2016): 89–92. http://dx.doi.org/10.35784/jcsi.119.

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Artykuł przedstawia analizę porównawczą technologii jQuery Mobile oraz frameworka Bootstrap w procesie wytwarzania stron responsywnych. Porównanie odbywa się na podstawie dwóch stron wykonanych w tych obu technologiach. Kryteriami analizy są: kompatybilność z urządzeniami mobilnymi, ułatwienia i gotowe komponenty, szybkość ładowania na różnych urządzeniach, wielkość kodu oraz zgodność ze standardami W3C i Google. Każda z technologii w dużym stopniu ułatwia proces tworzenia stron, aczkolwiek jQuery Mobile jest narzędziem znacznie bardziej rozbudowanym.

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Witek-Hajduk, Marzanna K., i Elżbieta Wąsowicz-Zaborek. "Społeczno-demograficzne uwarunkowania lokalizacji stron internetowych przedsiębiorstw". Studia i Prace Kolegium Zarządzania i Finansów, nr 169 (2.08.2019): 131–47. http://dx.doi.org/10.33119/sip.2018.169.9.

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Jednym z podstawowych dylematów związanych z marketingiem międzynarodowym jest standaryzacja vs lokalizacja działań marketingowych na rynku międzynarodowym. Problem ten dotyczy też stron internetowych przedsiębiorstw i ich marek będących kluczowym instrumentem marketingu internetowego. Wciąż jednak jest niewiele badań na temat czynników warunkujących lokalizację stron internetowych innych niż różnice kultur narodowych.Celem artykułu jest więc identyfikacja czynników społeczno-demograficznych w otoczeniu przedsiębiorstwa na rynkach zagranicznych, które mogą warunkować lokalizację stron WWW adresowanych do zagranicznych użytkowników. W artykule zastosowano metodę integracyjnego przeglądu literatury i zidentyfikowano następujące czynniki społeczno-demograficzne w kontekście lokalizacji stron WWW: wielkość populacji użytkowników Internetu, stopień penetracji Internetu w kraju/regionie, różnice struktury internautów ze względu na wiek/generację, płeć oraz przynależność do grupy społecznej.

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Halil, Fasni, Hj Darmawaty ER i Ruland DN Pakasi. "KADAR ALBUMIN SERUM PENDERITA STROK ISKEMIK DAN STROK HEMORAGIK". INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 16, nr 2 (16.03.2018): 55. http://dx.doi.org/10.24293/ijcpml.v16i2.956.

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To differentiate the ischemic and hemorrhagic stroke with the most accurate method can be carried out by Computerized Tomography(CT) scan. However, because the restrictor on access and cost, not all patients could gain the advantage of CT scan. Examination ofalbumin is a quick and easy test. The aim of this study was to evaluate the serum albumin level in patient suffering ischemic andhemorrhagic stroke. A cross sectional study of 60 ischemic and hemorrhagic stroke patients was performed at the Neurologic Departmentof Dr. Wahidin Sudirohusodo Hospital Makassar, from March up to August 2008. Albumin – levels was measured using the colorimetricBCG method, using the Cobas Integra 400 Autoanalyser. The data were than analyzed with SPSS 11.5 software and t test. Among the60 samples of the ischemic and hemorrhagic stroke were found mean values of serum albumin level is 3.38 ± 0.120 and hemorrhagicstroke is 3.51 ± 0.0938 with p value is 0.495. There were no significantly different between the serum albumin level of the ischemicand hemorrhagic stroke patients.

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Simmons, PJ, i B. Torok-Storb. "Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1". Blood 78, nr 1 (1.07.1991): 55–62. http://dx.doi.org/10.1182/blood.v78.1.55.55.

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Abstract Murine IgM monoclonal antibody STRO-1 identifies a cell surface antigen expressed by stromal elements in human bone marrow (BM). STRO-1 binds to approximately 10% of BM mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage [CFU-GM], erythroid bursts [BFU-E], and mixed colonies [CFU-Mix]). Fibroblast colony-forming cells (CFU-F) are present exclusively in the STRO-1+ population. Dual-color cell sorting using STRO-1 in combination with antibody to glycophorin A yields a population approximately 100-fold enriched in CFU-F in the STRO-1+/glycophorin A+ population. When plated under long-term BM culture (LTBMC) conditions, STRO-1+ cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. STRO-1+ cells isolated from LTBMC at later times retain the capacity to generate adherent layers with a cellular composition identical to that of the parent cultures. The STRO-1-selected adherent layers are able to support the generation of clonogenic cells and mature hematopoietic cells from a population of CD34+ cells highly enriched in so-called long-term culture-initiating cells. We conclude that antibody STRO-1 binds to BM stromal elements with the capacity to transfer the hematopoietic microenvironment in vitro.

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Simmons, PJ, i B. Torok-Storb. "Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1". Blood 78, nr 1 (1.07.1991): 55–62. http://dx.doi.org/10.1182/blood.v78.1.55.bloodjournal78155.

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Murine IgM monoclonal antibody STRO-1 identifies a cell surface antigen expressed by stromal elements in human bone marrow (BM). STRO-1 binds to approximately 10% of BM mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage [CFU-GM], erythroid bursts [BFU-E], and mixed colonies [CFU-Mix]). Fibroblast colony-forming cells (CFU-F) are present exclusively in the STRO-1+ population. Dual-color cell sorting using STRO-1 in combination with antibody to glycophorin A yields a population approximately 100-fold enriched in CFU-F in the STRO-1+/glycophorin A+ population. When plated under long-term BM culture (LTBMC) conditions, STRO-1+ cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, and fibroblastic elements. STRO-1+ cells isolated from LTBMC at later times retain the capacity to generate adherent layers with a cellular composition identical to that of the parent cultures. The STRO-1-selected adherent layers are able to support the generation of clonogenic cells and mature hematopoietic cells from a population of CD34+ cells highly enriched in so-called long-term culture-initiating cells. We conclude that antibody STRO-1 binds to BM stromal elements with the capacity to transfer the hematopoietic microenvironment in vitro.

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Johan, Mhd, i Alpino Susanto. "Gangguan Bertutur pada Penderita Strok: Suatu Kajian Neurolinguistik". Deiksis : Jurnal Pendidikan Bahasa dan Sastra Indonesia 5, nr 2 (31.07.2018): 112. http://dx.doi.org/10.33603/deiksis.v5i2.1127.

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Penelitian ini merupakan kajian neurolinguistik yang bertujuan untuk menjelaskanbentukgangguan fonem yang dilafalkan oleh penderita strok. Teknik pengumpulan data yang digunakan yakni dengan teknik rekam dan metode Simak Libat Cakap (SLC). SelanjutnyaÂ untuk menganalisis data, penulisÂ menggunakan metode agih dan teknik bagi unsur langsung. Teknik bagi unsur langsung akan didukung teknik-teknik dasar dan teknik lanjutan seperti teknik lesap, teknik ganti, teknik ulang, dan teknik ekspansi. Adapun hasil penelitian membuktikan bahwa gangguan yang terjadi pada penderita strok pada saat melafalkan fonem yakni adanya penghilangan, penambahan dan pergantian fonemÂ pada posisi yang tidak menentu. Penderita strok yang mengalami gangguan pada saraf kiri tidak dapat melafalkan fonem-fonem dengan tepat. Gangguan ini termasuk kategori afasia dan disartria. Adapun hasil penelitian menunjukkan bahwa peristiwa yang terjadi pada para penderita strok adalah peristiwa pelesapan, perganti, dan penambahan fonem.

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Ajay P, Lamba. "Aaitihasik Granthoma Strot Sahitya - Ek Adhyayan". Paripex - Indian Journal Of Research 2, nr 1 (15.01.2012): 5. http://dx.doi.org/10.15373/22501991/jan2013/30.

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Szewczyk, Agnieszka. "The quality of internet pages relating to computer games". Zeszyty Naukowe Uniwersytetu Szczecińskiego. Studia Informatica 39 (2016): 121–35. http://dx.doi.org/10.18276/si.2016.39-11.

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Brandt, A. "Thermisches Überwachungssystem „Hotspot„". Technische Sicherheit 10, nr 03 (2020): 24–25. http://dx.doi.org/10.37544/2191-0073-2020-03-24.

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Wir nehmen unseren Strom oft als selbstverständlich hin. Doch praktisch alles, was Strom als Versorgungsquelle nutzt, hat das Potenzial, Brände zu verursachen. In einem typischen Gebäude umfasst dies die feste Verkabelung sowie alle damit verbundenen Geräte und Einrichtungen.

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Pasche, Eckart. "„Strom können wir!“". VDI nachrichten 74, nr 48 (2020): 27. http://dx.doi.org/10.51202/0042-1758-2020-48-27.

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Rapp, Christoph, Florian Schwertfirm i Christian Hickisch. "Strom aus Beton?" WASSERWIRTSCHAFT 107, nr 10 (październik 2017): 75–76. http://dx.doi.org/10.1007/s35147-017-0162-9.

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Auer, Walter. "Energieeffizienz braucht Strom". e & i Elektrotechnik und Informationstechnik 126, nr 10 (październik 2009): 347. http://dx.doi.org/10.1007/s00502-009-0669-6.

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DÖpfer, Eckhard. "Bezahlbar unter Strom". H&V Journal 65, nr 3 (marzec 2013): 3. http://dx.doi.org/10.1365/s35824-013-0243-1.

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Ilse, Klemens. "Staub oder Strom?" UmweltMagazin 50, nr 12 (2020): 53–55. http://dx.doi.org/10.37544/0173-363x-2020-12-53.

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Viel Geld geht der Solarbranche jährlich verloren, weil Photovoltaik-Module verschmutzen. Forscher untersuchen, wie man diesem „Soiling“ entgegenwirken kann. Durch ein besseres Verständnis des Phänomens lassen sich Gegenmaßnahmen für Wüstengebieten entwickeln.

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Agler-Rosenbaum, Miriam, Uwe Schröder i Falk Harnisch. "Mikroben unter Strom". Biologie in unserer Zeit 43, nr 2 (kwiecień 2013): 96–103. http://dx.doi.org/10.1002/biuz.201310502.

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Marohn, Annette. "Ionenbildung durch Strom?" CHEMKON 15, nr 2 (kwiecień 2008): 75–84. http://dx.doi.org/10.1002/ckon.200810073.

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Rostalski, Walter. "Gewebe unter Strom". physiopraxis 3, nr 04 (kwiecień 2005): 32–34. http://dx.doi.org/10.1055/s-0032-1308358.

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Elektrotherapie unterstützt Physiotherapie – sinnvoll und effektiv. Sie kann Schmerzen lindern, die Ödemresorption fördern und auf das Vegetativum wirken. Thieme-Autor Walter Rostalski gibt einen Überblick über gängige Stromformen und deren praktische Anwendung am Patienten.

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Jahn, Claus. "Magen unter Strom?" Deutsche Heilpraktiker-Zeitschrift 13, nr 06 (wrzesień 2018): 42–46. http://dx.doi.org/10.1055/a-0645-6771.

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SummaryDie Funktionelle Dyspepsie wird als Ausschlussdiagnose gestellt, wenn für chronische Magenbeschwerden keine organische Ursache gefunden wird. Leitsymptome der Funktionellen Dyspepsie sind epigastrischer Schmerz, epigastrisches Brennen, Völlegefühl und vorzeitiges Sättigungsgefühl. Als naturheilkundliche Therapeutika kommen unter anderem entkrampfende, schmerzstillende und magensäureregulierende Injektabilia infrage.

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Bulmahn, Maren. "Biokraftstoff aus Stroh". Nachrichten aus der Chemie 66, nr 12 (grudzień 2018): 1167. http://dx.doi.org/10.1002/nadc.20184080740.

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Schützenmeister, Nina, i Maik Assmann. "Katalyse unter Strom". Nachrichten aus der Chemie 67, nr 7-8 (lipiec 2019): 67–70. http://dx.doi.org/10.1002/nadc.20194089018.

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Bauer, Martin. "Sauber unter Strom". JOT Journal für Oberflächentechnik 47, nr 11 (listopad 2007): 64–65. http://dx.doi.org/10.1007/bf03241766.

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Weber, Florian, i Olaf Kühne. "Räume unter Strom". Raumforschung und Raumordnung 74, nr 4 (31.08.2016): 323–38. http://dx.doi.org/10.1007/s13147-016-0417-4.

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Zusammenfassung Die Energiewende verändert in Deutschland mit dem Ausstieg aus der Kernkraft und dem Ausbau erneuerbarer Energien in weitreichender Weise bisherige Strukturen der Energieversorgung und wirkt sich dabei räumlich stark aus. Einen Aspekt bilden hierbei Veränderungen im bestehenden Stromnetz. Vorhandene Leitungstrassen sollen ertüchtigt, andere umfänglich neu gebaut werden, was Widerstände und Konflikte mit sich bringt. Der Artikel untersucht vor diesem Hintergrund aus diskurstheoretischer Perspektive, wie der Stromnetzausbau und mögliche Widerstände verhandelt werden und welche Argumentationsmuster dabei vorherrschend sind. Die durchgeführten Analysen fußen auf einem Methodenmix aus quantitativ orientierten und qualitativen Analysebestandteilen, um sowohl zentrale Bezugnahmen auszudifferenzieren als auch Einzelaspekte detaillierter zu betrachten. Zusammenfassend bilden die Bedarfsfrage des Stromnetzausbaus, Beteiligung, die eingesetzte Technik, Gesundheit, Wirtschaft sowie Natur und Landschaft zentrale Konfliktfelder, die innerhalb des Stromnetzausbaus ausgehandelt werden und sich in eher kognitive, emotionale und ästhetische Bewertungsmuster einreihen.

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