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Artykuły w czasopismach na temat „Transfection”

Autor: Grafiati

Data publikacji: 4 czerwca 2021

Data aktualizacji: 25 lipca 2025

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1

Cheow, Pheik-Sheen, Tiong Kit Tan, Adelene Ai-Lian Song, Khatijah Yusoff, and Suet Lin Chia. "An improved method for the rescue of recombinant Newcastle disease virus." BioTechniques 68, no. 2 (2020): 96–100. http://dx.doi.org/10.2144/btn-2019-0110.

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Reverse genetics has been used to generate recombinant Newcastle disease virus with enhanced immunogenic properties for vaccine development. The system, which involves co-transfecting the viral antigenomic plasmid with three helper plasmids into a T7 RNA polymerase-expressing cell to produce viral progenies, poses a great challenge. We have modified the standard transfection method to improve the transfection efficiency of the plasmids, resulting in a higher titer of virus progeny production. Two transfection reagents (i.e., lipofectamine and polyethylenimine) were used to compare the transfec

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2

Zhang, Jianxiong, Yawei Hu, Xiaoqing Wang, Peng Liu, and Xiaofang Chen. "High-Throughput Platform for Efficient Chemical Transfection, Virus Packaging, and Transduction." Micromachines 10, no. 6 (2019): 387. http://dx.doi.org/10.3390/mi10060387.

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Intracellular gene delivery is normally required to study gene functions. A versatile platform able to perform both chemical transfection and viral transduction to achieve efficient gene modification in most cell types is needed. Here we demonstrated that high throughput chemical transfection, virus packaging, and transduction can be conducted efficiently on our previously developed superhydrophobic microwell array chip (SMAR-chip). A total of 169 chemical transfections were successfully performed on the chip in physically separated microwells through a few simple steps, contributing to the co

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3

Toth, Maria, Manuel Reithofer, Gregory Dutra, Patricia Pereira Aguilar, Astrid Dürauer, and Reingard Grabherr. "Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells." Viruses 16, no. 3 (2024): 426. http://dx.doi.org/10.3390/v16030426.

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(1) Recombinant protein production in mammalian cells is either based on transient transfection processes, often inefficient and underlying high batch-to-batch variability, or on laborious generation of stable cell lines. Alternatively, BacMam, a transduction process using the baculovirus, can be employed. (2) Six transfecting agents were compared to baculovirus transduction in terms of transient and stable protein expression characteristics of the model protein ACE2-eGFP using HEK293-6E, CHO-K1, and Vero cell lines. Furthermore, process optimization such as expression enhancement using sodium

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Gam, Jeremy J., Breanna DiAndreth, Ross D. Jones, Jin Huh, and Ron Weiss. "A ‘poly-transfection’ method for rapid, one-pot characterization and optimization of genetic systems." Nucleic Acids Research 47, no. 18 (2019): e106-e106. http://dx.doi.org/10.1093/nar/gkz623.

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Abstract Biological research is relying on increasingly complex genetic systems and circuits to perform sophisticated operations in living cells. Performing these operations often requires simultaneous delivery of many genes, and optimizing the stoichiometry of these genes can yield drastic improvements in performance. However, sufficiently sampling the large design space of gene expression stoichiometries in mammalian cells using current methods is cumbersome, complex, or expensive. We present a ‘poly-transfection’ method as a simple yet high-throughput alternative that enables comprehensive

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5

Gardiner, Donald L., Tina S. Skinner-Adams, Tobias Spielmann, and Katharine R. Trenholme. "Malaria transfection and transfection vectors." Trends in Parasitology 19, no. 9 (2003): 381–83. http://dx.doi.org/10.1016/s1471-4922(03)00187-9.

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6

Peng, Zhiqiang, Jianping Xiong, Hanzhi Dong, and Wuping Li. "Preparation of Polymer Nanocarrier Material and Its Application in B-Cell Lymphoma." Science of Advanced Materials 12, no. 10 (2020): 1524–34. http://dx.doi.org/10.1166/sam.2020.3877.

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The β-cyclodextrin (β-CD) is coupled with polyethyleneimine (PEI 600 Da) to produce a polymer nanocarrier material (PyD-W) with good biocompatibility and high transfection rate. First, the test was performed to study the influence of different factors on the transfection efficiency of PyD-W materials in terms of cell type and transfection system. Then the effect of adding wheat germ agglutinin on the material-cell membrane binding when transfecting cells by PyD-W materials was studied. The influence of temperature and cell phagocytosis inhibitors on the entire transfection process were taken i

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7

Yin, Jingwen, and Henry R. Henney Jr. "Stable transfection ofAcanthamoeba." Canadian Journal of Microbiology 43, no. 3 (1997): 239–44. http://dx.doi.org/10.1139/m97-033.

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The promoter activity of an Acanthamoeba polyubiquitin gene was analyzed in its homologous system. A modified calcium phosphate transfection method using a neomycin marker vector was developed to achieve highly efficient transfection of the Acanthamoeba polyubiquitin gene into Acanthamoeba cells. In this transfection procedure, the calcium phosphate – DNA complex was formed gradually in the medium during incubation with cells and precipitated on the cells. The crucial factors for obtaining efficient transfection were the pH (6.95) of the transfection buffer used for the calcium phosphate preci

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8

Nie, Leng, Li Zeng Gao, Xi Yun Yan, and Tai Hong Wang. "Functionalized Tetrapod-Like ZnO Nanostructrures for DNA Gene Delivery." Solid State Phenomena 121-123 (March 2007): 747–50. http://dx.doi.org/10.4028/www.scientific.net/ssp.121-123.747.

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Amino-modified tetrapod-like ZnO nanostructures were tried as novel carriers for mammalian cell transfections. The nanostructures consisted of four needle-shaped tetrahedrally arranged legs connected at the center. After silica coating and amino modification, ZnO nanostructures complexes bound plasmid DNA through electrostatic interactions in aqueous solution. When mixed with cells, DNA-nanostructures attached easily onto cell membranes and entered the cells for gene expressions. Due to high positive charge densities on surfaces and needle-shaped tetrahedral structures, functionalized ZnO used

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9

Chung, Namjin, Louis Locco, Kevin W. Huff, et al. "An Efficient and Fully Automated High-Throughput Transfection Method for Genome-Scale siRNA Screens." Journal of Biomolecular Screening 13, no. 2 (2008): 142–48. http://dx.doi.org/10.1177/1087057107312032.

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RNA interference (RNAi), combined with the availability of genome sequences, provides an unprecedented opportunity for the massive and parallel investigations of gene function. Small interfering RNA (siRNA) represents a popular and quick approach of RNAi for in vitro loss-of-function genetic screens. Efficient transfection of siRNA is critical for unambiguous interpretation of screen results and thus overall success of any siRNA screen. A high-throughput, lipid-based transfection method for siRNA was developed that can process eighty 384-well microplates in triplicate (for a total of 30,720 un

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10

Chong, Kevin Wai Yin, Alan Yiu-Wah Lee, Evelyn S. C. Koay, Sze Jee Seet, and Nam Sang Cheung. "pH dependent high transfection efficiency of mouse neuroblastomas using TransFectin." Journal of Neuroscience Methods 158, no. 1 (2006): 56–63. http://dx.doi.org/10.1016/j.jneumeth.2006.05.017.

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11

Moradian, Hanieh, Andreas Lendlein, and Manfred Gossen. "Strategies for simultaneous and successive delivery of RNA." Journal of Molecular Medicine 98, no. 12 (2020): 1767–79. http://dx.doi.org/10.1007/s00109-020-01956-1.

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AbstractAdvanced non-viral gene delivery experiments often require co-delivery of multiple nucleic acids. Therefore, the availability of reliable and robust co-transfection methods and defined selection criteria for their use in, e.g., expression of multimeric proteins or mixed RNA/DNA delivery is of utmost importance. Here, we investigated different co- and successive transfection approaches, with particular focus on in vitro transcribed messenger RNA (IVT-mRNA). Expression levels and patterns of two fluorescent protein reporters were determined, using different IVT-mRNA doses, carriers, and

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12

Cheung, Winston Y., Owen Hovey, Jonathan M. Gobin, et al. "Efficient Nonviral Transfection of Human Bone Marrow Mesenchymal Stromal Cells Shown Using Placental Growth Factor Overexpression." Stem Cells International 2018 (December 24, 2018): 1–10. http://dx.doi.org/10.1155/2018/1310904.

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Background. Human mesenchymal stromal/stem cells (hMSCs) hold great therapeutic potential due to their immunomodulatory and tissue regenerative properties. Enhancement of biological features of hMSCs by transfection has become a focus of investigation for cell- and gene-based therapies. However, many of the current transient transfection methods result in either low transfection efficiency or high cytotoxicity. Methods. In order to find a transfection method that would address the current issues of low transfection efficiency and high cytotoxicity, 6 commercially available cationic lipid and p

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13

Maddila, Santhosh Chandar, Chandrashekhar Voshavar, Porkizhi Arjunan, et al. "Cholesterol Sequestration from Caveolae/Lipid Rafts Enhances Cationic Liposome-Mediated Nucleic Acid Delivery into Endothelial Cells." Molecules 26, no. 15 (2021): 4626. http://dx.doi.org/10.3390/molecules26154626.

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Delivering nucleic acids into the endothelium has great potential in treating vascular diseases. However, endothelial cells, which line the vasculature, are considered as sensitive in nature and hard to transfect. Low transfection efficacies in endothelial cells limit their potential therapeutic applications. Towards improving the transfection efficiency, we made an effort to understand the internalization of lipoplexes into the cells, which is the first and most critical step in nucleic acid transfections. In this study, we demonstrated that the transient modulation of caveolae/lipid rafts me

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14

Li, Chen, Biao Qian, Zhao Ni, et al. "Construction of recombinant lentiviral vector containing human stem cell leukemia gene and its expression in interstitial cells of cajal." Open Life Sciences 15, no. 1 (2020): 83–91. http://dx.doi.org/10.1515/biol-2020-0010.

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AbstractThis study aims to construct recombinant lentiviral vectors containing the human stem cell leukemia (SCL) gene and investigate their in vitro transfection efficiency in Interstitial Cells of Cajal (ICC) of guinea pig bladders. In this study, the human SCL gene was successfully cloned, and the recombinant lentivirus GV287-SCL was successfully constructed. The titer of the recombinant lentivirus was 5 × 108 TU /mL. After transfecting the ICCs with the lentiviral vector at different MOIs, the optimal MOI was determined to be 10.0, and the optimal transfection time was determined to be 3 d

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15

Malhotra, Shreya, Priyanka Singh, Aseem Tara, et al. "Effect of custom-designed transfection buffer on delivery of genome modification components into primary cells of buffalo, cattle, goats, and sheep." Revista Científica de la Facultad de Ciencias Veterinarias 33, Suplemento (2023): 279–80. https://doi.org/10.52973/rcfcv-wbc123.

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The transfer of genome-modification components into farm animal cells is indispensable for the production of genome-modified and transgenic farm animals. Electroporation is a physical transfection method when appropriately used; this technique is safe, simple to use, affordable, and efficient in transfecting cells from several lineages. Electroporation efficiency depends on various physical parameters, of which cell type is considered a major factor for transfection efficiency. Primary cells are generally less susceptible to transfection than other cell types due to their finite lifespan and l

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16

Dokka, Sujatha, David Toledo, Liying Wang, et al. "Free radical-mediated transgene inactivation of macrophages by endotoxin." American Journal of Physiology-Lung Cellular and Molecular Physiology 279, no. 5 (2000): L878—L883. http://dx.doi.org/10.1152/ajplung.2000.279.5.l878.

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Endotoxin, the lipopolysaccharide component of gram-negative bacteria, is a common contaminant of plasmid DNA preparations. The present study investigated the effect of endotoxin on gene transfection efficiency and the role of reactive oxygen species (ROS) in this process. Gene transfection studies were performed in various cell types with cytomegalovirus-luciferase as a reporter plasmid and cationic liposome as a transfecting agent. The presence of endotoxin in plasmid DNA preparations severely limited transgene expression in macrophages but had little or no effect in other cell types tested.

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17

Shen, Wen-Juan, Duo-Mei Tian, Le Fu, et al. "Elastin-Derived VGVAPG Fragment Decorated Cell-Penetrating Peptide with Improved Gene Delivery Efficacy." Pharmaceutics 15, no. 2 (2023): 670. http://dx.doi.org/10.3390/pharmaceutics15020670.

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Cell-penetrating peptides (CPPs) are attractive non-viral gene delivery vectors due to their high transfection capacity and safety. Previously, we have shown that cell-penetrating peptide RALA can be a promising gene delivery vector for chronic wound regeneration application. In this study, we engineered a novel peptide called RALA-E by introducing elastin-derived VGVAPG fragment into RALA, in order to target the elastin-binding protein on the cell surface and thus improve delivery efficacy of RALA. The transfection efficiency of RALA-E was evaluated by transfecting the HEK-293T and HeLa cell

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18

Nybo, Kristie. "Retroviral Transfection." BioTechniques 53, no. 2 (2012): 79–80. http://dx.doi.org/10.2144/000113901.

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19

Kumar, Priti, Arvindhan Nagarajan, and Pradeep D. Uchil. "Optical Transfection." Cold Spring Harbor Protocols 2018, no. 12 (2018): pdb.top096222. http://dx.doi.org/10.1101/pdb.top096222.

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20

Heng, Zealyn Shi-Lin, Joshua Yi Yeo, Darius Wen-Shuo Koh, Samuel Ken-En Gan, and Wei-Li Ling. "Augmenting recombinant antibody production in HEK293E cells: optimizing transfection and culture parameters." Antibody Therapeutics 5, no. 1 (2022): 30–41. http://dx.doi.org/10.1093/abt/tbac003.

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Abstract Background Optimizing recombinant antibody production is important for cost-effective therapeutics and diagnostics. With impact on commercialization, higher productivity beyond laboratory scales is highly sought, where efficient production can also accelerate antibody characterizations and investigations. Methods Investigating HEK293E cells for mammalian antibody production, various transfection and culture parameters were systematically analyzed for antibody light chain production before evaluating them for whole antibody production. Transfection parameters investigated include seedi

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21

Sanderson, Theo, and Julian C. Rayner. "PhenoPlasm: a database of disruption phenotypes for malaria parasite genes." Wellcome Open Research 2 (June 21, 2017): 45. http://dx.doi.org/10.12688/wellcomeopenres.11896.1.

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Two decades after the first Plasmodium transfection, attempts have been made to disrupt more than 3,151 genes in malaria parasites, across five Plasmodium species. While results from rodent malaria transfections have been curated and systematised, empowering large-scale analysis, phenotypic data from human malaria parasite transfections currently exists as individual reports scattered across a the literature. To facilitate systematic analysis of published experimental genetic data across Plasmodium species, we have built PhenoPlasm (http://www.phenoplasm.org), a database of phenotypes generate

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22

Sanderson, Theo, and Julian C. Rayner. "PhenoPlasm: a database of disruption phenotypes for malaria parasite genes." Wellcome Open Research 2 (July 24, 2017): 45. http://dx.doi.org/10.12688/wellcomeopenres.11896.2.

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Two decades after the first Plasmodium transfection, attempts have been made to disrupt more than 3,151 genes in malaria parasites, across five Plasmodium species. While results from rodent malaria transfections have been curated and systematised, empowering large-scale analysis, phenotypic data from human malaria parasite transfections currently exists as individual reports scattered across a the literature. To facilitate systematic analysis of published experimental genetic data across Plasmodium species, we have built PhenoPlasm (http://www.phenoplasm.org), a database of phenotypes generate

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23

Bodbin, Sara E., Chris Denning, and Diogo Mosqueira. "Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent." Methods and Protocols 3, no. 3 (2020): 57. http://dx.doi.org/10.3390/mps3030057.

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Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection efficiency is a barrier to wider realisation of the potential this model system has to offer. We endeavoured to produce a protocol for improved transfection of hPSC-CMs using the ViafectTM reagent by Promega. Through optimisation of four essential parameters: (i) serum supplementation, (ii) time between replating and transfection, (iii) reagent to DNA r

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24

Zhang, Guohong, and Steven R. Kain. "Transfection Maximizer Increases the Efficiency of Calcium Phosphate Transfections with Mammalian Cells." BioTechniques 21, no. 5 (1996): 940–45. http://dx.doi.org/10.2144/96215pf02.

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Tinsley, John H., James Hawker, and Yuan Yuan. "Efficient protein transfection of cultured coronary venular endothelial cells." American Journal of Physiology-Heart and Circulatory Physiology 275, no. 5 (1998): H1873—H1878. http://dx.doi.org/10.1152/ajpheart.1998.275.5.h1873.

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Although it is well recognized that microvascular endothelial cells play an important role in the local regulation of tissue perfusion and exchange processes, the precise effect of specific endothelial proteins on microvascular function remains to be elucidated. The lack of information is partially due to methodological limitations, because pharmacological approaches that are routinely used in conventional microcirculatory studies produce nonspecific information. The purpose of this study was to develop an efficient method of transfecting endothelial cells with proteins for functional analysis

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Kościańska, E., and K. Wypijewski. "Electroporated intact BY-2 tobacco culture cells as a model of transient expression study." Acta Biochimica Polonica 48, no. 3 (2001): 657–61. http://dx.doi.org/10.18388/abp.2001_3900.

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Transfer of foreign genes into plant cells can be accomplished by several methods: agrobacterium-mediated, microinjection, biolistic particle bombardment and electroporation. The last one is frequently used for transfection of plant protoplasts for transient gene expression. Electroporation is a simple procedure and allows transfecting a large number of cells at one time. Square wave-modulated porators are the most efficient for introducing expression cassettes into plant protoplasts. Based on a protocol developed by Wu & Feng (Plant Cell Reports, 1999, 18, 381-386), we optimized condition

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27

Liu, Dexi, Tan Ren, and Xiang Gao. "Cationic Transfection Lipids." Current Medicinal Chemistry 10, no. 14 (2003): 1307–15. http://dx.doi.org/10.2174/0929867033457386.

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Dean, David A., and Joshua Z. Gasiorowski. "Liposome-Mediated Transfection." Cold Spring Harbor Protocols 2011, no. 3 (2011): prot5583. http://dx.doi.org/10.1101/pdb.prot5583.

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Dean, David A., and Joshua Z. Gasiorowski. "Dendrimer-Mediated Transfection." Cold Spring Harbor Protocols 2011, no. 3 (2011): prot5584. http://dx.doi.org/10.1101/pdb.prot5584.

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Toma, Tudor. "Transfection using lasers." Genome Biology 3 (2002): spotlight—20020725–01. http://dx.doi.org/10.1186/gb-spotlight-20020725-01.

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Wesson, Dawn M., and Donald J. Krogstad. "Transfection and malaria." Nature Medicine 1, no. 8 (1995): 745–47. http://dx.doi.org/10.1038/nm0895-745.

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Won, Rachel. "Single-cell transfection." Nature Photonics 7, no. 10 (2013): 762. http://dx.doi.org/10.1038/nphoton.2013.260.

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Kumar, Priti, Arvindhan Nagarajan, and Pradeep D. Uchil. "DEAE-Dextran Transfection." Cold Spring Harbor Protocols 2018, no. 7 (2018): pdb.top096263. http://dx.doi.org/10.1101/pdb.top096263.

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Moguel, Bárbara, Raúl J. Bobes, Julio C. Carrero, and Juan P. Laclette. "Transfection of Platyhelminthes." BioMed Research International 2015 (2015): 1–9. http://dx.doi.org/10.1155/2015/206161.

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Flatworms are one of the most diverse groups within Lophotrochozoa with more than 20,000 known species, distributed worldwide in different ecosystems, from the free-living organisms in the seas and lakes to highly specialized parasites living in a variety of hosts, including humans. Several infections caused by flatworms are considered major neglected diseases affecting countries in the Americas, Asia, and Africa. For several decades, a particular interest on free-living flatworms was due to their ability to regenerate considerable portions of the body, implying the presence of germ cells that

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35

Roberts, Josh P. "Tackling Transfection Tactically." Genetic Engineering & Biotechnology News 32, no. 5 (2012): 36–37. http://dx.doi.org/10.1089/gen.32.5.15.

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McKenna, Neil. "Tackling Transfection Tasks." Genetic Engineering & Biotechnology News 33, no. 1 (2013): 1, 22, 24. http://dx.doi.org/10.1089/gen.33.01.13.

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Labant, Mary Ann. "Transfection Methods Evolving." Genetic Engineering & Biotechnology News 33, no. 15 (2013): 1, 32, 33, 34,. http://dx.doi.org/10.1089/gen.33.15.11.

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Roulland-Dussoix, Daisy. "Transfection ofCorynebacterium diphtheriae." Current Microbiology 18, no. 5 (1989): 319–21. http://dx.doi.org/10.1007/bf01575948.

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Rose, John K. "Optimization of Transfection." Current Protocols in Neuroscience 1, no. 1 (1997): A.1B.1—A.1B.3. http://dx.doi.org/10.1002/0471142301.nsa01bs01.

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Kingston, Robert E., Claudia A. Chen, and Hiroto Okayama. "Calcium Phosphate Transfection." Current Protocols in Neuroscience 1, no. 1 (1997): A.1C.1—A.1C.8. http://dx.doi.org/10.1002/0471142301.nsa01cs01.

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Potter, Huntington. "Transfection by Electroporation." Current Protocols in Neuroscience 1, no. 1 (1997): A.1E.1—A.1E.5. http://dx.doi.org/10.1002/0471142301.nsa01es01.

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Pitt, William G., Daniel R. Jack, Ghaleb A. Husseini, and Brett V. Memmott. "Non-Viral Gene Transfection with Ultrasound: Is 100% Transfection Possible?" Advanced Science Letters 11, no. 1 (2012): 98–105. http://dx.doi.org/10.1166/asl.2012.2161.

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Nimesh, Surendra, Amit Saxena, Ajeet Kumar, and Ramesh Chandra. "Improved transfection efficiency of chitosan-DNA complexes employing reverse transfection." Journal of Applied Polymer Science 124, no. 3 (2011): 1771–77. http://dx.doi.org/10.1002/app.35179.

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Yano, Motoki, Yoshiaki Nakashima, Yoshihiro Kobayashi, et al. "Endostatin gene transfection using a cationic lipid: advantages of transfection before tumor cell inoculation and repeated transfection." Cancer Gene Therapy 11, no. 5 (2004): 354–62. http://dx.doi.org/10.1038/sj.cgt.7700704.

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da Silva, Zigomar, Andressa Pereira de Souza, José Rodrigo Claudio Pandolfi, Francisco Noé da Fonseca, Carlos André da Veiga Lima-Rosa, and Mariana Groke Marques. "Comparison between electroporation and polyfection in pig sperm: efficiency and cell viability implications." Zygote 26, no. 4 (2018): 286–93. http://dx.doi.org/10.1017/s0967199418000205.

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SummaryThe aim of this study was to optimize protocols for electroporation (EP) and polyfection (PLF) using polyethyleneimine (PEI) for pig sperm transfection and to determine which method was the most efficient. For EP standardization, different voltages, amounts and times of electric pulses were tested using propidium iodide (PI) as reporter. For PLF standardization, different concentrations of fluorescein isothiocyanate (FITC)-labelled PEI (PEI/FITC) were incubated with sperm for different periods of time. Flow cytometry was performed to evaluate the best protocol in terms of cell viability

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Sturm, Lisa, Bettina Schwemberger, Ursula Menzel, et al. "In Vitro Evaluation of a Nanoparticle-Based mRNA Delivery System for Cells in the Joint." Biomedicines 9, no. 7 (2021): 794. http://dx.doi.org/10.3390/biomedicines9070794.

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Biodegradable and bioresponsive polymer-based nanoparticles (NPs) can be used for oligonucleotide delivery, making them a promising candidate for mRNA-based therapeutics. In this study, we evaluated and optimized the efficiency of a cationic, hyperbranched poly(amidoamine)s-based nanoparticle system to deliver tdTomato mRNA to primary human bone marrow stromal cells (hBMSC), human synovial derived stem cells (hSDSC), bovine chondrocytes (bCH), and rat tendon derived stem/progenitor cells (rTDSPC). Transfection efficiencies varied among the cell types tested (bCH 28.4% ± 22.87, rTDSPC 18.13% ±

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Tsan, M. F., J. E. White, and B. Shepard. "Lung-specific direct in vivo gene transfer with recombinant plasmid DNA." American Journal of Physiology-Lung Cellular and Molecular Physiology 268, no. 6 (1995): L1052—L1056. http://dx.doi.org/10.1152/ajplung.1995.268.6.l1052.

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A number of gene delivery methods have been developed to facilitate gene transfer into mammalian somatic cells in vivo. In this study, we demonstrated that tracheal insufflation of two recombinant plasmids containing a bacterial gene, chloramphenicol acetyltransferase (CAT), driven by the cytomegalovirus (CMV) immediate early promoter/enhancer, either alone or complexed to cationic liposomes, resulted in efficient and selective transfection of the lungs in rats. When the simian virus 40 (SV40) promoter/enhancer was used, there was no detectable transfection. Insufflation of plasmid DNA was as

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Van der Loo, Johannes C. M., William Swaney, Diana Nordling, et al. "Production of High Titer cGMP-Grade SIN Gamma-Retroviral Vectors by Transfection in a Closed System Bioreactor." Blood 112, no. 11 (2008): 3539. http://dx.doi.org/10.1182/blood.v112.11.3539.3539.

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Abstract The need for gamma-retroviral vectors with self-inactivating (SIN) long terminal repeats for clinical trials has prompted a shift in the method with which large scale GMP-grade vectors are produced, from the use of stable producer lines to transient transfection-based techniques. The main challenge of instituting this methodology was to develop SIN retrovirus vectors that produced high amounts of genomic vector RNA in packaging cells, and to design scalable processes for closed system culture, transfection and virus harvest. Using improved expression plasmids, the Vector Production Fa

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Putri, Rizka Rahmana, and Christian Larbi Ayisi. "Transfection Of Difficult-To-Transfect Zebrafish (Danio rerio) ZF4 Cells Using Chemical Transfection and Nucleofection Method." Juvenil:Jurnal Ilmiah Kelautan dan Perikanan 4, no. 2 (2023): 104–8. http://dx.doi.org/10.21107/juvenil.v4i2.19586.

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ABSTRACTTransfection is a powerful tool for introducing foreign DNA into target cells. Many kits and methods are offered to simplify the process of introducing foreign DNA into cells. ZF4 is a cell type derived from zebrafish that is difficult to transfect. The majority of studies involving transfection into cells require transfection efficiency 70%. Our research demonstrates transfection using 2 kinds of methods, namely chemical transfection using X-tremeGene HP and electroporation-based transfection using a nucleofector device which will later be called Nucleofection. Our results show that t

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Villa-Martínez, Elisa, Amelia Rios, Roxana Gutiérrez-Vidal, and Bruno Escalante. "Potentiation of anti-angiogenic eNOS-siRNA transfection by ultrasound-mediated microbubble destruction in ex vivo rat aortic rings." PLOS ONE 19, no. 8 (2024): e0308075. http://dx.doi.org/10.1371/journal.pone.0308075.

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Nitric oxide (NO) regulates vascular homeostasis and plays a key role in revascularization and angiogenesis. The endothelial nitric oxide synthase (eNOS) enzyme catalyzes NO production in endothelial cells. Overexpression of the eNOS gene has been implicated in pathologies with dysfunctional angiogenic processes, such as cancer. Therefore, modulating eNOS gene expression using small interfering RNAs (siRNAs) represents a viable strategy for antitumor therapy. siRNAs are highly specific to the target gene, thus reducing off-target effects. Given the widespread distribution of endothelium and th

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