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Artykuły w czasopismach na temat „Techniques de knock-out de gènes”

Autor: Grafiati

Data publikacji: 21 września 2024

Data aktualizacji: 31 lipca 2025

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1

Wolf, Steven E., and Kenneth J. Woodside. "Transgenic and gene knock-out techniques and burn research." Journal of Surgical Research 123, no. 2 (2005): 328–39. http://dx.doi.org/10.1016/j.jss.2004.06.001.

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Monahan, Paul E. "Factor IX: Insights from knock-out and genetically engineered mice." Thrombosis and Haemostasis 100, no. 10 (2008): 563–75. http://dx.doi.org/10.1160/th08-04-0262.

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SummaryThe study of coagulation factors has been rapidly advanced by studies performed in genetically engineered mouse strains. Investigation of factor IX (FIX) has benefited from excellent genedeleted mouse models that recapitulate many of the features of human haemophilia B. Moreover, advanced positional cloning techniques and availability of technology to allow not only knock-out mice, but also knock-in and knock-down mice, provide new opportunities to observe genotype-phenotype and structure-function correlations regarding FIX, as well as the interaction of FIX with inflammatory, immune, a

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Ohara, Hiroki, and Toru Nabika. "Genetic Modifications to Alter Blood Pressure Level." Biomedicines 10, no. 8 (2022): 1855. http://dx.doi.org/10.3390/biomedicines10081855.

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Genetic manipulation is one of the indispensable techniques to examine gene functions both in vitro and in vivo. In particular, cardiovascular phenotypes such as blood pressure cannot be evaluated in vitro system, necessitating the creation of transgenic or gene-targeted knock-out and knock-in experimental animals to understand the pathophysiological roles of specific genes on the disease conditions. Although genome-wide association studies (GWAS) in various human populations have identified multiple genetic variations associated with increased risk for hypertension and/or its complications, t

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Yang, Caiting, Yu Lei, Tinglin Ren, and Mingze Yao. "The Current Situation and Development Prospect of Whole-Genome Screening." International Journal of Molecular Sciences 25, no. 1 (2024): 658. http://dx.doi.org/10.3390/ijms25010658.

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High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function genetic screening is mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and CRISPR interference (CRISPRi) technologies. Gain-of-function genetic screening mainly depends on the overexpression of a cDNA library and CRISPR activation (CRISPRa). Base editing can perform both gain- and loss-of-function genetic screening. This review discusses genetic screening techniques based on Cas9 nuclease, including Cas

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Syarifuddin, Syarifuddin, Ade Ulansari, and Iftahurrahmah Iftahurrahmah. "ARCHITECTURAL DESIGN AND WOODEN SRUCTURES OF TRADITIONAL OGAN ILIR BUILDINGS AS THE LOCAL CULTURAL WEALTH OF THE OGAN ILIR COMMUNITY." Sosiohumaniora 24, no. 2 (2022): 256. http://dx.doi.org/10.24198/sosiohumaniora.v24i2.36893.

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South Sumatra has a lot of traditional houses across its region. Each house has its own characteristics. One notable design is houses built on stilts. In Ogan Ilir, especially Tanjung Batu, it is famous for the production of knock-down houses. Knock-down houses in Tanjung Batu are a stilt house with an architectural design that almost resembles a Limas house, only that these knock-down houses are specially designed to make it easy to disassemble and reassemble them elsewhere. This study aims to give a closer look at the design, structure and wood choices of knock-down houses that have become u

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Riyandi, Ages, and Desy Misnawati. "STRATEGI PEMASARAN PRODUK RUMAH KNOCK DOWN PADA MASYARAKAT TANJUNG BATU SEBERANG." Jurnal Inovasi 15, no. 1 (2021): 11–27. http://dx.doi.org/10.33557/ji.v15i1.2200.

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The production of knock down houses is in a great demand especially in Tanjung BatuSeberang.The craft of knxok down houses was passed down from the era of Sriwijaya Kingdom untilnow. The demand of the craft is inscreasing from year to year this has turned out to be anopportunity so that many young people are engaged in this business. Kotler and Armstrong'sMarketing Strategy Theory (2004 in Varadarajan 2009). The research method uses qualitativeresearch. Collecting data through observation as the main source, interviews and documentation.While data analysis techniques, data presentation and dat

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Mittal, Vikram. "A Review of Recent Advancements in Knock Detection in Spark Ignition Engines." Signals 5, no. 1 (2024): 165–80. http://dx.doi.org/10.3390/signals5010009.

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In gasoline engines, the combustion process involves a flame’s propagation from the spark plug to the cylinder walls, resulting in the localized heating and pressurization of the cylinder content ahead of the flame, which can lead to the autoignition of the gasoline and air. The energy release from the autoignition event causes the engine cylinder to resonate, causing an unpleasant noise and eventual engine damage. This process is termed as knock. Avoiding knock has resulted in limiting the maximum engine pressures, and hence limiting the maximum efficiencies of the engine. Modern engines empl

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Fabbri, Mattia, and Pier Giuseppe Giribone. "Design, implementation and validation of advanced lattice techniques for pricing EAKO — European American Knock-Out option." International Journal of Financial Engineering 06, no. 04 (2019): 1950032. http://dx.doi.org/10.1142/s2424786319500324.

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The paper presents a series of advanced lattice methods aimed at evaluating an EAKO European-American Knock-Out contract. The first part of the paper deals with the numerical methods implemented for pricing: Binomial and Trinomial Stochastic trees, Adaptive Mesh Model, Pentanomial and Heptanomial lattice. In the second part, specific tests are designed to validate the code written in Matlab language. The study concludes by applying the most performing model to a real market case.

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Balla, Beata, Florin Tripon, and Claudia Banescu. "From Descriptive to Functional Genomics of Leukemias Focusing on Genome Engineering Techniques." International Journal of Molecular Sciences 22, no. 18 (2021): 10065. http://dx.doi.org/10.3390/ijms221810065.

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Genome engineering makes the precise manipulation of DNA sequences possible in a cell. Therefore, it is essential for understanding gene function. Meganucleases were the start of genome engineering, and it continued with the discovery of Zinc finger nucleases (ZFNs), followed by Transcription activator-like effector nucleases (TALENs). They can generate double-strand breaks at a desired target site in the genome, and therefore can be used to knock in mutations or knock out genes in the same way. Years later, genome engineering was transformed by the discovery of clustered regularly interspaced

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10

Li, Meng, Xufang Niu, Shuang Li, et al. "CRISPR/Cas9 Based Cell-Type Specific Gene Knock-Out in Arabidopsis Roots." Plants 12, no. 12 (2023): 2365. http://dx.doi.org/10.3390/plants12122365.

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CRISPR/Cas9 (hereafter Cas9)-mediated gene knockout is one of the most important tools for studying gene function. However, many genes in plants play distinct roles in different cell types. Engineering the currently used Cas9 system to achieve cell-type-specific knockout of functional genes is useful for addressing the cell-specific functions of genes. Here we employed the cell-specific promoters of the WUSCHEL RELATED HOMEOBOX 5 (WOX5), CYCLIND6;1 (CYCD6;1), and ENDODERMIS7 (EN7) genes to drive the Cas9 element, allowing tissue-specific targeting of the genes of interest. We designed the repo

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11

Fernández, Juan Pablo, Björn Petersen, Petra Hassel, et al. "Comparison Between Electroporation at Different Voltage Levels and Microinjection to Generate Porcine Embryos with Multiple Xenoantigen Knock-Outs." International Journal of Molecular Sciences 25, no. 22 (2024): 11894. http://dx.doi.org/10.3390/ijms252211894.

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In the context of xenotransplantation, the production of genetically modified pigs is essential. For several years, knock-out pigs were generated through somatic cell nuclear transfer employing donor cells with the desired genetic modifications, which resulted in a lengthy and cumbersome procedure. The CRISPR/Cas9 system enables direct targeting of specific genes in zygotes directly through microinjection or electroporation. However, these techniques require improvement to minimize mosaicism and low mutation rates without compromising embryo survival. This study aimed to determine the gene edi

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12

Taylor, R., A. F. Steinberg, J. Pasternak, R. B. Appleby, S. L. Sheehy, and E. Benedetto. "Slow Extraction Techniques from Fixed Field Accelerators." Journal of Physics: Conference Series 2687, no. 2 (2024): 022022. http://dx.doi.org/10.1088/1742-6596/2687/2/022022.

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Abstract Fixed Field Accelerators are a candidate for future hadron cancer therapy facilities as their high repetition rate and large energy acceptance enables novel treatment modalities such as high dose rate FLASH. However, conventional dose delivery mechanisms are still necessary, requiring continuous beam delivery over 1–30s. This work is the first study of slow extraction from a scaling Fixed Field Accelerator, using the LhARA facility for baseline parameters. At a horizontal tune of 10/3, the intrinsic sextupole strength of the nonlinear FFA magnetic field is sufficient to excite the res

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13

Solo, Katherine M., Sara B. Collins, Liesel G. Schneider, et al. "Evaluation of Floral Cuts on Eriophyid Mite Retention on Knock Out and Multiflora Rose Cuttings." Plant Health Progress 20, no. 2 (2019): 83–87. http://dx.doi.org/10.1094/php-12-18-0080-rs.

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Current eriophyid mite quantification techniques require transportation of the Rosa spp. cuttings to the laboratory. It is thought that the change in xylem hydraulic conductance within the cut cane could trigger the mites to abandon their host, owing to the changes to the microenvironments that these mites are inhabiting. An experiment was conducted to determine the necessity of floral cuts (reducing stem embolisms by an additional cut underwater) for the retention of eriophyid mites during transit. Four groups of plants (rose rosette virus (RRV)-free Knock Out roses, RRV-infected Knock Out ro

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14

Waheed, Usama, and Muhammad Naveed. "Enhancing Wheat Crop Yield and Quality through Genetic Engineering Technologies: A Comprehensive Review." Journal of Global Innovations in Agricultural Sciences 11, no. 4 (2023): 481–89. http://dx.doi.org/10.22194/jgias/11.1089.

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The increasing world population has created an alarming situation to meet the demand for food. Ultimately wheat yield is required to be increased proportionally to the world’s population. An estimated increase of 05 tonnes per hectare is direly needed from the current production of 3.3 tonnes by the end of 2050. To achieve this goal it is required to adopt new, emerging, and efficient breeding techniques which caused a recent breakthrough in wheat genome sequencing and provided sufficient information for the traits which are directly related to quality and yield of crops. This review provides

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Ostrowski, Martin, Sasha Tetu, Karl Hassan, et al. "From omics to systems biology: Exploring the mystery box of microbial life." Microbiology Australia 32, no. 4 (2011): 147. http://dx.doi.org/10.1071/ma11147.

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Microbial molecular biology has traditionally used very reductionist approaches; for example, find a gene of interest, clone it or knock it out and see if you can detect a phenotype. The genomics era has opened up the possibility of analysing microbes and communities at a systems level by combining high-throughput experimental data from genomic, transcriptomic, proteomic and phenomic techniques. This parallels earlier reductionist approaches by going from DNA to RNA to protein to phenotype, albeit on a global rather than individual gene scale.

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16

Allen, Daniel, Nechama Kalter, Michael Rosenberg, and Ayal Hendel. "Homology-Directed-Repair-Based Genome Editing in HSPCs for the Treatment of Inborn Errors of Immunity and Blood Disorders." Pharmaceutics 15, no. 5 (2023): 1329. http://dx.doi.org/10.3390/pharmaceutics15051329.

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Genome engineering via targeted nucleases, specifically CRISPR-Cas9, has revolutionized the field of gene therapy research, providing a potential treatment for diseases of the blood and immune system. While numerous genome editing techniques have been used, CRISPR-Cas9 homology-directed repair (HDR)-mediated editing represents a promising method for the site-specific insertion of large transgenes for gene knock-in or gene correction. Alternative methods, such as lentiviral/gammaretroviral gene addition, gene knock-out via non-homologous end joining (NHEJ)-mediated editing, and base or prime ed

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17

Russo, Emma E., Lola E. Zovko, Reza Nazari, Hendrik Steenland, Amy J. Ramsey, and Ali Salahpour. "Evaluation and Validation of Commercially Available Dopamine Transporter Antibodies." eneuro 10, no. 5 (2023): ENEURO.0341–22.2023. http://dx.doi.org/10.1523/eneuro.0341-22.2023.

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AbstractWith a wide variety of dopamine transporter (DAT) antibodies available commercially, it is important to validate which antibodies provide sufficient immunodetection for reproducibility purpose and for accurate analysis of DAT levels and/or location. Commercially available DAT antibodies that are commonly used were tested in western blotting (WB) on wild-type (WT) and DAT-knock-out (DAT-KO) brain tissue and with immunohistology (IH) techniques against coronal slices of unilaterally lesioned 6-OHDA rats, in addition to wild-type and DAT-knock-out mice. DAT-KO mice and unilateral 6-OHDA l

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18

DUCOS, A., B. BED'HOM, H. ACLOQUE, and B. PAIN. "Modifications ciblées des génomes : apports et impacts pour les espèces d’élevage." INRA Productions Animales 30, no. 1 (2018): 3–18. http://dx.doi.org/10.20870/productions-animales.2017.30.1.2226.

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L’avènement des nucléases programmables, et de CRISPR-Cas9 en particulier, constitue une réelle rupture technologique dans le domaine de l’ingénierie génétique. Le principe de ces méthodes est relativement simple. Il consiste en premier lieu à générer des cassures double-brin au niveau de régions très précises d’une séquence cible (ADN d’une cellule somatique, germinale, embryonnaire, iPS...). Ces cassures sont ensuite réparées par des mécanismes cellulaires pouvant conduire à l’inactivation des régions modifiées (knock-out, ou KO), ou à l’insertion, par recombinaison homologue, d’un fragment

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Salman, Ahmed, Samuel B. Hutton, Tutte Newall, et al. "Characterization of the Frmd7 Knock-Out Mice Generated by the EUCOMM/COMP Repository as a Model for Idiopathic Infantile Nystagmus (IIN)." Genes 11, no. 10 (2020): 1157. http://dx.doi.org/10.3390/genes11101157.

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In this study, we seek to exclude other pathophysiological mechanisms by which Frmd7 knock-down may cause Idiopathic Infantile Nystagmus (IIN) using the Frmd7.tm1a and Frmd7.tm1b murine models. We used a combination of genetic, histological and visual function techniques to characterize the role of Frmd7 gene in IIN using a novel murine model for the disease. We demonstrate that the Frmd7.tm1b allele represents a more robust model of Frmd7 knock-out at the mRNA level. The expression of Frmd7 was investigated using both antibody staining and X-gal staining confirming previous reports that Frmd7

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20

Murwaningtyas, Chatarina Enny, William Saputra Haryono, Maria Andriani Uge, and Tedi Kristofel. "Perbandingan Metode Monte Carlo Antithetic Variate dan Control Variate dalam Penentuan Harga Opsi Barrier Knock-Out." Euler : Jurnal Ilmiah Matematika, Sains dan Teknologi 12, no. 1 (2024): 37–44. http://dx.doi.org/10.37905/euler.v12i1.25128.

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This study aims to examine the effectiveness of the Monte Carlo antithetic variate and control variate methods in pricing knock-out barrier options compared to the standard Monte Carlo method. The main problem in barrier option pricing is the high variance of estimates, which can reduce the accuracy and efficiency of results. The standard Monte Carlo method often requires a very large number of simulations to achieve stable results, which is computationally inefficient. To address this issue, this study employs variance reduction techniques, antithetic variate, and control variate. The finding

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Taylor, R., E. Benedetto, M. Sapinski, and J. Pasternak. "Slow extraction modelling for NIMMS hadron therapy synchrotrons." Journal of Physics: Conference Series 2420, no. 1 (2023): 012101. http://dx.doi.org/10.1088/1742-6596/2420/1/012101.

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Abstract The Next Ion Medical Machine Study (NIMMS) is an umbrella R&D programme for CERN accelerator technologies targeting advanced accelerator options for proton and light ion therapy. In collaboration with the European programme HITRIplus, one area of study is slow extraction which is required to deliver a uniform beam spill for radiotherapy treatment. Several techniques use the third-order resonance to extract hadrons; these include betatron core driven extraction and radiofrequency knock-out. Flexible simulation tools using these techniques were prepared and initially benchmarked wit

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22

Hédou, Gaël, and Isabelle M. Mansuy. "Inducible molecular switches for the study of long-term potentiation." Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences 358, no. 1432 (2003): 797–804. http://dx.doi.org/10.1098/rstb.2002.1245.

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This article reviews technical and conceptual advances in unravelling the molecular bases of long-term potentiation (LTP), learning and memory using genetic approaches. We focus on studies aimed at testing a model suggesting that protein kinases and protein phosphatases balance each other to control synaptic strength and plasticity. We describe how gene ‘knock-out’ technology was initially exploited to disrupt the Ca 2+ /calmodulin-dependent protein kinase II α (CaMKII α ) gene and how refined knock-in techniques later allowed an analysis of the role of distinct phosphorylation sites in CaMKII

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23

AZAIEZ, F. "SHELL STRUCTURE EVOLUTION IN NUCLEI: NEW PARADIGM." International Journal of Modern Physics E 18, no. 10 (2009): 1986–91. http://dx.doi.org/10.1142/s0218301309014135.

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Shell structure evolution in nuclei situated at the extremes of neutron and proton excess are investigated using in-beam gamma spectroscopy techniques with radioactive beams at GANIL. A selection of results obtained very recently is presented: i) The reduced transition probabilities [Formula: see text] of the neutron-rich 74 Zn and 70 Ni nuclei have been measured using Coulomb excitation at intermediate energy. An unexpected large proton core polarization has been found in 70 Ni and interpreted as being due to the monopole interaction between the neutron g 9/2 and protons f 7/2 and f 5/2 spin-

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24

Jin, Binbin, Ding Zhao, Fei Liang, et al. "Electron-Beam Irradiation Induced Regulation of Surface Defects in Lead Halide Perovskite Thin Films." Research 2021 (June 4, 2021): 1–11. http://dx.doi.org/10.34133/2021/9797058.

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Organic-inorganic hybrid perovskites (OIHPs) have been intensively studied due to their fascinating optoelectronic performance. Electron microscopy and related characterization techniques are powerful to figure out their structure-property relationships at the nanoscale. However, electron beam irradiation usually causes damage to these beam-sensitive materials and thus deteriorates the associated devices. Taking a widely used CH3NH3PbI3 film as an example, here, we carry out a comprehensive study on how electron beam irradiation affects its properties. Interestingly, our results reveal that ph

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25

Lee, Gloria, Annie Lo, Sarah A. Short, et al. "Targeted gene deletion demonstrates that the cell adhesion molecule ICAM-4 is critical for erythroblastic island formation." Blood 108, no. 6 (2006): 2064–71. http://dx.doi.org/10.1182/blood-2006-03-006759.

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AbstractErythroid progenitors differentiate in erythroblastic islands, bone marrow niches composed of erythroblasts surrounding a central macrophage. Evidence suggests that within islands adhesive interactions regulate erythropoiesis and apoptosis. We are exploring whether erythroid intercellular adhesion molecule 4 (ICAM-4), an immunoglobulin superfamily member, participates in island formation. Earlier, we identified αV integrins as ICAM-4 counterreceptors. Because macrophages express αV, ICAM-4 potentially mediates island attachments. To test this, we generated ICAM-4 knock-out mice and dev

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26

Ghribi, Manel, Serge Basile Nouemssi, Fatma Meddeb-Mouelhi, and Isabel Desgagné-Penix. "Genome Editing by CRISPR-Cas: A Game Change in the Genetic Manipulation of Chlamydomonas." Life 10, no. 11 (2020): 295. http://dx.doi.org/10.3390/life10110295.

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Microalgae are promising photosynthetic unicellular eukaryotes among the most abundant on the planet and are considered as alternative sustainable resources for various industrial applications. Chlamydomonas is an emerging model for microalgae to be manipulated by multiple biotechnological tools in order to produce high-value bioproducts such as biofuels, bioactive peptides, pigments, nutraceuticals, and medicines. Specifically, Chlamydomonas reinhardtii has become a subject of different genetic-editing techniques adapted to modulate the production of microalgal metabolites. The main nuclear g

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Cary, Daniele C., and B. Matija Peterlin. "Targeting the latent reservoir to achieve functional HIV cure." F1000Research 5 (May 26, 2016): 1009. http://dx.doi.org/10.12688/f1000research.8109.1.

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While highly active anti-retroviral therapy has greatly improved the lives of HIV-infected individuals, current treatments are unable to completely eradicate the virus. This is due to the presence of HIV latently infected cells which harbor transcriptionally silent HIV. Latent HIV does not replicate or produce viral proteins, thereby preventing efficient targeting by anti-retroviral drugs. Strategies to target the HIV latent reservoir include viral reactivation, enhancing host defense mechanisms, keeping latent HIV silent, and using gene therapy techniques to knock out or reactivate latent HIV

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Regmi, Priti, and Tribikram Bhattarai. "Microbial Metabolic Engineering for Biopolymers Production." Journal of Nepal Biotechnology Association 6, no. 1 (2025): 55–65. https://doi.org/10.3126/jnba.v6i1.76920.

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This review delves into microbial engineering strategies for biopolymer production, which is crucial for tackling contemporary environmental challenges. It surveys microbial species capable of synthesizing biopolymers and examines genetic engineering techniques to enhance biopolymer yields. For the genetic manipulation approach, mainly the over-expression of the genes responsible for the production, the downregulation/knock out of competing branches, or the abundant supply of cofactors needed for the reaction are mostly tested, among others. This study provides insights into a few applications

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Lewis, Matthieu, Valerie Prouzet-Mauleon, Elodie Richard, Beatrice Turcq, Richard Iggo, and Francois-xavier Mahon. "Identification of Imatinib-Sensitizing Genes in Chronic Myeloid Leukemia with a Genome-Scale Crispr Knock-out Screen." Blood 128, no. 22 (2016): 4233. http://dx.doi.org/10.1182/blood.v128.22.4233.4233.

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Abstract Background: Resistance to tyrosine kinase inhibitors (TKIs) in chronic myeloid leukemia (CML) can either originate from mutations in the BCR-ABL1 gene, which are mostly well characterized, or emerge from unknown alternative mutations elsewhere in the genome. Small hairpin (sh)RNA screens have been used to discover such genes but are becoming limited due to sup-optimal protein depletion and non-reliable off-target effects. More efficient screening techniques in human cells are now available as a result of the increasing understanding of the CRISPR (Clustered Regularly Interspaced Short

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Indriani, Mutiya, and Nurhafizah Nurhafizah. "Pengembangan Kecerdasan Musikal Anak di Taman Kanak-Kanak Pertiwi 3 Kantor Gubernur Padang." Jurnal Family Education 4, no. 1 (2024): 1–7. http://dx.doi.org/10.24036/jfe.v4i1.150.

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This research is carried out every day the implementation of activities carried out in Kindergarten Pertiwi 3 Of the Padang Governor's Office held knock play activities in the classroom so that every day children feel interesting activities and do not get bored for children for the development of children's musical intelligence. This research aims to get an overview of the development of early childhood musical intelligence in Kindergarten Pertiwi 3 Padang Governor's Office. This type of research is qualitative research using a descriptive approach. To see how the development of early childhoo

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Reddy, D. Samba, and Tina Reddy. "Pharmaceutical and Biotechnological Applications of Transgenic Technology." International Journal of Pharmaceutical Sciences and Nanotechnology 11, no. 4 (2018): 4145–53. http://dx.doi.org/10.37285/ijpsn.2018.11.4.1.

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A transgenic animal is a genetically modified species in which researchers have modified an existing gene or genes by genetic engineering techniques. Genetic modification involves the mutation, insertion, or deletion of genes. Mouse is the most widely used mammalian species for creating transgenic lines. There are two types of transgenic animals: (i) gene deleted (“knock-out”) and (ii) gene overexpressed (“knock-in”). The loss or gain of gene activity often causes changes in a mouse's phenotype, which includes appearance, behavior and other observable characteristics. Knockout mice are key ani

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Rai, Arti K. "PHARMACOGENETIC INTERVENTIONS, ORPHAN DRUGS, AND DISTRIBUTIVE JUSTICE: THE ROLE OF COST-BENEFIT ANALYSIS." Social Philosophy and Policy 19, no. 2 (2002): 246–70. http://dx.doi.org/10.1017/s0265052502192107.

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With the human genome mapped, and with the mapping of more than one hundred animal genomes in progress, the amount of genetic data available is increasing exponentially. This exponential increase in data is having an immediate impact on the process of drug development. By using techniques of information technology to manipulate data regarding the genes, proteins, and biochemical pathways associated with various diseases, scientists are beginning to be able to design drugs in a systematic fashion. In the context of any given disease, scientists look to see whether a gene, a protein for which th

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Kheifets, Anatoli S. "Shake-Off Process in Non-Sequential Single-Photon Double Ionization of Closed-Shell Atomic Targets." Atoms 10, no. 3 (2022): 89. http://dx.doi.org/10.3390/atoms10030089.

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Amusia and Kheifets in 1984 introduced a Green’s function formalism to describe the effect of many-electron correlation on the ionization spectra of atoms. Here, we exploit this formalism to model the shake-off (SO) process, leading to the non-sequential single-photon two-electron ionization (double photoionization—DPI) of closed-shell atomic targets. We separate the SO process from another knock-out (KO) mechanism of DPI and show the SO prevalence away from the DPI threshold. We use this kinematic regime to validate our model by making a comparison with more elaborate techniques, such as conv

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BUFF, ROBERT. "WORST-CASE SCENARIOS FOR AMERICAN OPTIONS." International Journal of Theoretical and Applied Finance 03, no. 01 (2000): 25–58. http://dx.doi.org/10.1142/s0219024900000036.

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One approach to cope with uncertain diffusion parameters when pricing options portfolios is to identify the parameters [Formula: see text] in a subset [Formula: see text] of the parameter space which form the worst-case for a particular portfolio. For the sell-side, this leads to a nonlinear algorithm that maximizes the expected liability under the risk-neutral measure. [Formula: see text] depends on the portfolio under consideration. Moreover, the algorithm must take into account that the exposure to [Formula: see text]-risk changes when non-vanilla components such as barrier or American opti

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Dabrowska, Magdalena, Agata Ciolak, Emilia Kozlowska, Agnieszka Fiszer, and Marta Olejniczak. "Generation of New Isogenic Models of Huntington’s Disease Using CRISPR-Cas9 Technology." International Journal of Molecular Sciences 21, no. 5 (2020): 1854. http://dx.doi.org/10.3390/ijms21051854.

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Huntington’s disease (HD) is a fatal neurodegenerative disorder caused by the expansion of CAG repeats in exon 1 of the huntingtin gene (HTT). Despite its monogenic nature, HD pathogenesis is still not fully understood, and no effective therapy is available to patients. The development of new techniques such as genome engineering has generated new opportunities in the field of disease modeling and enabled the generation of isogenic models with the same genetic background. These models are very valuable for studying the pathogenesis of a disease and for drug screening. Here, we report the gener

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Decraene, James, and Thomas Hinze. "A Multidisciplinary Survey of Computational Techniques for the Modelling, Simulation and Analysis of Biochemical Networks." JUCS - Journal of Universal Computer Science 16, no. (9) (2010): 1152–75. https://doi.org/10.3217/jucs-016-09-1152.

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All processes of life are controlled by networks of interacting biochemical components. The purpose of modelling these networks is manifold. From a theoretical point of view it allows the exploration of network structures and dynamics, to find emergent properties or to explain the organisation and evolution of networks. From a practical point of view, in silico experiments can be performed that would be very expensive or impossible to achieve in the laboratory, such as hypothesis-testing with regards to knock-out experiments or overexpression, or checking the validity of a proposed molecular m

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Lujan, Henry, Eric Romer, Richard Salisbury, Saber Hussain, and Christie Sayes. "Determining the Biological Mechanisms of Action for Environmental Exposures: Applying CRISPR/Cas9 to Toxicological Assessments." Toxicological Sciences 175, no. 1 (2020): 5–18. http://dx.doi.org/10.1093/toxsci/kfaa028.

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Abstract Toxicology is a constantly evolving field, especially in the area of developing alternatives to animal testing. Toxicological research must evolve and utilize adaptive technologies in an effort to improve public, environmental, and occupational health. The most commonly cited mechanisms of toxic action after exposure to a chemical or particle test substance is oxidative stress. However, because oxidative stress involves a plethora of genes and proteins, the exact mechanism(s) are not commonly defined. Exact mechanisms of toxicity can be revealed using an emerging laboratory technique

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Rallabandi, Harikrishna Reddy, Manish Kumar Singh, Loren L. Looger, and Swapan K. Nath. "Defining Mechanistic Links Between the Non-Coding Variant rs17673553 in CLEC16A and Lupus Susceptibility." International Journal of Molecular Sciences 26, no. 1 (2025): 314. https://doi.org/10.3390/ijms26010314.

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Systemic lupus erythematosus (SLE) is a complex autoimmune disorder characterized by widespread inflammation and autoantibody production. Its development and progression involve genetic, epigenetic, and environmental factors. Although genome-wide association studies (GWAS) have repeatedly identified a susceptibility signal at 16p13, its fine-scale source and its functional and mechanistic role in SLE remain unclear. We used bioinformatics to prioritize likely functional variants and validated the top candidate through various experimental techniques, including clustered regularly interspaced s

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Nelson, Nicole C. "A Knockout Experiment: Disciplinary Divides and Experimental Skill in Animal Behaviour Genetics." Medical History 59, no. 3 (2015): 465–85. http://dx.doi.org/10.1017/mdh.2015.30.

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In the early 1990s, a set of new techniques for manipulating mouse DNA allowed researchers to ‘knock out’ specific genes and observe the effects of removing them on a live mouse. In animal behaviour genetics, questions about how to deploy these techniques to study the molecular basis of behaviour became quite controversial, with a number of key methodological issues dissecting the interdisciplinary research field along disciplinary lines. This paper examines debates that took place during the 1990s between a predominately North American group of molecular biologists and animal behaviourists ar

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Yu, Zhixiong, Yumeng Dai, Tingting Li, et al. "A Novel Pathway of Chlorimuron-Ethyl Biodegradation by Chenggangzhangella methanolivorans Strain CHL1 and Its Molecular Mechanisms." International Journal of Molecular Sciences 23, no. 17 (2022): 9890. http://dx.doi.org/10.3390/ijms23179890.

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Chlorimuron-ethyl is a widely used herbicide in agriculture. However, uncontrolled chlorimuron-ethyl application causes serious environmental problems. Chlorimuron-ethyl can be effectively degraded by microbes, but the underlying molecular mechanisms are not fully understood. In this study, we identified the possible pathways and key genes involved in chlorimuron-ethyl degradation by the Chenggangzhangella methanolivorans strain CHL1, a Methylocystaceae strain with the ability to degrade sulfonylurea herbicides. Using a metabolomics method, eight intermediate degradation products were identifi

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Thomson, Michael J., Sudip Biswas, Nikolaos Tsakirpaloglou, and Endang M. Septiningsih. "Functional Allele Validation by Gene Editing to Leverage the Wealth of Genetic Resources for Crop Improvement." International Journal of Molecular Sciences 23, no. 12 (2022): 6565. http://dx.doi.org/10.3390/ijms23126565.

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Advances in molecular technologies over the past few decades, such as high-throughput DNA marker genotyping, have provided more powerful plant breeding approaches, including marker-assisted selection and genomic selection. At the same time, massive investments in plant genetics and genomics, led by whole genome sequencing, have led to greater knowledge of genes and genetic pathways across plant genomes. However, there remains a gap between approaches focused on forward genetics, which start with a phenotype to map a mutant locus or QTL with the goal of cloning the causal gene, and approaches u

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Domínguez-Santos, Rebeca, Katarina Kosalková, Isabel-Clara Sánchez-Orejas, et al. "Characterization of the Gene Encoding S-adenosyl-L-methionine (AdoMet) Synthetase in Penicillium chrysogenum; Role in Secondary Metabolism and Penicillin Production." Microorganisms 10, no. 1 (2021): 78. http://dx.doi.org/10.3390/microorganisms10010078.

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The filamentous fungus Penicillium chrysogenum (recently reidentified as Penicillium rubens) is used in the industrial production of the β-lactam antibiotic penicillin. There are several mechanisms regulating the production of this antibiotic, acting both at the genetic and epigenetic levels, the latter including the modification of chromatin by methyltransferases. S-adenosyl-L-methionine (AdoMet) is the main donor of methyl groups for methyltransferases. In addition, it also acts as a donor of aminopropyl groups during the biosynthesis of polyamines. AdoMet is synthesized from L-methionine an

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Ellrich, Jens. "Electric Low-Frequency Stimulation of the Tongue Induces Long-Term Depression of the Jaw-Opening Reflex in Anesthetized Mice." Journal of Neurophysiology 92, no. 6 (2004): 3332–37. http://dx.doi.org/10.1152/jn.00156.2004.

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Long-term depression (LTD) of somatosensory processing has been demonstrated in slice preparations of the spinal dorsal horn. Although LTD could be reliably induced in vitro, inconsistent results were encountered when the same types of experiments were conducted in adult animals in vivo. We addressed the hypothesis that LTD of orofacial sensorimotor processing can be induced in mice under general anesthesia. The effects of electric low- and high-frequency conditioning stimulation of the tongue on the sensorimotor jaw-opening reflex (JOR) elicited by electric tongue stimulation were investigate

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Baylis, Howard A., and Rafael P. Vázquez-Manrique. "Reverse Genetic Strategies inCaenorhabditis elegans: Towards Controlled Manipulation of the Genome." Scientific World JOURNAL 11 (2011): 1394–410. http://dx.doi.org/10.1100/tsw.2011.126.

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Caenorhabditis eleganshas a complete annotated genome sequence that is augmented by increasing quantities of data from high-throughput postgenomic analyses. This has led to an increasing need to identify the biological functions of specific genes using reverse genetics, i.e., moving from gene to phenotype. Fundamental to this aim is the ability to alter the structure of particular genes by means that are not accessible to classical genetic strategies. Thus, one dream ofC. elegansresearchers is to establish a toolkit for the controlled manipulation of anylociwithin the genome. AlthoughC. elegan

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Kmita, Angelika, Rafał Dańko, Mariusz Holtzer, et al. "Eco-Friendly Inorganic Binders: A Key Alternative for Reducing Harmful Emissions in Molding and Core-Making Technologies." International Journal of Molecular Sciences 25, no. 10 (2024): 5496. http://dx.doi.org/10.3390/ijms25105496.

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Many years of foundry practice and much more accurate analytical methods have shown that sands with organic binders, in addition to their many technological advantages, pose risks associated with the emission of many compounds, including harmful ones (e.g., formaldehyde, phenol, benzene, polycyclic aromatic hydrocarbons, and sulfur), arising during the pouring of liquid casting alloys into molds, their cooling, and knock-out. The aim of this research is to demonstrate the potential benefits of adopting inorganic binders in European iron foundries. This will improve the environmental and workin

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Dalla Costa, Lorenza, Daniela Vinciguerra, Lisa Giacomelli, et al. "Integrated approach for the molecular characterization of edited plants obtained via Agrobacterium tumefaciens-mediated gene transfer." European Food Research and Technology 248, no. 1 (2021): 289–99. http://dx.doi.org/10.1007/s00217-021-03881-0.

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AbstractAgrobacterium tumefaciens-mediated gene transfer—actually the most used method to engineer plants—may lead to integration of multiple copies of T-DNA in the plant genome, as well as to chimeric tissues composed of modified cells and wild type cells. A molecular characterization of the transformed lines is thus a good practice to select the best ones for further investigation. Nowadays, several quantitative and semi-quantitative techniques are available to estimate the copy number (CN) of the T-DNA in genetically modified plants. In this study, we compared three methods based on (1) rea

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Shen, Hui, Ying Zhou, Changguang Liao, et al. "The AlkB Homolog SlALKBH10B Negatively Affects Drought and Salt Tolerance in Solanum lycopersicum." International Journal of Molecular Sciences 25, no. 1 (2023): 173. http://dx.doi.org/10.3390/ijms25010173.

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ALKBH proteins, the homologs of Escherichia coli AlkB dioxygenase, constitute a single-protein repair system that safeguards cellular DNA and RNA against the harmful effects of alkylating agents. ALKBH10B, the first discovered N6-methyladenosine (m6A) demethylase in Arabidopsis (Arabidopsis thaliana), has been shown to regulate plant growth, development, and stress responses. However, until now, the functional role of the plant ALKBH10B has solely been reported in arabidopsis, cotton, and poplar, leaving its functional implications in other plant species shrouded in mystery. In this study, we

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Fertaki, Stefani, Panagiota Giannoutsou, and Malvina G. Orkoula. "Combining Raman Microspectroscopy and X-ray Microcomputed Tomography for the Study of Bone Quality in Apolipoprotein-Deficient Animal Models." Molecules 28, no. 20 (2023): 7196. http://dx.doi.org/10.3390/molecules28207196.

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Raman microspectroscopy and X-ray microcomputed tomography (micro-CT) were used for assessment of the quality of the femur and tibia bones in apolipoprotein-deficient mice compared to control littermates. The cortical and trabecular bone was investigated separately. Raman spectra revealed no differences in the bioapatite-to-collagenous matrix ratio of the cortical bone. The quantities of calcium and collagen, which were measured using atomic absorption spectrometry and thermogravimetric analysis, respectively, were also found to be equal in the two groups. Density and morphometric parameters,

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Martín-Valmaseda, Marina, Sama Rahimi Devin, Germán Ortuño-Hernández, et al. "CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding." International Journal of Molecular Sciences 24, no. 23 (2023): 16656. http://dx.doi.org/10.3390/ijms242316656.

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CRISPR (short for “Clustered Regularly Interspaced Short Palindromic Repeats”) is a technology that research scientists use to selectively modify the DNA of living organisms. CRISPR was adapted for use in the laboratory from the naturally occurring genome-editing systems found in bacteria. In this work, we reviewed the methods used to introduce CRISPR/Cas-mediated genome editing into fruit species, as well as the impacts of the application of this technology to activate and knock out target genes in different fruit tree species, including on tree development, yield, fruit quality, and toleranc

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Crespel, Laurent, Camille Le Bras, Bénédicte Dubuc, et al. "Divergent Mechanisms of Internode Elongation in Response to Far-Red in Two Rose Genotypes." Plants 14, no. 7 (2025): 1115. https://doi.org/10.3390/plants14071115.

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The quality of potted ornamental plants depends on their architecture, which should be compact and branched. Among the techniques for controlling this architecture, LED lighting, by manipulating light quality, offers an effective means of regulating elongation and branching. In rose, the addition of far-red (FR) light stimulated branching but induced excessive stem elongation, i.e., internode elongation. However, some varieties remained insensitive to this effect, demonstrating phenotypic stability. This study investigated the underlying mechanisms of internode elongation in response to FR in

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